RLG00000034661

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006994
Physical Location & Seq
Reverse (-)
47392829 .. 47401378
8550 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034661

Sequence Viewer

Length: 2172 bp
ATGGAAGACAACATCGCTCATTTGAGGGAAAAATCTCCAACATTCATTGGTCTTCCATTTGTGCTTGTCCCCGATCGTAACGGTGTTGCATCCCACTCCGAGATTCGAATTTTGAACTCGTTAAGTGACAGTGACCCACGCCGGTCCCCCACAACCAACAAATCTCATCCTTTCACGAGCTCTGCAAATTACCACACTACCCATTTCCAAGACCTTCTCATTGGAGACTTGGTAGTAATTCCAGAGTTTGGGCGGTGGCAAAACCGTCAAGAAGTTTCTGTTACGTATGAAGATGGTCGTCCCCTTGACGGCAAGGGTGCTGGAAGAAGGATAATTGAGAGAGTCCACCAGACTTATATCTCTGAGTTAGGTGGAAAGGACTTTGCTTATGATGGGGAGAAGAGTCTGTTTACTTCTTTCTTCCATAATGATCCAAAAAGTTTTGTTGATGTTGGAGGAGGTGTTCTAGGGTGCAGAGGATTCCATTCAAGCTTCAGAACCACCCAGGGTGGCCTGTCGTTGAACATTGATGTATCAACTACCATGATAATACAGCCTGGACCAGTTGTTGATTTTCTAATTTCCAACCAGAATGTTAGAGATCCCTTTTCACTTGACTGGACAAAGGACGGGGAGGATGGAGAAATTGAAGTGACTGTTTATGACTATTTTGTTACTCATCGCAATATACAGTTGCGTTATTCTGGCGATCTGCCCTGCATTAATGTTGGGAAGCCAAAGCGTCCTACTTACATTCCCCTTGAGCTATGTGAATTGGTTTCGTTGCAACGGTATACAAAAGCACTTTCAACTCTTCAAAGATCTTCACTGGTGGAGAAGTCGAGGCAAAAGCCACAGGAGAGGATGAGCATTTTGTCTAATGCTTTGAAAATCAACAATTATGATGGTGAACCTATGCTACGTGCATGTGGTGTATCAATTGGTAATAGTTTCACTCAAGTGGATGGCCGTGTTTTGCCTGCTCCAAAGTTAAAAGTTGGCAATGGGGATGATTTCTTCCCCAGAAATGGGCGGTGGAATTTCAACAATAAGAAACTCGTGAAGCCTACTAAGATAGAACGATGGGTTGTTGTTAACTTCTCCGCACGCTGTGATCTGAAAGCCCTTGTCCGTGATTTGATTAAATGTGGAGATATGAAGGGAATTGTAAGCCCATGGAAGCGGAAGAATCTGGCTGAGTATGGTATTGTCACACAGTGCATTGCGCCTACCAGGGTCAATGATCAATACCTTACAAATGTTCTGCTAAAAATTAATGCTAAGCTTGGTGGATTAAATTCCTTGTTGGCTGTTGAGTTTTCTCCTTCCATTCCTGTGGTTTCCAAGGCTCCTACCATCATCCTAGGGATGGATGTGTCACATGGCTCTCCTGGCCAGTCGGACATACCATCAATAGCCGCGGTAGTCAGCTCGAGGCAGTGGCCGCTCATTTCCCGCTATAGGGCATCTGTTCGAACTCAGTCTCCAAAGGTCGAAATGATTGATTCACTGTTCAAGCGTGTTTCTGATACAGAAGATGAAGGCATTATGAGAGAGTTGCTTTTGGACTTCTATACAAGTTCAAATAAGCGTAAACCGGATCAGATAATCATATTCAGGGATGGTGTCAGCGAGTCTCAATTCAACCAAGTCTTGAACATTGAACTGGATCAAATCATTGAGGCGTGCAAGTTCCTTGACGAGAGTTGGTGCCCAAAATTTGTGGTGATTATTGCTCAGAAAAACCACCATACAAAATTCTTTCAGCCTCAGTCACCTGATAATGTTCCACCTGGTACCATTATTGACAACAGAATTTGTCATCCAAAGAACAATGATTTTTATCTCTGTGCACAGGCCGGAATGATAGGTACCACGAGACCTACTCATTATCATGTTCTGTTGGACGACGTTGGGTTTTCAGCTGATGATCTGCAGGAACTAGTGCATTCTCTGTCTTATGTGTACCAGAGAAGTACCACTGCCATTTCTGTAGTTGCTCCAATTTGCTATGCTCATCTCGCTGCCACTCAAATGAGTCAGTTTGTGAAATTTGAAGATGCATCTGAGACTTCCTCAAGCCATGGTGGGTTGACCTCTGCTGGAGCAGTGCCCGTCCCACAGCTTCCCAGGCTCAAGGAGAACGTGGCCAATTCCATGTTTTTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003682 