FvH4_3g09530

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000331
Physical Location & Seq
Forward (+)
5547289 .. 5558139
10851 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g09530.t2

Sequence Viewer

Length: 1041 bp
ATGGACAACAAAACGAACCCGAAAGCCCCGGCCACGAAGCCCAAACCCTCTCCGTCTTCGTCTTCTTCGTCGTCGTCGTCGTCGTCGTCGTCCAGATCCTCCCCCTCCCGCCGCCGAAACTGGCTGGAGCTCCCGCCGGAGATCACCGCTTCGATTATTTCGCGGCTCGGAGCTGTCGAGATCCTGACGGCAGCCGAGAAGGTCTGCAGATCATGGCGCGCAATCTGCAAGGACCCGCTGATGTGGCGCACCATCGATATGCGCAACGACGGCGACCTGCACGACATGACGTACGATTTGGAGAAGATGTGCTGCGACGCCGTTGATCGGAGCTGTGGTCAGCTCGTCGATATCTCCGTCGAGTATTTCGGCAGCGATGAGCTGCTCAAGTATATCACAGCCAGATCAAGTGGAATCAGACGCCTCCGACTAGTGCGGTGTTATTGCATAACAGATGAGGGGTTGAGTGAAGTGGCGCTGAAGCTTCCAATGTTGGAGGACCTTGAACTTTCAATGTGCACACTTTCGCACGAGCCTCTCGAGGTTGTTGGACGCTCTTGTCCGCTTTTGAAATCTCTGAAACTGAACAACCATTGGTACCAGTTCCCGCAGGAGTTGTGTAATAATGATGCATGTGCCGTAGCAGGAACAATGCGAGGTTTGCAGCACCTCCAGCTTTTTGGGAATAAGCTGACAAATGATGGTTTGATAGAACTTCTTGATGGTTGTCCTCGTCTTGAGTCACTTGATCTGCGCCATTGTTTTAATCTTTACATGGATGGAGATTTGGAGAAAAGGTTGACAGAAAACATTAAGAAGTTGTGGCTTCCTTATGATTCCACTGAAGACTATGAGTTTGATGCTATATCTAATGGTTATGAATCACGTGATGATGCTCTGGGATCCCCTTATGGGTTTTCTGGTAGTAATTTGTTGTCTGATGACTATGATGAGTATGGATTTTCAAGTGAAGGTGATTTCTATGACTATGAGGACTATAATGATTTTGCTACACTCAACTTGTATGACTACCCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

347

Amino Acids

39.18

Weight (kDa)

4.67

Isoelectric Point (pI)

64.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 41 - 87 1.8e-11 F-box-like
F-box PF00646 41 - 83 9.4e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000331)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03625 AT4G03630 AT4G05460 AT4G05470 AT4G05475 AT4G05490 AT4G05497
fragaria_vesca FvH4_1g14800 FvH4_2g13770 FvH4_2g19530 FvH4_3g09530 FvH4_3g09530 FvH4_3g09552 FvH4_3g09562 FvH4_3g09640 FvH4_6g48070 FvH4_7g32622 FvH4_7g32650 FvH4_7g32650
malus_domestica MD01G1164600.v1.1 MD05G1060300.v1.1 MD05G1276900.v1.1 MD05G1277000.v1.1 MD13G1280000.v1.1
prunus_persica Prupe.4G087700_v2.0.a1 Prupe.4G087800_v2.0.a1 Prupe.4G087800_v2.0.a1 Prupe.4G087900_v2.0.a1
pyrus_communis pycom05g25780 pycom05g25800
rosa_chinensis RchiOBHm_Chr2g0103761 RchiOBHm_Chr2g0167391 RchiOBHm_Chr5g0015131 RchiOBHm_Chr5g0015151 RchiOBHm_Chr5g0015251 RchiOBHm_Chr5g0015321
