MD08G1148100.v1.1

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0007203
Physical Location & Seq
Reverse (-)
14900032 .. 14904991
4960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1148100.v1.1.491

Sequence Viewer

Length: 1836 bp
ATGAGAAAAGCGTCGTCGATCTCAGCGCGTTTGTTTCTAGGGTTTGTTCGAGGCCAAACAACCTCCAAGCTCCGGTGCTTCAATGCGGTGCGTTTTCACTGTGATCGAGCCCAACCTTCCTCTATTAAGCCTCCAGGTCCAGGGCCACTAACGCAGTACAAGAGCCTCGTCGAGGAAGGGAAGCTGCAGCATGATCCGAAGCAAGAGACGGTGGCGTTTCAATTGGAGGAGTTGCTCGGGAGATTGGAGCAGTACGAGAGAGAAATGGAGGATTATCATGAAAAGCTTGCGATTTGGGAGAGGCGGAGAGAGGACGAGCGGCGGAAGCTTTTAATGGAGGAAGCTGAGAGCCAGCAGAAAGATGATGTATGGACATCAGTCACCAACCAGCGCAATAAGTTTGTGCAGAGATGGCTGTCCCGGAAGAAACCTGATAAAGCAGAACCCGGAGTGGGGAAATGGGTTTCGTATCTTAATCGAGAGAGGAAGTTAGATTCACGCGTTGGTCGCCGCCCAACTGCTCCTCCGGCTCCCAAGGGACTGTATATTTACGGCAATGTTGGAAGTGGAAAGACGATGCTTATGGACATGTTTTTTAGTGCCACTGAAGGAATAGTTAAGCATCGAAAGAGATATCACTTTCACGAGGCAATGTTAAAGATTAACGAACAAATGCATCAGATTTGGAAAAGCCAAGTGGAGGAAAAGTCTTTGCATTCAAGCCTTTCCAATTGGGTAATGAATCTTCCCTTTGATTCGAGGGTGAAGGAGTGGGTCGCTGCAGAAGAGAGATATAAGCAAGAGGTTCAGATGAAAAACATCCTTCCAGCTGTAGCAGATAAGCTGCTCATAGACCGGCAGGCAAATCAAAAGGGTGGTAGCATTCTCTGTTTTGACGAAATACAGACTGTTGATGTATTTGCTATTGTGGCCTTATCTGGAATTGTTAGCAGATTGTTAAGTACCGGAACTGTTTTTGTTGCCACCAGTAATCGAGCGCCAACAGACTTAAATCAGGATGGTATGCAGCGGGAGATCTTCCAAAAGTTTGTTCGCAAACTGAATGAGCACTGTGAGACTGTACTCATTGGAAGTGAGATTGATTATCGTCGTGTTATAGCTCAAAGTCAAAGATCTGCAGACCAGGTTCACTACTTCTGGCCCTCAGACAGCGTTGCTATGGAGAAATTTGAGAAGAAGTGGCATGAAGTCACAAACCAAATAGGGGGACAGATTGCCTCGGATACTATTCGAGTGATGTTCGGGAGAACACTGGAGGTTCCCGAATGCTCTAATGGAGCGGCACGGTTCACATTTGATTATCTCTGTGGTCGGCCGGTAGGTGCAGCTGATTATATTGCAGTTGCAGAAAATTATCACACTATATTTATATCTGACATTCCAATGATGAGTATGCGTATCCGTGATAAGGCACGGCGGTTTATTACCCTCATTGATGAATTATACAATCATCATTGCCGTCTATTTTGTTCTGCAGCATCTTCTATTGATGAATTATTTCAAGGAACTGAAGAGGGTACTCTTTTTGATCTGGAGAGTTTCCAGTTTGAAACAGAGACAGAAGGTTCAAAGCTTCGTCGCGATGTTTTAGCAGAAGGGAATGTTAGTTCAGGAGGTGCCCCAACTGGTATAATATCCATGCTATCTGGTCAAGAAGAGATGTTTGCTTTCCGCAGAGCAGTGTCGCGCTTGATTGAAATGCAGACGCCCGTATATTTGGAGGGAGTCCACAGTCTTCATCCGTATTTCCAGAAACAACGCCGGGGATTTGAAAGTATATCTTCACGAAACATACAGCCGCAGTTGTCATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

612

Amino Acids

70.33

Weight (kDa)

8.57

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 163 - 511 3.4e-61 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1637
AccBSI CCGCTC 2 cut(s) 319, 1301
AccII CGCG 4 cut(s) 28, 501, 1602, 1708
