pycom15g13390

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002767
Physical Location & Seq
Forward (+)
9140393 .. 9142056
1664 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g13390.1

Sequence Viewer

Length: 1425 bp
ATGGATGTGCGTGAATCAGCCCTAGCAGCGCTGAGGAAGCAAATAGCCGTGGAGAACCCAAGACTGCCGTTGGTTCCCGCAGGGAGGAACAACAATGCAGTCACTACCCGCCACCCTCGGACGAGAGAAGTTAGTTCTAGGTACAAGTCTCCCACTCCCTCCTCAACAACTCCCTCATCGCCTTCTGGTGCCCGGCGCTGCCCATCGCCAACCCTTACAAGAACATCGCGTCCATCCTCACCGTTGGTGTCCAAGAGATCCCAATCTGTGGATAGGAAGCGGCCTTCCACACCCACTTCCCCACCAAGTCCATCAACGCCCGTCCAGGATTCTTCTGCAGATGTGCAATTGTCGTCCAGAAGAACAGTGAACGGTCGAATGCCAGAGGGTTTATGGCCTTCCACTATGAGGAGTTTGAGTGTTTCCTTCCAATCTGATAGCATTTCAATTCCTGTTAGTAAGAAGGAAAAACCAGTGACGAGTGCTCTGTCTGACCGCACTTTGAGGTCATCTTCGAATGTGGCTCATAGACAGGCTGAAACGCCTGCTGCTCCTCGAAAGCTTACACCAGAGAGAAAAAGGAGCCCTGTTAAGGGGAAAAATGCGCCTAATCAATCAGAGAATTCTAAGCCGGTTGATGCCTTGCATTCCAGATTGATAGATCAGCATCGTTGGCCAAGTAGAATAGGTGGAAAAGTATCTTCCAATTCATTAAATAGAAGTGTAGATCTTGGTGCTAAGATTGTCGGGCTTGCTGCACCAGCTCCTGTAGTTGGGTTGTCTACACTGAGGAGAACACGGCCATCTGATTCTTCGGGTAAACCTTTACAAAAATCCAATAGTGATGCTGCTGCGTTATTATCACTTCATCAAAGTGGTAGAGCAGGACTTGGGGCTTCAGCTGATGGTAATTCCCTGCAGGTATCTGGACCTCACAAGCTTGCCTCGACAAGTTTATCAGGCAGGTTGTCATCAACAACTCATTCACTGAAATCTCAATCTTTGCCTAGTACTCCATCACAATCACGACCATCCTCGCCTAGTAGGAGCTCAATGTTTGCATCCTCTGTTACCAGAGGTTTAAGCCCGTCTCAATCAAGACCATCTACTCCTCCGTCAAGAGGAGTTAGCCCATCTAAGGCAAGGCCATCTGGTACTTCTAGTCAGTCAAGCAGTTCAACTTCTGTGCTTAGTTTCATAGCAGATTTTAAGGGGAAAAAAGGTGCAAGTTGCATTGAAGATGCTCATCAGTTGCGGTTACTTTACAATAGATGTTTGCAATGGCGATTTGCTAATGCAAGGGCAGAGGCTGTACTTTATGTTCAGAAAGTAAATGCAGAGAGAACTTTATTGAATGTGTGGAACACCACACTAAGCTTGTGGGACTCAGTAATCAAGAAACGAATTGATCGATCCAGCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

475

Amino Acids

50.69

Weight (kDa)

11.61

Isoelectric Point (pI)

