Rh2CG171800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0015376
Physical Location & Seq
Forward (+)
15363077 .. 15365490
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG171800.1

Sequence Viewer

Length: 1146 bp
ATGATGATCTTAACCATGGCCATAATAAGATCTTATATTACTCTGGCCTTTGTAGGCTTGATTCTTTTGCCCATCAACAACCTGAACTTTTTGGCATATGCTGCTGAGAGTGAAGGGCTGGGAGCTTCTTTTATCTTTGGTGATTCCCTGGTTGATTGCGGAAACAATAACTACTTGTCGACATTGTCCAAGGCAAATATTCGTCCTAATGGGATGGATTTCCAGCCATCTGGAGGAAATCCCACCGGGCGGTTCACCAATGGTAGAACCATTGGTGATATAATTGGTGAAGAATTGGGACAACCAAACTATGCAGTTCCATTTTTAGCTCCTAACGCTACAGGGAAAGCAATATTGTATGGGGTGAATTATGCTTCAGGAGGAGCAGGGATCATGAATGCAACAGGAAGAATATTTGTAAATAGGGTGGGGATGGATATCCAAATCGATTATTTCAACGAGACAAGGAAGCAGATTGATAAGCTATTGGGTCCATCAAAGGCAAAAGAGTACATTTCGAAAAGGTCTATTTTCTCGATCACAGTTGGAGCAAATGACTTTCTGAACAATTACCTACTACCAGTCCTATCCATTGGAGCAAGAATTTCTGAGACCCCAGATGCATTCATTGATGATTTGATCACTCACTTCAGGGCTCAACTTACTAGACTTTATCAATTGGGTGCTCGGAAATTTGTGATAGGAAATGTTGGACCAATAGGTTGCATACCTTACCAGAAGACTATCAATCAGCTTGACCAAAACCAATGTGTGGAGTTGCCTAATAAGCTTGCGCTGCAGTACAATGGCAGATTGAAGGACTTGGTTGCTGAGCTGAATGATAACCTCCCTGGATCAACATTTGTTTATGCAAATGTGTATGATCTAGTGTCGGACCTCATTACCAACTACGAAAAATATGGATTTTCAACAGCAAGTAGAGCTTGTTGTGGAAATGGTGGCCAATATGCAGGAATAGTTCCATGTGGTCCAACATCAAGTCTGTGTACCGATCGGTCCAAGCATGTATTCTGGGATCCCTACCATCCAAGTGAAGCTGCCAACATTATACTCGCCAAAAAATTGCTTGATGGAGACACAAGAGTTATTTCTCCAATGAATCTCAGGCAACTTCGAGACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

381

Amino Acids

41.64

Weight (kDa)

7.54

Isoelectric Point (pI)

