MD15G1036800.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013041
Physical Location & Seq
Forward (+)
2568281 .. 2569315
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1036800.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGTCCCGCCACTACTCCTCCGCTTTCCCTTCTTGCTTCCGCCCCTCCACCTCCATCAACCGTCGCCTCCCACCACCGTCTCCGCCGCCTCCCATCTCCGGGCACCCCAACCTCACCACCTGCCTCTACCACACCGACCACGCCCTCTTCTCCCTCACCTGGTCCCGCTCCTTCCTCGGCCGCTCCCTCCACCTCAACCTCCTCCACCACTCCTTCGACTCGCCCCTCCCTCCGTCGCTCTCCTCCCCTTACTTCCACCTCCACATCAAGCCCTTTCTCTTCTGGAAGAAGCACGGCTCCAAGAAGCTCTCCCCCAACATTCTCCTCTTCTGGGACCTCTCCAAAGCCCGGTTCAGCTCCGGACCTGAACCCCAATCCGGGTTCTACATCGCCGTCGTCGTCGACAACGAGATGACCCTCCTCGTCGGCGATTTGACCCGCGAAGCCTACTCCAAGACCCGGGCCCGAAAATCTGAGACTCCGCAAATCCCAATCTTGAAAAGAGAGCACGTCATCGCCAAGAAAATCTACACCACTATGGCCCGGTTCGGCGGTAAATTGCGGGAGATCCGAATCGATTGTGGGTCAAACGCCGACCCGACCCGGCTCTGCTTTAGCGTCGATGGCCAGGTTGTCCTGCAGATAAAGCGGTTGAAGTGGAAATTCAGAGGGAACGAGCGAATCGAAGTCGACGGGACCCCGCTGCAGATCTCGTGGGACGTCTACAACTGGCTTTTCGAGAGCGACGGTTGCCGCGACGACGGCCACGCGGTGTTCATGTTCAGATTCGAAGAAGACGAGGAGATTAAAGAGAACAATTACCAACTGGGTCATCTGAATTCGTGGAATTTCGGGATGAGCGGGATTGAGTGGCGGAAGATGGGGAAGAGCTTGTCTTCTTCGTCGGTTTCGATGTCGTCGGCGGGGTCTTCCGGTGGAAGTTCGTCGGTGATGGAGTGGGCGAGCACGGAGGAGAGCGAGCTCAGCGGCGGTCCTGCTGGGTTTTCTTTGCTGGTTTATGCCTGGAAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

345

Amino Acids

38.95

Weight (kDa)

9.21

Isoelectric Point (pI)

60.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 36 - 343 5.4e-78 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013041)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25200 AT5G11000
fragaria_vesca FvH4_2g40820
malus_domestica MD08G1059500.v1.1 MD15G1036800.v1.1
prunus_persica Prupe.1G390900_v2.0.a1
pyrus_communis pycom08g04960 pycom15g03410
rosa_chinensis RchiOBHm_Chr6g0299751
rosa_laevigata RLG00000011391
rosa_multiflora Rmu_sc0002895.1_g000019
rosa_roxburghii Rroxscaffold_2G00123250
rosa_rugosa Rorug06G0292100
rosa_samantha Rh6AG402900 Rh6BG411500 Rh6CG417600 Rh6DG404400
rosa_wichuraiana Rw6G035270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 128
AatII GACGTC 1 cut(s) 723
Acc36I ACCTGC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 102
AccBSI CCGCTC 3 cut(s) 168, 183, 861
AccI GTMKAC 3 cut(s) 402, 690, 723
AccII CGCG 3 cut(s) 441, 756, 770
AccIII TCCGGA 1 cut(s) 359
AclWI GGATC 1 cut(s) 562
AcoI YGGCCR 3 cut(s) 178, 625, 763
AcsI RAATTY 3 cut(s) 662, 838, 847
AcyI GRCGYC 1 cut(s) 720
AdeI CACNNNGTG 1 cut(s) 772
AfiI CCNNNNNNNGG 9 cut(s) 98, 99, 159, 331, 348, 377, 378, 459, 1029
AgsI TTSAA 2 cut(s) 499, 655
