MD11G1182100.v1.1

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0006994
Physical Location & Seq
Reverse (-)
22420492 .. 22428731
8240 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1182100.v1.1.491

Sequence Viewer

Length: 2982 bp
ATGGTTAGTAGTTGTTGGTGGAAAAGGAGTGAGAAAGTAGGAGAGAGAGAGAGTTCCTCCCTCGGTCGTCAGTCTCATCAGTCGTCTCTCTCAGCCGTTTTAGGGTTTCTCAGGGTTGAAGAGTACTCAGTGCTGGGTGTTTCGTGTGCTGTTAGTATGGATATGGATTCATTTGAGCCAGATGGAAATGGAGTAAATGGAGCAAATGGTGTGAATGGAGAAAATGGTGTGAATGGAACAAATGGGATGAATGGAGCAAATGGGGCAGAAGATGCGTTGCCACCCCCACCCCCAGTTATTCCACCAGATGTTGTTCCTTTGCGTGCGGAAGCAGATAAAATTCCCGAACCTGTTAAGAAGAAACCTGCTCGGGCCCCTATTGCTAGGCGTGGCCAAGGAACCAAGGGAACAAAAATACCTTTGGTCACAAATCACTTCAAAGTGAACGTTACTAATATTGAGGGTTACTTCTTCCATTATAGTGTTTCTGTTGCTTATGAAGATGGCCGTCCCCTTGATGGAAAGGGTGCTGGAAGAAGAATAATTGAAAGGGTGCATGAGACCTATCAGTCTGAGTTAGGTGGAAAGGACTTTGCCTATGATGGAGAGAAGAGCCTGTTTACTGTGGGTTCCCTACCACGCAACAAACTTGAATTTGCCGTAGTTTTGGAGGATGTGCCATCAAGCAGGAACAATGGAACCAGTGAACCTGATGGTCCTGGGAGTCCAAATGAGAGTGACCGAAAGAGATTACGGCGTCCAAATCGCTCCAAAACATTCAATGTGGAGATTAGCTATGCTGCAAAAATTCCCATGAAAGCAATTGGAGATGCTCTGCGTGGGCAAGAATCGGAGAATTCTCAAGAAGCTTTGAGAGTTCTGGATATCATACTAAGACAACATGCATCAAAGCAAGGATGCCTCCTAGTTCGCCAGTCATTCTTCCATAACGATCCAAAAAATTTTGCGGATGTTGGAGGCGGTGTTCTTGGATGTAGAGGATTCCATTCAAGTTTTAGGACCACCCAGGGTGGCTTGTCTTTGAATATTGATGTATCGACTACCATGATTATACAGCCAGGGCCAGTGGTGGACTTTCTAATTGCCAACCAAAATGTTAGAGATCCGTTCCAACTTGACTGGACGAAGGCTAAAAGAACACTCAAGAATTTGAGGGTCAAAACAAGTCCATCAAATCTTGAGTACAAGATATCTGGATTGAGCGAGAAACCATGCCGAGAACAAACTTTTACATTGAGAAACAAACAAGCGAAGGAAGGTGAAGATGCAGAAATAGAAGTCACTGTTTATGATTATTTTGTTAATCATCGTAATATACAACTGCGTTACTCTGCTGATCTACCGTGCCTTAATGTTGGGAAGCCAAAACGTCCTACCTATATCCCCCTTGAGCTTTGTTCTTTGGTGTCCTTACAACGTTACACCAAAGCTCTTTCCACCCTTCAAAGAGCTTCGCTGGTGGAAAAATCAAGGCAAAAGCCACAAGAGAGGATGAGTGTTTTGTCTAATGCTCTGAAAATCAACAATTATGATGCTGAACCAATGCTACGCTCATGCGGTGTTTCAATTGGTGGTAACTTTACTCAAGTGGAAGGCCGTGTTCTTCCAGCCCCAAAGCTTAAAGTGGGTAACGGAGATGATTTCTTCCCTCGAAATGGGCGCTGGAATTTCAACAACAAGACACTTGTGAAGCCAACTAAGATAGAAAAGTGGGCTGTTGTCAACTTCTCCGCACGCTGTGATCTAAAAGCCCTTGTCCGCGATCTAATCAAATGTGGTGATATGAAAGGAATTAAAATCGAGGCTCCATTTGATGTGTTTGAAGAGAATCCCCAGTCTAGGCGTGCCCCACCTTTGGTTAGAGTTGAGAGGATGTTTGAGGATATACAGTCCAAACTTCCTGGACAACCACAGTTTCTTCTCTGTTTGCTGCCGGAGAGGAAAAATTCTGCTTTATATGGTCCATGGAAGCGGAAGAATCTTGCTGAGTATGGTATTGTGACACAGTGCATTGCTCCCACGAGGGTCAATGATCAATACCTTACAAATGTCCTGTTAAAAATCAATGCAAAGCTTGGTGGTTTAAATTCATTGTTGGCAGTTGAATATTCTCCTTCTATCCCTGTGGTTTCCAAGGCTCCCACTATCATCCTTGGGATGGATGTGTCCCATGGTTCACCTGGGCAGTCTGATGTACCTTCAATTGCCGCGGTGGTCAGCTCCAGGCAGTGGCCCCTGATATCTCGCTACAGGGCATCTGTTCGAACACAATCCCCCAAGGTGGAGATGATAGACTCGCTGTACAAGCGAGTATCTGACACAGAGGATGATGGCATTATGAGAGAGCTGCTTCTGGACTTCTATACAAGTTCAGGCAAACAGAAACCGGACCAGATTATCATATTCAGGGATGGGGTTAGCGAGTCTCAATTCAACCAAGTTTTGAACATTGAACTGGATCAAATCATAGAGGCATGCAAGTTCCTGGATGAGGCTTGGTCCCCTAAATTTGTGGTGATTATAGCTCAGAAAAACCACCACACAAAGTTCTTTCAGCCTCAGTCCCCCGATAACGTTCCTCCTGGAACAATTATAGATAACAGAATTTGTCACCCAAGGAACAATGACTTCTATCTCTGTGCCCAAGCGGGAATGATTGGGACCACGAGGCCTACTCACTATCATGTTCTGTTAGACGACGTTGGTTTTTCAGCCGATGATCTGCAGGAACTAGTGCATTCTCTTTCATACGTGTACCAGAGAAGTACCACCGCCATTTCCGTTGTTGCCCCCATTTGCTATGCACATTTAGCTGCCACTCAGATGTCACAGTTCATGAAATTTGAAGACGCGTCTGAGACATCTTCAAGCCATGGCGGGCTGACCTCCGCTGGAGCTGTTCCTGTCCCTCAGCTGCCCAGGTTGAAGGAGAATGTGTCCAATTCCATGTTTTTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003682 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0008150 GO:0008152 GO:0008213 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009814 GO:0009816 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015030 GO:0016032 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016604 GO:0016787 GO:0016788 GO:0018022 GO:0018193 GO:0018205 GO:0019048 GO:0019219 GO:0019222 GO:0019538 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0034641 GO:0034968 GO:0035194 GO:0035197 GO:0035198 GO:0035821 GO:0036211 GO:0040029 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044728 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0060255 GO:0061647 GO:0061980 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0140098 GO:1901360 GO:1901363 GO:1901564 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

994

Amino Acids

110.08

Weight (kDa)

8.86

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 137 - 298 2.9e-30 N-terminal domain of argonaute
ArgoL1 PF08699 310 - 359 5.8e-19 Argonaute linker 1 domain
PAZ PF02170 364 - 493 2.4e-31 PAZ domain
ArgoL2 PF16488 503 - 548 2.2e-12 Argonaute linker 2 domain
ArgoMid PF16487 557 - 609 9.9e-07 Mid domain of argonaute
Piwi PF02171 647 - 954 2.9e-105 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 568
