MD03G1164000.v1.1

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006994
Physical Location & Seq
Forward (+)
21222343 .. 21231055
8713 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1164000.v1.1.491

Sequence Viewer

Length: 2826 bp
ATGGATATGGATTCGTTTGAGCCAGATGGAAATGCAACAAATGGGGTGAATGGAGCTAATGGGGCAAATGTAGTGAATGGAGCAAATGGGGTGAATGGAGCTAATGGTGCAGAGGATGCGTTGCCACCCCCACCCCCAGTTATTCCACCAGATGTTGTTCCTTTGCATGTGGAAGCAGATAACATTCCTGAACCTGTTAAGAAAAAACCTGCTCGGGTCCCTATTGCTAGGCGTGGCCTTGGAACCAAGGGAACAAAAATACCCTTGGTCACAAATCACTTCAAAGTGAATGTTACTAATATTGAGGGTTACTTCTTCCATTATAGTGTTTCTGTCGCTTATGAAGATGGGCGTCCGCTTGACGGCAAGGGTGCTGGAAGAAGAATAATTGAAAGGGTGCATGAGACATACCACTCGGAGTTAGGTGGAAAGGACTTTGCCTATGATGGGGAGAAGAGCCTGTTTACTGTTGGATCCCTTCCGCGCAACAAACTTGAATTCGCCGTTGTTTTGGAGGATGTGCCATCAAGCAGGAACAATGGAAACTGTGAACCTGATGGTCCTGGGAGTCCAAATGAGAGTGACCGAAAGAGATTACGGCGTCCAAATCGCTCCAAAACATTTAATGTGGAGATCAGCTATGCTGCAAAAATTCCCATGAAAGCAATTGGAGATGCTCTTCGTGGTCAAGAATCAGAGAATTCTCAAGAAGCTTTGAGAGTCCTGGATATCATATTAAGACAGCATGCATCGAAGCAAGGATGCCTCCTAGTTCGCCAATCATTCTTCCATAATGATCCAAAAAATTTTGCGGATGTTGGAGGTGGTGTTCTTGGGTGCAGAGGATTCCATTCAAGTTTTAGGACCACCCAGGGTGGCTTGTCTCTGAACATTGATGTATCGACTACCATGATCATACAGCCAGGGCCGGTGGTAGACTTTTTAATTGCCAACCAAAGTGTTAGAGATCCTTTCCAACTTGACTGGACGAAGGCTAAAAGAACACTCAAGAATTTGAGGGTCAAAACAAGTCCGTCAAATCAAGAGTTCAAAATAACTGGATTGAGCGAGAAGCCATGCCGAGAACAGACTTTTACATTGAGAAACAAGCAAGCAAAGGAAGGGGAAGATGCAGAAATAGAAGTGACTGTTTATGATTATTTTGTTAATCATCGTAATATACAGCTGCGGTACTCGGCTGATCTACCCTGCCTTAATGTTGGGAAACCAAAACGTCCTACTTATATCCCCTTGGAGCTTTGTTCTTTGGTGTCCTTGCAACGTTACACAAAAGCTCTTTCCACCCTTCAAAGAGCTTCGCTGGTGGAGAAATCAAGGCAGAAGCCACAAGAGAGGATGAGTGTTTTGTCTAATGCTTTGAAAATCAATAATTATGATGCTGAACCAATGCTCCACTCATGTGGTGTTTCAATTGGTGGTAGCTTTACTCAAGTGGAAGGCCGTGTTCTTCCAGCCCCAAAGCTTAAAGTTGGTAATGGTGATGATTTCTTCCCTCGAAATGGGCGCTGGAATTTCAACAACAAGACACTTGTCAAGCCAACTAAGATAGAAAAGTGGGCTGTTGTTAACTTCTCTGCACGCTGTGATCTAAAAGCCCTCGTCCGTGATCTGATCAAATGTGGAGATATGAAAGGAATTAAAATCGAGGCTCCATTTGATGTGTTTGAAGAGAATCCTCAGTCTAGGCGCGCCCCACCTTTGGTTAGAGTTGAGAGGATGTTTGAGGATATACAGTCCAAACTTCCTGGACAACCACAGTTTCTTCTCTGTTTGCTTCCGGAGAGAAAAAATTCTGCTTTATATGGTCCATGGAAGCGGAAGAATCTGGCTGAGTATGGTATTGTGACACAATGCATTGCTCCCACAAGGGTCAATGATCAATACCTTACAAATGTCCTGTTAAAGATCAATGCAAAGCTTGGTGGGTTAAATTCATTGTTGGCAGTTGAATATTCTCCTTCTATCCCTGTGGTTTCCAAGGCTCCCACTATCATCCTTGGGATGGATGTGTCCCATGGTTCTCCTGGGCAATCTGATGTACCCTCAATTGCTGCGGTGGTAAGCTCCAGGCAGTGGCCACTGATTTCTCGCTACAGGGCATCTGTTCGAACACAATCCCCCAAGGTGGAGATGATAGACTCTCTGTACAAACGAGTTTCTGACACAGAGGATGATGGCATTATGAGAGAGCTGCTTCTGGACTTCTATACAAGTTCAGGCAAACAGAAACCTGACCAGATTATCATCTTCAGGGATGGAGTAAGCGAATCTCAATTTAACCAAGTTTTGAACATTGAACTGGATCAAATCATAGAGGCTTGCAAGTTCCTGGATGAGACTTGGTCCCCTAAATTTGTGGTGATCATTGCTCAGAAGAACCACCACACAAAATTTTTTCAGCCTCAATCCCCAGATAATGTTCCTCCTGGAACAATTATCGACAACAAAATTTGTCACCCAAGAAACAATGACTTCTATCTATGTGCTCAAGCTGGAATGATTGGGACCACGAGGCCTACTCACTATCATGTTCTGTTAGATGATGTTGGTTTTTCAGCCGATGAGCTGCAGGAACTAGTGCATTCACTTTCATACGTGTACCAGAGAAGTACCACCGCCATTTCCGTAGTTGCCCCCATTTGCTATGCACATTTAGCTGCCACTCAGATGTCACAGTTCATGAAATTCGAAGACGCGTCTGAGACATCTTCAAGCCATGGCGGTCTTACCTCTGCTGGAGCTGTTCCTGTCCCGCAGCTGCCGAGGTTGAAGGAGAATGTGTCCAGTTCCATGTTTTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003682 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0008150 GO:0008152 GO:0008213 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009814 GO:0009816 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015030 GO:0016032 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016604 GO:0016787 GO:0016788 GO:0018022 GO:0018193 GO:0018205 GO:0019048 GO:0019219 GO:0019222 GO:0019538 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0034641 GO:0034968 GO:0035194 GO:0035197 GO:0035198 GO:0035821 GO:0036211 GO:0040029 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044728 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0060255 GO:0061647 GO:0061980 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0140098 GO:1901360 GO:1901363 GO:1901564 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

942

Amino Acids

104.29

Weight (kDa)

8.74

Isoelectric Point (pI)

47.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 85 - 246 3.4e-30 N-terminal domain of argonaute
ArgoL1 PF08699 258 - 307 5.4e-19 Argonaute linker 1 domain
PAZ PF02170 312 - 441 1e-31 PAZ domain
ArgoL2 PF16488 451 - 496 2.3e-11 Argonaute linker 2 domain
ArgoMid PF16487 505 - 557 9.3e-07 Mid domain of argonaute
Piwi PF02171 595 - 902 6.2e-106 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 217
AccB7I CCANNNNNTGG 1 cut(s) 2094
AccI GTMKAC 1 cut(s) 936