AjiI CACGTC 1 cut(s) 511
AjnI CCWGG 3 cut(s) 158, 627, 1022
AjuI GAANNNNNNNTTGG 2 cut(s) 335, 367
AluBI AGCT 4 cut(s) 307, 357, 891, 982
AluI AGCT 4 cut(s) 307, 357, 891, 982
Alw21I GWGCWC 3 cut(s) 510, 968, 984
Alw26I GTCTC 2 cut(s) 84, 470
AlwI GGATC 1 cut(s) 562
Ama87I CYCGRG 1 cut(s) 459
Aor13HI TCCGGA 1 cut(s) 359
AoxI GGCC 5 cut(s) 178, 462, 540, 625, 763
ApaI GGGCCC 1 cut(s) 466
ApeKI GCWGC 1 cut(s) 703
ApoI RAATTY 3 cut(s) 662, 838, 847
AspS9I GGNCC 8 cut(s) 162, 334, 362, 462, 463, 541, 696, 992
AsuC2I CCSGG 7 cut(s) 100, 349, 379, 460, 461, 544, 604
AsuHPI GGTGA 3 cut(s) 106, 148, 961
AsuII TTCGAA 1 cut(s) 789
AvaI CYCGRG 1 cut(s) 459
AvaII GGWCC 5 cut(s) 162, 334, 362, 696, 992
BaeGI GKGCMC 2 cut(s) 105, 466
BalI TGGCCA 1 cut(s) 627
BanI GGYRCC 1 cut(s) 102
BanII GRGCYC 2 cut(s) 466, 984
BauI CACGAG 1 cut(s) 712
BbsI GAAGAC 3 cut(s) 801, 888, 921
Bbv12I GWGCWC 3 cut(s) 510, 968, 984
BbvI GCAGC 1 cut(s) 690
BccI CCATC 5 cut(s) 62, 101, 617, 874, 946
BceAI ACGGC 3 cut(s) 310, 377, 778
BciT130I CCWGG 3 cut(s) 160, 629, 1024
BcnI CCSGG 7 cut(s) 100, 349, 379, 460, 461, 544, 604
BcoDI GTCTC 2 cut(s) 84, 470
BfmI CTRYAG 2 cut(s) 638, 704
BfuAI ACCTGC 1 cut(s) 128
BglII AGATCT 1 cut(s) 708
BisI GCNGC 5 cut(s) 86, 181, 704, 754, 988
BlpI GCTNAGC 1 cut(s) 983
BlsI GCNGC 5 cut(s) 87, 182, 705, 755, 989
Bme18I GGWCC 5 cut(s) 162, 334, 362, 696, 992
BmeT110I CYCGRG 1 cut(s) 459
BmgBI CACGTC 1 cut(s) 511
BmgT120I GGNCC 8 cut(s) 162, 334, 362, 462, 463, 541, 696, 992
BmiI GGNNCC 7 cut(s) 104, 164, 298, 335, 464, 697, 698
BmrI ACTGGG 1 cut(s) 836
BmuI ACTGGG 1 cut(s) 836
BpiI GAAGAC 3 cut(s) 801, 888, 921
Bpu1102I GCTNAGC 1 cut(s) 983
Bpu14I TTCGAA 1 cut(s) 789
BpuMI CCSGG 7 cut(s) 100, 349, 379, 460, 461, 544, 604
Bsa29I ATCGAT 1 cut(s) 576
BsaBI GATNNNNATC 1 cut(s) 572
BsaHI GRCGYC 1 cut(s) 720
BsaJI CCNNGG 2 cut(s) 175, 459
BsaWI WCCGGW 2 cut(s) 359, 932
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 9 cut(s) 98, 99, 159, 331, 348, 377, 378, 459, 1029
Bse1I ACTGG 2 cut(s) 734, 831
Bse8I GATNNNNATC 1 cut(s) 572
BseAI TCCGGA 1 cut(s) 359
BseBI CCWGG 3 cut(s) 160, 629, 1024
BseCI ATCGAT 1 cut(s) 576
BseDI CCNNGG 2 cut(s) 175, 459
BseGI GGATG 1 cut(s) 861
BseJI GATNNNNATC 1 cut(s) 572
BseLI CCNNNNNNNGG 9 cut(s) 98, 99, 159, 331, 348, 377, 378, 459, 1029
BseMII CTCAG 2 cut(s) 465, 997
BseNI ACTGG 2 cut(s) 734, 831
BseRI GAGGAG 7 cut(s) 7, 191, 232, 314, 410, 815, 986
BseSI GKGCMC 2 cut(s) 105, 466
BseX3I CGGCCG 1 cut(s) 178
BseXI GCAGC 1 cut(s) 690
BseYI CCCAGC 1 cut(s) 998