76.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 264 - 470 1.5e-37 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 910, 954
AccB1I GGYRCC 1 cut(s) 188
AccB7I CCANNNNNTGG 1 cut(s) 268
AccI GTMKAC 1 cut(s) 782
AccII CGCG 1 cut(s) 229
AciI CCGC 5 cut(s) 78, 109, 280, 496, 1255
AclWI GGATC 2 cut(s) 252, 1407
AcoI YGGCCR 2 cut(s) 674, 800
AcsI RAATTY 1 cut(s) 622
AcuI CTGAAG 1 cut(s) 882
AfaI GTAC 4 cut(s) 143, 1012, 1156, 1314
AfeI AGCGCT 1 cut(s) 30
AfiI CCNNNNNNNGG 8 cut(s) 84, 268, 408, 592, 593, 773, 1121, 1138
AgsI TTSAA 4 cut(s) 447, 1179, 1238, 1354
AjnI CCWGG 1 cut(s) 324
AloI GAACNNNNNNTCC 2 cut(s) 214, 246
AluBI AGCT 6 cut(s) 562, 764, 902, 940, 1050, 1377
AluI AGCT 6 cut(s) 562, 764, 902, 940, 1050, 1377
Alw21I GWGCWC 2 cut(s) 487, 1052
Alw26I GTCTC 2 cut(s) 153, 1095
AlwI GGATC 2 cut(s) 252, 1407
AlwNI CAGNNNCTG 2 cut(s) 767, 1310
Aor51HI AGCGCT 1 cut(s) 30
AoxI GGCC 5 cut(s) 281, 395, 674, 800, 1145
ApeKI GCWGC 6 cut(s) 26, 198, 548, 755, 848, 851
ApoI RAATTY 1 cut(s) 622
AspLEI GCGC 3 cut(s) 31, 198, 607
AspS9I GGNCC 1 cut(s) 929
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 1 cut(s) 231
AsuII TTCGAA 1 cut(s) 515
AvaII GGWCC 1 cut(s) 929
BaeGI GKGCMC 1 cut(s) 193
BalI TGGCCA 1 cut(s) 676
BanI GGYRCC 1 cut(s) 188
BanII GRGCYC 2 cut(s) 587, 1052
Bbv12I GWGCWC 2 cut(s) 487, 1052
BbvCI CCTCAGC 1 cut(s) 32
BbvI GCAGC 6 cut(s) 38, 185, 535, 742, 835, 838
BceAI ACGGC 3 cut(s) 32, 52, 815
BciT130I CCWGG 1 cut(s) 326
BcnI CCSGG 1 cut(s) 193
BcoDI GTCTC 2 cut(s) 153, 1095
BfaI CTAG 5 cut(s) 23, 138, 1008, 1041, 1161
BfmI CTRYAG 3 cut(s) 336, 768, 917
BfoI RGCGCY 2 cut(s) 32, 199
BfuAI ACCTGC 2 cut(s) 910, 954
BglII AGATCT 1 cut(s) 727
BisI GCNGC 7 cut(s) 27, 199, 281, 549, 756, 849, 852
BlsI GCNGC 7 cut(s) 28, 200, 282, 550, 757, 850, 853
BmcAI AGTACT 1 cut(s) 1012
Bme1390I CCNGG 2 cut(s) 193, 326
Bme18I GGWCC 1 cut(s) 929
BmgT120I GGNCC 1 cut(s) 929
BmiI GGNNCC 3 cut(s) 75, 190, 584
BmrFI CCNGG 2 cut(s) 193, 326
BmsI GCATC 5 cut(s) 628, 676, 835, 1070, 1231
Bpu10I CCTNAGC 1 cut(s) 32
Bpu14I TTCGAA 1 cut(s) 515
BpuMI CCSGG 1 cut(s) 193
Bsa29I ATCGAT 1 cut(s) 1411
BsaBI GATNNNNATC 3 cut(s) 262, 666, 1245
BsaJI CCNNGG 2 cut(s) 48, 116
BsaXI ACNNNNNCTCC 2 cut(s) 403, 433
Bsc4I CCNNNNNNNGG 8 cut(s) 84, 268, 408, 592, 593, 773, 1121, 1138
Bse118I RCCGGY 1 cut(s) 631
Bse1I ACTGG 1 cut(s) 473
Bse3DI GCAATG 1 cut(s) 1286
Bse8I GATNNNNATC 3 cut(s) 262, 666, 1245
BseBI CCWGG 1 cut(s) 326
BseCI ATCGAT 1 cut(s) 1411
BseDI CCNNGG 2 cut(s) 48, 116
BseGI GGATG 4 cut(s) 10, 233, 1031, 1061
BseJI GATNNNNATC 3 cut(s) 262, 666, 1245
BseLI CCNNNNNNNGG 8 cut(s) 84, 268, 408, 592, 593, 773, 1121, 1138
