AT2G25530

AFG1-like ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0007203
Physical Location & Seq
Forward (+)
10863059 .. 10866910
3852 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25530.2

Sequence Viewer

Length: 1968 bp
ATGTCACGGACCGGACCACATCGGGTTTGGCAGATTTGGCATCCTAGTTTTGACATCTCTACCAAACCCACTAAAGAAATCTACACGGACACTAATAAGCCAAATCAGCAATACGACAAACGGTCCATATTCCACCACCTAAACCGTGATCGTAAAGCTAGTCTCCAGAGAAGAAACCACAATCGGAGTCTGAAGCAACAAATTATGCCTAATGTTTCTCAGATCTCTACTGTTCTACGTCTAGCGTTTTCTTGTAAAAGAAGACTCTCCAATGGCTTACACTTCGTTGCTCGTTCCTATTCCACCTCTGATCCTCCCAAACAACCATCAGGTCCACTGACGAATTACTCTAAGTTAGTGGAGCAAGGGAGGTTACAACATGATCCATACCAAGAGAAAGTTGTTTCGGCATTCGAAAATTTGTTTGGAAGATTGGAACATTTCGAGAAGCAAATGGAGGATTATCATGTGAGATTAGCAGAGTGGGAGAAGAAGAGAGAGGAGGAACGAAGAAAGCTAATGGTGGAAGAAGCTGAGAAGAAGGAAGATGATGGAATGTGGGCATCTGTGAATAAGCAGGGACAAAAGCTTTTGGGAAGATGGGTATTAGGGCGGAGACAGATGAATGTAGAACCTGGAGTTGGTAAATGGGTATCGTACCTTAACCGAGAGAGAAAATTGGACTCGATTGTCGGTTCTCGTCCCGCGGTACCACCAGCTCCAAAAGGATTATATATCTATGGAAATGTAGGATGTGGCAAGACTATGTTGATGGATATGTTTTATGGTGCTACTGATGGGATCATTAGACATAGACAACGGTTTCACTTTCATGAGGCTATGCTGAAGATAAATGAACAAATGCACAAGTATTGGAAGGAGAATGGTGCTGAGAAGCCAATGCAATATAGCATTTCGAGCTGGATTATGAATCTTCCTGTTGATGAAAAAGTTAAGGAATGGCTAGCAGGAGAAGAATTTTATAAGCAGCAACTCCAAATGAAACACATTCTTCCAGCTGTGGCTGATAAGTTTCTCGTTGATCAGCAATCGAGTAAAAAAGGAGCTAGCATCCTCTGCTTTGATGAGATTCAGACAGTTGATGTATTTGCTATTGTGGCCTTATCTGGAATTATGAGCAGATTGTTGACTACTGGAACTGTTCTTGTGGCCACCAGTAACCGAGCACCACGGGAGTTGAATCAGGACGGAATGCAGAAGGAGATATTTGATAAGTTTATCTCTAAATTGGAGAAACATTGTGAAATAATCTCAATTGGAAGTGAAGTAGATTACCGCCGAGTTGCAGCAAAAAATTCGGCTGAAAATGTTCACTACTTATGGCCTTTGAATGATGCTGTTCTGGAAGAATTTGAGAAAATGTGGCGTCAGGTCACTGACCAGTACGGTGGAGAAATAACTTCGGCGACTCTCCCAGTGATGTTTGGAAGAACAGTGGAAGTTCCTGAAAGCTGTAGTGGAGTGGCAAGGTTCACATTCGAGTATCTATGTGGTCGACCGGTAGGTGCAGCAGATTATATCGCAGTGGCCAAAAACTATCATACAATTTTTATATCTGATATTCCAGCAATGAGCATGGAGATACGCGACAAGGCACGTAGGTTTATAACCCTTGTAGATGAGTTATACAACCATCATTGTTGTCTTGTCTCAAGCGCGGAGACAGCAATTGATGAGTTGTTTCAAGGAACTGCAGAGGGAACGCTCTTTGACCTGGAAAGCTTCCAGTTTGAGACAGAGACGGAAGATTCAAGATTAAGGCGAGATGTGCTTGCAGAAGGTAGCATAAGTGCAGCTGGCTCGCCTTCTTCGATCGTATCAATGCTTTCTGGTGAAGAGGAAATGTTTGCTTTTGCTCGAGCTGCGTCTCGGTTAATTGAGATGCAGACTCCGTTGTACCTTGAAGGAGTTCGTTTCTTGCATCCTTACTTCCATCAGCAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

