Rw5G035140

Protein UPSTREAM OF FLC isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006878
Physical Location & Seq
Reverse (-)
60682391 .. 60685178
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G035140.1

Sequence Viewer

Length: 1638 bp
ATGGAGGGTAGGATGAAAAAGTATAGACAAGTGAGTCCTGAGAGGGCTAAAGTGTGGACCGAGAAGTCGCCGAAATACCACCAGAATCGGAAAGTTCCGGTGGTTTATTACCTCTGCCGGAACCGACAGCTTGAACACCCTCATTTCATGGAGGTTACTGTAAGCTCTTCTCAAGGTCTCTACCTCAGAGATGTGATTAATAAGCTTAATGCTCTGAGGGGCAGAGGGATAGCATCCATGTATTCATGGTCTTCTAAGAGAAGCTATAAGAATGGGTATGTTTGGCATGATCTCAGTGAAGATGATGTGATTCTTCCAGCCCATGGAGACGAGTACGTCCTCAAAGGTTCTGAGATCTTTGATGAATCCAATTCAGATCGATTCAGTCCGATAGCAAATGTCAAAATCCAGAACTTGAAACAATTACCGGAACCAACATCTTCTAGAAGCCAAGGTGATTCTTCATCGTCTTCCAGTTTGAATGGAAAGGAGACAAAGCAGTCCCATGAAGATGAGATCTCTCCTCCAGTTGAACGACCAGGAGGCTCTTCCAGTGTGTCTTCAGAGTCTGGAAAGAATTCTTCTTGGGGTGGTTCTCTGAGCTTGACAGAGTACAAGGTTTACAAGAGTGATGGTTTAGCTGATGCTTCTACACAGACTGAAGAAAATGTGAACAAACTTAAAGCTCTAGAAACATGTACAAGGGGTGTTTCCACAGATGATGGGTCACTAGAACCTGATTGCAGTTATTACCAAGACCGAATTCCATGTTTGAAAGAGAATTCTGAGATTTGCAGTGATTCTGTTTCCCCTCCTCCATCTTCCTCAAGTGCATCTTCATCAGGTGGGAAGACTGAAACTTTAGAATCACTTATTAGGGCTGATGTTAGTAAAATTAACAGTTTTAGAATTCTTGAAGAGGAAGAGCTTCGAATGCCAAACAATGCAAGGCTCAAGGCTAGTAATGTACTTATGCAACTCATTTCATGTGGGTCAATATCAGTGAAGGATCATAGTTTTGGTCTTGTTCCAACCTACAAGCCCAGGTTTTCCCATTCTAAGTTTCCCTCACCATTATTTTCTAGTTCAATAATGCTGGGTGAGCTAGACAGCCTGTCAGAGAATCCAAGGCTAAAGGGTTTGAGATTGGAAGATAAAGAATATTTCAGTGGGAGCTTGATTGATAGTAAAGTGCTCAAGGAGGAAGATGGACATACAGCTCTAAAGCGATCGTCTTCATACAATGCCGACAGGAGTTGTAATAAGACGCCAGATTCAGTTGAGGTCAAAGAGAAGGAGTCTACCTCAGGACGCTCAAAATGCATCCTAAGATCGATCAAGGCTTCACTAAACAAGCAATCACGGAGTGAATCTATGAGATCTCCTATTTCTGATAAACCAAGGAACTCCTCAGATGGAGTTGATGGTGTAGATGGTGTACAAAGCAATACCCCTACTGTATCCGATGGAGGTAGTAAAAGAATCACAAATCCATCCTCTGTTAAAAAGCAATCAAAAAGGGTAGATGCTTTCAGAGGAGAGAAGGAGAAGGTTATCAAAATCGAAGAAAGAGCTCGGGTTGTAATCCAATCAAGAGCACCTCGTGATGCTGCTGCCGTTGGCAGCTCATGGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF966

Protein Analysis

545

Amino Acids

60.24

Weight (kDa)

