FvH4_3g27260

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006994
Physical Location & Seq
Forward (+)
20342488 .. 20350236
7749 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g27260.t2

Sequence Viewer

Length: 2631 bp
ATGGATTCATTTGAGCCAGATGGAAATGGGACAAATGTCCAAAATGGGGCAAATGGAGACCACGGGTCAAATGTTGAAAATGGGGCAACTGGAGAAAACGGGGCAAATGGAAGTGCGGCAGAACACGCATTGCCACCCCCACCCCCTGTTATTCCCCCAGATGTTGTTCCGTTGCGTGCAGAAATTGCACCTGAACCTGTAAAGAAGAAGAATTTACGCCTACCAATTGCTAGGCGAGGCCTTGGAACAAAAGGGCAAAAGATTCCTCTGTTAACTAATCATTTCAAAGTAAACGTCACTAATAGTAACAAAGAAGAACACTTCTTCCATTACAGTGTTCTGGTTACTTATGAAGACGGTCGTCCCCTTGATGGGAAGGGTGCTGGAAGAAGGATAATTGATCGAGTCCATGAGACATATAACTCCGAGTTAGGTGGAAAGGACTTCGCATATGATGGTGAGAAGAGTTTGTTCACTGTTGGTTCACTTCCACGCAACAAACTTGAATTTTCGGTTGTTCTGGAGGATACACCATCAAACAGAAATAATGGAAGTATTAATGCTGATGGTGAAGGGAGTCCAAATGAAAGTGATCGGAAGAGGCTACGACGTCCAGGTCGTACCAAAACATTCAATGTTGAAATTAGTTATGCTGCAAAAATTCCCATGAAGGCAATTGGAGAAGCTCTGCGGGGTCAGGAGTCCGAAAACTCCCAAGAAGCTCTGAGAGTGCTGGATATTATATTGAGGCAACATGCATCTAAGCAAGGTTGTCTCCTAGTGCGTCAATCTTTCTTCCATAATGACCCAAAAAGTTTTGTTGATGTCGGAGGAGGTGTTCTAGGGTGCAGAGGATTCCATTCAAGCTTCAGAACCACCCAGGGTGGCCTGTCGTTGAACATTGATGTATCAACCACCATGATCATACAGCCTGGACCAGTTGTTGATTTTTTAATTTCCAGCCAGAATGTTAGAGATCCCTTTTCACTTGACTGGACAAAGGCTAAGAGAACTCTGAAGAATTTGAGGGTTAAAACCAGCCCAACCAATCTAGAATACAAAATAACTGGATTGAGCGAGAGACCATGCAAAGAGCAATTGTTTACATTGAGAAACAAAATTGCAAAGGATGGTGAGGATGGAGAAATTGAAGTGACTGTGTACGACTATTTTGTTAATCATCGGAATATACCGTTGCGTTATTCTGGCGATCTGCCCTGCATTAATGTTGGGAAGCCCAAGCGTCCAACATACATCCCCCTTGAGCTATGTGAATTGGTTTCATTGCAACGGTATACAAAAGCACTTTCAACCCTTCAAAGATCTTCACTGGTGGAGAAATCAAGGCAAAAGCCACAGGAGAGGATGAGCATCTTGTCTAACGCTTTGAAAATCAACAATTATGATGGTGAACCTATGCTACGTGCATGTGGTGTTTCAATTGGTAATAGTTTTACACAAGTGGATGGCCGCGTTCTGCCTGCTCCTAAGTTGAAAGTCGGCAATGGGGATGATTTCTTTCCCAGAAATGGGCGGTGGAATTTCAACAATAAGAAACTTGTCAAGCCCACTAAGATAGAACGATGGGTTGTTGTTAACTTCTCCGCACGCTGTGATCTGAAAGCCCTTGTCCGTGATTTGATTAAATGTGGAGATATGAAGGGAATTGCCATAGACCCTCCATTTGACGTTTTTGAAGAGAATCCACAGTCGAGACGTGCCCCGCCTATGGTTAGGGTTGAGAGAATATTTGAGGATATTCAGTCCAAACTTCCTGGACAACCGCAGTTCATTCTCTGTTTGCTTCCGGAGAGGAAAAATTCTGCTCTATATGGTCCATGGAAGCGGAAGAATCTGGCCGAGTATGGCATTGTCACACAGTGCATAGCTCCTACAAGGGTCAATGACCAATACCTGACTAATGTTTTGCTAAAAATTAATGCGAAGCTTGGAGGATTGAATTCCTTGTTGGCTGTTGAATTTTCACCTTCCATTCCTGTGGTTTCCAAGGCTCCTACCATCATCCTAGGAATGGATGTGTCTCATGGCTCGCCTGGCCAGTCAGACATACCATCAATTGCTGCGGTTGTCAGCTCGAGGCACTGGCCACTCATTTCTCGCTATAGGGCATCTGTTCGAACTCAGTCTCCAAAGGTCGAAATGATTGATTCACTGTTCAAACGTGTTTCTGATACAGAAGATGATGGCATTATGAGAGAGCTGCTTTTGGACTTCTATACAAGTTCAGGAAAGCGTAAACCAGATCAGATAATCATATTCAGGGATGGTGTTAGCGAGTCGCAGTTCAATCAAGTCTTAAACATTGAGCTGGACCAGATCATTGAGGCCTGCAAGTTCCTTGATGAAAGTTGGTGCCCAAAATTTGTGGTGATTATCGCGCAGAAGAACCACCACACAAAATTCTTCCAGCCTCAGTCACCTGATAATGTTCCACCTGGTACCATTATTGACAACAGAATTTGTCATCCGAAGAACAATGATTTTTATCTCTGTGCTCAGGCTGGAATGATTGGAACCACGAGGCCTACCCATTATCATGTTCTGTTGGATGATGTTGGGTTTTCAGCTGATGATCTGCAGGAACTAGTGCATTCTCTGTCCTATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003682 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0008150 GO:0008152 GO:0008213 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009814 GO:0009816 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015030 GO:0016032 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016604 GO:0016787 GO:0016788 GO:0018022 GO:0018193 GO:0018205 GO:0019048 GO:0019219 GO:0019222 GO:0019538 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0034641 GO:0034968 GO:0035194 GO:0035197 GO:0035198 GO:0035821 GO:0036211 GO:0040029 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044728 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0060255 GO:0061647 GO:0061980 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0140098 GO:1901360 GO:1901363 GO:1901564 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

877

Amino Acids

97.61

Weight (kDa)

8.91

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 86 - 249 1e-30 N-terminal domain of argonaute
ArgoL1 PF08699 261 - 310 1.3e-18 Argonaute linker 1 domain
PAZ PF02170 315 - 444 3.6e-29 PAZ domain
ArgoL2 PF16488 454 - 499 1.1e-12 Argonaute linker 2 domain
Piwi PF02171 597 - 905 6.6e-105 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 615
AatII GACGTC 1 cut(s) 613
Acc65I GGTACC 1 cut(s) 2459
AccB1I GGYRCC 2 cut(s) 2373, 2459
AccI GTMKAC 1 cut(s) 1295
AccII CGCG 2 cut(s) 1473, 2399
AccIII TCCGGA 1 cut(s) 1807
AciI CCGC 9 cut(s) 116, 691, 1471, 1534, 1605, 1724, 1784, 1846, 2084
