MD02G1005400.v1.1

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002767
Physical Location & Seq
Forward (+)
381079 .. 385304
4226 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1005400.v1.1.491

Sequence Viewer

Length: 1878 bp
ATGGATGTGCGTGAATCAGCTCTAGCAGCGCTGAAGAAGAAAACAGCCGTGGAGACCCCAAGACCGCCGTTGGTTCCGGCGGAGAGGAACAACAATGCAGTCACCACCCGCCGCCCTCGAACGAGGGAAGTTAGTTCTAGGTACAAGTCTCACACTCCATCCTCAACAACTCCCTCATCACCTTCTGGTGCTCGGCGTTGCCCATCTCCAACCCTTACTAGCACCTCACGTCCAACATCGCCATTGGTGTCCAAGAGATCCCAATCTGTTGACAGAAGGCGACCTTCGACACCCACTTCCCCGCCAAGTCCATCAACACCGGTCCGCGATGCAGATGTGCAATTTTCGTTCAGAAGACTAGCAAATGGTCGAATGCCAGAGGGTTTATGGCCTTCCACTATGAGAAGTTTGAGTGTTTCCTTTCAGTCTGATAGCATTTCAATTCCTGTTAGTAGGAAGGAAAAACCAGTGTCGAGTGCTCTGTCTGACCGCACTTTGAGGTCATCTTCGAATGTGGCTCATAGGCAGGCCGAAACACCTGCTGCTCCAAGAAAGCTTACAACGGAGAGAAAAAGGAGCCCTCTTAAAGGCAAAACTGCTCCTGATCAATCAGAGAATTCTAAGCCGGTTGATGGCTTGCATTCCCGATTGATAGATCAGCATCGATGGCCAAGTAGAATAAGTGGGAAAGTATCTTCGAATTCATTAAATAGAAGTGTGGATCTTGGTGCTAAAATTGTCAGGCTTGCTGCGCCAACTCCCGGAGTTGGGTTGTCTACGCTGAGGAGAACGCCACCATCTGATTCTTTGGGCAAATCTTTACAAAAATCCGCCAGTGATACTGCTGCATTATTACCGCTTCATCAAAGCTGTAAAGCAGGACTAGGAGCAAATTCAGTTGATGGTAATTCCCTGCAGGTATCCAGACCTCACAAGCTTGCCTCCACAAGTTTATCGGACAGGTTGTCATTAACATCTCATTCACTGAAATCTCAATCTTCGCTTAGTACTCCATCACAATCACGACCATCCTCGCCTAGTAAGAGCTCAATGTTTTCATCTTCTGTTACAAGAGGTTTAAGCCCGTCTCGATCAAGACCATCTACTCCTCCTTCTAGAGGAGTTAGTCCATCTAAGGCAAGGCCGTCCGGTACTTCTAGTCAATCAAGCAGTTCAACATCTGTGCTTAGTTTCATTGCAGATTTTAAGGGGAAAAAGGGTGCAAGTTACATTGAAGATGCTCATCAGTTGCGTTTACTTTACAATAGATGTTTGCAATGGCGATTTGCCAATGCAAGGGCAGAGGTTGTACTTTATGTTCAGAAAGTAAATGCAGAGAGAACTTTATTGAATGTGTGGAACAGTACAATAAGCTTGTGGGATTCAGTAATCAAGAAACGAATCGATCTCCAGCAGTTGAAGCTTGAACTTAAGCTGAAGTCAGTATTGAATGATCAAATGGCCTACCTTGACGACTGGGGTTTACTTGAAACCGAGCATATTGCTGCATTCTCTGGGGCTATGGAAGATTTGGAGGCAAGCACTCTACGTCTTCCAGTGACTGGAGGGGCAAGGGCAGATTTGGATTCTTTGAAGGTAGCTATCTGCTCAGCGGTTGATGTGATGCAGGCAATGGCATCCTCCATATGCTGTTTGCTCTCACGGGTGGAGGGCGTGAACAGTTTGGCTTCTGAACTTGCTGTTGTAGCAGCACAGGAGAAAGCCATGATTGACGAGTGCGAGGTGCTGTTGGCTTCAGCAGCAGCTATGCAGGTAGAGGAATACAGCCTGAGGACCCATCTCATGCAAACGAAACAAGACTTTGGAAAAGCGAGCGCCCAATTTTGGCAACCAAGACTTGTTCTTGATCCTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