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0008150 GO:0008152 GO:0008213 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009814 GO:0009816 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015030 GO:0016032 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016604 GO:0016787 GO:0016788 GO:0018022 GO:0018193 GO:0018205 GO:0019048 GO:0019219 GO:0019222 GO:0019538 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0034641 GO:0034968 GO:0035194 GO:0035197 GO:0035198 GO:0035821 GO:0036211 GO:0040029 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044728 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0060255 GO:0061647 GO:0061980 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0140098 GO:1901360 GO:1901363 GO:1901564 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

724

Amino Acids

80.75

Weight (kDa)

7.88

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoL1 PF08699 139 - 185 2.2e-17 Argonaute linker 1 domain
PAZ PF02170 193 - 277 6.8e-18 PAZ domain
ArgoL2 PF16488 287 - 332 8.3e-13 Argonaute linker 2 domain
Piwi PF02171 388 - 684 1.5e-101 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1796, 1871
AccB1I GGYRCC 3 cut(s) 1710, 1796, 1871
AccB7I CCANNNNNTGG 1 cut(s) 248
AccBSI CCGCTC 1 cut(s) 1447
AccI GTMKAC 1 cut(s) 794
AccII CGCG 1 cut(s) 1421
AciI CCGC 8 cut(s) 253, 1033, 1104, 1183, 1419, 1421, 1445, 1456
AclWI GGATC 4 cut(s) 425, 596, 1608, 1677
AcoI YGGCCR 4 cut(s) 967, 1393, 1442, 2148
AcsI RAATTY 7 cut(s) 108, 1039, 1297, 1718, 1757, 1815, 2051
AcuI CTGAAG 1 cut(s) 478
AdeI CACNNNGTG 2 cut(s) 1112, 1218
AfaI GTAC 4 cut(s) 1798, 1873, 1967, 1978
AfiI CCNNNNNNNGG 7 cut(s) 248, 308, 1028, 1029, 1369, 1461, 1462
AhlI ACTAGT 1 cut(s) 1942
AjnI CCWGG 6 cut(s) 504, 556, 1232, 1390, 1792, 2129
AleI CACNNNNGTG 1 cut(s) 959
AloI GAACNNNNNNTCC 4 cut(s) 447, 479, 1468, 1500