AclWI GGATC 1 cut(s) 188
AcoI YGGCCR 1 cut(s) 1334
AcsI RAATTY 1 cut(s) 1187
AcuI CTGAAG 2 cut(s) 627, 1551
AcyI GRCGYC 1 cut(s) 1727
AfaI GTAC 5 cut(s) 158, 254, 964, 1083, 1540
AfiI CCNNNNNNNGG 8 cut(s) 72, 140, 172, 452, 453, 700, 1225, 1429
AflIII ACRYGT 2 cut(s) 499, 588
AgsI TTSAA 8 cut(s) 82, 221, 720, 1523, 1571, 1590, 1718, 1793
AjnI CCWGG 3 cut(s) 133, 139, 1143
AjuI GAANNNNNNNTTGG 2 cut(s) 1035, 1067
AloI GAACNNNNNNTCC 2 cut(s) 1612, 1644
Alw21I GWGCWC 1 cut(s) 1071
Alw26I GTCTC 3 cut(s) 200, 1070, 1571
AlwI GGATC 1 cut(s) 188
Ama87I CYCGRG 1 cut(s) 236
AoxI GGCC 5 cut(s) 52, 143, 930, 1160, 1334
ApeKI GCWGC 7 cut(s) 184, 187, 779, 844, 1027, 1346, 1496
ApoI RAATTY 1 cut(s) 1187
Asp700I GAANNNNTTC 1 cut(s) 1518
AspLEI GCGC 4 cut(s) 28, 393, 1000, 1710
AspS9I GGNCC 3 cut(s) 137, 143, 1161
AsuC2I CCSGG 3 cut(s) 421, 447, 1784
AsuHPI GGTGA 2 cut(s) 373, 775
AvaI CYCGRG 1 cut(s) 236
AvaII GGWCC 1 cut(s) 137
BaeGI GKGCMC 1 cut(s) 1642
BanI GGYRCC 1 cut(s) 1637
BanII GRGCYC 1 cut(s) 112
BarI GAAGNNNNNNTAC 2 cut(s) 729, 761
BauI CACGAG 1 cut(s) 644
BbsI GAAGAC 1 cut(s) 1748
Bbv12I GWGCWC 1 cut(s) 1071
BbvI GCAGC 7 cut(s) 171, 199, 766, 831, 1039, 1358, 1508
BccI CCATC 2 cut(s) 405, 1013
BceAI ACGGC 3 cut(s) 568, 1451, 1464
BciT130I CCWGG 3 cut(s) 135, 141, 1145
BciVI GTATCC 2 cut(s) 1237, 1430
BcnI CCSGG 3 cut(s) 421, 447, 1784
BcoDI GTCTC 3 cut(s) 200, 1070, 1571
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 5 cut(s) 185, 780, 831, 1137, 1494
BfoI RGCGCY 1 cut(s) 1001
BfuI GTATCC 2 cut(s) 1237, 1430
BglII AGATCT 2 cut(s) 1035, 1133
Bme1390I CCNGG 6 cut(s) 135, 141, 421, 447, 1145, 1784
Bme18I GGWCC 1 cut(s) 137
BmeT110I CYCGRG 1 cut(s) 236
BmgT120I GGNCC 3 cut(s) 137, 143, 1161
BmiI GGNNCC 3 cut(s) 531, 1281, 1639
BmrFI CCNGG 6 cut(s) 135, 141, 421, 447, 1145, 1784
BmsI GCATC 4 cut(s) 567, 631, 685, 1508
BoxI GACNNNNGTC 1 cut(s) 377
BpiI GAAGAC 1 cut(s) 1748
BpmI CTGGAG 3 cut(s) 117, 1295, 1574
BpuMI CCSGG 3 cut(s) 421, 447, 1784
BsaHI GRCGYC 1 cut(s) 1727
BsaJI CCNNGG 4 cut(s) 140, 534, 1239, 1783
BsaWI WCCGGW 2 cut(s) 72, 965
BsaXI ACNNNNNCTCC 4 cut(s) 508, 538, 692, 722
Bsc4I CCNNNNNNNGG 8 cut(s) 72, 140, 172, 452, 453, 700, 1225, 1429
Bse118I RCCGGY 2 cut(s) 855, 1336
Bse1I ACTGG 4 cut(s) 987, 1278, 1564, 1651
Bse3DI GCAATG 3 cut(s) 562, 657, 1474
BseBI CCWGG 3 cut(s) 135, 141, 1145
