pycom05g25780

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000331
Physical Location & Seq
Reverse (-)
27110814 .. 27112818
2005 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25780.2

Sequence Viewer

Length: 975 bp
ATGACCTCCTCCGCTCGACCCCCTCCAGACCGGGGCCGAAACTGGACCGAACTGCCGGAGGACGTCACGGTTTCAATACTCTCTCGGCTCGGAGCCATCGAAATCCTGACGAGCGCTCAGATGGTGTGCGCGACGTGGAACAACATTTGCAAGGACCCGCTGATGTGGTGCACCATCGACCTGCGCGATCTTGATCACCAGAAACACATTCATTACGACTTGGAGAAGATGTGCCGCCACGCCGTTGACCGCAGCTCTGGAAATCTGGTCTGTATCAATGTCGAAGATTTCGGCTCCGATGAGCTTCTCACGTACATCACCAACTGTTCAAGTGGAATCAAACGTCTCCGACTTGTCCGTTGCGATGGCATAACGAATGAGGGACTGGCTAAAGTGGCTTCTAAACTTCCACTCTTGGAAGACCTTGAAATTTCGTATTGTGCATTCTCACATGAACCTCTAGAAGTTGTAGGGTCTTCTTGCCCTCTTTTGAAATCATTCAAATTTAACAACCGGTGGTACAGATGGTCAGATGAAGAGTGTGACGACGATGCACTTGCTATAGCAGGAACGATGCATGATTTACGCCACCTCCAGCTTTTTGGGAATAAGCTAACGAACGATGGCTTGCTGGCCATTCTTGACCATTGTCCTCAGCTCGAGTTGCTTGATCTTCGGCAGTGCTTCAATCTTAAGCTGGGAGGAAAGTTGAGAAGAAGATGTGCTGAACAAGTGAAAAAATTGTGGTTTCCTGATGATTCCACTCTTGACTATGAATTTGATTCTGCAATTGATTACCATGAAGACAACCCATTTGGGTATTCGGATATAGATTTGATGTCTGATGATGATAAGGATTATGACTTCGGTAACCGAAAAGTAGAAGATGATGAGGAGTATGACTTCAACCATGGGAGTGGTTCTGATGACTGCCGTGAGGTTGAGGATAATGATGTTTATCCATGGATTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

325

Amino Acids

37.16

Weight (kDa)

4.59

Isoelectric Point (pI)

