MD08G1059500.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013041
Physical Location & Seq
Reverse (-)
4756239 .. 4757279
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1059500.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGTCCCGCCACAACTCCTCCTCCGCTTTTCCGTCTTGCTTCCGCCCCTCCACCTCCACCAACCGCCGCCTCCCACCACCGTCTCCGCTTCCTCCTCCGCCGCCCAGCTCCGGGCAACCCAACCTCACCACGTGCCTCTACCACACAGACCTCGCCCTCTTCTCCCTCACCTGGTCCCGCTCCTTCCTCGGCCGCTCCCTCCACCTCAACCTTCTCCGCCACTCCTTCGATTCCCCCCTCCCTCCGTCGCTATCCTCCCCTTCTTTCCACCTCCACATCAAGCCCTTCCTCTTCTGGAAGAAGCACGGCTCCAAGAAGCTCTCCCCCAACATTCTCCTCTACTGGGACCTCTCCAGAGCCCGGTTCGGCTCCGGACCCGAACCCCAATCCGGGTTCTACATCGCCGTCGTCGTCGACAACGAGATGACCCTCCTCGTCGGCGATTTGACCCGCGAAGCCTACGCCAAGACCCGGGCCAACAAATCAGAGACCTCTCAAATCCCAATCTTGAAACGAGAGCACGTCGTTGCCAAGAAAATCTACACCACGAAAGCCCGGTTCGGCGGAAAATTGCGGGAGATCCGAATCGATTACGGGTCGAGCGCCGACCCGACCCGGCTATGCTTCAGCGTCGACGGCCAAATTGTCCTGCAGATAAAGCGGTTGAAGTGGAAATTCAGAGGGAACGAGCGAATCGAAGTCGACGGGACTCCGGTGCAGATCTCGTGGGACGTCTACAACTGGCTTTTCGAGAGCGACGGCGACGACGGCCACGCGGTGTTCATGTTCAGATTCGAAGAAGGCGAGGAGATTAAAGAGAACAATTACCAATTCGGTCATCTGAATTCGTGGAATTTCGGGATGAGCGGGATTGAGTGGCGAAAGATGGGGAAGAGCTTCTCTTCTTCGTCGGTATCGATGTCGTCGGCGGGTTCTTCCGGCGGAAGCTCGTCGGTGATGGAGTGGGCGAGTACGGAGGAGAGCGAGCTCAACGGCGGTCCTGCTGGTTTTTCTCTGCAGATTTATGCTTGGAAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

347

Amino Acids

38.94

Weight (kDa)

9.42

Isoelectric Point (pI)

64.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 40 - 345 5.3e-80 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013041)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25200 AT5G11000
fragaria_vesca FvH4_2g40820
malus_domestica MD08G1059500.v1.1 MD15G1036800.v1.1
prunus_persica Prupe.1G390900_v2.0.a1
pyrus_communis pycom08g04960 pycom15g03410
rosa_chinensis RchiOBHm_Chr6g0299751
rosa_laevigata RLG00000011391
rosa_multiflora Rmu_sc0002895.1_g000019
rosa_roxburghii Rroxscaffold_2G00123250
rosa_rugosa Rorug06G0292100
rosa_samantha Rh6AG402900 Rh6BG411500 Rh6CG417600 Rh6DG404400
rosa_wichuraiana Rw6G035270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 735
AccBSI CCGCTC 3 cut(s) 180, 195, 867
AccI GTMKAC 4 cut(s) 414, 633, 702, 735
AccII CGCG 2 cut(s) 453, 776
AccIII TCCGGA 1 cut(s) 371
AclWI GGATC 1 cut(s) 574
AcoI YGGCCR 3 cut(s) 190, 637, 769
AcsI RAATTY 3 cut(s) 674, 844, 853
AcuI CTGAAG 1 cut(s) 610
AcvI CACGTG 1 cut(s) 132
AcyI GRCGYC 1 cut(s) 732
AdeI CACNNNGTG 2 cut(s) 132, 778
AfaI GTAC 1 cut(s) 973