BseDI CCNNGG 4 cut(s) 140, 534, 1239, 1783
BseGI GGATG 3 cut(s) 819, 1024, 1759
BseLI CCNNNNNNNGG 8 cut(s) 72, 140, 172, 452, 453, 700, 1225, 1429
BseMI GCAATG 3 cut(s) 562, 657, 1474
BseMII CTCAG 3 cut(s) 36, 336, 1179
BseNI ACTGG 4 cut(s) 987, 1278, 1564, 1651
BseRI GAGGAG 2 cut(s) 242, 513
BseSI GKGCMC 1 cut(s) 1642
BseX3I CGGCCG 1 cut(s) 1334
BseXI GCAGC 7 cut(s) 171, 199, 766, 831, 1039, 1358, 1508
BsgI GTGCAG 2 cut(s) 425, 1365
Bsh1236I CGCG 4 cut(s) 28, 501, 1602, 1708
Bsh1285I CGRYCG 1 cut(s) 1337
BshFI GGCC 5 cut(s) 54, 145, 932, 1162, 1336
BshNI GGYRCC 1 cut(s) 1637
BsiEI CGRYCG 1 cut(s) 1337
BsiHKAI GWGCWC 1 cut(s) 1071
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 8 cut(s) 73, 421, 447, 527, 856, 966, 1337, 1783
BslFI GGGAC 3 cut(s) 403, 552, 1242
BslI CCNNNNNNNGG 8 cut(s) 72, 140, 172, 452, 453, 700, 1225, 1429
BsmAI GTCTC 3 cut(s) 200, 1070, 1571
BsmBI CGTCTC 1 cut(s) 200
BsmFI GGGAC 3 cut(s) 403, 552, 1242
BsmI GAATGC 3 cut(s) 715, 882, 1292
BsnI GGCC 5 cut(s) 54, 145, 932, 1162, 1336
BsoBI CYCGRG 1 cut(s) 236
Bsp1286I GDGCHC 3 cut(s) 112, 1071, 1642
Bsp143I GATC 6 cut(s) 18, 103, 193, 1035, 1133, 1549
Bsp68I TCGCGA 1 cut(s) 1602
BspANI GGCC 5 cut(s) 54, 145, 932, 1162, 1336
BspCNI CTCAG 3 cut(s) 35, 337, 1178
BspFNI CGCG 4 cut(s) 28, 501, 1602, 1708
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 3 cut(s) 531, 1281, 1639
BspMAI CTGCAG 4 cut(s) 189, 784, 1141, 1498
BspPI GGATC 1 cut(s) 188
BspT107I GGYRCC 1 cut(s) 1637
BsrBI CCGCTC 2 cut(s) 319, 1301
BsrDI GCAATG 3 cut(s) 562, 657, 1474
BsrFI RCCGGY 2 cut(s) 855, 1336
BsrI ACTGG 4 cut(s) 987, 1278, 1564, 1651
BssAI RCCGGY 2 cut(s) 855, 1336
BssECI CCNNGG 4 cut(s) 140, 534, 1239, 1783
BssMI GATC 6 cut(s) 18, 103, 193, 1035, 1133, 1549
BssNI GRCGYC 1 cut(s) 1727
BssSI CACGAG 1 cut(s) 644
BssT1I CCWWGG 1 cut(s) 534
Bst2BI CACGAG 1 cut(s) 644
Bst2UI CCWGG 3 cut(s) 135, 141, 1145
Bst4CI ACNGT 9 cut(s) 101, 211, 543, 910, 973, 1073, 1081, 1308, 1754
Bst6I CTCTTC 3 cut(s) 780, 1527, 1671
BstACI GRCGYC 1 cut(s) 1727
BstC8I GCNNGC 3 cut(s) 288, 353, 861
BstDEI CTNAG 3 cut(s) 22, 345, 1165
BstENI CCTNNNNNAGG 1 cut(s) 170
BstF5I GGATG 3 cut(s) 819, 1024, 1759
BstFNI CGCG 4 cut(s) 28, 501, 1602, 1708
BstH2I RGCGCY 1 cut(s) 1001
BstHHI GCGC 4 cut(s) 28, 393, 1000, 1710
BstKTI GATC 6 cut(s) 21, 106, 196, 1038, 1136, 1552
BstMAI GTCTC 3 cut(s) 200, 1070, 1571