626

Amino Acids

67.66

Weight (kDa)

10.24

Isoelectric Point (pI)

61.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 315 - 578 4.2e-93 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 547
Acc36I ACCTGC 3 cut(s) 547, 907, 1762
AccB7I CCANNNNNTGG 1 cut(s) 1562
AccI GTMKAC 1 cut(s) 776
AccII CGCG 1 cut(s) 327
AclWI GGATC 3 cut(s) 252, 729, 1862
AcoI YGGCCR 1 cut(s) 668
AcsI RAATTY 3 cut(s) 616, 700, 892
AcuI CTGAAG 3 cut(s) 53, 1457, 1740
AfaI GTAC 5 cut(s) 143, 1009, 1153, 1311, 1366
AfeI AGCGCT 1 cut(s) 30
AfiI CCNNNNNNNGG 9 cut(s) 587, 632, 761, 767, 768, 1118, 1296, 1562, 1845
AflII CTTAAG 1 cut(s) 1430
AgeI ACCGGT 1 cut(s) 319
AgsI TTSAA 9 cut(s) 441, 1176, 1235, 1351, 1420, 1427, 1450, 1490, 1594
AhdI GACNNNNNGTC 1 cut(s) 964
AjiI CACGTC 1 cut(s) 230
Alw21I GWGCWC 3 cut(s) 193, 481, 1049
Alw26I GTCTC 3 cut(s) 47, 153, 1092
AlwI GGATC 3 cut(s) 252, 729, 1862
AlwNI CAGNNNCTG 1 cut(s) 1562
Aor51HI AGCGCT 1 cut(s) 30
AoxI GGCC 5 cut(s) 389, 528, 668, 1142, 1461
ApeKI GCWGC 8 cut(s) 26, 542, 749, 845, 1505, 1709, 1760, 1763
ApoI RAATTY 3 cut(s) 616, 700, 892
AsiGI ACCGGT 1 cut(s) 319
AspLEI GCGC 3 cut(s) 31, 754, 1838
AspS9I GGNCC 2 cut(s) 322, 1794
AsuC2I CCSGG 1 cut(s) 762
AsuHPI GGTGA 2 cut(s) 94, 171
AsuII TTCGAA 2 cut(s) 509, 698
AvaII GGWCC 2 cut(s) 322, 1794
AxyI CCTNAGG 1 cut(s) 1790
BalI TGGCCA 1 cut(s) 670
BanII GRGCYC 2 cut(s) 581, 1049
BbsI GAAGAC 2 cut(s) 361, 1544
Bbv12I GWGCWC 3 cut(s) 193, 481, 1049
BbvCI CCTCAGC 1 cut(s) 782
BbvI GCAGC 8 cut(s) 38, 529, 736, 832, 1492, 1721, 1772, 1775
BceAI ACGGC 3 cut(s) 32, 52, 1129
BcgI CGANNNNNNTGC 4 cut(s) 521, 555, 1484, 1518
BciVI GTATCC 1 cut(s) 931
BclI TGATCA 2 cut(s) 604, 1453
BcnI CCSGG 1 cut(s) 762
BcoDI GTCTC 3 cut(s) 47, 153, 1092
BfaI CTAG 9 cut(s) 23, 138, 219, 359, 884, 1038, 1116, 1158, 1876
BfmI CTRYAG 1 cut(s) 914
BfoI RGCGCY 2 cut(s) 32, 1839
BfrI CTTAAG 1 cut(s) 1430
BfuAI ACCTGC 3 cut(s) 547, 907, 1762
BfuI GTATCC 1 cut(s) 931
BisI GCNGC 9 cut(s) 27, 112, 543, 750, 846, 1506, 1710, 1761, 1764
BlpI GCTNAGC 1 cut(s) 1609
BlsI GCNGC 9 cut(s) 28, 113, 544, 751, 847, 1507, 1711, 1762, 1765
