AT2G25200

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013041
Physical Location & Seq
Reverse (-)
10736261 .. 10737800
1540 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25200.1

Sequence Viewer

Length: 1065 bp
ATGAAAATCTCTGCTCTTGGTCGCGGTGGCTCCTCCCCTTTGTCCGCCTGTTTCCATCCTTCCGCCACCGCCATCAACGACGATCTACCGTCGGCTCTTCCATCCCCATCAAATCCATCGTATATCTCCACCTCCGGCGACGCTACCACAACTTGTCAGTACCACACTAACGTTGGTGTTTTCTTTCTTTCATGGTCTCGATCCTTCCTCCGACGCTCTCTCCACCTTCATTTCTACTCATGCAACTCCACAAATTGCTATCTACACTCTCTAGATTGTTACCGTCACTCCATTCCCTTCGCCTTCCGGCTCGAAATCAAACCCTTGACATTCTGGAGAAAAAATGGATCAAAGAAGTTATCTCGAAAGCCGGATATTCGGGTGGTATGGGACCTGACTCACGCAAAATTTGGATCAGGACCCGATCCGGAATCCGGGTTTTACGTGGCAGTCTTTGTATCCGGCGAAGTAGGTCTTCTTGTCGGTGGAGGAAACTTGAAACAGAGACCGAGGAGACAAATCTTGGTGTCCAAGAAAGAGAATCTGTTTGGAAACAGAGTTTACTCTACGAAAATCATGATTCAAGGAAAACTTAGAGAGATTAGTATCGACGTTAAGGTTGTTAACGACGATGCTAGTCTCCGGTTTAGCGTAGACGATAAGAGTGTCCTAAAGATTAGTCAGCTTCAATGGAAATTCAGAGGAAACACAAAGATCGTGATCGATGGTGTGACAATACAGATATCATGGGATGTTTTCAACTGGCTGTTTGGTGGCAAGGATAAGGTTAAACCGGATAAGATTCCCGCCGTGTTCTTACTCCGGTTTGAGAACCAAGAGGTTGAAGGAAACGACGTTTTGATGATGAACAAGCGTGTAAGAGACGATGTCATTTTACGGAAGGAGAATTGTCGAACACCGTCGTTTTGGGCGAGTACGTATAGTCATTGGTCTTCTTCTAGGATGTCTTCTGTGATGGAGTGGTCTAGTTGTAGGGAAGAAGATGAGAGGAGCTTCGGGTCTAAGAGTTGGTTCTCTTTGATTATCTATGCTTGGAGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

354

Amino Acids

40.35

Weight (kDa)

