Rh6BG397900

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0007203
Physical Location & Seq
Forward (+)
62572401 .. 62578761
6361 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG397900.1

Sequence Viewer

Length: 1572 bp
ATGGAGGAATATCATACAAGCCTGGCGAATTGGGAGAAGAAGAGAGAGAATGAGCGGCGGAGGATTTTAATGGAGGAAGCTGAGAGTAAGCAGAAGGATGACATGTGGACGTCAGTGAACAACCAAGGGAATAAGCTTTTCCGGAGATGGATGTCCAAGAAAAGACCTGAGAATGTAGAACCTGGAGTTGGGAAATGGGTTTCGTACTTAAATAGAGAAAGGAAGTTGGATTCAATTGTTGGACGTCGTCCAATTGCTCCTGCAGCTCCGAAAGGACTGTATATTTATGGCAATGTTGGAAGTGGAAAGACGATGCTTATGGACATGTTCTATAGTGCCACTGAAGGAATAGTTAAGCATCGAAAGAGATATCACTTTCATGAGGCCATGTTGAAAATTAATGAACAAATGCATCAGATTTGGAAAAATCAAGTGGCAGATAAGTCTTTGCAGTCCAGCATTGCTAATTGGATAATGAGTCTACCCTTCGATTCGAAGGTCAAGGAGTGGGTGGCTGCAGAAGAGAACTACAAACAACAGTTTCAGATGAAAAACATCCTTCCAGCTGTAGCAGATAAGCTTCTCCTAGATCGGCAGGCAGATCAAAAGGGTGGCAGCATTCTTTGTTTTGATGAGATACAGACTGTTGATGTATTTGCTATTGTGGCCTTATCTGGAATTGTTAGCAGATTGCTAAGTACTGGAACTGTTTTTGTGGCCACCAGTAACCGAGCACCAACAGACTTAAATCAGGATGGTATGCAGAAGGAGATCTTCCAAAAGTTTGTTGCCAAATTGAATGAGCACTGTGAGAATGTTCTGATTGGAAGTGAGATAGATTATCGCCGTGTTATAGCTCAAAATCAAAGATCTAAGGACCAGGATCACTACTTCTGGCCTTCAGACAACATTGCTCTGGAGAAATTTGAGAGAAAGTGGCAAGAAGTCACAAGCCAAGTAGGGGAACAGATCACCTCGAGTACTATTTCGGTGATGTTCGGAAGAAAACTGGAGGTTACTGAAAGCTGTAATGGAGTAGCTCGATTCACATTTGAGTATCTATGTGGTCGGCCTGTAGGTGCAGCAGATTATATTGCAGTAGCAGAAAATTATCATACTGTCTTCATATCTGGCATTCCAGTGATGAGTATGCGTATCCGTGATAAGGCACGGAGGTTTATTACCCTTGTTGATGAGTTATACAATCATCATTGCCGCCTATTTTGTTCTGCAGCTTCTTCTATTGATGATTTGTTTCAAGGAACTGAAGAGGGTGCACTTTTTGATCTAGAGAGTTTCCAGTTTGAAACAGAGACAGAAGGTGCAAAGCTTCGCCGCGATGTTTTAGCAGAAGGGAATGTGAGTTCAGGAGGTGCCCCAACTGGTATAGTATCCATGCTATCTGGTCAAGAAGAAATGTTTGCTTTCCGCCGAGCAGTTTCACGACTGATTGAAATGCAGACACCTGTATACTTGGGGGGAGTTCATAGTCTTCATCCTTATTTCCAGAGACAACGTCAAGAATTTGAAAATATTACTGCAGAAAACTTACAGCCTCAGTTGTCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

523

Amino Acids

59.99

Weight (kDa)

6.67

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 75 - 423 2.1e-64 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 113, 247
AccB1I GGYRCC 1 cut(s) 1373
AccBSI CCGCTC 1 cut(s) 55
AccI GTMKAC 2 cut(s) 481, 1470
AccII CGCG 1 cut(s) 1338
AccIII TCCGGA 1 cut(s) 141
