AT2G24070

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002767
Physical Location & Seq
Reverse (-)
10231300 .. 10235494
4195 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G24070.1

Sequence Viewer

Length: 1830 bp
ATGCAAGTTGGTTCCAAGAAAGCATCAATGGGGAAACAGCAGCAATCAGTAAGTGACGCTACAAGTCCAAGACCGCCTCTTGCTCCATCTGAGAAGAACAATGTAGGTTCTGTCACGCGCCGTGCTAGAACAATGGAGGTTAGTTCAAGATATAGATCACCGACTCCTACTAAAACTAGGAGATGTCCTTCACCTATTGTCACCAGGACTGCGCCTTCTTCATCACCTGAATCGTTCTTGAAAAGAGCGGTTTCAGCGGAGAGGAACCGTGGTCCTTCGACACCAACTACGCCGGTTAGTGATGTATTGGTAGATTTACCGGTTTCTTCTAGGAGACTATCTACTGGTCGTTTACCTGAAAGCTTATGGCCTTCTACTATGAGAAGCCTCAGTGTTTCTTTTCAGTCTGATTCAGTGTCAGTTCCTGTTAGTAAGAAGGAGAAACCGCTTGTTACTTCTTCGACTGATCGGACATTGAGACCGTCTTCTTCGAATATAGCGCATAAGCAGCAGAGTGAGACAACCTCTGTAACAAGGAAACAAACGCCCGAGAGGAAAAGAAGTCCATTGAAGGGAAAGAATGTGTCTCCTGGTCAATCAGAGAATTCAAAACCTATGGATGGTTCTCATAGTATGTTAATACCACCACAGCATCGATGGTCTGGTCGAATTAGGGGGAACAGAAGCTTTGATCTTGGGGATAAAGCGGTCAGGAGAGTGTCATTGCCTTTGTCCAATAAAAGCTCTAGACATAAGAAGTCTTCTAGTGACATAACAAGGTTGTTTTCATGTTATGATAATGGTAGACTAGAAGTTTCGTCATCAACAACTTCAGAAGATAGTAGTAGCACTGAGTCTCTCAAGCATTTTTCTACAAGTTCATTACCTAGGTTGCATCCAATGTCTGCTCCTGGATCACGTACTGCTTCTCCTAGCAGATCCTCATTTTCATCATCATCATCTTCTAATTCTCGAGGCATGAGTCCTTCAAGAGGAGTGAGTCCTATGAGAGGATTGAGTCCTGTGGGAAACCGCTCTTTGGTGAGATCGTCTACACCGCCATCAAGAGGTGTAAGTCCTTCCCGGATAAGACAAACCGCGCAATCCAGTAGTACCAACACCTCGGTTCTCAGCTTCATTGCGGATGTCAAGAAAGGAAAAAAAGCGACTTACATAGAAGATGTTCATCAACTACGCTTGCTCTATAATCGATATTCGCAGTGGCGGTTTGCAAATGCTCGAGCTGAGGGAGTGAGCTATGTTCAAAGCTTGATTGCTAAGGAAACTCTATACAATGTTTGGCATGCAATATCAGACCTAAGGGATCTAGTGACAACTCAAAGAATTTGTCTCCAACAGTTGAAGCTAGAAATCAAGTTAAGATCCATCTTAAATGATCAGATGGTTTGCCTTGAGGATTGGGCAATGGTTGAGAGAGAACACATTAGTTCTTTAGCTGGAGCCATTGGAGACTTAGAAGCAAATACACTCCGGCTTCCACTAGCCGGAGGAACAAAGGCAGACCTAGGATCTCTGAAGTTAGCTATGTCCTCAGCTCTTGATGTAATGCAGAGTATGGGATCATCCATCTGGTCTTTACATTCGCAGATGGAGGAGATGAACAAACTTGTTTCGGATCTCGCTGTTATAGCTAAAACAGAGAATTTTTTGCTTGACAAATGTGAAAATCTCTTGGCATCAACTGCAGTCATGGAGATAGAAGAGCGTAGCCTCAAGACTCATCTGATACAAAAGAAGCAAGAAGAAGAAGTAAGAGATGATGCAGAGTCTTCTCCATTGTTGCCATTAAGCAAGTTTCAATGGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

609

Amino Acids

66.94

Weight (kDa)

