pycom02g00430

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002767
Physical Location & Seq
Forward (+)
264785 .. 265679
895 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g00430.1

Sequence Viewer

Length: 357 bp
ATGGAAGATTTGGAGGCAAGCACCCTACGTCTTCCAGTGACTGGAGGGGCAAGGGCAGATTTGGATTCTTTGAAGGTAGCTATCTGCTCAGCGGTTGATGTGATGCAGGCAATGGCATCCTCCATATGCTATTTGCTCTCACGGGTGGAGGGCGTGCACAGTTTGGTTTCTGAACTTGCTGTTGTAGCAGCACAGGAGAAAGCCATGCTTGACGAGTGCGAGGCGCTGTTGGCTTCAGCAGCAGCTATGCAGGTAGAGGAATACAGCCTGAGGACCCATCTCTTGCAAACGAAACAAGAAATGGGAAAAGCGAGCGCCCAATTTTGGCAACCAAGACTTGTTCTTGATCCTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

119

Amino Acids

12.74

Weight (kDa)

4.61

Isoelectric Point (pI)

32.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 2 - 71 1.2e-27 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 241
AccB7I CCANNNNNTGG 1 cut(s) 41
AciI CCGC 1 cut(s) 92
AclWI GGATC 1 cut(s) 341
AcuI CTGAAG 1 cut(s) 219
AfiI CCNNNNNNNGG 2 cut(s) 41, 324
AgsI TTSAA 1 cut(s) 73
AluBI AGCT 2 cut(s) 80, 245
AluI AGCT 2 cut(s) 80, 245
Alw21I GWGCWC 1 cut(s) 159
Alw44I GTGCAC 1 cut(s) 155
AlwI GGATC 1 cut(s) 341
AlwNI CAGNNNCTG 1 cut(s) 41
ApaLI GTGCAC 1 cut(s) 155
ApeKI GCWGC 3 cut(s) 188, 239, 242
AspLEI GCGC 2 cut(s) 226, 317
AspS9I GGNCC 1 cut(s) 273
AvaII GGWCC 1 cut(s) 273
AxyI CCTNAGG 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 23
Bbv12I GWGCWC 1 cut(s) 159
BbvI GCAGC 3 cut(s) 200, 251, 254
BccI CCATC 1 cut(s) 285
BfaI CTAG 1 cut(s) 355
BfoI RGCGCY 2 cut(s) 227, 318
BfuAI ACCTGC 1 cut(s) 241
BisI GCNGC 3 cut(s) 189, 240, 243
BlpI GCTNAGC 1 cut(s) 88
BlsI GCNGC 3 cut(s) 190, 241, 244
Bme18I GGWCC 1 cut(s) 273
BmgT120I GGNCC 1 cut(s) 273
BmiI GGNNCC 1 cut(s) 275
BmsI GCATC 2 cut(s) 93, 125
BpiI GAAGAC 1 cut(s) 23
BpmI CTGGAG 1 cut(s) 63
Bpu1102I GCTNAGC 1 cut(s) 88
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 324
Bse1I ACTGG 2 cut(s) 35, 46
Bse21I CCTNAGG 1 cut(s) 269
Bse3DI GCAATG 1 cut(s) 117
BseGI GGATG 1 cut(s) 116
BseLI CCNNNNNNNGG 2 cut(s) 41, 324
BseMI GCAATG 1 cut(s) 117
BseMII CTCAG 2 cut(s) 102, 260
BseNI ACTGG 2 cut(s) 35, 46
BseSI GKGCMC 1 cut(s) 159
BseXI GCAGC 3 cut(s) 200, 251, 254
BsiHKAI GWGCWC 1 cut(s) 159
BslI CCNNNNNNNGG 2 cut(s) 41, 324
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 1 cut(s) 346
Bsp1720I GCTNAGC 1 cut(s) 88
BspACI CCGC 1 cut(s) 92
BspCNI CTCAG 2 cut(s) 101, 261
BspLI GGNNCC 1 cut(s) 275
BspMI ACCTGC 1 cut(s) 241
BspPI GGATC 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 117
BsrI ACTGG 2 cut(s) 35, 46
BssMI GATC 1 cut(s) 346
Bst4CI ACNGT 1 cut(s) 161
BstC8I GCNNGC 4 cut(s) 19, 108, 155, 313
BstDEI CTNAG 2 cut(s) 88, 269
BstF5I GGATG 1 cut(s) 116
BstH2I RGCGCY 2 cut(s) 227, 318
BstHHI GCGC 2 cut(s) 226, 317
BstKTI GATC 1 cut(s) 349
BstMBI GATC 1 cut(s) 346
BstMWI GCNNNNNNNGC 3 cut(s) 185, 230, 239
BstSLI GKGCMC 1 cut(s) 159
BstV1I GCAGC 3 cut(s) 200, 251, 254
BstV2I GAAGAC 1 cut(s) 23
Bsu36I CCTNAGG 1 cut(s) 269
BtsCI GGATG 1 cut(s) 116
