MD15G1275100.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015376
Physical Location & Seq
Forward (+)
24155419 .. 24158224
2806 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1275100.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGAGTATAATTCTTGCACTGCGGCCTGCGTAGGGTTTGTTCTTTTGCTCGTTTTTAATCAGAGTTATTTGGGAAATGCTGCTAATCAAGGTCAAGGGTTAGGAGCTTCTTTTATTTTTGGTGATTCTCTGGTTGATGCTGGAAACAATAATTATTTACCAACGTTGTCCAAGGCGAATATGGTTCCAAACGGGATCGATTTTAAAGCATCTGGAGGAAAACCTACCGGAAGGTTTACCAATGGTAGAACCATTGGTGATATAGTTGGAGAAGAATTGGGGCAACCAAACTATGCACTCCCATATTTGTCACCAAATGCCACAGGAAAAGCCCTATTGGTTGGGGTGAATTATGCATCAGGAGGAGCAGGGATTTTGAATGCAACAGGAAGAATATTTATTAATAGGGTGGGAATGGATATCCAAGTCGATTTTTTCAACCAGACAAGGAAGCAGATTGACAAGGTATTGGGCCCATCAAAGGCAAAAGAGTACATTATGAAAAGATCCATTTTTTCAATCACAGCTGGGTCCAATGACTTTTTGAACAATTATCTCCTACCAGTCCTCTCCATTGGAACAAGAATTTCCCAGAGCCCAGACGCTTTTATTGATGATTTGATCTCTCACTTCAGAGTCCAACTTACTAGACTTTATCAATTGGATGCTCGAAAATTTGTGATTGGAAGTGTTGGACCTATTGGCTGCATACCATACCAAAAGACCATAAATCAACTGAATCCAGACCAGTGTGTGGAGTTGCCAAATAAGCTAGCTCTTCAGTACAATGGCCGGTTGAAGGGCTTGCTTGCTGAACTGAATAAGAACCTTCCAGGTTCAACATTTGTTTATGCAAACGTGTATGATCTAGTGATGGACGTCATTACGAATTATGAAAAATATGGATTTACAACAGCAAGTGTAGCATGCTGTGGAAATGGTGGCCAATACGCAGGTATAATTCCATGTGGTCCACAATCCAGTCTCTGCGAGGATCGGTTGATGCATGTTTTCTGGGATCCCTACCATCCAAGTGATTCTGCCAACCTTATAATCGCCAAAAAATTGCTCGATGGAGACACAAAAATAATTTATCCAATGAATCTCAGACAACTTCGAGACCATGGATTAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

379

Amino Acids

41.05

Weight (kDa)

8.31

Isoelectric Point (pI)

26.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 356 1.7e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23540
fragaria_vesca FvH4_1g14780
malus_domestica MD02G1161000.v1.1 MD15G1275100.v1.1
prunus_persica Prupe.7G142600_v2.0.a1
pyrus_communis pycom02g12710 pycom15g23820
rosa_chinensis RchiOBHm_Chr2g0103741
rosa_laevigata RLG00000017253
rosa_multiflora Rmu_sc0001852.1_g000002
rosa_roxburghii Rroxscaffold_2G00139440
rosa_rugosa Rorug02G0117000
rosa_samantha Rh2AG165800 Rh2BG173100 Rh2CG171800
rosa_wichuraiana Rw2G012990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1052
AatII GACGTC 1 cut(s) 882
Acc36I ACCTGC 1 cut(s) 944
AccB7I CCANNNNNTGG 1 cut(s) 754
AciI CCGC 1 cut(s) 23
AclI AACGTT 1 cut(s) 164
AclWI GGATC 5 cut(s) 203, 501, 1002, 1013, 1026
