Rroxscaffold_7G00169890

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007203
Physical Location & Seq
Reverse (-)
10376761 .. 10383653
6893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00169890.1

Sequence Viewer

Length: 1818 bp
ATGAACCGACTTCCCCAAAATCAAGTCCCCGACGCCGGCACAGGCCTTGAGAACAAAAGGGTAGGTGAATTTATTAGGCAAGACTTCACCTTTAATCATAACTCTGTAAAAGCACAAAGCTTTATGCTTTGCATGACCGGTCTGAGCGTCGGAGGCGAACTCCGGCAACGTCGCGTCTTGTTGAGCAGGGGAAGCTGCAACACGATCCGAACCAAGAAGCGGTGGCTTCGCAATTGGAGAACTTGCTGGGGGAGGTTAGAGCAGTATGAGAGAGATATGGAGGAATATCATACAAGCCTGGCGAATTGGGAGAAGAAGAGAGAGAATGAGCGGCGGAGGATTTTAATGGAGGAAGCTGAGAGTAAGCAGAAGGATGACATGTGGACGTCAGTGAACAACCAAGGGAATAAGCTTTTCCAGAGATGGATGTCCAAGAAAAGACCTGCGAATGTAGAACCTGGAGTTGGGAAATGGGTTTCGTACTTAAATAGAGAGAGGCAGTTGGATTCAATTGTTGGACGTCGTCCAATTGCTCCTGCAGCTCCGAAAGGACTGTATATTTATGGCAATGTTGGAAGCGGAAAGACGATGCTTATGGACATGTTCTATAGTGCCACTGAAGGAATAGTTAAGCATCGAAAGAGATATCACTTTCATGAGGCTATGTTGAAAATTAATGAACAAATGCATCAGATTTGGAAAAATCAAGTGGCAGATAAGTCTTTGCAGTCCAGCATTGCTAATTGGATAATGAGTCTACCCTTCGATTCGAAGGTCAAGGAGTGGGTGGCTGCAGAAGAGAACTACAAACAACAGGTTCAGATGAAAAACATCCTTCCAGCTGTAGCAGATAAGCTTCTCCTAGATCGGCAGGCAGATCAAAAGGGTGGCAGCATTCTTTGTTTTGATGAGATACAGACTGTTGATGTATTTGCTATTGTGGCCTTATCTGGAATTGTTAGCAGATTGCTAAGTACTGGAACTGTTTTTGTGGCCACCAGTAACCGAGCACCAACGGACTTAAATCAGGATGGTATGCAGAAGGAGATCTTCCAAAAGTTTGTTGCCAAATTGAATGAGCACTGTGAGAATGTTCTGATTGGAAGTGAGATAGATTATCGCCGTGTTATAGCTCAAAATCAAAGATCTAAGGACCAGGATCACTACTTCTGGCCTTCAGACAGCATTGCTCTCGAGAAATTTGAGAGAAAGTGGCAAGAAGTCACAAGCCAAGTAGGGGAACAGATCACCTCGAGTACTATTTCGGTGATGTTTGGAAGAAAGCTGGAGGTTACTGAAAGCTGTAATGGAGTAGCACGATTCACATTTGAATATCTATGTGGTCGGCCTGTAGGTGCAGCAGATTATATTGCAGTAGCAGAAAATTATCATACGGTCTTCATATCTGGCATTCCAGTGATGAGTATGCGTATCCGTGATAAGGCACGGAGGTTTATTACCCTTGTTGATGAGTTATACAATCATCATTGCCGCCTATTTTGTTCTGCAGCTTCTTCTATTGATGATTTGTTTCAAGGAACTGAAGAGGGTGCACTTTTTGATCTAGAGAGTTTCCAGTTTGAAACAGAGACAGAAGGTGCAAAGCTTCGCCGCGATGTTTTAGCAGAAGGGAATGTGAGTTCAGGAGGTGCCCCAACTGGTATAGTTTCCATGCTATCTGGTCAAGAAGAGATGTTTGCTTTCCGCCGAGCAGTTTCACGACTGATTGAAATGCAGACACCTGTATACTTGGGGGAAGTATCCTCTTCGATCAGGATTGCACCAACTCTACCACAATGTAGAGCCCGAGGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

