AT2G25280

Mediator of ErbB2-driven cell motility (Memo), related (InterPro IPR002737)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011347
Physical Location & Seq
Reverse (-)
10762355 .. 10764810
2456 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25280.1

Sequence Viewer

Length: 876 bp
ATGGAGAAGATAAGACAACCAACACATGCAGGCTCTTGGTATACCGATAATCCTACAAAATTGTCATCTGATCTTGAAGAATGGCTTAATGCAACTGGTTTGACCAAATCTCCTGATGTACGAGGCGTCATTGCACCACACGCAGGTTATTCGTACTCCGGTCGTGCAGCTGCTTATGCTTTTGCAAACATAGATCCTACTAACATTTCACGGATATTTCTTCTTGGTCCATCTCACCACTTTTATACTCCAAAATGTGCCCTTTCGACTGCAACAGTTTATAAAACCCCCATTGGAAATCTACCTGTTGATGTGGAGATGATTAAGGAGATACGAGCTATGGGAAAATTTGGAATGATGGATCTACGGGTCGATGAGGCTGAGCACAGCATGGAAATGCACTTACCGTATTTGGCCAAAGTATTTGAAGGGAACAATGTGAAAGTTGTACCCATCTTGGTTGGAGCAGTAAGTCCAGAGAATGAAGCCATGTACGGTGAATTGCTTGCTAAATATGTGGACGATCCCAAGAACTTTTTCTCAGTGTCCTCTGACTTTTGCCACTGGGGCTCAAGGTTTAATTACATGCATTATGACAACACTCATGGTGCTATACACAAATCGATTGAGGCTCTAGACAAGAAGGGAATGGATATAATAGAGACAGGAGATCCTGATGCCTTTAAGAAGTATCTTCTGGAATTTGAAAACACGATTTGTGGGCGCCACCCGATCAGTATTTTTCTCCATATGCTAAAACATTCATCAAGCAAGATTAAGATCAATTTCCTGCGGTACGAGCAGTCAAGCCAATGCCAGACAATGCGAGACAGCAGTGTCAGCTATGCATCTGCTGCAGCCAAGTTGGAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Memo

Protein Analysis

291

Amino Acids

32.64

Weight (kDa)

6.35

Isoelectric Point (pI)