BseMI GCAATG 1 cut(s) 1286
BseMII CTCAG 3 cut(s) 23, 779, 1401
BseNI ACTGG 1 cut(s) 473
BseRI GAGGAG 6 cut(s) 151, 424, 543, 805, 1101, 1137
BseSI GKGCMC 1 cut(s) 193
BseXI GCAGC 6 cut(s) 38, 185, 535, 742, 835, 838
BsgI GTGCAG 1 cut(s) 741
Bsh1236I CGCG 1 cut(s) 229
Bsh1285I CGRYCG 1 cut(s) 376
BshFI GGCC 5 cut(s) 283, 397, 676, 802, 1147
BshNI GGYRCC 1 cut(s) 188
BshVI ATCGAT 1 cut(s) 1411
BsiEI CGRYCG 1 cut(s) 376
BsiHKAI GWGCWC 2 cut(s) 487, 1052
BsiSI CCGG 2 cut(s) 193, 632
BslFI GGGAC 1 cut(s) 1397
BslI CCNNNNNNNGG 8 cut(s) 84, 268, 408, 592, 593, 773, 1121, 1138
BsmAI GTCTC 2 cut(s) 153, 1095
BsmBI CGTCTC 1 cut(s) 1095
BsmFI GGGAC 1 cut(s) 1397
BsmI GAATGC 2 cut(s) 384, 646
BsnI GGCC 5 cut(s) 283, 397, 676, 802, 1147
Bsp119I TTCGAA 1 cut(s) 515
Bsp1286I GDGCHC 4 cut(s) 193, 487, 587, 1052
Bsp143I GATC 5 cut(s) 257, 661, 727, 1408, 1412
BspACI CCGC 5 cut(s) 78, 109, 280, 496, 1255
BspANI GGCC 5 cut(s) 283, 397, 676, 802, 1147
BspCNI CTCAG 3 cut(s) 24, 780, 1400
BspDI ATCGAT 1 cut(s) 1411
BspFNI CGCG 1 cut(s) 229
BspLI GGNNCC 3 cut(s) 75, 190, 584
BspMAI CTGCAG 2 cut(s) 340, 921
BspMI ACCTGC 2 cut(s) 910, 954
BspPI GGATC 2 cut(s) 252, 1407
BspT104I TTCGAA 1 cut(s) 515
BspT107I GGYRCC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 1286
BsrFI RCCGGY 1 cut(s) 631
BsrI ACTGG 1 cut(s) 473
BssAI RCCGGY 1 cut(s) 631
BssECI CCNNGG 2 cut(s) 48, 116
BssMI GATC 5 cut(s) 257, 661, 727, 1408, 1412
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 3 cut(s) 243, 367, 374
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 3 cut(s) 546, 753, 942
BstDEI CTNAG 8 cut(s) 32, 627, 738, 788, 1137, 1190, 1373, 1387
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 4 cut(s) 10, 233, 1031, 1061
BstFNI CGCG 1 cut(s) 229
BstH2I RGCGCY 2 cut(s) 32, 199
BstHHI GCGC 3 cut(s) 31, 198, 607
BstKTI GATC 5 cut(s) 260, 664, 730, 1411, 1415
BstMAI GTCTC 2 cut(s) 153, 1095
BstMBI GATC 5 cut(s) 257, 661, 727, 1408, 1412
BstMCI CGRYCG 1 cut(s) 376
BstMWI GCNNNNNNNGC 4 cut(s) 26, 37, 673, 761
BstNI CCWGG 1 cut(s) 326
BstSCI CCNGG 2 cut(s) 191, 324
BstSFI CTRYAG 3 cut(s) 336, 768, 917
BstSLI GKGCMC 1 cut(s) 193
BstUI CGCG 1 cut(s) 229
BstV1I GCAGC 6 cut(s) 38, 185, 535, 742, 835, 838
BstX2I RGATCY 2 cut(s) 257, 727
BstYI RGATCY 2 cut(s) 257, 727
Bsu15I ATCGAT 1 cut(s) 1411
BsuRI GGCC 5 cut(s) 283, 397, 676, 802, 1147
BsuTUI ATCGAT 1 cut(s) 1411
BtgI CCRYGG 1 cut(s) 48
BtgZI GCGATG 3 cut(s) 162, 189, 210
BtsCI GGATG 4 cut(s) 10, 233, 1031, 1061
BtsIMutI CAGTG 4 cut(s) 372, 480, 785, 986
BveI ACCTGC 2 cut(s) 910, 954
Cac8I GCNNGC 3 cut(s) 546, 753, 942
CaiI CAGNNNCTG 2 cut(s) 767, 1310
CfoI GCGC 3 cut(s) 31, 198, 607
Cfr10I RCCGGY 1 cut(s) 631