Acc36I ACCTGC 1 cut(s) 373
AccB7I CCANNNNNTGG 1 cut(s) 2250
AccII CGCG 3 cut(s) 1782, 2231, 2873
AclI AACGTT 3 cut(s) 447, 1438, 2595
AclWI GGATC 3 cut(s) 947, 1118, 2487
AcoI YGGCCR 2 cut(s) 391, 505
AcyI GRCGYC 1 cut(s) 757
AdeI CACNNNGTG 1 cut(s) 1760
AfaI GTAC 6 cut(s) 125, 1205, 2217, 2324, 2777, 2788
AfiI CCNNNNNNNGG 9 cut(s) 102, 518, 1676, 1860, 1876, 2179, 2250, 2302, 2512
AflIII ACRYGT 2 cut(s) 2772, 2871
AhlI ACTAGT 1 cut(s) 2752
AjnI CCWGG 9 cut(s) 718, 1026, 1078, 1921, 2200, 2243, 2505, 2602, 2939
AloI GAACNNNNNNTCC 2 cut(s) 969, 1001
Alw26I GTCTC 5 cut(s) 78, 90, 554, 2451, 2873
AlwI GGATC 3 cut(s) 947, 1118, 2487
Ama87I CYCGRG 1 cut(s) 369
AoxI GGCC 7 cut(s) 372, 391, 505, 1082, 1615, 2252, 2690
ApaI GGGCCC 1 cut(s) 376
ApeKI GCWGC 5 cut(s) 800, 1951, 2368, 2834, 2935
AspLEI GCGC 1 cut(s) 1683
AsuHPI GGTGA 5 cut(s) 1292, 1811, 2190, 2548, 2624
AsuII TTCGAA 1 cut(s) 2284
AvaI CYCGRG 1 cut(s) 369
AvaII GGWCC 6 cut(s) 716, 1020, 1982, 2410, 2520, 2682
BaeGI GKGCMC 3 cut(s) 376, 1870, 2665
BalI TGGCCA 1 cut(s) 393
BanII GRGCYC 1 cut(s) 376
BauI CACGAG 2 cut(s) 2041, 2686
BbsI GAAGAC 1 cut(s) 2874
BbvCI CCTCAGC 1 cut(s) 2931
BbvI GCAGC 5 cut(s) 787, 1938, 2355, 2821, 2922
BceAI ACGGC 5 cut(s) 80, 492, 644, 770, 1602
BciT130I CCWGG 9 cut(s) 720, 1028, 1080, 1923, 2202, 2245, 2507, 2604, 2941
BclI TGATCA 1 cut(s) 2053
BcoDI GTCTC 5 cut(s) 78, 90, 554, 2451, 2873
BcuI ACTAGT 1 cut(s) 2752
BfaI CTAG 4 cut(s) 384, 926, 1860, 2753
BfmI CTRYAG 2 cut(s) 2269, 2744
BfoI RGCGCY 1 cut(s) 1684
BfuAI ACCTGC 1 cut(s) 373
BisI GCNGC 6 cut(s) 801, 1952, 2229, 2369, 2835, 2936
BlsI GCNGC 6 cut(s) 802, 1953, 2230, 2370, 2836, 2937
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 9 cut(s) 720, 1028, 1080, 1923, 2202, 2245, 2507, 2604, 2941
Bme18I GGWCC 6 cut(s) 716, 1020, 1982, 2410, 2520, 2682
BmeT110I CYCGRG 1 cut(s) 369
BmrFI CCNGG 9 cut(s) 720, 1028, 1080, 1923, 2202, 2245, 2507, 2604, 2941
BmrI ACTGGG 2 cut(s) 287, 1849
BmsI GCATC 7 cut(s) 262, 820, 908, 914, 1276, 1543, 2285
BmuI ACTGGG 2 cut(s) 287, 1849
BpiI GAAGAC 1 cut(s) 2874
BplI GAGNNNNNCTC 4 cut(s) 41, 73, 2680, 2712
BpmI CTGGAG 2 cut(s) 2227, 2934
Bpu10I CCTNAGC 1 cut(s) 2931
Bpu14I TTCGAA 1 cut(s) 2284
BpuEI CTTGAG 5 cut(s) 846, 1148, 1220, 1430, 1590
