AT2G27040

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006994
Physical Location & Seq
Reverse (-)
11536502 .. 11542391
5890 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27040.2

Sequence Viewer

Length: 2775 bp
ATGGATTCAACAAATGGTAACGGAGCTGATCTTGAATCAGCAAATGGGGCAAACGGGAGTGGGGTTACTGAGGCATTACCACCTCCTCCACCAGTTATACCTCCAAATGTGGAACCAGTTCGTGTTAAAACTGAACTTGCTGAGAAGAAGGGGCCAGTTCGAGTTCCTATGGCTCGAAAAGGATTTGGAACAAGGGGCCAAAAGATCCCCTTGTTAACAAATCATTTCAAAGTCGATGTGGCTAATCTTCAGGGTCATTTCTTCCACTACAGTGTGGCTCTATTCTATGATGATGGTCGTCCTGTTGAACAAAAGGGTGTTGGAAGAAAAATCCTTGACAAGGTGCATCAGACTTACCATTCTGATCTGGATGGTAAAGAGTTTGCTTATGACGGTGAGAAGACGTTGTTTACATATGGAGCTTTGCCTAGTAACAAGATGGATTTTTCTGTGGTGCTTGAGGAAGTATCTGCTACAAGGGCTAATGGAAACGGAAGCCCCAATGGGAATGAAAGTCCAAGTGATGGTGATAGGAAAAGACTGCGTAGGCCTAACCGGTCCAAAAACTTTAGAGTGGAGATCAGCTATGCGGCCAAAATTCCTCTTCAAGCTCTTGCTAATGCAATGCGGGGACAAGAATCAGAGAATTCCCAGGAGGCAATACGGGTTCTTGATATCATATTGAGGCAACATGCTGCTAGACAAGGTTGCTTGCTTGTTCGACAGTCTTTTTTCCACAATGATCCAACCAACTGTGAACCAGTTGGTGGTAACATCTTAGGATGTAGGGGATTTCACTCCAGTTTCAGAACAACGCAGGGTGGCATGTCACTTAATATGGATGTTACAACCACCATGATCATCAAGCCTGGTCCAGTGGTTGATTTCCTAATTGCTAACCAAAATGCTAGGGACCCTTATTCGATTGACTGGTCTAAGGCTAAACGAACCCTTAAGAACCTAAGGGTAAAGGTCAGCCCCTCAGGCCAAGAATTCAAGATAACCGGATTGAGTGACAAGCCTTGCAGGGAACAAACGTTTGAATTGAAGAAAAGGAACCCAAATGAAAATGGAGAGTTCGAAACTACTGAAGTTACAGTTGCTGACTACTTCCGCGATACAAGGCATATTGATTTGCAATATTCTGCGGATTTGCCTTGCATCAATGTTGGGAAGCCAAAGCGACCCACTTACATTCCTCTCGAGCTCTGCGCGTTGGTTCCACTTCAGAGGTACACAAAAGCACTTACCACGTTCCAAAGATCTGCCCTTGTTGAGAAATCCAGACAGAAACCCCAAGAGAGGATGACTGTTCTGTCCAAAGCTCTGAAAGTTAGCAACTATGATGCGGAACCACTCCTGCGATCCTGTGGCATTTCGATCAGCTCCAACTTTACTCAGGTGGAGGGTCGTGTTCTACCAGCTCCCAAGCTGAAAATGGGATGTGGATCTGAAACCTTTCCCAGAAATGGTCGCTGGAACTTCAACAACAAGGAATTTGTTGAGCCCACCAAAATTCAACGATGGGTTGTTGTCAATTTCTCTGCTCGCTGTAATGTACGTCAAGTTGTTGATGATCTGATAAAAATTGGAGGATCAAAAGGAATTGAAATTGCTTCTCCCTTTCAAGTGTTTGAGGAGGGTAATCAATTCCGCCGTGCTCCTCCTATGATTCGTGTTGAGAACATGTTTAAGGACATCCAATCGAAACTCCCTGGTGTCCCACAATTCATACTATGTGTGCTCCCTGACAAAAAGAACAGTGATCTCTATGGTCCATGGAAGAAAAAAAACTTAACTGAATTTGGCATTGTTACTCAATGCATGGCTCCAACGCGGCAACCTAATGATCAGTATCTTACTAACTTACTTCTGAAGATTAATGCAAAGCTTGGAGGCCTGAACTCAATGTTAAGTGTAGAGCGTACACCTGCGTTCACTGTGATTTCTAAGGTTCCAACCATTATCCTTGGGATGGATGTTTCACATGGATCTCCTGGACAGTCTGATGTCCCGTCCATCGCTGCTGTGGTGAGTTCTAGGGAGTGGCCACTGATATCCAAATATAGAGCATCTGTTCGGACACAGCCTTCTAAGGCTGAGATGATTGAGTCCCTTGTCAAGAAAAATGGAACTGAAGACGATGGCATTATCAAGGAGTTGCTGGTAGATTTCTACACCAGCTCGAATAAGAGAAAACCAGAGCATATCATAATTTTCAGGGATGGTGTGAGTGAATCTCAATTCAATCAGGTTCTGAATATTGAACTTGATCAGATCATCGAGGCTTGCAAGCTCTTAGACGCAAATTGGAACCCAAAGTTCCTTTTGTTGGTGGCTCAAAAGAATCATCATACCAAGTTCTTCCAGCCAACGTCTCCTGAAAATGTTCCTCCAGGGACAATCATTGACAACAAAATATGTCACCCAAAGAACAATGATTTCTACCTCTGTGCTCACGCTGGAATGATTGGAACTACCCGCCCAACTCACTACCACGTCCTGTATGATGAGATTGGTTTTTCAGCTGACGAACTTCAGGAACTTGTCCACTCGCTCTCCTATGTGTACCAAAGAAGCACCAGTGCCATTTCTGTTGTTGCGCCGATCTGCTATGCTCACTTGGCAGCTGCTCAGCTTGGGACGTTCATGAAGTTTGAAGATCAGTCTGAGACATCATCAAGCCATGGTGGTATCACAGCTCCAGGACCAATCTCTGTTGCACAGCTCCCAAGACTCAAAGACAACGTCGCCAACTCCATGTTCTTCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003676 GO:0003682 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009553 GO:0009554 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009791 GO:0009814 GO:0009816 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010528 GO:0010529 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015030 GO:0016032 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016604 GO:0016787 GO:0016788 GO:0018022 GO:0018193 GO:0018205 GO:0019048 GO:0019219 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022414 GO:0030154 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0034293 GO:0034641 GO:0034968 GO:0035194 GO:0035197 GO:0035198 GO:0035670 GO:0035821 GO:0036211 GO:0040029 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0043934 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044703 GO:0044728 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048519 GO:0048523 GO:0048533 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0051567 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0060255 GO:0061458 GO:0061647 GO:0061980 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0090567 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901564 GO:1901698 GO:1901699 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:1904159 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

924

Amino Acids

102.84

Weight (kDa)

9.05

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 67 - 228 3e-31 N-terminal domain of argonaute
ArgoL1 PF08699 240 - 289 3.8e-16 Argonaute linker 1 domain
PAZ PF02170 294 - 424 1.3e-29 PAZ domain