AclWI GGATC 1 cut(s) 971
AcoI YGGCCR 4 cut(s) 1468, 1857, 2056, 2105
AcuI CTGAAG 2 cut(s) 853, 1037
AcyI GRCGYC 1 cut(s) 610
AdeI CACNNNGTG 2 cut(s) 1613, 1881
AfaI GTAC 3 cut(s) 622, 1163, 2461
AfiI CCNNNNNNNGG 7 cut(s) 46, 371, 372, 1529, 1530, 1729, 2032
AflIII ACRYGT 1 cut(s) 2182
AhdI GACNNNNNGTC 1 cut(s) 64
AhlI ACTAGT 1 cut(s) 2605
AjiI CACGTC 1 cut(s) 1718
AjnI CCWGG 6 cut(s) 613, 879, 931, 1774, 2053, 2455
AjuI GAANNNNNNNTTGG 2 cut(s) 1952, 1984
AloI GAACNNNNNNTCC 6 cut(s) 309, 341, 822, 854, 2131, 2163
Alw21I GWGCWC 1 cut(s) 2518
Alw26I GTCTC 7 cut(s) 51, 407, 779, 1075, 1708, 2046, 2151
AlwI GGATC 1 cut(s) 971
Ama87I CYCGRG 1 cut(s) 2095
Aor13HI TCCGGA 1 cut(s) 1807
AoxI GGCC 8 cut(s) 238, 886, 1468, 1857, 2056, 2105, 2346, 2543
ApeKI GCWGC 3 cut(s) 653, 2081, 2221
ArsI GACNNNNNNTTYG 6 cut(s) 279, 311, 1667, 1699, 1909, 1941
AseI ATTAAT 3 cut(s) 558, 1224, 1938
Asp718I GGTACC 1 cut(s) 2459
AspA2I CCTAGG 1 cut(s) 2026
AspLEI GCGC 1 cut(s) 2401
AspS9I GGNCC 3 cut(s) 935, 1835, 2332
AsuHPI GGTGA 7 cut(s) 470, 581, 1145, 1421, 1977, 2401, 2430
AsuII TTCGAA 1 cut(s) 2137
AvaI CYCGRG 1 cut(s) 2095
AvaII GGWCC 3 cut(s) 935, 1835, 2332
AvrII CCTAGG 1 cut(s) 2026
BaeGI GKGCMC 2 cut(s) 1723, 2378
BalI TGGCCA 2 cut(s) 2058, 2107
BanI GGYRCC 2 cut(s) 2373, 2459
BauI CACGAG 1 cut(s) 2539
BbsI GAAGAC 1 cut(s) 360
Bbv12I GWGCWC 1 cut(s) 2518
BbvI GCAGC 3 cut(s) 640, 2068, 2208
BciT130I CCWGG 6 cut(s) 615, 881, 933, 1776, 2055, 2457
BciVI GTATCC 1 cut(s) 520
BclI TGATCA 1 cut(s) 921
BcoDI GTCTC 7 cut(s) 51, 407, 779, 1075, 1708, 2046, 2151
BcuI ACTAGT 1 cut(s) 2605
BfaI CTAG 6 cut(s) 231, 779, 842, 1052, 2027, 2606
BfmI CTRYAG 2 cut(s) 2122, 2597
BfuI GTATCC 1 cut(s) 520
BglII AGATCT 1 cut(s) 1322
BisI GCNGC 5 cut(s) 117, 654, 1471, 2082, 2222
BlnI CCTAGG 1 cut(s) 2026
BlsI GCNGC 5 cut(s) 118, 655, 1472, 2083, 2223
Bme1390I CCNGG 6 cut(s) 615, 881, 933, 1776, 2055, 2457
Bme18I GGWCC 3 cut(s) 935, 1835, 2332
BmeRI GACNNNNNGTC 1 cut(s) 64
BmeT110I CYCGRG 1 cut(s) 2095
BmgBI CACGTC 1 cut(s) 1718
BmgT120I GGNCC 3 cut(s) 935, 1835, 2332
BmiI GGNNCC 4 cut(s) 2013, 2375, 2461, 2536
BmrFI CCNGG 6 cut(s) 615, 881, 933, 1776, 2055, 2457
BmsI GCATC 3 cut(s) 767, 1380, 2138
