MD02G1161000.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015376
Physical Location & Seq
Forward (+)
13498378 .. 13500849
2472 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1161000.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGCAATGGAGTATTCTTACACTCTGGCCTGCGTAGGGTTTGTTCTTTTGATCGTTTTTAATCTGAGTTATTTGGGAAATGCTGCTGAGAATCAAGGGTTAGGAGCTTCTTTTATCTTTGGTGATTCTCTGGTTGATGCTGGAAACAACAACTATTTGCCCACGTTGTCGAAGGCCAATATGGTTCCGAACGGGATTGATTTCAACGCGTCTGGAGGAAAACCAACCGGAAGGTTTACCAATGGCAGAACCATTGGTGATATTGTTGGAGAAGAATTGGGGCAACCAAAGTATGCACTCCCATATTTGTCACCAGATGCCAAAGGGAAAGCCCTATTGGTTGGGGTGAATTATGCATCAGGGGCAGCAGGGATTATGAATGCAACAGGAAGAATATTTGTTAATAGGGTGGGAATGGATATCCAAGTTGATTTTTTCAACCAGACAAGGATGCAGATCGACAACGTATTGGGTCCATCACAGGCAAAAGAGTACATTATGAAAAGATCCATTTTTTCAGTCACAATTGGGTCCAATGACTTTTTGAACAATTATCTACTACCAGTCCTCTACATTGGAGCAAGAATTTCTCAGAGCCCAGATGCTTTTATTGATGATTTGATCTCTCACTTCAGAGTCCAACTTACTAGACTTTATCAATTGGATGCTCGTAAATTTGTGATTGGAAATGTTGGACCTATTGGATGCATACCTTACCAAAGGACCATAAATCAACTGAATGCAGACCAGTGTGTGGATTTGCCAAATAAGCTAGCTCTTCAGTACAATGGGCGGTTGAAGGGCTTGCTTGCTGAACTGAGTGAGAACCTTCCAGGATCAACATTTGTTTATGCAAACGTGTACGATGTAGTGATGGATCTCATTACAAATTATGACAAATATGGATTTACAACAGCAAGTGTAGCATGCTGTGGAACTGGTGGCCAATACGCAGGTATAATTCCATGTGGTCCACAATCCAGTCTCTGCAAGGATCGGTCGATGCATGTTTTCTGGGATCCCTACCATCCAAGTGAAGCTGCCAACCTTATACTTGCCAAAAAATTGCTTGATGGAGACACAAGAATCATTTCTCCAATGAATCTCAGACAACTCAGAGACCATGGGTTAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

379

Amino Acids

41.17

Weight (kDa)

6.43

Isoelectric Point (pI)

25.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 356 2.2e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23540
fragaria_vesca FvH4_1g14780
malus_domestica MD02G1161000.v1.1 MD15G1275100.v1.1
prunus_persica Prupe.7G142600_v2.0.a1
pyrus_communis pycom02g12710 pycom15g23820
rosa_chinensis RchiOBHm_Chr2g0103741
rosa_laevigata RLG00000017253
rosa_multiflora Rmu_sc0001852.1_g000002
rosa_roxburghii Rroxscaffold_2G00139440
rosa_rugosa Rorug02G0117000
rosa_samantha Rh2AG165800 Rh2BG173100 Rh2CG171800
rosa_wichuraiana Rw2G012990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 944
AccB7I CCANNNNNTGG 1 cut(s) 754
AccII CGCG 1 cut(s) 209
AciI CCGC 1 cut(s) 793
AclWI GGATC 6 cut(s) 501, 844, 885, 1002, 1013, 1026