35.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Memo PF01875 6 - 285 4e-79 Memo-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
AasI GACNNNNNNGTC 1 cut(s) 836
Acc36I ACCTGC 1 cut(s) 134
AccB1I GGYRCC 1 cut(s) 723
AccI GTMKAC 1 cut(s) 41
AciI CCGC 1 cut(s) 793
AclWI GGATC 4 cut(s) 188, 369, 518, 665
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 2 cut(s) 347, 701
AcyI GRCGYC 2 cut(s) 126, 724
AfaI GTAC 5 cut(s) 120, 155, 450, 494, 797
AfiI CCNNNNNNNGG 1 cut(s) 143
AgsI TTSAA 3 cut(s) 77, 428, 707
AjuI GAANNNNNNNTTGG 2 cut(s) 521, 553
AluBI AGCT 3 cut(s) 170, 338, 843
AluI AGCT 3 cut(s) 170, 338, 843
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 2 cut(s) 656, 822
AlwI GGATC 4 cut(s) 188, 369, 518, 665
AoxI GGCC 1 cut(s) 414
ApeKI GCWGC 4 cut(s) 167, 170, 854, 857
ApoI RAATTY 2 cut(s) 347, 701
Asp700I GAANNNNTTC 1 cut(s) 536
AspLEI GCGC 1 cut(s) 726
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 227, 509
AvaII GGWCC 1 cut(s) 227
BaeGI GKGCMC 1 cut(s) 262
BalI TGGCCA 1 cut(s) 416
BanI GGYRCC 1 cut(s) 723
BanII GRGCYC 1 cut(s) 572
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 4 cut(s) 157, 179, 841, 869
BccI CCATC 3 cut(s) 238, 352, 461
BcgI CGANNNNNNTGC 2 cut(s) 132, 166
BcoDI GTCTC 2 cut(s) 656, 822
BfaI CTAG 1 cut(s) 635
BfmI CTRYAG 1 cut(s) 855
BfoI RGCGCY 1 cut(s) 727
BfuAI ACCTGC 1 cut(s) 134
BglI GCCNNNNNGGC 1 cut(s) 567
BisI GCNGC 4 cut(s) 168, 171, 855, 858
BlpI GCTNAGC 1 cut(s) 381
BlsI GCNGC 4 cut(s) 169, 172, 856, 859
Bme18I GGWCC 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 1 cut(s) 725
BmrI ACTGGG 1 cut(s) 574
BmsI GCATC 2 cut(s) 667, 857
BmuI ACTGGG 1 cut(s) 574
Bpu1102I GCTNAGC 1 cut(s) 381
BpuEI CTTGAG 1 cut(s) 556
Bsa29I ATCGAT 1 cut(s) 623
BsaBI GATNNNNATC 1 cut(s) 779
BsaHI GRCGYC 2 cut(s) 126, 724
BsaWI WCCGGW 1 cut(s) 158
BsaXI ACNNNNNCTCC 2 cut(s) 94, 124
Bsc4I CCNNNNNNNGG 1 cut(s) 143
Bse1I ACTGG 2 cut(s) 100, 569
Bse3DI GCAATG 1 cut(s) 129
Bse8I GATNNNNATC 1 cut(s) 779
BseCI ATCGAT 1 cut(s) 623
BseJI GATNNNNATC 1 cut(s) 779
BseLI CCNNNNNNNGG 1 cut(s) 143
BseMI GCAATG 1 cut(s) 129
BseMII CTCAG 2 cut(s) 372, 555
BseNI ACTGG 2 cut(s) 100, 569
BseSI GKGCMC 1 cut(s) 262
BseXI GCAGC 4 cut(s) 157, 179, 841, 869
BsgI GTGCAG 1 cut(s) 186
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 1 cut(s) 416
BshNI GGYRCC 1 cut(s) 723
BshVI ATCGAT 1 cut(s) 623
BsiEI CGRYCG 1 cut(s) 163
BsiHKAI GWGCWC 1 cut(s) 387
BsiSI CCGG 1 cut(s) 159
BslI CCNNNNNNNGG 1 cut(s) 143
BsmAI GTCTC 2 cut(s) 656, 822
BsnI GGCC 1 cut(s) 416
Bsp1286I GDGCHC 3 cut(s) 262, 387, 572
Bsp143I GATC 7 cut(s) 70, 193, 361, 523, 670, 732, 780
Bsp1720I GCTNAGC 1 cut(s) 381
BspACI CCGC 1 cut(s) 793
BspANI GGCC 1 cut(s) 416
BspCNI CTCAG 2 cut(s) 373, 554
BspDI ATCGAT 1 cut(s) 623
BspLI GGNNCC 1 cut(s) 725
BspMAI CTGCAG 1 cut(s) 859
BspMI ACCTGC 1 cut(s) 134