BoxI GACNNNNGTC 2 cut(s) 35, 360
BpiI GAAGAC 1 cut(s) 360
BpmI CTGGAG 2 cut(s) 111, 542
Bpu10I CCTNAGC 1 cut(s) 2517
Bpu14I TTCGAA 1 cut(s) 2137
BpuEI CTTGAG 1 cut(s) 1283
BsaAI YACGTR 1 cut(s) 1424
BsaBI GATNNNNATC 2 cut(s) 1370, 2505
BsaHI GRCGYC 1 cut(s) 610
BsaI GGTCTC 2 cut(s) 51, 1075
BsaJI CCNNGG 7 cut(s) 61, 241, 879, 880, 1838, 2007, 2026
BsaWI WCCGGW 1 cut(s) 1807
BsaXI ACNNNNNCTCC 4 cut(s) 822, 852, 2131, 2161
Bsc4I CCNNNNNNNGG 7 cut(s) 46, 371, 372, 1529, 1530, 1729, 2032
Bse1I ACTGG 7 cut(s) 94, 938, 998, 1072, 1335, 2059, 2108
Bse3DI GCAATG 3 cut(s) 128, 1283, 1510
Bse8I GATNNNNATC 2 cut(s) 1370, 2505
BseAI TCCGGA 1 cut(s) 1807
BseBI CCWGG 6 cut(s) 615, 881, 933, 1776, 2055, 2457
BseDI CCNNGG 7 cut(s) 61, 241, 879, 880, 1838, 2007, 2026
BseJI GATNNNNATC 2 cut(s) 1370, 2505
BseLI CCNNNNNNNGG 7 cut(s) 46, 371, 372, 1529, 1530, 1729, 2032
BseMI GCAATG 3 cut(s) 128, 1283, 1510
BseMII CTCAG 4 cut(s) 716, 2156, 2447, 2531
BseNI ACTGG 7 cut(s) 94, 938, 998, 1072, 1335, 2059, 2108
BseRI GAGGAG 1 cut(s) 846
BseSI GKGCMC 2 cut(s) 1723, 2378
BseXI GCAGC 3 cut(s) 640, 2068, 2208
BsgI GTGCAG 2 cut(s) 198, 868
Bsh1236I CGCG 2 cut(s) 1473, 2399
Bsh1285I CGRYCG 1 cut(s) 361
BshFI GGCC 8 cut(s) 240, 888, 1470, 1859, 2058, 2107, 2348, 2545
BshNI GGYRCC 2 cut(s) 2373, 2459
BsiEI CGRYCG 1 cut(s) 361
BsiHKAI GWGCWC 1 cut(s) 2518
BsiHKCI CYCGRG 1 cut(s) 2095
BsiSI CCGG 1 cut(s) 1808
BslFI GGGAC 2 cut(s) 43, 348
BslI CCNNNNNNNGG 7 cut(s) 46, 371, 372, 1529, 1530, 1729, 2032
BsmAI GTCTC 7 cut(s) 51, 407, 779, 1075, 1708, 2046, 2151
BsmBI CGTCTC 1 cut(s) 1708
BsmFI GGGAC 2 cut(s) 43, 348
BsmI GAATGC 1 cut(s) 2611
BsnI GGCC 8 cut(s) 240, 888, 1470, 1859, 2058, 2107, 2348, 2545
Bso31I GGTCTC 2 cut(s) 51, 1075
BsoBI CYCGRG 1 cut(s) 2095
Bsp119I TTCGAA 1 cut(s) 2137
Bsp1286I GDGCHC 3 cut(s) 1723, 2378, 2518
Bsp13I TCCGGA 1 cut(s) 1807
Bsp19I CCATGG 1 cut(s) 1838
BspACI CCGC 9 cut(s) 116, 691, 1471, 1534, 1605, 1724, 1784, 1846, 2084
BspANI GGCC 8 cut(s) 240, 888, 1470, 1859, 2058, 2107, 2348, 2545
BspCNI CTCAG 4 cut(s) 717, 2155, 2446, 2530
BspEI TCCGGA 1 cut(s) 1807
BspFNI CGCG 2 cut(s) 1473, 2399
BspLI GGNNCC 4 cut(s) 2013, 2375, 2461, 2536