AcoI YGGCCR 1 cut(s) 943
AcsI RAATTY 2 cut(s) 585, 674
AcuI CTGAAG 2 cut(s) 616, 764
AfaI GTAC 3 cut(s) 494, 785, 863
AfiI CCNNNNNNNGG 2 cut(s) 36, 754
AflIII ACRYGT 2 cut(s) 207, 858
AgsI TTSAA 4 cut(s) 205, 439, 547, 799
AjnI CCWGG 1 cut(s) 832
AjuI GAANNNNNNNTTGG 2 cut(s) 320, 352
AluBI AGCT 4 cut(s) 107, 772, 776, 1040
AluI AGCT 4 cut(s) 107, 772, 776, 1040
Alw26I GTCTC 3 cut(s) 989, 1071, 1113
AlwI GGATC 6 cut(s) 501, 844, 885, 1002, 1013, 1026
AlwNI CAGNNNCTG 1 cut(s) 987
AoxI GGCC 3 cut(s) 27, 174, 943
ApeKI GCWGC 3 cut(s) 83, 365, 1040
ApoI RAATTY 2 cut(s) 585, 674
Asp700I GAANNNNTTC 1 cut(s) 1090
AspS9I GGNCC 5 cut(s) 473, 531, 695, 723, 971
AsuHPI GGTGA 4 cut(s) 134, 269, 303, 358
AsuNHI GCTAGC 1 cut(s) 772
AvaII GGWCC 5 cut(s) 473, 531, 695, 723, 971
BalI TGGCCA 1 cut(s) 945
BamHI GGATCC 1 cut(s) 1018
BanII GRGCYC 1 cut(s) 599
BbvI GCAGC 3 cut(s) 70, 377, 1027
BccI CCATC 4 cut(s) 484, 868, 1035, 1067
BciT130I CCWGG 1 cut(s) 834
BcoDI GTCTC 3 cut(s) 989, 1071, 1113
BfaI CTAG 2 cut(s) 648, 773
BfuAI ACCTGC 1 cut(s) 944
BisI GCNGC 3 cut(s) 84, 366, 1041
BlsI GCNGC 3 cut(s) 85, 367, 1042
Bme1390I CCNGG 1 cut(s) 834
Bme18I GGWCC 5 cut(s) 473, 531, 695, 723, 971
BmgT120I GGNCC 5 cut(s) 473, 531, 695, 723, 971
BmiI GGNNCC 4 cut(s) 186, 474, 532, 1020
BmrFI CCNGG 1 cut(s) 834
BmsI GCATC 8 cut(s) 127, 307, 365, 441, 592, 655, 695, 993
BmtI GCTAGC 1 cut(s) 776
BpmI CTGGAG 1 cut(s) 234
BsaBI GATNNNNATC 1 cut(s) 455
BsaI GGTCTC 1 cut(s) 1113
BsaJI CCNNGG 1 cut(s) 1123
BsaWI WCCGGW 1 cut(s) 227
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 754
Bse1I ACTGG 4 cut(s) 563, 748, 943, 981
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 455
BseBI CCWGG 1 cut(s) 834
BseDI CCNNGG 1 cut(s) 1123
BseGI GGATG 4 cut(s) 456, 670, 710, 1027
BseJI GATNNNNATC 1 cut(s) 455
BseLI CCNNNNNNNGG 2 cut(s) 36, 754
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 6 cut(s) 56, 78, 605, 809, 1120, 1129
BseNI ACTGG 4 cut(s) 563, 748, 943, 981
BseXI GCAGC 3 cut(s) 70, 377, 1027
Bsh1236I CGCG 1 cut(s) 209
Bsh1285I CGRYCG 1 cut(s) 1001
BshFI GGCC 3 cut(s) 29, 176, 945
BsiEI CGRYCG 1 cut(s) 1001
BsiSI CCGG 1 cut(s) 228
BslI CCNNNNNNNGG 2 cut(s) 36, 754
BsmAI GTCTC 3 cut(s) 989, 1071, 1113
BsmI GAATGC 2 cut(s) 385, 745