10.2

Isoelectric Point (pI)

72.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 276 - 559 6e-97 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 248, 1035
AccI GTMKAC 2 cut(s) 805, 1052
AccII CGCG 2 cut(s) 118, 1100
AclWI GGATC 7 cut(s) 922, 933, 1332, 1377, 1537, 1588, 1644
AcsI RAATTY 3 cut(s) 604, 1344, 1663
AcuI CTGAAG 2 cut(s) 816, 1556
AfaI GTAC 2 cut(s) 922, 1114
AfiI CCNNNNNNNGG 7 cut(s) 572, 620, 992, 1010, 1039, 1067, 1505
AgeI ACCGGT 1 cut(s) 319
AgsI TTSAA 8 cut(s) 147, 241, 571, 609, 990, 1265, 1363, 1820
AjnI CCWGG 3 cut(s) 203, 589, 910
Alw26I GTCTC 7 cut(s) 328, 472, 512, 591, 861, 1355, 1464
AlwI GGATC 7 cut(s) 922, 933, 1332, 1377, 1537, 1588, 1644
AlwNI CAGNNNCTG 1 cut(s) 425
Ama87I CYCGRG 3 cut(s) 548, 972, 1239
AoxI GGCC 2 cut(s) 368, 1823
ApeKI GCWGC 2 cut(s) 40, 508
ApoI RAATTY 3 cut(s) 604, 1344, 1663
ArsI GACNNNNNNTTYG 2 cut(s) 1333, 1365
AsiGI ACCGGT 1 cut(s) 319
Asp700I GAANNNNTTC 1 cut(s) 1182
AspA2I CCTAGG 2 cut(s) 887, 1525
AspLEI GCGC 4 cut(s) 120, 214, 502, 1102
AspS9I GGNCC 1 cut(s) 272
AsuC2I CCSGG 1 cut(s) 1084
AsuHPI GGTGA 5 cut(s) 150, 183, 193, 216, 1054
AsuII TTCGAA 1 cut(s) 491
AvaI CYCGRG 3 cut(s) 548, 972, 1239
AvaII GGWCC 1 cut(s) 272
AvrII CCTAGG 2 cut(s) 887, 1525
AxyI CCTNAGG 1 cut(s) 1319
BbsI GAAGAC 3 cut(s) 477, 753, 1782
BbvCI CCTCAGC 2 cut(s) 1245, 1552
BbvI GCAGC 2 cut(s) 52, 520
BccI CCATC 8 cut(s) 94, 614, 651, 1069, 1394, 1396, 1595, 1603
BceAI ACGGC 1 cut(s) 105
BciT130I CCWGG 3 cut(s) 205, 591, 912
BclI TGATCA 1 cut(s) 1396
BcnI CCSGG 1 cut(s) 1084
BcoDI GTCTC 7 cut(s) 328, 472, 512, 591, 861, 1355, 1464
BfmI CTRYAG 1 cut(s) 1704
BisI GCNGC 2 cut(s) 41, 509
BlnI CCTAGG 2 cut(s) 887, 1525
BlsI GCNGC 2 cut(s) 42, 510
Bme1390I CCNGG 4 cut(s) 205, 591, 912, 1084
Bme18I GGWCC 1 cut(s) 272
BmeT110I CYCGRG 3 cut(s) 548, 972, 1239
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 3 cut(s) 13, 266, 1462
BmrFI CCNGG 4 cut(s) 205, 591, 912, 1084
BmsI GCATC 5 cut(s) 32, 661, 904, 1706, 1771
BpiI GAAGAC 3 cut(s) 477, 753, 1782
BplI GAGNNNNNCTC 2 cut(s) 509, 541
BpmI CTGGAG 1 cut(s) 1479
Bpu10I CCTNAGC 3 cut(s) 1245, 1278, 1552
Bpu14I TTCGAA 1 cut(s) 491