BstMBI GATC 6 cut(s) 18, 103, 193, 1035, 1133, 1549
BstMCI CGRYCG 1 cut(s) 1337
BstMWI GCNNNNNNNGC 6 cut(s) 151, 325, 412, 507, 527, 929
BstNI CCWGG 3 cut(s) 135, 141, 1145
BstNSI RCATGY 1 cut(s) 592
BstPAI GACNNNNGTC 1 cut(s) 377
BstSCI CCNGG 6 cut(s) 133, 139, 419, 445, 1143, 1782
BstSFI CTRYAG 5 cut(s) 185, 780, 831, 1137, 1494
BstSLI GKGCMC 1 cut(s) 1642
BstUI CGCG 4 cut(s) 28, 501, 1602, 1708
BstV1I GCAGC 7 cut(s) 171, 199, 766, 831, 1039, 1358, 1508
BstV2I GAAGAC 1 cut(s) 1748
BstX2I RGATCY 2 cut(s) 1035, 1133
BstYI RGATCY 2 cut(s) 1035, 1133
BstZI CGGCCG 1 cut(s) 1334
BsuI GTATCC 2 cut(s) 1237, 1430
BsuRI GGCC 5 cut(s) 54, 145, 932, 1162, 1336
BtgZI GCGATG 1 cut(s) 1617
BtsCI GGATG 3 cut(s) 819, 1024, 1759
BtsI GCAGTG 1 cut(s) 1707
BtsIMutI CAGTG 5 cut(s) 97, 603, 1069, 1271, 1707
BtuMI TCGCGA 1 cut(s) 1602
Cac8I GCNNGC 3 cut(s) 288, 353, 861
CciI TCATGA 1 cut(s) 277
CfoI GCGC 4 cut(s) 28, 393, 1000, 1710
Cfr10I RCCGGY 2 cut(s) 855, 1336
Cfr13I GGNCC 3 cut(s) 137, 143, 1161
CseI GACGC 1 cut(s) 1735
CsiI ACCWGGT 1 cut(s) 1143
Csp6I GTAC 5 cut(s) 157, 253, 963, 1082, 1539
CviAII CATG 5 cut(s) 191, 278, 589, 1205, 1660
CviQI GTAC 5 cut(s) 157, 253, 963, 1082, 1539
DdeI CTNAG 3 cut(s) 22, 345, 1165
DpnI GATC 6 cut(s) 20, 105, 195, 1037, 1135, 1551
DpnII GATC 6 cut(s) 18, 103, 193, 1035, 1133, 1549
EaeI YGGCCR 1 cut(s) 1334
EagI CGGCCG 1 cut(s) 1334
Eam1104I CTCTTC 3 cut(s) 780, 1527, 1671
EarI CTCTTC 3 cut(s) 780, 1527, 1671
EciI GGCGGA 2 cut(s) 319, 337
EclXI CGGCCG 1 cut(s) 1334
Eco130I CCWWGG 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 112
Eco32I GATATC 1 cut(s) 635
Eco47I GGWCC 1 cut(s) 137
Eco52I CGGCCG 1 cut(s) 1334
Eco57I CTGAAG 2 cut(s) 627, 1551
Eco88I CYCGRG 1 cut(s) 236
EcoNI CCTNNNNNAGG 1 cut(s) 170
EcoRII CCWGG 3 cut(s) 133, 139, 1143
EcoRV GATATC 1 cut(s) 635
EcoT14I CCWWGG 1 cut(s) 534
EcoT22I ATGCAT 1 cut(s) 678
EcoT38I GRGCYC 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 534
Esp3I CGTCTC 1 cut(s) 200
FaeI CATG 5 cut(s) 194, 281, 592, 1208, 1663
FalI AAGNNNNNCTT 2 cut(s) 1786, 1818
FaqI GGGAC 3 cut(s) 403, 552, 1242
FatI CATG 5 cut(s) 190, 277, 588, 1204, 1659
FauI CCCGC 1 cut(s) 1023
FokI GGATG 3 cut(s) 806, 1031, 1746
FriOI GRGCYC 1 cut(s) 112
FspBI CTAG 1 cut(s) 38
GlaI GCGC 4 cut(s) 27, 392, 999, 1709
GsuI CTGGAG 3 cut(s) 117, 1295, 1574
HaeII RGCGCY 1 cut(s) 1001