BsnI GGCC 3 cut(s) 29, 176, 945
Bso31I GGTCTC 1 cut(s) 1113
Bsp1286I GDGCHC 1 cut(s) 599
Bsp143I GATC 8 cut(s) 51, 456, 506, 621, 836, 877, 994, 1018
Bsp19I CCATGG 1 cut(s) 1123
BspACI CCGC 1 cut(s) 793
BspANI GGCC 3 cut(s) 29, 176, 945
BspCNI CTCAG 6 cut(s) 57, 79, 604, 810, 1119, 1128
BspFNI CGCG 1 cut(s) 209
BspLI GGNNCC 4 cut(s) 186, 474, 532, 1020
BspMI ACCTGC 1 cut(s) 944
BspOI GCTAGC 1 cut(s) 776
BspPI GGATC 6 cut(s) 501, 844, 885, 1002, 1013, 1026
BspQI GCTCTTC 1 cut(s) 783
BspTNI GGTCTC 1 cut(s) 1113
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 4 cut(s) 563, 748, 943, 981
BssECI CCNNGG 1 cut(s) 1123
BssMI GATC 8 cut(s) 51, 456, 506, 621, 836, 877, 994, 1018
BssT1I CCWWGG 1 cut(s) 1123
Bst2UI CCWGG 1 cut(s) 834
Bst6I CTCTTC 1 cut(s) 783
BstC8I GCNNGC 5 cut(s) 31, 774, 806, 810, 928
BstDEI CTNAG 6 cut(s) 65, 87, 591, 818, 1106, 1115
BstDSI CCRYGG 1 cut(s) 1123
BstF5I GGATG 4 cut(s) 456, 670, 710, 1027
BstFNI CGCG 1 cut(s) 209
BstKTI GATC 8 cut(s) 54, 459, 509, 624, 839, 880, 997, 1021
BstMAI GTCTC 3 cut(s) 989, 1071, 1113
BstMBI GATC 8 cut(s) 51, 456, 506, 621, 836, 877, 994, 1018
BstMCI CGRYCG 1 cut(s) 1001
BstMWI GCNNNNNNNGC 3 cut(s) 362, 769, 923
BstNI CCWGG 1 cut(s) 834
BstNSI RCATGY 2 cut(s) 930, 1010
BstSCI CCNGG 1 cut(s) 832
BstUI CGCG 1 cut(s) 209
BstV1I GCAGC 3 cut(s) 70, 377, 1027
BstX2I RGATCY 3 cut(s) 506, 877, 1018
BstYI RGATCY 3 cut(s) 506, 877, 1018
BsuRI GGCC 3 cut(s) 29, 176, 945
BtgI CCRYGG 1 cut(s) 1123
BtsCI GGATG 4 cut(s) 456, 670, 710, 1027
BtsIMutI CAGTG 1 cut(s) 755
BveI ACCTGC 1 cut(s) 944
Cac8I GCNNGC 5 cut(s) 31, 774, 806, 810, 928
CaiI CAGNNNCTG 1 cut(s) 987
Cfr13I GGNCC 5 cut(s) 473, 531, 695, 723, 971
CseI GACGC 1 cut(s) 198
Csp6I GTAC 3 cut(s) 493, 784, 862
CviAII CATG 4 cut(s) 927, 966, 1007, 1124
CviQI GTAC 3 cut(s) 493, 784, 862
DdeI CTNAG 6 cut(s) 65, 87, 591, 818, 1106, 1115
DpnI GATC 8 cut(s) 53, 458, 508, 623, 838, 879, 996, 1020
DpnII GATC 8 cut(s) 51, 456, 506, 621, 836, 877, 994, 1018
EaeI YGGCCR 1 cut(s) 943
Eam1104I CTCTTC 1 cut(s) 783
EarI CTCTTC 1 cut(s) 783
Eco130I CCWWGG 1 cut(s) 1123
Eco24I GRGCYC 1 cut(s) 599
Eco31I GGTCTC 1 cut(s) 1113
Eco32I GATATC 1 cut(s) 421
Eco47I GGWCC 5 cut(s) 473, 531, 695, 723, 971
Eco57I CTGAAG 2 cut(s) 616, 764