AfiI CCNNNNNNNGG 8 cut(s) 110, 111, 171, 343, 360, 389, 390, 471
AgsI TTSAA 2 cut(s) 511, 667
AjiI CACGTC 1 cut(s) 523
AjnI CCWGG 1 cut(s) 170
AluBI AGCT 5 cut(s) 108, 319, 897, 948, 988
AluI AGCT 5 cut(s) 108, 319, 897, 948, 988
Alw21I GWGCWC 2 cut(s) 522, 990
Alw26I GTCTC 2 cut(s) 87, 482
AlwI GGATC 1 cut(s) 574
Ama87I CYCGRG 1 cut(s) 471
Aor13HI TCCGGA 1 cut(s) 371
AoxI GGCC 4 cut(s) 190, 474, 637, 769
ApoI RAATTY 3 cut(s) 674, 844, 853
Asp700I GAANNNNTTC 1 cut(s) 896
AspLEI GCGC 1 cut(s) 605
AspS9I GGNCC 5 cut(s) 174, 346, 374, 474, 998
AsuC2I CCSGG 7 cut(s) 112, 361, 391, 472, 473, 556, 616
AsuHPI GGTGA 3 cut(s) 118, 160, 967
AsuII TTCGAA 1 cut(s) 795
AvaI CYCGRG 1 cut(s) 471
AvaII GGWCC 4 cut(s) 174, 346, 374, 998
BanII GRGCYC 2 cut(s) 361, 990
BauI CACGAG 1 cut(s) 724
BbrPI CACGTG 1 cut(s) 132
Bbv12I GWGCWC 2 cut(s) 522, 990
BccI CCATC 2 cut(s) 880, 952
BceAI ACGGC 6 cut(s) 322, 389, 652, 775, 784, 1009
BciT130I CCWGG 1 cut(s) 172
BcnI CCSGG 7 cut(s) 112, 361, 391, 472, 473, 556, 616
BcoDI GTCTC 2 cut(s) 87, 482
BfmI CTRYAG 2 cut(s) 650, 1016
BfoI RGCGCY 1 cut(s) 606
BglII AGATCT 1 cut(s) 720
BisI GCNGC 3 cut(s) 67, 101, 193
BlsI GCNGC 3 cut(s) 68, 102, 194
Bme1390I CCNGG 8 cut(s) 112, 172, 361, 391, 472, 473, 556, 616
Bme18I GGWCC 4 cut(s) 174, 346, 374, 998
BmeT110I CYCGRG 1 cut(s) 471
BmgBI CACGTC 1 cut(s) 523
BmgT120I GGNCC 5 cut(s) 174, 346, 374, 474, 998
BmiI GGNNCC 5 cut(s) 176, 310, 347, 370, 376
BmrFI CCNGG 8 cut(s) 112, 172, 361, 391, 472, 473, 556, 616
BmrI ACTGGG 1 cut(s) 352
BmuI ACTGGG 1 cut(s) 352
BpmI CTGGAG 1 cut(s) 337
Bpu14I TTCGAA 1 cut(s) 795
BpuMI CCSGG 7 cut(s) 112, 361, 391, 472, 473, 556, 616
Bsa29I ATCGAT 2 cut(s) 588, 917
BsaAI YACGTR 1 cut(s) 132
BsaBI GATNNNNATC 1 cut(s) 584
BsaHI GRCGYC 1 cut(s) 732
BsaI GGTCTC 1 cut(s) 482
BsaJI CCNNGG 2 cut(s) 187, 471
BsaWI WCCGGW 2 cut(s) 371, 712
BsaXI ACNNNNNCTCC 3 cut(s) 5, 32, 35
Bsc4I CCNNNNNNNGG 8 cut(s) 110, 111, 171, 343, 360, 389, 390, 471
Bse1I ACTGG 2 cut(s) 347, 746
Bse8I GATNNNNATC 1 cut(s) 584
BseAI TCCGGA 1 cut(s) 371
BseBI CCWGG 1 cut(s) 172
BseCI ATCGAT 2 cut(s) 588, 917
BseDI CCNNGG 2 cut(s) 187, 471
BseGI GGATG 1 cut(s) 867
BseJI GATNNNNATC 1 cut(s) 584
BseLI CCNNNNNNNGG 8 cut(s) 110, 111, 171, 343, 360, 389, 390, 471
BseNI ACTGG 2 cut(s) 347, 746
BseRI GAGGAG 7 cut(s) 7, 10, 84, 326, 422, 821, 992
BseX3I CGGCCG 1 cut(s) 190
BseYI CCCAGC 1 cut(s) 104
BsgI GTGCAG 1 cut(s) 737
Bsh1236I CGCG 2 cut(s) 453, 776
Bsh1285I CGRYCG 1 cut(s) 193
BshFI GGCC 4 cut(s) 192, 476, 639, 771