655

Amino Acids

75.41

Weight (kDa)

7.98

Isoelectric Point (pI)

48.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 229 - 533 2.1e-52 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 984, 1628
AasI GACNNNNNNGTC 1 cut(s) 689
Acc65I GGTACC 1 cut(s) 709
AccB1I GGYRCC 1 cut(s) 709
AccI GTMKAC 1 cut(s) 1516
AccII CGCG 3 cut(s) 707, 1608, 1679
AciI CCGC 5 cut(s) 613, 705, 707, 1297, 1679
AclWI GGATC 3 cut(s) 305, 377, 809
AcoI YGGCCR 2 cut(s) 1170, 1548
AcsI RAATTY 4 cut(s) 418, 977, 1315, 1370
AcuI CTGAAG 2 cut(s) 212, 866
AcyI GRCGYC 1 cut(s) 1387
AfaI GTAC 4 cut(s) 659, 711, 1406, 1919
AfiI CCNNNNNNNGG 1 cut(s) 641
AgeI ACCGGT 1 cut(s) 1519
AgsI TTSAA 5 cut(s) 1201, 1351, 1706, 1773, 1925
AhdI GACNNNNNGTC 1 cut(s) 121
AjnI CCWGG 2 cut(s) 634, 1734
AjuI GAANNNNNNNTTGG 4 cut(s) 408, 440, 624, 656
Alw21I GWGCWC 1 cut(s) 1189
Alw26I GTCTC 7 cut(s) 167, 610, 1675, 1676, 1748, 1754, 1893
AlwI GGATC 3 cut(s) 305, 377, 809
Ama87I CYCGRG 1 cut(s) 1878
AoxI GGCC 4 cut(s) 1119, 1170, 1343, 1548
ApeKI GCWGC 5 cut(s) 988, 1307, 1529, 1814, 1883
ApoI RAATTY 4 cut(s) 418, 977, 1315, 1370
AsiGI ACCGGT 1 cut(s) 1519
Asp700I GAANNNNTTC 2 cut(s) 1329, 1929
Asp718I GGTACC 1 cut(s) 709
AspLEI GCGC 1 cut(s) 1679
AspS9I GGNCC 4 cut(s) 9, 14, 123, 332
AsuHPI GGTGA 1 cut(s) 1865
AsuII TTCGAA 1 cut(s) 414
AsuNHI GCTAGC 2 cut(s) 964, 1067
AvaI CYCGRG 1 cut(s) 1878
AvaII GGWCC 4 cut(s) 9, 14, 123, 332
BalI TGGCCA 2 cut(s) 1172, 1550
BanI GGYRCC 1 cut(s) 709
BbsI GAAGAC 1 cut(s) 268
Bbv12I GWGCWC 1 cut(s) 1189
BbvI GCAGC 5 cut(s) 1000, 1319, 1541, 1826, 1870
BccI CCATC 7 cut(s) 334, 545, 594, 766, 791, 1662, 1962
BcgI CGANNNNNNTGC 4 cut(s) 1299, 1333, 1803, 1837
BciT130I CCWGG 2 cut(s) 636, 1736
BclI TGATCA 1 cut(s) 1042
BcoDI GTCTC 7 cut(s) 167, 610, 1675, 1676, 1748, 1754, 1893
BfaI CTAG 5 cut(s) 45, 159, 242, 965, 1068
BfmI CTRYAG 2 cut(s) 1474, 1713
BglII AGATCT 1 cut(s) 222