ArgoL2 PF16488 434 - 479 1.8e-13 Argonaute linker 2 domain
ArgoMid PF16487 488 - 539 4.8e-07 Mid domain of argonaute
Piwi PF02171 577 - 885 3.5e-112 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1939
AasI GACNNNNNNGTC 1 cut(s) 1315
Acc36I ACCTGC 1 cut(s) 1939
AccB7I CCANNNNNTGG 2 cut(s) 524, 767
AccII CGCG 3 cut(s) 1116, 1214, 1837
AciI CCGC 8 cut(s) 590, 628, 1114, 1148, 1349, 1654, 1837, 2482
AclI AACGTT 1 cut(s) 1037
AclWI GGATC 6 cut(s) 199, 737, 1359, 1456, 1603, 1999
AcoI YGGCCR 2 cut(s) 591, 2048
AcsI RAATTY 6 cut(s) 597, 646, 992, 1496, 1515, 1802
AcuI CTGAAG 6 cut(s) 233, 1110, 1211, 1895, 2157, 2522
AfaI GTAC 4 cut(s) 1235, 1560, 1927, 2570
AfiI CCNNNNNNNGG 7 cut(s) 340, 524, 767, 1302, 1469, 1975, 2332
AflII CTTAAG 1 cut(s) 953
AflIII ACRYGT 1 cut(s) 1686
AgeI ACCGGT 1 cut(s) 555
AjiI CACGTC 1 cut(s) 2500
AjnI CCWGG 6 cut(s) 651, 868, 1714, 1996, 2395, 2704
AjuI GAANNNNNNNTTGG 1 cut(s) 2747
AleI CACNNNNGTG 1 cut(s) 270
Alw21I GWGCWC 4 cut(s) 1209, 1663, 1746, 2458
Alw26I GTCTC 2 cut(s) 2382, 2666
AlwI GGATC 6 cut(s) 199, 737, 1359, 1456, 1603, 1999
AlwNI CAGNNNCTG 2 cut(s) 1103, 2257
Ama87I CYCGRG 1 cut(s) 1202
AoxI GGCC 7 cut(s) 152, 196, 548, 591, 985, 1897, 2048
ApeKI GCWGC 4 cut(s) 695, 2024, 2627, 2630
ApoI RAATTY 6 cut(s) 597, 646, 992, 1496, 1515, 1802
ArsI GACNNNNNNTTYG 2 cut(s) 2733, 2765
AseI ATTAAT 1 cut(s) 1881
AsiGI ACCGGT 1 cut(s) 555
Asp700I GAANNNNTTC 3 cut(s) 117, 1458, 2388
AspLEI GCGC 2 cut(s) 1214, 2605
AspS9I GGNCC 7 cut(s) 152, 196, 558, 872, 913, 1775, 2708
AsuHPI GGTGA 4 cut(s) 407, 539, 2044, 2417
AsuII TTCGAA 1 cut(s) 1080
AvaI CYCGRG 1 cut(s) 1202
AvaII GGWCC 5 cut(s) 558, 872, 913, 1775, 2708
AxyI CCTNAGG 2 cut(s) 962, 982
BalI TGGCCA 1 cut(s) 2050
BanII GRGCYC 2 cut(s) 1209, 1509
BbsI GAAGAC 2 cut(s) 407, 2145
Bbv12I GWGCWC 4 cut(s) 1209, 1663, 1746, 2458
BbvI GCAGC 4 cut(s) 682, 2011, 2617, 2639
BccI CCATC 9 cut(s) 287, 365, 433, 518, 1518, 1969, 2027, 2138, 2219
BceAI ACGGC 1 cut(s) 1641
BciT130I CCWGG 6 cut(s) 653, 870, 1716, 1998, 2397, 2706
BclI TGATCA 3 cut(s) 858, 1849, 2272
BcoDI GTCTC 2 cut(s) 2382, 2666
BfaI CTAG 4 cut(s) 429, 699, 909, 2040
BfmI CTRYAG 1 cut(s) 268
BfrI CTTAAG 1 cut(s) 953
BfuAI ACCTGC 1 cut(s) 1939
BglI GCCNNNNNGGC 1 cut(s) 984
BglII AGATCT 1 cut(s) 1262
BisI GCNGC 6 cut(s) 591, 696, 1838, 2025, 2628, 2631