BshVI ATCGAT 2 cut(s) 588, 917
BsiEI CGRYCG 1 cut(s) 193
BsiHKAI GWGCWC 2 cut(s) 522, 990
BsiHKCI CYCGRG 1 cut(s) 471
BsiSI CCGG 9 cut(s) 111, 361, 372, 390, 472, 556, 616, 713, 939
BslFI GGGAC 4 cut(s) 160, 359, 721, 743
BslI CCNNNNNNNGG 8 cut(s) 110, 111, 171, 343, 360, 389, 390, 471
BsmAI GTCTC 2 cut(s) 87, 482
BsmBI CGTCTC 1 cut(s) 87
BsmFI GGGAC 4 cut(s) 160, 359, 721, 743
BsnI GGCC 4 cut(s) 192, 476, 639, 771
Bso31I GGTCTC 1 cut(s) 482
BsoBI CYCGRG 1 cut(s) 471
Bsp119I TTCGAA 1 cut(s) 795
Bsp1286I GDGCHC 3 cut(s) 361, 522, 990
Bsp13I TCCGGA 1 cut(s) 371
Bsp143I GATC 2 cut(s) 579, 720
BspANI GGCC 4 cut(s) 192, 476, 639, 771
BspDI ATCGAT 2 cut(s) 588, 917
BspEI TCCGGA 1 cut(s) 371
BspFNI CGCG 2 cut(s) 453, 776
BspLI GGNNCC 5 cut(s) 176, 310, 347, 370, 376
BspMAI CTGCAG 2 cut(s) 654, 1020
BspPI GGATC 1 cut(s) 574
BspQI GCTCTTC 1 cut(s) 887
BspT104I TTCGAA 1 cut(s) 795
BspTNI GGTCTC 1 cut(s) 482
BsrBI CCGCTC 3 cut(s) 180, 195, 867
BsrI ACTGG 2 cut(s) 347, 746
BssECI CCNNGG 2 cut(s) 187, 471
BssMI GATC 2 cut(s) 579, 720
BssNI GRCGYC 1 cut(s) 732
BssSI CACGAG 1 cut(s) 724
Bst2BI CACGAG 1 cut(s) 724
Bst2UI CCWGG 1 cut(s) 172
Bst4CI ACNGT 1 cut(s) 81
Bst6I CTCTTC 4 cut(s) 164, 296, 887, 907
BstACI GRCGYC 1 cut(s) 732
BstBAI YACGTR 1 cut(s) 132
BstBI TTCGAA 1 cut(s) 795
BstC8I GCNNGC 1 cut(s) 986
BstF5I GGATG 1 cut(s) 867
BstFNI CGCG 2 cut(s) 453, 776
BstH2I RGCGCY 1 cut(s) 606
BstHHI GCGC 1 cut(s) 605
BstKTI GATC 2 cut(s) 582, 723
BstMAI GTCTC 2 cut(s) 87, 482
BstMBI GATC 2 cut(s) 579, 720
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 3 cut(s) 636, 658, 768
BstNI CCWGG 1 cut(s) 172
BstSCI CCNGG 8 cut(s) 110, 170, 359, 389, 470, 471, 554, 614
BstSFI CTRYAG 2 cut(s) 650, 1016
BstUI CGCG 2 cut(s) 453, 776
BstX2I RGATCY 2 cut(s) 579, 720
BstYI RGATCY 2 cut(s) 579, 720
BstZI CGGCCG 1 cut(s) 190
Bsu15I ATCGAT 2 cut(s) 588, 917
BsuRI GGCC 4 cut(s) 192, 476, 639, 771
BsuTUI ATCGAT 2 cut(s) 588, 917
BtgZI GCGATG 1 cut(s) 385
BtrI CACGTC 1 cut(s) 523
BtsCI GGATG 1 cut(s) 867
Cac8I GCNNGC 1 cut(s) 986
CfoI GCGC 1 cut(s) 605
Cfr13I GGNCC 5 cut(s) 174, 346, 374, 474, 998
Cfr9I CCCGGG 1 cut(s) 471
ClaI ATCGAT 2 cut(s) 588, 917
CseI GACGC 1 cut(s) 619
CsiI ACCWGGT 1 cut(s) 170
Csp6I GTAC 1 cut(s) 972
CviAII CATG 1 cut(s) 784
CviQI GTAC 1 cut(s) 972
DpnI GATC 2 cut(s) 581, 722
DpnII GATC 2 cut(s) 579, 720
DraIII CACNNNGTG 2 cut(s) 132, 778
EaeI YGGCCR 3 cut(s) 190, 637, 769
EagI CGGCCG 1 cut(s) 190
Eam1104I CTCTTC 4 cut(s) 164, 296, 887, 907
