MD08G1152600.v1.1

Memo-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011347
Physical Location & Seq
Reverse (-)
16415248 .. 16418304
3057 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1152600.v1.1.491

Sequence Viewer

Length: 876 bp
ATGGAGAAAGTCAGAAGAGCATCGCACGCCGGTTCTTGGTACACCGACAATCCTAAGAAGTTATCAGAAGAGATTGAAGGATGGCTGAGAGCATCTGGGCTCACTAAATCTCCTCATGTTCGAGGTGTCATTGCTCCGCATGCAGGGTATTCGTATTCGGGTCGTGCCGCTGCCTATGCATTTGGCAACATTGACCCAACAAACATTACGCGGGTTTTCCTGCTCGGTCCATCTCACCACCATTACACTCCAAAGTGTGCTCTTTCGACGGCCACGGTTTACAAGACCCCCATTGGGGACCTACCTATTGATTTGGAAGTGATTGAGGAGCTTAAAGCTTGTGGAAAATTTGAACTGATGGATATTTGCGTTGATGAGGCTGAACATAGCATGGAAATGCACTTGCCATATCTTGCTAAAGTGTTTGAGGGGCACCAAGTTAAAGTTGTACCCATTTTGGTTGGTGCTCTTAAAGCTGAAAGTGAAGCCATGTATGGACAGTTCCTTGCGAAATATGTGGATGATCCACAAAACTTCTTCTCTGTGTCGTCAGATTTTTGTCATTGGGGGTCTCGGTTCAAATACATGCACTATGACAAGAAACATGGTGCCATACACAAATCTATTGAGGCTTTGGACAGGATGGGAATGGATATAATAGAAACTGGAAATCCAGATGCGTTCAAGCAGTATCTGTCAGAGTTTGATAACACCATTTGTGGGCGTCACCCAATTAGCGTTTTTCTGCATATGCTGAGGAATTGCTCAATAAACATAAAGATCAGTTTTCTCCGATACGAGCAGTCAAGCCAGTGCAAAACTACGAGGGACAGCAGCGTAAGTTATGCATCTGCGGCAGCAAAGGTAGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Memo

Protein Analysis

292

Amino Acids

32.62

Weight (kDa)