52.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000331)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03625 AT4G03630 AT4G05460 AT4G05470 AT4G05475 AT4G05490 AT4G05497
fragaria_vesca FvH4_1g14800 FvH4_2g13770 FvH4_2g19530 FvH4_3g09530 FvH4_3g09530 FvH4_3g09552 FvH4_3g09562 FvH4_3g09640 FvH4_6g48070 FvH4_7g32622 FvH4_7g32650 FvH4_7g32650
malus_domestica MD01G1164600.v1.1 MD05G1060300.v1.1 MD05G1276900.v1.1 MD05G1277000.v1.1 MD13G1280000.v1.1
prunus_persica Prupe.4G087700_v2.0.a1 Prupe.4G087800_v2.0.a1 Prupe.4G087800_v2.0.a1 Prupe.4G087900_v2.0.a1
pyrus_communis pycom05g25780 pycom05g25800
rosa_chinensis RchiOBHm_Chr2g0103761 RchiOBHm_Chr2g0167391 RchiOBHm_Chr5g0015131 RchiOBHm_Chr5g0015151 RchiOBHm_Chr5g0015251 RchiOBHm_Chr5g0015321
rosa_laevigata RLG00000010446 RLG00000017256 RLG00000021713 RLG00000021727 RLG00000032174 RLG00000032175 RLG00000032181 RLG00000032186 RLG00000032190 RLG00000032192
rosa_multiflora Rmu_co8228227.1_g000001 Rmu_co8274851.1_g000001 Rmu_co8371751.1_g000001 Rmu_co8379153.1_g000001 Rmu_co8502117.1_g000002 Rmu_sc0000658.1_g000005 Rmu_sc0000658.1_g000012 Rmu_sc0001840.1_g000005 Rmu_sc0001845.1_g000001 Rmu_sc0002209.1_g000007 Rmu_sc0002209.1_g000013 Rmu_sc0002209.1_g000025 Rmu_sc0024563.1_g000001 Rmu_sc0039033.1_g000001
rosa_roxburghii Rroxscaffold_1G00061130 Rroxscaffold_1G00061210 Rroxscaffold_1G00061260 Rroxscaffold_1G00061390 Rroxscaffold_1G00061400 Rroxscaffold_2G00083550 Rroxscaffold_2G00139420
rosa_rugosa Rorug02G0117000 Rorug02G0530100 Rorug05G0024500 Rorug05G0024500 Rorug05G0025100 Rorug05G0025200 Rorug05G0026000 Rorug05G0026700 Rorug06G0384500
rosa_samantha Rh2AG165900 Rh2AG596900 Rh2AG597000 Rh2BG173200 Rh2BG608400 Rh2CG171900 Rh2CG579200 Rh2DG171400 Rh2DG306300 Rh5AG118900 Rh5AG119200 Rh5AG119300 Rh5AG119600 Rh5AG120000 Rh5AG120100 Rh5BG117000 Rh5BG117100 Rh5BG117200 Rh5BG117600 Rh5BG117700 Rh5BG118200 Rh5BG119000 Rh5CG127900 Rh5CG128000 Rh5CG128700 Rh5CG129500 Rh5DG115800 Rh5DG116000 Rh5DG117000 Rh5DG117400 Rh5DG117900 Rh6BG508700 Rh6BG508800
rosa_wichuraiana Rw2G013010 Rw5G010280 Rw5G010370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 66
Acc36I ACCTGC 1 cut(s) 189
AccBSI CCGCTC 1 cut(s) 14
AccII CGCG 2 cut(s) 131, 186
AciI CCGC 4 cut(s) 12, 158, 235, 250
AcoI YGGCCR 1 cut(s) 633
AcsI RAATTY 3 cut(s) 429, 503, 776
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 2 cut(s) 314, 521
AfeI AGCGCT 1 cut(s) 115
AfiI CCNNNNNNNGG 1 cut(s) 32
AflII CTTAAG 1 cut(s) 692
AgeI ACCGGT 1 cut(s) 513
AgsI TTSAA 7 cut(s) 75, 330, 428, 493, 502, 688, 907
AjiI CACGTC 1 cut(s) 135
AluBI AGCT 6 cut(s) 255, 304, 598, 613, 658, 697
AluI AGCT 6 cut(s) 255, 304, 598, 613, 658, 697
Alw21I GWGCWC 1 cut(s) 173
Alw26I GTCTC 1 cut(s) 350
Alw44I GTGCAC 1 cut(s) 169
Ama87I CYCGRG 1 cut(s) 659
Aor51HI AGCGCT 1 cut(s) 115
AoxI GGCC 2 cut(s) 34, 633
ApaLI GTGCAC 1 cut(s) 169
ApeKI GCWGC 1 cut(s) 252
ApoI RAATTY 3 cut(s) 429, 503, 776
ArsI GACNNNNNNTTYG 4 cut(s) 761, 793, 797, 829
AsiGI ACCGGT 1 cut(s) 513
Asp700I GAANNNNTTC 1 cut(s) 497
AspLEI GCGC 3 cut(s) 116, 131, 186