EcoRII CCWGG 1 cut(s) 832
EcoRV GATATC 1 cut(s) 421
EcoT14I CCWWGG 1 cut(s) 1123
EcoT22I ATGCAT 3 cut(s) 358, 710, 1008
EcoT38I GRGCYC 1 cut(s) 599
ErhI CCWWGG 1 cut(s) 1123
FaeI CATG 4 cut(s) 930, 969, 1010, 1127
FatI CATG 4 cut(s) 926, 965, 1006, 1123
Fnu4HI GCNGC 3 cut(s) 84, 366, 1041
FokI GGATG 4 cut(s) 463, 677, 717, 1014
FriOI GRGCYC 1 cut(s) 599
Fsp4HI GCNGC 3 cut(s) 84, 366, 1041
FspBI CTAG 2 cut(s) 648, 773
GluI GCNGC 3 cut(s) 84, 366, 1041
GsuI CTGGAG 1 cut(s) 234
HaeIII GGCC 3 cut(s) 29, 176, 945
HapII CCGG 1 cut(s) 228
HgaI GACGC 1 cut(s) 198
Hin1II CATG 4 cut(s) 930, 969, 1010, 1127
HinfI GANTC 5 cut(s) 91, 125, 636, 1086, 1102
HpaII CCGG 1 cut(s) 228
HphI GGTGA 4 cut(s) 134, 269, 303, 358
Hpy166II GTNNAC 3 cut(s) 237, 862, 974
Hpy188I TCNGA 6 cut(s) 66, 189, 594, 635, 1109, 1118
Hpy188III TCNNGA 1 cut(s) 213
Hpy8I GTNNAC 3 cut(s) 237, 862, 974
HpyAV CCTTC 4 cut(s) 166, 225, 793, 839
HpyCH4IV ACGT 3 cut(s) 164, 465, 858
HpyCH4V TGCA 9 cut(s) 296, 356, 383, 454, 708, 743, 854, 990, 1006
HpyF10VI GCNNNNNNNGC 3 cut(s) 362, 769, 923
HpyF3I CTNAG 6 cut(s) 65, 87, 591, 818, 1106, 1115
HpySE526I ACGT 3 cut(s) 164, 465, 858
Hsp92II CATG 4 cut(s) 930, 969, 1010, 1127
Kzo9I GATC 8 cut(s) 51, 456, 506, 621, 836, 877, 994, 1018
LguI GCTCTTC 1 cut(s) 783
LmnI GCTCC 2 cut(s) 104, 578
Lsp1109I GCAGC 3 cut(s) 70, 377, 1027
LweI GCATC 8 cut(s) 127, 307, 365, 441, 592, 655, 695, 993
MaeI CTAG 2 cut(s) 648, 773
MaeII ACGT 3 cut(s) 164, 465, 858
MaeIII GTNAC 2 cut(s) 309, 520
MalI GATC 8 cut(s) 53, 458, 508, 623, 838, 879, 996, 1020
MboI GATC 8 cut(s) 51, 456, 506, 621, 836, 877, 994, 1018
MboII GAAGA 3 cut(s) 284, 402, 770
MfeI CAATTG 2 cut(s) 525, 659
MflI RGATCY 3 cut(s) 506, 877, 1018
MhlI GDGCHC 1 cut(s) 599
MlsI TGGCCA 1 cut(s) 945
MluI ACGCGT 1 cut(s) 207
MluNI TGGCCA 1 cut(s) 945
MlyI GAGTC 1 cut(s) 645
MmeI TCCRAC 3 cut(s) 247, 664, 673
MnlI CCTC 2 cut(s) 209, 578
Mox20I TGGCCA 1 cut(s) 945
Mph1103I ATGCAT 3 cut(s) 358, 710, 1008
MroXI GAANNNNTTC 1 cut(s) 1090
MscI TGGCCA 1 cut(s) 945
MseI TTAA 2 cut(s) 60, 402
MslI CAYNNNNRTG 1 cut(s) 1032
Msp20I TGGCCA 1 cut(s) 945
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 1 cut(s) 834
MunI CAATTG 2 cut(s) 525, 659
Mva1269I GAATGC 2 cut(s) 385, 745