rosa_laevigata RLG00000010446 RLG00000017256 RLG00000021713 RLG00000021727 RLG00000032174 RLG00000032175 RLG00000032181 RLG00000032186 RLG00000032190 RLG00000032192
rosa_multiflora Rmu_co8228227.1_g000001 Rmu_co8274851.1_g000001 Rmu_co8371751.1_g000001 Rmu_co8379153.1_g000001 Rmu_co8502117.1_g000002 Rmu_sc0000658.1_g000005 Rmu_sc0000658.1_g000012 Rmu_sc0001840.1_g000005 Rmu_sc0001845.1_g000001 Rmu_sc0002209.1_g000007 Rmu_sc0002209.1_g000013 Rmu_sc0002209.1_g000025 Rmu_sc0024563.1_g000001 Rmu_sc0039033.1_g000001
rosa_roxburghii Rroxscaffold_1G00061130 Rroxscaffold_1G00061210 Rroxscaffold_1G00061260 Rroxscaffold_1G00061390 Rroxscaffold_1G00061400 Rroxscaffold_2G00083550 Rroxscaffold_2G00139420
rosa_rugosa Rorug02G0117000 Rorug02G0530100 Rorug05G0024500 Rorug05G0024500 Rorug05G0025100 Rorug05G0025200 Rorug05G0026000 Rorug05G0026700 Rorug06G0384500
rosa_samantha Rh2AG165900 Rh2AG596900 Rh2AG597000 Rh2BG173200 Rh2BG608400 Rh2CG171900 Rh2CG579200 Rh2DG171400 Rh2DG306300 Rh5AG118900 Rh5AG119200 Rh5AG119300 Rh5AG119600 Rh5AG120000 Rh5AG120100 Rh5BG117000 Rh5BG117100 Rh5BG117200 Rh5BG117600 Rh5BG117700 Rh5BG118200 Rh5BG119000 Rh5CG127900 Rh5CG128000 Rh5CG128700 Rh5CG129500 Rh5DG115800 Rh5DG116000 Rh5DG117000 Rh5DG117400 Rh5DG117900 Rh6BG508700 Rh6BG508800
rosa_wichuraiana Rw2G013010 Rw5G010280 Rw5G010370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 558
Acc16I TGCGCA 1 cut(s) 263
Acc36I ACCTGC 1 cut(s) 285
Acc65I GGTACC 1 cut(s) 597
AccB1I GGYRCC 1 cut(s) 597
AccII CGCG 2 cut(s) 163, 219
AciI CCGC 9 cut(s) 109, 112, 134, 147, 163, 236, 436, 563, 608
AclWI GGATC 4 cut(s) 90, 175, 897, 910
AcoI YGGCCR 1 cut(s) 30
AcuI CTGAAG 2 cut(s) 500, 864
AcvI CACGTG 1 cut(s) 887
AcyI GRCGYC 2 cut(s) 318, 421
AfaI GTAC 2 cut(s) 293, 599
AfiI CCNNNNNNNGG 2 cut(s) 327, 912
AgsI TTSAA 4 cut(s) 506, 513, 571, 966
AhlI ACTAGT 1 cut(s) 430
AluBI AGCT 8 cut(s) 130, 173, 333, 343, 382, 484, 676, 691
AluI AGCT 8 cut(s) 130, 173, 333, 343, 382, 484, 676, 691
Alw21I GWGCWC 2 cut(s) 132, 521
Alw44I GTGCAC 1 cut(s) 517
AlwI GGATC 4 cut(s) 90, 175, 897, 910
Ama87I CYCGRG 1 cut(s) 539
AoxI GGCC 1 cut(s) 30
ApaLI GTGCAC 1 cut(s) 517
ApeKI GCWGC 5 cut(s) 191, 312, 372, 382, 664
ArsI GACNNNNNNTTYG 4 cut(s) 280, 312, 839, 871
Asp718I GGTACC 1 cut(s) 597