AluBI AGCT 7 cut(s) 180, 492, 766, 1285, 1431, 1925, 2125
AluI AGCT 7 cut(s) 180, 492, 766, 1285, 1431, 1925, 2125
Alw21I GWGCWC 2 cut(s) 182, 1855
Alw26I GTCTC 5 cut(s) 219, 1488, 1641, 1873, 2063
Alw44I GTGCAC 1 cut(s) 1851
AlwI GGATC 4 cut(s) 425, 596, 1608, 1677
Ama87I CYCGRG 1 cut(s) 1432
AoxI GGCC 6 cut(s) 511, 967, 1393, 1442, 1857, 2148
ApaLI GTGCAC 1 cut(s) 1851
ApeKI GCWGC 1 cut(s) 2024
ApoI RAATTY 7 cut(s) 108, 1039, 1297, 1718, 1757, 1815, 2051
AseI ATTAAT 2 cut(s) 723, 1275
Asp718I GGTACC 2 cut(s) 1796, 1871
AspA2I CCTAGG 1 cut(s) 1363
AspLEI GCGC 1 cut(s) 1228
AspS9I GGNCC 2 cut(s) 144, 560
AsuHPI GGTGA 3 cut(s) 920, 1738, 1767
AsuII TTCGAA 2 cut(s) 106, 1474
AvaI CYCGRG 1 cut(s) 1432
AvaII GGWCC 2 cut(s) 144, 560
AvrII CCTAGG 1 cut(s) 1363
BaeGI GKGCMC 3 cut(s) 1715, 1855, 2115
BalI TGGCCA 2 cut(s) 1395, 2150
BanI GGYRCC 3 cut(s) 1710, 1796, 1871
BanII GRGCYC 1 cut(s) 182
BauI CACGAG 3 cut(s) 175, 1058, 1876
BbsI GAAGAC 2 cut(s) 12, 44
Bbv12I GWGCWC 2 cut(s) 182, 1855
BbvI GCAGC 1 cut(s) 2011
BceAI ACGGC 2 cut(s) 325, 954
BciT130I CCWGG 6 cut(s) 506, 558, 1234, 1392, 1794, 2131
BclI TGATCA 1 cut(s) 1243
BcoDI GTCTC 5 cut(s) 219, 1488, 1641, 1873, 2063
BcuI ACTAGT 1 cut(s) 1942
BfaI CTAG 3 cut(s) 467, 1364, 1943
BfmI CTRYAG 3 cut(s) 1459, 1934, 1992
BglII AGATCT 1 cut(s) 821
BisI GCNGC 3 cut(s) 1419, 1445, 2025
BlnI CCTAGG 1 cut(s) 1363
BlpI GCTNAGC 1 cut(s) 1281
BlsI GCNGC 3 cut(s) 1420, 1446, 2026
Bme1390I CCNGG 6 cut(s) 506, 558, 1234, 1392, 1794, 2131
Bme18I GGWCC 2 cut(s) 144, 560
BmeT110I CYCGRG 1 cut(s) 1432
BmgT120I GGNCC 2 cut(s) 144, 560
BmiI GGNNCC 5 cut(s) 146, 1350, 1712, 1798, 1873
BmrFI CCNGG 6 cut(s) 506, 558, 1234, 1392, 1794, 2131
BmsI GCATC 4 cut(s) 98, 1475, 2050, 2072
BpiI GAAGAC 2 cut(s) 12, 44
BplI GAGNNNNNCTC 4 cut(s) 1870, 1902, 2060, 2092
BpmI CTGGAG 1 cut(s) 2124
Bpu1102I GCTNAGC 1 cut(s) 1281
Bpu14I TTCGAA 2 cut(s) 106, 1474
BpuEI CTTGAG 4 cut(s) 782, 942, 2062, 2120
BsaAI YACGTR 2 cut(s) 285, 923
BsaBI GATNNNNATC 1 cut(s) 1842
BsaI GGTCTC 1 cut(s) 1873
BsaJI CCNNGG 9 cut(s) 504, 505, 1175, 1233, 1344, 1363, 1419, 2083, 2129
BsaWI WCCGGW 1 cut(s) 1597
BsaXI ACNNNNNCTCC 6 cut(s) 216, 246, 447, 477, 1468, 1498