605

Amino Acids

69.18

Weight (kDa)

8.9

Isoelectric Point (pI)

47.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 167 - 515 3.3e-64 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 389, 523
Acc36I ACCTGC 1 cut(s) 451
AccB1I GGYRCC 1 cut(s) 1649
AccBSI CCGCTC 1 cut(s) 331
AccI GTMKAC 2 cut(s) 757, 1746
AccII CGCG 2 cut(s) 174, 1614
AciI CCGC 7 cut(s) 220, 331, 334, 579, 1492, 1612, 1705
AclWI GGATC 2 cut(s) 199, 1167
AcoI YGGCCR 1 cut(s) 993
AcsI RAATTY 2 cut(s) 68, 1199
AcuI CTGAAG 3 cut(s) 639, 1161, 1563
AcyI GRCGYC 3 cut(s) 33, 386, 520
AfaI GTAC 3 cut(s) 482, 976, 1258
AfiI CCNNNNNNNGG 5 cut(s) 35, 219, 464, 1237, 1441
AflIII ACRYGT 2 cut(s) 378, 600
AgeI ACCGGT 1 cut(s) 137
AgsI TTSAA 7 cut(s) 510, 670, 1075, 1331, 1535, 1583, 1730
AjnI CCWGG 3 cut(s) 297, 457, 1155
AjuI GAANNNNNNNTTGG 2 cut(s) 447, 479
AloI GAACNNNNNNTCC 2 cut(s) 1624, 1656
Alw21I GWGCWC 3 cut(s) 1012, 1083, 1555
Alw26I GTCTC 1 cut(s) 1583
Alw44I GTGCAC 1 cut(s) 1551
AlwI GGATC 2 cut(s) 199, 1167
Ama87I CYCGRG 3 cut(s) 1193, 1252, 1806
AoxI GGCC 5 cut(s) 43, 942, 993, 1172, 1346
ApaLI GTGCAC 1 cut(s) 1551
ApeKI GCWGC 6 cut(s) 195, 539, 791, 891, 1358, 1508
ApoI RAATTY 2 cut(s) 68, 1199
AseI ATTAAT 1 cut(s) 675
AsiGI ACCGGT 1 cut(s) 137
AspS9I GGNCC 1 cut(s) 1153
AsuHPI GGTGA 4 cut(s) 77, 79, 1240, 1279
AsuII TTCGAA 1 cut(s) 770
AvaI CYCGRG 3 cut(s) 1193, 1252, 1806
AvaII GGWCC 1 cut(s) 1153
BaeGI GKGCMC 2 cut(s) 1555, 1654
BalI TGGCCA 1 cut(s) 995
BanI GGYRCC 1 cut(s) 1649
BanII GRGCYC 1 cut(s) 1807
BbsI GAAGAC 1 cut(s) 1390
Bbv12I GWGCWC 3 cut(s) 1012, 1083, 1555
BbvI GCAGC 6 cut(s) 182, 551, 778, 903, 1370, 1520
BccI CCATC 2 cut(s) 417, 1025
BceAI ACGGC 1 cut(s) 1107
BcgI CGANNNNNNTGC 2 cut(s) 1174, 1208
BciT130I CCWGG 3 cut(s) 299, 459, 1157
BciVI GTATCC 2 cut(s) 1442, 1771
BcoDI GTCTC 1 cut(s) 1583
BfaI CTAG 2 cut(s) 863, 1565
BfmI CTRYAG 6 cut(s) 537, 607, 792, 843, 1350, 1506
BfuAI ACCTGC 1 cut(s) 451
BfuI GTATCC 2 cut(s) 1442, 1771
BglII AGATCT 2 cut(s) 1047, 1145
BisI GCNGC 9 cut(s) 196, 332, 540, 792, 892, 1359, 1492, 1509, 1612
