AT2G28150

Protein UPSTREAM OF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006878
Physical Location & Seq
Forward (+)
11999736 .. 12002738
3003 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G28150.2

Sequence Viewer

Length: 1623 bp
ATGGAAGCGAGGATGAAGAAGTATAGCAGAGAAGTGAGTCCAGAGAGAGCAAAAGTCTGGACTGAGAAATCACCAAAGTATCATCAAAAGATCAAGAAAGTTCAAATTGTTTATTACCTCTCCAAGAATCGTCAACTTGAGCATCCTCATTTCATGGAAGTGTTGATTTCTTCTCCAAATGGTTTATACCTAAGAGATGTTATAGAGAGGCTTAATGTTCTTAGAGGTAGAGGAATGGCTTCAATGTATTCTTGGTCTAGTAAGAGAAGCTATAGGAATGGTTTTGTTTGGCATGATTTATCAGAAGATGATTTGATTTTACCAGCTAATGGGAATGAGTATGTTCTTAAAGGTTCTGAGCTATTTGATGAATCCAATTCAGATCACTTTAGCCCAATTGTGAATTTAGCTACTCAAAACATGAAGCAGATAGTTGTGGAGCCACCATCATCTAGAAGTATGGATGATTCGTCTTCATCTTCGAGTATGAATAATGGTAAAGGAACTAATAAGCATTCTCATGAAGATGATGAGCTTTCTCCTCCAGCTCTTCGCTCTGTTTCATCTTCCGGTGTATCTCCTGATTCTCGAGACGCTAAGAACTCATCTTCCTGGTGTTTGGCTGAGTACAAAGTGTATAAGAGCGAGGGACTCGCAGATGCTTCTACTCAAACCGATGAAACTGTGAGTGGTCGTTCTAAAACACCTATAGAGACTTTTAGTAGAGGTGTATCAACTGATGAAGATGTGTCATCAGAGCCTGAAACCAGCGAGAATAACCTCGTGAGTGAGGCTTCGTGTGCGGGGAAGGAGAGGGAGAGTGCGGAAATATCAAGAAACTCTGTCTCGCCGCCTTTTTCCAATAGTGCTTCTTCGCTAGGAGGAAAGACTGATACTTTGGAGTCTCTAATAAGAGCTGATGTTAGTAAGATGAATAGTTTTAGGATTCTTGAACAAGAAGATGTTCGAATGCCCGCTATTCCGAGGCTTAGGGCCTCGAATATGCTGATGCAGTTGATATCTTGTGGTTCAATATCAGTCAAGGATAACAATTTCGGCCTTGTACCTACTTACAAACCGAAATTTGGACACTCGAAGTTTCCGTCTCCTTTTTTCTCCTCGTCGTTTATGATGGGAGATCTTGACCGTTTGTCGGAAACTCCGAGTCTAATGGGCCTGAGGATGGAGGAGAAAGAGTATTTTAGTGGGAGCTTAGTGGAGACTAAGCTACAGAAGAAGGATGCAGCGGATAGTAACGCTTCTCTTAAGCGTTCTTCATCCTACAATGGTGACAGGGCATCCAACCAAATGGGTGTAGCAGAGAATGGAGACTCAAAACCGGATAGTTCTAAAAATAATCCAAGCTCAAGAAAGGCGTCTTCTATTCTAGGGAAGCAACAACCACTTGTCTCCGAAAAGAGAAGAGACTCATCAGAAGATACTACCAAGAACATTCCATGTACCACTAAAACACATGATGCTTGCAGTAAAAGAATAACCGAGTCTTTAAGGAAGCCCGATTCATTCAGAGAAGACGAGGAGAGAGTTATCAAGATCGACGAAAGGCTTGCTTCAGGAGCACGAGTTAGAATCGAATCGAAAGTGCCTTCAGAAGAGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

540

Amino Acids

60.08

Weight (kDa)

6.8

Isoelectric Point (pI)