Bsc4I CCNNNNNNNGG 7 cut(s) 248, 308, 1028, 1029, 1369, 1461, 1462
Bse118I RCCGGY 1 cut(s) 141
Bse1I ACTGG 5 cut(s) 563, 623, 834, 1396, 1671
Bse3DI GCAATG 2 cut(s) 1009, 1221
Bse8I GATNNNNATC 1 cut(s) 1842
BseBI CCWGG 6 cut(s) 506, 558, 1234, 1392, 1794, 2131
BseDI CCNNGG 9 cut(s) 504, 505, 1175, 1233, 1344, 1363, 1419, 2083, 2129
BseJI GATNNNNATC 1 cut(s) 1842
BseLI CCNNNNNNNGG 7 cut(s) 248, 308, 1028, 1029, 1369, 1461, 1462
BseMI GCAATG 2 cut(s) 1009, 1221
BseMII CTCAG 6 cut(s) 354, 1188, 1493, 1751, 1784, 2058
BseNI ACTGG 5 cut(s) 563, 623, 834, 1396, 1671
BseRI GAGGAG 1 cut(s) 471
BseSI GKGCMC 3 cut(s) 1715, 1855, 2115
BseXI GCAGC 1 cut(s) 2011
BsgI GTGCAG 1 cut(s) 493
Bsh1236I CGCG 1 cut(s) 1421
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 6 cut(s) 513, 969, 1395, 1444, 1859, 2150
BshNI GGYRCC 3 cut(s) 1710, 1796, 1871
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 2 cut(s) 182, 1855
BsiHKCI CYCGRG 1 cut(s) 1432
BsiSI CCGG 3 cut(s) 142, 1598, 1860
BslFI GGGAC 4 cut(s) 53, 130, 285, 2102
BslI CCNNNNNNNGG 7 cut(s) 248, 308, 1028, 1029, 1369, 1461, 1462
BsmAI GTCTC 5 cut(s) 219, 1488, 1641, 1873, 2063
BsmFI GGGAC 4 cut(s) 53, 130, 285, 2102
BsmI GAATGC 1 cut(s) 1948
BsnI GGCC 6 cut(s) 513, 969, 1395, 1444, 1859, 2150
Bso31I GGTCTC 1 cut(s) 1873
BsoBI CYCGRG 1 cut(s) 1432
Bsp119I TTCGAA 2 cut(s) 106, 1474
Bsp1286I GDGCHC 4 cut(s) 182, 1715, 1855, 2115
Bsp1720I GCTNAGC 1 cut(s) 1281
Bsp19I CCATGG 2 cut(s) 1175, 2083
BspACI CCGC 8 cut(s) 253, 1033, 1104, 1183, 1419, 1421, 1445, 1456
BspANI GGCC 6 cut(s) 513, 969, 1395, 1444, 1859, 2150
BspCNI CTCAG 6 cut(s) 355, 1189, 1492, 1750, 1783, 2059
BspFNI CGCG 1 cut(s) 1421
BspLI GGNNCC 5 cut(s) 146, 1350, 1712, 1798, 1873
BspMAI CTGCAG 1 cut(s) 1938
BspPI GGATC 4 cut(s) 425, 596, 1608, 1677
BspT104I TTCGAA 2 cut(s) 106, 1474
BspT107I GGYRCC 3 cut(s) 1710, 1796, 1871
BspTNI GGTCTC 1 cut(s) 1873
BsrBI CCGCTC 1 cut(s) 1447
BsrDI GCAATG 2 cut(s) 1009, 1221
BsrFI RCCGGY 1 cut(s) 141
BsrI ACTGG 5 cut(s) 563, 623, 834, 1396, 1671
BssAI RCCGGY 1 cut(s) 141
BssECI CCNNGG 9 cut(s) 504, 505, 1175, 1233, 1344, 1363, 1419, 2083, 2129
BssNAI GTATAC 1 cut(s) 795