BlsI GCNGC 9 cut(s) 197, 333, 541, 793, 893, 1360, 1493, 1510, 1613
BmcAI AGTACT 2 cut(s) 976, 1258
Bme1390I CCNGG 3 cut(s) 299, 459, 1157
Bme18I GGWCC 1 cut(s) 1153
BmeT110I CYCGRG 3 cut(s) 1193, 1252, 1806
BmgT120I GGNCC 1 cut(s) 1153
BmiI GGNNCC 1 cut(s) 1651
BmrFI CCNGG 3 cut(s) 299, 459, 1157
BmsI GCATC 3 cut(s) 579, 643, 697
BpiI GAAGAC 1 cut(s) 1390
BplI GAGNNNNNCTC 2 cut(s) 144, 176
BpmI CTGGAG 2 cut(s) 480, 1307
Bpu14I TTCGAA 1 cut(s) 770
BpuEI CTTGAG 1 cut(s) 68
BsaHI GRCGYC 3 cut(s) 33, 386, 520
BsaJI CCNNGG 2 cut(s) 400, 1807
BsaWI WCCGGW 1 cut(s) 137
Bsc4I CCNNNNNNNGG 5 cut(s) 35, 219, 464, 1237, 1441
Bse118I RCCGGY 2 cut(s) 35, 137
Bse1I ACTGG 5 cut(s) 982, 999, 1415, 1576, 1663
Bse3DI GCAATG 4 cut(s) 574, 735, 1185, 1486
BseBI CCWGG 3 cut(s) 299, 459, 1157
BseDI CCNNGG 2 cut(s) 400, 1807
BseGI GGATG 4 cut(s) 379, 432, 831, 1036
BseLI CCNNNNNNNGG 5 cut(s) 35, 219, 464, 1237, 1441
BseMI GCAATG 4 cut(s) 574, 735, 1185, 1486
BseMII CTCAG 2 cut(s) 134, 348
BseNI ACTGG 5 cut(s) 982, 999, 1415, 1576, 1663
BseSI GKGCMC 2 cut(s) 1555, 1654
BseXI GCAGC 6 cut(s) 182, 551, 778, 903, 1370, 1520
BseYI CCCAGC 1 cut(s) 246
BsgI GTGCAG 1 cut(s) 1377
Bsh1236I CGCG 2 cut(s) 174, 1614
BshFI GGCC 5 cut(s) 45, 944, 995, 1174, 1348
BshNI GGYRCC 1 cut(s) 1649
BshTI ACCGGT 1 cut(s) 137
BsiHKAI GWGCWC 3 cut(s) 1012, 1083, 1555
BsiHKCI CYCGRG 3 cut(s) 1193, 1252, 1806
BsiSI CCGG 3 cut(s) 36, 138, 163
BslFI GGGAC 1 cut(s) 11
BslI CCNNNNNNNGG 5 cut(s) 35, 219, 464, 1237, 1441
BsmAI GTCTC 1 cut(s) 1583
BsmFI GGGAC 1 cut(s) 11
BsmI GAATGC 2 cut(s) 894, 1410
BsnI GGCC 5 cut(s) 45, 944, 995, 1174, 1348
BsoBI CYCGRG 3 cut(s) 1193, 1252, 1806
Bsp119I TTCGAA 1 cut(s) 770
Bsp1286I GDGCHC 5 cut(s) 1012, 1083, 1555, 1654, 1807
Bsp143I GATC 9 cut(s) 204, 865, 877, 1047, 1145, 1159, 1245, 1561, 1770
BspACI CCGC 7 cut(s) 220, 331, 334, 579, 1492, 1612, 1705
BspANI GGCC 5 cut(s) 45, 944, 995, 1174, 1348
BspCNI CTCAG 2 cut(s) 135, 349
BspFNI CGCG 2 cut(s) 174, 1614
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 1 cut(s) 1651
BspMAI CTGCAG 3 cut(s) 541, 796, 1510
BspMI ACCTGC 1 cut(s) 451
BspPI GGATC 2 cut(s) 199, 1167