Bsh1236I CGCG 3 cut(s) 441, 756, 770
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 5 cut(s) 180, 464, 542, 627, 765
BshNI GGYRCC 1 cut(s) 102
BshVI ATCGAT 1 cut(s) 576
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 3 cut(s) 510, 968, 984
BsiHKCI CYCGRG 1 cut(s) 459
BsiSI CCGG 8 cut(s) 99, 349, 360, 378, 460, 544, 604, 933
BslFI GGGAC 4 cut(s) 148, 347, 709, 731
BslI CCNNNNNNNGG 9 cut(s) 98, 99, 159, 331, 348, 377, 378, 459, 1029
BsmAI GTCTC 2 cut(s) 84, 470
BsmBI CGTCTC 1 cut(s) 84
BsmFI GGGAC 4 cut(s) 148, 347, 709, 731
BsnI GGCC 5 cut(s) 180, 464, 542, 627, 765
BsoBI CYCGRG 1 cut(s) 459
Bsp119I TTCGAA 1 cut(s) 789
Bsp120I GGGCCC 1 cut(s) 462
Bsp1286I GDGCHC 5 cut(s) 105, 466, 510, 968, 984
Bsp13I TCCGGA 1 cut(s) 359
Bsp143I GATC 2 cut(s) 567, 708
Bsp1720I GCTNAGC 1 cut(s) 983
BspANI GGCC 5 cut(s) 180, 464, 542, 627, 765
BspCNI CTCAG 2 cut(s) 466, 996
BspDI ATCGAT 1 cut(s) 576
BspEI TCCGGA 1 cut(s) 359
BspFNI CGCG 3 cut(s) 441, 756, 770
BspLI GGNNCC 7 cut(s) 104, 164, 298, 335, 464, 697, 698
BspMAI CTGCAG 2 cut(s) 642, 708
BspMI ACCTGC 1 cut(s) 128
BspPI GGATC 1 cut(s) 562
BspQI GCTCTTC 1 cut(s) 881
BspT104I TTCGAA 1 cut(s) 789
BspT107I GGYRCC 1 cut(s) 102
BsrBI CCGCTC 3 cut(s) 168, 183, 861
BsrI ACTGG 2 cut(s) 734, 831
BssECI CCNNGG 2 cut(s) 175, 459
BssMI GATC 2 cut(s) 567, 708
BssNI GRCGYC 1 cut(s) 720
BssSI CACGAG 1 cut(s) 712
Bst2BI CACGAG 1 cut(s) 712
Bst2UI CCWGG 3 cut(s) 160, 629, 1024
Bst4CI ACNGT 3 cut(s) 62, 78, 749
Bst6I CTCTTC 4 cut(s) 152, 284, 332, 881
BstACI GRCGYC 1 cut(s) 720
BstBI TTCGAA 1 cut(s) 789
BstC8I GCNNGC 2 cut(s) 964, 980
BstDEI CTNAG 2 cut(s) 474, 983
BstENI CCTNNNNNAGG 1 cut(s) 1027
BstF5I GGATG 1 cut(s) 861
BstFNI CGCG 3 cut(s) 441, 756, 770
BstKTI GATC 2 cut(s) 570, 711
BstMAI GTCTC 2 cut(s) 84, 470
BstMBI GATC 2 cut(s) 567, 708
BstMCI CGRYCG 1 cut(s) 181
BstMWI GCNNNNNNNGC 5 cut(s) 624, 646, 750, 762, 984
BstNI CCWGG 3 cut(s) 160, 629, 1024
BstSFI CTRYAG 2 cut(s) 638, 704
BstSLI GKGCMC 2 cut(s) 105, 466
BstUI CGCG 3 cut(s) 441, 756, 770
BstV1I GCAGC 1 cut(s) 690
BstV2I GAAGAC 3 cut(s) 801, 888, 921
BstX2I RGATCY 2 cut(s) 567, 708
BstYI RGATCY 2 cut(s) 567, 708
BstZI CGGCCG 1 cut(s) 178
Bsu15I ATCGAT 1 cut(s) 576
BsuRI GGCC 5 cut(s) 180, 464, 542, 627, 765
BsuTUI ATCGAT 1 cut(s) 576
BtgZI GCGATG 2 cut(s) 373, 499
BtrI CACGTC 1 cut(s) 511
BtsCI GGATG 1 cut(s) 861
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 2 cut(s) 964, 980
Cfr13I GGNCC 8 cut(s) 162, 334, 362, 462, 463, 541, 696, 992
Cfr9I CCCGGG 1 cut(s) 459
ClaI ATCGAT 1 cut(s) 576