8.82

Isoelectric Point (pI)

61.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOK PF06136 32 - 120 8.7e-50 SOSEKI protein DIX-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 4 cut(s) 33, 64, 335, 499
AccB7I CCANNNNNTGG 1 cut(s) 323
AccI GTMKAC 1 cut(s) 1301
AclWI GGATC 1 cut(s) 1017
AcsI RAATTY 4 cut(s) 577, 762, 781, 909
AcuI CTGAAG 2 cut(s) 546, 681
AcyI GRCGYC 1 cut(s) 1268
AdeI CACNNNGTG 2 cut(s) 1367, 1604
AfaI GTAC 5 cut(s) 335, 614, 700, 969, 1440
AfiI CCNNNNNNNGG 1 cut(s) 323
AflIII ACRYGT 1 cut(s) 695
AgsI TTSAA 7 cut(s) 134, 418, 481, 533, 775, 917, 1089
AhdI GACNNNNNGTC 1 cut(s) 1114
AjnI CCWGG 2 cut(s) 538, 1043
AjuI GAANNNNNNNTTGG 2 cut(s) 747, 779
Alw21I GWGCWC 3 cut(s) 1197, 1576, 1600
Alw26I GTCTC 3 cut(s) 182, 321, 485
AlwI GGATC 1 cut(s) 1017
AlwNI CAGNNNCTG 1 cut(s) 569
Ama87I CYCGRG 1 cut(s) 1575
ApeKI GCWGC 3 cut(s) 1610, 1613, 1623
ApoI RAATTY 4 cut(s) 577, 762, 781, 909
AseI ATTAAT 1 cut(s) 198
Asp700I GAANNNNTTC 2 cut(s) 577, 927
AspS9I GGNCC 1 cut(s) 57
AsuHPI GGTGA 3 cut(s) 467, 1062, 1112
AsuII TTCGAA 1 cut(s) 931
AvaI CYCGRG 1 cut(s) 1575
AvaII GGWCC 1 cut(s) 57
AxyI CCTNAGG 1 cut(s) 1306
BanII GRGCYC 1 cut(s) 1576
BauI CACGAG 1 cut(s) 1602
BbsI GAAGAC 5 cut(s) 243, 462, 552, 857, 1227
Bbv12I GWGCWC 3 cut(s) 1197, 1576, 1600
BbvI GCAGC 2 cut(s) 1597, 1600
BccI CCATC 9 cut(s) 626, 716, 826, 1202, 1409, 1418, 1427, 1460, 1501
BceAI ACGGC 1 cut(s) 1601
BciT130I CCWGG 2 cut(s) 540, 1045
BciVI GTATCC 1 cut(s) 1471
BcoDI GTCTC 3 cut(s) 182, 321, 485
BfaI CTAG 6 cut(s) 444, 689, 731, 960, 1083, 1106
BfuI GTATCC 1 cut(s) 1471
BglII AGATCT 3 cut(s) 354, 516, 1379
BisI GCNGC 3 cut(s) 1611, 1614, 1624
BlsI GCNGC 3 cut(s) 1612, 1615, 1625
Bme1390I CCNGG 2 cut(s) 540, 1045
Bme18I GGWCC 1 cut(s) 57
BmeRI GACNNNNNGTC 1 cut(s) 1114
BmeT110I CYCGRG 1 cut(s) 1575
BmgT120I GGNCC 1 cut(s) 57
BmiI GGNNCC 2 cut(s) 122, 432
BmrFI CCNGG 2 cut(s) 540, 1045
BmsI GCATC 6 cut(s) 242, 634, 842, 1332, 1516, 1597
BpiI GAAGAC 5 cut(s) 243, 462, 552, 857, 1227
BplI GAGNNNNNCTC 2 cut(s) 1289, 1321
BpmI CTGGAG 1 cut(s) 510
Bpu14I TTCGAA 1 cut(s) 931
BpuEI CTTGAG 4 cut(s) 156, 811, 938, 1181
Bsa29I ATCGAT 2 cut(s) 379, 1334
BsaHI GRCGYC 1 cut(s) 1268