Cfr13I GGNCC 1 cut(s) 929
ClaI ATCGAT 1 cut(s) 1411
CseI GACGC 1 cut(s) 218
Csp6I GTAC 4 cut(s) 142, 1011, 1155, 1313
CviQI GTAC 4 cut(s) 142, 1011, 1155, 1313
DdeI CTNAG 8 cut(s) 32, 627, 738, 788, 1137, 1190, 1373, 1387
DpnI GATC 5 cut(s) 259, 663, 729, 1410, 1414
DpnII GATC 5 cut(s) 257, 661, 727, 1408, 1412
EaeI YGGCCR 2 cut(s) 674, 800
Ecl136II GAGCTC 1 cut(s) 1050
Eco24I GRGCYC 2 cut(s) 587, 1052
Eco47I GGWCC 1 cut(s) 929
Eco47III AGCGCT 1 cut(s) 30
Eco53kI GAGCTC 1 cut(s) 1050
Eco57I CTGAAG 1 cut(s) 882
EcoICRI GAGCTC 1 cut(s) 1050
EcoRI GAATTC 1 cut(s) 622
EcoRII CCWGG 1 cut(s) 324
EcoT38I GRGCYC 2 cut(s) 587, 1052
Esp3I CGTCTC 1 cut(s) 1095
FaiI YATR 5 cut(s) 394, 407, 528, 1199, 1320
FaqI GGGAC 1 cut(s) 1397
FauI CCCGC 2 cut(s) 85, 116
FblI GTMKAC 1 cut(s) 782
Fnu4HI GCNGC 7 cut(s) 27, 199, 281, 549, 756, 849, 852
FokI GGATG 4 cut(s) 17, 220, 1018, 1048
FriOI GRGCYC 2 cut(s) 587, 1052
Fsp4HI GCNGC 7 cut(s) 27, 199, 281, 549, 756, 849, 852
FspBI CTAG 5 cut(s) 23, 138, 1008, 1041, 1161
GlaI GCGC 3 cut(s) 30, 197, 606
GluI GCNGC 7 cut(s) 27, 199, 281, 549, 756, 849, 852
HaeII RGCGCY 2 cut(s) 32, 199
HaeIII GGCC 5 cut(s) 283, 397, 676, 802, 1147
HapII CCGG 2 cut(s) 193, 632
HgaI GACGC 1 cut(s) 218
HhaI GCGC 3 cut(s) 31, 198, 607
Hin6I GCGC 3 cut(s) 29, 196, 605
HinP1I GCGC 3 cut(s) 29, 196, 605
HindIII AAGCTT 3 cut(s) 560, 938, 1375
HinfI GANTC 4 cut(s) 14, 329, 809, 1385
HpaII CCGG 2 cut(s) 193, 632
HphI GGTGA 1 cut(s) 231
Hpy166II GTNNAC 3 cut(s) 370, 783, 821
Hpy188I TCNGA 6 cut(s) 120, 436, 493, 619, 808, 1326
Hpy188III TCNNGA 7 cut(s) 357, 651, 927, 1026, 1098, 1119, 1396
Hpy8I GTNNAC 3 cut(s) 370, 783, 821
HpyAV CCTTC 5 cut(s) 192, 294, 408, 436, 457
HpyCH4III ACNGT 3 cut(s) 243, 367, 374
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 37, 673, 761
HpyF3I CTNAG 8 cut(s) 32, 627, 738, 788, 1137, 1190, 1373, 1387
HspAI GCGC 3 cut(s) 29, 196, 605
Kzo9I GATC 5 cut(s) 257, 661, 727, 1408, 1412
LmnI GCTCC 4 cut(s) 556, 582, 769, 1047
Lsp1109I GCAGC 6 cut(s) 38, 185, 535, 742, 835, 838
LweI GCATC 5 cut(s) 628, 676, 835, 1070, 1231
MaeI CTAG 5 cut(s) 23, 138, 1008, 1041, 1161
MaeIII GTNAC 4 cut(s) 100, 475, 1069, 1257
MalI GATC 5 cut(s) 259, 663, 729, 1410, 1414
MboI GATC 5 cut(s) 257, 661, 727, 1408, 1412
MboII GAAGA 6 cut(s) 324, 372, 504, 693, 804, 1250
MfeI CAATTG 1 cut(s) 347
MflI RGATCY 2 cut(s) 257, 727
MhlI GDGCHC 4 cut(s) 193, 487, 587, 1052
MlsI TGGCCA 1 cut(s) 676
MluCI AATT 6 cut(s) 347, 447, 622, 706, 910, 1404
MluNI TGGCCA 1 cut(s) 676
MlyI GAGTC 1 cut(s) 1379
Mox20I TGGCCA 1 cut(s) 676
MscI TGGCCA 1 cut(s) 676
MseI TTAA 4 cut(s) 591, 713, 1082, 1209
MslI CAYNNNNRTG 1 cut(s) 873
Msp20I TGGCCA 1 cut(s) 676
