Rroxscaffold_1G00023350

Protein UPSTREAM OF FLC isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006878
Physical Location & Seq
Forward (+)
28818807 .. 28823050
4244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00023350.1

Sequence Viewer

Length: 1647 bp
ATGGAGGGTAGGATGAAAAAGTATAGACAAGTGAGTCCTGAGAGGGCTATAGTGTGGACTGAGAAGTCGCCGAAATACCACCAGAATCGGAAAGTTCCGGTGGTTTATTACCTCTGCCGGAACCGACAGCTTGAACACCCTCATTTCATGGAGGTTCCTGTAAGCTCTTCTCAAGGTCTCTACCTCAGAGATGTGATTAATAAGCTTAATGCTCTGAGGGGAAGAGGGATAGCATCCATGTATTCATGGTCTTCTAAGAGAAGCTATAAGAATGGGTATGTTTGGCATGATCTCAGTGAAGATGATGTGATTCTTCCAGCCCATGGAGACGAGTACGTCCTCAAAGGTTCTGAGATCTTTGATGAATCCAATTCAGATCGATTCAGTCCGATAGCAAATGTCAAAATCCAGAACTTGAAACAATTACCGGAACCAACATCTTCTAGAAACCAAGATGATTCTTCATCGTCTTCCAGTTTGAATGGAAAGGAGACAAAGCAGTCCCATGAAGATGAGCTCTCTCCTCCAGTTGAACGACCAGGAGGCTCTTCCAGTGTGTCTTCAGAGTCTGGAAAGAATTCTTCTTGGGGTGGTTCTCTGAGCTTGACAGAGTACAAGGTTTACAAGAGTGATGGTTTAGCTGATGCTTCTACACAGACTGAAGAAAATGTGAACAAACTTAAAGCTCTAGAAACATGTACAAGGGGTGTTTCCACAGATGATGGGTCACTAGAACCTGATTGCAGTTATTACCAAGACCGAATTCCATGTTTGAAAGAGAATTCTGAGATTTGCAGTGATTCTGTTTCCCCTCCTCCATCTTCCTCAAGTGCATCTTCATCGGGTGGGAAGACTGAAACTTTAGAATCACTTATTAGGGCTGATGTTAGTAAAATTAACAGTTTTAGAATTCTTGAAGAGGAAGAGCTTCGAATGCCAAACAATGCAAGGCTCAAGGCTAGTAATGTACTTATGCAACTCATTTCATGTGGGTCAATATCAGTGAAGGATCATAGTTTTGGTCTTGTTCCAACCTACAAGCCCAGGTTTTCCCATTCTAAGTTTCCCTCGCCGTTATTTTCTAGTTCAATAATGCTGGGTGAGCTAGACAGCCTGTCAGAGAATCCAAGTCTAAAGGGTTTGAGATTGGAAGATAAAGAATATTTCAGTGGGAGCTTGATTGATGGTAAAGTGCTCAAGGATGAAGATCGACATACAGCTCTAAAGCGATCGTCTTCATACAATGCCGACAGTAAGGGAGAGTTGAGACATCTGTTGGACACCAAAGAGTATTGCAAGTTGAATAGGGTGACTTCAGAGCTTGGGGGTTATAGGAGTTGTAATAAGACGCCAGATTCAGTTGAGGTCAAAGAGAAGGAGTCTACCTCAGGACGCTCAAAATGCATCCTAAGATCGATCAAGGCTTCACTAAACAAGCAACCACGGAGTGAATCTATGAGATCTCCTATTTCTGATAAACCAAGGAACTCCTCAGATGGAGTTGATGGTGTACAAAGCAATACCCCTACTGTATCCAATGGAGGTGGTAAAAGAATCACAGATCCATCCTCTGTTAAAAAGCAATCGAAAAGGGTAGATGCTTTCAGAGGAGAGAAGGAGAAGGTTATCAAAATCGAAGAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

548

Amino Acids

60.83

Weight (kDa)

8.49

Isoelectric Point (pI)

