Rorug02G0117000

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000331
Physical Location & Seq
Reverse (-)
9958656 .. 9983503
24848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0117000.1

Sequence Viewer

Length: 5529 bp
ATGGATATTCGCTTCCCTTTCTCGCCGTGCGAGGTCGCCAAGGTCCGCTGCGTCCAGTTCGGCATACTGAGCCCCGACGAAATCAGGCAAATGTCTGTGGTGCAGATTGAGCACAGTGAGACAATGCTGGGGGGAAAGCCGAAGACGGCGGGGTTGAGTGACCCTCGGCTGGGGACAATTGACCGGAAGATGAAGTGTGAGACTTGCACGGCCAACATGGCTGAGTGCCCGGGTCACTTTGGGCACCTGGAGCTGGCCAAGCCCATGTTTCATATTGGGTTCATGAAGACTGTGTTGTCTATAATGCGCTGCGTCTGCTTCAACTGCTCCAAAATCCTAGTTGATGAGGAGGACCACAAGTTTAAGACAGCTTTGAGAATCAAGAACCCAAAAAATAGGCTAAAGAAAATTTTGGACGCGTGCAAAAACAAAACCAAGTGTGAAGGCGGTGATGAGATTGATGTGCAAGAATCGGACGAACCTTTAAAGAAAAGTCGGGGTGGCTGTGGTGCTCAGCAGCCGAAGCTCTCTATAGATGGTATGAAGATGGTTGCAGAGTACAAAGCCCAAAGAAAGAAGAGTGATGACCAGGAACAGCTTCCTGAACCTGTGGAAAGGAAGCAGACCCTTACCGCTGAAAGGGTTCTTAGTGTGTTGAAGAGGATAAGTGATGAAGATTGCCAATTATTGGGATTGAACCCCAAGTATGCACGTCCAGATTGGATGATTTTACAAGTTCTTCCTATTCCTCCACAACCTGTGAGACCCTCTGTGATGATGGACACCTCCTCGAGGAGTGAGGATGATTTAACACATCAGCTGGCCATGATTATCCGCCACAACGAGAATCTGAGGAGACAGGAAAGAAACGGATCACCCGCTCATATCATCTCAGAGTTTGCACAGCTCTTGCAATTCCATATAGCTACATATTTTGATAATGAGTTGCCTGGACTACCAAGGGCCACACAGAGATCAGGGAGGCCTATTAAATCAATTTGTAGTAGGCTTAAGGCGAAGGAAGGTCGGATTAGAGGAAACTTAATGGGGAAGAGGGTTGATTTCTCAGCACGTACGGTTATTACACCAGATCCGAACATTAACATTGATCAATTAGGAGTACCATGGAGTATTGCGTTGAATCTCACATACCCTGAAACTGTGACTCCTTATAATATTGAAAGGTTGAAGGAGCTTGTTGAGTACGGGCCCCATCCTCCACCAGGTAAAACTGGTGCAAAGTATATAATAAGAGATGATGGGCAAAGGCTTGATCTGCGGTACTTGAAGAAAAGTAGTGATCATCATTTGGAGCTTGGTTACAAGGTGGAGCGTCATTTGAATGATGGAGACTTTGTCCTCTTTAATCGTCAACCCAGTCTCCATAAAATGTCTATTATGGGGCACAGAATCAAAATCATGCCTTACTCAACTTTTCGATTAAATCTATCTGTCACTTCGCCTTATAATGCTGATTTTGATGGGGATGAAATGAATATGCATGTTCCCCAGTCATTTGAAACTAGGGCAGAAGTGTTGGAGCTCATGATGGTGCCCAAATGCATTGTGTCCCCTCAATCAAATCGTCCTGTGATGGGAATAGTCCAAGATACCCTGTTGGGTTGTCGTAAGATCACCAAGAGGGATACCTTCATAGAGAAGGATGTTTTCATGAACATCTTGATGTGGTGGGAGGATTTTGATGGAAAAGTACCTGCTCCTGCAATATTGAAACCACGGCCTCTTTGGACTGGGAAACAGGTGTTTAATCTTATAATACCAAAGCAAATAAATCTCCAGAGAACTTCAGCGTGGCACTCTGAGTCAGAAACTGGTAACATTACCCCTGGTGATACTCACGTTAGAATAGAAAAGGGGGAGTTACTTTCTGGAACTCTGTGCAAAAAGGCACTTGGGACATCTACTGGAAGTCTTATACACGTCATATGGGAAGAGGTTGGTCCAGATGCTGCCCGCAAATTCCTTGGTCATACCCAGTGGCTTGTGAATTACTGGCTGTTGCAGAATGCATTTAGTATTGGAATTGGAGATACAATTGCTGATGCAGCAACGATGGAAAAGATCAATGAAACTATTTCCAAGGCAAAAAATGAAGTGAAAGAACTTATCAGTAAAGCCCAATCCAAGAGCCTGGAGCCTGAACCGGGGCGAACTATGATGGATTCCTTTGAAAACAAAGTGAATCAGGTCTTAAATAGGGCTCGTGATGATGCTGGAAGTAGTGCTCAGAAGAGTTTGTCTGAGAGTAACAATCTTAAGGCTATGGTTACAGCTGGCTCTAAGGGAAGTTTTATCAACATATCACAGATGACTGCTTGTGTGGGACAGCAGAATGTTGAGGGTAAACGAATCCCATATGGGTTCATAGACCGTACTTTGCCCCACTTCACTAAGGACGACTATGGACCAGAAAGTCGTGGCTTTGTAGAGAATTCATACCTTCGAGGACTAACTCCACAGGAGTTCTTTTTTCATGCTATGGGAGGTAGGGAAGGTCTTATAGATACTGCAGTTAAGACATCCGAGACTGGGTATATCCAGAGGAGACTGGTGAAGGCTATGGAGGATATTATGGTTAAATATGATGGGACTGTGAGAAATTCGTTGGGGGACGTCATACAGTTTCTCTATGGAGAAGATGGAATGGATGCTGTATGGATAGAATCACAAAAGCTGGATTCTCTTAAAATGAAGAAGAAAGAGTTTGACAAGACTTTCGCCTATGTGTTTGATGATGAAAATTGGAATCCGGACTATATGTTGCCTGAACATGTTGAGGATCTAAAGACTATTAGAGAGTTCCGCAATGTGTTTGATGCAGAAGTTTCAAAACTTGAAACTGATAGGCTTCAACTTGGCACAGAGATTGCTGTCACTGGTGATAATTCTTGGCCATTGCCTGTTAACCTGAAGAGGCTCATTTGGAATGCACAAAAAACTTTTAAAATTGACTTCAGGAGGACTTCTGATATGCATCCAATGGAAATTGTTGAGGCTATTGATAAACTCCAGGAGAGGCTGAGGGTTGTTCCTGGTGATGATTTGTTGAGTGTTGAAGCCCAAAAGAATGCAACTCTCTTTTTCAACATTTTGCTTCGCAGCACTTTCGCAAGTAAAAGGGTGTTGGAGGAGTACAGGCTTACTCGTGAAGCATTTGAGTGGGTTATTGGGGAGATAGAGTCACGTTTCCTGCAATCACTGGTAGCGCCTGGTGAAATGATTGGTTGCGTTGCTGCACAATCTATTGGTGAGCCTGCCACTCAGATGACCCTCAATACCTTCCATTATGCTGGTGTGAGTGCAAAAAATGTTACACTAGGTGTTCCCAGGTTGAGGGAAATTATTAATGTTGCTAAGAGGATCAAAACACCATCTCTGTCTGTCTATTTAAAGCCTGAAGCTAATAAAACCAAGGAGAGGGCCAAGAATGTGCAGTGTGCCTTGGAATACACTACCCTACGAAGTGTTACTCAGGCTACAGAAGTATGGTATGATCCAGATCCAATGAGCACATTAATAGAAGAGGATCTTGATTTTGTGAGATCTTACTACGAAATGCCAGATGAAGAGGTCAACCCCGATAAAATTTCTCCGTGGCTGCTTCGCATTGAGTTGAATCGCGAAATGATGGTGGATAAGAAATTGAGCATGGCTGACATTGCTGAGAAGATTAACCTTGAATTTGATGGTGATTTAACTTGTATTTTTAATGATGACAATGCCGAAAAGTTGATCCTTCGAATTCGTATCATGAATGAGGAAACTCCTAAAGGGGAATCACAAGATGAATCTGCTGAAGATGATGTATTCCTGAAGAAGATTGAAAGTAACATGCTTACAGAAATGGCTCTTCGAGGTATCCCAGATATCAACAAAGTGTTCATAAAGCATGGTAAAGTTAACAAGTTTGATCAGAATGAAGGATTCAAGGCGGAGCAAGAGTGGATGTTGGATACAGAGGGTGTTAATCTTTTAGCTGTCCTGTGCCATGATGATGTTGATGCTAGGAGAACGACAAGCAATCATTTGATTGAAATTATTGAAGTTCTTGGGATTGAGGCAGTTCGCCGGTCTTTGCTGGATGAGTTGCGAGTTGTCATATCGTTTGATGGATCTTATGTGAACTACCGCCATCTGGCTATCCTATGTGATACCATGACGTATCGTGGCCACTTGATGGCCATTACTCGTCATGGTATCAATAGAAATGATACCGGCCCAATGATGCGTTGTTCGTTTGAAGAAACTGTGGATATTCTGCTGGATGCTTCTGTTTTTGCAGAAACGGATCATCTGAGGGGTGTAACAGAAAACATTATGCTAGGCCAGTTGGCACCAATTGGGACTGGAGACTGTGCCCTGTATCTCAATGATGAGATGCTAAAGAATGCTATTGAACTCCAGCTGCCTAGTTATATGGATGGCCTTGATTTTGGCATGACCCCATCGCGTTCTCCAGTGTCAGGAACTCCATATCGTGATGGAATGATGTCACCTAATTATTTGCTGAGCCCAAATATGCGAATGTCTCCCATCTCAGATGCTCAGTTTTCTCCCTATGTTGGTGGAATGGCATTCTCTCCTACTTCATCTCCAGGCTACAGCCCATCATCTCCTGGCTACAGTCCATCCTCCCCTGGATACAGTCCCACGTCCCCTGGTTATAGCCCCACGTCCCCAGGTTACAGCCCCACCTCCCCTGGTTATAGTCCCACTTCTCCAACCTATAGTCCAAGCTCTCCAGGTTATAGTCCAACCAGTCCGGCATATTCTCCTACAAGTCCGTCTTACTCTCCTACATCTCCAAGCTACAGCCCTACCTCTCCAAGCTACAGTCCAACCTCTCCAAGCTACAGCCCGACCTCTCCAAGCTACAGCCCGACTTCCCCCGCTTACAGCCCGACTTCCCCCGCATACAGCCCCACTTCCCCCGCTTACAGCCCCACTTCACCATCATACAGCCCAACATCTCCTTCATACAGCCCCACTTCCCCCTCCTACAGCCCCACTTCGCCGTCCTACAGCCCCACATCCCCCTCCTACAGCCCAACATCCCCCTCCTACAGCCCAACTTCACCTGCCTACAGCCCAACTTCTCCTGGTTACAGCCCTACTTCCCCAAGTTATAGTCCAACCTCTCCCAGTTATAGCCCTACCTCTCCAAGTTACAATCCTCCTTCTGCAAAATATAGCCCATCGGTAGCTTACTCTCCGAGCAGTCCAAGATTGTCACCAGCTAGCCCATATAGTCCTACCTCTCCAAATTACAGCCCCACATCACCGTCATATTCTCCCACATCACCATCTTATTCTCCCTCTAGCCCCTCATACACTCCCGGCAGCCCATATACCTCTGGAGTGAGCCCCGATTACAGCCCAAGTTCTCCACAGTACAGTCCAAGTGCTGGTTACTCACCCAGTCAGCCAGGGTACTCCCCATCATCTACTAGCCAGTACACCCCGCAAACGAGTGAAAAGGATGATAAGGATGACAGGAGCACTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

1842

Amino Acids

205.41

Weight (kDa)

5.88

Isoelectric Point (pI)

64.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 14 - 348 9.7e-115 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 350 - 518 1.1e-71 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 521 - 685 1.3e-52 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4 PF05000 712 - 816 9.7e-38 RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5 PF04998 823 - 1413 3e-104 RNA polymerase Rpb1, domain 5
RNA_pol_Rpb1_6 PF04992 889 - 1073 2e-67 RNA polymerase Rpb1, domain 6
RNA_pol_Rpb1_7 PF04990 1158 - 1292 1.2e-53 RNA polymerase Rpb1, domain 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000331)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03625 AT4G03630 AT4G05460 AT4G05470 AT4G05475 AT4G05490 AT4G05497
fragaria_vesca FvH4_1g14800 FvH4_2g13770 FvH4_2g19530 FvH4_3g09530 FvH4_3g09530 FvH4_3g09552 FvH4_3g09562 FvH4_3g09640 FvH4_6g48070 FvH4_7g32622 FvH4_7g32650 FvH4_7g32650
malus_domestica MD01G1164600.v1.1 MD05G1060300.v1.1 MD05G1276900.v1.1 MD05G1277000.v1.1 MD13G1280000.v1.1
prunus_persica Prupe.4G087700_v2.0.a1 Prupe.4G087800_v2.0.a1 Prupe.4G087800_v2.0.a1 Prupe.4G087900_v2.0.a1
pyrus_communis pycom05g25780 pycom05g25800
rosa_chinensis RchiOBHm_Chr2g0103761 RchiOBHm_Chr2g0167391 RchiOBHm_Chr5g0015131 RchiOBHm_Chr5g0015151 RchiOBHm_Chr5g0015251 RchiOBHm_Chr5g0015321
rosa_laevigata RLG00000010446 RLG00000017256 RLG00000021713 RLG00000021727 RLG00000032174 RLG00000032175 RLG00000032181 RLG00000032186 RLG00000032190 RLG00000032192
rosa_multiflora Rmu_co8228227.1_g000001 Rmu_co8274851.1_g000001 Rmu_co8371751.1_g000001 Rmu_co8379153.1_g000001 Rmu_co8502117.1_g000002 Rmu_sc0000658.1_g000005 Rmu_sc0000658.1_g000012 Rmu_sc0001840.1_g000005 Rmu_sc0001845.1_g000001 Rmu_sc0002209.1_g000007 Rmu_sc0002209.1_g000013 Rmu_sc0002209.1_g000025 Rmu_sc0024563.1_g000001 Rmu_sc0039033.1_g000001
rosa_roxburghii Rroxscaffold_1G00061130 Rroxscaffold_1G00061210 Rroxscaffold_1G00061260 Rroxscaffold_1G00061390 Rroxscaffold_1G00061400 Rroxscaffold_2G00083550 Rroxscaffold_2G00139420
rosa_rugosa Rorug02G0117000 Rorug02G0530100 Rorug05G0024500 Rorug05G0024500 Rorug05G0025100 Rorug05G0025200 Rorug05G0026000 Rorug05G0026700 Rorug06G0384500