AciI CCGC 5 cut(s) 55, 58, 1216, 1336, 1429
AclWI GGATC 1 cut(s) 891
AcoI YGGCCR 1 cut(s) 717
AcsI RAATTY 2 cut(s) 923, 1523
AcuI CTGAAG 3 cut(s) 363, 885, 1287
AcyI GRCGYC 2 cut(s) 110, 244
AfaI GTAC 3 cut(s) 206, 700, 982
AfiI CCNNNNNNNGG 3 cut(s) 188, 961, 1165
AflIII ACRYGT 2 cut(s) 102, 324
AgsI TTSAA 7 cut(s) 234, 394, 799, 1259, 1307, 1454, 1529
AjnI CCWGG 3 cut(s) 21, 181, 879
AjuI GAANNNNNNNTTGG 2 cut(s) 171, 203
AloI GAACNNNNNNTCC 2 cut(s) 1348, 1380
Alw21I GWGCWC 3 cut(s) 736, 807, 1279
Alw26I GTCTC 2 cut(s) 1307, 1504
Alw44I GTGCAC 1 cut(s) 1275
AlwI GGATC 1 cut(s) 891
Ama87I CYCGRG 1 cut(s) 976
Aor13HI TCCGGA 1 cut(s) 141
AoxI GGCC 5 cut(s) 384, 666, 717, 896, 1070
ApaLI GTGCAC 1 cut(s) 1275
ApeKI GCWGC 5 cut(s) 263, 515, 615, 1082, 1232
ApoI RAATTY 2 cut(s) 923, 1523
AseI ATTAAT 1 cut(s) 399
AspS9I GGNCC 1 cut(s) 877
AsuHPI GGTGA 2 cut(s) 964, 1003
AsuII TTCGAA 1 cut(s) 494
AvaI CYCGRG 1 cut(s) 976
AvaII GGWCC 1 cut(s) 877
BaeGI GKGCMC 2 cut(s) 1279, 1378
BalI TGGCCA 1 cut(s) 719
BanI GGYRCC 1 cut(s) 1373
BbsI GAAGAC 2 cut(s) 1114, 1484
Bbv12I GWGCWC 3 cut(s) 736, 807, 1279
BbvI GCAGC 5 cut(s) 275, 502, 627, 1094, 1244
BccI CCATC 2 cut(s) 141, 749
BceAI ACGGC 1 cut(s) 831
BciT130I CCWGG 3 cut(s) 23, 183, 881
BciVI GTATCC 2 cut(s) 1166, 1402
BcoDI GTCTC 2 cut(s) 1307, 1504
BfaI CTAG 2 cut(s) 587, 1289
BfmI CTRYAG 7 cut(s) 261, 331, 516, 567, 1074, 1230, 1539
BfuI GTATCC 2 cut(s) 1166, 1402
BglII AGATCT 2 cut(s) 771, 869
BisI GCNGC 8 cut(s) 56, 264, 516, 616, 1083, 1216, 1233, 1336
BlsI GCNGC 8 cut(s) 57, 265, 517, 617, 1084, 1217, 1234, 1337
BmcAI AGTACT 2 cut(s) 700, 982
Bme1390I CCNGG 3 cut(s) 23, 183, 881
Bme18I GGWCC 1 cut(s) 877
BmeT110I CYCGRG 1 cut(s) 976
BmgT120I GGNCC 1 cut(s) 877
BmiI GGNNCC 1 cut(s) 1375
BmrFI CCNGG 3 cut(s) 23, 183, 881
BmsI GCATC 3 cut(s) 303, 367, 421
BpiI GAAGAC 2 cut(s) 1114, 1484
BpmI CTGGAG 3 cut(s) 204, 938, 1031
Bpu14I TTCGAA 1 cut(s) 494
BsaHI GRCGYC 2 cut(s) 110, 244
BsaJI CCNNGG 1 cut(s) 124
BsaWI WCCGGW 1 cut(s) 141
Bsc4I CCNNNNNNNGG 3 cut(s) 188, 961, 1165
Bse1I ACTGG 6 cut(s) 706, 723, 1014, 1139, 1300, 1387
Bse3DI GCAATG 4 cut(s) 298, 459, 909, 1210
BseAI TCCGGA 1 cut(s) 141