BmcAI AGTACT 1 cut(s) 1009
Bme1390I CCNGG 1 cut(s) 762
Bme18I GGWCC 2 cut(s) 322, 1794
BmeRI GACNNNNNGTC 1 cut(s) 964
BmgBI CACGTC 1 cut(s) 230
BmgT120I GGNCC 2 cut(s) 322, 1794
BmiI GGNNCC 3 cut(s) 75, 578, 1796
BmrFI CCNGG 1 cut(s) 762
BmrI ACTGGG 1 cut(s) 1486
BmsI GCATC 5 cut(s) 319, 670, 1228, 1614, 1646
BmuI ACTGGG 1 cut(s) 1486
BpiI GAAGAC 2 cut(s) 361, 1544
BpmI CTGGAG 2 cut(s) 1394, 1584
Bpu10I CCTNAGC 1 cut(s) 782
Bpu1102I GCTNAGC 1 cut(s) 1609
Bpu14I TTCGAA 2 cut(s) 509, 698
BpuMI CCSGG 1 cut(s) 762
Bsa29I ATCGAT 2 cut(s) 664, 1404
BsaBI GATNNNNATC 3 cut(s) 262, 660, 1242
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 1 cut(s) 48
BsaWI WCCGGW 2 cut(s) 319, 1148
Bsc4I CCNNNNNNNGG 9 cut(s) 587, 632, 761, 767, 768, 1118, 1296, 1562, 1845
Bse118I RCCGGY 2 cut(s) 319, 625
Bse1I ACTGG 5 cut(s) 467, 834, 1481, 1556, 1567
Bse21I CCTNAGG 1 cut(s) 1790
Bse3DI GCAATG 3 cut(s) 1194, 1283, 1638
Bse8I GATNNNNATC 3 cut(s) 262, 660, 1242
BseCI ATCGAT 2 cut(s) 664, 1404
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 4 cut(s) 10, 158, 1028, 1637
BseJI GATNNNNATC 3 cut(s) 262, 660, 1242
BseLI CCNNNNNNNGG 9 cut(s) 587, 632, 761, 767, 768, 1118, 1296, 1562, 1845
BseMI GCAATG 3 cut(s) 1194, 1283, 1638
BseMII CTCAG 3 cut(s) 773, 1623, 1781
BseNI ACTGG 5 cut(s) 467, 834, 1481, 1556, 1567
BseRI GAGGAG 3 cut(s) 799, 1098, 1134
BseXI GCAGC 8 cut(s) 38, 529, 736, 832, 1492, 1721, 1772, 1775
Bsh1236I CGCG 1 cut(s) 327
BshFI GGCC 5 cut(s) 391, 530, 670, 1144, 1463
BshTI ACCGGT 1 cut(s) 319
BshVI ATCGAT 2 cut(s) 664, 1404
BsiHKAI GWGCWC 3 cut(s) 193, 481, 1049
BsiSI CCGG 5 cut(s) 77, 320, 626, 762, 1149
BslI CCNNNNNNNGG 9 cut(s) 587, 632, 761, 767, 768, 1118, 1296, 1562, 1845
BsmAI GTCTC 3 cut(s) 47, 153, 1092
BsmBI CGTCTC 1 cut(s) 1092
BsmI GAATGC 3 cut(s) 378, 640, 1508
BsnI GGCC 5 cut(s) 391, 530, 670, 1144, 1463
Bso31I GGTCTC 1 cut(s) 47
Bsp119I TTCGAA 2 cut(s) 509, 698
Bsp1286I GDGCHC 4 cut(s) 193, 481, 581, 1049
Bsp143I GATC 8 cut(s) 257, 604, 655, 721, 1091, 1405, 1453, 1867
Bsp1720I GCTNAGC 1 cut(s) 1609
BspANI GGCC 5 cut(s) 391, 530, 670, 1144, 1463
BspCNI CTCAG 3 cut(s) 774, 1622, 1782