rosa_samantha Rh2AG165900 Rh2AG596900 Rh2AG597000 Rh2BG173200 Rh2BG608400 Rh2CG171900 Rh2CG579200 Rh2DG171400 Rh2DG306300 Rh5AG118900 Rh5AG119200 Rh5AG119300 Rh5AG119600 Rh5AG120000 Rh5AG120100 Rh5BG117000 Rh5BG117100 Rh5BG117200 Rh5BG117600 Rh5BG117700 Rh5BG118200 Rh5BG119000 Rh5CG127900 Rh5CG128000 Rh5CG128700 Rh5CG129500 Rh5DG115800 Rh5DG116000 Rh5DG117000 Rh5DG117400 Rh5DG117900 Rh6BG508700 Rh6BG508800
rosa_wichuraiana Rw2G013010 Rw5G010280 Rw5G010370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1173, 1467, 1775
AarI CACCTGC 1 cut(s) 5107
AasI GACNNNNNNGTC 2 cut(s) 295, 2433
AatII GACGTC 1 cut(s) 2637
AbsI CCTCGAGG 1 cut(s) 790
Acc36I ACCTGC 2 cut(s) 1723, 5107
AccB1I GGYRCC 3 cut(s) 243, 1552, 4354
AccB7I CCANNNNNTGG 3 cut(s) 688, 4198, 5426
AccBSI CCGCTC 1 cut(s) 881
AccII CGCG 3 cut(s) 419, 3642, 4471
AccIII TCCGGA 1 cut(s) 2770
AcoI YGGCCR 6 cut(s) 210, 255, 822, 2912, 4189, 4200
AcsI RAATTY 7 cut(s) 408, 1979, 2452, 2620, 3606, 3701, 3762
AcuI CTGAAG 6 cut(s) 1791, 2951, 2959, 3438, 3837, 3854
AcyI GRCGYC 1 cut(s) 2634
AdeI CACNNNGTG 1 cut(s) 3341
AflII CTTAAG 2 cut(s) 1010, 2276
AflIII ACRYGT 3 cut(s) 417, 1939, 2791
AjiI CACGTC 4 cut(s) 713, 1942, 4674, 4695
AjuI GAANNNNNNNTTGG 2 cut(s) 4978, 5010
Alw21I GWGCWC 6 cut(s) 114, 514, 1545, 2248, 3533, 5522
AlwNI CAGNNNCTG 2 cut(s) 1832, 1970
Ama87I CYCGRG 2 cut(s) 229, 790
Aor13HI TCCGGA 1 cut(s) 2770
ApaI GGGCCC 1 cut(s) 1212
ApoI RAATTY 7 cut(s) 408, 1979, 2452, 2620, 3606, 3701, 3762
ArsI GACNNNNNNTTYG 2 cut(s) 2720, 2752
AseI ATTAAT 2 cut(s) 3366, 3536
Asp700I GAANNNNTTC 2 cut(s) 597, 642
AspLEI GCGC 2 cut(s) 309, 3229
AspS9I GGNCC 9 cut(s) 43, 352, 963, 1208, 1209, 1961, 2426, 3441, 4238
AsuC2I CCSGG 4 cut(s) 230, 231, 2166, 5358
AsuII TTCGAA 1 cut(s) 3760
AsuNHI GCTAGC 1 cut(s) 5258
AvaI CYCGRG 2 cut(s) 229, 790
AvaII GGWCC 4 cut(s) 43, 352, 1961, 2426
BaeGI GKGCMC 6 cut(s) 230, 246, 1212, 1407, 1557, 4381
BaeI ACNNNNGTAYC 4 cut(s) 1845, 1878, 2517, 2550
BalI TGGCCA 5 cut(s) 257, 824, 2914, 4191, 4202
BanI GGYRCC 3 cut(s) 243, 1552, 4354
BanII GRGCYC 6 cut(s) 74, 1212, 1545, 2224, 4535, 5387
BauI CACGAG 2 cut(s) 2223, 3165
BbsI GAAGAC 2 cut(s) 149, 293
Bbv12I GWGCWC 6 cut(s) 114, 514, 1545, 2248, 3533, 5522
BbvCI CCTCAGC 1 cut(s) 3041
BceAI ACGGC 5 cut(s) 10, 162, 225, 1754, 5020
BcgI CGANNNNNNTGC 2 cut(s) 3109, 3143
BciVI GTATCC 4 cut(s) 1639, 3890, 3967, 4655
BclI TGATCA 3 cut(s) 1108, 1300, 3931
BcnI CCSGG 4 cut(s) 230, 231, 2166, 5358
BfaI CTAG 9 cut(s) 338, 1524, 3338, 4026, 4343, 4431, 5259, 5340, 5469
BfoI RGCGCY 1 cut(s) 3230
BfrI CTTAAG 2 cut(s) 1010, 2276
BfuAI ACCTGC 2 cut(s) 1723, 5107
BfuI GTATCC 4 cut(s) 1639, 3890, 3967, 4655
BglI GCCNNNNNGGC 1 cut(s) 218