BseBI CCWGG 3 cut(s) 23, 183, 881
BseDI CCNNGG 1 cut(s) 124
BseGI GGATG 5 cut(s) 103, 156, 555, 760, 1495
BseLI CCNNNNNNNGG 3 cut(s) 188, 961, 1165
BseMI GCAATG 4 cut(s) 298, 459, 909, 1210
BseMII CTCAG 3 cut(s) 72, 159, 1571
BseNI ACTGG 6 cut(s) 706, 723, 1014, 1139, 1300, 1387
BseSI GKGCMC 2 cut(s) 1279, 1378
BseXI GCAGC 5 cut(s) 275, 502, 627, 1094, 1244
BsgI GTGCAG 1 cut(s) 1101
Bsh1236I CGCG 1 cut(s) 1338
BshFI GGCC 5 cut(s) 386, 668, 719, 898, 1072
BshNI GGYRCC 1 cut(s) 1373
BsiHKAI GWGCWC 3 cut(s) 736, 807, 1279
BsiHKCI CYCGRG 1 cut(s) 976
BsiSI CCGG 1 cut(s) 142
BslI CCNNNNNNNGG 3 cut(s) 188, 961, 1165
BsmAI GTCTC 2 cut(s) 1307, 1504
BsmI GAATGC 2 cut(s) 618, 1134
BsnI GGCC 5 cut(s) 386, 668, 719, 898, 1072
BsoBI CYCGRG 1 cut(s) 976
Bsp119I TTCGAA 1 cut(s) 494
Bsp1286I GDGCHC 4 cut(s) 736, 807, 1279, 1378
Bsp13I TCCGGA 1 cut(s) 141
Bsp143I GATC 7 cut(s) 589, 601, 771, 869, 883, 969, 1285
BspACI CCGC 5 cut(s) 55, 58, 1216, 1336, 1429
BspANI GGCC 5 cut(s) 386, 668, 719, 898, 1072
BspCNI CTCAG 3 cut(s) 73, 160, 1570
BspEI TCCGGA 1 cut(s) 141
BspFNI CGCG 1 cut(s) 1338
BspHI TCATGA 1 cut(s) 379
BspLI GGNNCC 1 cut(s) 1375
BspMAI CTGCAG 4 cut(s) 265, 520, 1234, 1543
BspPI GGATC 1 cut(s) 891
BspT104I TTCGAA 1 cut(s) 494
BspT107I GGYRCC 1 cut(s) 1373
BsrBI CCGCTC 1 cut(s) 55
BsrDI GCAATG 4 cut(s) 298, 459, 909, 1210
BsrI ACTGG 6 cut(s) 706, 723, 1014, 1139, 1300, 1387
BssECI CCNNGG 1 cut(s) 124
BssMI GATC 7 cut(s) 589, 601, 771, 869, 883, 969, 1285
BssNAI GTATAC 1 cut(s) 1471
BssNI GRCGYC 2 cut(s) 110, 244
BssT1I CCWWGG 1 cut(s) 124
Bst1107I GTATAC 1 cut(s) 1471
Bst2UI CCWGG 3 cut(s) 23, 183, 881
Bst4CI ACNGT 6 cut(s) 279, 540, 646, 709, 809, 1120
Bst6I CTCTTC 3 cut(s) 35, 516, 1263
BstACI GRCGYC 2 cut(s) 110, 244
BstBI TTCGAA 1 cut(s) 494
BstC8I GCNNGC 1 cut(s) 597
BstDEI CTNAG 5 cut(s) 81, 168, 695, 873, 1557
BstF5I GGATG 5 cut(s) 103, 156, 555, 760, 1495
BstFNI CGCG 1 cut(s) 1338
BstKTI GATC 7 cut(s) 592, 604, 774, 872, 886, 972, 1288
BstMAI GTCTC 2 cut(s) 1307, 1504
BstMBI GATC 7 cut(s) 589, 601, 771, 869, 883, 969, 1285
BstMWI GCNNNNNNNGC 2 cut(s) 263, 665
BstNI CCWGG 3 cut(s) 23, 183, 881
BstNSI RCATGY 2 cut(s) 106, 328
