Rroxscaffold_2G00139440

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015376
Physical Location & Seq
Reverse (-)
77836040 .. 77838376
2337 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139440.1

Sequence Viewer

Length: 1143 bp
ATGATCTTAACCATGGCCATAATAAGATCTTATATCACTCTGGCCTTTGTAGGCTTGATTCTTTTGCTCATCAACAACCTGAGCTTTTTGGCATATGCTGCTGAGAGTGAAGGGCTGGGAGCTTCTTTTATCTTTGGTGATTCCCTGGTTGATTGTGGAAACAATAACTACTTGGCGACATTGTCCAAGGCAAATATTCGTCCTAATGGGATGGATTTCCAGCCATCTGGAGGAAATCCCACCGGGTGGTTCACCAATGGTAGAACCATTGGTGATATAATTGGTGAAGAATTGGGACAACCAAATTATGCAGTTCCATTTTTAGCTCCTAACGCTACAGGGAAAGCAATATTGTATGGGGTGAATTATGCTTCAGGAGGAGCAGGGATCATGAATGCAACAGGAAGAATATTTGTAAATAGGGTGGGGATGGATATCCAAATCGATTATTTCAACGAGACAAGGAAGCAGATTGATAAGCTACTGGGTCCATCAAAGGCAAAAGAGTACATTTCGAAAAGGTCTATTTTCTCGATCACAGTTGGAGCAAATGACTTTCTGAACAATTACCTACTACCAGTCCTATCCATTGGAGCAAGAATTTCTCAGACCCCAGATGCATTCATTGATGATTTGATCACTCACTTCAGGGCTCAACTTACTAGACTTTATCAATTGGGTGCTCGGAAATTTGTGATAGGAAATGTTGGACCAATAGGTTGCATACCTTACCAGAAGACTATCAATCAGCTCGACCAAGACCAATGTGTGGAGTTGCCTAATAAGCTTGCGCTGCAGTACAATGGCAGATTGAAGGACTTGGTTGCTGAGCTGAATGATAACCTCCCCGGATCAACATTTATTTATGCAAATGTGTATGATCTAGTGTCGGACCTCATTACCAACTACGAAAAATATGGATTTTCAACAGCAAGTAGAGCTTGTTGTGGAAATGGTGGCCAATATGCAGGAATAGTTCCATGTGGTCCAACATCAAGTCTGTGTACCGATCGGTCCAAGCATGTATTCTGGGATCCCTACCATCCAAGTGAAGCTGCCAACATCATACTCGCCAAAAAATTGCTTGATGGAGACACAAGAGTTATTTCTCCAATGAATCTCAGGCAACTTCGAGACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

380

Amino Acids

41.52

Weight (kDa)

6.88

Isoelectric Point (pI)