BglII AGATCT 1 cut(s) 3563
BlpI GCTNAGC 2 cut(s) 513, 4529
Bme18I GGWCC 4 cut(s) 43, 352, 1961, 2426
BmeT110I CYCGRG 2 cut(s) 229, 790
BmgBI CACGTC 4 cut(s) 713, 1942, 4674, 4695
BmgT120I GGNCC 9 cut(s) 43, 352, 963, 1208, 1209, 1961, 2426, 3441, 4238
BmiI GGNNCC 6 cut(s) 245, 1210, 1211, 1554, 2157, 4356
BmrI ACTGGG 7 cut(s) 1371, 1504, 1761, 1990, 2559, 5157, 5433
BmtI GCTAGC 1 cut(s) 5262
BmuI ACTGGG 7 cut(s) 1371, 1504, 1761, 1990, 2559, 5157, 5433
BpiI GAAGAC 2 cut(s) 149, 293
BplI GAGNNNNNCTC 2 cut(s) 148, 180
Bpu10I CCTNAGC 1 cut(s) 3041
Bpu1102I GCTNAGC 2 cut(s) 513, 4529
Bpu14I TTCGAA 1 cut(s) 3760
BpuMI CCSGG 4 cut(s) 230, 231, 2166, 5358
BsaAI YACGTR 1 cut(s) 1073
BsaBI GATNNNNATC 2 cut(s) 2994, 3519
BsaHI GRCGYC 1 cut(s) 2634
BsaI GGTCTC 1 cut(s) 757
BsaWI WCCGGW 2 cut(s) 183, 2770
Bse118I RCCGGY 2 cut(s) 4089, 4235
Bse3DI GCAATG 3 cut(s) 2833, 2915, 3678
Bse8I GATNNNNATC 2 cut(s) 2994, 3519
BseAI TCCGGA 1 cut(s) 2770
BseJI GATNNNNATC 2 cut(s) 2994, 3519
BseMI GCAATG 3 cut(s) 2833, 2915, 3678
BseRI GAGGAG 6 cut(s) 362, 778, 808, 868, 2578, 3164
BseSI GKGCMC 6 cut(s) 230, 246, 1212, 1407, 1557, 4381
BseYI CCCAGC 2 cut(s) 127, 169
BsgI GTGCAG 3 cut(s) 122, 3240, 3473
Bsh1236I CGCG 3 cut(s) 419, 3642, 4471
BshNI GGYRCC 3 cut(s) 243, 1552, 4354
BsiHKAI GWGCWC 6 cut(s) 114, 514, 1545, 2248, 3533, 5522
BsiHKCI CYCGRG 2 cut(s) 229, 790
BsiSI CCGG 8 cut(s) 184, 230, 2165, 2771, 4090, 4236, 4784, 5358
BsiWI CGTACG 1 cut(s) 1073
BsmI GAATGC 5 cut(s) 2032, 2953, 3094, 4414, 4595
Bso31I GGTCTC 1 cut(s) 757
BsoBI CYCGRG 2 cut(s) 229, 790
Bsp119I TTCGAA 1 cut(s) 3760
Bsp120I GGGCCC 1 cut(s) 1208
Bsp13I TCCGGA 1 cut(s) 2770
Bsp1720I GCTNAGC 2 cut(s) 513, 4529
Bsp19I CCATGG 1 cut(s) 1124
Bsp68I TCGCGA 1 cut(s) 3642
BspEI TCCGGA 1 cut(s) 2770
BspFNI CGCG 3 cut(s) 419, 3642, 4471
BspHI TCATGA 4 cut(s) 282, 1545, 1671, 3771
BspLI GGNNCC 6 cut(s) 245, 1210, 1211, 1554, 2157, 4356
BspMAI CTGCAG 1 cut(s) 2533
BspMI ACCTGC 2 cut(s) 1723, 5107
BspOI GCTAGC 1 cut(s) 5262
BspQI GCTCTTC 1 cut(s) 3876
BspT104I TTCGAA 1 cut(s) 3760
BspT107I GGYRCC 3 cut(s) 243, 1552, 4354
BspTI CTTAAG 2 cut(s) 1010, 2276
BspTNI GGTCTC 1 cut(s) 757
BsrBI CCGCTC 1 cut(s) 881
BsrDI GCAATG 3 cut(s) 2833, 2915, 3678
BsrFI RCCGGY 2 cut(s) 4089, 4235
BssAI RCCGGY 2 cut(s) 4089, 4235
BssNI GRCGYC 1 cut(s) 2634
BssSI CACGAG 2 cut(s) 2223, 3165
BssT1I CCWWGG 7 cut(s) 39, 959, 1124, 1984, 2100, 3432, 3462
Bst2BI CACGAG 2 cut(s) 2223, 3165
Bst6I CTCTTC 9 cut(s) 572, 653, 1046, 1947, 2246, 2927, 3537, 3582, 3876
BstACI GRCGYC 1 cut(s) 2634
BstAFI CTTAAG 2 cut(s) 1010, 2276
BstBAI YACGTR 1 cut(s) 1073
BstBI TTCGAA 1 cut(s) 3760
BstC8I GCNNGC 7 cut(s) 255, 421, 822, 1975, 2296, 3276, 5260