BpuEI CTTGAG 3 cut(s) 845, 1433, 1718
BpuMI CCSGG 1 cut(s) 1084
Bsa29I ATCGAT 2 cut(s) 655, 1210
BsaAI YACGTR 1 cut(s) 920
BsaBI GATNNNNATC 2 cut(s) 154, 1185
BsaI GGTCTC 1 cut(s) 472
BsaJI CCNNGG 4 cut(s) 268, 887, 1122, 1525
BsaWI WCCGGW 1 cut(s) 319
BsaXI ACNNNNNCTCC 2 cut(s) 913, 943
Bsc4I CCNNNNNNNGG 7 cut(s) 572, 620, 992, 1010, 1039, 1067, 1505
Bse118I RCCGGY 2 cut(s) 292, 319
Bse1I ACTGG 2 cut(s) 349, 1107
Bse21I CCTNAGG 1 cut(s) 1319
Bse3DI GCAATG 3 cut(s) 722, 1137, 1431
Bse8I GATNNNNATC 2 cut(s) 154, 1185
BseBI CCWGG 3 cut(s) 205, 591, 912
BseCI ATCGAT 2 cut(s) 655, 1210
BseDI CCNNGG 4 cut(s) 268, 887, 1122, 1525
BseGI GGATG 4 cut(s) 625, 895, 1150, 1583
BseJI GATNNNNATC 2 cut(s) 154, 1185
BseLI CCNNNNNNNGG 7 cut(s) 572, 620, 992, 1010, 1039, 1067, 1505
BseMI GCAATG 3 cut(s) 722, 1137, 1431
BseMII CTCAG 6 cut(s) 81, 403, 843, 1144, 1236, 1566
BseNI ACTGG 2 cut(s) 349, 1107
BseRI GAGGAG 2 cut(s) 1008, 1628
BseXI GCAGC 2 cut(s) 52, 520
Bsh1236I CGCG 2 cut(s) 118, 1100
BshFI GGCC 2 cut(s) 370, 1825
BshTI ACCGGT 1 cut(s) 319
BshVI ATCGAT 2 cut(s) 655, 1210
BsiHKCI CYCGRG 3 cut(s) 548, 972, 1239
BsiSI CCGG 5 cut(s) 293, 320, 1084, 1492, 1506
BslI CCNNNNNNNGG 7 cut(s) 572, 620, 992, 1010, 1039, 1067, 1505
BsmAI GTCTC 7 cut(s) 328, 472, 512, 591, 861, 1355, 1464
BsnI GGCC 2 cut(s) 370, 1825
Bso31I GGTCTC 1 cut(s) 472
BsoBI CYCGRG 3 cut(s) 548, 972, 1239
Bsp119I TTCGAA 1 cut(s) 491
BspANI GGCC 2 cut(s) 370, 1825
BspCNI CTCAG 6 cut(s) 82, 402, 844, 1143, 1237, 1565
BspDI ATCGAT 2 cut(s) 655, 1210
BspFNI CGCG 2 cut(s) 118, 1100
BspLI GGNNCC 3 cut(s) 13, 266, 1462
BspMAI CTGCAG 1 cut(s) 1708
BspPI GGATC 7 cut(s) 922, 933, 1332, 1377, 1537, 1588, 1644
BspQI GCTCTTC 1 cut(s) 1716
BspT104I TTCGAA 1 cut(s) 491
BspTNI GGTCTC 1 cut(s) 472
BsrBI CCGCTC 2 cut(s) 248, 1035
BsrDI GCAATG 3 cut(s) 722, 1137, 1431
BsrFI RCCGGY 2 cut(s) 292, 319
BsrI ACTGG 2 cut(s) 349, 1107
BssAI RCCGGY 2 cut(s) 292, 319
BssECI CCNNGG 4 cut(s) 268, 887, 1122, 1525
BssT1I CCWWGG 2 cut(s) 887, 1525
Bst2UI CCWGG 3 cut(s) 205, 591, 912
Bst4CI ACNGT 3 cut(s) 269, 483, 1359
Bst6I CTCTTC 1 cut(s) 1716
BstAPI GCANNNNNTGC 1 cut(s) 1703