BisI GCNGC 5 cut(s) 989, 1308, 1530, 1815, 1884
BlsI GCNGC 5 cut(s) 990, 1309, 1531, 1816, 1885
Bme1390I CCNGG 2 cut(s) 636, 1736
Bme18I GGWCC 4 cut(s) 9, 14, 123, 332
BmeRI GACNNNNNGTC 1 cut(s) 121
BmeT110I CYCGRG 1 cut(s) 1878
BmgT120I GGNCC 4 cut(s) 9, 14, 123, 332
BmiI GGNNCC 1 cut(s) 711
BmrFI CCNGG 2 cut(s) 636, 1736
BmrI ACTGGG 1 cut(s) 1430
BmsI GCATC 6 cut(s) 49, 572, 1080, 1345, 1893, 1951
BmtI GCTAGC 2 cut(s) 968, 1071
BmuI ACTGGG 1 cut(s) 1430
BpiI GAAGAC 1 cut(s) 268
BpmI CTGGAG 2 cut(s) 149, 657
Bpu14I TTCGAA 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 1657
BsaAI YACGTR 1 cut(s) 1619
BsaHI GRCGYC 1 cut(s) 1387
BsaJI CCNNGG 2 cut(s) 705, 1190
BsaWI WCCGGW 2 cut(s) 11, 1519
Bsc4I CCNNNNNNNGG 1 cut(s) 641
Bse118I RCCGGY 1 cut(s) 1519
Bse1I ACTGG 5 cut(s) 1159, 1176, 1402, 1436, 1747
Bse3DI GCAATG 1 cut(s) 1596
BseBI CCWGG 2 cut(s) 636, 1736
BseDI CCNNGG 2 cut(s) 705, 1190
BseGI GGATG 4 cut(s) 40, 758, 1071, 1942
BseLI CCNNNNNNNGG 1 cut(s) 641
BseMI GCAATG 1 cut(s) 1596
BseMII CTCAG 3 cut(s) 233, 525, 882
BseNI ACTGG 5 cut(s) 1159, 1176, 1402, 1436, 1747
BseRI GAGGAG 1 cut(s) 515
BseXI GCAGC 5 cut(s) 1000, 1319, 1541, 1826, 1870
BsgI GTGCAG 2 cut(s) 1548, 1833
Bsh1236I CGCG 3 cut(s) 707, 1608, 1679
Bsh1285I CGRYCG 2 cut(s) 1520, 1836
BshFI GGCC 4 cut(s) 1121, 1172, 1345, 1550
BshNI GGYRCC 1 cut(s) 709
BshTI ACCGGT 1 cut(s) 1519
BsiEI CGRYCG 2 cut(s) 1520, 1836
BsiHKAI GWGCWC 1 cut(s) 1189
BsiHKCI CYCGRG 1 cut(s) 1878
BsiSI CCGG 2 cut(s) 12, 1520
BslFI GGGAC 2 cut(s) 594, 687
BslI CCNNNNNNNGG 1 cut(s) 641
BsmAI GTCTC 7 cut(s) 167, 610, 1675, 1676, 1748, 1754, 1893
BsmBI CGTCTC 2 cut(s) 1754, 1893
BsmFI GGGAC 2 cut(s) 594, 687
BsmI GAATGC 2 cut(s) 410, 1218
BsnI GGCC 4 cut(s) 1121, 1172, 1345, 1550
BsoBI CYCGRG 1 cut(s) 1878
Bsp119I TTCGAA 1 cut(s) 414
Bsp1286I GDGCHC 1 cut(s) 1189
Bsp143I GATC 7 cut(s) 148, 222, 310, 382, 801, 1042, 1833
BspACI CCGC 5 cut(s) 613, 705, 707, 1297, 1679
BspANI GGCC 4 cut(s) 1121, 1172, 1345, 1550
BspCNI CTCAG 3 cut(s) 232, 526, 883
BspFNI CGCG 3 cut(s) 707, 1608, 1679