60.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 1 - 44 2.8e-22 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 329
AciI CCGC 5 cut(s) 803, 824, 851, 975, 1247
AcsI RAATTY 2 cut(s) 403, 1082
AcuI CTGAAG 2 cut(s) 1557, 1593
AcyI GRCGYC 1 cut(s) 1376
AfaI GTAC 3 cut(s) 629, 1065, 1462
AfiI CCNNNNNNNGG 4 cut(s) 329, 1085, 1153, 1183
AflII CTTAAG 1 cut(s) 1265
AgsI TTSAA 4 cut(s) 104, 243, 953, 1032
AhdI GACNNNNNGTC 1 cut(s) 1150
AjnI CCWGG 1 cut(s) 611
AloI GAACNNNNNNTCC 2 cut(s) 593, 625
Alw21I GWGCWC 1 cut(s) 1582
Alw26I GTCTC 9 cut(s) 585, 707, 850, 909, 1110, 1214, 1323, 1414, 1419
AlwNI CAGNNNCTG 1 cut(s) 761
Ama87I CYCGRG 1 cut(s) 588
AoxI GGCC 3 cut(s) 993, 1057, 1174
ApeKI GCWGC 1 cut(s) 1244
ApoI RAATTY 2 cut(s) 403, 1082
ArsI GACNNNNNNTTYG 4 cut(s) 880, 912, 1088, 1120
Asp700I GAANNNNTTC 2 cut(s) 238, 963
AspS9I GGNCC 2 cut(s) 993, 1174
AsuHPI GGTGA 2 cut(s) 63, 1301
AsuII TTCGAA 1 cut(s) 967
AvaI CYCGRG 1 cut(s) 588
AxyI CCTNAGG 1 cut(s) 1178
BaeI ACNNNNGTAYC 2 cut(s) 62, 95
BauI CACGAG 2 cut(s) 782, 1581
BbsI GAAGAC 3 cut(s) 465, 1371, 1539
Bbv12I GWGCWC 1 cut(s) 1582
BbvI GCAGC 1 cut(s) 1256
BccI CCATC 3 cut(s) 454, 1126, 1177
BcgI CGANNNNNNTGC 2 cut(s) 1550, 1584
BciT130I CCWGG 1 cut(s) 613
BcoDI GTCTC 9 cut(s) 585, 707, 850, 909, 1110, 1214, 1323, 1414, 1419
BfaI CTAG 4 cut(s) 258, 453, 878, 1388
BfmI CTRYAG 3 cut(s) 271, 708, 1229
BfrI CTTAAG 1 cut(s) 1265
BglII AGATCT 1 cut(s) 1138
BisI GCNGC 2 cut(s) 851, 1245
BlsI GCNGC 2 cut(s) 852, 1246
Bme1390I CCNGG 1 cut(s) 613
BmeRI GACNNNNNGTC 1 cut(s) 1150
BmeT110I CYCGRG 1 cut(s) 588
BmgT120I GGNCC 2 cut(s) 993, 1174
BmiI GGNNCC 1 cut(s) 441
BmrFI CCNGG 1 cut(s) 613
BmsI GCATC 6 cut(s) 151, 649, 999, 1231, 1307, 1468
BpiI GAAGAC 3 cut(s) 465, 1371, 1539
BpmI CTGGAG 1 cut(s) 528
Bpu10I CCTNAGC 1 cut(s) 989
Bpu14I TTCGAA 1 cut(s) 967
BpuEI CTTGAG 2 cut(s) 158, 1351
BsaHI GRCGYC 1 cut(s) 1376
BsaJI CCNNGG 1 cut(s) 983
BsaWI WCCGGW 2 cut(s) 569, 1339
BsaXI ACNNNNNCTCC 4 cut(s) 1181, 1211, 1569, 1599
Bsc4I CCNNNNNNNGG 4 cut(s) 329, 1085, 1153, 1183
Bse21I CCTNAGG 1 cut(s) 1178
BseBI CCWGG 1 cut(s) 613
BseDI CCNNGG 1 cut(s) 983
BseGI GGATG 7 cut(s) 18, 142, 469, 1188, 1246, 1277, 1298
BseLI CCNNNNNNNGG 4 cut(s) 329, 1085, 1153, 1183
BseMII CTCAG 4 cut(s) 54, 348, 615, 1169