BstDSI CCRYGG 3 cut(s) 1124, 1736, 3614
BstENI CCTNNNNNAGG 2 cut(s) 790, 1221
BstFNI CGCG 3 cut(s) 419, 3642, 4471
BstH2I RGCGCY 1 cut(s) 3230
BstHHI GCGC 2 cut(s) 309, 3229
BstNSI RCATGY 3 cut(s) 1505, 2795, 3856
BstSLI GKGCMC 6 cut(s) 230, 246, 1212, 1407, 1557, 4381
BstUI CGCG 3 cut(s) 419, 3642, 4471
BstV2I GAAGAC 2 cut(s) 149, 293
BstX2I RGATCY 6 cut(s) 1090, 2800, 3520, 3547, 3563, 4133
BstXI CCANNNNNNTGG 2 cut(s) 2152, 3311
BstYI RGATCY 6 cut(s) 1090, 2800, 3520, 3547, 3563, 4133
BsuI GTATCC 4 cut(s) 1639, 3890, 3967, 4655
BtgI CCRYGG 3 cut(s) 1124, 1736, 3614
BtgZI GCGATG 1 cut(s) 4452
BtrI CACGTC 4 cut(s) 713, 1942, 4674, 4695
BtsI GCAGTG 1 cut(s) 3461
BtsIMutI CAGTG 6 cut(s) 121, 2003, 2895, 3218, 3461, 4485
BtuMI TCGCGA 1 cut(s) 3642
BveI ACCTGC 2 cut(s) 1723, 5107
Cac8I GCNNGC 7 cut(s) 255, 421, 822, 1975, 2296, 3276, 5260
CaiI CAGNNNCTG 2 cut(s) 1832, 1970
CciI TCATGA 4 cut(s) 282, 1545, 1671, 3771
CfoI GCGC 2 cut(s) 309, 3229
Cfr10I RCCGGY 2 cut(s) 4089, 4235
Cfr13I GGNCC 9 cut(s) 43, 352, 963, 1208, 1209, 1961, 2426, 3441, 4238
Cfr9I CCCGGG 1 cut(s) 229
CseI GACGC 4 cut(s) 40, 301, 425, 1322
CsiI ACCWGGT 1 cut(s) 1222
DraI TTTAAA 3 cut(s) 486, 2965, 3411
DraIII CACNNNGTG 1 cut(s) 3341
DrdI GACNNNNNNGTC 2 cut(s) 295, 2433
DseDI GACNNNNNNGTC 2 cut(s) 295, 2433
EaeI YGGCCR 6 cut(s) 210, 255, 822, 2912, 4189, 4200
Eam1104I CTCTTC 9 cut(s) 572, 653, 1046, 1947, 2246, 2927, 3537, 3582, 3876
EarI CTCTTC 9 cut(s) 572, 653, 1046, 1947, 2246, 2927, 3537, 3582, 3876
EciI GGCGGA 2 cut(s) 824, 3968
Ecl136II GAGCTC 1 cut(s) 1543
Eco130I CCWWGG 7 cut(s) 39, 959, 1124, 1984, 2100, 3432, 3462
Eco147I AGGCCT 1 cut(s) 985
Eco24I GRGCYC 6 cut(s) 74, 1212, 1545, 2224, 4535, 5387
Eco31I GGTCTC 1 cut(s) 757
Eco32I GATATC 1 cut(s) 3889
Eco47I GGWCC 4 cut(s) 43, 352, 1961, 2426
Eco53kI GAGCTC 1 cut(s) 1543
Eco57I CTGAAG 6 cut(s) 1791, 2951, 2959, 3438, 3837, 3854
Eco88I CYCGRG 2 cut(s) 229, 790
EcoICRI GAGCTC 1 cut(s) 1543
EcoNI CCTNNNNNAGG 2 cut(s) 790, 1221
EcoO109I RGGNCCY 1 cut(s) 1209
EcoRI GAATTC 2 cut(s) 2452, 3762
EcoRV GATATC 1 cut(s) 3889
EcoT14I CCWWGG 7 cut(s) 39, 959, 1124, 1984, 2100, 3432, 3462
EcoT22I ATGCAT 4 cut(s) 1503, 1565, 2032, 2997
EcoT38I GRGCYC 6 cut(s) 74, 1212, 1545, 2224, 4535, 5387
ErhI CCWWGG 7 cut(s) 39, 959, 1124, 1984, 2100, 3432, 3462
FalI AAGNNNNNCTT 4 cut(s) 3534, 3566, 4792, 4824
FauI CCCGC 7 cut(s) 142, 886, 1982, 4918, 4939, 4960, 5490
FauNDI CATATG 2 cut(s) 1946, 2377
FbaI TGATCA 3 cut(s) 1108, 1300, 3931
FriOI GRGCYC 6 cut(s) 74, 1212, 1545, 2224, 4535, 5387
FspBI CTAG 9 cut(s) 338, 1524, 3338, 4026, 4343, 4431, 5259, 5340, 5469
GlaI GCGC 2 cut(s) 308, 3228
GsaI CCCAGC 2 cut(s) 131, 173
HaeII RGCGCY 1 cut(s) 3230