AspS9I GGNCC 3 cut(s) 34, 45, 154
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 2 cut(s) 188, 310
AvaI CYCGRG 1 cut(s) 659
AvaII GGWCC 2 cut(s) 45, 154
BaeGI GKGCMC 1 cut(s) 173
BalI TGGCCA 1 cut(s) 635
BbsI GAAGAC 3 cut(s) 426, 468, 810
Bbv12I GWGCWC 1 cut(s) 173
BbvCI CCTCAGC 1 cut(s) 654
BbvI GCAGC 1 cut(s) 264
BccI CCATC 6 cut(s) 104, 115, 182, 359, 519, 617
BceAI ACGGC 2 cut(s) 227, 918
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BclI TGATCA 1 cut(s) 193
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 350
BfaI CTAG 1 cut(s) 461
BfmI CTRYAG 1 cut(s) 561
BfoI RGCGCY 1 cut(s) 117
BfrI CTTAAG 1 cut(s) 692
BfuAI ACCTGC 1 cut(s) 189
BisI GCNGC 2 cut(s) 235, 253
BlsI GCNGC 2 cut(s) 236, 254
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 2 cut(s) 45, 154
BmeT110I CYCGRG 1 cut(s) 659
BmgBI CACGTC 1 cut(s) 135
BmgT120I GGNCC 3 cut(s) 34, 45, 154
BmiI GGNNCC 4 cut(s) 35, 94, 156, 295
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 2 cut(s) 541, 564
BoxI GACNNNNGTC 1 cut(s) 648
BpiI GAAGAC 3 cut(s) 426, 468, 810
BpmI CTGGAG 2 cut(s) 9, 578
Bpu10I CCTNAGC 1 cut(s) 654
BpuMI CCSGG 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 312
BsaBI GATNNNNATC 2 cut(s) 192, 957
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 3 cut(s) 31, 910, 962
BsaWI WCCGGW 1 cut(s) 513
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 576, 606
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse118I RCCGGY 1 cut(s) 513
Bse1I ACTGG 2 cut(s) 47, 390
Bse8I GATNNNNATC 2 cut(s) 192, 957
BseDI CCNNGG 3 cut(s) 31, 910, 962
BseJI GATNNNNATC 2 cut(s) 192, 957
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 2 cut(s) 131, 668
BseNI ACTGG 2 cut(s) 47, 390
BseRI GAGGAG 1 cut(s) 908
BseSI GKGCMC 1 cut(s) 173
BseXI GCAGC 1 cut(s) 264
BseYI CCCAGC 1 cut(s) 697
Bsh1236I CGCG 2 cut(s) 131, 186
BshFI GGCC 2 cut(s) 36, 635
BshTI ACCGGT 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 173
BsiHKCI CYCGRG 1 cut(s) 659
BsiSI CCGG 3 cut(s) 31, 56, 514
BslFI GGGAC 1 cut(s) 396
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 1 cut(s) 350
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 396
BsmI GAATGC 1 cut(s) 443
BsnI GGCC 2 cut(s) 36, 635
BsoBI CYCGRG 1 cut(s) 659
Bsp1286I GDGCHC 1 cut(s) 173
Bsp143I GATC 3 cut(s) 187, 193, 670
Bsp19I CCATGG 2 cut(s) 910, 962
BspACI CCGC 4 cut(s) 12, 158, 235, 250
BspANI GGCC 2 cut(s) 36, 635
BspCNI CTCAG 2 cut(s) 130, 667
BspFNI CGCG 2 cut(s) 131, 186
BspLI GGNNCC 4 cut(s) 35, 94, 156, 295
BspMI ACCTGC 1 cut(s) 189
BspTI CTTAAG 1 cut(s) 692
BsrBI CCGCTC 1 cut(s) 14
BsrFI RCCGGY 1 cut(s) 513
BsrI ACTGG 2 cut(s) 47, 390
BssAI RCCGGY 1 cut(s) 513
BssECI CCNNGG 3 cut(s) 31, 910, 962
BssMI GATC 3 cut(s) 187, 193, 670
BssNI GRCGYC 1 cut(s) 63
BssT1I CCWWGG 2 cut(s) 910, 962
Bst4CI ACNGT 2 cut(s) 70, 326
Bst6I CTCTTC 1 cut(s) 531
BstACI GRCGYC 1 cut(s) 63
BstAFI CTTAAG 1 cut(s) 692
BstBAI YACGTR 1 cut(s) 312
BstC8I GCNNGC 2 cut(s) 629, 633
BstDEI CTNAG 2 cut(s) 117, 654
BstDSI CCRYGG 2 cut(s) 910, 962
BstEII GGTNACC 1 cut(s) 869