BstBAI YACGTR 1 cut(s) 920
BstBI TTCGAA 1 cut(s) 491
BstC8I GCNNGC 2 cut(s) 1199, 1305
BstDEI CTNAG 9 cut(s) 90, 389, 852, 1130, 1245, 1278, 1319, 1474, 1552
BstDSI CCRYGG 1 cut(s) 268
BstENI CCTNNNNNAGG 2 cut(s) 990, 1008
BstF5I GGATG 4 cut(s) 625, 895, 1150, 1583
BstFNI CGCG 2 cut(s) 118, 1100
BstHHI GCGC 4 cut(s) 120, 214, 502, 1102
BstMAI GTCTC 7 cut(s) 328, 472, 512, 591, 861, 1355, 1464
BstMWI GCNNNNNNNGC 4 cut(s) 254, 508, 1649, 1703
BstNI CCWGG 3 cut(s) 205, 591, 912
BstNSI RCATGY 1 cut(s) 1307
BstSCI CCNGG 4 cut(s) 203, 589, 910, 1082
BstSFI CTRYAG 1 cut(s) 1704
BstUI CGCG 2 cut(s) 118, 1100
BstV1I GCAGC 2 cut(s) 52, 520
BstV2I GAAGAC 3 cut(s) 477, 753, 1782
BstX2I RGATCY 5 cut(s) 938, 1324, 1382, 1529, 1636
BstYI RGATCY 5 cut(s) 938, 1324, 1382, 1529, 1636
Bsu15I ATCGAT 2 cut(s) 655, 1210
Bsu36I CCTNAGG 1 cut(s) 1319
BsuRI GGCC 2 cut(s) 370, 1825
BsuTUI ATCGAT 2 cut(s) 655, 1210
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 4 cut(s) 625, 895, 1150, 1583
BtsI GCAGTG 1 cut(s) 1226
BtsIMutI CAGTG 4 cut(s) 397, 420, 849, 1226
Cac8I GCNNGC 2 cut(s) 1199, 1305
CaiI CAGNNNCTG 1 cut(s) 425
CfoI GCGC 4 cut(s) 120, 214, 502, 1102
Cfr10I RCCGGY 2 cut(s) 292, 319
Cfr13I GGNCC 1 cut(s) 272
ClaI ATCGAT 2 cut(s) 655, 1210
CseI GACGC 1 cut(s) 65
Csp6I GTAC 2 cut(s) 921, 1113
CspAI ACCGGT 1 cut(s) 319
CviAII CATG 4 cut(s) 789, 979, 1304, 1711
CviQI GTAC 2 cut(s) 921, 1113
DdeI CTNAG 9 cut(s) 90, 389, 852, 1130, 1245, 1278, 1319, 1474, 1552
Eam1104I CTCTTC 1 cut(s) 1716
EarI CTCTTC 1 cut(s) 1716
Eco130I CCWWGG 2 cut(s) 887, 1525
Eco31I GGTCTC 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 272
Eco57I CTGAAG 2 cut(s) 816, 1556
Eco81I CCTNAGG 1 cut(s) 1319
Eco88I CYCGRG 3 cut(s) 548, 972, 1239
EcoNI CCTNNNNNAGG 2 cut(s) 990, 1008
EcoRI GAATTC 1 cut(s) 604
EcoRII CCWGG 3 cut(s) 203, 589, 910
EcoT14I CCWWGG 2 cut(s) 887, 1525
ErhI CCWWGG 2 cut(s) 887, 1525
FaeI CATG 4 cut(s) 792, 982, 1307, 1714
FatI CATG 4 cut(s) 788, 978, 1303, 1710
FbaI TGATCA 1 cut(s) 1396
FblI GTMKAC 2 cut(s) 805, 1052
Fnu4HI GCNGC 2 cut(s) 41, 509
FokI GGATG 4 cut(s) 632, 882, 1157, 1570
Fsp4HI GCNGC 2 cut(s) 41, 509