BsaAI YACGTR 1 cut(s) 2773
BsaHI GRCGYC 1 cut(s) 757
BsaI GGTCTC 1 cut(s) 554
BsaWI WCCGGW 1 cut(s) 2407
BsaXI ACNNNNNCTCC 4 cut(s) 969, 999, 2942, 2972
Bsc4I CCNNNNNNNGG 9 cut(s) 102, 518, 1676, 1860, 1876, 2179, 2250, 2302, 2512
Bse1I ACTGG 7 cut(s) 293, 702, 934, 1085, 1145, 1855, 2481
Bse3DI GCAATG 1 cut(s) 2031
BseBI CCWGG 9 cut(s) 720, 1028, 1080, 1923, 2202, 2245, 2507, 2604, 2941
BseLI CCNNNNNNNGG 9 cut(s) 102, 518, 1676, 1860, 1876, 2179, 2250, 2302, 2512
BseMI GCAATG 1 cut(s) 2031
BseNI ACTGG 7 cut(s) 293, 702, 934, 1085, 1145, 1855, 2481
BseSI GKGCMC 3 cut(s) 376, 1870, 2665
BseXI GCAGC 5 cut(s) 787, 1938, 2355, 2821, 2922
BseYI CCCAGC 1 cut(s) 133
Bsh1236I CGCG 3 cut(s) 1782, 2231, 2873
Bsh1285I CGRYCG 1 cut(s) 67
BshFI GGCC 7 cut(s) 374, 393, 507, 1084, 1617, 2254, 2692
BsiEI CGRYCG 1 cut(s) 67
BsiHKCI CYCGRG 1 cut(s) 369
BsiSI CCGG 2 cut(s) 1955, 2408
BslFI GGGAC 6 cut(s) 495, 2173, 2506, 2569, 2695, 2912
BslI CCNNNNNNNGG 9 cut(s) 102, 518, 1676, 1860, 1876, 2179, 2250, 2302, 2512
BsmAI GTCTC 5 cut(s) 78, 90, 554, 2451, 2873
BsmBI CGTCTC 1 cut(s) 90
BsmFI GGGAC 6 cut(s) 495, 2173, 2506, 2569, 2695, 2912
BsmI GAATGC 1 cut(s) 2758
BsnI GGCC 7 cut(s) 374, 393, 507, 1084, 1617, 2254, 2692
Bso31I GGTCTC 1 cut(s) 554
BsoBI CYCGRG 1 cut(s) 369
Bsp119I TTCGAA 1 cut(s) 2284
Bsp120I GGGCCC 1 cut(s) 372
Bsp1286I GDGCHC 3 cut(s) 376, 1870, 2665
Bsp1407I TGTACA 1 cut(s) 2322
Bsp143I GATC 8 cut(s) 952, 1123, 1357, 1762, 1783, 2053, 2479, 2740
Bsp19I CCATGG 3 cut(s) 1985, 2191, 2893
BspANI GGCC 7 cut(s) 374, 393, 507, 1084, 1617, 2254, 2692
BspFNI CGCG 3 cut(s) 1782, 2231, 2873
BspHI TCATGA 1 cut(s) 2856
BspMAI CTGCAG 1 cut(s) 2748
BspMI ACCTGC 1 cut(s) 373
BspPI GGATC 3 cut(s) 947, 1118, 2487
BspQI GCTCTTC 1 cut(s) 605
BspT104I TTCGAA 1 cut(s) 2284
BspTNI GGTCTC 1 cut(s) 554
BsrDI GCAATG 1 cut(s) 2031
BsrGI TGTACA 1 cut(s) 2322
BsrI ACTGG 7 cut(s) 293, 702, 934, 1085, 1145, 1855, 2481
BssMI GATC 8 cut(s) 952, 1123, 1357, 1762, 1783, 2053, 2479, 2740
BssNI GRCGYC 1 cut(s) 757
BssSI CACGAG 2 cut(s) 2041, 2686
BssT1I CCWWGG 9 cut(s) 394, 402, 1985, 2154, 2173, 2191, 2298, 2636, 2893
Bst2BI CACGAG 2 cut(s) 2041, 2686
Bst2UI CCWGG 9 cut(s) 720, 1028, 1080, 1923, 2202, 2245, 2507, 2604, 2941