HapII CCGG 8 cut(s) 184, 230, 2165, 2771, 4090, 4236, 4784, 5358
HgaI GACGC 4 cut(s) 40, 301, 425, 1322
HhaI GCGC 2 cut(s) 309, 3229
Hin1I GRCGYC 1 cut(s) 2634
Hin6I GCGC 2 cut(s) 307, 3227
HinP1I GCGC 2 cut(s) 307, 3227
HincII GTYRAC 4 cut(s) 1373, 2926, 3595, 3922
HindII GTYRAC 4 cut(s) 1373, 2926, 3595, 3922
HpaI GTTAAC 2 cut(s) 2926, 3922
HpaII CCGG 8 cut(s) 184, 230, 2165, 2771, 4090, 4236, 4784, 5358
Hpy166II GTNNAC 7 cut(s) 1373, 2366, 2926, 3595, 3922, 4144, 5478
Hpy8I GTNNAC 7 cut(s) 1373, 2366, 2926, 3595, 3922, 4144, 5478
Hpy99I CGWCG 1 cut(s) 80
HpyCH4IV ACGT 9 cut(s) 712, 1072, 1860, 1941, 2634, 3205, 4181, 4673, 4694
HpySE526I ACGT 9 cut(s) 712, 1072, 1860, 1941, 2634, 3205, 4181, 4673, 4694
Hsp92I GRCGYC 1 cut(s) 2634
HspAI GCGC 2 cut(s) 307, 3227
Kpn2I TCCGGA 1 cut(s) 2770
Ksp22I TGATCA 3 cut(s) 1108, 1300, 3931
KspAI GTTAAC 2 cut(s) 2926, 3922
LguI GCTCTTC 1 cut(s) 3876
MabI ACCWGGT 1 cut(s) 1222
MaeI CTAG 9 cut(s) 338, 1524, 3338, 4026, 4343, 4431, 5259, 5340, 5469
MaeII ACGT 9 cut(s) 712, 1072, 1860, 1941, 2634, 3205, 4181, 4673, 4694
MbiI CCGCTC 1 cut(s) 881
MfeI CAATTG 3 cut(s) 177, 2055, 4359
MflI RGATCY 6 cut(s) 1090, 2800, 3520, 3547, 3563, 4133
MlsI TGGCCA 5 cut(s) 257, 824, 2914, 4191, 4202
MluI ACGCGT 1 cut(s) 417
MluNI TGGCCA 5 cut(s) 257, 824, 2914, 4191, 4202
MlyI GAGTC 3 cut(s) 1159, 1832, 3209
MmeI TCCRAC 8 cut(s) 1007, 1518, 3126, 3951, 4766, 4799, 4883, 5177
Mox20I TGGCCA 5 cut(s) 257, 824, 2914, 4191, 4202
Mph1103I ATGCAT 4 cut(s) 1503, 1565, 2032, 2997
MroI TCCGGA 1 cut(s) 2770
MroXI GAANNNNTTC 2 cut(s) 597, 642
MscI TGGCCA 5 cut(s) 257, 824, 2914, 4191, 4202
MslI CAYNNNNRTG 6 cut(s) 1341, 1550, 1682, 3178, 3284, 4014
Msp20I TGGCCA 5 cut(s) 257, 824, 2914, 4191, 4202
MspA1I CMGCKG 5 cut(s) 48, 635, 820, 2294, 4426
MspCI CTTAAG 2 cut(s) 1010, 2276
MspI CCGG 8 cut(s) 184, 230, 2165, 2771, 4090, 4236, 4784, 5358
MunI CAATTG 3 cut(s) 177, 2055, 4359
Mva1269I GAATGC 5 cut(s) 2032, 2953, 3094, 4414, 4595
MvnI CGCG 3 cut(s) 419, 3642, 4471
NciI CCSGG 4 cut(s) 230, 231, 2166, 5358
NcoI CCATGG 1 cut(s) 1124
NdeI CATATG 2 cut(s) 1946, 2377
NheI GCTAGC 1 cut(s) 5258
NlaIV GGNNCC 6 cut(s) 245, 1210, 1211, 1554, 2157, 4356
NmeAIII GCCGAG 1 cut(s) 145
NmuCI GTSAC 8 cut(s) 158, 233, 1162, 1453, 2893, 3201, 4512, 5250
NruI TCGCGA 1 cut(s) 3642
NsiI ATGCAT 4 cut(s) 1503, 1565, 2032, 2997
NspI RCATGY 3 cut(s) 1505, 2795, 3856
NspV TTCGAA 1 cut(s) 3760
PaeR7I CTCGAG 1 cut(s) 790
PagI TCATGA 4 cut(s) 282, 1545, 1671, 3771
PaqCI CACCTGC 1 cut(s) 5107
PceI AGGCCT 1 cut(s) 985
PciI ACATGT 1 cut(s) 2791
PciSI GCTCTTC 1 cut(s) 3876
PctI GAATGC 5 cut(s) 2032, 2953, 3094, 4414, 4595
PdmI GAANNNNTTC 2 cut(s) 597, 642