BspDI ATCGAT 2 cut(s) 664, 1404
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 3 cut(s) 75, 578, 1796
BspMAI CTGCAG 1 cut(s) 918
BspMI ACCTGC 3 cut(s) 547, 907, 1762
BspPI GGATC 3 cut(s) 252, 729, 1862
BspT104I TTCGAA 2 cut(s) 509, 698
BspTI CTTAAG 1 cut(s) 1430
BspTNI GGTCTC 1 cut(s) 47
BsrDI GCAATG 3 cut(s) 1194, 1283, 1638
BsrFI RCCGGY 2 cut(s) 319, 625
BsrI ACTGG 5 cut(s) 467, 834, 1481, 1556, 1567
BssAI RCCGGY 2 cut(s) 319, 625
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 8 cut(s) 257, 604, 655, 721, 1091, 1405, 1453, 1867
Bst4CI ACNGT 2 cut(s) 1364, 1682
BstAFI CTTAAG 1 cut(s) 1430
BstBI TTCGAA 2 cut(s) 509, 698
BstC8I GCNNGC 7 cut(s) 528, 638, 747, 939, 1540, 1629, 1834
BstDEI CTNAG 7 cut(s) 621, 782, 1004, 1134, 1187, 1609, 1790
BstDSI CCRYGG 1 cut(s) 48
BstENI CCTNNNNNAGG 2 cut(s) 585, 1116
BstF5I GGATG 4 cut(s) 10, 158, 1028, 1637
BstFNI CGCG 1 cut(s) 327
BstH2I RGCGCY 2 cut(s) 32, 1839
BstHHI GCGC 3 cut(s) 31, 754, 1838
BstKTI GATC 8 cut(s) 260, 607, 658, 724, 1094, 1408, 1456, 1870
BstMAI GTCTC 3 cut(s) 47, 153, 1092
BstMBI GATC 8 cut(s) 257, 604, 655, 721, 1091, 1405, 1453, 1867
BstMWI GCNNNNNNNGC 6 cut(s) 26, 667, 751, 1420, 1706, 1760
BstSCI CCNGG 1 cut(s) 760
BstSFI CTRYAG 1 cut(s) 914
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 8 cut(s) 38, 529, 736, 832, 1492, 1721, 1772, 1775
BstV2I GAAGAC 2 cut(s) 361, 1544
BstX2I RGATCY 2 cut(s) 257, 721
BstYI RGATCY 2 cut(s) 257, 721
Bsu15I ATCGAT 2 cut(s) 664, 1404
Bsu36I CCTNAGG 1 cut(s) 1790
BsuI GTATCC 1 cut(s) 931
BsuRI GGCC 5 cut(s) 391, 530, 670, 1144, 1463
BsuTUI ATCGAT 2 cut(s) 664, 1404
BtgI CCRYGG 1 cut(s) 48
BtgZI GCGATG 2 cut(s) 222, 342
BtrI CACGTC 1 cut(s) 230
BtsCI GGATG 4 cut(s) 10, 158, 1028, 1637
BtsIMutI CAGTG 4 cut(s) 474, 841, 983, 1563
BveI ACCTGC 3 cut(s) 547, 907, 1762
Cac8I GCNNGC 7 cut(s) 528, 638, 747, 939, 1540, 1629, 1834
CaiI CAGNNNCTG 1 cut(s) 1562
CfoI GCGC 3 cut(s) 31, 754, 1838
Cfr10I RCCGGY 2 cut(s) 319, 625
Cfr13I GGNCC 2 cut(s) 322, 1794
ClaI ATCGAT 2 cut(s) 664, 1404
CpoI CGGWCCG 1 cut(s) 322
Csp6I GTAC 5 cut(s) 142, 1008, 1152, 1310, 1365
CspAI ACCGGT 1 cut(s) 319