BseRI GAGGAG 4 cut(s) 531, 1108, 1202, 1553
BseXI GCAGC 1 cut(s) 1256
BshFI GGCC 3 cut(s) 995, 1059, 1176
BsiHKAI GWGCWC 1 cut(s) 1582
BsiHKCI CYCGRG 1 cut(s) 588
BsiSI CCGG 2 cut(s) 570, 1340
BslFI GGGAC 1 cut(s) 663
BslI CCNNNNNNNGG 4 cut(s) 329, 1085, 1153, 1183
BsmAI GTCTC 9 cut(s) 585, 707, 850, 909, 1110, 1214, 1323, 1414, 1419
BsmBI CGTCTC 2 cut(s) 585, 1110
BsmFI GGGAC 1 cut(s) 663
BsmI GAATGC 2 cut(s) 514, 975
BsnI GGCC 3 cut(s) 995, 1059, 1176
BsoBI CYCGRG 1 cut(s) 588
Bsp119I TTCGAA 1 cut(s) 967
Bsp1286I GDGCHC 1 cut(s) 1582
Bsp143I GATC 4 cut(s) 90, 382, 1138, 1554
BspACI CCGC 5 cut(s) 803, 824, 851, 975, 1247
BspANI GGCC 3 cut(s) 995, 1059, 1176
BspCNI CTCAG 4 cut(s) 55, 349, 616, 1170
BspHI TCATGA 1 cut(s) 520
BspLI GGNNCC 1 cut(s) 441
BspQI GCTCTTC 2 cut(s) 555, 1608
BspT104I TTCGAA 1 cut(s) 967
BspTI CTTAAG 1 cut(s) 1265
BssECI CCNNGG 1 cut(s) 983
BssMI GATC 4 cut(s) 90, 382, 1138, 1554
BssNI GRCGYC 1 cut(s) 1376
BssSI CACGAG 2 cut(s) 782, 1581
Bst2BI CACGAG 2 cut(s) 782, 1581
Bst2UI CCWGG 1 cut(s) 613
Bst4CI ACNGT 2 cut(s) 685, 1148
Bst6I CTCTTC 3 cut(s) 555, 1417, 1608
BstACI GRCGYC 1 cut(s) 1376
BstAFI CTTAAG 1 cut(s) 1265
BstBI TTCGAA 1 cut(s) 967
BstC8I GCNNGC 3 cut(s) 975, 1483, 1569
BstF5I GGATG 7 cut(s) 18, 142, 469, 1188, 1246, 1277, 1298
BstKTI GATC 4 cut(s) 93, 385, 1141, 1557
BstMAI GTCTC 9 cut(s) 585, 707, 850, 909, 1110, 1214, 1323, 1414, 1419
BstMBI GATC 4 cut(s) 90, 382, 1138, 1554
BstMWI GCNNNNNNNGC 2 cut(s) 800, 1577
BstNI CCWGG 1 cut(s) 613
BstSCI CCNGG 1 cut(s) 611
BstSFI CTRYAG 3 cut(s) 271, 708, 1229
BstV1I GCAGC 1 cut(s) 1256
BstV2I GAAGAC 3 cut(s) 465, 1371, 1539
BstX2I RGATCY 1 cut(s) 1138
BstXI CCANNNNNNTGG 1 cut(s) 1309
BstYI RGATCY 1 cut(s) 1138
Bsu36I CCTNAGG 1 cut(s) 1178
BsuRI GGCC 3 cut(s) 995, 1059, 1176
BtsCI GGATG 7 cut(s) 18, 142, 469, 1188, 1246, 1277, 1298
Cac8I GCNNGC 3 cut(s) 975, 1483, 1569
CaiI CAGNNNCTG 1 cut(s) 761
CciI TCATGA 1 cut(s) 520
Cfr13I GGNCC 2 cut(s) 993, 1174
CseI GACGC 2 cut(s) 602, 1365
Csp6I GTAC 3 cut(s) 628, 1064, 1461
CviAII CATG 6 cut(s) 154, 293, 421, 521, 1458, 1475
CviQI GTAC 3 cut(s) 628, 1064, 1461
DpnI GATC 4 cut(s) 92, 384, 1140, 1556
DpnII GATC 4 cut(s) 90, 382, 1138, 1554
DriI GACNNNNNGTC 1 cut(s) 1150
Eam1104I CTCTTC 3 cut(s) 555, 1417, 1608
