AT4G05460

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000331
Physical Location & Seq
Reverse (-)
2760502 .. 2762445
1944 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G05460.1

Sequence Viewer

Length: 909 bp
ATGGCTTCTTCGTCTTCACCCCCGGCGGCGATGGAGGTCGGAGAATCTACAAACTGGACGGAGCTTCCACCGGAACTGACTTCAGCAATCCTGCACCGTCTTGGCGCGATTGAGATACTTGAAAACGCTCAGAAAGTTTGCAGATCTTGGCGTCGTGTTTGTAAAGACCCATCGATGTGGCGTAAAATTGACATGCATAACCTCGGAGATTTGGACGACATGGATTACAACCTCGAGATCATGTGCCGTCACGCTGTTGATCGTAGCCAGGGAGGCTTGGTTGATATCGGTATTTGGTACTTTGGTACTGTTGATCTCCTCAATTACATCGCCCATAGGTCGAGTAATCTGAGAAGCCTTAGACTTATAAGGTGTTCTCAAATTACAGACGATGGATTTGTCGAAGCAGTTGTGAAACTTCCACTTGAAGAACTCGAGTTGTCATACTGCTCATTTTCAGTAGAGTCTCTCAGAGTTGTAGGCCAGTGTTGTTTAAATATGAAGACTTTGAAGCTGAACAAGCATCCTCAAAAGGAGAATGACGATGATGCTCTAGCAATCGCTGAAACAATGCCGAAACTTCGCCATCTCCAGCTTTGTGGGAACGGGTTGTCAGACACCGGTTTAAACGCTATTCTTGACAATTGCTCAAACCTGGAACATCTTGATTTACGCCGGTGTTTCAATGTTAACCTTGTTGGGGATTTGCAGAAGCGGTGTTTTGAGAGTGTCAAAGTTGTGAGACACCCTAATGACTCGATTCATGATATCGACATTGGTTCATCAGAAGACGAAGACCCTTATGACTTCTCTGATATCGATCTGATGTCAGGTGATGATGATTTTGAGGGTTACTATGACTTCTCAGGTGCTAGTGACTTTTCTGACTATGACCAGTTTGATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_4 LRR_6

Protein Analysis

302

Amino Acids

34.16

Weight (kDa)

4.56

Isoelectric Point (pI)