GlaI GCGC 4 cut(s) 119, 213, 501, 1101
GluI GCNGC 2 cut(s) 41, 509
GsuI CTGGAG 1 cut(s) 1479
HaeIII GGCC 2 cut(s) 370, 1825
HapII CCGG 5 cut(s) 293, 320, 1084, 1492, 1506
HgaI GACGC 1 cut(s) 65
HhaI GCGC 4 cut(s) 120, 214, 502, 1102
Hin1II CATG 4 cut(s) 792, 982, 1307, 1714
Hin6I GCGC 4 cut(s) 118, 212, 500, 1100
HinP1I GCGC 4 cut(s) 118, 212, 500, 1100
HindIII AAGCTT 3 cut(s) 361, 685, 1267
HinfI GANTC 9 cut(s) 163, 230, 410, 854, 982, 1000, 1018, 1738, 1787
HpaII CCGG 5 cut(s) 293, 320, 1084, 1492, 1506
HphI GGTGA 5 cut(s) 150, 183, 193, 216, 1054
Hpy166II GTNNAC 3 cut(s) 353, 806, 1053
Hpy8I GTNNAC 3 cut(s) 353, 806, 1053
HpyAV CCTTC 8 cut(s) 198, 225, 285, 381, 430, 565, 996, 1089
HpyCH4III ACNGT 3 cut(s) 269, 483, 1359
HpyCH4IV ACGT 1 cut(s) 919
HpyCH4V TGCA 7 cut(s) 4, 895, 1232, 1307, 1570, 1706, 1784
HpyF10VI GCNNNNNNNGC 4 cut(s) 254, 508, 1649, 1703
HpyF3I CTNAG 9 cut(s) 90, 389, 852, 1130, 1245, 1278, 1319, 1474, 1552
HpySE526I ACGT 1 cut(s) 919
Hsp92II CATG 4 cut(s) 792, 982, 1307, 1714
HspAI GCGC 4 cut(s) 118, 212, 500, 1100
Ksp22I TGATCA 1 cut(s) 1396
LguI GCTCTTC 1 cut(s) 1716
LmnI GCTCC 3 cut(s) 88, 913, 1460
Lsp1109I GCAGC 2 cut(s) 52, 520
LweI GCATC 5 cut(s) 32, 661, 904, 1706, 1771
MaeII ACGT 1 cut(s) 919
MaeIII GTNAC 7 cut(s) 53, 112, 199, 451, 529, 767, 1330
MbiI CCGCTC 2 cut(s) 248, 1035
MflI RGATCY 5 cut(s) 938, 1324, 1382, 1529, 1636
MluCI AATT 5 cut(s) 604, 669, 967, 1344, 1663
MlyI GAGTC 7 cut(s) 157, 863, 991, 1009, 1027, 1732, 1796
MmeI TCCRAC 1 cut(s) 1378
MroXI GAANNNNTTC 1 cut(s) 1182
MseI TTAA 5 cut(s) 638, 1379, 1391, 1808, 1828
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 5 cut(s) 293, 320, 1084, 1492, 1506
MspR9I CCNGG 4 cut(s) 205, 591, 912, 1084
MvaI CCWGG 3 cut(s) 205, 591, 912
MvnI CGCG 2 cut(s) 118, 1100
MwoI GCNNNNNNNGC 4 cut(s) 254, 508, 1649, 1703
NciI CCSGG 1 cut(s) 1084
NlaIII CATG 4 cut(s) 792, 982, 1307, 1714
NlaIV GGNNCC 3 cut(s) 13, 266, 1462
NmuCI GTSAC 5 cut(s) 53, 112, 199, 767, 1330
NspI RCATGY 1 cut(s) 1307
NspV TTCGAA 1 cut(s) 491
PaeI GCATGC 1 cut(s) 1307
PaeR7I CTCGAG 2 cut(s) 972, 1239
PciSI GCTCTTC 1 cut(s) 1716
PdmI GAANNNNTTC 1 cut(s) 1182