Eam1105I GACNNNNNGTC 1 cut(s) 1150
EarI CTCTTC 3 cut(s) 555, 1417, 1608
Eco32I GATATC 1 cut(s) 1020
Eco57I CTGAAG 2 cut(s) 1557, 1593
Eco81I CCTNAGG 1 cut(s) 1178
Eco88I CYCGRG 1 cut(s) 588
EcoO109I RGGNCCY 1 cut(s) 993
EcoRII CCWGG 1 cut(s) 611
EcoRV GATATC 1 cut(s) 1020
Esp3I CGTCTC 2 cut(s) 585, 1110
FaeI CATG 6 cut(s) 157, 296, 424, 524, 1461, 1478
FalI AAGNNNNNCTT 2 cut(s) 1555, 1587
FaqI GGGAC 1 cut(s) 663
FatI CATG 6 cut(s) 153, 292, 420, 520, 1457, 1474
FauI CCCGC 2 cut(s) 796, 982
Fnu4HI GCNGC 2 cut(s) 851, 1245
FokI GGATG 7 cut(s) 25, 129, 476, 1195, 1253, 1264, 1285
Fsp4HI GCNGC 2 cut(s) 851, 1245
FspBI CTAG 4 cut(s) 258, 453, 878, 1388
GluI GCNGC 2 cut(s) 851, 1245
GsuI CTGGAG 1 cut(s) 528
HaeIII GGCC 3 cut(s) 995, 1059, 1176
HapII CCGG 2 cut(s) 570, 1340
HgaI GACGC 2 cut(s) 602, 1365
Hin1I GRCGYC 1 cut(s) 1376
Hin1II CATG 6 cut(s) 157, 296, 424, 524, 1461, 1478
HincII GTYRAC 1 cut(s) 134
HindII GTYRAC 1 cut(s) 134
HpaII CCGG 2 cut(s) 570, 1340
HphI GGTGA 2 cut(s) 63, 1301
Hpy166II GTNNAC 1 cut(s) 134
Hpy8I GTNNAC 1 cut(s) 134
Hpy99I CGWCG 2 cut(s) 1126, 1562
HpyAV CCTTC 3 cut(s) 802, 1231, 1617
HpyCH4III ACNGT 2 cut(s) 685, 1148
HpyCH4V TGCA 3 cut(s) 1012, 1244, 1485
HpyF10VI GCNNNNNNNGC 2 cut(s) 800, 1577
Hsp92I GRCGYC 1 cut(s) 1376
Hsp92II CATG 6 cut(s) 157, 296, 424, 524, 1461, 1478
Kzo9I GATC 4 cut(s) 90, 382, 1138, 1554
LguI GCTCTTC 2 cut(s) 555, 1608
LmnI GCTCC 3 cut(s) 439, 1209, 1577
Lsp1109I GCAGC 1 cut(s) 1256
LweI GCATC 6 cut(s) 151, 649, 999, 1231, 1307, 1468
MaeI CTAG 4 cut(s) 258, 453, 878, 1388
MaeIII GTNAC 2 cut(s) 1253, 1289
MalI GATC 4 cut(s) 92, 384, 1140, 1556
MboI GATC 4 cut(s) 90, 382, 1138, 1554
MfeI CAATTG 1 cut(s) 396
MflI RGATCY 1 cut(s) 1138
MhlI GDGCHC 1 cut(s) 1582
MluCI AATT 6 cut(s) 105, 376, 396, 403, 1051, 1082
MlyI GAGTC 7 cut(s) 46, 645, 911, 1174, 1325, 1421, 1511
MmeI TCCRAC 2 cut(s) 1134, 1326
MroXI GAANNNNTTC 2 cut(s) 238, 963
MseI TTAA 5 cut(s) 213, 348, 1266, 1508, 1621
MslI CAYNNNNRTG 3 cut(s) 158, 519, 525
MspA1I CMGCKG 1 cut(s) 1247
MspCI CTTAAG 1 cut(s) 1265
MspI CCGG 2 cut(s) 570, 1340
MspR9I CCNGG 1 cut(s) 613
MunI CAATTG 1 cut(s) 396
Mva1269I GAATGC 2 cut(s) 514, 975
MvaI CCWGG 1 cut(s) 613
MwoI GCNNNNNNNGC 2 cut(s) 800, 1577
NdeII GATC 4 cut(s) 90, 382, 1138, 1554