49.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 19 - 65 1.5e-11 F-box-like
F-box PF00646 19 - 62 3.5e-07 F-box domain
DUF7885 PF25372 104 - 180 3.7e-06 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000331)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03625 AT4G03630 AT4G05460 AT4G05470 AT4G05475 AT4G05490 AT4G05497
fragaria_vesca FvH4_1g14800 FvH4_2g13770 FvH4_2g19530 FvH4_3g09530 FvH4_3g09530 FvH4_3g09552 FvH4_3g09562 FvH4_3g09640 FvH4_6g48070 FvH4_7g32622 FvH4_7g32650 FvH4_7g32650
malus_domestica MD01G1164600.v1.1 MD05G1060300.v1.1 MD05G1276900.v1.1 MD05G1277000.v1.1 MD13G1280000.v1.1
prunus_persica Prupe.4G087700_v2.0.a1 Prupe.4G087800_v2.0.a1 Prupe.4G087800_v2.0.a1 Prupe.4G087900_v2.0.a1
pyrus_communis pycom05g25780 pycom05g25800
rosa_chinensis RchiOBHm_Chr2g0103761 RchiOBHm_Chr2g0167391 RchiOBHm_Chr5g0015131 RchiOBHm_Chr5g0015151 RchiOBHm_Chr5g0015251 RchiOBHm_Chr5g0015321
rosa_laevigata RLG00000010446 RLG00000017256 RLG00000021713 RLG00000021727 RLG00000032174 RLG00000032175 RLG00000032181 RLG00000032186 RLG00000032190 RLG00000032192
rosa_multiflora Rmu_co8228227.1_g000001 Rmu_co8274851.1_g000001 Rmu_co8371751.1_g000001 Rmu_co8379153.1_g000001 Rmu_co8502117.1_g000002 Rmu_sc0000658.1_g000005 Rmu_sc0000658.1_g000012 Rmu_sc0001840.1_g000005 Rmu_sc0001845.1_g000001 Rmu_sc0002209.1_g000007 Rmu_sc0002209.1_g000013 Rmu_sc0002209.1_g000025 Rmu_sc0024563.1_g000001 Rmu_sc0039033.1_g000001
rosa_roxburghii Rroxscaffold_1G00061130 Rroxscaffold_1G00061210 Rroxscaffold_1G00061260 Rroxscaffold_1G00061390 Rroxscaffold_1G00061400 Rroxscaffold_2G00083550 Rroxscaffold_2G00139420
rosa_rugosa Rorug02G0117000 Rorug02G0530100 Rorug05G0024500 Rorug05G0024500 Rorug05G0025100 Rorug05G0025200 Rorug05G0026000 Rorug05G0026700 Rorug06G0384500
rosa_samantha Rh2AG165900 Rh2AG596900 Rh2AG597000 Rh2BG173200 Rh2BG608400 Rh2CG171900 Rh2CG579200 Rh2DG171400 Rh2DG306300 Rh5AG118900 Rh5AG119200 Rh5AG119300 Rh5AG119600 Rh5AG120000 Rh5AG120100 Rh5BG117000 Rh5BG117100 Rh5BG117200 Rh5BG117600 Rh5BG117700 Rh5BG118200 Rh5BG119000 Rh5CG127900 Rh5CG128000 Rh5CG128700 Rh5CG129500 Rh5DG115800 Rh5DG116000 Rh5DG117000 Rh5DG117400 Rh5DG117900 Rh6BG508700 Rh6BG508800
rosa_wichuraiana Rw2G013010 Rw5G010280 Rw5G010370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 368
AccII CGCG 1 cut(s) 107
AciI CCGC 2 cut(s) 26, 715
AcuI CTGAAG 1 cut(s) 66
AcyI GRCGYC 1 cut(s) 151
AfaI GTAC 2 cut(s) 299, 307
AfiI CCNNNNNNNGG 1 cut(s) 700
AgeI ACCGGT 1 cut(s) 620