AcoI YGGCCR 2 cut(s) 790, 943
AcsI RAATTY 2 cut(s) 585, 674
AcuI CTGAAG 2 cut(s) 616, 764
AcyI GRCGYC 1 cut(s) 879
AfaI GTAC 2 cut(s) 494, 785
AfiI CCNNNNNNNGG 4 cut(s) 33, 481, 754, 799
AflIII ACRYGT 1 cut(s) 858
AgsI TTSAA 6 cut(s) 379, 439, 519, 547, 799, 840
AjnI CCWGG 1 cut(s) 832
AluBI AGCT 4 cut(s) 107, 527, 772, 776
AluI AGCT 4 cut(s) 107, 527, 772, 776
Alw26I GTCTC 3 cut(s) 989, 1071, 1113
AlwI GGATC 5 cut(s) 203, 501, 1002, 1013, 1026
AlwNI CAGNNNCTG 1 cut(s) 987
AoxI GGCC 4 cut(s) 24, 472, 790, 943
ApaI GGGCCC 1 cut(s) 476
ApeKI GCWGC 2 cut(s) 80, 705
ApoI RAATTY 2 cut(s) 585, 674
AseI ATTAAT 1 cut(s) 402
AspS9I GGNCC 5 cut(s) 472, 473, 531, 695, 971
AsuHPI GGTGA 4 cut(s) 134, 269, 303, 358
AsuNHI GCTAGC 1 cut(s) 772
AvaII GGWCC 3 cut(s) 531, 695, 971
BaeGI GKGCMC 1 cut(s) 476
BalI TGGCCA 1 cut(s) 945
BamHI GGATCC 1 cut(s) 1018
BanII GRGCYC 2 cut(s) 476, 599
BbvI GCAGC 2 cut(s) 67, 692
BccI CCATC 4 cut(s) 484, 868, 1035, 1067
BciT130I CCWGG 1 cut(s) 834
BcoDI GTCTC 3 cut(s) 989, 1071, 1113
BfaI CTAG 3 cut(s) 648, 773, 869
BfuAI ACCTGC 1 cut(s) 944
BisI GCNGC 3 cut(s) 24, 81, 706
BlsI GCNGC 3 cut(s) 25, 82, 707
Bme1390I CCNGG 1 cut(s) 834
Bme18I GGWCC 3 cut(s) 531, 695, 971
BmgT120I GGNCC 5 cut(s) 472, 473, 531, 695, 971
BmiI GGNNCC 4 cut(s) 186, 474, 532, 1020
BmrFI CCNGG 1 cut(s) 834
BmsI GCATC 5 cut(s) 127, 218, 365, 655, 993
BmtI GCTAGC 1 cut(s) 776
BpmI CTGGAG 1 cut(s) 234
Bsa29I ATCGAT 1 cut(s) 198
BsaHI GRCGYC 1 cut(s) 879
BsaI GGTCTC 1 cut(s) 1113
BsaJI CCNNGG 2 cut(s) 171, 1123
BsaWI WCCGGW 1 cut(s) 227
Bsc4I CCNNNNNNNGG 4 cut(s) 33, 481, 754, 799
Bse118I RCCGGY 1 cut(s) 792
Bse1I ACTGG 3 cut(s) 563, 748, 981
BseBI CCWGG 1 cut(s) 834
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 2 cut(s) 171, 1123
BseGI GGATG 2 cut(s) 670, 1027
BseLI CCNNNNNNNGG 4 cut(s) 33, 481, 754, 799
BseMII CTCAG 1 cut(s) 1120
BseNI ACTGG 3 cut(s) 563, 748, 981
BseRI GAGGAG 1 cut(s) 378
BseSI GKGCMC 1 cut(s) 476
BseXI GCAGC 2 cut(s) 67, 692
BseYI CCCAGC 1 cut(s) 527
BshFI GGCC 4 cut(s) 26, 474, 792, 945
BshVI ATCGAT 1 cut(s) 198
BsiSI CCGG 2 cut(s) 228, 793
BslI CCNNNNNNNGG 4 cut(s) 33, 481, 754, 799
BsmAI GTCTC 3 cut(s) 989, 1071, 1113
BsmI GAATGC 1 cut(s) 385
BsnI GGCC 4 cut(s) 26, 474, 792, 945
Bso31I GGTCTC 1 cut(s) 1113