HaeIII GGCC 5 cut(s) 54, 145, 932, 1162, 1336
HapII CCGG 8 cut(s) 73, 421, 447, 527, 856, 966, 1337, 1783
HgaI GACGC 1 cut(s) 1735
HhaI GCGC 4 cut(s) 28, 393, 1000, 1710
Hin1I GRCGYC 1 cut(s) 1727
Hin1II CATG 5 cut(s) 194, 281, 592, 1208, 1663
Hin6I GCGC 4 cut(s) 26, 391, 998, 1708
HinP1I GCGC 4 cut(s) 26, 391, 998, 1708
HindIII AAGCTT 3 cut(s) 284, 326, 1592
HinfI GANTC 4 cut(s) 494, 742, 755, 1746
HpaII CCGG 8 cut(s) 73, 421, 447, 527, 856, 966, 1337, 1783
HphI GGTGA 2 cut(s) 373, 775
Hpy166II GTNNAC 3 cut(s) 1150, 1311, 1750
Hpy188I TCNGA 6 cut(s) 198, 681, 810, 1168, 1243, 1396
Hpy8I GTNNAC 3 cut(s) 1150, 1311, 1750
Hpy99I CGWCG 5 cut(s) 16, 19, 173, 1113, 1602
HpyAV CCTTC 7 cut(s) 126, 170, 602, 760, 833, 1577, 1610
HpyCH4III ACNGT 9 cut(s) 101, 211, 543, 910, 973, 1073, 1081, 1308, 1754
HpyF10VI GCNNNNNNNGC 6 cut(s) 151, 325, 412, 507, 527, 929
HpyF3I CTNAG 3 cut(s) 22, 345, 1165
Hsp92I GRCGYC 1 cut(s) 1727
Hsp92II CATG 5 cut(s) 194, 281, 592, 1208, 1663
HspAI GCGC 4 cut(s) 26, 391, 998, 1708
Kzo9I GATC 6 cut(s) 18, 103, 193, 1035, 1133, 1549
LmnI GCTCC 5 cut(s) 75, 247, 526, 535, 1298
Lsp1109I GCAGC 7 cut(s) 171, 199, 766, 831, 1039, 1358, 1508
LweI GCATC 4 cut(s) 567, 631, 685, 1508
MabI ACCWGGT 1 cut(s) 1143
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 2 cut(s) 379, 1210
MalI GATC 6 cut(s) 20, 105, 195, 1037, 1135, 1551
MbiI CCGCTC 2 cut(s) 319, 1301
MboI GATC 6 cut(s) 18, 103, 193, 1035, 1133, 1549
MfeI CAATTG 2 cut(s) 221, 730
MflI RGATCY 2 cut(s) 1035, 1133
MhlI GDGCHC 3 cut(s) 112, 1071, 1642
MluCI AATT 7 cut(s) 221, 730, 942, 1187, 1372, 1460, 1514
MluI ACGCGT 1 cut(s) 499
MlyI GAGTC 1 cut(s) 1755
MmeI TCCRAC 1 cut(s) 541
Mph1103I ATGCAT 1 cut(s) 678
MroXI GAANNNNTTC 1 cut(s) 1518
MseI TTAA 9 cut(s) 126, 332, 474, 618, 656, 663, 959, 1010, 1834
MslI CAYNNNNRTG 1 cut(s) 1403
MspA1I CMGCKG 3 cut(s) 830, 1030, 1349
MspI CCGG 8 cut(s) 73, 421, 447, 527, 856, 966, 1337, 1783
MspR9I CCNGG 6 cut(s) 135, 141, 421, 447, 1145, 1784
MunI CAATTG 2 cut(s) 221, 730
Mva1269I GAATGC 3 cut(s) 715, 882, 1292
MvaI CCWGG 3 cut(s) 135, 141, 1145
MvnI CGCG 4 cut(s) 28, 501, 1602, 1708
MwoI GCNNNNNNNGC 6 cut(s) 151, 325, 412, 507, 527, 929
NciI CCSGG 3 cut(s) 421, 447, 1784
NdeII GATC 6 cut(s) 18, 103, 193, 1035, 1133, 1549
NlaIII CATG 5 cut(s) 194, 281, 592, 1208, 1663
NlaIV GGNNCC 3 cut(s) 531, 1281, 1639
NmuCI GTSAC 2 cut(s) 379, 1210