BlpI GCTNAGC 1 cut(s) 2634
BlsI GCNGC 6 cut(s) 592, 697, 1839, 2026, 2629, 2632
Bme1390I CCNGG 6 cut(s) 653, 870, 1716, 1998, 2397, 2706
Bme18I GGWCC 5 cut(s) 558, 872, 913, 1775, 2708
BmeT110I CYCGRG 1 cut(s) 1202
BmgBI CACGTC 1 cut(s) 2500
BmgT120I GGNCC 7 cut(s) 152, 196, 558, 872, 913, 1775, 2708
BmrFI CCNGG 6 cut(s) 653, 870, 1716, 1998, 2397, 2706
BmsI GCATC 4 cut(s) 355, 1170, 1336, 2081
BpiI GAAGAC 2 cut(s) 407, 2145
BplI GAGNNNNNCTC 2 cut(s) 2224, 2256
BpmI CTGGAG 3 cut(s) 784, 2379, 2688
Bpu1102I GCTNAGC 1 cut(s) 2634
Bpu14I TTCGAA 1 cut(s) 1080
BpuEI CTTGAG 1 cut(s) 479
BsaBI GATNNNNATC 2 cut(s) 1447, 1854
BsaJI CCNNGG 6 cut(s) 651, 1714, 1778, 1969, 2396, 2686
BsaWI WCCGGW 2 cut(s) 555, 1004
BsaXI ACNNNNNCTCC 2 cut(s) 2543, 2573
Bsc4I CCNNNNNNNGG 7 cut(s) 340, 524, 767, 1302, 1469, 1975, 2332
Bse118I RCCGGY 1 cut(s) 555
Bse1I ACTGG 8 cut(s) 92, 116, 155, 761, 801, 875, 935, 2583
Bse21I CCTNAGG 2 cut(s) 962, 982
Bse3DI GCAATG 1 cut(s) 630
Bse8I GATNNNNATC 2 cut(s) 1447, 1854
BseBI CCWGG 6 cut(s) 653, 870, 1716, 1998, 2397, 2706
BseDI CCNNGG 6 cut(s) 651, 1714, 1778, 1969, 2396, 2686
BseGI GGATG 9 cut(s) 376, 788, 847, 1311, 1448, 1698, 1980, 1984, 2230
BseJI GATNNNNATC 2 cut(s) 1447, 1854
BseLI CCNNNNNNNGG 7 cut(s) 340, 524, 767, 1302, 1469, 1975, 2332
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 7 cut(s) 60, 132, 996, 1412, 2091, 2648, 2661
BseNI ACTGG 8 cut(s) 92, 116, 155, 761, 801, 875, 935, 2583
BseRI GAGGAG 3 cut(s) 75, 1652, 1653
BseXI GCAGC 4 cut(s) 682, 2011, 2617, 2639
Bsh1236I CGCG 3 cut(s) 1116, 1214, 1837
BshFI GGCC 7 cut(s) 154, 198, 550, 593, 987, 1899, 2050
BshTI ACCGGT 1 cut(s) 555
BsiHKAI GWGCWC 4 cut(s) 1209, 1663, 1746, 2458
BsiHKCI CYCGRG 1 cut(s) 1202
BsiSI CCGG 2 cut(s) 556, 1005
BslFI GGGAC 7 cut(s) 645, 926, 1706, 1997, 2098, 2413, 2656
BslI CCNNNNNNNGG 7 cut(s) 340, 524, 767, 1302, 1469, 1975, 2332
BsmAI GTCTC 2 cut(s) 2382, 2666
BsmBI CGTCTC 1 cut(s) 2382
BsmFI GGGAC 7 cut(s) 645, 926, 1706, 1997, 2098, 2413, 2656
BsnI GGCC 7 cut(s) 154, 198, 550, 593, 987, 1899, 2050
BsoBI CYCGRG 1 cut(s) 1202
Bsp119I TTCGAA 1 cut(s) 1080
Bsp1286I GDGCHC 5 cut(s) 1209, 1509, 1663, 1746, 2458
Bsp1720I GCTNAGC 1 cut(s) 2634
Bsp19I CCATGG 2 cut(s) 1778, 2686
BspACI CCGC 8 cut(s) 590, 628, 1114, 1148, 1349, 1654, 1837, 2482