58.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 32 - 120 8.1e-50 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 4 cut(s) 33, 64, 335, 499
AccB7I CCANNNNNTGG 1 cut(s) 323
AccI GTMKAC 1 cut(s) 1382
AclWI GGATC 2 cut(s) 1017, 1556
AcsI RAATTY 4 cut(s) 577, 762, 781, 909
AcuI CTGAAG 3 cut(s) 546, 681, 1299
AcyI GRCGYC 1 cut(s) 1349
AdeI CACNNNGTG 1 cut(s) 1448
AfaI GTAC 5 cut(s) 335, 614, 700, 969, 1512
AfiI CCNNNNNNNGG 1 cut(s) 323
AflIII ACRYGT 1 cut(s) 695
AgsI TTSAA 8 cut(s) 134, 418, 481, 533, 775, 917, 1089, 1303
AhdI GACNNNNNGTC 1 cut(s) 1114
AjnI CCWGG 2 cut(s) 538, 1043
AjuI GAANNNNNNNTTGG 2 cut(s) 747, 779
Alw21I GWGCWC 2 cut(s) 519, 1197
Alw26I GTCTC 4 cut(s) 182, 321, 485, 1261
AlwI GGATC 2 cut(s) 1017, 1556
AlwNI CAGNNNCTG 1 cut(s) 569
ApoI RAATTY 4 cut(s) 577, 762, 781, 909
AseI ATTAAT 1 cut(s) 198
Asp700I GAANNNNTTC 2 cut(s) 577, 927
AsuHPI GGTGA 2 cut(s) 1112, 1321
AsuII TTCGAA 1 cut(s) 931
AxyI CCTNAGG 1 cut(s) 1387
BanII GRGCYC 1 cut(s) 519
BbsI GAAGAC 5 cut(s) 243, 462, 552, 857, 1227
Bbv12I GWGCWC 2 cut(s) 519, 1197
BccI CCATC 7 cut(s) 626, 716, 826, 1178, 1490, 1499, 1573
BceAI ACGGC 1 cut(s) 1057
BcgI CGANNNNNNTGC 2 cut(s) 822, 856
BciT130I CCWGG 2 cut(s) 540, 1045
BciVI GTATCC 1 cut(s) 1543
BcoDI GTCTC 4 cut(s) 182, 321, 485, 1261
BfaI CTAG 6 cut(s) 444, 689, 731, 960, 1083, 1106
BfmI CTRYAG 1 cut(s) 48
BfuI GTATCC 1 cut(s) 1543
BglII AGATCT 2 cut(s) 354, 1460
Bme1390I CCNGG 2 cut(s) 540, 1045
BmeRI GACNNNNNGTC 1 cut(s) 1114
BmiI GGNNCC 3 cut(s) 122, 156, 432
BmrFI CCNGG 2 cut(s) 540, 1045
BmsI GCATC 5 cut(s) 242, 634, 842, 1413, 1588
BpiI GAAGAC 5 cut(s) 243, 462, 552, 857, 1227
BplI GAGNNNNNCTC 2 cut(s) 1370, 1402
BpmI CTGGAG 1 cut(s) 510
Bpu14I TTCGAA 1 cut(s) 931
BpuEI CTTGAG 4 cut(s) 156, 811, 938, 1181
Bsa29I ATCGAT 2 cut(s) 379, 1415
BsaBI GATNNNNATC 1 cut(s) 1206
BsaHI GRCGYC 1 cut(s) 1349
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 4 cut(s) 322, 1043, 1442, 1481
BsaWI WCCGGW 2 cut(s) 97, 427
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse1I ACTGG 3 cut(s) 474, 527, 552
Bse21I CCTNAGG 1 cut(s) 1387
Bse8I GATNNNNATC 1 cut(s) 1206
BseBI CCWGG 2 cut(s) 540, 1045
BseCI ATCGAT 2 cut(s) 379, 1415
BseDI CCNNGG 4 cut(s) 322, 1043, 1442, 1481
BseGI GGATG 5 cut(s) 18, 233, 1207, 1404, 1565
BseJI GATNNNNATC 1 cut(s) 1206
BseLI CCNNNNNNNGG 1 cut(s) 323
BseNI ACTGG 3 cut(s) 474, 527, 552