6.73

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Memo PF01875 7 - 285 9.2e-80 Memo-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 432, 608
AccII CGCG 1 cut(s) 211
AciI CCGC 4 cut(s) 137, 168, 211, 854
AclWI GGATC 1 cut(s) 518
AcoI YGGCCR 1 cut(s) 270
AcsI RAATTY 1 cut(s) 347
AcyI GRCGYC 1 cut(s) 724
AfaI GTAC 2 cut(s) 41, 450
AfiI CCNNNNNNNGG 5 cut(s) 36, 143, 294, 295, 720
AgsI TTSAA 4 cut(s) 77, 353, 580, 685
AluBI AGCT 3 cut(s) 331, 338, 476
AluI AGCT 3 cut(s) 331, 338, 476
Alw21I GWGCWC 2 cut(s) 262, 469
Alw26I GTCTC 1 cut(s) 576
AlwI GGATC 1 cut(s) 518
AlwNI CAGNNNCTG 1 cut(s) 694
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 3 cut(s) 170, 834, 857
ApoI RAATTY 1 cut(s) 347
AspS9I GGNCC 2 cut(s) 227, 298
AsuHPI GGTGA 2 cut(s) 227, 719
AvaII GGWCC 2 cut(s) 227, 298
BaeGI GKGCMC 1 cut(s) 435
BanI GGYRCC 2 cut(s) 432, 608
BanII GRGCYC 1 cut(s) 102
Bbv12I GWGCWC 2 cut(s) 262, 469
BbvCI CCTCAGC 1 cut(s) 755
BbvI GCAGC 3 cut(s) 157, 846, 869
BccI CCATC 4 cut(s) 75, 238, 352, 637
BceAI ACGGC 1 cut(s) 285
BcgI CGANNNNNNTGC 2 cut(s) 132, 166
BcoDI GTCTC 1 cut(s) 576
BisI GCNGC 5 cut(s) 168, 171, 835, 855, 858
BlsI GCNGC 5 cut(s) 169, 172, 836, 856, 859
Bme18I GGWCC 2 cut(s) 227, 298
BmgT120I GGNCC 2 cut(s) 227, 298
BmiI GGNNCC 3 cut(s) 299, 434, 610
BmsI GCATC 5 cut(s) 29, 101, 667, 857, 859
Bpu10I CCTNAGC 1 cut(s) 755
BsaHI GRCGYC 1 cut(s) 724
BsaI GGTCTC 1 cut(s) 576
BsaJI CCNNGG 1 cut(s) 273
BsaXI ACNNNNNCTCC 2 cut(s) 94, 124
Bsc4I CCNNNNNNNGG 5 cut(s) 36, 143, 294, 295, 720
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 2 cut(s) 670, 811
Bse3DI GCAATG 1 cut(s) 129
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 3 cut(s) 86, 526, 648
BseLI CCNNNNNNNGG 5 cut(s) 36, 143, 294, 295, 720
BseMI GCAATG 1 cut(s) 129
BseMII CTCAG 2 cut(s) 77, 746
BseNI ACTGG 2 cut(s) 670, 811
BseRI GAGGAG 2 cut(s) 102, 341
BseSI GKGCMC 1 cut(s) 435
BseXI GCAGC 3 cut(s) 157, 846, 869
Bsh1236I CGCG 1 cut(s) 211
BshFI GGCC 1 cut(s) 272
BshNI GGYRCC 2 cut(s) 432, 608
BsiHKAI GWGCWC 2 cut(s) 262, 469
BsiSI CCGG 1 cut(s) 30
BslFI GGGAC 2 cut(s) 311, 842
BslI CCNNNNNNNGG 5 cut(s) 36, 143, 294, 295, 720
BsmAI GTCTC 1 cut(s) 576
BsmFI GGGAC 2 cut(s) 311, 842
BsnI GGCC 1 cut(s) 272
Bso31I GGTCTC 1 cut(s) 576
Bsp1286I GDGCHC 4 cut(s) 102, 262, 435, 469
Bsp143I GATC 2 cut(s) 523, 780
BspACI CCGC 4 cut(s) 137, 168, 211, 854
BspANI GGCC 1 cut(s) 272
BspCNI CTCAG 2 cut(s) 78, 747
BspFNI CGCG 1 cut(s) 211
BspLI GGNNCC 3 cut(s) 299, 434, 610
BspPI GGATC 1 cut(s) 518
BspQI GCTCTTC 1 cut(s) 10
BspT107I GGYRCC 2 cut(s) 432, 608
BspTNI GGTCTC 1 cut(s) 576
BsrDI GCAATG 1 cut(s) 129
BsrFI RCCGGY 1 cut(s) 29
BsrI ACTGG 2 cut(s) 670, 811
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 2 cut(s) 523, 780
BssNI GRCGYC 1 cut(s) 724
Bst4CI ACNGT 2 cut(s) 277, 501
Bst6I CTCTTC 2 cut(s) 10, 63
BstACI GRCGYC 1 cut(s) 724
BstC8I GCNNGC 2 cut(s) 27, 141
BstDEI CTNAG 3 cut(s) 54, 86, 755
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 3 cut(s) 86, 526, 648
BstFNI CGCG 1 cut(s) 211
BstKTI GATC 2 cut(s) 526, 783
BstMAI GTCTC 1 cut(s) 576
BstMBI GATC 2 cut(s) 523, 780
BstMWI GCNNNNNNNGC 5 cut(s) 26, 140, 176, 473, 854
BstNSI RCATGY 2 cut(s) 143, 589
BstSLI GKGCMC 1 cut(s) 435
BstUI CGCG 1 cut(s) 211
BstV1I GCAGC 3 cut(s) 157, 846, 869
BsuRI GGCC 1 cut(s) 272
BtgI CCRYGG 1 cut(s) 273
BtgZI GCGATG 1 cut(s) 6
BtsCI GGATG 3 cut(s) 86, 526, 648
BtsIMutI CAGTG 1 cut(s) 818
Cac8I GCNNGC 2 cut(s) 27, 141
CaiI CAGNNNCTG 1 cut(s) 694
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 2 cut(s) 227, 298
CseI GACGC 1 cut(s) 713
Csp6I GTAC 2 cut(s) 40, 449
CviAII CATG 6 cut(s) 116, 140, 391, 490, 586, 605
CviQI GTAC 2 cut(s) 40, 449
DdeI CTNAG 3 cut(s) 54, 86, 755