AccII CGCG 3 cut(s) 484, 1710, 2717
AccIII TCCGGA 1 cut(s) 1798
AciI CCGC 9 cut(s) 356, 482, 812, 1189, 1837, 2075, 2637, 2742, 2774
AclI AACGTT 1 cut(s) 1282
AclWI GGATC 5 cut(s) 468, 481, 791, 962, 2331
AcoI YGGCCR 1 cut(s) 2096
AcuI CTGAAG 1 cut(s) 2254
AcyI GRCGYC 2 cut(s) 352, 601
AdeI CACNNNGTG 1 cut(s) 1604
AfaI GTAC 5 cut(s) 1193, 2061, 2168, 2621, 2632
AfiI CCNNNNNNNGG 7 cut(s) 362, 447, 1520, 1720, 2023, 2094, 2146
AflIII ACRYGT 2 cut(s) 2616, 2715
AhlI ACTAGT 1 cut(s) 2596
AjnI CCWGG 9 cut(s) 562, 723, 870, 922, 1765, 2044, 2087, 2349, 2446
AleI CACNNNNGTG 1 cut(s) 1419
AloI GAACNNNNNNTCC 4 cut(s) 813, 845, 1395, 1427
Alw21I GWGCWC 1 cut(s) 2509
Alw26I GTCTC 4 cut(s) 398, 888, 2351, 2717
AlwI GGATC 5 cut(s) 468, 481, 791, 962, 2331
Ama87I CYCGRG 1 cut(s) 213
Aor13HI TCCGGA 1 cut(s) 1798
AoxI GGCC 5 cut(s) 235, 926, 1459, 2096, 2534
ApeKI GCWGC 8 cut(s) 644, 1186, 2072, 2212, 2587, 2678, 2776, 2779
AscI GGCGCGCC 1 cut(s) 1708
Asp700I GAANNNNTTC 1 cut(s) 1810
AspLEI GCGC 4 cut(s) 486, 1527, 1710, 1712
AspS9I GGNCC 7 cut(s) 217, 560, 864, 926, 1826, 2364, 2526
AsuHPI GGTGA 5 cut(s) 58, 103, 1511, 2392, 2468
AsuII TTCGAA 2 cut(s) 2128, 2709
AvaI CYCGRG 1 cut(s) 213
AvaII GGWCC 6 cut(s) 217, 560, 864, 1826, 2364, 2526
BalI TGGCCA 1 cut(s) 2098
BamHI GGATCC 1 cut(s) 473
BauI CACGAG 1 cut(s) 2530
BbsI GAAGAC 1 cut(s) 2718
Bbv12I GWGCWC 1 cut(s) 2509
BbvI GCAGC 8 cut(s) 631, 1173, 2059, 2199, 2574, 2665, 2766, 2788
BccI CCATC 8 cut(s) 20, 341, 440, 532, 551, 2017, 2189, 2270
BceAI ACGGC 4 cut(s) 379, 488, 614, 1446
BciT130I CCWGG 9 cut(s) 564, 725, 872, 924, 1767, 2046, 2089, 2351, 2448
BclI TGATCA 4 cut(s) 912, 1632, 1897, 2382
BcoDI GTCTC 4 cut(s) 398, 888, 2351, 2717
BcuI ACTAGT 1 cut(s) 2596
BfaI CTAG 4 cut(s) 228, 770, 1704, 2597
BfmI CTRYAG 2 cut(s) 2113, 2588
BfoI RGCGCY 1 cut(s) 1528
BfuAI ACCTGC 1 cut(s) 217
BisI GCNGC 8 cut(s) 645, 1187, 2073, 2213, 2588, 2679, 2777, 2780
BlsI GCNGC 8 cut(s) 646, 1188, 2074, 2214, 2589, 2680, 2778, 2781
Bme1390I CCNGG 9 cut(s) 564, 725, 872, 924, 1767, 2046, 2089, 2351, 2448
Bme18I GGWCC 6 cut(s) 217, 560, 864, 1826, 2364, 2526
BmeT110I CYCGRG 1 cut(s) 213
BmgT120I GGNCC 7 cut(s) 217, 560, 864, 926, 1826, 2364, 2526
BmiI GGNNCC 8 cut(s) 218, 219, 244, 475, 1671, 2004, 2366, 2527