BspHI TCATGA 1 cut(s) 832
BspLI GGNNCC 1 cut(s) 711
BspMAI CTGCAG 1 cut(s) 1717
BspOI GCTAGC 2 cut(s) 968, 1071
BspPI GGATC 3 cut(s) 305, 377, 809
BspT104I TTCGAA 1 cut(s) 414
BspT107I GGYRCC 1 cut(s) 709
BsrDI GCAATG 1 cut(s) 1596
BsrFI RCCGGY 1 cut(s) 1519
BsrI ACTGG 5 cut(s) 1159, 1176, 1402, 1436, 1747
BssAI RCCGGY 1 cut(s) 1519
BssECI CCNNGG 2 cut(s) 705, 1190
BssMI GATC 7 cut(s) 148, 222, 310, 382, 801, 1042, 1833
BssNI GRCGYC 1 cut(s) 1387
Bst2UI CCWGG 2 cut(s) 636, 1736
Bst4CI ACNGT 8 cut(s) 123, 146, 232, 822, 1099, 1162, 1409, 1456
Bst6I CTCTTC 2 cut(s) 488, 1851
BstACI GRCGYC 1 cut(s) 1387
BstAPI GCANNNNNTGC 1 cut(s) 1077
BstBAI YACGTR 1 cut(s) 1619
BstBI TTCGAA 1 cut(s) 414
BstC8I GCNNGC 5 cut(s) 966, 1069, 1794, 1819, 1823
BstDEI CTNAG 4 cut(s) 219, 351, 534, 891
BstDSI CCRYGG 2 cut(s) 705, 1190
BstF5I GGATG 4 cut(s) 40, 758, 1071, 1942
BstFNI CGCG 3 cut(s) 707, 1608, 1679
BstHHI GCGC 1 cut(s) 1679
BstKTI GATC 7 cut(s) 151, 225, 313, 385, 804, 1045, 1836
BstMAI GTCTC 7 cut(s) 167, 610, 1675, 1676, 1748, 1754, 1893
BstMBI GATC 7 cut(s) 148, 222, 310, 382, 801, 1042, 1833
BstMCI CGRYCG 2 cut(s) 1520, 1836
BstMWI GCNNNNNNNGC 8 cut(s) 37, 106, 918, 1077, 1118, 1685, 1789, 1883
BstNI CCWGG 2 cut(s) 636, 1736
BstSCI CCNGG 2 cut(s) 634, 1734
BstSFI CTRYAG 2 cut(s) 1474, 1713
BstUI CGCG 3 cut(s) 707, 1608, 1679
BstV1I GCAGC 5 cut(s) 1000, 1319, 1541, 1826, 1870
BstV2I GAAGAC 1 cut(s) 268
BstX2I RGATCY 1 cut(s) 222
BstXI CCANNNNNNTGG 1 cut(s) 1409
BstYI RGATCY 1 cut(s) 222
BsuRI GGCC 4 cut(s) 1121, 1172, 1345, 1550
BtgI CCRYGG 2 cut(s) 705, 1190
BtsCI GGATG 4 cut(s) 40, 758, 1071, 1942
BtsI GCAGTG 1 cut(s) 1551
BtsIMutI CAGTG 5 cut(s) 335, 1395, 1443, 1461, 1551
Cac8I GCNNGC 5 cut(s) 966, 1069, 1794, 1819, 1823
CciI TCATGA 1 cut(s) 832
CfoI GCGC 1 cut(s) 1679
Cfr10I RCCGGY 1 cut(s) 1519
Cfr13I GGNCC 4 cut(s) 9, 14, 123, 332
Cfr42I CCGCGG 1 cut(s) 708
CpoI CGGWCCG 1 cut(s) 9
CseI GACGC 2 cut(s) 1376, 1875
Csp6I GTAC 4 cut(s) 658, 710, 1405, 1918
CspAI ACCGGT 1 cut(s) 1519
CspCI CAANNNNNGTGG 2 cut(s) 1179, 1214
CspI CGGWCCG 1 cut(s) 9