BseRI GAGGAG 4 cut(s) 513, 804, 1480, 1623
BseYI CCCAGC 1 cut(s) 1096
Bsh1285I CGRYCG 1 cut(s) 1232
BshVI ATCGAT 2 cut(s) 379, 1415
BsiEI CGRYCG 1 cut(s) 1232
BsiHKAI GWGCWC 2 cut(s) 519, 1197
BsiSI CCGG 3 cut(s) 98, 118, 428
BslFI GGGAC 1 cut(s) 487
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 4 cut(s) 182, 321, 485, 1261
BsmBI CGTCTC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 487
BsmI GAATGC 1 cut(s) 939
Bso31I GGTCTC 1 cut(s) 182
Bsp119I TTCGAA 1 cut(s) 931
Bsp1286I GDGCHC 2 cut(s) 519, 1197
Bsp1407I TGTACA 2 cut(s) 698, 1510
Bsp19I CCATGG 1 cut(s) 322
BspDI ATCGAT 2 cut(s) 379, 1415
BspLI GGNNCC 3 cut(s) 122, 156, 432
BspPI GGATC 2 cut(s) 1017, 1556
BspQI GCTCTTC 3 cut(s) 172, 553, 918
BspT104I TTCGAA 1 cut(s) 931
BspTNI GGTCTC 1 cut(s) 182
BsrGI TGTACA 2 cut(s) 698, 1510
BsrI ACTGG 3 cut(s) 474, 527, 552
BssECI CCNNGG 4 cut(s) 322, 1043, 1442, 1481
BssNI GRCGYC 1 cut(s) 1349
BssT1I CCWWGG 2 cut(s) 322, 1481
Bst2UI CCWGG 2 cut(s) 540, 1045
Bst4CI ACNGT 3 cut(s) 902, 1253, 1531
Bst6I CTCTTC 5 cut(s) 172, 217, 553, 912, 918
BstACI GRCGYC 1 cut(s) 1349
BstAUI TGTACA 2 cut(s) 698, 1510
BstBI TTCGAA 1 cut(s) 931
BstDSI CCRYGG 2 cut(s) 322, 1442
BstF5I GGATG 5 cut(s) 18, 233, 1207, 1404, 1565
BstMAI GTCTC 4 cut(s) 182, 321, 485, 1261
BstMCI CGRYCG 1 cut(s) 1232
BstMWI GCNNNNNNNGC 3 cut(s) 934, 1102, 1401
BstNI CCWGG 2 cut(s) 540, 1045
BstNSI RCATGY 1 cut(s) 699
BstSCI CCNGG 2 cut(s) 538, 1043
BstSFI CTRYAG 1 cut(s) 48
BstV2I GAAGAC 5 cut(s) 243, 462, 552, 857, 1227
BstX2I RGATCY 3 cut(s) 354, 1460, 1561
BstYI RGATCY 3 cut(s) 354, 1460, 1561
Bsu15I ATCGAT 2 cut(s) 379, 1415
Bsu36I CCTNAGG 1 cut(s) 1387
BsuI GTATCC 1 cut(s) 1543
BsuTUI ATCGAT 2 cut(s) 379, 1415
BtgI CCRYGG 2 cut(s) 322, 1442
BtsCI GGATG 5 cut(s) 18, 233, 1207, 1404, 1565
BtsI GCAGTG 1 cut(s) 802
BtsIMutI CAGTG 5 cut(s) 301, 559, 802, 1008, 1174
CaiI CAGNNNCTG 1 cut(s) 569
ClaI ATCGAT 2 cut(s) 379, 1415
CseI GACGC 2 cut(s) 1357, 1401
Csp6I GTAC 5 cut(s) 334, 613, 699, 968, 1511
CspCI CAANNNNNGTGG 2 cut(s) 1525, 1560
CviAII CATG 9 cut(s) 148, 238, 246, 287, 323, 506, 696, 768, 987
CviQI GTAC 5 cut(s) 334, 613, 699, 968, 1511
DraIII CACNNNGTG 1 cut(s) 1448
DrdI GACNNNNNNGTC 4 cut(s) 33, 64, 335, 499
DriI GACNNNNNGTC 1 cut(s) 1114
DseDI GACNNNNNNGTC 4 cut(s) 33, 64, 335, 499
Eam1104I CTCTTC 5 cut(s) 172, 217, 553, 912, 918
Eam1105I GACNNNNNGTC 1 cut(s) 1114