30.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 43 - 361 7.2e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23540
fragaria_vesca FvH4_1g14780
malus_domestica MD02G1161000.v1.1 MD15G1275100.v1.1
prunus_persica Prupe.7G142600_v2.0.a1
pyrus_communis pycom02g12710 pycom15g23820
rosa_chinensis RchiOBHm_Chr2g0103741
rosa_laevigata RLG00000017253
rosa_multiflora Rmu_sc0001852.1_g000002
rosa_roxburghii Rroxscaffold_2G00139440
rosa_rugosa Rorug02G0117000
rosa_samantha Rh2AG165800 Rh2BG173100 Rh2CG171800
rosa_wichuraiana Rw2G012990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 246, 769
AclWI GGATC 4 cut(s) 395, 859, 1028, 1041
AcoI YGGCCR 2 cut(s) 15, 958
AcsI RAATTY 2 cut(s) 600, 689
AcuI CTGAAG 2 cut(s) 357, 631
AdeI CACNNNGTG 1 cut(s) 246
AfaI GTAC 3 cut(s) 509, 800, 1006
AfiI CCNNNNNNNGG 3 cut(s) 230, 246, 769
AgsI TTSAA 3 cut(s) 454, 814, 927
AjnI CCWGG 1 cut(s) 144
AjuI GAANNNNNNNTTGG 2 cut(s) 1010, 1042
AluBI AGCT 9 cut(s) 84, 122, 326, 481, 751, 787, 832, 941, 1055
AluI AGCT 9 cut(s) 84, 122, 326, 481, 751, 787, 832, 941, 1055
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 3 cut(s) 452, 1086, 1128
AlwI GGATC 4 cut(s) 395, 859, 1028, 1041
AoxI GGCC 3 cut(s) 15, 42, 958
ApeKI GCWGC 3 cut(s) 98, 793, 1055
ApoI RAATTY 2 cut(s) 600, 689
AspLEI GCGC 1 cut(s) 793
AspS9I GGNCC 5 cut(s) 488, 710, 892, 986, 1014
AsuC2I CCSGG 2 cut(s) 244, 849
AsuHPI GGTGA 5 cut(s) 149, 244, 284, 296, 373
AsuII TTCGAA 1 cut(s) 515
AvaII GGWCC 5 cut(s) 488, 710, 892, 986, 1014
BalI TGGCCA 2 cut(s) 17, 960
BamHI GGATCC 1 cut(s) 1033
BanII GRGCYC 1 cut(s) 655
BbsI GAAGAC 1 cut(s) 743
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 3 cut(s) 85, 780, 1042
BccI CCATC 6 cut(s) 205, 232, 424, 499, 1050, 1082
BciT130I CCWGG 1 cut(s) 146
BclI TGATCA 1 cut(s) 636
BcnI CCSGG 2 cut(s) 244, 849
BcoDI GTCTC 3 cut(s) 452, 1086, 1128
BfaI CTAG 2 cut(s) 663, 884
BfmI CTRYAG 2 cut(s) 336, 794
BglII AGATCT 1 cut(s) 26
BisI GCNGC 3 cut(s) 99, 794, 1056
BlpI GCTNAGC 1 cut(s) 828
BlsI GCNGC 3 cut(s) 100, 795, 1057
Bme1390I CCNGG 3 cut(s) 146, 244, 849
Bme18I GGWCC 5 cut(s) 488, 710, 892, 986, 1014
BmgT120I GGNCC 5 cut(s) 488, 710, 892, 986, 1014
BmiI GGNNCC 2 cut(s) 489, 1035
BmrFI CCNGG 3 cut(s) 146, 244, 849
BmrI ACTGGG 1 cut(s) 494
BmsI GCATC 1 cut(s) 607
BmuI ACTGGG 1 cut(s) 494
BpiI GAAGAC 1 cut(s) 743
BpmI CTGGAG 1 cut(s) 249
Bpu10I CCTNAGC 1 cut(s) 80
Bpu1102I GCTNAGC 1 cut(s) 828
Bpu14I TTCGAA 1 cut(s) 515
BpuMI CCSGG 2 cut(s) 244, 849
Bsa29I ATCGAT 1 cut(s) 444
BsaBI GATNNNNATC 1 cut(s) 434
BsaI GGTCTC 1 cut(s) 1128
BsaJI CCNNGG 4 cut(s) 12, 144, 186, 847