BspMAI CTGCAG 1 cut(s) 2601
BspPI GGATC 1 cut(s) 971
BspT104I TTCGAA 1 cut(s) 2137
BspT107I GGYRCC 2 cut(s) 2373, 2459
BspTNI GGTCTC 2 cut(s) 51, 1075
BsrDI GCAATG 3 cut(s) 128, 1283, 1510
BsrI ACTGG 7 cut(s) 94, 938, 998, 1072, 1335, 2059, 2108
BssECI CCNNGG 7 cut(s) 61, 241, 879, 880, 1838, 2007, 2026
BssNAI GTATAC 1 cut(s) 1296
BssNI GRCGYC 1 cut(s) 610
BssSI CACGAG 1 cut(s) 2539
BssT1I CCWWGG 4 cut(s) 241, 1838, 2007, 2026
Bst1107I GTATAC 1 cut(s) 1296
Bst2BI CACGAG 1 cut(s) 2539
Bst2UI CCWGG 6 cut(s) 615, 881, 933, 1776, 2055, 2457
Bst4CI ACNGT 9 cut(s) 335, 359, 478, 1159, 1194, 1293, 1710, 1881, 2175
Bst6I CTCTTC 3 cut(s) 458, 593, 1692
BstACI GRCGYC 1 cut(s) 610
BstAPI GCANNNNNTGC 1 cut(s) 185
BstBAI YACGTR 1 cut(s) 1424
BstBI TTCGAA 1 cut(s) 2137
BstC8I GCNNGC 5 cut(s) 177, 1482, 1609, 2051, 2350
BstDEI CTNAG 8 cut(s) 725, 762, 1005, 1488, 1572, 2142, 2433, 2517
BstDSI CCRYGG 2 cut(s) 61, 1838
BstFNI CGCG 2 cut(s) 1473, 2399
BstHHI GCGC 1 cut(s) 2401
BstMAI GTCTC 7 cut(s) 51, 407, 779, 1075, 1708, 2046, 2151
BstMCI CGRYCG 1 cut(s) 361
BstMWI GCNNNNNNNGC 3 cut(s) 125, 185, 2055
BstNI CCWGG 6 cut(s) 615, 881, 933, 1776, 2055, 2457
BstNSI RCATGY 2 cut(s) 758, 1431
BstPAI GACNNNNGTC 2 cut(s) 35, 360
BstSCI CCNGG 6 cut(s) 613, 879, 931, 1774, 2053, 2455
BstSFI CTRYAG 2 cut(s) 2122, 2597
BstSLI GKGCMC 2 cut(s) 1723, 2378
BstUI CGCG 2 cut(s) 1473, 2399
BstV1I GCAGC 3 cut(s) 640, 2068, 2208
BstV2I GAAGAC 1 cut(s) 360
BstX2I RGATCY 2 cut(s) 976, 1322
BstXI CCANNNNNNTGG 1 cut(s) 1999
BstYI RGATCY 2 cut(s) 976, 1322
BstZ17I GTATAC 1 cut(s) 1296
BsuI GTATCC 1 cut(s) 520
BsuRI GGCC 8 cut(s) 240, 888, 1470, 1859, 2058, 2107, 2348, 2545
BtgI CCRYGG 2 cut(s) 61, 1838
BtrI CACGTC 1 cut(s) 1718
BtsIMutI CAGTG 6 cut(s) 340, 474, 1328, 1886, 2101, 2171
Cac8I GCNNGC 5 cut(s) 177, 1482, 1609, 2051, 2350
CfoI GCGC 1 cut(s) 2401
Cfr13I GGNCC 3 cut(s) 935, 1835, 2332
CseI GACGC 2 cut(s) 773, 1232
CsiI ACCWGGT 1 cut(s) 2455
Csp6I GTAC 3 cut(s) 621, 1162, 2460
CspCI CAANNNNNGTGG 2 cut(s) 2367, 2402
CviAII CATG 9 cut(s) 410, 667, 755, 919, 1086, 1428, 1839, 2045, 2558
CviQI GTAC 3 cut(s) 621, 1162, 2460
DdeI CTNAG 8 cut(s) 725, 762, 1005, 1488, 1572, 2142, 2433, 2517