AspLEI GCGC 6 cut(s) 219, 221, 249, 264, 478, 756
AspS9I GGNCC 2 cut(s) 232, 499
AsuC2I CCSGG 1 cut(s) 29
AsuHPI GGTGA 2 cut(s) 136, 986
AvaI CYCGRG 1 cut(s) 539
AvaII GGWCC 2 cut(s) 232, 499
BaeGI GKGCMC 1 cut(s) 521
BamHI GGATCC 1 cut(s) 902
BanI GGYRCC 1 cut(s) 597
BanII GRGCYC 1 cut(s) 132
BauI CACGAG 1 cut(s) 530
BbrPI CACGTG 1 cut(s) 887
BbsI GAAGAC 3 cut(s) 48, 54, 852
Bbv12I GWGCWC 2 cut(s) 132, 521
BbvI GCAGC 5 cut(s) 203, 299, 369, 384, 676
BccI CCATC 4 cut(s) 260, 695, 716, 773
BceAI ACGGC 4 cut(s) 204, 286, 305, 623
BcnI CCSGG 1 cut(s) 29
BcuI ACTAGT 1 cut(s) 430
BfaI CTAG 2 cut(s) 431, 1039
BfmI CTRYAG 1 cut(s) 205
BfoI RGCGCY 1 cut(s) 479
BfuAI ACCTGC 1 cut(s) 285
BisI GCNGC 7 cut(s) 112, 164, 192, 313, 373, 383, 665
BlsI GCNGC 7 cut(s) 113, 165, 193, 314, 374, 384, 666
Bme1390I CCNGG 1 cut(s) 29
Bme18I GGWCC 2 cut(s) 232, 499
BmeT110I CYCGRG 1 cut(s) 539
BmgT120I GGNCC 2 cut(s) 232, 499
BmiI GGNNCC 3 cut(s) 234, 599, 904
BmrFI CCNGG 1 cut(s) 29
BmsI GCATC 3 cut(s) 619, 850, 883
BpiI GAAGAC 3 cut(s) 48, 54, 852
BpmI CTGGAG 2 cut(s) 146, 656
BpuEI CTTGAG 2 cut(s) 371, 758
BpuMI CCSGG 1 cut(s) 29
Bsa29I ATCGAT 1 cut(s) 255
BsaAI YACGTR 1 cut(s) 887
BsaHI GRCGYC 2 cut(s) 318, 421
BsaJI CCNNGG 1 cut(s) 27
BsaXI ACNNNNNCTCC 6 cut(s) 293, 322, 323, 352, 782, 812
Bsc4I CCNNNNNNNGG 2 cut(s) 327, 912
Bse1I ACTGG 2 cut(s) 125, 601
BseCI ATCGAT 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 1 cut(s) 784
BseLI CCNNNNNNNGG 2 cut(s) 327, 912
BseNI ACTGG 2 cut(s) 125, 601
BsePI GCGCGC 1 cut(s) 217
BseSI GKGCMC 1 cut(s) 521
BseXI GCAGC 5 cut(s) 203, 299, 369, 384, 676
BsgI GTGCAG 1 cut(s) 263
Bsh1236I CGCG 2 cut(s) 163, 219
BshFI GGCC 1 cut(s) 32
BshNI GGYRCC 1 cut(s) 597
BshVI ATCGAT 1 cut(s) 255
BsiHKAI GWGCWC 2 cut(s) 132, 521
BsiHKCI CYCGRG 1 cut(s) 539
BsiSI CCGG 2 cut(s) 29, 137
BsiWI CGTACG 1 cut(s) 291
BslI CCNNNNNNNGG 2 cut(s) 327, 912
BsnI GGCC 1 cut(s) 32
BsoBI CYCGRG 1 cut(s) 539
Bsp1286I GDGCHC 2 cut(s) 132, 521
Bsp143I GATC 8 cut(s) 95, 141, 180, 209, 325, 404, 748, 902
BspACI CCGC 9 cut(s) 109, 112, 134, 147, 163, 236, 436, 563, 608
BspANI GGCC 1 cut(s) 32
BspDI ATCGAT 1 cut(s) 255
BspFNI CGCG 2 cut(s) 163, 219
BspLI GGNNCC 3 cut(s) 234, 599, 904
BspMAI CTGCAG 1 cut(s) 209
BspMI ACCTGC 1 cut(s) 285