CviAII CATG 4 cut(s) 380, 467, 833, 1597
CviQI GTAC 4 cut(s) 658, 710, 1405, 1918
DdeI CTNAG 4 cut(s) 219, 351, 534, 891
DpnI GATC 7 cut(s) 150, 224, 312, 384, 803, 1044, 1835
DpnII GATC 7 cut(s) 148, 222, 310, 382, 801, 1042, 1833
DrdI GACNNNNNNGTC 1 cut(s) 689
DriI GACNNNNNGTC 1 cut(s) 121
DseDI GACNNNNNNGTC 1 cut(s) 689
EaeI YGGCCR 2 cut(s) 1170, 1548
Eam1104I CTCTTC 2 cut(s) 488, 1851
Eam1105I GACNNNNNGTC 1 cut(s) 121
EarI CTCTTC 2 cut(s) 488, 1851
EciI GGCGGA 1 cut(s) 628
Eco47I GGWCC 4 cut(s) 9, 14, 123, 332
Eco57I CTGAAG 2 cut(s) 212, 866
Eco88I CYCGRG 1 cut(s) 1878
EcoRII CCWGG 2 cut(s) 634, 1734
Esp3I CGTCTC 2 cut(s) 1754, 1893
FaeI CATG 4 cut(s) 383, 470, 836, 1600
FaqI GGGAC 2 cut(s) 594, 687
FatI CATG 4 cut(s) 379, 466, 832, 1596
FauI CCCGC 1 cut(s) 712
FbaI TGATCA 1 cut(s) 1042
FblI GTMKAC 1 cut(s) 1516
Fnu4HI GCNGC 5 cut(s) 989, 1308, 1530, 1815, 1884
FokI GGATG 4 cut(s) 27, 765, 1058, 1929
Fsp4HI GCNGC 5 cut(s) 989, 1308, 1530, 1815, 1884
FspBI CTAG 5 cut(s) 45, 159, 242, 965, 1068
GlaI GCGC 1 cut(s) 1678
GluI GCNGC 5 cut(s) 989, 1308, 1530, 1815, 1884
GsuI CTGGAG 2 cut(s) 149, 657
HaeIII GGCC 4 cut(s) 1121, 1172, 1345, 1550
HapII CCGG 2 cut(s) 12, 1520
HgaI GACGC 2 cut(s) 1376, 1875
HhaI GCGC 1 cut(s) 1679
Hin1I GRCGYC 1 cut(s) 1387
Hin1II CATG 4 cut(s) 383, 470, 836, 1600
Hin6I GCGC 1 cut(s) 1677
HinP1I GCGC 1 cut(s) 1677
HincII GTYRAC 2 cut(s) 1149, 1517
HindII GTYRAC 2 cut(s) 1149, 1517
HindIII AAGCTT 2 cut(s) 587, 1741
HinfI GANTC 9 cut(s) 187, 264, 683, 931, 1090, 1201, 1429, 1769, 1909
HpaII CCGG 2 cut(s) 12, 1520
HphI GGTGA 1 cut(s) 1865
Hpy166II GTNNAC 5 cut(s) 335, 1149, 1333, 1494, 1517
Hpy188I TCNGA 6 cut(s) 186, 192, 222, 310, 1095, 1579
Hpy188III TCNNGA 8 cut(s) 166, 445, 833, 1128, 1205, 1364, 1466, 1773
Hpy8I GTNNAC 5 cut(s) 335, 1149, 1333, 1494, 1517
HpyAV CCTTC 6 cut(s) 535, 871, 1213, 1793, 1836, 1919
HpyCH4III ACNGT 8 cut(s) 123, 146, 232, 822, 1099, 1162, 1409, 1456
HpyCH4IV ACGT 2 cut(s) 238, 1618
HpyF10VI GCNNNNNNNGC 8 cut(s) 37, 106, 918, 1077, 1118, 1685, 1789, 1883
HpyF3I CTNAG 4 cut(s) 219, 351, 534, 891