BspANI GGCC 7 cut(s) 154, 198, 550, 593, 987, 1899, 2050
BspCNI CTCAG 7 cut(s) 61, 133, 995, 1411, 2092, 2647, 2662
BspFNI CGCG 3 cut(s) 1116, 1214, 1837
BspHI TCATGA 1 cut(s) 2649
BspMI ACCTGC 1 cut(s) 1939
BspPI GGATC 6 cut(s) 199, 737, 1359, 1456, 1603, 1999
BspT104I TTCGAA 1 cut(s) 1080
BspTI CTTAAG 1 cut(s) 953
BsrDI GCAATG 1 cut(s) 630
BsrFI RCCGGY 1 cut(s) 555
BsrI ACTGG 8 cut(s) 92, 116, 155, 761, 801, 875, 935, 2583
BssAI RCCGGY 1 cut(s) 555
BssECI CCNNGG 6 cut(s) 651, 1714, 1778, 1969, 2396, 2686
BssT1I CCWWGG 3 cut(s) 1778, 1969, 2686
Bst2UI CCWGG 6 cut(s) 653, 870, 1716, 1998, 2397, 2706
Bst4CI ACNGT 9 cut(s) 272, 395, 726, 755, 1099, 1312, 1763, 1942, 2004
Bst6I CTCTTC 1 cut(s) 609
BstAFI CTTAAG 1 cut(s) 953
BstBI TTCGAA 1 cut(s) 1080
BstC8I GCNNGC 4 cut(s) 713, 1549, 2290, 2294
BstDSI CCRYGG 2 cut(s) 1778, 2686
BstENI CCTNNNNNAGG 1 cut(s) 338
BstF5I GGATG 9 cut(s) 376, 788, 847, 1311, 1448, 1698, 1980, 1984, 2230
BstFNI CGCG 3 cut(s) 1116, 1214, 1837
BstHHI GCGC 2 cut(s) 1214, 2605
BstMAI GTCTC 2 cut(s) 2382, 2666
BstMWI GCNNNNNNNGC 4 cut(s) 47, 479, 984, 2624
BstNI CCWGG 6 cut(s) 653, 870, 1716, 1998, 2397, 2706
BstNSI RCATGY 3 cut(s) 695, 829, 1690
BstSCI CCNGG 6 cut(s) 651, 868, 1714, 1996, 2395, 2704
BstSFI CTRYAG 1 cut(s) 268
BstUI CGCG 3 cut(s) 1116, 1214, 1837
BstV1I GCAGC 4 cut(s) 682, 2011, 2617, 2639
BstV2I GAAGAC 2 cut(s) 407, 2145
BstX2I RGATCY 4 cut(s) 204, 1262, 1448, 1991
BstYI RGATCY 4 cut(s) 204, 1262, 1448, 1991
Bsu36I CCTNAGG 2 cut(s) 962, 982
BsuRI GGCC 7 cut(s) 154, 198, 550, 593, 987, 1899, 2050
BtgI CCRYGG 2 cut(s) 1778, 2686
BtgZI GCGATG 1 cut(s) 2005
BtrI CACGTC 1 cut(s) 2500
BtsCI GGATG 9 cut(s) 376, 788, 847, 1311, 1448, 1698, 1980, 1984, 2230
BtsIMutI CAGTG 6 cut(s) 277, 882, 1768, 1938, 2051, 2590
BveI ACCTGC 1 cut(s) 1939
Cac8I GCNNGC 4 cut(s) 713, 1549, 2290, 2294
CaiI CAGNNNCTG 2 cut(s) 1103, 2257
CciI TCATGA 1 cut(s) 2649
CfoI GCGC 2 cut(s) 1214, 2605
Cfr10I RCCGGY 1 cut(s) 555
Cfr13I GGNCC 7 cut(s) 152, 196, 558, 872, 913, 1775, 2708
CseI GACGC 1 cut(s) 2312
Csp6I GTAC 4 cut(s) 1234, 1559, 1926, 2569
CspAI ACCGGT 1 cut(s) 555
CviQI GTAC 4 cut(s) 1234, 1559, 1926, 2569
DrdI GACNNNNNNGTC 1 cut(s) 1315
DseDI GACNNNNNNGTC 1 cut(s) 1315
EaeI YGGCCR 2 cut(s) 591, 2048
Eam1104I CTCTTC 1 cut(s) 609
EarI CTCTTC 1 cut(s) 609