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 5 cut(s) 322, 451, 1043, 1127, 1400
BsaWI WCCGGW 2 cut(s) 97, 427
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse1I ACTGG 3 cut(s) 474, 527, 552
Bse21I CCTNAGG 1 cut(s) 1306
BseBI CCWGG 2 cut(s) 540, 1045
BseCI ATCGAT 2 cut(s) 379, 1334
BseDI CCNNGG 5 cut(s) 322, 451, 1043, 1127, 1400
BseGI GGATG 4 cut(s) 18, 233, 1323, 1493
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMII CTCAG 9 cut(s) 30, 199, 206, 307, 342, 590, 777, 1320, 1425
BseNI ACTGG 3 cut(s) 474, 527, 552
BseRI GAGGAG 4 cut(s) 513, 804, 1399, 1551
BseXI GCAGC 2 cut(s) 1597, 1600
BseYI CCCAGC 1 cut(s) 1096
Bsh1285I CGRYCG 1 cut(s) 1232
BshVI ATCGAT 2 cut(s) 379, 1334
BsiEI CGRYCG 1 cut(s) 1232
BsiHKAI GWGCWC 3 cut(s) 1197, 1576, 1600
BsiHKCI CYCGRG 1 cut(s) 1575
BsiSI CCGG 3 cut(s) 98, 118, 428
BslFI GGGAC 1 cut(s) 487
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 3 cut(s) 182, 321, 485
BsmBI CGTCTC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 487
BsmI GAATGC 1 cut(s) 939
Bso31I GGTCTC 1 cut(s) 182
BsoBI CYCGRG 1 cut(s) 1575
Bsp119I TTCGAA 1 cut(s) 931
Bsp1286I GDGCHC 3 cut(s) 1197, 1576, 1600
Bsp1407I TGTACA 2 cut(s) 698, 1438
Bsp143I GATC 9 cut(s) 289, 354, 376, 516, 1009, 1229, 1331, 1335, 1379
Bsp19I CCATGG 1 cut(s) 322
BspCNI CTCAG 9 cut(s) 31, 198, 207, 306, 343, 591, 778, 1319, 1424
BspDI ATCGAT 2 cut(s) 379, 1334
BspLI GGNNCC 2 cut(s) 122, 432
BspPI GGATC 1 cut(s) 1017
BspQI GCTCTTC 3 cut(s) 172, 553, 918
BspT104I TTCGAA 1 cut(s) 931
BspTNI GGTCTC 1 cut(s) 182
BsrGI TGTACA 2 cut(s) 698, 1438
BsrI ACTGG 3 cut(s) 474, 527, 552
BssECI CCNNGG 5 cut(s) 322, 451, 1043, 1127, 1400
BssMI GATC 9 cut(s) 289, 354, 376, 516, 1009, 1229, 1331, 1335, 1379
BssNI GRCGYC 1 cut(s) 1268
BssSI CACGAG 1 cut(s) 1602
BssT1I CCWWGG 4 cut(s) 322, 451, 1127, 1400
Bst2BI CACGAG 1 cut(s) 1602
Bst2UI CCWGG 2 cut(s) 540, 1045
Bst4CI ACNGT 3 cut(s) 160, 902, 1459
Bst6I CTCTTC 4 cut(s) 172, 553, 912, 918
BstACI GRCGYC 1 cut(s) 1268
BstAUI TGTACA 2 cut(s) 698, 1438
BstBI TTCGAA 1 cut(s) 931
BstDSI CCRYGG 1 cut(s) 322
BstF5I GGATG 4 cut(s) 18, 233, 1323, 1493
BstKTI GATC 9 cut(s) 292, 357, 379, 519, 1012, 1232, 1334, 1338, 1382