Bsp120I GGGCCC 1 cut(s) 472
Bsp1286I GDGCHC 2 cut(s) 476, 599
Bsp143I GATC 6 cut(s) 195, 506, 621, 865, 994, 1018
Bsp19I CCATGG 1 cut(s) 1123
BspACI CCGC 1 cut(s) 23
BspANI GGCC 4 cut(s) 26, 474, 792, 945
BspCNI CTCAG 1 cut(s) 1119
BspDI ATCGAT 1 cut(s) 198
BspLI GGNNCC 4 cut(s) 186, 474, 532, 1020
BspMI ACCTGC 1 cut(s) 944
BspOI GCTAGC 1 cut(s) 776
BspPI GGATC 5 cut(s) 203, 501, 1002, 1013, 1026
BspQI GCTCTTC 1 cut(s) 783
BspTNI GGTCTC 1 cut(s) 1113
BsrFI RCCGGY 1 cut(s) 792
BsrI ACTGG 3 cut(s) 563, 748, 981
BssAI RCCGGY 1 cut(s) 792
BssECI CCNNGG 2 cut(s) 171, 1123
BssMI GATC 6 cut(s) 195, 506, 621, 865, 994, 1018
BssNI GRCGYC 1 cut(s) 879
BssT1I CCWWGG 2 cut(s) 171, 1123
Bst2UI CCWGG 1 cut(s) 834
Bst6I CTCTTC 1 cut(s) 783
BstACI GRCGYC 1 cut(s) 879
BstC8I GCNNGC 5 cut(s) 28, 774, 806, 810, 928
BstDEI CTNAG 1 cut(s) 1106
BstDSI CCRYGG 1 cut(s) 1123
BstF5I GGATG 2 cut(s) 670, 1027
BstKTI GATC 6 cut(s) 198, 509, 624, 868, 997, 1021
BstMAI GTCTC 3 cut(s) 989, 1071, 1113
BstMBI GATC 6 cut(s) 195, 506, 621, 865, 994, 1018
BstMWI GCNNNNNNNGC 2 cut(s) 769, 923
BstNI CCWGG 1 cut(s) 834
BstNSI RCATGY 2 cut(s) 930, 1010
BstSCI CCNGG 1 cut(s) 832
BstSLI GKGCMC 1 cut(s) 476
BstV1I GCAGC 2 cut(s) 67, 692
BstX2I RGATCY 2 cut(s) 506, 1018
BstYI RGATCY 2 cut(s) 506, 1018
Bsu15I ATCGAT 1 cut(s) 198
BsuRI GGCC 4 cut(s) 26, 474, 792, 945
BsuTUI ATCGAT 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 1123
BtsCI GGATG 2 cut(s) 670, 1027
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 2 cut(s) 18, 755
BveI ACCTGC 1 cut(s) 944
Cac8I GCNNGC 5 cut(s) 28, 774, 806, 810, 928
CaiI CAGNNNCTG 1 cut(s) 987
Cfr10I RCCGGY 1 cut(s) 792
Cfr13I GGNCC 5 cut(s) 472, 473, 531, 695, 971
ClaI ATCGAT 1 cut(s) 198
CseI GACGC 1 cut(s) 611
Csp6I GTAC 2 cut(s) 493, 784
CviAII CATG 4 cut(s) 927, 966, 1007, 1124
CviQI GTAC 2 cut(s) 493, 784
DdeI CTNAG 1 cut(s) 1106
DpnI GATC 6 cut(s) 197, 508, 623, 867, 996, 1020
DpnII GATC 6 cut(s) 195, 506, 621, 865, 994, 1018
DraI TTTAAA 1 cut(s) 205
EaeI YGGCCR 2 cut(s) 790, 943
Eam1104I CTCTTC 1 cut(s) 783
EarI CTCTTC 1 cut(s) 783
Eco130I CCWWGG 2 cut(s) 171, 1123
Eco24I GRGCYC 2 cut(s) 476, 599
Eco31I GGTCTC 1 cut(s) 1113
Eco32I GATATC 1 cut(s) 421
Eco47I GGWCC 3 cut(s) 531, 695, 971
Eco57I CTGAAG 2 cut(s) 616, 764