BstSCI CCNGG 3 cut(s) 21, 181, 879
BstSFI CTRYAG 7 cut(s) 261, 331, 516, 567, 1074, 1230, 1539
BstSLI GKGCMC 2 cut(s) 1279, 1378
BstUI CGCG 1 cut(s) 1338
BstV1I GCAGC 5 cut(s) 275, 502, 627, 1094, 1244
BstV2I GAAGAC 2 cut(s) 1114, 1484
BstX2I RGATCY 2 cut(s) 771, 869
BstYI RGATCY 2 cut(s) 771, 869
BstZ17I GTATAC 1 cut(s) 1471
BsuI GTATCC 2 cut(s) 1166, 1402
BsuRI GGCC 5 cut(s) 386, 668, 719, 898, 1072
BtgZI GCGATG 1 cut(s) 1353
BtsCI GGATG 5 cut(s) 103, 156, 555, 760, 1495
BtsIMutI CAGTG 4 cut(s) 120, 339, 805, 1146
Cac8I GCNNGC 1 cut(s) 597
CciI TCATGA 1 cut(s) 379
Cfr13I GGNCC 1 cut(s) 877
Csp6I GTAC 3 cut(s) 205, 699, 981
CviAII CATG 5 cut(s) 103, 325, 380, 388, 1396
CviQI GTAC 3 cut(s) 205, 699, 981
DdeI CTNAG 5 cut(s) 81, 168, 695, 873, 1557
DpnI GATC 7 cut(s) 591, 603, 773, 871, 885, 971, 1287
DpnII GATC 7 cut(s) 589, 601, 771, 869, 883, 969, 1285
EaeI YGGCCR 1 cut(s) 717
Eam1104I CTCTTC 3 cut(s) 35, 516, 1263
EarI CTCTTC 3 cut(s) 35, 516, 1263
EciI GGCGGA 2 cut(s) 73, 1418
Eco130I CCWWGG 1 cut(s) 124
Eco32I GATATC 1 cut(s) 371
Eco47I GGWCC 1 cut(s) 877
Eco57I CTGAAG 3 cut(s) 363, 885, 1287
Eco88I CYCGRG 1 cut(s) 976
EcoRII CCWGG 3 cut(s) 21, 181, 879
EcoRV GATATC 1 cut(s) 371
EcoT14I CCWWGG 1 cut(s) 124
EcoT22I ATGCAT 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 124
FaeI CATG 5 cut(s) 106, 328, 383, 391, 1399
FalI AAGNNNNNCTT 2 cut(s) 758, 790
FatI CATG 5 cut(s) 102, 324, 379, 387, 1395
FblI GTMKAC 2 cut(s) 481, 1470
Fnu4HI GCNGC 8 cut(s) 56, 264, 516, 616, 1083, 1216, 1233, 1336
FokI GGATG 5 cut(s) 110, 163, 542, 767, 1482
Fsp4HI GCNGC 8 cut(s) 56, 264, 516, 616, 1083, 1216, 1233, 1336
FspBI CTAG 2 cut(s) 587, 1289
GluI GCNGC 8 cut(s) 56, 264, 516, 616, 1083, 1216, 1233, 1336
GsuI CTGGAG 3 cut(s) 204, 938, 1031
HaeIII GGCC 5 cut(s) 386, 668, 719, 898, 1072
HapII CCGG 1 cut(s) 142
Hin1I GRCGYC 2 cut(s) 110, 244
Hin1II CATG 5 cut(s) 106, 328, 383, 391, 1399
HindIII AAGCTT 3 cut(s) 134, 578, 1328
HinfI GANTC 4 cut(s) 230, 478, 491, 1044
HpaII CCGG 1 cut(s) 142
HphI GGTGA 2 cut(s) 964, 1003
Hpy166II GTNNAC 5 cut(s) 108, 118, 482, 1277, 1471
Hpy188I TCNGA 6 cut(s) 270, 417, 546, 822, 904, 1001