BssSI CACGAG 3 cut(s) 175, 1058, 1876
BssT1I CCWWGG 4 cut(s) 1175, 1344, 1363, 2083
Bst1107I GTATAC 1 cut(s) 795
Bst2BI CACGAG 3 cut(s) 175, 1058, 1876
Bst2UI CCWGG 6 cut(s) 506, 558, 1234, 1392, 1794, 2131
Bst4CI ACNGT 8 cut(s) 83, 131, 266, 658, 693, 792, 1218, 1512
Bst6I CTCTTC 2 cut(s) 395, 819
BstBAI YACGTR 2 cut(s) 285, 923
BstBI TTCGAA 2 cut(s) 106, 1474
BstC8I GCNNGC 3 cut(s) 981, 1108, 1687
BstDEI CTNAG 8 cut(s) 363, 1071, 1197, 1281, 1479, 1737, 1770, 2067
BstDSI CCRYGG 3 cut(s) 1175, 1419, 2083
BstFNI CGCG 1 cut(s) 1421
BstHHI GCGC 1 cut(s) 1228
BstMAI GTCTC 5 cut(s) 219, 1488, 1641, 1873, 2063
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 4 cut(s) 1392, 1444, 2021, 2131
BstNI CCWGG 6 cut(s) 506, 558, 1234, 1392, 1794, 2131
BstNSI RCATGY 1 cut(s) 930
BstSCI CCNGG 6 cut(s) 504, 556, 1232, 1390, 1792, 2129
BstSFI CTRYAG 3 cut(s) 1459, 1934, 1992
BstSLI GKGCMC 3 cut(s) 1715, 1855, 2115
BstSNI TACGTA 1 cut(s) 285
BstUI CGCG 1 cut(s) 1421
BstV1I GCAGC 1 cut(s) 2011
BstV2I GAAGAC 2 cut(s) 12, 44
BstX2I RGATCY 2 cut(s) 601, 821
BstXI CCANNNNNNTGG 1 cut(s) 1336
BstYI RGATCY 2 cut(s) 601, 821
BstZ17I GTATAC 1 cut(s) 795
BsuRI GGCC 6 cut(s) 513, 969, 1395, 1444, 1859, 2150
BtgI CCRYGG 3 cut(s) 1175, 1419, 2083
BtgZI GCGATG 1 cut(s) 665
BtsI GCAGTG 3 cut(s) 1445, 1980, 2115
BtsIMutI CAGTG 7 cut(s) 136, 827, 1223, 1445, 1508, 1980, 2115
Cac8I GCNNGC 3 cut(s) 981, 1108, 1687
CfoI GCGC 1 cut(s) 1228
Cfr10I RCCGGY 1 cut(s) 141
Cfr13I GGNCC 2 cut(s) 144, 560
Cfr42I CCGCGG 1 cut(s) 1422
CseI GACGC 1 cut(s) 731
CsiI ACCWGGT 1 cut(s) 1792
Csp6I GTAC 4 cut(s) 1797, 1872, 1966, 1977
CspCI CAANNNNNGTGG 2 cut(s) 1704, 1739
CviAII CATG 7 cut(s) 544, 927, 1176, 1382, 1895, 2084, 2158
CviQI GTAC 4 cut(s) 1797, 1872, 1966, 1977
DdeI CTNAG 8 cut(s) 363, 1071, 1197, 1281, 1479, 1737, 1770, 2067
DraIII CACNNNGTG 2 cut(s) 1112, 1218
EaeI YGGCCR 4 cut(s) 967, 1393, 1442, 2148
Eam1104I CTCTTC 2 cut(s) 395, 819
EarI CTCTTC 2 cut(s) 395, 819
Ecl136II GAGCTC 1 cut(s) 180
Eco105I TACGTA 1 cut(s) 285
Eco130I CCWWGG 4 cut(s) 1175, 1344, 1363, 2083
Eco24I GRGCYC 1 cut(s) 182
Eco31I GGTCTC 1 cut(s) 1873
Eco47I GGWCC 2 cut(s) 144, 560