BmrFI CCNGG 9 cut(s) 564, 725, 872, 924, 1767, 2046, 2089, 2351, 2448
BmrI ACTGGG 1 cut(s) 131
BmsI GCATC 7 cut(s) 106, 664, 752, 758, 1120, 1387, 2129
BmuI ACTGGG 1 cut(s) 131
BoxI GACNNNNGTC 1 cut(s) 1550
BpiI GAAGAC 1 cut(s) 2718
BplI GAGNNNNNCTC 2 cut(s) 2524, 2556
BpmI CTGGAG 2 cut(s) 2071, 2778
Bpu14I TTCGAA 2 cut(s) 2128, 2709
BpuEI CTTGAG 4 cut(s) 690, 992, 1434, 2493
BsaAI YACGTR 1 cut(s) 2617
BsaBI GATNNNNATC 1 cut(s) 2264
BsaHI GRCGYC 2 cut(s) 352, 601
BsaWI WCCGGW 1 cut(s) 1798
BsaXI ACNNNNNCTCC 6 cut(s) 813, 843, 1395, 1425, 2786, 2816
Bsc4I CCNNNNNNNGG 7 cut(s) 362, 447, 1520, 1720, 2023, 2094, 2146
Bse118I RCCGGY 1 cut(s) 928
Bse1I ACTGG 5 cut(s) 137, 989, 1063, 2325, 2805
Bse3DI GCAATG 2 cut(s) 1875, 2385
Bse8I GATNNNNATC 1 cut(s) 2264
BseAI TCCGGA 1 cut(s) 1798
BseBI CCWGG 9 cut(s) 564, 725, 872, 924, 1767, 2046, 2089, 2351, 2448
BseJI GATNNNNATC 1 cut(s) 2264
BseLI CCNNNNNNNGG 7 cut(s) 362, 447, 1520, 1720, 2023, 2094, 2146
BseMI GCAATG 2 cut(s) 1875, 2385
BseMII CTCAG 5 cut(s) 1712, 1842, 2405, 2699, 2712
BseNI ACTGG 5 cut(s) 137, 989, 1063, 2325, 2805
BsePI GCGCGC 1 cut(s) 1708
BseXI GCAGC 8 cut(s) 631, 1173, 2059, 2199, 2574, 2665, 2766, 2788
BsgI GTGCAG 3 cut(s) 129, 859, 1581
Bsh1236I CGCG 3 cut(s) 484, 1710, 2717
BshFI GGCC 5 cut(s) 237, 928, 1461, 2098, 2536
BsiHKAI GWGCWC 1 cut(s) 2509
BsiHKCI CYCGRG 1 cut(s) 213
BsiSI CCGG 2 cut(s) 929, 1799
BslFI GGGAC 5 cut(s) 203, 2017, 2350, 2539, 2756
BslI CCNNNNNNNGG 7 cut(s) 362, 447, 1520, 1720, 2023, 2094, 2146
BsmAI GTCTC 4 cut(s) 398, 888, 2351, 2717
BsmFI GGGAC 5 cut(s) 203, 2017, 2350, 2539, 2756
BsmI GAATGC 1 cut(s) 2602
BsnI GGCC 5 cut(s) 237, 928, 1461, 2098, 2536
BsoBI CYCGRG 1 cut(s) 213
Bsp119I TTCGAA 2 cut(s) 2128, 2709
Bsp1286I GDGCHC 1 cut(s) 2509
Bsp13I TCCGGA 1 cut(s) 1798
Bsp1407I TGTACA 1 cut(s) 2166
Bsp19I CCATGG 3 cut(s) 1829, 2035, 2737
BspACI CCGC 9 cut(s) 356, 482, 812, 1189, 1837, 2075, 2637, 2742, 2774
BspANI GGCC 5 cut(s) 237, 928, 1461, 2098, 2536
BspCNI CTCAG 5 cut(s) 1711, 1843, 2404, 2698, 2713
BspEI TCCGGA 1 cut(s) 1798
BspFNI CGCG 3 cut(s) 484, 1710, 2717
BspHI TCATGA 1 cut(s) 2700
BspLI GGNNCC 8 cut(s) 218, 219, 244, 475, 1671, 2004, 2366, 2527
BspMAI CTGCAG 1 cut(s) 2592
BspMI ACCTGC 1 cut(s) 217