EarI CTCTTC 4 cut(s) 164, 296, 887, 907
EciI GGCGGA 5 cut(s) 32, 87, 206, 579, 957
Ecl136II GAGCTC 1 cut(s) 988
EclXI CGGCCG 1 cut(s) 190
Eco24I GRGCYC 2 cut(s) 361, 990
Eco31I GGTCTC 1 cut(s) 482
Eco47I GGWCC 4 cut(s) 174, 346, 374, 998
Eco52I CGGCCG 1 cut(s) 190
Eco53kI GAGCTC 1 cut(s) 988
Eco57I CTGAAG 1 cut(s) 610
Eco72I CACGTG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 471
EcoICRI GAGCTC 1 cut(s) 988
EcoO109I RGGNCCY 1 cut(s) 346
EcoRI GAATTC 1 cut(s) 844
EcoRII CCWGG 1 cut(s) 170
EcoT38I GRGCYC 2 cut(s) 361, 990
Esp3I CGTCTC 1 cut(s) 87
FaeI CATG 1 cut(s) 787
FaiI YATR 3 cut(s) 622, 785, 1026
FaqI GGGAC 4 cut(s) 160, 359, 721, 743
FatI CATG 1 cut(s) 783
FauI CCCGC 6 cut(s) 14, 185, 458, 567, 860, 922
FblI GTMKAC 4 cut(s) 414, 633, 702, 735
Fnu4HI GCNGC 3 cut(s) 67, 101, 193
FokI GGATG 1 cut(s) 874
FriOI GRGCYC 2 cut(s) 361, 990
Fsp4HI GCNGC 3 cut(s) 67, 101, 193
GlaI GCGC 1 cut(s) 604
GluI GCNGC 3 cut(s) 67, 101, 193
GsaI CCCAGC 1 cut(s) 108
GsuI CTGGAG 1 cut(s) 337
HaeII RGCGCY 1 cut(s) 606
HaeIII GGCC 4 cut(s) 192, 476, 639, 771
HapII CCGG 9 cut(s) 111, 361, 372, 390, 472, 556, 616, 713, 939
HgaI GACGC 1 cut(s) 619
HhaI GCGC 1 cut(s) 605
Hin1I GRCGYC 1 cut(s) 732
Hin1II CATG 1 cut(s) 787
Hin6I GCGC 1 cut(s) 603
HinP1I GCGC 1 cut(s) 603
HincII GTYRAC 3 cut(s) 415, 634, 703
HindII GTYRAC 3 cut(s) 415, 634, 703
HinfI GANTC 5 cut(s) 230, 585, 693, 709, 792
HpaII CCGG 9 cut(s) 111, 361, 372, 390, 472, 556, 616, 713, 939
HphI GGTGA 3 cut(s) 118, 160, 967
Hpy166II GTNNAC 4 cut(s) 415, 634, 703, 736
Hpy188I TCNGA 5 cut(s) 487, 584, 680, 791, 843
Hpy188III TCNNGA 6 cut(s) 295, 354, 372, 508, 751, 859
Hpy8I GTNNAC 4 cut(s) 415, 634, 703, 736
HpyAV CCTTC 6 cut(s) 193, 221, 235, 270, 295, 794
HpyCH4III ACNGT 1 cut(s) 81
HpyCH4IV ACGT 3 cut(s) 131, 522, 732
HpyCH4V TGCA 3 cut(s) 652, 718, 1018
HpyF10VI GCNNNNNNNGC 3 cut(s) 636, 658, 768
HpySE526I ACGT 3 cut(s) 131, 522, 732
Hsp92I GRCGYC 1 cut(s) 732
Hsp92II CATG 1 cut(s) 787
HspAI GCGC 1 cut(s) 603
Kpn2I TCCGGA 1 cut(s) 371
Kzo9I GATC 2 cut(s) 579, 720
LguI GCTCTTC 1 cut(s) 887
LmnI GCTCC 5 cut(s) 113, 185, 200, 314, 374
MabI ACCWGGT 1 cut(s) 170
MaeII ACGT 3 cut(s) 131, 522, 732
MalI GATC 2 cut(s) 581, 722
MbiI CCGCTC 3 cut(s) 180, 195, 867
MboI GATC 2 cut(s) 579, 720
MboII GAAGA 8 cut(s) 151, 283, 310, 809, 894, 897, 904, 927
MflI RGATCY 2 cut(s) 579, 720
MhlI GDGCHC 3 cut(s) 361, 522, 990
MluCI AATT 7 cut(s) 569, 642, 674, 823, 830, 844, 853
MlyI GAGTC 1 cut(s) 703