Eco53kI GAGCTC 1 cut(s) 180
Eco57I CTGAAG 1 cut(s) 478
Eco88I CYCGRG 1 cut(s) 1432
EcoICRI GAGCTC 1 cut(s) 180
EcoRII CCWGG 6 cut(s) 504, 556, 1232, 1390, 1792, 2129
EcoT14I CCWWGG 4 cut(s) 1175, 1344, 1363, 2083
EcoT22I ATGCAT 1 cut(s) 2065
EcoT38I GRGCYC 1 cut(s) 182
ErhI CCWWGG 4 cut(s) 1175, 1344, 1363, 2083
FaeI CATG 7 cut(s) 547, 930, 1179, 1385, 1898, 2087, 2161
FaqI GGGAC 4 cut(s) 53, 130, 285, 2102
FatI CATG 7 cut(s) 543, 926, 1175, 1381, 1894, 2083, 2157
FauI CCCGC 1 cut(s) 1463
FbaI TGATCA 1 cut(s) 1243
FblI GTMKAC 1 cut(s) 794
Fnu4HI GCNGC 3 cut(s) 1419, 1445, 2025
FriOI GRGCYC 1 cut(s) 182
Fsp4HI GCNGC 3 cut(s) 1419, 1445, 2025
FspBI CTAG 3 cut(s) 467, 1364, 1943
GlaI GCGC 1 cut(s) 1227
GluI GCNGC 3 cut(s) 1419, 1445, 2025
GsuI CTGGAG 1 cut(s) 2124
HaeIII GGCC 6 cut(s) 513, 969, 1395, 1444, 1859, 2150
HapII CCGG 3 cut(s) 142, 1598, 1860
HgaI GACGC 1 cut(s) 731
HhaI GCGC 1 cut(s) 1228
Hin1II CATG 7 cut(s) 547, 930, 1179, 1385, 1898, 2087, 2161
Hin6I GCGC 1 cut(s) 1226
HinP1I GCGC 1 cut(s) 1226
HincII GTYRAC 2 cut(s) 1096, 2094
HindII GTYRAC 2 cut(s) 1096, 2094
HindIII AAGCTT 2 cut(s) 490, 1283
HinfI GANTC 8 cut(s) 103, 342, 403, 480, 1189, 1505, 1634, 2038
HpaI GTTAAC 1 cut(s) 1096
HpaII CCGG 3 cut(s) 142, 1598, 1860
HphI GGTGA 3 cut(s) 920, 1738, 1767
Hpy166II GTNNAC 9 cut(s) 346, 411, 795, 911, 1096, 1595, 1853, 1966, 2094
Hpy188I TCNGA 9 cut(s) 100, 364, 497, 1119, 1402, 1528, 1605, 1740, 2068
Hpy188III TCNNGA 5 cut(s) 175, 242, 269, 1060, 1654
Hpy8I GTNNAC 9 cut(s) 346, 411, 795, 911, 1096, 1595, 1853, 1966, 2094
Hpy99I CGWCG 1 cut(s) 1913
HpyAV CCTTC 5 cut(s) 224, 321, 1153, 1335, 1535
HpyCH4III ACNGT 8 cut(s) 83, 131, 266, 658, 693, 792, 1218, 1512
HpyCH4IV ACGT 4 cut(s) 284, 922, 1911, 2145
HpyF10VI GCNNNNNNNGC 4 cut(s) 1392, 1444, 2021, 2131
HpyF3I CTNAG 8 cut(s) 363, 1071, 1197, 1281, 1479, 1737, 1770, 2067
HpySE526I ACGT 4 cut(s) 284, 922, 1911, 2145
Hsp92II CATG 7 cut(s) 547, 930, 1179, 1385, 1898, 2087, 2161
HspAI GCGC 1 cut(s) 1226
KpnI GGTACC 2 cut(s) 1800, 1875
Ksp22I TGATCA 1 cut(s) 1243
KspAI GTTAAC 1 cut(s) 1096
KspI CCGCGG 1 cut(s) 1422
LmnI GCTCC 4 cut(s) 988, 1354, 2005, 2105