DraIII CACNNNGTG 2 cut(s) 1613, 1881
DrdI GACNNNNNNGTC 1 cut(s) 615
DriI GACNNNNNGTC 1 cut(s) 64
DseDI GACNNNNNNGTC 1 cut(s) 615
EaeI YGGCCR 4 cut(s) 1468, 1857, 2056, 2105
Eam1104I CTCTTC 3 cut(s) 458, 593, 1692
Eam1105I GACNNNNNGTC 1 cut(s) 64
EarI CTCTTC 3 cut(s) 458, 593, 1692
Eco130I CCWWGG 4 cut(s) 241, 1838, 2007, 2026
Eco147I AGGCCT 3 cut(s) 240, 2348, 2545
Eco31I GGTCTC 2 cut(s) 51, 1075
Eco47I GGWCC 3 cut(s) 935, 1835, 2332
Eco57I CTGAAG 2 cut(s) 853, 1037
Eco88I CYCGRG 1 cut(s) 2095
EcoRI GAATTC 1 cut(s) 1960
EcoRII CCWGG 6 cut(s) 613, 879, 931, 1774, 2053, 2455
EcoT14I CCWWGG 4 cut(s) 241, 1838, 2007, 2026
EcoT22I ATGCAT 1 cut(s) 760
ErhI CCWWGG 4 cut(s) 241, 1838, 2007, 2026
Esp3I CGTCTC 1 cut(s) 1708
FaeI CATG 9 cut(s) 413, 670, 758, 922, 1089, 1431, 1842, 2048, 2561
FaqI GGGAC 2 cut(s) 43, 348
FatI CATG 9 cut(s) 409, 666, 754, 918, 1085, 1427, 1838, 2044, 2557
FauI CCCGC 2 cut(s) 684, 1731
FauNDI CATATG 1 cut(s) 451
FbaI TGATCA 1 cut(s) 921
FblI GTMKAC 1 cut(s) 1295
Fnu4HI GCNGC 5 cut(s) 117, 654, 1471, 2082, 2222
Fsp4HI GCNGC 5 cut(s) 117, 654, 1471, 2082, 2222
FspBI CTAG 6 cut(s) 231, 779, 842, 1052, 2027, 2606
GlaI GCGC 1 cut(s) 2400
GluI GCNGC 5 cut(s) 117, 654, 1471, 2082, 2222
GsuI CTGGAG 2 cut(s) 111, 542
HaeIII GGCC 8 cut(s) 240, 888, 1470, 1859, 2058, 2107, 2348, 2545
HapII CCGG 1 cut(s) 1808
HgaI GACGC 2 cut(s) 773, 1232
HhaI GCGC 1 cut(s) 2401
Hin1I GRCGYC 1 cut(s) 610
Hin1II CATG 9 cut(s) 413, 670, 758, 922, 1089, 1431, 1842, 2048, 2561
Hin6I GCGC 1 cut(s) 2399
HinP1I GCGC 1 cut(s) 2399
HincII GTYRAC 2 cut(s) 273, 1597
HindII GTYRAC 2 cut(s) 273, 1597
HindIII AAGCTT 2 cut(s) 865, 1946
HpaI GTTAAC 2 cut(s) 273, 1597
HpaII CCGG 1 cut(s) 1808
HphI GGTGA 7 cut(s) 470, 581, 1145, 1421, 1977, 2401, 2430
Hpy188III TCNNGA 6 cut(s) 521, 698, 1052, 1713, 1808, 2247
Hpy99I CGWCG 1 cut(s) 612
HpyAV CCTTC 7 cut(s) 370, 384, 566, 664, 1325, 1654, 1998
HpyCH4III ACNGT 9 cut(s) 335, 359, 478, 1159, 1194, 1293, 1710, 1881, 2175
HpyCH4IV ACGT 6 cut(s) 294, 610, 1423, 1689, 1717, 2182
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 185, 2055
HpyF3I CTNAG 8 cut(s) 725, 762, 1005, 1488, 1572, 2142, 2433, 2517