MroI TCCGGA 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 896
MseI TTAA 1 cut(s) 813
MspI CCGG 9 cut(s) 111, 361, 372, 390, 472, 556, 616, 713, 939
MspR9I CCNGG 8 cut(s) 112, 172, 361, 391, 472, 473, 556, 616
MvaI CCWGG 1 cut(s) 172
MvnI CGCG 2 cut(s) 453, 776
MwoI GCNNNNNNNGC 3 cut(s) 636, 658, 768
NciI CCSGG 7 cut(s) 112, 361, 391, 472, 473, 556, 616
NdeII GATC 2 cut(s) 579, 720
NlaIII CATG 1 cut(s) 787
NlaIV GGNNCC 5 cut(s) 176, 310, 347, 370, 376
NmeAIII GCCGAG 1 cut(s) 168
NspV TTCGAA 1 cut(s) 795
PciSI GCTCTTC 1 cut(s) 887
PcsI WCGNNNNNNNCGW 5 cut(s) 408, 417, 693, 801, 914
PdmI GAANNNNTTC 1 cut(s) 896
PfeI GAWTC 4 cut(s) 230, 585, 693, 792
PkrI GCNGC 3 cut(s) 68, 102, 194
PleI GAGTC 1 cut(s) 703
PmaCI CACGTG 1 cut(s) 132
PmlI CACGTG 1 cut(s) 132
PpsI GAGTC 1 cut(s) 703
Ppu21I YACGTR 1 cut(s) 132
PpuMI RGGWCCY 1 cut(s) 346
Psp124BI GAGCTC 1 cut(s) 990
Psp5II RGGWCCY 1 cut(s) 346
Psp6I CCWGG 1 cut(s) 170
PspCI CACGTG 1 cut(s) 132
PspFI CCCAGC 1 cut(s) 104
PspGI CCWGG 1 cut(s) 170
PspN4I GGNNCC 5 cut(s) 176, 310, 347, 370, 376
PspPI GGNCC 5 cut(s) 174, 346, 374, 474, 998
PspPPI RGGWCCY 1 cut(s) 346
PstI CTGCAG 2 cut(s) 654, 1020
PsuI RGATCY 2 cut(s) 579, 720
RsaI GTAC 1 cut(s) 973
RsaNI GTAC 1 cut(s) 972
SacI GAGCTC 1 cut(s) 990
SalI GTCGAC 3 cut(s) 413, 632, 701
SapI GCTCTTC 1 cut(s) 887
SaqAI TTAA 1 cut(s) 813
SatI GCNGC 3 cut(s) 67, 101, 193
Sau3AI GATC 2 cut(s) 579, 720
Sau96I GGNCC 5 cut(s) 174, 346, 374, 474, 998
SchI GAGTC 1 cut(s) 703
ScrFI CCNGG 8 cut(s) 112, 172, 361, 391, 472, 473, 556, 616
SduI GDGCHC 3 cut(s) 361, 522, 990
SexAI ACCWGGT 1 cut(s) 170
SfcI CTRYAG 2 cut(s) 650, 1016
SfuI TTCGAA 1 cut(s) 795
SgrDI CGTCGACG 1 cut(s) 632
SinI GGWCC 4 cut(s) 174, 346, 374, 998
SmaI CCCGGG 1 cut(s) 473
Sse9I AATT 7 cut(s) 569, 642, 674, 823, 830, 844, 853
SstI GAGCTC 1 cut(s) 990
StyD4I CCNGG 8 cut(s) 110, 170, 359, 389, 470, 471, 554, 614
TaaI ACNGT 1 cut(s) 81
TaiI ACGT 3 cut(s) 134, 525, 735
TaqII GACCGA 1 cut(s) 824
TasI AATT 7 cut(s) 569, 642, 674, 823, 830, 844, 853
TauI GCSGC 3 cut(s) 69, 103, 195
TfiI GAWTC 4 cut(s) 230, 585, 693, 792
Tru1I TTAA 1 cut(s) 813
Tru9I TTAA 1 cut(s) 813
TspDTI ATGAA 1 cut(s) 772
TspGWI ACGGA 3 cut(s) 21, 234, 989
TspMI CCCGGG 1 cut(s) 471
VpaK11BI GGWCC 4 cut(s) 174, 346, 374, 998
XapI RAATTY 3 cut(s) 674, 844, 853
XmaI CCCGGG 1 cut(s) 471
XmiI GTMKAC 4 cut(s) 414, 633, 702, 735
XmnI GAANNNNTTC 1 cut(s) 896
ZraI GACGTC 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.