EcoRII CCWGG 1 cut(s) 832
EcoRV GATATC 1 cut(s) 421
EcoT14I CCWWGG 2 cut(s) 171, 1123
EcoT22I ATGCAT 2 cut(s) 358, 1008
EcoT38I GRGCYC 2 cut(s) 476, 599
ErhI CCWWGG 2 cut(s) 171, 1123
FaeI CATG 4 cut(s) 930, 969, 1010, 1127
FatI CATG 4 cut(s) 926, 965, 1006, 1123
Fnu4HI GCNGC 3 cut(s) 24, 81, 706
FokI GGATG 2 cut(s) 677, 1014
FriOI GRGCYC 2 cut(s) 476, 599
Fsp4HI GCNGC 3 cut(s) 24, 81, 706
FspBI CTAG 3 cut(s) 648, 773, 869
GluI GCNGC 3 cut(s) 24, 81, 706
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 234
HaeIII GGCC 4 cut(s) 26, 474, 792, 945
HapII CCGG 2 cut(s) 228, 793
HgaI GACGC 1 cut(s) 611
Hin1I GRCGYC 1 cut(s) 879
Hin1II CATG 4 cut(s) 930, 969, 1010, 1127
HinfI GANTC 5 cut(s) 125, 636, 739, 1037, 1102
HpaII CCGG 2 cut(s) 228, 793
HphI GGTGA 4 cut(s) 134, 269, 303, 358
Hpy166II GTNNAC 2 cut(s) 237, 974
Hpy188I TCNGA 3 cut(s) 63, 635, 1109
Hpy188III TCNNGA 4 cut(s) 213, 360, 743, 1118
Hpy8I GTNNAC 2 cut(s) 237, 974
HpyAV CCTTC 3 cut(s) 225, 793, 839
HpyCH4IV ACGT 3 cut(s) 164, 858, 879
HpyCH4V TGCA 7 cut(s) 18, 296, 356, 383, 708, 854, 1006
HpyF10VI GCNNNNNNNGC 2 cut(s) 769, 923
HpyF3I CTNAG 1 cut(s) 1106
HpySE526I ACGT 3 cut(s) 164, 858, 879
Hsp92I GRCGYC 1 cut(s) 879
Hsp92II CATG 4 cut(s) 930, 969, 1010, 1127
Kzo9I GATC 6 cut(s) 195, 506, 621, 865, 994, 1018
LguI GCTCTTC 1 cut(s) 783
LmnI GCTCC 2 cut(s) 104, 365
Lsp1109I GCAGC 2 cut(s) 67, 692
LweI GCATC 5 cut(s) 127, 218, 365, 655, 993
MaeI CTAG 3 cut(s) 648, 773, 869
MaeII ACGT 3 cut(s) 164, 858, 879
MaeIII GTNAC 1 cut(s) 309
MalI GATC 6 cut(s) 197, 508, 623, 867, 996, 1020
MboI GATC 6 cut(s) 195, 506, 621, 865, 994, 1018
MboII GAAGA 3 cut(s) 284, 402, 770
MfeI CAATTG 1 cut(s) 659
MflI RGATCY 2 cut(s) 506, 1018
MhlI GDGCHC 2 cut(s) 476, 599
MlsI TGGCCA 1 cut(s) 945
MluNI TGGCCA 1 cut(s) 945
MlyI GAGTC 1 cut(s) 645
MmeI TCCRAC 3 cut(s) 247, 664, 673
MnlI CCTC 4 cut(s) 209, 356, 578, 985
Mox20I TGGCCA 1 cut(s) 945
Mph1103I ATGCAT 2 cut(s) 358, 1008
MscI TGGCCA 1 cut(s) 945
MseI TTAA 3 cut(s) 57, 204, 402
MslI CAYNNNNRTG 1 cut(s) 1032
Msp20I TGGCCA 1 cut(s) 945
MspA1I CMGCKG 1 cut(s) 527
MspI CCGG 2 cut(s) 228, 793
MspR9I CCNGG 1 cut(s) 834
MunI CAATTG 1 cut(s) 659
Mva1269I GAATGC 1 cut(s) 385
MvaI CCWGG 1 cut(s) 834
MwoI GCNNNNNNNGC 2 cut(s) 769, 923
NcoI CCATGG 1 cut(s) 1123