HpySE526I ACGT 6 cut(s) 294, 610, 1423, 1689, 1717, 2182
Hsp92I GRCGYC 1 cut(s) 610
Hsp92II CATG 9 cut(s) 413, 670, 758, 922, 1089, 1431, 1842, 2048, 2561
HspAI GCGC 1 cut(s) 2399
Kpn2I TCCGGA 1 cut(s) 1807
KpnI GGTACC 1 cut(s) 2463
Ksp22I TGATCA 1 cut(s) 921
KspAI GTTAAC 2 cut(s) 273, 1597
LmnI GCTCC 3 cut(s) 1489, 1894, 2017
Lsp1109I GCAGC 3 cut(s) 640, 2068, 2208
LweI GCATC 3 cut(s) 767, 1380, 2138
MabI ACCWGGT 1 cut(s) 2455
MaeI CTAG 6 cut(s) 231, 779, 842, 1052, 2027, 2606
MaeII ACGT 6 cut(s) 294, 610, 1423, 1689, 1717, 2182
MaeIII GTNAC 6 cut(s) 295, 305, 343, 1153, 1873, 2436
MfeI CAATTG 5 cut(s) 225, 675, 1097, 1440, 2076
MflI RGATCY 2 cut(s) 976, 1322
MhlI GDGCHC 3 cut(s) 1723, 2378, 2518
MlsI TGGCCA 2 cut(s) 2058, 2107
MluNI TGGCCA 2 cut(s) 2058, 2107
MlyI GAGTC 4 cut(s) 414, 586, 710, 2306
MmeI TCCRAC 3 cut(s) 808, 1271, 2547
Mox20I TGGCCA 2 cut(s) 2058, 2107
Mph1103I ATGCAT 1 cut(s) 760
MroI TCCGGA 1 cut(s) 1807
MscI TGGCCA 2 cut(s) 2058, 2107
MslI CAYNNNNRTG 3 cut(s) 333, 1997, 2556
Msp20I TGGCCA 2 cut(s) 2058, 2107
MspA1I CMGCKG 1 cut(s) 2588
MspI CCGG 1 cut(s) 1808
MspR9I CCNGG 6 cut(s) 615, 881, 933, 1776, 2055, 2457
MunI CAATTG 5 cut(s) 225, 675, 1097, 1440, 2076
Mva1269I GAATGC 1 cut(s) 2611
MvaI CCWGG 6 cut(s) 615, 881, 933, 1776, 2055, 2457
MvnI CGCG 2 cut(s) 1473, 2399
MwoI GCNNNNNNNGC 3 cut(s) 125, 185, 2055
NcoI CCATGG 1 cut(s) 1838
NdeI CATATG 1 cut(s) 451
NlaIII CATG 9 cut(s) 413, 670, 758, 922, 1089, 1431, 1842, 2048, 2561
NlaIV GGNNCC 4 cut(s) 2013, 2375, 2461, 2536
NmeAIII GCCGAG 1 cut(s) 1885
NmuCI GTSAC 4 cut(s) 295, 1153, 1873, 2436
NsiI ATGCAT 1 cut(s) 760
NspI RCATGY 2 cut(s) 758, 1431
NspV TTCGAA 1 cut(s) 2137
PaeR7I CTCGAG 1 cut(s) 2095
PasI CCCWGGG 1 cut(s) 880
PceI AGGCCT 3 cut(s) 240, 2348, 2545
PcsI WCGNNNNNNNCGW 1 cut(s) 616
PctI GAATGC 1 cut(s) 2611
PfeI GAWTC 6 cut(s) 5, 262, 855, 1702, 1852, 2168
PfoI TCCNGGA 1 cut(s) 1774
PkrI GCNGC 5 cut(s) 118, 655, 1472, 2083, 2223
PleI GAGTC 4 cut(s) 413, 585, 709, 2305
PpsI GAGTC 4 cut(s) 413, 585, 709, 2305
Ppu21I YACGTR 1 cut(s) 1424
PshAI GACNNNNGTC 2 cut(s) 35, 360
PshBI ATTAAT 3 cut(s) 558, 1224, 1938
Psp6I CCWGG 6 cut(s) 613, 879, 931, 1774, 2053, 2455