CseI GACGC 1 cut(s) 607
CsiI ACCWGGT 1 cut(s) 158
CviAII CATG 1 cut(s) 778
DdeI CTNAG 2 cut(s) 474, 983
DpnI GATC 2 cut(s) 569, 710
DpnII GATC 2 cut(s) 567, 708
DraIII CACNNNGTG 1 cut(s) 772
EaeI YGGCCR 3 cut(s) 178, 625, 763
EagI CGGCCG 1 cut(s) 178
Eam1104I CTCTTC 4 cut(s) 152, 284, 332, 881
EarI CTCTTC 4 cut(s) 152, 284, 332, 881
EciI GGCGGA 3 cut(s) 29, 72, 889
Ecl136II GAGCTC 1 cut(s) 982
EclXI CGGCCG 1 cut(s) 178
Eco24I GRGCYC 2 cut(s) 466, 984
Eco47I GGWCC 5 cut(s) 162, 334, 362, 696, 992
Eco52I CGGCCG 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 982
Eco88I CYCGRG 1 cut(s) 459
EcoICRI GAGCTC 1 cut(s) 982
EcoNI CCTNNNNNAGG 1 cut(s) 1027
EcoO109I RGGNCCY 2 cut(s) 334, 696
EcoRI GAATTC 1 cut(s) 838
EcoRII CCWGG 3 cut(s) 158, 627, 1022
EcoT38I GRGCYC 2 cut(s) 466, 984
Esp3I CGTCTC 1 cut(s) 84
FaeI CATG 1 cut(s) 781
FaiI YATR 3 cut(s) 539, 779, 1020
FaqI GGGAC 4 cut(s) 148, 347, 709, 731
FatI CATG 1 cut(s) 777
FauI CCCGC 7 cut(s) 14, 173, 446, 555, 708, 854, 916
FblI GTMKAC 3 cut(s) 402, 690, 723
Fnu4HI GCNGC 5 cut(s) 86, 181, 704, 754, 988
FokI GGATG 1 cut(s) 868
FriOI GRGCYC 2 cut(s) 466, 984
Fsp4HI GCNGC 5 cut(s) 86, 181, 704, 754, 988
GluI GCNGC 5 cut(s) 86, 181, 704, 754, 988
GsaI CCCAGC 1 cut(s) 1002
HaeIII GGCC 5 cut(s) 180, 464, 542, 627, 765
HapII CCGG 8 cut(s) 99, 349, 360, 378, 460, 544, 604, 933
HgaI GACGC 1 cut(s) 607
Hin1I GRCGYC 1 cut(s) 720
Hin1II CATG 1 cut(s) 781
HincII GTYRAC 2 cut(s) 403, 691
HindII GTYRAC 2 cut(s) 403, 691
HinfI GANTC 5 cut(s) 218, 478, 573, 681, 786
HpaII CCGG 8 cut(s) 99, 349, 360, 378, 460, 544, 604, 933
HphI GGTGA 3 cut(s) 106, 148, 961
Hpy166II GTNNAC 3 cut(s) 403, 691, 724
Hpy188I TCNGA 5 cut(s) 475, 572, 668, 785, 837
Hpy188III TCNNGA 5 cut(s) 283, 360, 496, 739, 853
Hpy8I GTNNAC 3 cut(s) 403, 691, 724
HpyAV CCTTC 3 cut(s) 39, 181, 223
HpyCH4III ACNGT 3 cut(s) 62, 78, 749
HpyCH4IV ACGT 2 cut(s) 510, 720
HpyCH4V TGCA 2 cut(s) 640, 706
HpyF10VI GCNNNNNNNGC 5 cut(s) 624, 646, 750, 762, 984
HpyF3I CTNAG 2 cut(s) 474, 983
HpySE526I ACGT 2 cut(s) 510, 720
Hsp92I GRCGYC 1 cut(s) 720
Hsp92II CATG 1 cut(s) 781
KflI GGGWCCC 1 cut(s) 696
Kpn2I TCCGGA 1 cut(s) 359
Kzo9I GATC 2 cut(s) 567, 708
LguI GCTCTTC 1 cut(s) 881
LmnI GCTCC 4 cut(s) 173, 188, 302, 362
Lsp1109I GCAGC 1 cut(s) 690
MabI ACCWGGT 1 cut(s) 158
MaeII ACGT 2 cut(s) 510, 720
MalI GATC 2 cut(s) 569, 710
MbiI CCGCTC 3 cut(s) 168, 183, 861
MboI GATC 2 cut(s) 567, 708
MflI RGATCY 2 cut(s) 567, 708
MhlI GDGCHC 5 cut(s) 105, 466, 510, 968, 984
MlsI TGGCCA 1 cut(s) 627
MluCI AATT 5 cut(s) 557, 662, 817, 838, 847