NdeII GATC 6 cut(s) 195, 506, 621, 865, 994, 1018
NheI GCTAGC 1 cut(s) 772
NlaIII CATG 4 cut(s) 930, 969, 1010, 1127
NlaIV GGNNCC 4 cut(s) 186, 474, 532, 1020
NmuCI GTSAC 1 cut(s) 309
NsiI ATGCAT 2 cut(s) 358, 1008
NspI RCATGY 2 cut(s) 930, 1010
PaeI GCATGC 1 cut(s) 930
PciSI GCTCTTC 1 cut(s) 783
PctI GAATGC 1 cut(s) 385
PfeI GAWTC 4 cut(s) 125, 739, 1037, 1102
PflMI CCANNNNNTGG 1 cut(s) 754
PkrI GCNGC 3 cut(s) 25, 82, 707
PleI GAGTC 1 cut(s) 644
PpsI GAGTC 1 cut(s) 644
PshBI ATTAAT 1 cut(s) 402
PsiI TTATAA 1 cut(s) 1052
Psp1406I AACGTT 1 cut(s) 164
Psp6I CCWGG 1 cut(s) 832
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 1 cut(s) 832
PspN4I GGNNCC 4 cut(s) 186, 474, 532, 1020
PspOMI GGGCCC 1 cut(s) 472
PspPI GGNCC 5 cut(s) 472, 473, 531, 695, 971
PsrI GAACNNNNNNTAC 2 cut(s) 24, 56
PstNI CAGNNNCTG 1 cut(s) 987
PsuI RGATCY 2 cut(s) 506, 1018
PvuII CAGCTG 1 cut(s) 527
RsaI GTAC 2 cut(s) 494, 785
RsaNI GTAC 2 cut(s) 493, 784
RseI CAYNNNNRTG 1 cut(s) 1032
SapI GCTCTTC 1 cut(s) 783
SaqAI TTAA 3 cut(s) 57, 204, 402
SatI GCNGC 3 cut(s) 24, 81, 706
Sau3AI GATC 6 cut(s) 195, 506, 621, 865, 994, 1018
Sau96I GGNCC 5 cut(s) 472, 473, 531, 695, 971
SchI GAGTC 1 cut(s) 645
ScrFI CCNGG 1 cut(s) 834
SduI GDGCHC 2 cut(s) 476, 599
SfaNI GCATC 5 cut(s) 127, 218, 365, 655, 993
SinI GGWCC 3 cut(s) 531, 695, 971
SmiMI CAYNNNNRTG 1 cut(s) 1032
SphI GCATGC 1 cut(s) 930
SsiI CCGC 1 cut(s) 23
SspI AATATT 1 cut(s) 396
SspMI CTAG 3 cut(s) 648, 773, 869
StyD4I CCNGG 1 cut(s) 832
StyI CCWWGG 2 cut(s) 171, 1123
TaiI ACGT 3 cut(s) 167, 861, 882
TaqI TCGA 5 cut(s) 198, 429, 670, 1071, 1117
TatI WGTACW 2 cut(s) 492, 783
TauI GCSGC 1 cut(s) 26
TfiI GAWTC 4 cut(s) 125, 739, 1037, 1102
Tru1I TTAA 3 cut(s) 57, 204, 402
Tru9I TTAA 3 cut(s) 57, 204, 402
TscAI CASTG 2 cut(s) 25, 755
TseFI GTSAC 1 cut(s) 309
TseI GCWGC 2 cut(s) 80, 705
Tsp45I GTSAC 1 cut(s) 309
TspDTI ATGAA 3 cut(s) 515, 909, 1115
TspRI CASTG 2 cut(s) 25, 755
Van91I CCANNNNNTGG 1 cut(s) 754
VpaK11BI GGWCC 3 cut(s) 531, 695, 971
VspI ATTAAT 1 cut(s) 402
XapI RAATTY 2 cut(s) 585, 674
XceI RCATGY 2 cut(s) 930, 1010
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XspI CTAG 3 cut(s) 648, 773, 869
ZraI GACGTC 1 cut(s) 880
Zsp2I ATGCAT 2 cut(s) 358, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.