EciI GGCGGA 1 cut(s) 1643
Ecl136II GAGCTC 1 cut(s) 1207
Eco130I CCWWGG 3 cut(s) 1778, 1969, 2686
Eco147I AGGCCT 2 cut(s) 550, 1899
Eco24I GRGCYC 2 cut(s) 1209, 1509
Eco32I GATATC 2 cut(s) 676, 2058
Eco47I GGWCC 5 cut(s) 558, 872, 913, 1775, 2708
Eco53kI GAGCTC 1 cut(s) 1207
Eco57I CTGAAG 6 cut(s) 233, 1110, 1211, 1895, 2157, 2522
Eco81I CCTNAGG 2 cut(s) 962, 982
Eco88I CYCGRG 1 cut(s) 1202
EcoICRI GAGCTC 1 cut(s) 1207
EcoNI CCTNNNNNAGG 1 cut(s) 338
EcoO109I RGGNCCY 1 cut(s) 913
EcoRI GAATTC 2 cut(s) 646, 992
EcoRII CCWGG 6 cut(s) 651, 868, 1714, 1996, 2395, 2704
EcoRV GATATC 2 cut(s) 676, 2058
EcoT14I CCWWGG 3 cut(s) 1778, 1969, 2686
EcoT22I ATGCAT 1 cut(s) 1826
EcoT38I GRGCYC 2 cut(s) 1209, 1509
ErhI CCWWGG 3 cut(s) 1778, 1969, 2686
Esp3I CGTCTC 1 cut(s) 2382
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FaqI GGGAC 7 cut(s) 645, 926, 1706, 1997, 2098, 2413, 2656
FauI CCCGC 2 cut(s) 621, 2489
FauNDI CATATG 1 cut(s) 415
FbaI TGATCA 3 cut(s) 858, 1849, 2272
Fnu4HI GCNGC 6 cut(s) 591, 696, 1838, 2025, 2628, 2631
FokI GGATG 9 cut(s) 383, 795, 854, 1318, 1455, 1685, 1987, 1991, 2237
FriOI GRGCYC 2 cut(s) 1209, 1509
Fsp4HI GCNGC 6 cut(s) 591, 696, 1838, 2025, 2628, 2631
FspBI CTAG 4 cut(s) 429, 699, 909, 2040
GlaI GCGC 2 cut(s) 1213, 2604
GluI GCNGC 6 cut(s) 591, 696, 1838, 2025, 2628, 2631
GsuI CTGGAG 3 cut(s) 784, 2379, 2688
HaeIII GGCC 7 cut(s) 154, 198, 550, 593, 987, 1899, 2050
HapII CCGG 2 cut(s) 556, 1005
HgaI GACGC 1 cut(s) 2312
HhaI GCGC 2 cut(s) 1214, 2605
Hin6I GCGC 2 cut(s) 1212, 2603
HinP1I GCGC 2 cut(s) 1212, 2603
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HindIII AAGCTT 1 cut(s) 1889
HinfI GANTC 8 cut(s) 5, 35, 638, 1672, 2111, 2237, 2347, 2736
HpaI GTTAAC 1 cut(s) 216
HpaII CCGG 2 cut(s) 556, 1005
HphI GGTGA 4 cut(s) 407, 539, 2044, 2417
Hpy166II GTNNAC 8 cut(s) 216, 411, 758, 1236, 1928, 1938, 2551, 2569
Hpy8I GTNNAC 8 cut(s) 216, 411, 758, 1236, 1928, 1938, 2551, 2569
Hpy99I CGWCG 1 cut(s) 2753
HpyAV CCTTC 2 cut(s) 142, 2100
HpyCH4III ACNGT 9 cut(s) 272, 395, 726, 755, 1099, 1312, 1763, 1942, 2004
HpyCH4IV ACGT 8 cut(s) 404, 1037, 1253, 1561, 2375, 2499, 2645, 2748
HpyCH4V TGCA 9 cut(s) 346, 623, 1026, 1138, 1161, 1824, 1886, 2292, 2723
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 479, 984, 2624
HpySE526I ACGT 8 cut(s) 404, 1037, 1253, 1561, 2375, 2499, 2645, 2748