9.63

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 47 - 353 5.3e-66 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013041)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25200 AT5G11000
fragaria_vesca FvH4_2g40820
malus_domestica MD08G1059500.v1.1 MD15G1036800.v1.1
prunus_persica Prupe.1G390900_v2.0.a1
pyrus_communis pycom08g04960 pycom15g03410
rosa_chinensis RchiOBHm_Chr6g0299751
rosa_laevigata RLG00000011391
rosa_multiflora Rmu_sc0002895.1_g000019
rosa_roxburghii Rroxscaffold_2G00123250
rosa_rugosa Rorug06G0292100
rosa_samantha Rh6AG402900 Rh6BG411500 Rh6CG417600 Rh6DG404400
rosa_wichuraiana Rw6G035270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 654
AccII CGCG 1 cut(s) 24
AccIII TCCGGA 1 cut(s) 427
AciI CCGC 5 cut(s) 24, 45, 63, 69, 807
AclI AACGTT 1 cut(s) 171
AclWI GGATC 4 cut(s) 195, 355, 419, 421
AcsI RAATTY 2 cut(s) 407, 695
AfaI GTAC 2 cut(s) 161, 937
AfiI CCNNNNNNNGG 1 cut(s) 434
AgsI TTSAA 5 cut(s) 499, 584, 689, 760, 845
AluBI AGCT 2 cut(s) 685, 1014
AluI AGCT 2 cut(s) 685, 1014
Alw26I GTCTC 5 cut(s) 201, 499, 508, 644, 876
AlwI GGATC 4 cut(s) 195, 355, 419, 421
Aor13HI TCCGGA 1 cut(s) 427
ApoI RAATTY 2 cut(s) 407, 695
AspS9I GGNCC 2 cut(s) 391, 419
AsuC2I CCSGG 1 cut(s) 436
AvaII GGWCC 2 cut(s) 391, 419
BbsI GAAGAC 3 cut(s) 467, 945, 960
BccI CCATC 7 cut(s) 63, 80, 109, 115, 124, 719, 970
BceAI ACGGC 1 cut(s) 794
BciVI GTATCC 1 cut(s) 469
BcnI CCSGG 1 cut(s) 436
BcoDI GTCTC 5 cut(s) 201, 499, 508, 644, 876
BfaI CTAG 4 cut(s) 272, 636, 960, 987
BfuI GTATCC 1 cut(s) 469
Bme1390I CCNGG 1 cut(s) 436
Bme18I GGWCC 2 cut(s) 391, 419
BmgT120I GGNCC 2 cut(s) 391, 419
BmiI GGNNCC 3 cut(s) 31, 392, 421
BmrFI CCNGG 1 cut(s) 436
BmsI GCATC 1 cut(s) 622
BpiI GAAGAC 3 cut(s) 467, 945, 960
BpmI CTGGAG 1 cut(s) 355
BpuMI CCSGG 1 cut(s) 436
Bsa29I ATCGAT 1 cut(s) 723
BsaAI YACGTR 2 cut(s) 445, 939
BsaBI GATNNNNATC 2 cut(s) 605, 719
BsaI GGTCTC 2 cut(s) 201, 499
BsaJI CCNNGG 1 cut(s) 509
BsaWI WCCGGW 4 cut(s) 427, 642, 793, 822
BsaXI ACNNNNNCTCC 4 cut(s) 204, 234, 272, 302
Bsc4I CCNNNNNNNGG 1 cut(s) 434
Bse1I ACTGG 1 cut(s) 767
Bse8I GATNNNNATC 2 cut(s) 605, 719
BseAI TCCGGA 1 cut(s) 427
BseCI ATCGAT 1 cut(s) 723
BseDI CCNNGG 1 cut(s) 509
BseGI GGATG 4 cut(s) 55, 101, 757, 969
BseJI GATNNNNATC 2 cut(s) 605, 719
BseLI CCNNNNNNNGG 1 cut(s) 434
BseNI ACTGG 1 cut(s) 767
BseRI GAGGAG 3 cut(s) 22, 526, 1024
Bsh1236I CGCG 1 cut(s) 24
BshVI ATCGAT 1 cut(s) 723
BsiSI CCGG 9 cut(s) 135, 307, 371, 428, 435, 462, 643, 794, 823
BslFI GGGAC 1 cut(s) 404
BslI CCNNNNNNNGG 1 cut(s) 434
BsmAI GTCTC 5 cut(s) 201, 499, 508, 644, 876
BsmBI CGTCTC 1 cut(s) 876
BsmFI GGGAC 1 cut(s) 404
Bso31I GGTCTC 2 cut(s) 201, 499
Bsp13I TCCGGA 1 cut(s) 427
Bsp143I GATC 7 cut(s) 82, 200, 347, 413, 424, 714, 720