Hpy8I GTNNAC 5 cut(s) 108, 118, 482, 1277, 1471
Hpy99I CGWCG 1 cut(s) 249
HpyAV CCTTC 9 cut(s) 88, 338, 490, 496, 569, 760, 909, 1313, 1346
HpyCH4III ACNGT 6 cut(s) 279, 540, 646, 709, 809, 1120
HpyCH4IV ACGT 3 cut(s) 110, 244, 1516
HpyF10VI GCNNNNNNNGC 2 cut(s) 263, 665
HpyF3I CTNAG 5 cut(s) 81, 168, 695, 873, 1557
HpySE526I ACGT 3 cut(s) 110, 244, 1516
Hsp92I GRCGYC 2 cut(s) 110, 244
Hsp92II CATG 5 cut(s) 106, 328, 383, 391, 1399
Kpn2I TCCGGA 1 cut(s) 141
Kzo9I GATC 7 cut(s) 589, 601, 771, 869, 883, 969, 1285
LmnI GCTCC 2 cut(s) 262, 271
Lsp1109I GCAGC 5 cut(s) 275, 502, 627, 1094, 1244
LweI GCATC 3 cut(s) 303, 367, 421
MaeI CTAG 2 cut(s) 587, 1289
MaeII ACGT 3 cut(s) 110, 244, 1516
MaeIII GTNAC 3 cut(s) 725, 946, 1015
MalI GATC 7 cut(s) 591, 603, 773, 871, 885, 971, 1287
MbiI CCGCTC 1 cut(s) 55
MboI GATC 7 cut(s) 589, 601, 771, 869, 883, 969, 1285
MfeI CAATTG 2 cut(s) 234, 252
MflI RGATCY 2 cut(s) 771, 869
MhlI GDGCHC 4 cut(s) 736, 807, 1279, 1378
MlsI TGGCCA 1 cut(s) 719
MluNI TGGCCA 1 cut(s) 719
MlyI GAGTC 1 cut(s) 487
MmeI TCCRAC 3 cut(s) 207, 220, 277
MnlI CCTC 9 cut(s) 54, 67, 376, 985, 1006, 1167, 1264, 1364, 1566
Mox20I TGGCCA 1 cut(s) 719
Mph1103I ATGCAT 1 cut(s) 414
MroI TCCGGA 1 cut(s) 141
MscI TGGCCA 1 cut(s) 719
MseI TTAA 5 cut(s) 68, 209, 354, 399, 746
MslI CAYNNNNRTG 2 cut(s) 378, 1139
Msp20I TGGCCA 1 cut(s) 719
MspA1I CMGCKG 1 cut(s) 566
MspI CCGG 1 cut(s) 142
MspR9I CCNGG 3 cut(s) 23, 183, 881
MunI CAATTG 2 cut(s) 234, 252
Mva1269I GAATGC 2 cut(s) 618, 1134
MvaI CCWGG 3 cut(s) 23, 183, 881
MvnI CGCG 1 cut(s) 1338
MwoI GCNNNNNNNGC 2 cut(s) 263, 665
NdeII GATC 7 cut(s) 589, 601, 771, 869, 883, 969, 1285
NlaIII CATG 5 cut(s) 106, 328, 383, 391, 1399
NlaIV GGNNCC 1 cut(s) 1375
NmeAIII GCCGAG 1 cut(s) 1457
NmuCI GTSAC 1 cut(s) 946
NsiI ATGCAT 1 cut(s) 414
NspI RCATGY 2 cut(s) 106, 328
NspV TTCGAA 1 cut(s) 494
PaeR7I CTCGAG 1 cut(s) 976
PagI TCATGA 1 cut(s) 379
PciI ACATGT 2 cut(s) 102, 324
PctI GAATGC 2 cut(s) 618, 1134
PfeI GAWTC 3 cut(s) 230, 491, 1044
PflFI GACNNNGTC 2 cut(s) 246, 1515
PkrI GCNGC 8 cut(s) 57, 265, 517, 617, 1084, 1217, 1234, 1337
PleI GAGTC 1 cut(s) 486
PpsI GAGTC 1 cut(s) 486
PscI ACATGT 2 cut(s) 102, 324