CspI CGGWCCG 1 cut(s) 322
CviAII CATG 2 cut(s) 1726, 1804
CviQI GTAC 5 cut(s) 142, 1008, 1152, 1310, 1365
DdeI CTNAG 7 cut(s) 621, 782, 1004, 1134, 1187, 1609, 1790
DpnI GATC 8 cut(s) 259, 606, 657, 723, 1093, 1407, 1455, 1869
DpnII GATC 8 cut(s) 257, 604, 655, 721, 1091, 1405, 1453, 1867
DriI GACNNNNNGTC 1 cut(s) 964
EaeI YGGCCR 1 cut(s) 668
Eam1105I GACNNNNNGTC 1 cut(s) 964
EciI GGCGGA 2 cut(s) 95, 820
Ecl136II GAGCTC 1 cut(s) 1047
Eco24I GRGCYC 2 cut(s) 581, 1049
Eco31I GGTCTC 1 cut(s) 47
Eco47I GGWCC 2 cut(s) 322, 1794
Eco47III AGCGCT 1 cut(s) 30
Eco53kI GAGCTC 1 cut(s) 1047
Eco57I CTGAAG 3 cut(s) 53, 1457, 1740
Eco81I CCTNAGG 1 cut(s) 1790
EcoICRI GAGCTC 1 cut(s) 1047
EcoNI CCTNNNNNAGG 2 cut(s) 585, 1116
EcoO109I RGGNCCY 1 cut(s) 1794
EcoRI GAATTC 2 cut(s) 616, 700
EcoT38I GRGCYC 2 cut(s) 581, 1049
Esp3I CGTCTC 1 cut(s) 1092
FaeI CATG 2 cut(s) 1729, 1807
FalI AAGNNNNNCTT 2 cut(s) 268, 300
FatI CATG 2 cut(s) 1725, 1803
FauI CCCGC 2 cut(s) 116, 309
FauNDI CATATG 1 cut(s) 1646
FbaI TGATCA 2 cut(s) 604, 1453
FblI GTMKAC 1 cut(s) 776
Fnu4HI GCNGC 9 cut(s) 27, 112, 543, 750, 846, 1506, 1710, 1761, 1764
FokI GGATG 4 cut(s) 17, 145, 1015, 1624
FriOI GRGCYC 2 cut(s) 581, 1049
Fsp4HI GCNGC 9 cut(s) 27, 112, 543, 750, 846, 1506, 1710, 1761, 1764
FspBI CTAG 9 cut(s) 23, 138, 219, 359, 884, 1038, 1116, 1158, 1876
GlaI GCGC 3 cut(s) 30, 753, 1837
GluI GCNGC 9 cut(s) 27, 112, 543, 750, 846, 1506, 1710, 1761, 1764
GsuI CTGGAG 2 cut(s) 1394, 1584
HaeII RGCGCY 2 cut(s) 32, 1839
HaeIII GGCC 5 cut(s) 391, 530, 670, 1144, 1463
HapII CCGG 5 cut(s) 77, 320, 626, 762, 1149
HhaI GCGC 3 cut(s) 31, 754, 1838
Hin1II CATG 2 cut(s) 1729, 1807
Hin6I GCGC 3 cut(s) 29, 752, 1836
HinP1I GCGC 3 cut(s) 29, 752, 1836
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 4 cut(s) 554, 935, 1372, 1421
HinfI GANTC 5 cut(s) 14, 803, 1382, 1401, 1586
HpaII CCGG 5 cut(s) 77, 320, 626, 762, 1149
HphI GGTGA 2 cut(s) 94, 171
Hpy166II GTNNAC 5 cut(s) 271, 777, 1256, 1484, 1678
Hpy188I TCNGA 8 cut(s) 353, 430, 487, 613, 802, 958, 1323, 1693
Hpy8I GTNNAC 5 cut(s) 271, 777, 1256, 1484, 1678
HpyAV CCTTC 7 cut(s) 192, 270, 294, 402, 451, 1122, 1588