29.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 44 - 362 4.9e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23540
fragaria_vesca FvH4_1g14780
malus_domestica MD02G1161000.v1.1 MD15G1275100.v1.1
prunus_persica Prupe.7G142600_v2.0.a1
pyrus_communis pycom02g12710 pycom15g23820
rosa_chinensis RchiOBHm_Chr2g0103741
rosa_laevigata RLG00000017253
rosa_multiflora Rmu_sc0001852.1_g000002
rosa_roxburghii Rroxscaffold_2G00139440
rosa_rugosa Rorug02G0117000
rosa_samantha Rh2AG165800 Rh2BG173100 Rh2CG171800
rosa_wichuraiana Rw2G012990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 772
AccI GTMKAC 1 cut(s) 179
AciI CCGC 2 cut(s) 159, 250
AclWI GGATC 4 cut(s) 398, 862, 1031, 1044
AcoI YGGCCR 2 cut(s) 18, 961
AcsI RAATTY 2 cut(s) 603, 692
AcuI CTGAAG 2 cut(s) 360, 634
AfaI GTAC 3 cut(s) 512, 803, 1009
AfiI CCNNNNNNNGG 3 cut(s) 233, 249, 772
AgsI TTSAA 3 cut(s) 457, 817, 930
AjnI CCWGG 2 cut(s) 147, 850
AjuI GAANNNNNNNTTGG 2 cut(s) 1013, 1045
AluBI AGCT 8 cut(s) 125, 329, 484, 754, 790, 835, 944, 1058
AluI AGCT 8 cut(s) 125, 329, 484, 754, 790, 835, 944, 1058
Alw21I GWGCWC 1 cut(s) 688
Alw26I GTCTC 4 cut(s) 455, 605, 1089, 1131
AlwI GGATC 4 cut(s) 398, 862, 1031, 1044
AoxI GGCC 3 cut(s) 18, 45, 961
ApeKI GCWGC 3 cut(s) 101, 796, 1058
ApoI RAATTY 2 cut(s) 603, 692
AspLEI GCGC 1 cut(s) 796
AspS9I GGNCC 5 cut(s) 491, 713, 895, 989, 1017
AsuC2I CCSGG 1 cut(s) 247
AsuHPI GGTGA 5 cut(s) 152, 247, 287, 299, 376
AsuII TTCGAA 1 cut(s) 518
AvaII GGWCC 5 cut(s) 491, 713, 895, 989, 1017
BalI TGGCCA 2 cut(s) 20, 963
BamHI GGATCC 1 cut(s) 1036
BanII GRGCYC 1 cut(s) 658
BbsI GAAGAC 1 cut(s) 746
Bbv12I GWGCWC 1 cut(s) 688
BbvI GCAGC 3 cut(s) 88, 783, 1045
BccI CCATC 7 cut(s) 80, 208, 235, 427, 502, 1053, 1085
BciT130I CCWGG 2 cut(s) 149, 852
BclI TGATCA 1 cut(s) 639
BcnI CCSGG 1 cut(s) 247
BcoDI GTCTC 4 cut(s) 455, 605, 1089, 1131
BfaI CTAG 2 cut(s) 666, 887
BfmI CTRYAG 2 cut(s) 339, 797
BglII AGATCT 1 cut(s) 29
BisI GCNGC 3 cut(s) 102, 797, 1059
BlpI GCTNAGC 1 cut(s) 831
BlsI GCNGC 3 cut(s) 103, 798, 1060
Bme1390I CCNGG 3 cut(s) 149, 247, 852
Bme18I GGWCC 5 cut(s) 491, 713, 895, 989, 1017
BmgT120I GGNCC 5 cut(s) 491, 713, 895, 989, 1017
BmiI GGNNCC 2 cut(s) 492, 1038
BmrFI CCNGG 3 cut(s) 149, 247, 852
BmsI GCATC 1 cut(s) 610
BpiI GAAGAC 1 cut(s) 746
BpmI CTGGAG 1 cut(s) 252
Bpu1102I GCTNAGC 1 cut(s) 831
Bpu14I TTCGAA 1 cut(s) 518
BpuMI CCSGG 1 cut(s) 247
Bsa29I ATCGAT 1 cut(s) 447
BsaBI GATNNNNATC 1 cut(s) 437
BsaI GGTCTC 2 cut(s) 605, 1131
BsaJI CCNNGG 4 cut(s) 15, 147, 189, 850
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 249, 772