HpySE526I ACGT 2 cut(s) 238, 1618
Hsp92I GRCGYC 1 cut(s) 1387
Hsp92II CATG 4 cut(s) 383, 470, 836, 1600
HspAI GCGC 1 cut(s) 1677
KpnI GGTACC 1 cut(s) 713
Ksp22I TGATCA 1 cut(s) 1042
KspI CCGCGG 1 cut(s) 708
Kzo9I GATC 7 cut(s) 148, 222, 310, 382, 801, 1042, 1833
LmnI GCTCC 3 cut(s) 361, 724, 1064
Lsp1109I GCAGC 5 cut(s) 1000, 1319, 1541, 1826, 1870
LweI GCATC 6 cut(s) 49, 572, 1080, 1345, 1893, 1951
MaeI CTAG 5 cut(s) 45, 159, 242, 965, 1068
MaeII ACGT 2 cut(s) 238, 1618
MaeIII GTNAC 4 cut(s) 3, 372, 1178, 1393
MalI GATC 7 cut(s) 150, 224, 312, 384, 803, 1044, 1835
MboI GATC 7 cut(s) 148, 222, 310, 382, 801, 1042, 1833
MfeI CAATTG 2 cut(s) 1275, 1689
MflI RGATCY 1 cut(s) 222
MhlI GDGCHC 1 cut(s) 1189
MlsI TGGCCA 2 cut(s) 1172, 1550
MluNI TGGCCA 2 cut(s) 1172, 1550
MlyI GAGTC 5 cut(s) 196, 258, 677, 1423, 1903
Mox20I TGGCCA 2 cut(s) 1172, 1550
MroXI GAANNNNTTC 2 cut(s) 1329, 1929
MscI TGGCCA 2 cut(s) 1172, 1550
MseI TTAA 4 cut(s) 663, 954, 1778, 1895
MslI CAYNNNNRTG 1 cut(s) 831
Msp20I TGGCCA 2 cut(s) 1172, 1550
MspA1I CMGCKG 3 cut(s) 707, 1019, 1817
MspI CCGG 2 cut(s) 12, 1520
MspR9I CCNGG 2 cut(s) 636, 1736
MunI CAATTG 2 cut(s) 1275, 1689
Mva1269I GAATGC 2 cut(s) 410, 1218
MvaI CCWGG 2 cut(s) 636, 1736
MvnI CGCG 3 cut(s) 707, 1608, 1679
MwoI GCNNNNNNNGC 8 cut(s) 37, 106, 918, 1077, 1118, 1685, 1789, 1883
NdeII GATC 7 cut(s) 148, 222, 310, 382, 801, 1042, 1833
NheI GCTAGC 2 cut(s) 964, 1067
NlaIII CATG 4 cut(s) 383, 470, 836, 1600
NlaIV GGNNCC 1 cut(s) 711
NmeAIII GCCGAG 1 cut(s) 1325
NmuCI GTSAC 2 cut(s) 3, 1393
NspV TTCGAA 1 cut(s) 414
PaeR7I CTCGAG 1 cut(s) 1878
PagI TCATGA 1 cut(s) 832
PcsI WCGNNNNNNNCGW 1 cut(s) 1829
PctI GAATGC 2 cut(s) 410, 1218
PdmI GAANNNNTTC 2 cut(s) 1329, 1929
PfeI GAWTC 4 cut(s) 931, 1090, 1201, 1769
PinAI ACCGGT 1 cut(s) 1519
PkrI GCNGC 5 cut(s) 990, 1309, 1531, 1816, 1885
Ple19I CGATCG 1 cut(s) 1836
PleI GAGTC 5 cut(s) 195, 258, 677, 1423, 1903
PpsI GAGTC 5 cut(s) 195, 258, 677, 1423, 1903
Ppu21I YACGTR 1 cut(s) 1619
PsiI TTATAA 2 cut(s) 984, 1628
Psp6I CCWGG 2 cut(s) 634, 1734
PspGI CCWGG 2 cut(s) 634, 1734