BstMAI GTCTC 3 cut(s) 182, 321, 485
BstMBI GATC 9 cut(s) 289, 354, 376, 516, 1009, 1229, 1331, 1335, 1379
BstMCI CGRYCG 1 cut(s) 1232
BstMWI GCNNNNNNNGC 3 cut(s) 934, 1102, 1320
BstNI CCWGG 2 cut(s) 540, 1045
BstNSI RCATGY 1 cut(s) 699
BstSCI CCNGG 2 cut(s) 538, 1043
BstV1I GCAGC 2 cut(s) 1597, 1600
BstV2I GAAGAC 5 cut(s) 243, 462, 552, 857, 1227
BstX2I RGATCY 3 cut(s) 354, 516, 1379
BstYI RGATCY 3 cut(s) 354, 516, 1379
Bsu15I ATCGAT 2 cut(s) 379, 1334
Bsu36I CCTNAGG 1 cut(s) 1306
BsuI GTATCC 1 cut(s) 1471
BsuTUI ATCGAT 2 cut(s) 379, 1334
BtgI CCRYGG 1 cut(s) 322
BtsCI GGATG 4 cut(s) 18, 233, 1323, 1493
BtsI GCAGTG 1 cut(s) 802
BtsIMutI CAGTG 5 cut(s) 301, 559, 802, 1008, 1174
CaiI CAGNNNCTG 1 cut(s) 569
Cfr13I GGNCC 1 cut(s) 57
ClaI ATCGAT 2 cut(s) 379, 1334
CseI GACGC 2 cut(s) 1276, 1320
Csp6I GTAC 5 cut(s) 334, 613, 699, 968, 1439
CviQI GTAC 5 cut(s) 334, 613, 699, 968, 1439
DpnI GATC 9 cut(s) 291, 356, 378, 518, 1011, 1231, 1333, 1337, 1381
DpnII GATC 9 cut(s) 289, 354, 376, 516, 1009, 1229, 1331, 1335, 1379
DraIII CACNNNGTG 2 cut(s) 1367, 1604
DrdI GACNNNNNNGTC 4 cut(s) 33, 64, 335, 499
DriI GACNNNNNGTC 1 cut(s) 1114
DseDI GACNNNNNNGTC 4 cut(s) 33, 64, 335, 499
Eam1104I CTCTTC 4 cut(s) 172, 553, 912, 918
Eam1105I GACNNNNNGTC 1 cut(s) 1114
EarI CTCTTC 4 cut(s) 172, 553, 912, 918
Ecl136II GAGCTC 1 cut(s) 1574
Eco130I CCWWGG 4 cut(s) 322, 451, 1127, 1400
Eco24I GRGCYC 1 cut(s) 1576
Eco31I GGTCTC 1 cut(s) 182
Eco47I GGWCC 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 1574
Eco57I CTGAAG 2 cut(s) 546, 681
Eco81I CCTNAGG 1 cut(s) 1306
Eco88I CYCGRG 1 cut(s) 1575
EcoICRI GAGCTC 1 cut(s) 1574
EcoRI GAATTC 4 cut(s) 577, 762, 781, 909
EcoRII CCWGG 2 cut(s) 538, 1043
EcoT14I CCWWGG 4 cut(s) 322, 451, 1127, 1400
EcoT22I ATGCAT 1 cut(s) 1325
EcoT38I GRGCYC 1 cut(s) 1576
ErhI CCWWGG 4 cut(s) 322, 451, 1127, 1400
Esp3I CGTCTC 1 cut(s) 321
FalI AAGNNNNNCTT 2 cut(s) 820, 852
FaqI GGGAC 1 cut(s) 487
FblI GTMKAC 1 cut(s) 1301
Fnu4HI GCNGC 3 cut(s) 1611, 1614, 1624
FokI GGATG 4 cut(s) 25, 220, 1310, 1480
FriOI GRGCYC 1 cut(s) 1576
Fsp4HI GCNGC 3 cut(s) 1611, 1614, 1624
FspBI CTAG 6 cut(s) 444, 689, 731, 960, 1083, 1106
GluI GCNGC 3 cut(s) 1611, 1614, 1624