BspPI GGATC 4 cut(s) 188, 369, 518, 665
BspT107I GGYRCC 1 cut(s) 723
BsrDI GCAATG 1 cut(s) 129
BsrI ACTGG 2 cut(s) 100, 569
BssMI GATC 7 cut(s) 70, 193, 361, 523, 670, 732, 780
BssNAI GTATAC 1 cut(s) 42
BssNI GRCGYC 2 cut(s) 126, 724
Bst1107I GTATAC 1 cut(s) 42
Bst4CI ACNGT 3 cut(s) 277, 408, 497
BstACI GRCGYC 2 cut(s) 126, 724
BstAPI GCANNNNNTGC 1 cut(s) 854
BstC8I GCNNGC 2 cut(s) 31, 507
BstDEI CTNAG 2 cut(s) 381, 541
BstH2I RGCGCY 1 cut(s) 727
BstHHI GCGC 1 cut(s) 726
BstKTI GATC 7 cut(s) 73, 196, 364, 526, 673, 735, 783
BstMAI GTCTC 2 cut(s) 656, 822
BstMBI GATC 7 cut(s) 70, 193, 361, 523, 670, 732, 780
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 6 cut(s) 140, 176, 567, 799, 840, 854
BstNSI RCATGY 2 cut(s) 29, 589
BstSFI CTRYAG 1 cut(s) 855
BstSLI GKGCMC 1 cut(s) 262
BstV1I GCAGC 4 cut(s) 157, 179, 841, 869
BstX2I RGATCY 3 cut(s) 193, 361, 670
BstYI RGATCY 3 cut(s) 193, 361, 670
BstZ17I GTATAC 1 cut(s) 42
Bsu15I ATCGAT 1 cut(s) 623
BsuRI GGCC 1 cut(s) 416
BsuTUI ATCGAT 1 cut(s) 623
BtsI GCAGTG 1 cut(s) 841
BtsIMutI CAGTG 3 cut(s) 549, 562, 841
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 2 cut(s) 31, 507
CfoI GCGC 1 cut(s) 726
Cfr13I GGNCC 1 cut(s) 227
ClaI ATCGAT 1 cut(s) 623
CseI GACGC 1 cut(s) 115
Csp6I GTAC 5 cut(s) 119, 154, 449, 493, 796
CviAII CATG 6 cut(s) 26, 391, 490, 586, 605, 873
CviQI GTAC 5 cut(s) 119, 154, 449, 493, 796
DdeI CTNAG 2 cut(s) 381, 541
DinI GGCGCC 1 cut(s) 725
DpnI GATC 7 cut(s) 72, 195, 363, 525, 672, 734, 782
DpnII GATC 7 cut(s) 70, 193, 361, 523, 670, 732, 780
DrdI GACNNNNNNGTC 1 cut(s) 836
DseDI GACNNNNNNGTC 1 cut(s) 836
EaeI YGGCCR 1 cut(s) 414
Eco24I GRGCYC 1 cut(s) 572
Eco47I GGWCC 1 cut(s) 227
EcoT22I ATGCAT 2 cut(s) 591, 850
EcoT38I GRGCYC 1 cut(s) 572
EgeI GGCGCC 1 cut(s) 725
EheI GGCGCC 1 cut(s) 725
FaeI CATG 6 cut(s) 29, 394, 493, 589, 608, 876
FalI AAGNNNNNCTT 2 cut(s) 69, 101
FatI CATG 6 cut(s) 25, 390, 489, 585, 604, 872
FauNDI CATATG 1 cut(s) 750
FblI GTMKAC 1 cut(s) 41
Fnu4HI GCNGC 4 cut(s) 168, 171, 855, 858
FriOI GRGCYC 1 cut(s) 572
Fsp4HI GCNGC 4 cut(s) 168, 171, 855, 858
FspBI CTAG 1 cut(s) 635
GlaI GCGC 1 cut(s) 725
GluI GCNGC 4 cut(s) 168, 171, 855, 858
HaeII RGCGCY 1 cut(s) 727
HaeIII GGCC 1 cut(s) 416
HapII CCGG 1 cut(s) 159
HgaI GACGC 1 cut(s) 115
HhaI GCGC 1 cut(s) 726
Hin1I GRCGYC 2 cut(s) 126, 724
Hin1II CATG 6 cut(s) 29, 394, 493, 589, 608, 876
Hin6I GCGC 1 cut(s) 724
HinP1I GCGC 1 cut(s) 724
HpaII CCGG 1 cut(s) 159
HphI GGTGA 2 cut(s) 227, 509
Hpy166II GTNNAC 2 cut(s) 42, 520
Hpy188I TCNGA 2 cut(s) 70, 553
Hpy188III TCNNGA 6 cut(s) 74, 113, 476, 635, 674, 698
Hpy8I GTNNAC 2 cut(s) 42, 520
HpyAV CCTTC 2 cut(s) 422, 637
HpyCH4III ACNGT 3 cut(s) 277, 408, 497
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 176, 567, 799, 840, 854
HpyF3I CTNAG 2 cut(s) 381, 541
Hsp92I GRCGYC 2 cut(s) 126, 724