Lsp1109I GCAGC 1 cut(s) 2011
LweI GCATC 4 cut(s) 98, 1475, 2050, 2072
MabI ACCWGGT 1 cut(s) 1792
MaeI CTAG 3 cut(s) 467, 1364, 1943
MaeII ACGT 4 cut(s) 284, 922, 1911, 2145
MaeIII GTNAC 9 cut(s) 77, 125, 131, 280, 652, 673, 1210, 1377, 1773
MbiI CCGCTC 1 cut(s) 1447
MfeI CAATTG 1 cut(s) 939
MflI RGATCY 2 cut(s) 601, 821
MhlI GDGCHC 4 cut(s) 182, 1715, 1855, 2115
MlsI TGGCCA 2 cut(s) 1395, 2150
MluNI TGGCCA 2 cut(s) 1395, 2150
MlyI GAGTC 4 cut(s) 351, 412, 1643, 2047
MmeI TCCRAC 5 cut(s) 62, 433, 609, 1380, 1884
Mox20I TGGCCA 2 cut(s) 1395, 2150
Mph1103I ATGCAT 1 cut(s) 2065
MscI TGGCCA 2 cut(s) 1395, 2150
MseI TTAA 7 cut(s) 122, 723, 992, 1095, 1143, 1275, 1295
MslI CAYNNNNRTG 4 cut(s) 959, 1334, 1893, 2033
Msp20I TGGCCA 2 cut(s) 1395, 2150
MspA1I CMGCKG 2 cut(s) 1421, 1925
MspI CCGG 3 cut(s) 142, 1598, 1860
MspR9I CCNGG 6 cut(s) 506, 558, 1234, 1392, 1794, 2131
MunI CAATTG 1 cut(s) 939
Mva1269I GAATGC 1 cut(s) 1948
MvaI CCWGG 6 cut(s) 506, 558, 1234, 1392, 1794, 2131
MvnI CGCG 1 cut(s) 1421
MwoI GCNNNNNNNGC 4 cut(s) 1392, 1444, 2021, 2131
NcoI CCATGG 2 cut(s) 1175, 2083
NlaIII CATG 7 cut(s) 547, 930, 1179, 1385, 1898, 2087, 2161
NlaIV GGNNCC 5 cut(s) 146, 1350, 1712, 1798, 1873
NmuCI GTSAC 6 cut(s) 125, 131, 652, 1210, 1377, 1773
NsiI ATGCAT 1 cut(s) 2065
NspI RCATGY 1 cut(s) 930
NspV TTCGAA 2 cut(s) 106, 1474
OliI CACNNNNGTG 1 cut(s) 959
PaeR7I CTCGAG 1 cut(s) 1432
PasI CCCWGGG 1 cut(s) 505
PctI GAATGC 1 cut(s) 1948
PfeI GAWTC 4 cut(s) 103, 480, 1189, 1505
PflMI CCANNNNNTGG 1 cut(s) 248
PkrI GCNGC 3 cut(s) 1420, 1446, 2026
Ple19I CGATCG 1 cut(s) 76
PleI GAGTC 4 cut(s) 350, 411, 1642, 2046
PpsI GAGTC 4 cut(s) 350, 411, 1642, 2046
Ppu21I YACGTR 2 cut(s) 285, 923
PshBI ATTAAT 2 cut(s) 723, 1275
Psp124BI GAGCTC 1 cut(s) 182
Psp6I CCWGG 6 cut(s) 504, 556, 1232, 1390, 1792, 2129
PspGI CCWGG 6 cut(s) 504, 556, 1232, 1390, 1792, 2129
PspN4I GGNNCC 5 cut(s) 146, 1350, 1712, 1798, 1873
PspPI GGNCC 2 cut(s) 144, 560
PspXI VCTCGAGB 1 cut(s) 1432
PsrI GAACNNNNNNTAC 2 cut(s) 903, 935
PstI CTGCAG 1 cut(s) 1938
PsuI RGATCY 2 cut(s) 601, 821
PvuI CGATCG 1 cut(s) 76
PvuII CAGCTG 1 cut(s) 1925
RsaI GTAC 4 cut(s) 1798, 1873, 1967, 1978