BspPI GGATC 4 cut(s) 90, 175, 897, 910
BspT107I GGYRCC 1 cut(s) 597
BsrI ACTGG 2 cut(s) 125, 601
BssECI CCNNGG 1 cut(s) 27
BssHII GCGCGC 1 cut(s) 217
BssMI GATC 8 cut(s) 95, 141, 180, 209, 325, 404, 748, 902
BssNI GRCGYC 2 cut(s) 318, 421
BssSI CACGAG 1 cut(s) 530
Bst2BI CACGAG 1 cut(s) 530
BstACI GRCGYC 2 cut(s) 318, 421
BstBAI YACGTR 1 cut(s) 887
BstC8I GCNNGC 1 cut(s) 219
BstF5I GGATG 1 cut(s) 784
BstFNI CGCG 2 cut(s) 163, 219
BstH2I RGCGCY 1 cut(s) 479
BstHHI GCGC 6 cut(s) 219, 221, 249, 264, 478, 756
BstKTI GATC 8 cut(s) 98, 144, 183, 212, 328, 407, 751, 905
BstMBI GATC 8 cut(s) 95, 141, 180, 209, 325, 404, 748, 902
BstMWI GCNNNNNNNGC 5 cut(s) 225, 244, 270, 661, 673
BstNSI RCATGY 1 cut(s) 636
BstSCI CCNGG 1 cut(s) 27
BstSFI CTRYAG 1 cut(s) 205
BstSLI GKGCMC 1 cut(s) 521
BstUI CGCG 2 cut(s) 163, 219
BstV1I GCAGC 5 cut(s) 203, 299, 369, 384, 676
BstV2I GAAGAC 3 cut(s) 48, 54, 852
BstX2I RGATCY 3 cut(s) 95, 180, 902
BstXI CCANNNNNNTGG 1 cut(s) 680
BstYI RGATCY 3 cut(s) 95, 180, 902
Bsu15I ATCGAT 1 cut(s) 255
BsuRI GGCC 1 cut(s) 32
BsuTUI ATCGAT 1 cut(s) 255
BtgZI GCGATG 1 cut(s) 390
BtsCI GGATG 1 cut(s) 784
BtsIMutI CAGTG 1 cut(s) 840
BveI ACCTGC 1 cut(s) 285
Cac8I GCNNGC 1 cut(s) 219
CfoI GCGC 6 cut(s) 219, 221, 249, 264, 478, 756
Cfr13I GGNCC 2 cut(s) 232, 499
ClaI ATCGAT 1 cut(s) 255
CseI GACGC 3 cut(s) 326, 429, 561
Csp6I GTAC 2 cut(s) 292, 598
CviAII CATG 4 cut(s) 213, 286, 633, 775
CviQI GTAC 2 cut(s) 292, 598
DpnI GATC 8 cut(s) 97, 143, 182, 211, 327, 406, 750, 904
DpnII GATC 8 cut(s) 95, 141, 180, 209, 325, 404, 748, 902
DrdI GACNNNNNNGTC 1 cut(s) 558
DseDI GACNNNNNNGTC 1 cut(s) 558
EaeI YGGCCR 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 130
Eco24I GRGCYC 1 cut(s) 132
Eco32I GATATC 1 cut(s) 352
Eco47I GGWCC 2 cut(s) 232, 499
Eco53kI GAGCTC 1 cut(s) 130
Eco57I CTGAAG 2 cut(s) 500, 864
Eco72I CACGTG 1 cut(s) 887
Eco88I CYCGRG 1 cut(s) 539
EcoICRI GAGCTC 1 cut(s) 130
EcoO109I RGGNCCY 2 cut(s) 232, 499
EcoRV GATATC 1 cut(s) 352
EcoT22I ATGCAT 1 cut(s) 634
EcoT38I GRGCYC 1 cut(s) 132
FaeI CATG 4 cut(s) 216, 289, 636, 778
FatI CATG 4 cut(s) 212, 285, 632, 774
FauI CCCGC 4 cut(s) 116, 141, 243, 615
Fnu4HI GCNGC 7 cut(s) 112, 164, 192, 313, 373, 383, 665
FokI GGATG 1 cut(s) 791