Bst4CI ACNGT 7 cut(s) 625, 1306, 1365, 1911, 1935, 2028, 2853
Bst6I CTCTTC 3 cut(s) 114, 605, 1839
BstACI GRCGYC 1 cut(s) 757
BstAPI GCANNNNNTGC 1 cut(s) 272
BstAUI TGTACA 1 cut(s) 2322
BstBAI YACGTR 1 cut(s) 2773
BstBI TTCGAA 1 cut(s) 2284
BstC8I GCNNGC 5 cut(s) 324, 1756, 1866, 2497, 2900
BstDSI CCRYGG 4 cut(s) 1985, 2191, 2229, 2893
BstENI CCTNNNNNAGG 1 cut(s) 2510
BstFNI CGCG 3 cut(s) 1782, 2231, 2873
BstH2I RGCGCY 1 cut(s) 1684
BstHHI GCGC 1 cut(s) 1683
BstKTI GATC 8 cut(s) 955, 1126, 1360, 1765, 1786, 2056, 2482, 2743
BstMAI GTCTC 5 cut(s) 78, 90, 554, 2451, 2873
BstMBI GATC 8 cut(s) 952, 1123, 1357, 1762, 1783, 2053, 2479, 2740
BstMCI CGRYCG 1 cut(s) 67
BstMWI GCNNNNNNNGC 5 cut(s) 263, 272, 380, 2326, 2831
BstNI CCWGG 9 cut(s) 720, 1028, 1080, 1923, 2202, 2245, 2507, 2604, 2941
BstNSI RCATGY 2 cut(s) 905, 2499
BstSCI CCNGG 9 cut(s) 718, 1026, 1078, 1921, 2200, 2243, 2505, 2602, 2939
BstSFI CTRYAG 2 cut(s) 2269, 2744
BstSLI GKGCMC 3 cut(s) 376, 1870, 2665
BstUI CGCG 3 cut(s) 1782, 2231, 2873
BstV1I GCAGC 5 cut(s) 787, 1938, 2355, 2821, 2922
BstV2I GAAGAC 1 cut(s) 2874
BstX2I RGATCY 1 cut(s) 1123
BstYI RGATCY 1 cut(s) 1123
BsuRI GGCC 7 cut(s) 374, 393, 507, 1084, 1617, 2254, 2692
BtgI CCRYGG 4 cut(s) 1985, 2191, 2229, 2893
BtsI GCAGTG 1 cut(s) 2255
BtsIMutI CAGTG 6 cut(s) 135, 709, 1092, 1302, 2033, 2255
BveI ACCTGC 1 cut(s) 373
Cac8I GCNNGC 5 cut(s) 324, 1756, 1866, 2497, 2900
CciI TCATGA 1 cut(s) 2856
CfoI GCGC 1 cut(s) 1683
Cfr42I CCGCGG 1 cut(s) 2232
CseI GACGC 3 cut(s) 746, 2862, 2879
Csp6I GTAC 6 cut(s) 124, 1204, 2216, 2323, 2776, 2787
CviQI GTAC 6 cut(s) 124, 1204, 2216, 2323, 2776, 2787
DpnI GATC 8 cut(s) 954, 1125, 1359, 1764, 1785, 2055, 2481, 2742
DpnII GATC 8 cut(s) 952, 1123, 1357, 1762, 1783, 2053, 2479, 2740
DraI TTTAAA 1 cut(s) 2106
DraIII CACNNNGTG 1 cut(s) 1760
DrdI GACNNNNNNGTC 1 cut(s) 568
DseDI GACNNNNNNGTC 1 cut(s) 568
EaeI YGGCCR 2 cut(s) 391, 505
Eam1104I CTCTTC 3 cut(s) 114, 605, 1839
EarI CTCTTC 3 cut(s) 114, 605, 1839
Eco130I CCWWGG 9 cut(s) 394, 402, 1985, 2154, 2173, 2191, 2298, 2636, 2893
Eco147I AGGCCT 1 cut(s) 2692
Eco24I GRGCYC 1 cut(s) 376
Eco31I GGTCTC 1 cut(s) 554
Eco32I GATATC 3 cut(s) 886, 1212, 2262
Eco47I GGWCC 6 cut(s) 716, 1020, 1982, 2410, 2520, 2682
Eco88I CYCGRG 1 cut(s) 369
EcoNI CCTNNNNNAGG 1 cut(s) 2510