BstFNI CGCG 2 cut(s) 131, 186
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 3 cut(s) 116, 131, 186
BstKTI GATC 3 cut(s) 190, 196, 673
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 3 cut(s) 187, 193, 670
BstMWI GCNNNNNNNGC 2 cut(s) 395, 664
BstPAI GACNNNNGTC 1 cut(s) 648
BstPI GGTNACC 1 cut(s) 869
BstSCI CCNGG 1 cut(s) 30
BstSFI CTRYAG 1 cut(s) 561
BstSLI GKGCMC 1 cut(s) 173
BstUI CGCG 2 cut(s) 131, 186
BstV1I GCAGC 1 cut(s) 264
BstV2I GAAGAC 3 cut(s) 426, 468, 810
BstXI CCANNNNNNTGG 2 cut(s) 602, 917
BsuRI GGCC 2 cut(s) 36, 635
BtgI CCRYGG 2 cut(s) 910, 962
BtgZI GCGATG 1 cut(s) 378
BtrI CACGTC 1 cut(s) 135
BtsI GCAGTG 1 cut(s) 686
BtsIMutI CAGTG 1 cut(s) 686
BveI ACCTGC 1 cut(s) 189
Cac8I GCNNGC 2 cut(s) 629, 633
CfoI GCGC 3 cut(s) 116, 131, 186
Cfr10I RCCGGY 1 cut(s) 513
Cfr13I GGNCC 3 cut(s) 34, 45, 154
Csp6I GTAC 2 cut(s) 313, 520
CspAI ACCGGT 1 cut(s) 513
CviAII CATG 5 cut(s) 452, 578, 800, 911, 963
CviQI GTAC 2 cut(s) 313, 520
DdeI CTNAG 2 cut(s) 117, 654
DpnI GATC 3 cut(s) 189, 195, 672
DpnII GATC 3 cut(s) 187, 193, 670
EaeI YGGCCR 1 cut(s) 633
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
Eco130I CCWWGG 2 cut(s) 910, 962
Eco47I GGWCC 2 cut(s) 45, 154
Eco47III AGCGCT 1 cut(s) 115
Eco88I CYCGRG 1 cut(s) 659
Eco91I GGTNACC 1 cut(s) 869
EcoO109I RGGNCCY 1 cut(s) 154
EcoO65I GGTNACC 1 cut(s) 869
EcoT14I CCWWGG 2 cut(s) 910, 962
EcoT22I ATGCAT 1 cut(s) 579
ErhI CCWWGG 2 cut(s) 910, 962
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 5 cut(s) 455, 581, 803, 914, 966
FaqI GGGAC 1 cut(s) 396
FatI CATG 5 cut(s) 451, 577, 799, 910, 962
FauI CCCGC 1 cut(s) 165
FbaI TGATCA 1 cut(s) 193
Fnu4HI GCNGC 2 cut(s) 235, 253
Fsp4HI GCNGC 2 cut(s) 235, 253
FspBI CTAG 1 cut(s) 461
GlaI GCGC 3 cut(s) 115, 130, 185
GluI GCNGC 2 cut(s) 235, 253
GsaI CCCAGC 1 cut(s) 701
GsuI CTGGAG 2 cut(s) 9, 578
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 2 cut(s) 36, 635
HapII CCGG 3 cut(s) 31, 56, 514
HhaI GCGC 3 cut(s) 116, 131, 186
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 5 cut(s) 455, 581, 803, 914, 966
Hin6I GCGC 3 cut(s) 114, 129, 184
HinP1I GCGC 3 cut(s) 114, 129, 184
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 3 cut(s) 336, 758, 782
HpaII CCGG 3 cut(s) 31, 56, 514
HphI GGTGA 2 cut(s) 188, 310
Hpy166II GTNNAC 2 cut(s) 171, 247
Hpy188I TCNGA 9 cut(s) 92, 120, 298, 350, 532, 826, 844, 925, 974
Hpy188III TCNNGA 8 cut(s) 26, 106, 191, 258, 461, 641, 752, 767
Hpy8I GTNNAC 2 cut(s) 171, 247
Hpy99I CGWCG 2 cut(s) 136, 551
HpyCH4III ACNGT 2 cut(s) 70, 326
HpyCH4IV ACGT 4 cut(s) 63, 134, 311, 343
HpyCH4V TGCA 6 cut(s) 150, 171, 443, 554, 577, 788
HpyF10VI GCNNNNNNNGC 2 cut(s) 395, 664
HpyF3I CTNAG 2 cut(s) 117, 654
HpySE526I ACGT 4 cut(s) 63, 134, 311, 343
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 5 cut(s) 455, 581, 803, 914, 966
HspAI GCGC 3 cut(s) 114, 129, 184
Ksp22I TGATCA 1 cut(s) 193
Kzo9I GATC 3 cut(s) 187, 193, 670
LmnI GCTCC 2 cut(s) 92, 299
Lsp1109I GCAGC 1 cut(s) 264
LweI GCATC 2 cut(s) 541, 564
MaeI CTAG 1 cut(s) 461