AgsI TTSAA 4 cut(s) 122, 428, 511, 685
AjnI CCWGG 2 cut(s) 267, 654
AluBI AGCT 3 cut(s) 64, 514, 595
AluI AGCT 3 cut(s) 64, 514, 595
Alw26I GTCTC 2 cut(s) 471, 736
Ama87I CYCGRG 2 cut(s) 233, 434
AoxI GGCC 1 cut(s) 481
ArsI GACNNNNNNTTYG 2 cut(s) 380, 412
AsiGI ACCGGT 1 cut(s) 620
AspLEI GCGC 1 cut(s) 107
AsuC2I CCSGG 1 cut(s) 23
AsuHPI GGTGA 2 cut(s) 9, 845
AvaI CYCGRG 2 cut(s) 233, 434
BaeI ACNNNNGTAYC 2 cut(s) 289, 322
BbsI GAAGAC 4 cut(s) 6, 509, 795, 801
BccI CCATC 4 cut(s) 25, 178, 386, 594
BceAI ACGGC 1 cut(s) 231
BciT130I CCWGG 2 cut(s) 269, 656
BcnI CCSGG 1 cut(s) 23
BcoDI GTCTC 2 cut(s) 471, 736
BfaI CTAG 3 cut(s) 554, 873, 907
BglI GCCNNNNNGGC 1 cut(s) 273
BglII AGATCT 1 cut(s) 143
BisI GCNGC 1 cut(s) 27
BlsI GCNGC 1 cut(s) 28
Bme1390I CCNGG 3 cut(s) 23, 269, 656
BmeT110I CYCGRG 2 cut(s) 233, 434
BmrFI CCNGG 3 cut(s) 23, 269, 656
BmsI GCATC 2 cut(s) 532, 538
BpiI GAAGAC 4 cut(s) 6, 509, 795, 801
BpmI CTGGAG 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 23
Bsa29I ATCGAT 2 cut(s) 173, 819
BsaBI GATNNNNATC 1 cut(s) 819
BsaHI GRCGYC 1 cut(s) 151
BsaJI CCNNGG 3 cut(s) 21, 202, 268
BsaWI WCCGGW 2 cut(s) 70, 620
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 1 cut(s) 700
Bse118I RCCGGY 2 cut(s) 620, 675
Bse1I ACTGG 3 cut(s) 59, 484, 895
Bse8I GATNNNNATC 1 cut(s) 819
BseBI CCWGG 2 cut(s) 269, 656
BseCI ATCGAT 2 cut(s) 173, 819
BseDI CCNNGG 3 cut(s) 21, 202, 268
BseGI GGATG 1 cut(s) 523
BseJI GATNNNNATC 1 cut(s) 819
BseLI CCNNNNNNNGG 1 cut(s) 700
BseMII CTCAG 4 cut(s) 143, 341, 484, 879
BseNI ACTGG 3 cut(s) 59, 484, 895
BseRI GAGGAG 1 cut(s) 308
BsgI GTGCAG 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 1 cut(s) 483
BshTI ACCGGT 1 cut(s) 620
BshVI ATCGAT 2 cut(s) 173, 819
BsiHKCI CYCGRG 2 cut(s) 233, 434
BsiSI CCGG 4 cut(s) 23, 71, 621, 676
BslI CCNNNNNNNGG 1 cut(s) 700
BsmAI GTCTC 2 cut(s) 471, 736
BsnI GGCC 1 cut(s) 483
BsoBI CYCGRG 2 cut(s) 233, 434
Bsp143I GATC 5 cut(s) 143, 237, 259, 313, 820
BspACI CCGC 2 cut(s) 26, 715
BspANI GGCC 1 cut(s) 483
BspCNI CTCAG 4 cut(s) 142, 342, 483, 878
BspDI ATCGAT 2 cut(s) 173, 819
BspFNI CGCG 1 cut(s) 107
BspHI TCATGA 1 cut(s) 763
BsrFI RCCGGY 2 cut(s) 620, 675
BsrI ACTGG 3 cut(s) 59, 484, 895
BssAI RCCGGY 2 cut(s) 620, 675
BssECI CCNNGG 3 cut(s) 21, 202, 268
BssMI GATC 5 cut(s) 143, 237, 259, 313, 820
BssNI GRCGYC 1 cut(s) 151
Bst2UI CCWGG 2 cut(s) 269, 656
Bst4CI ACNGT 2 cut(s) 98, 310
BstACI GRCGYC 1 cut(s) 151
BstDEI CTNAG 5 cut(s) 129, 350, 359, 470, 865
BstF5I GGATG 1 cut(s) 523