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 246, 769
Bse1I ACTGG 2 cut(s) 489, 578
Bse8I GATNNNNATC 1 cut(s) 434
BseBI CCWGG 1 cut(s) 146
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 4 cut(s) 12, 144, 186, 847
BseGI GGATG 3 cut(s) 216, 435, 1042
BseJI GATNNNNATC 1 cut(s) 434
BseLI CCNNNNNNNGG 3 cut(s) 230, 246, 769
BseMII CTCAG 5 cut(s) 71, 93, 620, 819, 1135
BseNI ACTGG 2 cut(s) 489, 578
BseRI GAGGAG 1 cut(s) 393
BseXI GCAGC 3 cut(s) 85, 780, 1042
BseYI CCCAGC 1 cut(s) 115
Bsh1285I CGRYCG 1 cut(s) 1012
BshFI GGCC 3 cut(s) 17, 44, 960
BshVI ATCGAT 1 cut(s) 444
BsiEI CGRYCG 1 cut(s) 1012
BsiHKAI GWGCWC 1 cut(s) 685
BsiSI CCGG 2 cut(s) 243, 849
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 3 cut(s) 230, 246, 769
BsmAI GTCTC 3 cut(s) 452, 1086, 1128
BsmFI GGGAC 1 cut(s) 309
BsmI GAATGC 2 cut(s) 400, 620
BsnI GGCC 3 cut(s) 17, 44, 960
Bso31I GGTCTC 1 cut(s) 1128
Bsp119I TTCGAA 1 cut(s) 515
Bsp1286I GDGCHC 2 cut(s) 655, 685
Bsp143I GATC 9 cut(s) 3, 26, 387, 534, 636, 851, 880, 1009, 1033
Bsp1720I GCTNAGC 1 cut(s) 828
Bsp19I CCATGG 1 cut(s) 12
BspANI GGCC 3 cut(s) 17, 44, 960
BspCNI CTCAG 5 cut(s) 72, 94, 619, 820, 1134
BspDI ATCGAT 1 cut(s) 444
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 2 cut(s) 489, 1035
BspMAI CTGCAG 1 cut(s) 798
BspPI GGATC 4 cut(s) 395, 859, 1028, 1041
BspT104I TTCGAA 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 1128
BsrI ACTGG 2 cut(s) 489, 578
BssECI CCNNGG 4 cut(s) 12, 144, 186, 847
BssMI GATC 9 cut(s) 3, 26, 387, 534, 636, 851, 880, 1009, 1033
BssT1I CCWWGG 2 cut(s) 12, 186
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 541
BstAPI GCANNNNNTGC 1 cut(s) 98
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 1 cut(s) 789
BstDEI CTNAG 5 cut(s) 80, 102, 606, 828, 1121
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 3 cut(s) 216, 435, 1042
BstHHI GCGC 1 cut(s) 793
BstKTI GATC 9 cut(s) 6, 29, 390, 537, 639, 854, 883, 1012, 1036
BstMAI GTCTC 3 cut(s) 452, 1086, 1128
BstMBI GATC 9 cut(s) 3, 26, 387, 534, 636, 851, 880, 1009, 1033
BstMCI CGRYCG 1 cut(s) 1012
BstMWI GCNNNNNNNGC 5 cut(s) 98, 332, 784, 793, 938
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 1025
BstSCI CCNGG 3 cut(s) 144, 242, 847
BstSFI CTRYAG 2 cut(s) 336, 794
BstV1I GCAGC 3 cut(s) 85, 780, 1042
BstV2I GAAGAC 1 cut(s) 743
BstX2I RGATCY 2 cut(s) 26, 1033
BstXI CCANNNNNNTGG 1 cut(s) 227
BstYI RGATCY 2 cut(s) 26, 1033
Bsu15I ATCGAT 1 cut(s) 444
BsuRI GGCC 3 cut(s) 17, 44, 960
BsuTUI ATCGAT 1 cut(s) 444
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 3 cut(s) 216, 435, 1042
Cac8I GCNNGC 1 cut(s) 789
CciI TCATGA 1 cut(s) 390