NlaIII CATG 6 cut(s) 157, 296, 424, 524, 1461, 1478
NlaIV GGNNCC 1 cut(s) 441
NmuCI GTSAC 1 cut(s) 1289
NspV TTCGAA 1 cut(s) 967
PaeR7I CTCGAG 1 cut(s) 588
PagI TCATGA 1 cut(s) 520
PciSI GCTCTTC 2 cut(s) 555, 1608
PcsI WCGNNNNNNNCGW 2 cut(s) 1100, 1160
PctI GAATGC 2 cut(s) 514, 975
PdmI GAANNNNTTC 2 cut(s) 238, 963
PfeI GAWTC 8 cut(s) 127, 371, 467, 584, 946, 1520, 1590, 1595
PflMI CCANNNNNTGG 1 cut(s) 329
PkrI GCNGC 2 cut(s) 852, 1246
PleI GAGTC 7 cut(s) 45, 645, 910, 1173, 1325, 1421, 1510
PpsI GAGTC 7 cut(s) 45, 645, 910, 1173, 1325, 1421, 1510
Psp6I CCWGG 1 cut(s) 611
PspGI CCWGG 1 cut(s) 611
PspN4I GGNNCC 1 cut(s) 441
PspPI GGNCC 2 cut(s) 993, 1174
PstNI CAGNNNCTG 1 cut(s) 761
PsuI RGATCY 1 cut(s) 1138
RsaI GTAC 3 cut(s) 629, 1065, 1462
RsaNI GTAC 3 cut(s) 628, 1064, 1461
RseI CAYNNNNRTG 3 cut(s) 158, 519, 525
SapI GCTCTTC 2 cut(s) 555, 1608
SaqAI TTAA 5 cut(s) 213, 348, 1266, 1508, 1621
SatI GCNGC 2 cut(s) 851, 1245
Sau3AI GATC 4 cut(s) 90, 382, 1138, 1554
Sau96I GGNCC 2 cut(s) 993, 1174
SchI GAGTC 7 cut(s) 46, 645, 911, 1174, 1325, 1421, 1511
ScrFI CCNGG 1 cut(s) 613
SduI GDGCHC 1 cut(s) 1582
SfaNI GCATC 6 cut(s) 151, 649, 999, 1231, 1307, 1468
SfcI CTRYAG 3 cut(s) 271, 708, 1229
Sfr274I CTCGAG 1 cut(s) 588
SfuI TTCGAA 1 cut(s) 967
SlaI CTCGAG 1 cut(s) 588
SmiMI CAYNNNNRTG 3 cut(s) 158, 519, 525
SmlI CTYRAG 4 cut(s) 137, 588, 1265, 1366
SmoI CTYRAG 4 cut(s) 137, 588, 1265, 1366
Sse9I AATT 6 cut(s) 105, 376, 396, 403, 1051, 1082
SsiI CCGC 5 cut(s) 803, 824, 851, 975, 1247
SspMI CTAG 4 cut(s) 258, 453, 878, 1388
StyD4I CCNGG 1 cut(s) 611
TaaI ACNGT 2 cut(s) 685, 1148
TaqI TCGA 8 cut(s) 482, 589, 967, 998, 1094, 1557, 1593, 1598
TasI AATT 6 cut(s) 105, 376, 396, 403, 1051, 1082
TatI WGTACW 1 cut(s) 627
TauI GCSGC 1 cut(s) 853
TfiI GAWTC 8 cut(s) 127, 371, 467, 584, 946, 1520, 1590, 1595
Tru1I TTAA 5 cut(s) 213, 348, 1266, 1508, 1621
Tru9I TTAA 5 cut(s) 213, 348, 1266, 1508, 1621
TseFI GTSAC 1 cut(s) 1289
TseI GCWGC 1 cut(s) 1244
Tsp45I GTSAC 1 cut(s) 1289
TspGWI ACGGA 1 cut(s) 1092
Van91I CCANNNNNTGG 1 cut(s) 329
Vha464I CTTAAG 1 cut(s) 1265
XapI RAATTY 2 cut(s) 403, 1082
XbaI TCTAGA 1 cut(s) 452
XhoI CTCGAG 1 cut(s) 588
XmnI GAANNNNTTC 2 cut(s) 238, 963
XspI CTAG 4 cut(s) 258, 453, 878, 1388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.