GsaI CCCAGC 1 cut(s) 1100
GsuI CTGGAG 1 cut(s) 510
HapII CCGG 3 cut(s) 98, 118, 428
HgaI GACGC 2 cut(s) 1276, 1320
Hin1I GRCGYC 1 cut(s) 1268
HindIII AAGCTT 1 cut(s) 203
HpaII CCGG 3 cut(s) 98, 118, 428
HphI GGTGA 3 cut(s) 467, 1062, 1112
Hpy166II GTNNAC 5 cut(s) 57, 622, 673, 1302, 1439
Hpy188III TCNNGA 9 cut(s) 38, 409, 444, 570, 689, 914, 1308, 1593, 1604
Hpy8I GTNNAC 5 cut(s) 57, 622, 673, 1302, 1439
HpyAV CCTTC 4 cut(s) 1000, 1288, 1537, 1543
HpyCH4III ACNGT 3 cut(s) 160, 902, 1459
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 6 cut(s) 744, 795, 833, 947, 976, 1323
HpyF10VI GCNNNNNNNGC 3 cut(s) 934, 1102, 1320
HpySE526I ACGT 1 cut(s) 336
Hsp92I GRCGYC 1 cut(s) 1268
Kzo9I GATC 9 cut(s) 289, 354, 376, 516, 1009, 1229, 1331, 1335, 1379
LguI GCTCTTC 3 cut(s) 172, 553, 918
LmnI GCTCC 1 cut(s) 1173
Lsp1109I GCAGC 2 cut(s) 1597, 1600
LweI GCATC 6 cut(s) 242, 634, 842, 1332, 1516, 1597
MaeI CTAG 6 cut(s) 444, 689, 731, 960, 1083, 1106
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 2 cut(s) 154, 726
MalI GATC 9 cut(s) 291, 356, 378, 518, 1011, 1231, 1333, 1337, 1381
MboI GATC 9 cut(s) 289, 354, 376, 516, 1009, 1229, 1331, 1335, 1379
MflI RGATCY 3 cut(s) 354, 516, 1379
MhlI GDGCHC 3 cut(s) 1197, 1576, 1600
MluCI AATT 7 cut(s) 370, 422, 577, 762, 781, 894, 909
MlyI GAGTC 3 cut(s) 43, 575, 1307
MmeI TCCRAC 1 cut(s) 1055
Mph1103I ATGCAT 1 cut(s) 1325
MroXI GAANNNNTTC 2 cut(s) 577, 927
MseI TTAA 5 cut(s) 198, 207, 681, 897, 1503
MslI CAYNNNNRTG 1 cut(s) 510
MspI CCGG 3 cut(s) 98, 118, 428
MspR9I CCNGG 2 cut(s) 540, 1045
Mva1269I GAATGC 1 cut(s) 939
MvaI CCWGG 2 cut(s) 540, 1045
MwoI GCNNNNNNNGC 3 cut(s) 934, 1102, 1320
NcoI CCATGG 1 cut(s) 322
NdeII GATC 9 cut(s) 289, 354, 376, 516, 1009, 1229, 1331, 1335, 1379
NlaIV GGNNCC 2 cut(s) 122, 432
NmuCI GTSAC 1 cut(s) 726
NsiI ATGCAT 1 cut(s) 1325
NspI RCATGY 1 cut(s) 699
NspV TTCGAA 1 cut(s) 931
PciI ACATGT 1 cut(s) 695
PciSI GCTCTTC 3 cut(s) 172, 553, 918
PctI GAATGC 1 cut(s) 939
PdmI GAANNNNTTC 2 cut(s) 577, 927
PflMI CCANNNNNTGG 1 cut(s) 323
PkrI GCNGC 3 cut(s) 1612, 1615, 1625
Ple19I CGATCG 1 cut(s) 1232
PleI GAGTC 3 cut(s) 42, 574, 1306
PpsI GAGTC 3 cut(s) 42, 574, 1306
PscI ACATGT 1 cut(s) 695