Hsp92II CATG 6 cut(s) 29, 394, 493, 589, 608, 876
HspAI GCGC 1 cut(s) 724
KasI GGCGCC 1 cut(s) 723
Kzo9I GATC 7 cut(s) 70, 193, 361, 523, 670, 732, 780
LmnI GCTCC 1 cut(s) 464
Lsp1109I GCAGC 4 cut(s) 157, 179, 841, 869
LweI GCATC 2 cut(s) 667, 857
MaeI CTAG 1 cut(s) 635
MalI GATC 7 cut(s) 72, 195, 363, 525, 672, 734, 782
MboI GATC 7 cut(s) 70, 193, 361, 523, 670, 732, 780
MboII GAAGA 4 cut(s) 19, 89, 212, 686
MflI RGATCY 3 cut(s) 193, 361, 670
MhlI GDGCHC 3 cut(s) 262, 387, 572
MlsI TGGCCA 1 cut(s) 416
MluCI AATT 6 cut(s) 59, 347, 500, 580, 701, 784
MluNI TGGCCA 1 cut(s) 416
Mly113I GGCGCC 1 cut(s) 724
MmeI TCCRAC 2 cut(s) 442, 846
MnlI CCTC 4 cut(s) 116, 370, 559, 622
Mox20I TGGCCA 1 cut(s) 416
Mph1103I ATGCAT 2 cut(s) 591, 850
MroXI GAANNNNTTC 1 cut(s) 536
MscI TGGCCA 1 cut(s) 416
MseI TTAA 5 cut(s) 87, 324, 579, 684, 777
MslI CAYNNNNRTG 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 416
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 1 cut(s) 159
MwoI GCNNNNNNNGC 6 cut(s) 140, 176, 567, 799, 840, 854
NarI GGCGCC 1 cut(s) 724
NdeI CATATG 1 cut(s) 750
NdeII GATC 7 cut(s) 70, 193, 361, 523, 670, 732, 780
NlaIII CATG 6 cut(s) 29, 394, 493, 589, 608, 876
NlaIV GGNNCC 1 cut(s) 725
NsiI ATGCAT 2 cut(s) 591, 850
NspI RCATGY 2 cut(s) 29, 589
PdmI GAANNNNTTC 1 cut(s) 536
PkrI GCNGC 4 cut(s) 169, 172, 856, 859
PluTI GGCGCC 1 cut(s) 727
PsiI TTATAA 1 cut(s) 282
PspN4I GGNNCC 1 cut(s) 725
PspPI GGNCC 1 cut(s) 227
PstI CTGCAG 1 cut(s) 859
PsuI RGATCY 3 cut(s) 193, 361, 670
PvuII CAGCTG 1 cut(s) 170
RsaI GTAC 5 cut(s) 120, 155, 450, 494, 797
RsaNI GTAC 5 cut(s) 119, 154, 449, 493, 796
RseI CAYNNNNRTG 1 cut(s) 395
SaqAI TTAA 5 cut(s) 87, 324, 579, 684, 777
SatI GCNGC 4 cut(s) 168, 171, 855, 858
Sau3AI GATC 7 cut(s) 70, 193, 361, 523, 670, 732, 780
Sau96I GGNCC 1 cut(s) 227
SduI GDGCHC 3 cut(s) 262, 387, 572
SetI ASST 6 cut(s) 148, 172, 307, 340, 578, 845
SfaNI GCATC 2 cut(s) 667, 857
SfcI CTRYAG 1 cut(s) 855
SfoI GGCGCC 1 cut(s) 725
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 571
SmoI CTYRAG 1 cut(s) 571
Sse9I AATT 6 cut(s) 59, 347, 500, 580, 701, 784
SsiI CCGC 1 cut(s) 793
SspDI GGCGCC 1 cut(s) 723
SspMI CTAG 1 cut(s) 635
TaaI ACNGT 3 cut(s) 277, 408, 497
TaqI TCGA 3 cut(s) 266, 372, 623
TasI AATT 6 cut(s) 59, 347, 500, 580, 701, 784
Tru1I TTAA 5 cut(s) 87, 324, 579, 684, 777
Tru9I TTAA 5 cut(s) 87, 324, 579, 684, 777
TscAI CASTG 3 cut(s) 549, 569, 841
TseI GCWGC 4 cut(s) 167, 170, 854, 857
TspDTI ATGAA 2 cut(s) 498, 753
TspGWI ACGGA 1 cut(s) 226
TspRI CASTG 3 cut(s) 549, 569, 841
VpaK11BI GGWCC 1 cut(s) 227
XapI RAATTY 2 cut(s) 347, 701
XbaI TCTAGA 1 cut(s) 634
XceI RCATGY 2 cut(s) 29, 589
XmiI GTMKAC 1 cut(s) 41
XmnI GAANNNNTTC 1 cut(s) 536
XspI CTAG 1 cut(s) 635
Zsp2I ATGCAT 2 cut(s) 591, 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.