BstFNI CGCG 1 cut(s) 107
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 5 cut(s) 146, 240, 262, 316, 823
BstMAI GTCTC 2 cut(s) 471, 736
BstMBI GATC 5 cut(s) 143, 237, 259, 313, 820
BstMWI GCNNNNNNNGC 2 cut(s) 273, 520
BstNI CCWGG 2 cut(s) 269, 656
BstNSI RCATGY 1 cut(s) 196
BstSCI CCNGG 3 cut(s) 21, 267, 654
BstUI CGCG 1 cut(s) 107
BstV2I GAAGAC 4 cut(s) 6, 509, 795, 801
BstX2I RGATCY 1 cut(s) 143
BstXI CCANNNNNNTGG 2 cut(s) 177, 599
BstYI RGATCY 1 cut(s) 143
Bsu15I ATCGAT 2 cut(s) 173, 819
BsuRI GGCC 1 cut(s) 483
BsuTUI ATCGAT 2 cut(s) 173, 819
BtgZI GCGATG 2 cut(s) 44, 313
BtsCI GGATG 1 cut(s) 523
BtsIMutI CAGTG 1 cut(s) 491
CciI TCATGA 1 cut(s) 763
CfoI GCGC 1 cut(s) 107
Cfr10I RCCGGY 2 cut(s) 620, 675
ClaI ATCGAT 2 cut(s) 173, 819
CseI GACGC 1 cut(s) 140
Csp6I GTAC 2 cut(s) 298, 306
CspAI ACCGGT 1 cut(s) 620
CviAII CATG 4 cut(s) 193, 220, 241, 764
CviJI RGCY 8 cut(s) 5, 64, 267, 276, 357, 483, 514, 595
CviKI_1 RGCY 8 cut(s) 5, 64, 267, 276, 357, 483, 514, 595
CviQI GTAC 2 cut(s) 298, 306
DdeI CTNAG 5 cut(s) 129, 350, 359, 470, 865
DpnI GATC 5 cut(s) 145, 239, 261, 315, 822
DpnII GATC 5 cut(s) 143, 237, 259, 313, 820
DraI TTTAAA 2 cut(s) 495, 627
Eco32I GATATC 3 cut(s) 286, 769, 817
Eco57I CTGAAG 1 cut(s) 66
Eco88I CYCGRG 2 cut(s) 233, 434
EcoRII CCWGG 2 cut(s) 267, 654
EcoRV GATATC 3 cut(s) 286, 769, 817
EcoT22I ATGCAT 1 cut(s) 198
FaeI CATG 4 cut(s) 196, 223, 244, 767
FatI CATG 4 cut(s) 192, 219, 240, 763
Fnu4HI GCNGC 1 cut(s) 27
FokI GGATG 1 cut(s) 510
Fsp4HI GCNGC 1 cut(s) 27
FspBI CTAG 3 cut(s) 554, 873, 907
GlaI GCGC 1 cut(s) 106
GluI GCNGC 1 cut(s) 27
GsuI CTGGAG 1 cut(s) 575
HaeIII GGCC 1 cut(s) 483
HapII CCGG 4 cut(s) 23, 71, 621, 676
HgaI GACGC 1 cut(s) 140
HhaI GCGC 1 cut(s) 107
Hin1I GRCGYC 1 cut(s) 151
Hin1II CATG 4 cut(s) 196, 223, 244, 767
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HinfI GANTC 4 cut(s) 44, 464, 755, 760
HpaI GTTAAC 1 cut(s) 691
HpaII CCGG 4 cut(s) 23, 71, 621, 676
HphI GGTGA 2 cut(s) 9, 845
Hpy166II GTNNAC 1 cut(s) 691
Hpy188III TCNNGA 4 cut(s) 235, 638, 665, 764
Hpy8I GTNNAC 1 cut(s) 691
Hpy99I CGWCG 1 cut(s) 156
HpyCH4III ACNGT 2 cut(s) 98, 310
HpyCH4V TGCA 4 cut(s) 94, 141, 196, 709
HpyF10VI GCNNNNNNNGC 2 cut(s) 273, 520
HpyF3I CTNAG 5 cut(s) 129, 350, 359, 470, 865
Hsp92I GRCGYC 1 cut(s) 151
Hsp92II CATG 4 cut(s) 196, 223, 244, 767
HspAI GCGC 1 cut(s) 105
KspAI GTTAAC 1 cut(s) 691
Kzo9I GATC 5 cut(s) 143, 237, 259, 313, 820
LmnI GCTCC 1 cut(s) 61
LweI GCATC 2 cut(s) 532, 538