NruI TCGCGA 1 cut(s) 1602
NsiI ATGCAT 1 cut(s) 678
NspI RCATGY 1 cut(s) 592
PagI TCATGA 1 cut(s) 277
PciI ACATGT 1 cut(s) 588
PctI GAATGC 3 cut(s) 715, 882, 1292
PdmI GAANNNNTTC 1 cut(s) 1518
PfeI GAWTC 3 cut(s) 494, 742, 755
PfoI TCCNGGA 1 cut(s) 419
PleI GAGTC 1 cut(s) 1754
PpsI GAGTC 1 cut(s) 1754
PscI ACATGT 1 cut(s) 588
PshAI GACNNNNGTC 1 cut(s) 377
Psp6I CCWGG 3 cut(s) 133, 139, 1143
PspGI CCWGG 3 cut(s) 133, 139, 1143
PspN4I GGNNCC 3 cut(s) 531, 1281, 1639
PspPI GGNCC 3 cut(s) 137, 143, 1161
PstI CTGCAG 4 cut(s) 189, 784, 1141, 1498
PsuI RGATCY 2 cut(s) 1035, 1133
PvuII CAGCTG 2 cut(s) 830, 1349
RruI TCGCGA 1 cut(s) 1602
RsaI GTAC 5 cut(s) 158, 254, 964, 1083, 1540
RsaNI GTAC 5 cut(s) 157, 253, 963, 1082, 1539
RseI CAYNNNNRTG 1 cut(s) 1403
SaqAI TTAA 9 cut(s) 126, 332, 474, 618, 656, 663, 959, 1010, 1834
Sau3AI GATC 6 cut(s) 18, 103, 193, 1035, 1133, 1549
Sau96I GGNCC 3 cut(s) 137, 143, 1161
SchI GAGTC 1 cut(s) 1755
ScrFI CCNGG 6 cut(s) 135, 141, 421, 447, 1145, 1784
SduI GDGCHC 3 cut(s) 112, 1071, 1642
SexAI ACCWGGT 1 cut(s) 1143
SfaNI GCATC 4 cut(s) 567, 631, 685, 1508
SfcI CTRYAG 5 cut(s) 185, 780, 831, 1137, 1494
SinI GGWCC 1 cut(s) 137
SmiMI CAYNNNNRTG 1 cut(s) 1403
Sse9I AATT 7 cut(s) 221, 730, 942, 1187, 1372, 1460, 1514
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 6 cut(s) 133, 139, 419, 445, 1143, 1782
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 9 cut(s) 101, 211, 543, 910, 973, 1073, 1081, 1308, 1754
TaqI TCGA 9 cut(s) 17, 49, 106, 171, 478, 625, 758, 994, 1252
TasI AATT 7 cut(s) 221, 730, 942, 1187, 1372, 1460, 1514
TatI WGTACW 2 cut(s) 156, 1081
TauI GCSGC 4 cut(s) 322, 513, 1304, 1822
TfiI GAWTC 3 cut(s) 494, 742, 755
Tru1I TTAA 9 cut(s) 126, 332, 474, 618, 656, 663, 959, 1010, 1834
Tru9I TTAA 9 cut(s) 126, 332, 474, 618, 656, 663, 959, 1010, 1834
TscAI CASTG 5 cut(s) 104, 610, 1076, 1278, 1707
TseFI GTSAC 2 cut(s) 379, 1210
TseI GCWGC 7 cut(s) 184, 187, 779, 844, 1027, 1346, 1496
Tsp45I GTSAC 2 cut(s) 379, 1210
TspDTI ATGAA 7 cut(s) 294, 755, 827, 1221, 1473, 1527, 1748
TspGWI ACGGA 2 cut(s) 1412, 1752
TspRI CASTG 5 cut(s) 104, 610, 1076, 1278, 1707
VpaK11BI GGWCC 1 cut(s) 137
XagI CCTNNNNNAGG 1 cut(s) 170
XapI RAATTY 1 cut(s) 1187
XceI RCATGY 1 cut(s) 592
XmnI GAANNNNTTC 1 cut(s) 1518
XspI CTAG 1 cut(s) 38
Zsp2I ATGCAT 1 cut(s) 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.