HspAI GCGC 2 cut(s) 1212, 2603
KflI GGGWCCC 1 cut(s) 913
Ksp22I TGATCA 3 cut(s) 858, 1849, 2272
KspAI GTTAAC 1 cut(s) 216
LmnI GCTCC 9 cut(s) 23, 419, 1391, 1429, 1666, 1749, 1834, 2707, 2733
Lsp1109I GCAGC 4 cut(s) 682, 2011, 2617, 2639
LweI GCATC 4 cut(s) 355, 1170, 1336, 2081
MaeI CTAG 4 cut(s) 429, 699, 909, 2040
MaeII ACGT 8 cut(s) 404, 1037, 1253, 1561, 2375, 2499, 2645, 2748
MflI RGATCY 4 cut(s) 204, 1262, 1448, 1991
MhlI GDGCHC 5 cut(s) 1209, 1509, 1663, 1746, 2458
MlsI TGGCCA 1 cut(s) 2050
MluNI TGGCCA 1 cut(s) 2050
MlyI GAGTC 2 cut(s) 2120, 2730
MmeI TCCRAC 5 cut(s) 301, 770, 1413, 1856, 1982
Mox20I TGGCCA 1 cut(s) 2050
Mph1103I ATGCAT 1 cut(s) 1826
MroXI GAANNNNTTC 3 cut(s) 117, 1458, 2388
MscI TGGCCA 1 cut(s) 2050
MseI TTAA 9 cut(s) 126, 215, 834, 954, 1692, 1796, 1881, 1913, 2773
MslI CAYNNNNRTG 1 cut(s) 270
Msp20I TGGCCA 1 cut(s) 2050
MspA1I CMGCKG 2 cut(s) 2528, 2630
MspCI CTTAAG 1 cut(s) 953
MspI CCGG 2 cut(s) 556, 1005
MspR9I CCNGG 6 cut(s) 653, 870, 1716, 1998, 2397, 2706
MvaI CCWGG 6 cut(s) 653, 870, 1716, 1998, 2397, 2706
MvnI CGCG 3 cut(s) 1116, 1214, 1837
MwoI GCNNNNNNNGC 4 cut(s) 47, 479, 984, 2624
NcoI CCATGG 2 cut(s) 1778, 2686
NdeI CATATG 1 cut(s) 415
NmuCI GTSAC 3 cut(s) 828, 1013, 2423
NsiI ATGCAT 1 cut(s) 1826
NspI RCATGY 3 cut(s) 695, 829, 1690
NspV TTCGAA 1 cut(s) 1080
OliI CACNNNNGTG 1 cut(s) 270
PaeR7I CTCGAG 1 cut(s) 1202
PagI TCATGA 1 cut(s) 2649
PaqCI CACCTGC 1 cut(s) 1939
PceI AGGCCT 2 cut(s) 550, 1899
PciI ACATGT 1 cut(s) 1686
PdmI GAANNNNTTC 3 cut(s) 117, 1458, 2388
PfeI GAWTC 6 cut(s) 5, 35, 638, 1672, 2237, 2347
PflFI GACNNNGTC 1 cut(s) 2747
PflMI CCANNNNNTGG 2 cut(s) 524, 767
PfoI TCCNGGA 2 cut(s) 1996, 2704
PinAI ACCGGT 1 cut(s) 555
PkrI GCNGC 6 cut(s) 592, 697, 1839, 2026, 2629, 2632
PleI GAGTC 2 cut(s) 2119, 2730
PpsI GAGTC 2 cut(s) 2119, 2730
PpuMI RGGWCCY 1 cut(s) 913
PscI ACATGT 1 cut(s) 1686
PshBI ATTAAT 1 cut(s) 1881
Psp124BI GAGCTC 1 cut(s) 1209
Psp1406I AACGTT 1 cut(s) 1037
Psp5II RGGWCCY 1 cut(s) 913
Psp6I CCWGG 6 cut(s) 651, 868, 1714, 1996, 2395, 2704
PspGI CCWGG 6 cut(s) 651, 868, 1714, 1996, 2395, 2704
PspPI GGNCC 7 cut(s) 152, 196, 558, 872, 913, 1775, 2708
PspPPI RGGWCCY 1 cut(s) 913
PstNI CAGNNNCTG 2 cut(s) 1103, 2257
PsuI RGATCY 4 cut(s) 204, 1262, 1448, 1991