MaeII ACGT 4 cut(s) 63, 134, 311, 343
MaeIII GTNAC 3 cut(s) 64, 542, 869
MalI GATC 3 cut(s) 189, 195, 672
MbiI CCGCTC 1 cut(s) 14
MboI GATC 3 cut(s) 187, 193, 670
MfeI CAATTG 1 cut(s) 789
MhlI GDGCHC 1 cut(s) 173
MlsI TGGCCA 1 cut(s) 635
MluCI AATT 5 cut(s) 429, 503, 740, 776, 789
MluNI TGGCCA 1 cut(s) 635
MmeI TCCRAC 1 cut(s) 373
Mox20I TGGCCA 1 cut(s) 635
Mph1103I ATGCAT 1 cut(s) 579
MroXI GAANNNNTTC 1 cut(s) 497
MscI TGGCCA 1 cut(s) 635
MseI TTAA 2 cut(s) 507, 693
MslI CAYNNNNRTG 1 cut(s) 915
Msp20I TGGCCA 1 cut(s) 635
MspA1I CMGCKG 1 cut(s) 160
MspCI CTTAAG 1 cut(s) 692
MspI CCGG 3 cut(s) 31, 56, 514
MspR9I CCNGG 1 cut(s) 32
MunI CAATTG 1 cut(s) 789
Mva1269I GAATGC 1 cut(s) 443
MvnI CGCG 2 cut(s) 131, 186
MwoI GCNNNNNNNGC 2 cut(s) 395, 664
NciI CCSGG 1 cut(s) 32
NcoI CCATGG 2 cut(s) 910, 962
NdeII GATC 3 cut(s) 187, 193, 670
NlaIII CATG 5 cut(s) 455, 581, 803, 914, 966
NlaIV GGNNCC 4 cut(s) 35, 94, 156, 295
NmeAIII GCCGAG 1 cut(s) 64
NmuCI GTSAC 2 cut(s) 64, 542
NsiI ATGCAT 1 cut(s) 579
PaeR7I CTCGAG 1 cut(s) 659
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 183
PctI GAATGC 1 cut(s) 443
PdmI GAANNNNTTC 1 cut(s) 497
PfeI GAWTC 3 cut(s) 336, 758, 782
PinAI ACCGGT 1 cut(s) 513
PkrI GCNGC 2 cut(s) 236, 254
Ppu21I YACGTR 1 cut(s) 312
PpuMI RGGWCCY 1 cut(s) 154
PshAI GACNNNNGTC 1 cut(s) 648
Psp5II RGGWCCY 1 cut(s) 154
PspEI GGTNACC 1 cut(s) 869
PspFI CCCAGC 1 cut(s) 697
PspN4I GGNNCC 4 cut(s) 35, 94, 156, 295
PspPI GGNCC 3 cut(s) 34, 45, 154
PspPPI RGGWCCY 1 cut(s) 154
PspXI VCTCGAGB 1 cut(s) 659
RsaI GTAC 2 cut(s) 314, 521
RsaNI GTAC 2 cut(s) 313, 520
RseI CAYNNNNRTG 1 cut(s) 915
SaqAI TTAA 2 cut(s) 507, 693
SatI GCNGC 2 cut(s) 235, 253
Sau3AI GATC 3 cut(s) 187, 193, 670
Sau96I GGNCC 3 cut(s) 34, 45, 154
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 1 cut(s) 173
SfaNI GCATC 2 cut(s) 541, 564
SfcI CTRYAG 1 cut(s) 561
Sfr274I CTCGAG 1 cut(s) 659
SinI GGWCC 2 cut(s) 45, 154
SlaI CTCGAG 1 cut(s) 659
SmiMI CAYNNNNRTG 1 cut(s) 915
SmlI CTYRAG 2 cut(s) 659, 692
SmoI CTYRAG 2 cut(s) 659, 692
Sse9I AATT 5 cut(s) 429, 503, 740, 776, 789
SsiI CCGC 4 cut(s) 12, 158, 235, 250
SspMI CTAG 1 cut(s) 461
StyD4I CCNGG 1 cut(s) 30
StyI CCWWGG 2 cut(s) 910, 962
TaaI ACNGT 2 cut(s) 70, 326
TaiI ACGT 4 cut(s) 66, 137, 314, 346
TaqI TCGA 5 cut(s) 16, 99, 177, 282, 660
TaqII GACCGA 1 cut(s) 62
TasI AATT 5 cut(s) 429, 503, 740, 776, 789
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 3 cut(s) 336, 758, 782
Tru1I TTAA 2 cut(s) 507, 693
Tru9I TTAA 2 cut(s) 507, 693
TscAI CASTG 1 cut(s) 686
TseFI GTSAC 2 cut(s) 64, 542
TseI GCWGC 1 cut(s) 252
Tsp45I GTSAC 2 cut(s) 64, 542
TspDTI ATGAA 5 cut(s) 200, 468, 549, 789, 816
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 1 cut(s) 686
Vha464I CTTAAG 1 cut(s) 692
VneI GTGCAC 1 cut(s) 169
VpaK11BI GGWCC 2 cut(s) 45, 154
XapI RAATTY 3 cut(s) 429, 503, 776
XbaI TCTAGA 1 cut(s) 460
XhoI CTCGAG 1 cut(s) 659
XmnI GAANNNNTTC 1 cut(s) 497
XspI CTAG 1 cut(s) 461
ZraI GACGTC 1 cut(s) 64
Zsp2I ATGCAT 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.