BspT104I TTCGAA 1 cut(s) 770
BspT107I GGYRCC 1 cut(s) 1649
BsrBI CCGCTC 1 cut(s) 331
BsrDI GCAATG 4 cut(s) 574, 735, 1185, 1486
BsrFI RCCGGY 2 cut(s) 35, 137
BsrI ACTGG 5 cut(s) 982, 999, 1415, 1576, 1663
BssAI RCCGGY 2 cut(s) 35, 137
BssECI CCNNGG 2 cut(s) 400, 1807
BssMI GATC 9 cut(s) 204, 865, 877, 1047, 1145, 1159, 1245, 1561, 1770
BssNAI GTATAC 1 cut(s) 1747
BssNI GRCGYC 3 cut(s) 33, 386, 520
BssT1I CCWWGG 1 cut(s) 400
Bst1107I GTATAC 1 cut(s) 1747
Bst2UI CCWGG 3 cut(s) 299, 459, 1157
Bst4CI ACNGT 5 cut(s) 555, 922, 985, 1085, 1396
Bst6I CTCTTC 5 cut(s) 311, 792, 1539, 1683, 1771
BstACI GRCGYC 3 cut(s) 33, 386, 520
BstBI TTCGAA 1 cut(s) 770
BstC8I GCNNGC 2 cut(s) 37, 873
BstDEI CTNAG 4 cut(s) 143, 357, 971, 1149
BstF5I GGATG 4 cut(s) 379, 432, 831, 1036
BstFNI CGCG 2 cut(s) 174, 1614
BstKTI GATC 9 cut(s) 207, 868, 880, 1050, 1148, 1162, 1248, 1564, 1773
BstMAI GTCTC 1 cut(s) 1583
BstMBI GATC 9 cut(s) 204, 865, 877, 1047, 1145, 1159, 1245, 1561, 1770
BstMWI GCNNNNNNNGC 4 cut(s) 153, 192, 539, 941
BstNI CCWGG 3 cut(s) 299, 459, 1157
BstNSI RCATGY 2 cut(s) 382, 604
BstSCI CCNGG 3 cut(s) 297, 457, 1155
BstSFI CTRYAG 6 cut(s) 537, 607, 792, 843, 1350, 1506
BstSLI GKGCMC 2 cut(s) 1555, 1654
BstUI CGCG 2 cut(s) 174, 1614
BstV1I GCAGC 6 cut(s) 182, 551, 778, 903, 1370, 1520
BstV2I GAAGAC 1 cut(s) 1390
BstX2I RGATCY 2 cut(s) 1047, 1145
BstYI RGATCY 2 cut(s) 1047, 1145
BstZ17I GTATAC 1 cut(s) 1747
BsuI GTATCC 2 cut(s) 1442, 1771
BsuRI GGCC 5 cut(s) 45, 944, 995, 1174, 1348
BtgZI GCGATG 1 cut(s) 1629
BtsCI GGATG 4 cut(s) 379, 432, 831, 1036
BtsIMutI CAGTG 4 cut(s) 396, 615, 1081, 1422
BveI ACCTGC 1 cut(s) 451
Cac8I GCNNGC 2 cut(s) 37, 873
CciI TCATGA 1 cut(s) 655
Cfr10I RCCGGY 2 cut(s) 35, 137
Cfr13I GGNCC 1 cut(s) 1153
CseI GACGC 3 cut(s) 41, 136, 163
Csp6I GTAC 3 cut(s) 481, 975, 1257
CspAI ACCGGT 1 cut(s) 137
CviAII CATG 5 cut(s) 133, 379, 601, 656, 1672
CviQI GTAC 3 cut(s) 481, 975, 1257
DdeI CTNAG 4 cut(s) 143, 357, 971, 1149
DpnI GATC 9 cut(s) 206, 867, 879, 1049, 1147, 1161, 1247, 1563, 1772
DpnII GATC 9 cut(s) 204, 865, 877, 1047, 1145, 1159, 1245, 1561, 1770