Pfl23II CGTACG 1 cut(s) 1073
PflFI GACNNNGTC 1 cut(s) 1355
PflMI CCANNNNNTGG 3 cut(s) 688, 4198, 5426
PfoI TCCNGGA 1 cut(s) 3030
PleI GAGTC 3 cut(s) 1159, 1831, 3208
PpsI GAGTC 3 cut(s) 1159, 1831, 3208
Ppu21I YACGTR 1 cut(s) 1073
PscI ACATGT 1 cut(s) 2791
PshBI ATTAAT 2 cut(s) 3366, 3536
PsiI TTATAA 3 cut(s) 1173, 1467, 1775
Psp124BI GAGCTC 1 cut(s) 1545
PspFI CCCAGC 2 cut(s) 127, 169
PspLI CGTACG 1 cut(s) 1073
PspN4I GGNNCC 6 cut(s) 245, 1210, 1211, 1554, 2157, 4356
PspOMI GGGCCC 1 cut(s) 1208
PspPI GGNCC 9 cut(s) 43, 352, 963, 1208, 1209, 1961, 2426, 3441, 4238
PspXI VCTCGAGB 1 cut(s) 790
PstI CTGCAG 1 cut(s) 2533
PstNI CAGNNNCTG 2 cut(s) 1832, 1970
PsuI RGATCY 6 cut(s) 1090, 2800, 3520, 3547, 3563, 4133
PsyI GACNNNGTC 1 cut(s) 1355
PvuII CAGCTG 3 cut(s) 820, 2294, 4426
RruI TCGCGA 1 cut(s) 3642
RseI CAYNNNNRTG 6 cut(s) 1341, 1550, 1682, 3178, 3284, 4014
SacI GAGCTC 1 cut(s) 1545
SapI GCTCTTC 1 cut(s) 3876
Sau96I GGNCC 9 cut(s) 43, 352, 963, 1208, 1209, 1961, 2426, 3441, 4238
SchI GAGTC 3 cut(s) 1159, 1832, 3209
SexAI ACCWGGT 1 cut(s) 1222
Sfr274I CTCGAG 1 cut(s) 790
SfuI TTCGAA 1 cut(s) 3760
SinI GGWCC 4 cut(s) 43, 352, 1961, 2426
SlaI CTCGAG 1 cut(s) 790
SmaI CCCGGG 1 cut(s) 231
SmiMI CAYNNNNRTG 6 cut(s) 1341, 1550, 1682, 3178, 3284, 4014
SmlI CTYRAG 3 cut(s) 790, 1010, 2276
SmoI CTYRAG 3 cut(s) 790, 1010, 2276
SseBI AGGCCT 1 cut(s) 985
SspI AATATT 2 cut(s) 1177, 1728
SspMI CTAG 9 cut(s) 338, 1524, 3338, 4026, 4343, 4431, 5259, 5340, 5469
SstI GAGCTC 1 cut(s) 1545
StuI AGGCCT 1 cut(s) 985
StyI CCWWGG 7 cut(s) 39, 959, 1124, 1984, 2100, 3432, 3462
TaiI ACGT 9 cut(s) 715, 1075, 1863, 1944, 2637, 3208, 4184, 4676, 4697
TaqI TCGA 5 cut(s) 791, 1438, 2464, 3760, 3874
TatI WGTACW 4 cut(s) 558, 3153, 5412, 5475
TscAI CASTG 6 cut(s) 121, 2003, 2902, 3225, 3461, 4485
TseFI GTSAC 8 cut(s) 158, 233, 1162, 1453, 2893, 3201, 4512, 5250
Tsp45I GTSAC 8 cut(s) 158, 233, 1162, 1453, 2893, 3201, 4512, 5250
TspGWI ACGGA 4 cut(s) 885, 3603, 4322, 4794
TspMI CCCGGG 1 cut(s) 229
TspRI CASTG 6 cut(s) 121, 2003, 2902, 3225, 3461, 4485
Tth111I GACNNNGTC 1 cut(s) 1355
Van91I CCANNNNNTGG 3 cut(s) 688, 4198, 5426
Vha464I CTTAAG 2 cut(s) 1010, 2276
VpaK11BI GGWCC 4 cut(s) 43, 352, 1961, 2426
VspI ATTAAT 2 cut(s) 3366, 3536
XagI CCTNNNNNAGG 2 cut(s) 790, 1221
XapI RAATTY 7 cut(s) 408, 1979, 2452, 2620, 3606, 3701, 3762
XceI RCATGY 3 cut(s) 1505, 2795, 3856
XcmI CCANNNNNNNNNTGG 1 cut(s) 1743
XhoI CTCGAG 1 cut(s) 790
XmaI CCCGGG 1 cut(s) 229
XmnI GAANNNNTTC 2 cut(s) 597, 642
XspI CTAG 9 cut(s) 338, 1524, 3338, 4026, 4343, 4431, 5259, 5340, 5469
ZraI GACGTC 1 cut(s) 2635
Zsp2I ATGCAT 4 cut(s) 1503, 1565, 2032, 2997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.