EarI CTCTTC 5 cut(s) 172, 217, 553, 912, 918
Ecl136II GAGCTC 1 cut(s) 517
Eco130I CCWWGG 2 cut(s) 322, 1481
Eco24I GRGCYC 1 cut(s) 519
Eco31I GGTCTC 1 cut(s) 182
Eco53kI GAGCTC 1 cut(s) 517
Eco57I CTGAAG 3 cut(s) 546, 681, 1299
Eco81I CCTNAGG 1 cut(s) 1387
EcoICRI GAGCTC 1 cut(s) 517
EcoRI GAATTC 4 cut(s) 577, 762, 781, 909
EcoRII CCWGG 2 cut(s) 538, 1043
EcoT14I CCWWGG 2 cut(s) 322, 1481
EcoT22I ATGCAT 1 cut(s) 1406
EcoT38I GRGCYC 1 cut(s) 519
ErhI CCWWGG 2 cut(s) 322, 1481
Esp3I CGTCTC 1 cut(s) 321
FaeI CATG 9 cut(s) 151, 241, 249, 290, 326, 509, 699, 771, 990
FalI AAGNNNNNCTT 2 cut(s) 820, 852
FaqI GGGAC 1 cut(s) 487
FatI CATG 9 cut(s) 147, 237, 245, 286, 322, 505, 695, 767, 986
FblI GTMKAC 1 cut(s) 1382
FokI GGATG 5 cut(s) 25, 220, 1214, 1391, 1552
FriOI GRGCYC 1 cut(s) 519
FspBI CTAG 6 cut(s) 444, 689, 731, 960, 1083, 1106
GsaI CCCAGC 1 cut(s) 1100
GsuI CTGGAG 1 cut(s) 510
HapII CCGG 3 cut(s) 98, 118, 428
HgaI GACGC 2 cut(s) 1357, 1401
Hin1I GRCGYC 1 cut(s) 1349
Hin1II CATG 9 cut(s) 151, 241, 249, 290, 326, 509, 699, 771, 990
HindIII AAGCTT 1 cut(s) 203
HpaII CCGG 3 cut(s) 98, 118, 428
HphI GGTGA 2 cut(s) 1112, 1321
Hpy166II GTNNAC 5 cut(s) 57, 622, 673, 1383, 1511
Hpy188III TCNNGA 7 cut(s) 38, 409, 444, 570, 689, 914, 1389
Hpy8I GTNNAC 5 cut(s) 57, 622, 673, 1383, 1511
HpyAV CCTTC 4 cut(s) 1000, 1369, 1609, 1615
HpyCH4III ACNGT 3 cut(s) 902, 1253, 1531
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 7 cut(s) 744, 795, 833, 947, 976, 1296, 1404
HpyF10VI GCNNNNNNNGC 3 cut(s) 934, 1102, 1401
HpySE526I ACGT 1 cut(s) 336
Hsp92I GRCGYC 1 cut(s) 1349
Hsp92II CATG 9 cut(s) 151, 241, 249, 290, 326, 509, 699, 771, 990
LguI GCTCTTC 3 cut(s) 172, 553, 918
LmnI GCTCC 1 cut(s) 1173
LweI GCATC 5 cut(s) 242, 634, 842, 1413, 1588
MaeI CTAG 6 cut(s) 444, 689, 731, 960, 1083, 1106
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 2 cut(s) 726, 1309
MflI RGATCY 3 cut(s) 354, 1460, 1561
MhlI GDGCHC 2 cut(s) 519, 1197
MluCI AATT 7 cut(s) 370, 422, 577, 762, 781, 894, 909
MlyI GAGTC 3 cut(s) 43, 575, 1388
MmeI TCCRAC 2 cut(s) 1055, 1257
Mph1103I ATGCAT 1 cut(s) 1406
MroXI GAANNNNTTC 2 cut(s) 577, 927
MseI TTAA 5 cut(s) 198, 207, 681, 897, 1575
MslI CAYNNNNRTG 1 cut(s) 510
MspI CCGG 3 cut(s) 98, 118, 428
MspR9I CCNGG 2 cut(s) 540, 1045
Mva1269I GAATGC 1 cut(s) 939
MvaI CCWGG 2 cut(s) 540, 1045
MwoI GCNNNNNNNGC 3 cut(s) 934, 1102, 1401