EaeI YGGCCR 1 cut(s) 993
Eam1104I CTCTTC 5 cut(s) 311, 792, 1539, 1683, 1771
EarI CTCTTC 5 cut(s) 311, 792, 1539, 1683, 1771
EciI GGCGGA 2 cut(s) 349, 1694
Eco130I CCWWGG 1 cut(s) 400
Eco147I AGGCCT 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 1807
Eco32I GATATC 1 cut(s) 647
Eco47I GGWCC 1 cut(s) 1153
Eco57I CTGAAG 3 cut(s) 639, 1161, 1563
Eco88I CYCGRG 3 cut(s) 1193, 1252, 1806
EcoRII CCWGG 3 cut(s) 297, 457, 1155
EcoRV GATATC 1 cut(s) 647
EcoT14I CCWWGG 1 cut(s) 400
EcoT22I ATGCAT 1 cut(s) 690
EcoT38I GRGCYC 1 cut(s) 1807
ErhI CCWWGG 1 cut(s) 400
FaeI CATG 5 cut(s) 136, 382, 604, 659, 1675
FalI AAGNNNNNCTT 2 cut(s) 1034, 1066
FaqI GGGAC 1 cut(s) 11
FatI CATG 5 cut(s) 132, 378, 600, 655, 1671
FblI GTMKAC 2 cut(s) 757, 1746
Fnu4HI GCNGC 9 cut(s) 196, 332, 540, 792, 892, 1359, 1492, 1509, 1612
FokI GGATG 4 cut(s) 386, 439, 818, 1043
FriOI GRGCYC 1 cut(s) 1807
Fsp4HI GCNGC 9 cut(s) 196, 332, 540, 792, 892, 1359, 1492, 1509, 1612
FspBI CTAG 2 cut(s) 863, 1565
GluI GCNGC 9 cut(s) 196, 332, 540, 792, 892, 1359, 1492, 1509, 1612
GsaI CCCAGC 1 cut(s) 250
GsuI CTGGAG 2 cut(s) 480, 1307
HaeIII GGCC 5 cut(s) 45, 944, 995, 1174, 1348
HapII CCGG 3 cut(s) 36, 138, 163
HgaI GACGC 3 cut(s) 41, 136, 163
Hin1I GRCGYC 3 cut(s) 33, 386, 520
Hin1II CATG 5 cut(s) 136, 382, 604, 659, 1675
HindIII AAGCTT 4 cut(s) 118, 410, 854, 1604
HinfI GANTC 4 cut(s) 506, 754, 767, 1320
HpaII CCGG 3 cut(s) 36, 138, 163
HphI GGTGA 4 cut(s) 77, 79, 1240, 1279
Hpy166II GTNNAC 5 cut(s) 384, 394, 758, 1553, 1747
Hpy188I TCNGA 8 cut(s) 144, 152, 209, 546, 693, 822, 1098, 1180
Hpy8I GTNNAC 5 cut(s) 384, 394, 758, 1553, 1747
Hpy99I CGWCG 4 cut(s) 35, 152, 174, 525
HpyAV CCTTC 9 cut(s) 364, 614, 766, 772, 845, 1036, 1185, 1589, 1622
HpyCH4III ACNGT 5 cut(s) 555, 922, 985, 1085, 1396
HpyCH4IV ACGT 3 cut(s) 169, 386, 520
HpyF10VI GCNNNNNNNGC 4 cut(s) 153, 192, 539, 941
HpyF3I CTNAG 4 cut(s) 143, 357, 971, 1149
HpySE526I ACGT 3 cut(s) 169, 386, 520
Hsp92I GRCGYC 3 cut(s) 33, 386, 520
Hsp92II CATG 5 cut(s) 136, 382, 604, 659, 1675
KroI GCCGGC 1 cut(s) 35
KroNI GCCGGC 1 cut(s) 37