DpnI GATC 2 cut(s) 525, 782
DpnII GATC 2 cut(s) 523, 780
EaeI YGGCCR 1 cut(s) 270
Eam1104I CTCTTC 2 cut(s) 10, 63
EarI CTCTTC 2 cut(s) 10, 63
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 576
Eco47I GGWCC 2 cut(s) 227, 298
EcoO109I RGGNCCY 1 cut(s) 298
EcoT22I ATGCAT 2 cut(s) 181, 850
EcoT38I GRGCYC 1 cut(s) 102
FaeI CATG 6 cut(s) 119, 143, 394, 493, 589, 608
FaqI GGGAC 2 cut(s) 311, 842
FatI CATG 6 cut(s) 115, 139, 390, 489, 585, 604
FauI CCCGC 1 cut(s) 204
FauNDI CATATG 1 cut(s) 750
Fnu4HI GCNGC 5 cut(s) 168, 171, 835, 855, 858
FokI GGATG 3 cut(s) 93, 533, 655
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 5 cut(s) 168, 171, 835, 855, 858
GluI GCNGC 5 cut(s) 168, 171, 835, 855, 858
HaeIII GGCC 1 cut(s) 272
HapII CCGG 1 cut(s) 30
HgaI GACGC 1 cut(s) 713
Hin1I GRCGYC 1 cut(s) 724
Hin1II CATG 6 cut(s) 119, 143, 394, 493, 589, 608
HindIII AAGCTT 1 cut(s) 336
HpaII CCGG 1 cut(s) 30
HphI GGTGA 2 cut(s) 227, 719
Hpy166II GTNNAC 2 cut(s) 42, 280
Hpy188I TCNGA 5 cut(s) 14, 67, 553, 700, 794
Hpy188III TCNNGA 1 cut(s) 674
Hpy8I GTNNAC 2 cut(s) 42, 280
Hpy99I CGWCG 1 cut(s) 271
HpyAV CCTTC 1 cut(s) 71
HpyCH4III ACNGT 2 cut(s) 277, 501
HpyCH4V TGCA 7 cut(s) 143, 179, 400, 589, 748, 816, 848
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 140, 176, 473, 854
HpyF3I CTNAG 3 cut(s) 54, 86, 755
Hsp92I GRCGYC 1 cut(s) 724
Hsp92II CATG 6 cut(s) 119, 143, 394, 493, 589, 608
Kzo9I GATC 2 cut(s) 523, 780
LguI GCTCTTC 1 cut(s) 10
LmnI GCTCC 2 cut(s) 139, 328
LpnPI CCDG 8 cut(s) 43, 81, 129, 233, 625, 651, 687, 824
Lsp1109I GCAGC 3 cut(s) 157, 846, 869
LweI GCATC 5 cut(s) 29, 101, 667, 857, 859
MaeIII GTNAC 1 cut(s) 725
MalI GATC 2 cut(s) 525, 782
MboI GATC 2 cut(s) 523, 780
MboII GAAGA 3 cut(s) 27, 80, 529
MhlI GDGCHC 4 cut(s) 102, 262, 435, 469
MluCI AATT 3 cut(s) 347, 732, 760
MnlI CCTC 8 cut(s) 116, 123, 319, 370, 421, 622, 750, 819
Mph1103I ATGCAT 2 cut(s) 181, 850
MseI TTAA 3 cut(s) 333, 441, 471
MslI CAYNNNNRTG 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 1 cut(s) 30
MvnI CGCG 1 cut(s) 211
MwoI GCNNNNNNNGC 5 cut(s) 26, 140, 176, 473, 854
NdeI CATATG 1 cut(s) 750
NdeII GATC 2 cut(s) 523, 780
NlaIII CATG 6 cut(s) 119, 143, 394, 493, 589, 608
NlaIV GGNNCC 3 cut(s) 299, 434, 610
NmuCI GTSAC 1 cut(s) 725
NsiI ATGCAT 2 cut(s) 181, 850
NspI RCATGY 2 cut(s) 143, 589
PaeI GCATGC 1 cut(s) 143
PciSI GCTCTTC 1 cut(s) 10
PkrI GCNGC 5 cut(s) 169, 172, 836, 856, 859
PpuMI RGGWCCY 1 cut(s) 298
Psp5II RGGWCCY 1 cut(s) 298
PspN4I GGNNCC 3 cut(s) 299, 434, 610
PspPI GGNCC 2 cut(s) 227, 298
PspPPI RGGWCCY 1 cut(s) 298
PsrI GAACNNNNNNTAC 2 cut(s) 485, 517
PstNI CAGNNNCTG 1 cut(s) 694
RsaI GTAC 2 cut(s) 41, 450
RsaNI GTAC 2 cut(s) 40, 449
RseI CAYNNNNRTG 1 cut(s) 395
SapI GCTCTTC 1 cut(s) 10
SaqAI TTAA 3 cut(s) 333, 441, 471
SatI GCNGC 5 cut(s) 168, 171, 835, 855, 858
Sau3AI GATC 2 cut(s) 523, 780
Sau96I GGNCC 2 cut(s) 227, 298
SduI GDGCHC 4 cut(s) 102, 262, 435, 469
SetI ASST 7 cut(s) 127, 303, 307, 333, 340, 478, 867
SfaNI GCATC 5 cut(s) 29, 101, 667, 857, 859
SinI GGWCC 2 cut(s) 227, 298
SmiMI CAYNNNNRTG 1 cut(s) 395
SphI GCATGC 1 cut(s) 143
Sse9I AATT 3 cut(s) 347, 732, 760
SsiI CCGC 4 cut(s) 137, 168, 211, 854
TaaI ACNGT 2 cut(s) 277, 501
TaqI TCGA 2 cut(s) 121, 266
TaqII GACCGA 1 cut(s) 215
TasI AATT 3 cut(s) 347, 732, 760
TauI GCSGC 2 cut(s) 170, 857
Tru1I TTAA 3 cut(s) 333, 441, 471
Tru9I TTAA 3 cut(s) 333, 441, 471
TscAI CASTG 1 cut(s) 818
TseFI GTSAC 1 cut(s) 725
TseI GCWGC 3 cut(s) 170, 834, 857
Tsp45I GTSAC 1 cut(s) 725
TspRI CASTG 1 cut(s) 818
VpaK11BI GGWCC 2 cut(s) 227, 298
XapI RAATTY 1 cut(s) 347
XceI RCATGY 2 cut(s) 143, 589
Zsp2I ATGCAT 2 cut(s) 181, 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.