PfeI GAWTC 2 cut(s) 230, 410
PfoI TCCNGGA 2 cut(s) 910, 1082
PinAI ACCGGT 1 cut(s) 319
PkrI GCNGC 2 cut(s) 42, 510
PleI GAGTC 7 cut(s) 157, 862, 990, 1008, 1026, 1732, 1795
PpsI GAGTC 7 cut(s) 157, 862, 990, 1008, 1026, 1732, 1795
Ppu21I YACGTR 1 cut(s) 920
Psp6I CCWGG 3 cut(s) 203, 589, 910
PspGI CCWGG 3 cut(s) 203, 589, 910
PspN4I GGNNCC 3 cut(s) 13, 266, 1462
PspPI GGNCC 1 cut(s) 272
PspXI VCTCGAGB 1 cut(s) 1239
PstI CTGCAG 1 cut(s) 1708
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 5 cut(s) 938, 1324, 1382, 1529, 1636
RsaI GTAC 2 cut(s) 922, 1114
RsaNI GTAC 2 cut(s) 921, 1113
SapI GCTCTTC 1 cut(s) 1716
SaqAI TTAA 5 cut(s) 638, 1379, 1391, 1808, 1828
SatI GCNGC 2 cut(s) 41, 509
Sau96I GGNCC 1 cut(s) 272
SchI GAGTC 7 cut(s) 157, 863, 991, 1009, 1027, 1732, 1796
ScrFI CCNGG 4 cut(s) 205, 591, 912, 1084
SfaNI GCATC 5 cut(s) 32, 661, 904, 1706, 1771
SfcI CTRYAG 1 cut(s) 1704
Sfr274I CTCGAG 2 cut(s) 972, 1239
SfuI TTCGAA 1 cut(s) 491
SinI GGWCC 1 cut(s) 272
SlaI CTCGAG 2 cut(s) 972, 1239
SmlI CTYRAG 5 cut(s) 860, 972, 1239, 1412, 1733
SmoI CTYRAG 5 cut(s) 860, 972, 1239, 1412, 1733
SphI GCATGC 1 cut(s) 1307
Sse9I AATT 5 cut(s) 604, 669, 967, 1344, 1663
StyD4I CCNGG 4 cut(s) 203, 589, 910, 1082
StyI CCWWGG 2 cut(s) 887, 1525
TaaI ACNGT 3 cut(s) 269, 483, 1359
TaiI ACGT 1 cut(s) 922
TaqI TCGA 8 cut(s) 278, 461, 491, 655, 667, 973, 1210, 1240
TasI AATT 5 cut(s) 604, 669, 967, 1344, 1663
TfiI GAWTC 2 cut(s) 230, 410
Tru1I TTAA 5 cut(s) 638, 1379, 1391, 1808, 1828
Tru9I TTAA 5 cut(s) 638, 1379, 1391, 1808, 1828
TscAI CASTG 4 cut(s) 397, 420, 856, 1226
TseFI GTSAC 5 cut(s) 53, 112, 199, 767, 1330
TseI GCWGC 2 cut(s) 40, 508
Tsp45I GTSAC 5 cut(s) 53, 112, 199, 767, 1330
TspDTI ATGAA 7 cut(s) 210, 777, 870, 939, 1126, 1175, 1634
TspRI CASTG 4 cut(s) 397, 420, 856, 1226
VpaK11BI GGWCC 1 cut(s) 272
XagI CCTNNNNNAGG 2 cut(s) 990, 1008
XapI RAATTY 3 cut(s) 604, 1344, 1663
XbaI TCTAGA 1 cut(s) 746
XceI RCATGY 1 cut(s) 1307
XcmI CCANNNNNNNNNTGG 1 cut(s) 654
XhoI CTCGAG 2 cut(s) 972, 1239
XmaJI CCTAGG 2 cut(s) 887, 1525
XmiI GTMKAC 2 cut(s) 805, 1052
XmnI GAANNNNTTC 1 cut(s) 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.