MluNI TGGCCA 1 cut(s) 627
MlyI GAGTC 2 cut(s) 212, 472
Mox20I TGGCCA 1 cut(s) 627
MroI TCCGGA 1 cut(s) 359
MscI TGGCCA 1 cut(s) 627
MseI TTAA 1 cut(s) 807
MslI CAYNNNNRTG 1 cut(s) 536
Msp20I TGGCCA 1 cut(s) 627
MspA1I CMGCKG 2 cut(s) 703, 987
MspI CCGG 8 cut(s) 99, 349, 360, 378, 460, 544, 604, 933
MvaI CCWGG 3 cut(s) 160, 629, 1024
MvnI CGCG 3 cut(s) 441, 756, 770
MwoI GCNNNNNNNGC 5 cut(s) 624, 646, 750, 762, 984
NciI CCSGG 7 cut(s) 100, 349, 379, 460, 461, 544, 604
NdeII GATC 2 cut(s) 567, 708
NlaIII CATG 1 cut(s) 781
NlaIV GGNNCC 7 cut(s) 104, 164, 298, 335, 464, 697, 698
NmeAIII GCCGAG 1 cut(s) 156
NspV TTCGAA 1 cut(s) 789
PaqCI CACCTGC 1 cut(s) 128
PciSI GCTCTTC 1 cut(s) 881
PcsI WCGNNNNNNNCGW 6 cut(s) 396, 405, 681, 753, 795, 908
PfeI GAWTC 3 cut(s) 573, 681, 786
PkrI GCNGC 5 cut(s) 87, 182, 705, 755, 989
PleI GAGTC 2 cut(s) 212, 472
PpsI GAGTC 2 cut(s) 212, 472
PpuMI RGGWCCY 2 cut(s) 334, 696
Psp124BI GAGCTC 1 cut(s) 984
Psp5II RGGWCCY 2 cut(s) 334, 696
Psp6I CCWGG 3 cut(s) 158, 627, 1022
PspFI CCCAGC 1 cut(s) 998
PspGI CCWGG 3 cut(s) 158, 627, 1022
PspN4I GGNNCC 7 cut(s) 104, 164, 298, 335, 464, 697, 698
PspOMI GGGCCC 1 cut(s) 462
PspPI GGNCC 8 cut(s) 162, 334, 362, 462, 463, 541, 696, 992
PspPPI RGGWCCY 2 cut(s) 334, 696
PstI CTGCAG 2 cut(s) 642, 708
PsuI RGATCY 2 cut(s) 567, 708
RseI CAYNNNNRTG 1 cut(s) 536
SacI GAGCTC 1 cut(s) 984
SalI GTCGAC 2 cut(s) 401, 689
SapI GCTCTTC 1 cut(s) 881
SaqAI TTAA 1 cut(s) 807
SatI GCNGC 5 cut(s) 86, 181, 704, 754, 988
Sau3AI GATC 2 cut(s) 567, 708
Sau96I GGNCC 8 cut(s) 162, 334, 362, 462, 463, 541, 696, 992
SchI GAGTC 2 cut(s) 212, 472
SduI GDGCHC 5 cut(s) 105, 466, 510, 968, 984
SexAI ACCWGGT 1 cut(s) 158
SfcI CTRYAG 2 cut(s) 638, 704
SfuI TTCGAA 1 cut(s) 789
SinI GGWCC 5 cut(s) 162, 334, 362, 696, 992
SmaI CCCGGG 1 cut(s) 461
SmiMI CAYNNNNRTG 1 cut(s) 536
Sse9I AATT 5 cut(s) 557, 662, 817, 838, 847
SstI GAGCTC 1 cut(s) 984
TaaI ACNGT 3 cut(s) 62, 78, 749
TaiI ACGT 2 cut(s) 513, 723
TaqI TCGA 9 cut(s) 216, 402, 576, 621, 684, 690, 738, 789, 911
TasI AATT 5 cut(s) 557, 662, 817, 838, 847
TauI GCSGC 4 cut(s) 88, 183, 756, 990
TfiI GAWTC 3 cut(s) 573, 681, 786
Tru1I TTAA 1 cut(s) 807
Tru9I TTAA 1 cut(s) 807
TseI GCWGC 1 cut(s) 703
TspDTI ATGAA 1 cut(s) 766
TspGWI ACGGA 2 cut(s) 222, 983
TspMI CCCGGG 1 cut(s) 459
VpaK11BI GGWCC 5 cut(s) 162, 334, 362, 696, 992
XagI CCTNNNNNAGG 1 cut(s) 1027
XapI RAATTY 3 cut(s) 662, 838, 847
XmaI CCCGGG 1 cut(s) 459
XmiI GTMKAC 3 cut(s) 402, 690, 723
ZraI GACGTC 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.