MspA1I CMGCKG 1 cut(s) 902
MspI CCGG 2 cut(s) 193, 632
MspR9I CCNGG 2 cut(s) 193, 326
MunI CAATTG 1 cut(s) 347
Mva1269I GAATGC 2 cut(s) 384, 646
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 1 cut(s) 229
MwoI GCNNNNNNNGC 4 cut(s) 26, 37, 673, 761
NciI CCSGG 1 cut(s) 193
NdeII GATC 5 cut(s) 257, 661, 727, 1408, 1412
NlaIV GGNNCC 3 cut(s) 75, 190, 584
NmuCI GTSAC 2 cut(s) 100, 475
NspV TTCGAA 1 cut(s) 515
PcsI WCGNNNNNNNCGW 1 cut(s) 1408
PctI GAATGC 2 cut(s) 384, 646
PfeI GAWTC 3 cut(s) 14, 329, 809
PflMI CCANNNNNTGG 1 cut(s) 268
PfoI TCCNGGA 1 cut(s) 324
PkrI GCNGC 7 cut(s) 28, 200, 282, 550, 757, 850, 853
PleI GAGTC 1 cut(s) 1379
PpsI GAGTC 1 cut(s) 1379
Psp124BI GAGCTC 1 cut(s) 1052
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 3 cut(s) 75, 190, 584
PspPI GGNCC 1 cut(s) 929
PsrI GAACNNNNNNTAC 2 cut(s) 1305, 1337
PstI CTGCAG 2 cut(s) 340, 921
PstNI CAGNNNCTG 2 cut(s) 767, 1310
PsuI RGATCY 2 cut(s) 257, 727
PvuII CAGCTG 1 cut(s) 902
RsaI GTAC 4 cut(s) 143, 1012, 1156, 1314
RsaNI GTAC 4 cut(s) 142, 1011, 1155, 1313
RseI CAYNNNNRTG 1 cut(s) 873
SacI GAGCTC 1 cut(s) 1052
SaqAI TTAA 4 cut(s) 591, 713, 1082, 1209
SatI GCNGC 7 cut(s) 27, 199, 281, 549, 756, 849, 852
Sau3AI GATC 5 cut(s) 257, 661, 727, 1408, 1412
Sau96I GGNCC 1 cut(s) 929
SbfI CCTGCAGG 1 cut(s) 921
ScaI AGTACT 1 cut(s) 1012
SchI GAGTC 1 cut(s) 1379
ScrFI CCNGG 2 cut(s) 193, 326
SdaI CCTGCAGG 1 cut(s) 921
SduI GDGCHC 4 cut(s) 193, 487, 587, 1052
SfaNI GCATC 5 cut(s) 628, 676, 835, 1070, 1231
SfcI CTRYAG 3 cut(s) 336, 768, 917
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 1 cut(s) 929
SmiMI CAYNNNNRTG 1 cut(s) 873
Sse8387I CCTGCAGG 1 cut(s) 921
Sse9I AATT 6 cut(s) 347, 447, 622, 706, 910, 1404
SsiI CCGC 5 cut(s) 78, 109, 280, 496, 1255
SspMI CTAG 5 cut(s) 23, 138, 1008, 1041, 1161
SstI GAGCTC 1 cut(s) 1052
StyD4I CCNGG 2 cut(s) 191, 324
TaaI ACNGT 3 cut(s) 243, 367, 374
TaqI TCGA 5 cut(s) 376, 515, 556, 947, 1411
TasI AATT 6 cut(s) 347, 447, 622, 706, 910, 1404
TatI WGTACW 2 cut(s) 1010, 1312
TauI GCSGC 1 cut(s) 283
TfiI GAWTC 3 cut(s) 14, 329, 809
Tru1I TTAA 4 cut(s) 591, 713, 1082, 1209
Tru9I TTAA 4 cut(s) 591, 713, 1082, 1209
TscAI CASTG 4 cut(s) 372, 480, 792, 993
TseFI GTSAC 2 cut(s) 100, 475
TseI GCWGC 6 cut(s) 26, 198, 548, 755, 848, 851
Tsp45I GTSAC 2 cut(s) 100, 475
TspDTI ATGAA 3 cut(s) 699, 857, 1186
TspGWI ACGGA 1 cut(s) 1104
TspRI CASTG 4 cut(s) 372, 480, 792, 993
Van91I CCANNNNNTGG 1 cut(s) 268
VpaK11BI GGWCC 1 cut(s) 929
XapI RAATTY 1 cut(s) 622
XcmI CCANNNNNNNNNTGG 1 cut(s) 390
XmiI GTMKAC 1 cut(s) 782
XspI CTAG 5 cut(s) 23, 138, 1008, 1041, 1161
ZrmI AGTACT 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.