BspPI GGATC 5 cut(s) 468, 481, 791, 962, 2331
BspQI GCTCTTC 2 cut(s) 449, 684
BspT104I TTCGAA 2 cut(s) 2128, 2709
BsrDI GCAATG 2 cut(s) 1875, 2385
BsrFI RCCGGY 1 cut(s) 928
BsrGI TGTACA 1 cut(s) 2166
BsrI ACTGG 5 cut(s) 137, 989, 1063, 2325, 2805
BssAI RCCGGY 1 cut(s) 928
BssHII GCGCGC 1 cut(s) 1708
BssNI GRCGYC 2 cut(s) 352, 601
BssSI CACGAG 1 cut(s) 2530
Bst2BI CACGAG 1 cut(s) 2530
Bst2UI CCWGG 9 cut(s) 564, 725, 872, 924, 1767, 2046, 2089, 2351, 2448
Bst4CI ACNGT 6 cut(s) 469, 548, 1150, 1755, 1779, 2697
Bst6I CTCTTC 3 cut(s) 449, 684, 1683
BstACI GRCGYC 2 cut(s) 352, 601
BstAPI GCANNNNNTGC 1 cut(s) 116
BstAUI TGTACA 1 cut(s) 2166
BstBAI YACGTR 1 cut(s) 2617
BstBI TTCGAA 2 cut(s) 2128, 2709
BstC8I GCNNGC 5 cut(s) 747, 1113, 1600, 1710, 2341
BstDEI CTNAG 6 cut(s) 1563, 1698, 1851, 2391, 2685, 2721
BstDSI CCRYGG 3 cut(s) 1829, 2035, 2737
BstFNI CGCG 3 cut(s) 484, 1710, 2717
BstH2I RGCGCY 1 cut(s) 1528
BstHHI GCGC 4 cut(s) 486, 1527, 1710, 1712
BstMAI GTCTC 4 cut(s) 398, 888, 2351, 2717
BstMWI GCNNNNNNNGC 4 cut(s) 62, 107, 116, 2675
BstNI CCWGG 9 cut(s) 564, 725, 872, 924, 1767, 2046, 2089, 2351, 2448
BstNSI RCATGY 2 cut(s) 170, 749
BstPAI GACNNNNGTC 1 cut(s) 1550
BstSCI CCNGG 9 cut(s) 562, 723, 870, 922, 1765, 2044, 2087, 2349, 2446
BstSFI CTRYAG 2 cut(s) 2113, 2588
BstUI CGCG 3 cut(s) 484, 1710, 2717
BstV1I GCAGC 8 cut(s) 631, 1173, 2059, 2199, 2574, 2665, 2766, 2788
BstV2I GAAGAC 1 cut(s) 2718
BstX2I RGATCY 2 cut(s) 473, 967
BstXI CCANNNNNNTGG 1 cut(s) 1421
BstYI RGATCY 2 cut(s) 473, 967
BsuRI GGCC 5 cut(s) 237, 928, 1461, 2098, 2536
BtgI CCRYGG 3 cut(s) 1829, 2035, 2737
BtsI GCAGTG 1 cut(s) 2099
BtsIMutI CAGTG 2 cut(s) 2099, 2099
BveI ACCTGC 1 cut(s) 217
Cac8I GCNNGC 5 cut(s) 747, 1113, 1600, 1710, 2341
CciI TCATGA 1 cut(s) 2700
CfoI GCGC 4 cut(s) 486, 1527, 1710, 1712
Cfr10I RCCGGY 1 cut(s) 928
Cfr13I GGNCC 7 cut(s) 217, 560, 864, 926, 1826, 2364, 2526
CseI GACGC 4 cut(s) 341, 590, 2706, 2723
Csp6I GTAC 5 cut(s) 1192, 2060, 2167, 2620, 2631
CviQI GTAC 5 cut(s) 1192, 2060, 2167, 2620, 2631
DdeI CTNAG 6 cut(s) 1563, 1698, 1851, 2391, 2685, 2721
DraIII CACNNNGTG 1 cut(s) 1604
EaeI YGGCCR 1 cut(s) 2096
Eam1104I CTCTTC 3 cut(s) 449, 684, 1683
EarI CTCTTC 3 cut(s) 449, 684, 1683
Eco147I AGGCCT 1 cut(s) 2536