Kzo9I GATC 9 cut(s) 204, 865, 877, 1047, 1145, 1159, 1245, 1561, 1770
LmnI GCTCC 2 cut(s) 538, 547
Lsp1109I GCAGC 6 cut(s) 182, 551, 778, 903, 1370, 1520
LweI GCATC 3 cut(s) 579, 643, 697
MaeI CTAG 2 cut(s) 863, 1565
MaeII ACGT 3 cut(s) 169, 386, 520
MaeIII GTNAC 3 cut(s) 1001, 1222, 1291
MalI GATC 9 cut(s) 206, 867, 879, 1049, 1147, 1161, 1247, 1563, 1772
MbiI CCGCTC 1 cut(s) 331
MboI GATC 9 cut(s) 204, 865, 877, 1047, 1145, 1159, 1245, 1561, 1770
MfeI CAATTG 3 cut(s) 232, 510, 528
MflI RGATCY 2 cut(s) 1047, 1145
MhlI GDGCHC 5 cut(s) 1012, 1083, 1555, 1654, 1807
MlsI TGGCCA 1 cut(s) 995
MluNI TGGCCA 1 cut(s) 995
MlyI GAGTC 1 cut(s) 763
MmeI TCCRAC 4 cut(s) 130, 483, 496, 553
Mox20I TGGCCA 1 cut(s) 995
Mph1103I ATGCAT 1 cut(s) 690
MroNI GCCGGC 1 cut(s) 35
MscI TGGCCA 1 cut(s) 995
MseI TTAA 6 cut(s) 93, 344, 485, 630, 675, 1022
MslI CAYNNNNRTG 2 cut(s) 654, 1415
Msp20I TGGCCA 1 cut(s) 995
MspA1I CMGCKG 1 cut(s) 842
MspI CCGG 3 cut(s) 36, 138, 163
MspR9I CCNGG 3 cut(s) 299, 459, 1157
MunI CAATTG 3 cut(s) 232, 510, 528
Mva1269I GAATGC 2 cut(s) 894, 1410
MvaI CCWGG 3 cut(s) 299, 459, 1157
MvnI CGCG 2 cut(s) 174, 1614
MwoI GCNNNNNNNGC 4 cut(s) 153, 192, 539, 941
NaeI GCCGGC 1 cut(s) 37
NdeII GATC 9 cut(s) 204, 865, 877, 1047, 1145, 1159, 1245, 1561, 1770
NgoMIV GCCGGC 1 cut(s) 35
NlaIII CATG 5 cut(s) 136, 382, 604, 659, 1675
NlaIV GGNNCC 1 cut(s) 1651
NmeAIII GCCGAG 1 cut(s) 1733
NmuCI GTSAC 1 cut(s) 1222
NsiI ATGCAT 1 cut(s) 690
NspI RCATGY 2 cut(s) 382, 604
NspV TTCGAA 1 cut(s) 770
PaeR7I CTCGAG 2 cut(s) 1193, 1252
PagI TCATGA 1 cut(s) 655
PceI AGGCCT 1 cut(s) 45
PciI ACATGT 2 cut(s) 378, 600
PctI GAATGC 2 cut(s) 894, 1410
PdiI GCCGGC 1 cut(s) 37
PfeI GAWTC 3 cut(s) 506, 767, 1320
PflFI GACNNNGTC 1 cut(s) 522
PinAI ACCGGT 1 cut(s) 137
PkrI GCNGC 9 cut(s) 197, 333, 541, 793, 893, 1360, 1493, 1510, 1613
PleI GAGTC 1 cut(s) 762
PpsI GAGTC 1 cut(s) 762
PscI ACATGT 2 cut(s) 378, 600
PshBI ATTAAT 1 cut(s) 675
Psp6I CCWGG 3 cut(s) 297, 457, 1155
PspFI CCCAGC 1 cut(s) 246
PspGI CCWGG 3 cut(s) 297, 457, 1155
PspN4I GGNNCC 1 cut(s) 1651
PspPI GGNCC 1 cut(s) 1153
PspXI VCTCGAGB 1 cut(s) 1252