PshBI ATTAAT 1 cut(s) 399
Psp6I CCWGG 3 cut(s) 21, 181, 879
PspGI CCWGG 3 cut(s) 21, 181, 879
PspN4I GGNNCC 1 cut(s) 1375
PspPI GGNCC 1 cut(s) 877
PspXI VCTCGAGB 1 cut(s) 976
PstI CTGCAG 4 cut(s) 265, 520, 1234, 1543
PsuI RGATCY 2 cut(s) 771, 869
PsyI GACNNNGTC 2 cut(s) 246, 1515
PvuII CAGCTG 1 cut(s) 566
RsaI GTAC 3 cut(s) 206, 700, 982
RsaNI GTAC 3 cut(s) 205, 699, 981
RseI CAYNNNNRTG 2 cut(s) 378, 1139
SaqAI TTAA 5 cut(s) 68, 209, 354, 399, 746
SatI GCNGC 8 cut(s) 56, 264, 516, 616, 1083, 1216, 1233, 1336
Sau3AI GATC 7 cut(s) 589, 601, 771, 869, 883, 969, 1285
Sau96I GGNCC 1 cut(s) 877
ScaI AGTACT 2 cut(s) 700, 982
SchI GAGTC 1 cut(s) 487
ScrFI CCNGG 3 cut(s) 23, 183, 881
SduI GDGCHC 4 cut(s) 736, 807, 1279, 1378
SfaNI GCATC 3 cut(s) 303, 367, 421
SfcI CTRYAG 7 cut(s) 261, 331, 516, 567, 1074, 1230, 1539
Sfr274I CTCGAG 1 cut(s) 976
SfuI TTCGAA 1 cut(s) 494
SinI GGWCC 1 cut(s) 877
SlaI CTCGAG 1 cut(s) 976
SmiMI CAYNNNNRTG 2 cut(s) 378, 1139
SmlI CTYRAG 1 cut(s) 976
SmoI CTYRAG 1 cut(s) 976
SsiI CCGC 5 cut(s) 55, 58, 1216, 1336, 1429
SspI AATATT 1 cut(s) 1534
SspMI CTAG 2 cut(s) 587, 1289
StyD4I CCNGG 3 cut(s) 21, 181, 879
StyI CCWWGG 1 cut(s) 124
TaaI ACNGT 6 cut(s) 279, 540, 646, 709, 809, 1120
TaiI ACGT 3 cut(s) 113, 247, 1519
TaqI TCGA 5 cut(s) 361, 489, 494, 977, 1042
TatI WGTACW 2 cut(s) 698, 980
TauI GCSGC 3 cut(s) 58, 1218, 1338
TfiI GAWTC 3 cut(s) 230, 491, 1044
Tru1I TTAA 5 cut(s) 68, 209, 354, 399, 746
Tru9I TTAA 5 cut(s) 68, 209, 354, 399, 746
TscAI CASTG 4 cut(s) 120, 346, 812, 1146
TseFI GTSAC 1 cut(s) 946
TseI GCWGC 5 cut(s) 263, 515, 615, 1082, 1232
Tsp45I GTSAC 1 cut(s) 946
TspDTI ATGAA 6 cut(s) 368, 417, 563, 1114, 1475, 1484
TspGWI ACGGA 2 cut(s) 1148, 1186
TspRI CASTG 4 cut(s) 120, 346, 812, 1146
Tth111I GACNNNGTC 2 cut(s) 246, 1515
VneI GTGCAC 1 cut(s) 1275
VpaK11BI GGWCC 1 cut(s) 877
VspI ATTAAT 1 cut(s) 399
XapI RAATTY 2 cut(s) 923, 1523
XbaI TCTAGA 1 cut(s) 1288
XceI RCATGY 2 cut(s) 106, 328
XhoI CTCGAG 1 cut(s) 976
XmiI GTMKAC 2 cut(s) 481, 1470
XspI CTAG 2 cut(s) 587, 1289
ZraI GACGTC 2 cut(s) 111, 245
ZrmI AGTACT 2 cut(s) 700, 982
Zsp2I ATGCAT 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.