HpyCH4III ACNGT 2 cut(s) 1364, 1682
HpyCH4IV ACGT 2 cut(s) 229, 1549
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 667, 751, 1420, 1706, 1760
HpyF3I CTNAG 7 cut(s) 621, 782, 1004, 1134, 1187, 1609, 1790
HpySE526I ACGT 2 cut(s) 229, 1549
Hsp92II CATG 2 cut(s) 1729, 1807
HspAI GCGC 3 cut(s) 29, 752, 1836
Ksp22I TGATCA 2 cut(s) 604, 1453
Kzo9I GATC 8 cut(s) 257, 604, 655, 721, 1091, 1405, 1453, 1867
LmnI GCTCC 4 cut(s) 550, 576, 604, 887
Lsp1109I GCAGC 8 cut(s) 38, 529, 736, 832, 1492, 1721, 1772, 1775
LweI GCATC 5 cut(s) 319, 670, 1228, 1614, 1646
MaeI CTAG 9 cut(s) 23, 138, 219, 359, 884, 1038, 1116, 1158, 1876
MaeII ACGT 2 cut(s) 229, 1549
MaeIII GTNAC 4 cut(s) 100, 1066, 1226, 1558
MalI GATC 8 cut(s) 259, 606, 657, 723, 1093, 1407, 1455, 1869
MboI GATC 8 cut(s) 257, 604, 655, 721, 1091, 1405, 1453, 1867
MflI RGATCY 2 cut(s) 257, 721
MhlI GDGCHC 4 cut(s) 193, 481, 581, 1049
MlsI TGGCCA 1 cut(s) 670
MluCI AATT 8 cut(s) 341, 441, 616, 700, 735, 892, 907, 1841
MluNI TGGCCA 1 cut(s) 670
MmeI TCCRAC 2 cut(s) 233, 257
Mox20I TGGCCA 1 cut(s) 670
MscI TGGCCA 1 cut(s) 670
MseI TTAA 6 cut(s) 585, 707, 971, 1079, 1206, 1431
Msp20I TGGCCA 1 cut(s) 670
MspA1I CMGCKG 1 cut(s) 1613
MspCI CTTAAG 1 cut(s) 1430
MspI CCGG 5 cut(s) 77, 320, 626, 762, 1149
MspR9I CCNGG 1 cut(s) 762
Mva1269I GAATGC 3 cut(s) 378, 640, 1508
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 6 cut(s) 26, 667, 751, 1420, 1706, 1760
NciI CCSGG 1 cut(s) 762
NdeI CATATG 1 cut(s) 1646
NdeII GATC 8 cut(s) 257, 604, 655, 721, 1091, 1405, 1453, 1867
NlaIII CATG 2 cut(s) 1729, 1807
NlaIV GGNNCC 3 cut(s) 75, 578, 1796
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 2 cut(s) 100, 1558
NspV TTCGAA 2 cut(s) 509, 698
PaqCI CACCTGC 1 cut(s) 547
PctI GAATGC 3 cut(s) 378, 640, 1508
PfeI GAWTC 5 cut(s) 14, 803, 1382, 1401, 1586
PflMI CCANNNNNTGG 1 cut(s) 1562
PfoI TCCNGGA 1 cut(s) 760
PinAI ACCGGT 1 cut(s) 319
PkrI GCNGC 9 cut(s) 28, 113, 544, 751, 847, 1507, 1711, 1762, 1765
PpuMI RGGWCCY 1 cut(s) 1794
Psp124BI GAGCTC 1 cut(s) 1049
Psp5II RGGWCCY 1 cut(s) 1794
PspN4I GGNNCC 3 cut(s) 75, 578, 1796
PspPI GGNCC 2 cut(s) 322, 1794
PspPPI RGGWCCY 1 cut(s) 1794