PsyI GACNNNGTC 1 cut(s) 2747
PvuII CAGCTG 2 cut(s) 2528, 2630
RsaI GTAC 4 cut(s) 1235, 1560, 1927, 2570
RsaNI GTAC 4 cut(s) 1234, 1559, 1926, 2569
RseI CAYNNNNRTG 1 cut(s) 270
SacI GAGCTC 1 cut(s) 1209
SaqAI TTAA 9 cut(s) 126, 215, 834, 954, 1692, 1796, 1881, 1913, 2773
SatI GCNGC 6 cut(s) 591, 696, 1838, 2025, 2628, 2631
Sau96I GGNCC 7 cut(s) 152, 196, 558, 872, 913, 1775, 2708
SchI GAGTC 2 cut(s) 2120, 2730
ScrFI CCNGG 6 cut(s) 653, 870, 1716, 1998, 2397, 2706
SduI GDGCHC 5 cut(s) 1209, 1509, 1663, 1746, 2458
SfaNI GCATC 4 cut(s) 355, 1170, 1336, 2081
SfcI CTRYAG 1 cut(s) 268
Sfr274I CTCGAG 1 cut(s) 1202
SfuI TTCGAA 1 cut(s) 1080
SinI GGWCC 5 cut(s) 558, 872, 913, 1775, 2708
SlaI CTCGAG 1 cut(s) 1202
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 3 cut(s) 458, 953, 1202
SmoI CTYRAG 3 cut(s) 458, 953, 1202
SseBI AGGCCT 2 cut(s) 550, 1899
SsiI CCGC 8 cut(s) 590, 628, 1114, 1148, 1349, 1654, 1837, 2482
SspI AATATT 2 cut(s) 1142, 2263
SspMI CTAG 4 cut(s) 429, 699, 909, 2040
SstI GAGCTC 1 cut(s) 1209
StuI AGGCCT 2 cut(s) 550, 1899
StyD4I CCNGG 6 cut(s) 651, 868, 1714, 1996, 2395, 2704
StyI CCWWGG 3 cut(s) 1778, 1969, 2686
TaaI ACNGT 9 cut(s) 272, 395, 726, 755, 1099, 1312, 1763, 1942, 2004
TaiI ACGT 8 cut(s) 407, 1040, 1256, 1564, 2378, 2502, 2648, 2751
TauI GCSGC 2 cut(s) 593, 1840
TfiI GAWTC 6 cut(s) 5, 35, 638, 1672, 2237, 2347
Tru1I TTAA 9 cut(s) 126, 215, 834, 954, 1692, 1796, 1881, 1913, 2773
Tru9I TTAA 9 cut(s) 126, 215, 834, 954, 1692, 1796, 1881, 1913, 2773
TscAI CASTG 6 cut(s) 277, 882, 1768, 1945, 2058, 2590
TseFI GTSAC 3 cut(s) 828, 1013, 2423
TseI GCWGC 4 cut(s) 695, 2024, 2627, 2630
Tsp45I GTSAC 3 cut(s) 828, 1013, 2423
TspDTI ATGAA 5 cut(s) 525, 1080, 1720, 2638, 2666
TspGWI ACGGA 2 cut(s) 36, 507
TspRI CASTG 6 cut(s) 277, 882, 1768, 1945, 2058, 2590
Tth111I GACNNNGTC 1 cut(s) 2747
Van91I CCANNNNNTGG 2 cut(s) 524, 767
Vha464I CTTAAG 1 cut(s) 953
VpaK11BI GGWCC 5 cut(s) 558, 872, 913, 1775, 2708
VspI ATTAAT 1 cut(s) 1881
XagI CCTNNNNNAGG 1 cut(s) 338
XapI RAATTY 6 cut(s) 597, 646, 992, 1496, 1515, 1802
XceI RCATGY 3 cut(s) 695, 829, 1690
XcmI CCANNNNNNNNNTGG 2 cut(s) 1435, 2026
XhoI CTCGAG 1 cut(s) 1202
XmnI GAANNNNTTC 3 cut(s) 117, 1458, 2388
XspI CTAG 4 cut(s) 429, 699, 909, 2040
Zsp2I ATGCAT 1 cut(s) 1826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.