MvaI CCWGG 1 cut(s) 834
MvnI CGCG 1 cut(s) 209
MwoI GCNNNNNNNGC 3 cut(s) 362, 769, 923
NcoI CCATGG 1 cut(s) 1123
NdeII GATC 8 cut(s) 51, 456, 506, 621, 836, 877, 994, 1018
NheI GCTAGC 1 cut(s) 772
NlaIII CATG 4 cut(s) 930, 969, 1010, 1127
NlaIV GGNNCC 4 cut(s) 186, 474, 532, 1020
NmuCI GTSAC 2 cut(s) 309, 520
NsiI ATGCAT 3 cut(s) 358, 710, 1008
NspI RCATGY 2 cut(s) 930, 1010
PaeI GCATGC 1 cut(s) 930
PciSI GCTCTTC 1 cut(s) 783
PctI GAATGC 2 cut(s) 385, 745
PdmI GAANNNNTTC 1 cut(s) 1090
PfeI GAWTC 4 cut(s) 91, 125, 1086, 1102
PflMI CCANNNNNTGG 1 cut(s) 754
PfoI TCCNGGA 1 cut(s) 832
PkrI GCNGC 3 cut(s) 85, 367, 1042
PleI GAGTC 1 cut(s) 644
PpsI GAGTC 1 cut(s) 644
Psp6I CCWGG 1 cut(s) 832
PspGI CCWGG 1 cut(s) 832
PspN4I GGNNCC 4 cut(s) 186, 474, 532, 1020
PspPI GGNCC 5 cut(s) 473, 531, 695, 723, 971
PsrI GAACNNNNNNTAC 2 cut(s) 27, 59
PstNI CAGNNNCTG 1 cut(s) 987
PsuI RGATCY 3 cut(s) 506, 877, 1018
RsaI GTAC 3 cut(s) 494, 785, 863
RsaNI GTAC 3 cut(s) 493, 784, 862
RseI CAYNNNNRTG 1 cut(s) 1032
SapI GCTCTTC 1 cut(s) 783
SaqAI TTAA 2 cut(s) 60, 402
SatI GCNGC 3 cut(s) 84, 366, 1041
Sau3AI GATC 8 cut(s) 51, 456, 506, 621, 836, 877, 994, 1018
Sau96I GGNCC 5 cut(s) 473, 531, 695, 723, 971
SchI GAGTC 1 cut(s) 645
ScrFI CCNGG 1 cut(s) 834
SduI GDGCHC 1 cut(s) 599
SfaNI GCATC 8 cut(s) 127, 307, 365, 441, 592, 655, 695, 993
SinI GGWCC 5 cut(s) 473, 531, 695, 723, 971
SmiMI CAYNNNNRTG 1 cut(s) 1032
SphI GCATGC 1 cut(s) 930
SsiI CCGC 1 cut(s) 793
SspI AATATT 1 cut(s) 396
SspMI CTAG 2 cut(s) 648, 773
StyD4I CCNGG 1 cut(s) 832
StyI CCWWGG 1 cut(s) 1123
TaiI ACGT 3 cut(s) 167, 468, 861
TaqI TCGA 3 cut(s) 170, 459, 1001
TaqII GACCGA 1 cut(s) 987
TatI WGTACW 2 cut(s) 492, 783
TfiI GAWTC 4 cut(s) 91, 125, 1086, 1102
Tru1I TTAA 2 cut(s) 60, 402
Tru9I TTAA 2 cut(s) 60, 402
TscAI CASTG 1 cut(s) 755
TseFI GTSAC 2 cut(s) 309, 520
TseI GCWGC 3 cut(s) 83, 365, 1040
Tsp45I GTSAC 2 cut(s) 309, 520
TspDTI ATGAA 3 cut(s) 392, 515, 1115
TspRI CASTG 1 cut(s) 755
Van91I CCANNNNNTGG 1 cut(s) 754
VpaK11BI GGWCC 5 cut(s) 473, 531, 695, 723, 971
XapI RAATTY 2 cut(s) 585, 674
XceI RCATGY 2 cut(s) 930, 1010
XmnI GAANNNNTTC 1 cut(s) 1090
XspI CTAG 2 cut(s) 648, 773
Zsp2I ATGCAT 3 cut(s) 358, 710, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.