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 7 cut(s) 112, 164, 192, 313, 373, 383, 665
FspBI CTAG 2 cut(s) 431, 1039
FspI TGCGCA 1 cut(s) 263
GlaI GCGC 6 cut(s) 218, 220, 248, 263, 477, 755
GluI GCNGC 7 cut(s) 112, 164, 192, 313, 373, 383, 665
GsuI CTGGAG 2 cut(s) 146, 656
HaeII RGCGCY 1 cut(s) 479
HaeIII GGCC 1 cut(s) 32
HapII CCGG 2 cut(s) 29, 137
HgaI GACGC 3 cut(s) 326, 429, 561
HhaI GCGC 6 cut(s) 219, 221, 249, 264, 478, 756
Hin1I GRCGYC 2 cut(s) 318, 421
Hin1II CATG 4 cut(s) 216, 289, 636, 778
Hin6I GCGC 6 cut(s) 217, 219, 247, 262, 476, 754
HinP1I GCGC 6 cut(s) 217, 219, 247, 262, 476, 754
HincII GTYRAC 1 cut(s) 801
HindII GTYRAC 1 cut(s) 801
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 4 cut(s) 414, 740, 836, 881
HpaII CCGG 2 cut(s) 29, 137
HphI GGTGA 2 cut(s) 136, 986
Hpy166II GTNNAC 2 cut(s) 519, 801
Hpy188I TCNGA 6 cut(s) 170, 330, 419, 428, 579, 940
Hpy188III TCNNGA 6 cut(s) 93, 178, 184, 539, 719, 737
Hpy8I GTNNAC 2 cut(s) 519, 801
HpyAV CCTTC 2 cut(s) 193, 965
HpyCH4IV ACGT 2 cut(s) 290, 886
HpyCH4V TGCA 7 cut(s) 207, 228, 280, 447, 519, 632, 664
HpyF10VI GCNNNNNNNGC 5 cut(s) 225, 244, 270, 661, 673
HpySE526I ACGT 2 cut(s) 290, 886
Hsp92I GRCGYC 2 cut(s) 318, 421
Hsp92II CATG 4 cut(s) 216, 289, 636, 778
HspAI GCGC 6 cut(s) 217, 219, 247, 262, 476, 754
KpnI GGTACC 1 cut(s) 601
Kzo9I GATC 8 cut(s) 95, 141, 180, 209, 325, 404, 748, 902
LmnI GCTCC 4 cut(s) 127, 135, 170, 330
Lsp1109I GCAGC 5 cut(s) 203, 299, 369, 384, 676
LweI GCATC 3 cut(s) 619, 850, 883
MaeI CTAG 2 cut(s) 431, 1039
MaeII ACGT 2 cut(s) 290, 886
MaeIII GTNAC 1 cut(s) 741
MalI GATC 8 cut(s) 97, 143, 182, 211, 327, 406, 750, 904
MboI GATC 8 cut(s) 95, 141, 180, 209, 325, 404, 748, 902
MboII GAAGA 5 cut(s) 48, 54, 57, 316, 857
MflI RGATCY 3 cut(s) 95, 180, 902
MhlI GDGCHC 2 cut(s) 132, 521
MluCI AATT 1 cut(s) 928
MlyI GAGTC 1 cut(s) 749
MmeI TCCRAC 3 cut(s) 451, 474, 529
Mph1103I ATGCAT 1 cut(s) 634
MseI TTAA 2 cut(s) 765, 813
MslI CAYNNNNRTG 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 238
MspI CCGG 2 cut(s) 29, 137
MspR9I CCNGG 1 cut(s) 29
MvnI CGCG 2 cut(s) 163, 219
MwoI GCNNNNNNNGC 5 cut(s) 225, 244, 270, 661, 673
NciI CCSGG 1 cut(s) 29
NdeII GATC 8 cut(s) 95, 141, 180, 209, 325, 404, 748, 902
NlaIII CATG 4 cut(s) 216, 289, 636, 778
NlaIV GGNNCC 3 cut(s) 234, 599, 904