BtsIMutI CAGTG 1 cut(s) 42
BveI ACCTGC 1 cut(s) 241
Cac8I GCNNGC 4 cut(s) 19, 108, 155, 313
CaiI CAGNNNCTG 1 cut(s) 41
CfoI GCGC 2 cut(s) 226, 317
Cfr13I GGNCC 1 cut(s) 273
CviAII CATG 1 cut(s) 205
CviJI RGCY 5 cut(s) 80, 203, 233, 245, 267
CviKI_1 RGCY 5 cut(s) 80, 203, 233, 245, 267
DdeI CTNAG 2 cut(s) 88, 269
DpnI GATC 1 cut(s) 348
DpnII GATC 1 cut(s) 346
Eco47I GGWCC 1 cut(s) 273
Eco57I CTGAAG 1 cut(s) 219
Eco81I CCTNAGG 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 273
FaeI CATG 1 cut(s) 208
FaiI YATR 4 cut(s) 125, 127, 206, 248
FalI AAGNNNNNCTT 2 cut(s) 192, 224
FatI CATG 1 cut(s) 204
FauNDI CATATG 1 cut(s) 125
Fnu4HI GCNGC 3 cut(s) 189, 240, 243
FokI GGATG 1 cut(s) 103
Fsp4HI GCNGC 3 cut(s) 189, 240, 243
FspBI CTAG 1 cut(s) 355
GlaI GCGC 2 cut(s) 225, 316
GluI GCNGC 3 cut(s) 189, 240, 243
GsuI CTGGAG 1 cut(s) 63
HaeII RGCGCY 2 cut(s) 227, 318
HhaI GCGC 2 cut(s) 226, 317
Hin1II CATG 1 cut(s) 208
Hin6I GCGC 2 cut(s) 224, 315
HinP1I GCGC 2 cut(s) 224, 315
HinfI GANTC 1 cut(s) 65
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 1 cut(s) 172
Hpy188III TCNNGA 2 cut(s) 344, 350
Hpy8I GTNNAC 1 cut(s) 157
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4IV ACGT 1 cut(s) 28
HpyCH4V TGCA 4 cut(s) 106, 157, 250, 286
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 230, 239
HpyF3I CTNAG 2 cut(s) 88, 269
HpySE526I ACGT 1 cut(s) 28
Hsp92II CATG 1 cut(s) 208
HspAI GCGC 2 cut(s) 224, 315
Kzo9I GATC 1 cut(s) 346
LpnPI CCDG 6 cut(s) 27, 48, 92, 179, 236, 281
Lsp1109I GCAGC 3 cut(s) 200, 251, 254
LweI GCATC 2 cut(s) 93, 125
MaeI CTAG 1 cut(s) 355
MaeII ACGT 1 cut(s) 28
MaeIII GTNAC 1 cut(s) 37
MalI GATC 1 cut(s) 348
MboI GATC 1 cut(s) 346
MboII GAAGA 2 cut(s) 17, 23
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 1 cut(s) 320
MnlI CCTC 7 cut(s) 7, 38, 130, 142, 214, 250, 264
MspA1I CMGCKG 1 cut(s) 92
MwoI GCNNNNNNNGC 3 cut(s) 185, 230, 239
NdeI CATATG 1 cut(s) 125
NdeII GATC 1 cut(s) 346
NlaIII CATG 1 cut(s) 208
NlaIV GGNNCC 1 cut(s) 275
NmuCI GTSAC 1 cut(s) 37
PfeI GAWTC 1 cut(s) 65
PflMI CCANNNNNTGG 1 cut(s) 41
PkrI GCNGC 3 cut(s) 190, 241, 244
PpuMI RGGWCCY 1 cut(s) 273
Psp5II RGGWCCY 1 cut(s) 273
PspN4I GGNNCC 1 cut(s) 275
PspPI GGNCC 1 cut(s) 273
PspPPI RGGWCCY 1 cut(s) 273
PstNI CAGNNNCTG 1 cut(s) 41
SatI GCNGC 3 cut(s) 189, 240, 243
Sau3AI GATC 1 cut(s) 346
Sau96I GGNCC 1 cut(s) 273
SduI GDGCHC 1 cut(s) 159
SetI ASST 5 cut(s) 31, 78, 82, 247, 255
SfaNI GCATC 2 cut(s) 93, 125
SinI GGWCC 1 cut(s) 273
Sse9I AATT 1 cut(s) 320
SsiI CCGC 1 cut(s) 92
SspMI CTAG 1 cut(s) 355
TaaI ACNGT 1 cut(s) 161
TaiI ACGT 1 cut(s) 31
TasI AATT 1 cut(s) 320
TfiI GAWTC 1 cut(s) 65
TscAI CASTG 1 cut(s) 42
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 3 cut(s) 188, 239, 242
Tsp45I GTSAC 1 cut(s) 37
TspRI CASTG 1 cut(s) 42
Van91I CCANNNNNTGG 1 cut(s) 41
VneI GTGCAC 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 273
XspI CTAG 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.