CfoI GCGC 1 cut(s) 793
Cfr13I GGNCC 5 cut(s) 488, 710, 892, 986, 1014
ClaI ATCGAT 1 cut(s) 444
Csp6I GTAC 3 cut(s) 508, 799, 1005
CviAII CATG 4 cut(s) 13, 391, 981, 1022
CviQI GTAC 3 cut(s) 508, 799, 1005
DdeI CTNAG 5 cut(s) 80, 102, 606, 828, 1121
DpnI GATC 9 cut(s) 5, 28, 389, 536, 638, 853, 882, 1011, 1035
DpnII GATC 9 cut(s) 3, 26, 387, 534, 636, 851, 880, 1009, 1033
DraIII CACNNNGTG 1 cut(s) 246
EaeI YGGCCR 2 cut(s) 15, 958
Eco130I CCWWGG 2 cut(s) 12, 186
Eco24I GRGCYC 1 cut(s) 655
Eco31I GGTCTC 1 cut(s) 1128
Eco32I GATATC 1 cut(s) 436
Eco47I GGWCC 5 cut(s) 488, 710, 892, 986, 1014
Eco57I CTGAAG 2 cut(s) 357, 631
EcoRII CCWGG 1 cut(s) 144
EcoRV GATATC 1 cut(s) 436
EcoT14I CCWWGG 2 cut(s) 12, 186
EcoT22I ATGCAT 1 cut(s) 622
EcoT38I GRGCYC 1 cut(s) 655
ErhI CCWWGG 2 cut(s) 12, 186
FaeI CATG 4 cut(s) 16, 394, 984, 1025
FalI AAGNNNNNCTT 2 cut(s) 925, 957
FaqI GGGAC 1 cut(s) 309
FatI CATG 4 cut(s) 12, 390, 980, 1021
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 1 cut(s) 636
Fnu4HI GCNGC 3 cut(s) 99, 794, 1056
FokI GGATG 3 cut(s) 223, 442, 1029
FriOI GRGCYC 1 cut(s) 655
Fsp4HI GCNGC 3 cut(s) 99, 794, 1056
FspBI CTAG 2 cut(s) 663, 884
GlaI GCGC 1 cut(s) 792
GluI GCNGC 3 cut(s) 99, 794, 1056
GsaI CCCAGC 1 cut(s) 119
GsuI CTGGAG 1 cut(s) 249
HaeIII GGCC 3 cut(s) 17, 44, 960
HapII CCGG 2 cut(s) 243, 849
HhaI GCGC 1 cut(s) 793
Hin1II CATG 4 cut(s) 16, 394, 984, 1025
Hin6I GCGC 1 cut(s) 791
HinP1I GCGC 1 cut(s) 791
HindIII AAGCTT 1 cut(s) 785
HinfI GANTC 3 cut(s) 58, 140, 1117
HpaII CCGG 2 cut(s) 243, 849
HphI GGTGA 5 cut(s) 149, 244, 284, 296, 373
Hpy166II GTNNAC 2 cut(s) 252, 1005
Hpy188I TCNGA 4 cut(s) 561, 609, 687, 892
Hpy188III TCNNGA 5 cut(s) 228, 375, 391, 532, 1133
Hpy8I GTNNAC 2 cut(s) 252, 1005
HpyAV CCTTC 2 cut(s) 104, 808
HpyCH4III ACNGT 1 cut(s) 541
HpyCH4V TGCA 7 cut(s) 311, 398, 620, 723, 796, 869, 968
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 332, 784, 793, 938
HpyF3I CTNAG 5 cut(s) 80, 102, 606, 828, 1121
Hsp92II CATG 4 cut(s) 16, 394, 984, 1025
HspAI GCGC 1 cut(s) 791
Ksp22I TGATCA 1 cut(s) 636
Kzo9I GATC 9 cut(s) 3, 26, 387, 534, 636, 851, 880, 1009, 1033
LmnI GCTCC 5 cut(s) 119, 331, 380, 545, 593
Lsp1109I GCAGC 3 cut(s) 85, 780, 1042
LweI GCATC 1 cut(s) 607
MaeI CTAG 2 cut(s) 663, 884
MalI GATC 9 cut(s) 5, 28, 389, 536, 638, 853, 882, 1011, 1035
MboI GATC 9 cut(s) 3, 26, 387, 534, 636, 851, 880, 1009, 1033
MboII GAAGA 3 cut(s) 299, 417, 748
MfeI CAATTG 1 cut(s) 674
MflI RGATCY 2 cut(s) 26, 1033
MhlI GDGCHC 2 cut(s) 655, 685
MlsI TGGCCA 2 cut(s) 17, 960
MluCI AATT 9 cut(s) 279, 290, 304, 364, 565, 600, 674, 689, 1079