PspGI CCWGG 6 cut(s) 613, 879, 931, 1774, 2053, 2455
PspN4I GGNNCC 4 cut(s) 2013, 2375, 2461, 2536
PspPI GGNCC 3 cut(s) 935, 1835, 2332
PspXI VCTCGAGB 1 cut(s) 2095
PsrI GAACNNNNNNTAC 2 cut(s) 1404, 1436
PstI CTGCAG 1 cut(s) 2601
PsuI RGATCY 2 cut(s) 976, 1322
PvuII CAGCTG 1 cut(s) 2588
RsaI GTAC 3 cut(s) 622, 1163, 2461
RsaNI GTAC 3 cut(s) 621, 1162, 2460
RseI CAYNNNNRTG 3 cut(s) 333, 1997, 2556
SatI GCNGC 5 cut(s) 117, 654, 1471, 2082, 2222
Sau96I GGNCC 3 cut(s) 935, 1835, 2332
SchI GAGTC 4 cut(s) 414, 586, 710, 2306
ScrFI CCNGG 6 cut(s) 615, 881, 933, 1776, 2055, 2457
SduI GDGCHC 3 cut(s) 1723, 2378, 2518
SexAI ACCWGGT 1 cut(s) 2455
SfaNI GCATC 3 cut(s) 767, 1380, 2138
SfcI CTRYAG 2 cut(s) 2122, 2597
Sfr274I CTCGAG 1 cut(s) 2095
SfuI TTCGAA 1 cut(s) 2137
SinI GGWCC 3 cut(s) 935, 1835, 2332
SlaI CTCGAG 1 cut(s) 2095
SmiMI CAYNNNNRTG 3 cut(s) 333, 1997, 2556
SmlI CTYRAG 2 cut(s) 1262, 2095
SmoI CTYRAG 2 cut(s) 1262, 2095
SpeI ACTAGT 1 cut(s) 2605
SseBI AGGCCT 3 cut(s) 240, 2348, 2545
SsiI CCGC 9 cut(s) 116, 691, 1471, 1534, 1605, 1724, 1784, 1846, 2084
SspI AATATT 1 cut(s) 1749
SspMI CTAG 6 cut(s) 231, 779, 842, 1052, 2027, 2606
StuI AGGCCT 3 cut(s) 240, 2348, 2545
StyD4I CCNGG 6 cut(s) 613, 879, 931, 1774, 2053, 2455
StyI CCWWGG 4 cut(s) 241, 1838, 2007, 2026
TaaI ACNGT 9 cut(s) 335, 359, 478, 1159, 1194, 1293, 1710, 1881, 2175
TaiI ACGT 6 cut(s) 297, 613, 1426, 1692, 1720, 2185
TaqI TCGA 5 cut(s) 403, 1712, 2096, 2137, 2157
TauI GCSGC 2 cut(s) 119, 1473
TfiI GAWTC 6 cut(s) 5, 262, 855, 1702, 1852, 2168
TscAI CASTG 6 cut(s) 340, 481, 1335, 1886, 2108, 2178
TseFI GTSAC 4 cut(s) 295, 1153, 1873, 2436
TseI GCWGC 3 cut(s) 653, 2081, 2221
Tsp45I GTSAC 4 cut(s) 295, 1153, 1873, 2436
TspDTI ATGAA 7 cut(s) 366, 600, 683, 1272, 1673, 1780, 2379
TspGWI ACGGA 2 cut(s) 159, 1622
TspRI CASTG 6 cut(s) 340, 481, 1335, 1886, 2108, 2178
VpaK11BI GGWCC 3 cut(s) 935, 1835, 2332
VspI ATTAAT 3 cut(s) 558, 1224, 1938
XbaI TCTAGA 1 cut(s) 1051
XceI RCATGY 2 cut(s) 758, 1431
XhoI CTCGAG 1 cut(s) 2095
XmaJI CCTAGG 1 cut(s) 2026
XmiI GTMKAC 1 cut(s) 1295
XspI CTAG 6 cut(s) 231, 779, 842, 1052, 2027, 2606
ZraI GACGTC 1 cut(s) 611
Zsp2I ATGCAT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.