Bse1I ACTGG 1 cut(s) 581
Bse8I GATNNNNATC 1 cut(s) 437
BseBI CCWGG 2 cut(s) 149, 852
BseCI ATCGAT 1 cut(s) 447
BseDI CCNNGG 4 cut(s) 15, 147, 189, 850
BseGI GGATG 3 cut(s) 219, 438, 1045
BseJI GATNNNNATC 1 cut(s) 437
BseLI CCNNNNNNNGG 3 cut(s) 233, 249, 772
BseMII CTCAG 4 cut(s) 96, 600, 822, 1138
BseNI ACTGG 1 cut(s) 581
BseRI GAGGAG 1 cut(s) 396
BseXI GCAGC 3 cut(s) 88, 783, 1045
BseYI CCCAGC 1 cut(s) 118
Bsh1285I CGRYCG 1 cut(s) 1015
BshFI GGCC 3 cut(s) 20, 47, 963
BshVI ATCGAT 1 cut(s) 447
BsiEI CGRYCG 1 cut(s) 1015
BsiHKAI GWGCWC 1 cut(s) 688
BsiSI CCGG 1 cut(s) 246
BslFI GGGAC 1 cut(s) 312
BslI CCNNNNNNNGG 3 cut(s) 233, 249, 772
BsmAI GTCTC 4 cut(s) 455, 605, 1089, 1131
BsmFI GGGAC 1 cut(s) 312
BsmI GAATGC 2 cut(s) 403, 623
BsnI GGCC 3 cut(s) 20, 47, 963
Bso31I GGTCTC 2 cut(s) 605, 1131
Bsp119I TTCGAA 1 cut(s) 518
Bsp1286I GDGCHC 2 cut(s) 658, 688
Bsp143I GATC 9 cut(s) 6, 29, 390, 537, 639, 854, 883, 1012, 1036
Bsp1720I GCTNAGC 1 cut(s) 831
Bsp19I CCATGG 1 cut(s) 15
BspACI CCGC 2 cut(s) 159, 250
BspANI GGCC 3 cut(s) 20, 47, 963
BspCNI CTCAG 4 cut(s) 97, 601, 823, 1137
BspDI ATCGAT 1 cut(s) 447
BspHI TCATGA 1 cut(s) 393
BspLI GGNNCC 2 cut(s) 492, 1038
BspMAI CTGCAG 1 cut(s) 801
BspPI GGATC 4 cut(s) 398, 862, 1031, 1044
BspT104I TTCGAA 1 cut(s) 518
BspTNI GGTCTC 2 cut(s) 605, 1131
BsrI ACTGG 1 cut(s) 581
BssECI CCNNGG 4 cut(s) 15, 147, 189, 850
BssMI GATC 9 cut(s) 6, 29, 390, 537, 639, 854, 883, 1012, 1036
BssT1I CCWWGG 2 cut(s) 15, 189
Bst2UI CCWGG 2 cut(s) 149, 852
Bst4CI ACNGT 1 cut(s) 544
BstAPI GCANNNNNTGC 1 cut(s) 101
BstBI TTCGAA 1 cut(s) 518
BstC8I GCNNGC 1 cut(s) 792
BstDEI CTNAG 4 cut(s) 105, 609, 831, 1124
BstDSI CCRYGG 1 cut(s) 15
BstF5I GGATG 3 cut(s) 219, 438, 1045
BstHHI GCGC 1 cut(s) 796
BstKTI GATC 9 cut(s) 9, 32, 393, 540, 642, 857, 886, 1015, 1039
BstMAI GTCTC 4 cut(s) 455, 605, 1089, 1131
BstMBI GATC 9 cut(s) 6, 29, 390, 537, 639, 854, 883, 1012, 1036
BstMCI CGRYCG 1 cut(s) 1015
BstMWI GCNNNNNNNGC 5 cut(s) 101, 335, 787, 796, 941
BstNI CCWGG 2 cut(s) 149, 852
BstNSI RCATGY 1 cut(s) 1028
BstSCI CCNGG 3 cut(s) 147, 245, 850
BstSFI CTRYAG 2 cut(s) 339, 797
BstV1I GCAGC 3 cut(s) 88, 783, 1045
BstV2I GAAGAC 1 cut(s) 746
BstX2I RGATCY 2 cut(s) 29, 1036
BstXI CCANNNNNNTGG 1 cut(s) 230
BstYI RGATCY 2 cut(s) 29, 1036
Bsu15I ATCGAT 1 cut(s) 447
BsuRI GGCC 3 cut(s) 20, 47, 963
BsuTUI ATCGAT 1 cut(s) 447
BtgI CCRYGG 1 cut(s) 15
BtsCI GGATG 3 cut(s) 219, 438, 1045
Cac8I GCNNGC 1 cut(s) 792
CciI TCATGA 1 cut(s) 393
CfoI GCGC 1 cut(s) 796
Cfr13I GGNCC 5 cut(s) 491, 713, 895, 989, 1017
ClaI ATCGAT 1 cut(s) 447