EcoO109I RGGNCCY 1 cut(s) 373
EcoRI GAATTC 1 cut(s) 856
EcoRII CCWGG 9 cut(s) 718, 1026, 1078, 1921, 2200, 2243, 2505, 2602, 2939
EcoRV GATATC 3 cut(s) 886, 1212, 2262
EcoT14I CCWWGG 9 cut(s) 394, 402, 1985, 2154, 2173, 2191, 2298, 2636, 2893
EcoT22I ATGCAT 1 cut(s) 907
EcoT38I GRGCYC 1 cut(s) 376
ErhI CCWWGG 9 cut(s) 394, 402, 1985, 2154, 2173, 2191, 2298, 2636, 2893
Esp3I CGTCTC 1 cut(s) 90
FaqI GGGAC 6 cut(s) 495, 2173, 2506, 2569, 2695, 2912
FauI CCCGC 2 cut(s) 2662, 2891
FbaI TGATCA 1 cut(s) 2053
Fnu4HI GCNGC 6 cut(s) 801, 1952, 2229, 2369, 2835, 2936
FriOI GRGCYC 1 cut(s) 376
Fsp4HI GCNGC 6 cut(s) 801, 1952, 2229, 2369, 2835, 2936
FspBI CTAG 4 cut(s) 384, 926, 1860, 2753
GlaI GCGC 1 cut(s) 1682
GluI GCNGC 6 cut(s) 801, 1952, 2229, 2369, 2835, 2936
GsaI CCCAGC 1 cut(s) 137
GsuI CTGGAG 2 cut(s) 2227, 2934
HaeII RGCGCY 1 cut(s) 1684
HaeIII GGCC 7 cut(s) 374, 393, 507, 1084, 1617, 2254, 2692
HapII CCGG 2 cut(s) 1955, 2408
HgaI GACGC 3 cut(s) 746, 2862, 2879
HhaI GCGC 1 cut(s) 1683
Hin1I GRCGYC 1 cut(s) 757
Hin6I GCGC 1 cut(s) 1681
HinP1I GCGC 1 cut(s) 1681
HincII GTYRAC 1 cut(s) 1744
HindII GTYRAC 1 cut(s) 1744
HindIII AAGCTT 3 cut(s) 867, 1637, 2093
HinfI GANTC 8 cut(s) 167, 724, 848, 1002, 1849, 1999, 2315, 2444
HpaII CCGG 2 cut(s) 1955, 2408
HphI GGTGA 5 cut(s) 1292, 1811, 2190, 2548, 2624
Hpy166II GTNNAC 7 cut(s) 445, 621, 707, 1093, 1744, 2198, 2776
Hpy188I TCNGA 8 cut(s) 574, 853, 1536, 2212, 2338, 2550, 2844, 2878
Hpy188III TCNNGA 8 cut(s) 344, 863, 881, 1165, 1199, 1215, 2375, 2857
Hpy8I GTNNAC 7 cut(s) 445, 621, 707, 1093, 1744, 2198, 2776
Hpy99I CGWCG 1 cut(s) 2723
HpyAV CCTTC 8 cut(s) 1141, 1267, 1271, 1472, 1607, 2145, 2229, 2941
HpyCH4III ACNGT 7 cut(s) 625, 1306, 1365, 1911, 1935, 2028, 2853
HpyCH4IV ACGT 6 cut(s) 447, 1390, 1438, 2595, 2721, 2772
HpyF10VI GCNNNNNNNGC 5 cut(s) 263, 272, 380, 2326, 2831
HpySE526I ACGT 6 cut(s) 447, 1390, 1438, 2595, 2721, 2772
Hsp92I GRCGYC 1 cut(s) 757
HspAI GCGC 1 cut(s) 1681
Ksp22I TGATCA 1 cut(s) 2053
KspI CCGCGG 1 cut(s) 2232
Kzo9I GATC 8 cut(s) 952, 1123, 1357, 1762, 1783, 2053, 2479, 2740
LguI GCTCTTC 1 cut(s) 605
LmnI GCTCC 8 cut(s) 200, 254, 773, 1831, 2041, 2164, 2246, 2915
Lsp1109I GCAGC 5 cut(s) 787, 1938, 2355, 2821, 2922