Eco32I GATATC 1 cut(s) 730
Eco47I GGWCC 6 cut(s) 217, 560, 864, 1826, 2364, 2526
Eco57I CTGAAG 1 cut(s) 2254
Eco88I CYCGRG 1 cut(s) 213
EcoO109I RGGNCCY 1 cut(s) 217
EcoRI GAATTC 2 cut(s) 497, 700
EcoRII CCWGG 9 cut(s) 562, 723, 870, 922, 1765, 2044, 2087, 2349, 2446
EcoRV GATATC 1 cut(s) 730
EcoT22I ATGCAT 2 cut(s) 751, 1877
FaqI GGGAC 5 cut(s) 203, 2017, 2350, 2539, 2756
FauI CCCGC 1 cut(s) 2781
FbaI TGATCA 4 cut(s) 912, 1632, 1897, 2382
FblI GTMKAC 1 cut(s) 936
Fnu4HI GCNGC 8 cut(s) 645, 1187, 2073, 2213, 2588, 2679, 2777, 2780
Fsp4HI GCNGC 8 cut(s) 645, 1187, 2073, 2213, 2588, 2679, 2777, 2780
FspBI CTAG 4 cut(s) 228, 770, 1704, 2597
GlaI GCGC 4 cut(s) 485, 1526, 1709, 1711
GluI GCNGC 8 cut(s) 645, 1187, 2073, 2213, 2588, 2679, 2777, 2780
GsuI CTGGAG 2 cut(s) 2071, 2778
HaeII RGCGCY 1 cut(s) 1528
HaeIII GGCC 5 cut(s) 237, 928, 1461, 2098, 2536
HapII CCGG 2 cut(s) 929, 1799
HgaI GACGC 4 cut(s) 341, 590, 2706, 2723
HhaI GCGC 4 cut(s) 486, 1527, 1710, 1712
Hin1I GRCGYC 2 cut(s) 352, 601
Hin6I GCGC 4 cut(s) 484, 1525, 1708, 1710
HinP1I GCGC 4 cut(s) 484, 1525, 1708, 1710
HincII GTYRAC 1 cut(s) 1588
HindII GTYRAC 1 cut(s) 1588
HindIII AAGCTT 3 cut(s) 711, 1481, 1937
HinfI GANTC 9 cut(s) 11, 568, 692, 720, 846, 1693, 1843, 2159, 2288
HpaI GTTAAC 1 cut(s) 1588
HpaII CCGG 2 cut(s) 929, 1799
HphI GGTGA 5 cut(s) 58, 103, 1511, 2392, 2468
Hpy166II GTNNAC 5 cut(s) 465, 551, 937, 1588, 2620
Hpy188I TCNGA 9 cut(s) 418, 697, 888, 1632, 2056, 2182, 2394, 2688, 2722
Hpy188III TCNNGA 8 cut(s) 188, 689, 707, 1009, 1043, 1799, 2219, 2701
Hpy8I GTNNAC 5 cut(s) 465, 551, 937, 1588, 2620
HpyAV CCTTC 7 cut(s) 488, 985, 1115, 1316, 1451, 1989, 2785
HpyCH4III ACNGT 6 cut(s) 469, 548, 1150, 1755, 1779, 2697
HpyCH4IV ACGT 3 cut(s) 1234, 1282, 2616
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 107, 116, 2675
HpyF3I CTNAG 6 cut(s) 1563, 1698, 1851, 2391, 2685, 2721
HpySE526I ACGT 3 cut(s) 1234, 1282, 2616
Hsp92I GRCGYC 2 cut(s) 352, 601
HspAI GCGC 4 cut(s) 484, 1525, 1708, 1710
KflI GGGWCCC 1 cut(s) 217
Kpn2I TCCGGA 1 cut(s) 1798
Ksp22I TGATCA 4 cut(s) 912, 1632, 1897, 2382
KspAI GTTAAC 1 cut(s) 1588
LguI GCTCTTC 2 cut(s) 449, 684
Lsp1109I GCAGC 8 cut(s) 631, 1173, 2059, 2199, 2574, 2665, 2766, 2788