BspACI CCGC 5 cut(s) 24, 45, 63, 69, 807
BspDI ATCGAT 1 cut(s) 723
BspEI TCCGGA 1 cut(s) 427
BspFNI CGCG 1 cut(s) 24
BspHI TCATGA 1 cut(s) 576
BspLI GGNNCC 3 cut(s) 31, 392, 421
BspPI GGATC 4 cut(s) 195, 355, 419, 421
BspQI GCTCTTC 1 cut(s) 102
BspTNI GGTCTC 2 cut(s) 201, 499
BsrI ACTGG 1 cut(s) 767
BssECI CCNNGG 1 cut(s) 509
BssMI GATC 7 cut(s) 82, 200, 347, 413, 424, 714, 720
Bst4CI ACNGT 3 cut(s) 90, 284, 921
Bst6I CTCTTC 1 cut(s) 102
BstBAI YACGTR 2 cut(s) 445, 939
BstDEI CTNAG 2 cut(s) 593, 1023
BstF5I GGATG 4 cut(s) 55, 101, 757, 969
BstFNI CGCG 1 cut(s) 24
BstKTI GATC 7 cut(s) 85, 203, 350, 416, 427, 717, 723
BstMAI GTCTC 5 cut(s) 201, 499, 508, 644, 876
BstMBI GATC 7 cut(s) 82, 200, 347, 413, 424, 714, 720
BstSCI CCNGG 1 cut(s) 434
BstSNI TACGTA 1 cut(s) 939
BstUI CGCG 1 cut(s) 24
BstV2I GAAGAC 3 cut(s) 467, 945, 960
Bsu15I ATCGAT 1 cut(s) 723
BsuI GTATCC 1 cut(s) 469
BsuTUI ATCGAT 1 cut(s) 723
BtsCI GGATG 4 cut(s) 55, 101, 757, 969
CciI TCATGA 1 cut(s) 576
Cfr13I GGNCC 2 cut(s) 391, 419
ClaI ATCGAT 1 cut(s) 723
CseI GACGC 2 cut(s) 149, 222
Csp6I GTAC 2 cut(s) 160, 936
CviAII CATG 4 cut(s) 192, 240, 577, 747
CviJI RGCY 7 cut(s) 30, 95, 310, 370, 685, 766, 1014
CviKI_1 RGCY 7 cut(s) 30, 95, 310, 370, 685, 766, 1014
CviQI GTAC 2 cut(s) 160, 936
DdeI CTNAG 2 cut(s) 593, 1023
DpnI GATC 7 cut(s) 84, 202, 349, 415, 426, 716, 722
DpnII GATC 7 cut(s) 82, 200, 347, 413, 424, 714, 720
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
EciI GGCGGA 2 cut(s) 34, 52
Eco105I TACGTA 1 cut(s) 939
Eco31I GGTCTC 2 cut(s) 201, 499
Eco32I GATATC 1 cut(s) 744
Eco47I GGWCC 2 cut(s) 391, 419
EcoO109I RGGNCCY 2 cut(s) 391, 419
EcoRV GATATC 1 cut(s) 744
Esp3I CGTCTC 1 cut(s) 876
FaeI CATG 4 cut(s) 195, 243, 580, 750
FaiI YATR 8 cut(s) 123, 193, 241, 388, 578, 748, 942, 1050
FalI AAGNNNNNCTT 4 cut(s) 459, 491, 576, 608
FaqI GGGAC 1 cut(s) 404
FatI CATG 4 cut(s) 191, 239, 576, 746
FauI CCCGC 1 cut(s) 814
FblI GTMKAC 1 cut(s) 654
FokI GGATG 4 cut(s) 42, 88, 764, 976
FspBI CTAG 4 cut(s) 272, 636, 960, 987
GsuI CTGGAG 1 cut(s) 355
HapII CCGG 9 cut(s) 135, 307, 371, 428, 435, 462, 643, 794, 823
HgaI GACGC 2 cut(s) 149, 222
Hin1II CATG 4 cut(s) 195, 243, 580, 750
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HinfI GANTC 5 cut(s) 397, 431, 541, 580, 802
HpaI GTTAAC 1 cut(s) 625
HpaII CCGG 9 cut(s) 135, 307, 371, 428, 435, 462, 643, 794, 823
Hpy166II GTNNAC 3 cut(s) 562, 625, 655
Hpy188I TCNGA 2 cut(s) 212, 701
Hpy188III TCNNGA 8 cut(s) 198, 272, 334, 363, 417, 428, 577, 718
Hpy8I GTNNAC 3 cut(s) 562, 625, 655
Hpy99I CGWCG 8 cut(s) 83, 94, 143, 216, 614, 632, 857, 925
HpyAV CCTTC 7 cut(s) 69, 214, 236, 307, 313, 839, 895
HpyCH4III ACNGT 3 cut(s) 90, 284, 921
HpyCH4IV ACGT 5 cut(s) 171, 444, 612, 855, 938
HpyCH4V TGCA 1 cut(s) 243