PspN4I GGNNCC 1 cut(s) 711
PspPI GGNCC 4 cut(s) 9, 14, 123, 332
PspXI VCTCGAGB 1 cut(s) 1878
PstI CTGCAG 1 cut(s) 1717
PsuI RGATCY 1 cut(s) 222
PvuI CGATCG 1 cut(s) 1836
PvuII CAGCTG 2 cut(s) 1019, 1817
RsaI GTAC 4 cut(s) 659, 711, 1406, 1919
RsaNI GTAC 4 cut(s) 658, 710, 1405, 1918
RseI CAYNNNNRTG 1 cut(s) 831
Rsr2I CGGWCCG 1 cut(s) 9
RsrII CGGWCCG 1 cut(s) 9
SacII CCGCGG 1 cut(s) 708
SalI GTCGAC 1 cut(s) 1515
SaqAI TTAA 4 cut(s) 663, 954, 1778, 1895
SatI GCNGC 5 cut(s) 989, 1308, 1530, 1815, 1884
Sau3AI GATC 7 cut(s) 148, 222, 310, 382, 801, 1042, 1833
Sau96I GGNCC 4 cut(s) 9, 14, 123, 332
SchI GAGTC 5 cut(s) 196, 258, 677, 1423, 1903
ScrFI CCNGG 2 cut(s) 636, 1736
SduI GDGCHC 1 cut(s) 1189
SfaNI GCATC 6 cut(s) 49, 572, 1080, 1345, 1893, 1951
SfcI CTRYAG 2 cut(s) 1474, 1713
Sfr274I CTCGAG 1 cut(s) 1878
Sfr303I CCGCGG 1 cut(s) 708
SfuI TTCGAA 1 cut(s) 414
SgrBI CCGCGG 1 cut(s) 708
SinI GGWCC 4 cut(s) 9, 14, 123, 332
SlaI CTCGAG 1 cut(s) 1878
SmiMI CAYNNNNRTG 1 cut(s) 831
SmlI CTYRAG 2 cut(s) 1672, 1878
SmoI CTYRAG 2 cut(s) 1672, 1878
SsiI CCGC 5 cut(s) 613, 705, 707, 1297, 1679
SspMI CTAG 5 cut(s) 45, 159, 242, 965, 1068
StyD4I CCNGG 2 cut(s) 634, 1734
TaaI ACNGT 8 cut(s) 123, 146, 232, 822, 1099, 1162, 1409, 1456
TaiI ACGT 2 cut(s) 241, 1621
TaqI TCGA 9 cut(s) 414, 444, 686, 917, 1052, 1500, 1516, 1832, 1879
TfiI GAWTC 4 cut(s) 931, 1090, 1201, 1769
Tru1I TTAA 4 cut(s) 663, 954, 1778, 1895
Tru9I TTAA 4 cut(s) 663, 954, 1778, 1895
TscAI CASTG 5 cut(s) 342, 1402, 1443, 1461, 1551
TseFI GTSAC 2 cut(s) 3, 1393
TseI GCWGC 5 cut(s) 988, 1307, 1529, 1814, 1883
Tsp45I GTSAC 2 cut(s) 3, 1393
TspDTI ATGAA 6 cut(s) 638, 821, 870, 944, 960, 1016
TspGWI ACGGA 5 cut(s) 22, 101, 1224, 1778, 1902
TspRI CASTG 5 cut(s) 342, 1402, 1443, 1461, 1551
VpaK11BI GGWCC 4 cut(s) 9, 14, 123, 332
XapI RAATTY 4 cut(s) 418, 977, 1315, 1370
XcmI CCANNNNNNNNNTGG 1 cut(s) 24
XhoI CTCGAG 1 cut(s) 1878
XmiI GTMKAC 1 cut(s) 1516
XmnI GAANNNNTTC 2 cut(s) 1329, 1929
XspI CTAG 5 cut(s) 45, 159, 242, 965, 1068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.