Csp6I GTAC 3 cut(s) 511, 802, 1008
CviAII CATG 4 cut(s) 16, 394, 984, 1025
CviQI GTAC 3 cut(s) 511, 802, 1008
DdeI CTNAG 4 cut(s) 105, 609, 831, 1124
DpnI GATC 9 cut(s) 8, 31, 392, 539, 641, 856, 885, 1014, 1038
DpnII GATC 9 cut(s) 6, 29, 390, 537, 639, 854, 883, 1012, 1036
EaeI YGGCCR 2 cut(s) 18, 961
Eco130I CCWWGG 2 cut(s) 15, 189
Eco24I GRGCYC 1 cut(s) 658
Eco31I GGTCTC 2 cut(s) 605, 1131
Eco32I GATATC 1 cut(s) 439
Eco47I GGWCC 5 cut(s) 491, 713, 895, 989, 1017
Eco57I CTGAAG 2 cut(s) 360, 634
EcoRII CCWGG 2 cut(s) 147, 850
EcoRV GATATC 1 cut(s) 439
EcoT14I CCWWGG 2 cut(s) 15, 189
EcoT22I ATGCAT 1 cut(s) 625
EcoT38I GRGCYC 1 cut(s) 658
ErhI CCWWGG 2 cut(s) 15, 189
FaeI CATG 4 cut(s) 19, 397, 987, 1028
FalI AAGNNNNNCTT 2 cut(s) 928, 960
FaqI GGGAC 1 cut(s) 312
FatI CATG 4 cut(s) 15, 393, 983, 1024
FauNDI CATATG 1 cut(s) 97
FbaI TGATCA 1 cut(s) 639
FblI GTMKAC 1 cut(s) 179
Fnu4HI GCNGC 3 cut(s) 102, 797, 1059
FokI GGATG 3 cut(s) 226, 445, 1032
FriOI GRGCYC 1 cut(s) 658
Fsp4HI GCNGC 3 cut(s) 102, 797, 1059
FspBI CTAG 2 cut(s) 666, 887
GlaI GCGC 1 cut(s) 795
GluI GCNGC 3 cut(s) 102, 797, 1059
GsaI CCCAGC 1 cut(s) 122
GsuI CTGGAG 1 cut(s) 252
HaeIII GGCC 3 cut(s) 20, 47, 963
HapII CCGG 1 cut(s) 246
HhaI GCGC 1 cut(s) 796
Hin1II CATG 4 cut(s) 19, 397, 987, 1028
Hin6I GCGC 1 cut(s) 794
HinP1I GCGC 1 cut(s) 794
HincII GTYRAC 1 cut(s) 180
HindII GTYRAC 1 cut(s) 180
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 3 cut(s) 61, 143, 1120
HpaII CCGG 1 cut(s) 246
HphI GGTGA 5 cut(s) 152, 247, 287, 299, 376
Hpy166II GTNNAC 3 cut(s) 180, 255, 1008
Hpy188I TCNGA 4 cut(s) 564, 610, 690, 895
Hpy188III TCNNGA 5 cut(s) 231, 378, 394, 535, 1136
Hpy8I GTNNAC 3 cut(s) 180, 255, 1008
HpyAV CCTTC 2 cut(s) 107, 811
HpyCH4III ACNGT 1 cut(s) 544
HpyCH4V TGCA 7 cut(s) 314, 401, 623, 726, 799, 872, 971
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 335, 787, 796, 941
HpyF3I CTNAG 4 cut(s) 105, 609, 831, 1124
Hsp92II CATG 4 cut(s) 19, 397, 987, 1028
HspAI GCGC 1 cut(s) 794
Ksp22I TGATCA 1 cut(s) 639
Kzo9I GATC 9 cut(s) 6, 29, 390, 537, 639, 854, 883, 1012, 1036
LmnI GCTCC 5 cut(s) 122, 334, 383, 548, 596
Lsp1109I GCAGC 3 cut(s) 88, 783, 1045
LweI GCATC 1 cut(s) 610
MaeI CTAG 2 cut(s) 666, 887
MalI GATC 9 cut(s) 8, 31, 392, 539, 641, 856, 885, 1014, 1038
MboI GATC 9 cut(s) 6, 29, 390, 537, 639, 854, 883, 1012, 1036
MboII GAAGA 3 cut(s) 302, 420, 751
MfeI CAATTG 1 cut(s) 677
MflI RGATCY 2 cut(s) 29, 1036
MhlI GDGCHC 2 cut(s) 658, 688
MlsI TGGCCA 2 cut(s) 20, 963
MluCI AATT 8 cut(s) 282, 293, 367, 568, 603, 677, 692, 1082
MluNI TGGCCA 2 cut(s) 20, 963