HpyF3I CTNAG 2 cut(s) 593, 1023
HpySE526I ACGT 5 cut(s) 171, 444, 612, 855, 938
Hsp92II CATG 4 cut(s) 195, 243, 580, 750
Kpn2I TCCGGA 1 cut(s) 427
KspAI GTTAAC 1 cut(s) 625
Kzo9I GATC 7 cut(s) 82, 200, 347, 413, 424, 714, 720
LguI GCTCTTC 1 cut(s) 102
LmnI GCTCC 2 cut(s) 35, 1011
LweI GCATC 1 cut(s) 622
MaeI CTAG 4 cut(s) 272, 636, 960, 987
MaeII ACGT 5 cut(s) 171, 444, 612, 855, 938
MaeIII GTNAC 3 cut(s) 278, 284, 730
MalI GATC 7 cut(s) 84, 202, 349, 415, 426, 716, 722
MboI GATC 7 cut(s) 82, 200, 347, 413, 424, 714, 720
MboII GAAGA 7 cut(s) 89, 467, 945, 948, 960, 1010, 1013
MluCI AATT 4 cut(s) 253, 407, 695, 907
MlyI GAGTC 1 cut(s) 391
MmeI TCCRAC 1 cut(s) 235
MnlI CCTC 9 cut(s) 43, 142, 218, 482, 504, 695, 832, 1002, 1050
MroI TCCGGA 1 cut(s) 427
MseI TTAA 3 cut(s) 615, 624, 789
MspI CCGG 9 cut(s) 135, 307, 371, 428, 435, 462, 643, 794, 823
MspR9I CCNGG 1 cut(s) 436
MvnI CGCG 1 cut(s) 24
NciI CCSGG 1 cut(s) 436
NdeII GATC 7 cut(s) 82, 200, 347, 413, 424, 714, 720
NlaIII CATG 4 cut(s) 195, 243, 580, 750
NlaIV GGNNCC 3 cut(s) 31, 392, 421
NmuCI GTSAC 2 cut(s) 284, 730
PagI TCATGA 1 cut(s) 576
PciSI GCTCTTC 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 929
PfeI GAWTC 4 cut(s) 431, 541, 580, 802
PflFI GACNNNGTC 1 cut(s) 887
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
Ppu21I YACGTR 2 cut(s) 445, 939
PpuMI RGGWCCY 2 cut(s) 391, 419
Psp1406I AACGTT 1 cut(s) 171
Psp5II RGGWCCY 2 cut(s) 391, 419
PspN4I GGNNCC 3 cut(s) 31, 392, 421
PspPI GGNCC 2 cut(s) 391, 419
PspPPI RGGWCCY 2 cut(s) 391, 419
PsyI GACNNNGTC 1 cut(s) 887
RsaI GTAC 2 cut(s) 161, 937
RsaNI GTAC 2 cut(s) 160, 936
SapI GCTCTTC 1 cut(s) 102
SaqAI TTAA 3 cut(s) 615, 624, 789
Sau3AI GATC 7 cut(s) 82, 200, 347, 413, 424, 714, 720
Sau96I GGNCC 2 cut(s) 391, 419
SchI GAGTC 1 cut(s) 391
ScrFI CCNGG 1 cut(s) 436
SfaNI GCATC 1 cut(s) 622
SinI GGWCC 2 cut(s) 391, 419
SnaBI TACGTA 1 cut(s) 939
Sse9I AATT 4 cut(s) 253, 407, 695, 907
SsiI CCGC 5 cut(s) 24, 45, 63, 69, 807
SspMI CTAG 4 cut(s) 272, 636, 960, 987
StyD4I CCNGG 1 cut(s) 434
TaaI ACNGT 3 cut(s) 90, 284, 921
TaiI ACGT 5 cut(s) 174, 447, 615, 858, 941
TaqI TCGA 6 cut(s) 199, 312, 364, 609, 723, 913
TaqII GACCGA 1 cut(s) 523
TasI AATT 4 cut(s) 253, 407, 695, 907
TfiI GAWTC 4 cut(s) 431, 541, 580, 802
Tru1I TTAA 3 cut(s) 615, 624, 789
Tru9I TTAA 3 cut(s) 615, 624, 789
TseFI GTSAC 2 cut(s) 284, 730
Tsp45I GTSAC 2 cut(s) 284, 730
TspDTI ATGAA 4 cut(s) 17, 180, 218, 881
TspGWI ACGGA 1 cut(s) 913
Tth111I GACNNNGTC 1 cut(s) 887
VpaK11BI GGWCC 2 cut(s) 391, 419
XapI RAATTY 2 cut(s) 407, 695
XbaI TCTAGA 1 cut(s) 271
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XmiI GTMKAC 1 cut(s) 654
XspI CTAG 4 cut(s) 272, 636, 960, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.