LweI GCATC 7 cut(s) 106, 664, 752, 758, 1120, 1387, 2129
MaeI CTAG 4 cut(s) 228, 770, 1704, 2597
MaeII ACGT 3 cut(s) 1234, 1282, 2616
MaeIII GTNAC 9 cut(s) 268, 292, 308, 581, 1144, 1283, 1864, 2474, 2691
MfeI CAATTG 3 cut(s) 666, 1431, 2067
MflI RGATCY 2 cut(s) 473, 967
MhlI GDGCHC 1 cut(s) 2509
MlsI TGGCCA 1 cut(s) 2098
MluI ACGCGT 1 cut(s) 2715
MluNI TGGCCA 1 cut(s) 2098
MlyI GAGTC 3 cut(s) 577, 729, 2153
MmeI TCCRAC 3 cut(s) 451, 799, 1000
Mox20I TGGCCA 1 cut(s) 2098
Mph1103I ATGCAT 2 cut(s) 751, 1877
MroI TCCGGA 1 cut(s) 1798
MroXI GAANNNNTTC 1 cut(s) 1810
MscI TGGCCA 1 cut(s) 2098
MslI CAYNNNNRTG 4 cut(s) 324, 1419, 2547, 2687
Msp20I TGGCCA 1 cut(s) 2098
MspA1I CMGCKG 2 cut(s) 1186, 2779
MspI CCGG 2 cut(s) 929, 1799
MspR9I CCNGG 9 cut(s) 564, 725, 872, 924, 1767, 2046, 2089, 2351, 2448
MunI CAATTG 3 cut(s) 666, 1431, 2067
Mva1269I GAATGC 1 cut(s) 2602
MvaI CCWGG 9 cut(s) 564, 725, 872, 924, 1767, 2046, 2089, 2351, 2448
MvnI CGCG 3 cut(s) 484, 1710, 2717
MwoI GCNNNNNNNGC 4 cut(s) 62, 107, 116, 2675
NcoI CCATGG 3 cut(s) 1829, 2035, 2737
NlaIV GGNNCC 8 cut(s) 218, 219, 244, 475, 1671, 2004, 2366, 2527
NmeAIII GCCGAG 3 cut(s) 1106, 1175, 2808
NmuCI GTSAC 6 cut(s) 268, 581, 1144, 1864, 2474, 2691
NsiI ATGCAT 2 cut(s) 751, 1877
NspI RCATGY 2 cut(s) 170, 749
NspV TTCGAA 2 cut(s) 2128, 2709
OliI CACNNNNGTG 1 cut(s) 1419
PaeI GCATGC 1 cut(s) 749
PagI TCATGA 1 cut(s) 2700
PalAI GGCGCGCC 1 cut(s) 1708
PasI CCCWGGG 1 cut(s) 871
PauI GCGCGC 1 cut(s) 1708
PceI AGGCCT 1 cut(s) 2536
PciSI GCTCTTC 2 cut(s) 449, 684
PctI GAATGC 1 cut(s) 2602
PdmI GAANNNNTTC 1 cut(s) 1810
PfeI GAWTC 6 cut(s) 11, 692, 846, 1693, 1843, 2288
PflFI GACNNNGTC 1 cut(s) 2362
PflMI CCANNNNNTGG 1 cut(s) 2094
PfoI TCCNGGA 4 cut(s) 723, 1765, 2349, 2446
PkrI GCNGC 8 cut(s) 646, 1188, 2074, 2214, 2589, 2680, 2778, 2781
PleI GAGTC 3 cut(s) 576, 728, 2153
PpsI GAGTC 3 cut(s) 576, 728, 2153
Ppu21I YACGTR 1 cut(s) 2617
PpuMI RGGWCCY 1 cut(s) 217
PshAI GACNNNNGTC 1 cut(s) 1550
Psp1406I AACGTT 1 cut(s) 1282
Psp5II RGGWCCY 1 cut(s) 217
Psp6I CCWGG 9 cut(s) 562, 723, 870, 922, 1765, 2044, 2087, 2349, 2446
PspGI CCWGG 9 cut(s) 562, 723, 870, 922, 1765, 2044, 2087, 2349, 2446
PspN4I GGNNCC 8 cut(s) 218, 219, 244, 475, 1671, 2004, 2366, 2527