LweI GCATC 7 cut(s) 262, 820, 908, 914, 1276, 1543, 2285
MaeI CTAG 4 cut(s) 384, 926, 1860, 2753
MaeII ACGT 6 cut(s) 447, 1390, 1438, 2595, 2721, 2772
MalI GATC 8 cut(s) 954, 1125, 1359, 1764, 1785, 2055, 2481, 2742
MboI GATC 8 cut(s) 952, 1123, 1357, 1762, 1783, 2053, 2479, 2740
MfeI CAATTG 3 cut(s) 822, 1587, 2223
MflI RGATCY 1 cut(s) 1123
MhlI GDGCHC 3 cut(s) 376, 1870, 2665
MlsI TGGCCA 1 cut(s) 393
MluI ACGCGT 1 cut(s) 2871
MluNI TGGCCA 1 cut(s) 393
MlyI GAGTC 3 cut(s) 733, 2309, 2453
MmeI TCCRAC 2 cut(s) 955, 1156
Mox20I TGGCCA 1 cut(s) 393
Mph1103I ATGCAT 1 cut(s) 907
MscI TGGCCA 1 cut(s) 393
MseI TTAA 7 cut(s) 354, 1323, 1371, 1641, 1815, 2078, 2105
MslI CAYNNNNRTG 3 cut(s) 480, 2703, 2843
Msp20I TGGCCA 1 cut(s) 393
MspA1I CMGCKG 3 cut(s) 2231, 2912, 2935
MspI CCGG 2 cut(s) 1955, 2408
MspR9I CCNGG 9 cut(s) 720, 1028, 1080, 1923, 2202, 2245, 2507, 2604, 2941
MunI CAATTG 3 cut(s) 822, 1587, 2223
Mva1269I GAATGC 1 cut(s) 2758
MvaI CCWGG 9 cut(s) 720, 1028, 1080, 1923, 2202, 2245, 2507, 2604, 2941
MvnI CGCG 3 cut(s) 1782, 2231, 2873
MwoI GCNNNNNNNGC 5 cut(s) 263, 272, 380, 2326, 2831
NcoI CCATGG 3 cut(s) 1985, 2191, 2893
NdeII GATC 8 cut(s) 952, 1123, 1357, 1762, 1783, 2053, 2479, 2740
NmeAIII GCCGAG 1 cut(s) 1262
NmuCI GTSAC 6 cut(s) 424, 737, 1300, 2020, 2630, 2847
NsiI ATGCAT 1 cut(s) 907
NspI RCATGY 2 cut(s) 905, 2499
NspV TTCGAA 1 cut(s) 2284
PaeI GCATGC 1 cut(s) 2499
PagI TCATGA 1 cut(s) 2856
PasI CCCWGGG 1 cut(s) 1027
PceI AGGCCT 1 cut(s) 2692
PciSI GCTCTTC 1 cut(s) 605
PctI GAATGC 1 cut(s) 2758
PfeI GAWTC 5 cut(s) 167, 848, 1002, 1849, 1999
PflMI CCANNNNNTGG 1 cut(s) 2250
PfoI TCCNGGA 3 cut(s) 1921, 2505, 2602
PkrI GCNGC 6 cut(s) 802, 1953, 2230, 2370, 2836, 2937
PleI GAGTC 3 cut(s) 732, 2309, 2452
PpsI GAGTC 3 cut(s) 732, 2309, 2452
Ppu21I YACGTR 1 cut(s) 2773
Psp1406I AACGTT 3 cut(s) 447, 1438, 2595
Psp6I CCWGG 9 cut(s) 718, 1026, 1078, 1921, 2200, 2243, 2505, 2602, 2939
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 9 cut(s) 718, 1026, 1078, 1921, 2200, 2243, 2505, 2602, 2939
PspOMI GGGCCC 1 cut(s) 372
PsrI GAACNNNNNNTAC 2 cut(s) 1551, 1583
PstI CTGCAG 1 cut(s) 2748
PsuI RGATCY 1 cut(s) 1123
PvuII CAGCTG 1 cut(s) 2935
RsaI GTAC 6 cut(s) 125, 1205, 2217, 2324, 2777, 2788
RsaNI GTAC 6 cut(s) 124, 1204, 2216, 2323, 2776, 2787