PshBI ATTAAT 1 cut(s) 198
Psp124BI GAGCTC 1 cut(s) 1576
Psp6I CCWGG 2 cut(s) 538, 1043
PspFI CCCAGC 1 cut(s) 1096
PspGI CCWGG 2 cut(s) 538, 1043
PspN4I GGNNCC 2 cut(s) 122, 432
PspPI GGNCC 1 cut(s) 57
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 3 cut(s) 354, 516, 1379
PvuI CGATCG 1 cut(s) 1232
RsaI GTAC 5 cut(s) 335, 614, 700, 969, 1440
RsaNI GTAC 5 cut(s) 334, 613, 699, 968, 1439
RseI CAYNNNNRTG 1 cut(s) 510
SacI GAGCTC 1 cut(s) 1576
SapI GCTCTTC 3 cut(s) 172, 553, 918
SaqAI TTAA 5 cut(s) 198, 207, 681, 897, 1503
SatI GCNGC 3 cut(s) 1611, 1614, 1624
Sau3AI GATC 9 cut(s) 289, 354, 376, 516, 1009, 1229, 1331, 1335, 1379
Sau96I GGNCC 1 cut(s) 57
SchI GAGTC 3 cut(s) 43, 575, 1307
ScrFI CCNGG 2 cut(s) 540, 1045
SduI GDGCHC 3 cut(s) 1197, 1576, 1600
SfaNI GCATC 6 cut(s) 242, 634, 842, 1332, 1516, 1597
SfuI TTCGAA 1 cut(s) 931
SinI GGWCC 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 510
SmlI CTYRAG 4 cut(s) 171, 826, 953, 1196
SmoI CTYRAG 4 cut(s) 171, 826, 953, 1196
Sse9I AATT 7 cut(s) 370, 422, 577, 762, 781, 894, 909
SspI AATATT 1 cut(s) 1163
SspMI CTAG 6 cut(s) 444, 689, 731, 960, 1083, 1106
SstI GAGCTC 1 cut(s) 1576
StyD4I CCNGG 2 cut(s) 538, 1043
StyI CCWWGG 4 cut(s) 322, 451, 1127, 1400
TaaI ACNGT 3 cut(s) 160, 902, 1459
TaiI ACGT 1 cut(s) 339
TaqI TCGA 4 cut(s) 379, 931, 1334, 1563
TaqII GACCGA 2 cut(s) 74, 774
TasI AATT 7 cut(s) 370, 422, 577, 762, 781, 894, 909
TatI WGTACW 4 cut(s) 612, 698, 967, 1438
Tru1I TTAA 5 cut(s) 198, 207, 681, 897, 1503
Tru9I TTAA 5 cut(s) 198, 207, 681, 897, 1503
TscAI CASTG 5 cut(s) 301, 559, 802, 1008, 1174
TseFI GTSAC 1 cut(s) 726
TseI GCWGC 3 cut(s) 1610, 1613, 1623
Tsp45I GTSAC 1 cut(s) 726
TspDTI ATGAA 9 cut(s) 29, 136, 234, 378, 453, 522, 828, 975, 1227
TspGWI ACGGA 1 cut(s) 1378
TspRI CASTG 5 cut(s) 301, 559, 802, 1008, 1174
Van91I CCANNNNNTGG 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 57
VspI ATTAAT 1 cut(s) 198
XapI RAATTY 4 cut(s) 577, 762, 781, 909
XbaI TCTAGA 2 cut(s) 443, 688
XceI RCATGY 1 cut(s) 699
XmiI GTMKAC 1 cut(s) 1301
XmnI GAANNNNTTC 2 cut(s) 577, 927
XspI CTAG 6 cut(s) 444, 689, 731, 960, 1083, 1106
Zsp2I ATGCAT 1 cut(s) 1325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.