MaeI CTAG 3 cut(s) 554, 873, 907
MaeIII GTNAC 3 cut(s) 248, 851, 875
MalI GATC 5 cut(s) 145, 239, 261, 315, 822
MboI GATC 5 cut(s) 143, 237, 259, 313, 820
MboII GAAGA 5 cut(s) 6, 440, 514, 800, 806
MfeI CAATTG 1 cut(s) 643
MflI RGATCY 1 cut(s) 143
MluCI AATT 4 cut(s) 186, 322, 381, 643
MlyI GAGTC 2 cut(s) 473, 749
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 7 cut(s) 28, 212, 242, 266, 329, 537, 841
Mph1103I ATGCAT 1 cut(s) 198
MseI TTAA 3 cut(s) 494, 626, 690
MslI CAYNNNNRTG 2 cut(s) 175, 750
MspI CCGG 4 cut(s) 23, 71, 621, 676
MspR9I CCNGG 3 cut(s) 23, 269, 656
MssI GTTTAAAC 1 cut(s) 627
MunI CAATTG 1 cut(s) 643
MvaI CCWGG 2 cut(s) 269, 656
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 2 cut(s) 273, 520
NciI CCSGG 1 cut(s) 23
NdeII GATC 5 cut(s) 143, 237, 259, 313, 820
NlaIII CATG 4 cut(s) 196, 223, 244, 767
NmuCI GTSAC 2 cut(s) 248, 875
NsiI ATGCAT 1 cut(s) 198
NspI RCATGY 1 cut(s) 196
PaeR7I CTCGAG 2 cut(s) 233, 434
PagI TCATGA 1 cut(s) 763
PfeI GAWTC 2 cut(s) 44, 760
PinAI ACCGGT 1 cut(s) 620
PkrI GCNGC 1 cut(s) 28
PleI GAGTC 2 cut(s) 472, 749
PmeI GTTTAAAC 1 cut(s) 627
PpsI GAGTC 2 cut(s) 472, 749
PsiI TTATAA 1 cut(s) 368
Psp6I CCWGG 2 cut(s) 267, 654
PspGI CCWGG 2 cut(s) 267, 654
PspXI VCTCGAGB 1 cut(s) 434
PsuI RGATCY 1 cut(s) 143
RsaI GTAC 2 cut(s) 299, 307
RsaNI GTAC 2 cut(s) 298, 306
RseI CAYNNNNRTG 2 cut(s) 175, 750
SaqAI TTAA 3 cut(s) 494, 626, 690
SatI GCNGC 1 cut(s) 27
Sau3AI GATC 5 cut(s) 143, 237, 259, 313, 820
SchI GAGTC 2 cut(s) 473, 749
ScrFI CCNGG 3 cut(s) 23, 269, 656
SfaNI GCATC 2 cut(s) 532, 538
Sfr274I CTCGAG 2 cut(s) 233, 434
SgrAI CRCCGGYG 1 cut(s) 675
SlaI CTCGAG 2 cut(s) 233, 434
SmiMI CAYNNNNRTG 2 cut(s) 175, 750
SmlI CTYRAG 2 cut(s) 233, 434
SmoI CTYRAG 2 cut(s) 233, 434
Sse9I AATT 4 cut(s) 186, 322, 381, 643
SsiI CCGC 2 cut(s) 26, 715
SspMI CTAG 3 cut(s) 554, 873, 907
StyD4I CCNGG 3 cut(s) 21, 267, 654
TaaI ACNGT 2 cut(s) 98, 310
TaqI TCGA 8 cut(s) 173, 234, 341, 402, 435, 758, 771, 819
TasI AATT 4 cut(s) 186, 322, 381, 643
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 2 cut(s) 44, 760
Tru1I TTAA 3 cut(s) 494, 626, 690
Tru9I TTAA 3 cut(s) 494, 626, 690
TscAI CASTG 1 cut(s) 491
TseFI GTSAC 2 cut(s) 248, 875
Tsp45I GTSAC 2 cut(s) 248, 875
TspDTI ATGAA 3 cut(s) 515, 752, 771
TspGWI ACGGA 1 cut(s) 74
TspRI CASTG 1 cut(s) 491
XceI RCATGY 1 cut(s) 196
XhoI CTCGAG 2 cut(s) 233, 434
XspI CTAG 3 cut(s) 554, 873, 907
Zsp2I ATGCAT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.