NcoI CCATGG 1 cut(s) 322
NlaIII CATG 9 cut(s) 151, 241, 249, 290, 326, 509, 699, 771, 990
NlaIV GGNNCC 3 cut(s) 122, 156, 432
NmuCI GTSAC 2 cut(s) 726, 1309
NsiI ATGCAT 1 cut(s) 1406
NspI RCATGY 1 cut(s) 699
NspV TTCGAA 1 cut(s) 931
PciI ACATGT 1 cut(s) 695
PciSI GCTCTTC 3 cut(s) 172, 553, 918
PctI GAATGC 1 cut(s) 939
PdmI GAANNNNTTC 2 cut(s) 577, 927
PflMI CCANNNNNTGG 1 cut(s) 323
Ple19I CGATCG 1 cut(s) 1232
PleI GAGTC 3 cut(s) 42, 574, 1387
PpsI GAGTC 3 cut(s) 42, 574, 1387
PscI ACATGT 1 cut(s) 695
PshBI ATTAAT 1 cut(s) 198
Psp124BI GAGCTC 1 cut(s) 519
Psp6I CCWGG 2 cut(s) 538, 1043
PspFI CCCAGC 1 cut(s) 1096
PspGI CCWGG 2 cut(s) 538, 1043
PspN4I GGNNCC 3 cut(s) 122, 156, 432
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 3 cut(s) 354, 1460, 1561
PvuI CGATCG 1 cut(s) 1232
RsaI GTAC 5 cut(s) 335, 614, 700, 969, 1512
RsaNI GTAC 5 cut(s) 334, 613, 699, 968, 1511
RseI CAYNNNNRTG 1 cut(s) 510
SacI GAGCTC 1 cut(s) 519
SapI GCTCTTC 3 cut(s) 172, 553, 918
SaqAI TTAA 5 cut(s) 198, 207, 681, 897, 1575
SchI GAGTC 3 cut(s) 43, 575, 1388
ScrFI CCNGG 2 cut(s) 540, 1045
SduI GDGCHC 2 cut(s) 519, 1197
SfaNI GCATC 5 cut(s) 242, 634, 842, 1413, 1588
SfcI CTRYAG 1 cut(s) 48
SfuI TTCGAA 1 cut(s) 931
SmiMI CAYNNNNRTG 1 cut(s) 510
SmlI CTYRAG 4 cut(s) 171, 826, 953, 1196
SmoI CTYRAG 4 cut(s) 171, 826, 953, 1196
Sse9I AATT 7 cut(s) 370, 422, 577, 762, 781, 894, 909
SspI AATATT 1 cut(s) 1163
SspMI CTAG 6 cut(s) 444, 689, 731, 960, 1083, 1106
SstI GAGCTC 1 cut(s) 519
StyD4I CCNGG 2 cut(s) 538, 1043
StyI CCWWGG 2 cut(s) 322, 1481
TaaI ACNGT 3 cut(s) 902, 1253, 1531
TaiI ACGT 1 cut(s) 339
TaqI TCGA 6 cut(s) 379, 931, 1210, 1415, 1586, 1635
TaqII GACCGA 1 cut(s) 774
TasI AATT 7 cut(s) 370, 422, 577, 762, 781, 894, 909
TatI WGTACW 4 cut(s) 612, 698, 967, 1510
Tru1I TTAA 5 cut(s) 198, 207, 681, 897, 1575
Tru9I TTAA 5 cut(s) 198, 207, 681, 897, 1575
TscAI CASTG 5 cut(s) 301, 559, 802, 1008, 1174
TseFI GTSAC 2 cut(s) 726, 1309
Tsp45I GTSAC 2 cut(s) 726, 1309
TspGWI ACGGA 1 cut(s) 1459
TspRI CASTG 5 cut(s) 301, 559, 802, 1008, 1174
Van91I CCANNNNNTGG 1 cut(s) 323
VspI ATTAAT 1 cut(s) 198
XapI RAATTY 4 cut(s) 577, 762, 781, 909
XbaI TCTAGA 2 cut(s) 443, 688
XceI RCATGY 1 cut(s) 699
XmiI GTMKAC 1 cut(s) 1382
XmnI GAANNNNTTC 2 cut(s) 577, 927
XspI CTAG 6 cut(s) 444, 689, 731, 960, 1083, 1106
Zsp2I ATGCAT 1 cut(s) 1406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.