NmeAIII GCCGAG 1 cut(s) 220
NmuCI GTSAC 1 cut(s) 741
NsbI TGCGCA 1 cut(s) 263
NsiI ATGCAT 1 cut(s) 634
NspI RCATGY 1 cut(s) 636
PaeR7I CTCGAG 1 cut(s) 539
PauI GCGCGC 1 cut(s) 217
PcsI WCGNNNNNNNCGW 9 cut(s) 65, 74, 77, 80, 83, 86, 174, 354, 537
PfeI GAWTC 3 cut(s) 414, 836, 881
Pfl23II CGTACG 1 cut(s) 291
PkrI GCNGC 7 cut(s) 113, 165, 193, 314, 374, 384, 666
PleI GAGTC 1 cut(s) 748
PmaCI CACGTG 1 cut(s) 887
PmlI CACGTG 1 cut(s) 887
PpsI GAGTC 1 cut(s) 748
Ppu21I YACGTR 1 cut(s) 887
PpuMI RGGWCCY 2 cut(s) 232, 499
Psp124BI GAGCTC 1 cut(s) 132
Psp5II RGGWCCY 2 cut(s) 232, 499
PspCI CACGTG 1 cut(s) 887
PspLI CGTACG 1 cut(s) 291
PspN4I GGNNCC 3 cut(s) 234, 599, 904
PspPI GGNCC 2 cut(s) 232, 499
PspPPI RGGWCCY 2 cut(s) 232, 499
PstI CTGCAG 1 cut(s) 209
PsuI RGATCY 3 cut(s) 95, 180, 902
PteI GCGCGC 1 cut(s) 217
RsaI GTAC 2 cut(s) 293, 599
RsaNI GTAC 2 cut(s) 292, 598
RseI CAYNNNNRTG 1 cut(s) 257
SacI GAGCTC 1 cut(s) 132
SaqAI TTAA 2 cut(s) 765, 813
SatI GCNGC 7 cut(s) 112, 164, 192, 313, 373, 383, 665
Sau3AI GATC 8 cut(s) 95, 141, 180, 209, 325, 404, 748, 902
Sau96I GGNCC 2 cut(s) 232, 499
SchI GAGTC 1 cut(s) 749
ScrFI CCNGG 1 cut(s) 29
SduI GDGCHC 2 cut(s) 132, 521
SfaNI GCATC 3 cut(s) 619, 850, 883
SfcI CTRYAG 1 cut(s) 205
Sfr274I CTCGAG 1 cut(s) 539
SinI GGWCC 2 cut(s) 232, 499
SlaI CTCGAG 1 cut(s) 539
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 3 cut(s) 386, 539, 737
SmoI CTYRAG 3 cut(s) 386, 539, 737
SpeI ACTAGT 1 cut(s) 430
Sse9I AATT 1 cut(s) 928
SsiI CCGC 9 cut(s) 109, 112, 134, 147, 163, 236, 436, 563, 608
SspMI CTAG 2 cut(s) 431, 1039
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 27
TaiI ACGT 2 cut(s) 293, 889
TaqI TCGA 6 cut(s) 152, 177, 255, 348, 360, 540
TasI AATT 1 cut(s) 928
TauI GCSGC 2 cut(s) 114, 166
TfiI GAWTC 3 cut(s) 414, 836, 881
Tru1I TTAA 2 cut(s) 765, 813
Tru9I TTAA 2 cut(s) 765, 813
TscAI CASTG 1 cut(s) 847
TseFI GTSAC 1 cut(s) 741
TseI GCWGC 5 cut(s) 191, 312, 372, 382, 664
Tsp45I GTSAC 1 cut(s) 741
TspDTI ATGAA 1 cut(s) 894
TspGWI ACGGA 2 cut(s) 42, 346
TspRI CASTG 1 cut(s) 847
VneI GTGCAC 1 cut(s) 517
VpaK11BI GGWCC 2 cut(s) 232, 499
XceI RCATGY 1 cut(s) 636
XhoI CTCGAG 1 cut(s) 539
XspI CTAG 2 cut(s) 431, 1039
Zsp2I ATGCAT 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.