MmeI TCCRAC 4 cut(s) 526, 691, 873, 1016
MnlI CCTC 4 cut(s) 227, 374, 857, 908
Mox20I TGGCCA 2 cut(s) 20, 963
Mph1103I ATGCAT 1 cut(s) 625
MscI TGGCCA 2 cut(s) 20, 963
MseI TTAA 2 cut(s) 11, 1144
MslI CAYNNNNRTG 1 cut(s) 1050
Msp20I TGGCCA 2 cut(s) 20, 963
MspI CCGG 1 cut(s) 246
MspR9I CCNGG 3 cut(s) 149, 247, 852
MunI CAATTG 1 cut(s) 677
Mva1269I GAATGC 2 cut(s) 403, 623
MvaI CCWGG 2 cut(s) 149, 852
MwoI GCNNNNNNNGC 5 cut(s) 101, 335, 787, 796, 941
NciI CCSGG 1 cut(s) 247
NcoI CCATGG 1 cut(s) 15
NdeI CATATG 1 cut(s) 97
NdeII GATC 9 cut(s) 6, 29, 390, 537, 639, 854, 883, 1012, 1036
NlaIII CATG 4 cut(s) 19, 397, 987, 1028
NlaIV GGNNCC 2 cut(s) 492, 1038
NsiI ATGCAT 1 cut(s) 625
NspI RCATGY 1 cut(s) 1028
NspV TTCGAA 1 cut(s) 518
PagI TCATGA 1 cut(s) 393
PctI GAATGC 2 cut(s) 403, 623
PfeI GAWTC 3 cut(s) 61, 143, 1120
PflFI GACNNNGTC 1 cut(s) 184
PflMI CCANNNNNTGG 1 cut(s) 772
PkrI GCNGC 3 cut(s) 103, 798, 1060
Ple19I CGATCG 1 cut(s) 1015
Psp6I CCWGG 2 cut(s) 147, 850
PspFI CCCAGC 1 cut(s) 118
PspGI CCWGG 2 cut(s) 147, 850
PspN4I GGNNCC 2 cut(s) 492, 1038
PspPI GGNCC 5 cut(s) 491, 713, 895, 989, 1017
PstI CTGCAG 1 cut(s) 801
PsuI RGATCY 2 cut(s) 29, 1036
PsyI GACNNNGTC 1 cut(s) 184
PvuI CGATCG 1 cut(s) 1015
RsaI GTAC 3 cut(s) 512, 803, 1009
RsaNI GTAC 3 cut(s) 511, 802, 1008
RseI CAYNNNNRTG 1 cut(s) 1050
SalI GTCGAC 1 cut(s) 178
SaqAI TTAA 2 cut(s) 11, 1144
SatI GCNGC 3 cut(s) 102, 797, 1059
Sau3AI GATC 9 cut(s) 6, 29, 390, 537, 639, 854, 883, 1012, 1036
Sau96I GGNCC 5 cut(s) 491, 713, 895, 989, 1017
ScrFI CCNGG 3 cut(s) 149, 247, 852
SduI GDGCHC 2 cut(s) 658, 688
SfaNI GCATC 1 cut(s) 610
SfcI CTRYAG 2 cut(s) 339, 797
SfuI TTCGAA 1 cut(s) 518
SinI GGWCC 5 cut(s) 491, 713, 895, 989, 1017
SmiMI CAYNNNNRTG 1 cut(s) 1050
Sse9I AATT 8 cut(s) 282, 293, 367, 568, 603, 677, 692, 1082
SsiI CCGC 2 cut(s) 159, 250
SspI AATATT 3 cut(s) 199, 354, 414
SspMI CTAG 2 cut(s) 666, 887
StyD4I CCNGG 3 cut(s) 147, 245, 850
StyI CCWWGG 2 cut(s) 15, 189
TaaI ACNGT 1 cut(s) 544
TaqI TCGA 5 cut(s) 179, 447, 518, 536, 1135
TaqII GACCGA 1 cut(s) 1005
TasI AATT 8 cut(s) 282, 293, 367, 568, 603, 677, 692, 1082
TatI WGTACW 2 cut(s) 510, 801
TfiI GAWTC 3 cut(s) 61, 143, 1120
Tru1I TTAA 2 cut(s) 11, 1144
Tru9I TTAA 2 cut(s) 11, 1144
TseI GCWGC 3 cut(s) 101, 796, 1058
TspDTI ATGAA 3 cut(s) 410, 616, 1133
Tth111I GACNNNGTC 1 cut(s) 184
Van91I CCANNNNNTGG 1 cut(s) 772
VpaK11BI GGWCC 5 cut(s) 491, 713, 895, 989, 1017
XapI RAATTY 2 cut(s) 603, 692
XceI RCATGY 1 cut(s) 1028
XmiI GTMKAC 1 cut(s) 179
XspI CTAG 2 cut(s) 666, 887
Zsp2I ATGCAT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.