RsaNI GTAC 4 cut(s) 1797, 1872, 1966, 1977
RseI CAYNNNNRTG 4 cut(s) 959, 1334, 1893, 2033
SacI GAGCTC 1 cut(s) 182
SacII CCGCGG 1 cut(s) 1422
SaqAI TTAA 7 cut(s) 122, 723, 992, 1095, 1143, 1275, 1295
SatI GCNGC 3 cut(s) 1419, 1445, 2025
Sau96I GGNCC 2 cut(s) 144, 560
SchI GAGTC 4 cut(s) 351, 412, 1643, 2047
ScrFI CCNGG 6 cut(s) 506, 558, 1234, 1392, 1794, 2131
SduI GDGCHC 4 cut(s) 182, 1715, 1855, 2115
SexAI ACCWGGT 1 cut(s) 1792
SfaNI GCATC 4 cut(s) 98, 1475, 2050, 2072
SfcI CTRYAG 3 cut(s) 1459, 1934, 1992
Sfr274I CTCGAG 1 cut(s) 1432
Sfr303I CCGCGG 1 cut(s) 1422
SfuI TTCGAA 2 cut(s) 106, 1474
SgrBI CCGCGG 1 cut(s) 1422
SinI GGWCC 2 cut(s) 144, 560
SlaI CTCGAG 1 cut(s) 1432
SmiMI CAYNNNNRTG 4 cut(s) 959, 1334, 1893, 2033
SmlI CTYRAG 5 cut(s) 761, 957, 1432, 2077, 2135
SmoI CTYRAG 5 cut(s) 761, 957, 1432, 2077, 2135
SnaBI TACGTA 1 cut(s) 285
SpeI ACTAGT 1 cut(s) 1942
SsiI CCGC 8 cut(s) 253, 1033, 1104, 1183, 1419, 1421, 1445, 1456
SspMI CTAG 3 cut(s) 467, 1364, 1943
SstI GAGCTC 1 cut(s) 182
StyD4I CCNGG 6 cut(s) 504, 556, 1232, 1390, 1792, 2129
StyI CCWWGG 4 cut(s) 1175, 1344, 1363, 2083
TaaI ACNGT 8 cut(s) 83, 131, 266, 658, 693, 792, 1218, 1512
TaiI ACGT 4 cut(s) 287, 925, 1914, 2148
TaqI TCGA 5 cut(s) 106, 842, 1433, 1474, 1494
TauI GCSGC 2 cut(s) 1421, 1447
TfiI GAWTC 4 cut(s) 103, 480, 1189, 1505
Tru1I TTAA 7 cut(s) 122, 723, 992, 1095, 1143, 1275, 1295
Tru9I TTAA 7 cut(s) 122, 723, 992, 1095, 1143, 1275, 1295
TscAI CASTG 7 cut(s) 136, 834, 1223, 1445, 1515, 1987, 2115
TseFI GTSAC 6 cut(s) 125, 131, 652, 1210, 1377, 1773
TseI GCWGC 1 cut(s) 2024
Tsp45I GTSAC 6 cut(s) 125, 131, 652, 1210, 1377, 1773
TspDTI ATGAA 4 cut(s) 34, 303, 1172, 1554
TspGWI ACGGA 1 cut(s) 1121
TspRI CASTG 7 cut(s) 136, 834, 1223, 1445, 1515, 1987, 2115
Van91I CCANNNNNTGG 1 cut(s) 248
VneI GTGCAC 1 cut(s) 1851
VpaK11BI GGWCC 2 cut(s) 144, 560
VspI ATTAAT 2 cut(s) 723, 1275
XapI RAATTY 7 cut(s) 108, 1039, 1297, 1718, 1757, 1815, 2051
XceI RCATGY 1 cut(s) 930
XhoI CTCGAG 1 cut(s) 1432
XmaJI CCTAGG 1 cut(s) 1363
XmiI GTMKAC 1 cut(s) 794
XspI CTAG 3 cut(s) 467, 1364, 1943
Zsp2I ATGCAT 1 cut(s) 2065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.