RseI CAYNNNNRTG 3 cut(s) 480, 2703, 2843
SacII CCGCGG 1 cut(s) 2232
SapI GCTCTTC 1 cut(s) 605
SaqAI TTAA 7 cut(s) 354, 1323, 1371, 1641, 1815, 2078, 2105
SatI GCNGC 6 cut(s) 801, 1952, 2229, 2369, 2835, 2936
Sau3AI GATC 8 cut(s) 952, 1123, 1357, 1762, 1783, 2053, 2479, 2740
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 3 cut(s) 733, 2309, 2453
ScrFI CCNGG 9 cut(s) 720, 1028, 1080, 1923, 2202, 2245, 2507, 2604, 2941
SduI GDGCHC 3 cut(s) 376, 1870, 2665
SfaNI GCATC 7 cut(s) 262, 820, 908, 914, 1276, 1543, 2285
SfcI CTRYAG 2 cut(s) 2269, 2744
Sfr303I CCGCGG 1 cut(s) 2232
SfuI TTCGAA 1 cut(s) 2284
SgrBI CCGCGG 1 cut(s) 2232
SinI GGWCC 6 cut(s) 716, 1020, 1982, 2410, 2520, 2682
SmiMI CAYNNNNRTG 3 cut(s) 480, 2703, 2843
SmlI CTYRAG 5 cut(s) 861, 1163, 1199, 1409, 1605
SmoI CTYRAG 5 cut(s) 861, 1163, 1199, 1409, 1605
SpeI ACTAGT 1 cut(s) 2752
SphI GCATGC 1 cut(s) 2499
SseBI AGGCCT 1 cut(s) 2692
SspI AATATT 3 cut(s) 457, 1048, 2129
SspMI CTAG 4 cut(s) 384, 926, 1860, 2753
StuI AGGCCT 1 cut(s) 2692
StyD4I CCNGG 9 cut(s) 718, 1026, 1078, 1921, 2200, 2243, 2505, 2602, 2939
StyI CCWWGG 9 cut(s) 394, 402, 1985, 2154, 2173, 2191, 2298, 2636, 2893
TaaI ACNGT 7 cut(s) 625, 1306, 1365, 1911, 1935, 2028, 2853
TaiI ACGT 6 cut(s) 450, 1393, 1441, 2598, 2724, 2775
TaqI TCGA 4 cut(s) 1058, 1672, 1821, 2284
TaqII GACCGA 2 cut(s) 53, 756
TatI WGTACW 3 cut(s) 123, 1203, 2322
TauI GCSGC 1 cut(s) 2231
TfiI GAWTC 5 cut(s) 167, 848, 1002, 1849, 1999
Tru1I TTAA 7 cut(s) 354, 1323, 1371, 1641, 1815, 2078, 2105
Tru9I TTAA 7 cut(s) 354, 1323, 1371, 1641, 1815, 2078, 2105
TscAI CASTG 6 cut(s) 135, 709, 1092, 1309, 2033, 2255
TseFI GTSAC 6 cut(s) 424, 737, 1300, 2020, 2630, 2847
TseI GCWGC 5 cut(s) 800, 1951, 2368, 2834, 2935
Tsp45I GTSAC 6 cut(s) 424, 737, 1300, 2020, 2630, 2847
TspDTI ATGAA 9 cut(s) 159, 263, 513, 830, 1820, 2100, 2757, 2845, 2873
TspGWI ACGGA 3 cut(s) 1116, 1668, 2791
TspRI CASTG 6 cut(s) 135, 709, 1092, 1309, 2033, 2255
Van91I CCANNNNNTGG 1 cut(s) 2250
VpaK11BI GGWCC 6 cut(s) 716, 1020, 1982, 2410, 2520, 2682
XagI CCTNNNNNAGG 1 cut(s) 2510
XceI RCATGY 2 cut(s) 905, 2499
XcmI CCANNNNNNNNNTGG 1 cut(s) 2198
XspI CTAG 4 cut(s) 384, 926, 1860, 2753
ZrmI AGTACT 1 cut(s) 125
Zsp2I ATGCAT 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.