PspPI GGNCC 7 cut(s) 217, 560, 864, 926, 1826, 2364, 2526
PspPPI RGGWCCY 1 cut(s) 217
PstI CTGCAG 1 cut(s) 2592
PsuI RGATCY 2 cut(s) 473, 967
PsyI GACNNNGTC 1 cut(s) 2362
PteI GCGCGC 1 cut(s) 1708
PvuII CAGCTG 2 cut(s) 1186, 2779
RsaI GTAC 5 cut(s) 1193, 2061, 2168, 2621, 2632
RsaNI GTAC 5 cut(s) 1192, 2060, 2167, 2620, 2631
RseI CAYNNNNRTG 4 cut(s) 324, 1419, 2547, 2687
SapI GCTCTTC 2 cut(s) 449, 684
SatI GCNGC 8 cut(s) 645, 1187, 2073, 2213, 2588, 2679, 2777, 2780
Sau96I GGNCC 7 cut(s) 217, 560, 864, 926, 1826, 2364, 2526
SchI GAGTC 3 cut(s) 577, 729, 2153
ScrFI CCNGG 9 cut(s) 564, 725, 872, 924, 1767, 2046, 2089, 2351, 2448
SduI GDGCHC 1 cut(s) 2509
SfaNI GCATC 7 cut(s) 106, 664, 752, 758, 1120, 1387, 2129
SfcI CTRYAG 2 cut(s) 2113, 2588
SfuI TTCGAA 2 cut(s) 2128, 2709
SgsI GGCGCGCC 1 cut(s) 1708
SinI GGWCC 6 cut(s) 217, 560, 864, 1826, 2364, 2526
SmiMI CAYNNNNRTG 4 cut(s) 324, 1419, 2547, 2687
SmlI CTYRAG 4 cut(s) 705, 1007, 1449, 2508
SmoI CTYRAG 4 cut(s) 705, 1007, 1449, 2508
SpeI ACTAGT 1 cut(s) 2596
SphI GCATGC 1 cut(s) 749
SseBI AGGCCT 1 cut(s) 2536
SsiI CCGC 9 cut(s) 356, 482, 812, 1189, 1837, 2075, 2637, 2742, 2774
SspI AATATT 2 cut(s) 301, 1973
SspMI CTAG 4 cut(s) 228, 770, 1704, 2597
StuI AGGCCT 1 cut(s) 2536
StyD4I CCNGG 9 cut(s) 562, 723, 870, 922, 1765, 2044, 2087, 2349, 2446
TaaI ACNGT 6 cut(s) 469, 548, 1150, 1755, 1779, 2697
TaiI ACGT 3 cut(s) 1237, 1285, 2619
TaqI TCGA 7 cut(s) 752, 902, 1516, 1665, 2128, 2460, 2709
TaqII GACCGA 1 cut(s) 600
TatI WGTACW 1 cut(s) 2166
TfiI GAWTC 6 cut(s) 11, 692, 846, 1693, 1843, 2288
TscAI CASTG 2 cut(s) 2099, 2106
TseFI GTSAC 6 cut(s) 268, 581, 1144, 1864, 2474, 2691
TseI GCWGC 8 cut(s) 644, 1186, 2072, 2212, 2587, 2678, 2776, 2779
Tsp45I GTSAC 6 cut(s) 268, 581, 1144, 1864, 2474, 2691
TspDTI ATGAA 7 cut(s) 357, 674, 1664, 1944, 2601, 2689, 2717
TspGWI ACGGA 3 cut(s) 1023, 1613, 2635
TspRI CASTG 2 cut(s) 2099, 2106
Tth111I GACNNNGTC 1 cut(s) 2362
Van91I CCANNNNNTGG 1 cut(s) 2094
VpaK11BI GGWCC 6 cut(s) 217, 560, 864, 1826, 2364, 2526
XceI RCATGY 2 cut(s) 170, 749
XcmI CCANNNNNNNNNTGG 1 cut(s) 2042
XmiI GTMKAC 1 cut(s) 936
XmnI GAANNNNTTC 1 cut(s) 1810
XspI CTAG 4 cut(s) 228, 770, 1704, 2597
Zsp2I ATGCAT 2 cut(s) 751, 1877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.