PstI CTGCAG 3 cut(s) 541, 796, 1510
PsuI RGATCY 2 cut(s) 1047, 1145
PsyI GACNNNGTC 1 cut(s) 522
PvuII CAGCTG 1 cut(s) 842
RsaI GTAC 3 cut(s) 482, 976, 1258
RsaNI GTAC 3 cut(s) 481, 975, 1257
RseI CAYNNNNRTG 2 cut(s) 654, 1415
SaqAI TTAA 6 cut(s) 93, 344, 485, 630, 675, 1022
SatI GCNGC 9 cut(s) 196, 332, 540, 792, 892, 1359, 1492, 1509, 1612
Sau3AI GATC 9 cut(s) 204, 865, 877, 1047, 1145, 1159, 1245, 1561, 1770
Sau96I GGNCC 1 cut(s) 1153
ScaI AGTACT 2 cut(s) 976, 1258
SchI GAGTC 1 cut(s) 763
ScrFI CCNGG 3 cut(s) 299, 459, 1157
SduI GDGCHC 5 cut(s) 1012, 1083, 1555, 1654, 1807
SfaNI GCATC 3 cut(s) 579, 643, 697
SfcI CTRYAG 6 cut(s) 537, 607, 792, 843, 1350, 1506
Sfr274I CTCGAG 2 cut(s) 1193, 1252
SfuI TTCGAA 1 cut(s) 770
SinI GGWCC 1 cut(s) 1153
SlaI CTCGAG 2 cut(s) 1193, 1252
SmiMI CAYNNNNRTG 2 cut(s) 654, 1415
SmlI CTYRAG 3 cut(s) 47, 1193, 1252
SmoI CTYRAG 3 cut(s) 47, 1193, 1252
SseBI AGGCCT 1 cut(s) 45
SsiI CCGC 7 cut(s) 220, 331, 334, 579, 1492, 1612, 1705
SspMI CTAG 2 cut(s) 863, 1565
StuI AGGCCT 1 cut(s) 45
StyD4I CCNGG 3 cut(s) 297, 457, 1155
StyI CCWWGG 1 cut(s) 400
TaaI ACNGT 5 cut(s) 555, 922, 985, 1085, 1396
TaiI ACGT 3 cut(s) 172, 389, 523
TaqI TCGA 6 cut(s) 637, 765, 770, 1194, 1253, 1769
TatI WGTACW 2 cut(s) 974, 1256
TauI GCSGC 3 cut(s) 334, 1494, 1614
TfiI GAWTC 3 cut(s) 506, 767, 1320
Tru1I TTAA 6 cut(s) 93, 344, 485, 630, 675, 1022
Tru9I TTAA 6 cut(s) 93, 344, 485, 630, 675, 1022
TscAI CASTG 4 cut(s) 396, 622, 1088, 1422
TseFI GTSAC 1 cut(s) 1222
TseI GCWGC 6 cut(s) 195, 539, 791, 891, 1358, 1508
Tsp45I GTSAC 1 cut(s) 1222
TspDTI ATGAA 5 cut(s) 17, 644, 693, 839, 1390
TspGWI ACGGA 3 cut(s) 1031, 1424, 1462
TspRI CASTG 4 cut(s) 396, 622, 1088, 1422
Tth111I GACNNNGTC 1 cut(s) 522
VneI GTGCAC 1 cut(s) 1551
VpaK11BI GGWCC 1 cut(s) 1153
VspI ATTAAT 1 cut(s) 675
XapI RAATTY 2 cut(s) 68, 1199
XbaI TCTAGA 1 cut(s) 1564
XceI RCATGY 2 cut(s) 382, 604
XhoI CTCGAG 2 cut(s) 1193, 1252
XmiI GTMKAC 2 cut(s) 757, 1746
XspI CTAG 2 cut(s) 863, 1565
ZraI GACGTC 2 cut(s) 387, 521
ZrmI AGTACT 2 cut(s) 976, 1258
Zsp2I ATGCAT 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.