PsrI GAACNNNNNNTAC 2 cut(s) 1302, 1334
PstI CTGCAG 1 cut(s) 918
PstNI CAGNNNCTG 1 cut(s) 1562
PsuI RGATCY 2 cut(s) 257, 721
RsaI GTAC 5 cut(s) 143, 1009, 1153, 1311, 1366
RsaNI GTAC 5 cut(s) 142, 1008, 1152, 1310, 1365
Rsr2I CGGWCCG 1 cut(s) 322
RsrII CGGWCCG 1 cut(s) 322
SacI GAGCTC 1 cut(s) 1049
SaqAI TTAA 6 cut(s) 585, 707, 971, 1079, 1206, 1431
SatI GCNGC 9 cut(s) 27, 112, 543, 750, 846, 1506, 1710, 1761, 1764
Sau3AI GATC 8 cut(s) 257, 604, 655, 721, 1091, 1405, 1453, 1867
Sau96I GGNCC 2 cut(s) 322, 1794
SbfI CCTGCAGG 1 cut(s) 918
ScaI AGTACT 1 cut(s) 1009
ScrFI CCNGG 1 cut(s) 762
SdaI CCTGCAGG 1 cut(s) 918
SduI GDGCHC 4 cut(s) 193, 481, 581, 1049
SfaNI GCATC 5 cut(s) 319, 670, 1228, 1614, 1646
SfcI CTRYAG 1 cut(s) 914
SfuI TTCGAA 2 cut(s) 509, 698
SinI GGWCC 2 cut(s) 322, 1794
SmlI CTYRAG 1 cut(s) 1430
SmoI CTYRAG 1 cut(s) 1430
Sse8387I CCTGCAGG 1 cut(s) 918
Sse9I AATT 8 cut(s) 341, 441, 616, 700, 735, 892, 907, 1841
SspMI CTAG 9 cut(s) 23, 138, 219, 359, 884, 1038, 1116, 1158, 1876
SstI GAGCTC 1 cut(s) 1049
StyD4I CCNGG 1 cut(s) 760
TaaI ACNGT 2 cut(s) 1364, 1682
TaiI ACGT 2 cut(s) 232, 1552
TaqI TCGA 9 cut(s) 118, 287, 370, 473, 509, 664, 698, 1090, 1404
TasI AATT 8 cut(s) 341, 441, 616, 700, 735, 892, 907, 1841
TatI WGTACW 3 cut(s) 1007, 1309, 1364
TauI GCSGC 1 cut(s) 114
TfiI GAWTC 5 cut(s) 14, 803, 1382, 1401, 1586
Tru1I TTAA 6 cut(s) 585, 707, 971, 1079, 1206, 1431
Tru9I TTAA 6 cut(s) 585, 707, 971, 1079, 1206, 1431
TscAI CASTG 4 cut(s) 474, 841, 990, 1563
TseFI GTSAC 2 cut(s) 100, 1558
TseI GCWGC 8 cut(s) 26, 542, 749, 845, 1505, 1709, 1760, 1763
Tsp45I GTSAC 2 cut(s) 100, 1558
TspDTI ATGAA 4 cut(s) 693, 851, 1047, 1183
TspGWI ACGGA 1 cut(s) 578
TspRI CASTG 4 cut(s) 474, 841, 990, 1563
Van91I CCANNNNNTGG 1 cut(s) 1562
Vha464I CTTAAG 1 cut(s) 1430
VpaK11BI GGWCC 2 cut(s) 322, 1794
XagI CCTNNNNNAGG 2 cut(s) 585, 1116
XapI RAATTY 3 cut(s) 616, 700, 892
XbaI TCTAGA 1 cut(s) 1115
XcmI CCANNNNNNNNNTGG 1 cut(s) 384
XmiI GTMKAC 1 cut(s) 776
XspI CTAG 9 cut(s) 23, 138, 219, 359, 884, 1038, 1116, 1158, 1876
ZrmI AGTACT 1 cut(s) 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.