MluNI TGGCCA 2 cut(s) 17, 960
MmeI TCCRAC 4 cut(s) 523, 688, 870, 1013
MnlI CCTC 4 cut(s) 224, 371, 854, 905
Mox20I TGGCCA 2 cut(s) 17, 960
Mph1103I ATGCAT 1 cut(s) 622
MscI TGGCCA 2 cut(s) 17, 960
MseI TTAA 2 cut(s) 8, 1141
MslI CAYNNNNRTG 1 cut(s) 1047
Msp20I TGGCCA 2 cut(s) 17, 960
MspI CCGG 2 cut(s) 243, 849
MspR9I CCNGG 3 cut(s) 146, 244, 849
MunI CAATTG 1 cut(s) 674
Mva1269I GAATGC 2 cut(s) 400, 620
MvaI CCWGG 1 cut(s) 146
MwoI GCNNNNNNNGC 5 cut(s) 98, 332, 784, 793, 938
NciI CCSGG 2 cut(s) 244, 849
NcoI CCATGG 1 cut(s) 12
NdeI CATATG 1 cut(s) 94
NdeII GATC 9 cut(s) 3, 26, 387, 534, 636, 851, 880, 1009, 1033
NlaIII CATG 4 cut(s) 16, 394, 984, 1025
NlaIV GGNNCC 2 cut(s) 489, 1035
NsiI ATGCAT 1 cut(s) 622
NspI RCATGY 1 cut(s) 1025
NspV TTCGAA 1 cut(s) 515
PagI TCATGA 1 cut(s) 390
PctI GAATGC 2 cut(s) 400, 620
PfeI GAWTC 3 cut(s) 58, 140, 1117
PflFI GACNNNGTC 1 cut(s) 181
PflMI CCANNNNNTGG 2 cut(s) 246, 769
PkrI GCNGC 3 cut(s) 100, 795, 1057
Ple19I CGATCG 1 cut(s) 1012
Psp6I CCWGG 1 cut(s) 144
PspFI CCCAGC 1 cut(s) 115
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 2 cut(s) 489, 1035
PspPI GGNCC 5 cut(s) 488, 710, 892, 986, 1014
PstI CTGCAG 1 cut(s) 798
PsuI RGATCY 2 cut(s) 26, 1033
PsyI GACNNNGTC 1 cut(s) 181
PvuI CGATCG 1 cut(s) 1012
RsaI GTAC 3 cut(s) 509, 800, 1006
RsaNI GTAC 3 cut(s) 508, 799, 1005
RseI CAYNNNNRTG 1 cut(s) 1047
SaqAI TTAA 2 cut(s) 8, 1141
SatI GCNGC 3 cut(s) 99, 794, 1056
Sau3AI GATC 9 cut(s) 3, 26, 387, 534, 636, 851, 880, 1009, 1033
Sau96I GGNCC 5 cut(s) 488, 710, 892, 986, 1014
ScrFI CCNGG 3 cut(s) 146, 244, 849
SduI GDGCHC 2 cut(s) 655, 685
SfaNI GCATC 1 cut(s) 607
SfcI CTRYAG 2 cut(s) 336, 794
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 5 cut(s) 488, 710, 892, 986, 1014
SmiMI CAYNNNNRTG 1 cut(s) 1047
Sse9I AATT 9 cut(s) 279, 290, 304, 364, 565, 600, 674, 689, 1079
SspI AATATT 3 cut(s) 196, 351, 411
SspMI CTAG 2 cut(s) 663, 884
StyD4I CCNGG 3 cut(s) 144, 242, 847
StyI CCWWGG 2 cut(s) 12, 186
TaaI ACNGT 1 cut(s) 541
TaqI TCGA 5 cut(s) 444, 515, 533, 753, 1132
TaqII GACCGA 1 cut(s) 1002
TasI AATT 9 cut(s) 279, 290, 304, 364, 565, 600, 674, 689, 1079
TatI WGTACW 2 cut(s) 507, 798
TfiI GAWTC 3 cut(s) 58, 140, 1117
Tru1I TTAA 2 cut(s) 8, 1141
Tru9I TTAA 2 cut(s) 8, 1141
TseI GCWGC 3 cut(s) 98, 793, 1055
TspDTI ATGAA 3 cut(s) 407, 613, 1130
Tth111I GACNNNGTC 1 cut(s) 181
Van91I CCANNNNNTGG 2 cut(s) 246, 769
VpaK11BI GGWCC 5 cut(s) 488, 710, 892, 986, 1014
XapI RAATTY 2 cut(s) 600, 689
XceI RCATGY 1 cut(s) 1025
XspI CTAG 2 cut(s) 663, 884
Zsp2I ATGCAT 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.