AT2G28480

CRS1_YhbY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015258
Physical Location & Seq
Reverse (-)
12176483 .. 12178498
2016 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G28480.1

Sequence Viewer

Length: 1119 bp
ATGGCAATTGCTTTTGCTCGTGGGTTGCGAAAAGCTTCATCTTTTCTTCGATCTTCATATCCACTTCTCATTTCTTCCAGGTTGCTTCCTATAAGCCAAGAGTTTTATGATTCAACATTGACATTTGTTCTACCGACAAGACCAATATGTAGGAATTTTAGTCATGGTTATGGTACGGTGAATCTAGTGATATCAGAAGGGAAACCAAAGTTTGAAACTAGGGAACTTGATCCTCCAAAGAAGTATAAGTGGTTGACGAAAAAGCGGTTGAAGTTGAAGAGAAAGAAAGAAAGAGAGGAAAGGAATGCAGCTAATAGGAAAGACCCGAGGCGACTTACTGTTAAAGGGAAGAAGAAGAAGTTTGTTAATGCAGAGGAAAGAATCAAGTATAAGCTTGAAAAGGCTAAGATCAAAGAAGCATTGCTGATTGAGAGACTTAAACGGTATGAAGTTGCAAAAGTGCAAGGACCTGAGGTTCGACCTCATGAGATTACCGGGGAAGAACGTTTCTACTTGAAGAAAATGGGTCAGAAAAGATCCAATTATGTACCGATTGGTAGAAGAGGAGTATTTGGTGGTGTTATTCTGAACATGCATCTGCATTGGAAAAAGCATGAAACTGTTAAGGTTATTTGCAATAATAGTAAGCCCGGCCAAGTGCAACAATATGCAGAGGAGCTTGCTAAACTGAGTGGTGGTGTGCCTGTTAACATAATTGGCGATGATACGATCATATTTTATAGAGGGAAGGGTTATGTGCAGCCACAAGTTATGTCTCCGATCGATACATTATCGAAGAAAAGGGCATATGAAAAATCGAAGTACGAACAATCTCTTGAATCTGTGAGACATTTTATAGCAATTGCAGAGAAGGAACTTGAACTATATTACAGGCATGTTGCTCTCTACGATGACCCGAATAACAGAAACCCTCTTTCGATTTTGGATGATTCTCCATCGGAATCACGTCAACACCATGAGAATGAGTTTTTTCTGAGTTGTTCGGATACCGACTCTAGTTCCGAAGATGAAGATGAAGATGAAGAGCTCTGTGAATTAGATAATGGTTCTTCTTCTTCTTCTTCTGCAAAGGAAGAGTTGTCTGAAAGTGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

372

Amino Acids

42.96

Weight (kDa)

9.3

Isoelectric Point (pI)

57.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 164 - 248 1.4e-20 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28480
fragaria_vesca FvH4_4g10890
malus_domestica MD04G1042300.v1.1
prunus_persica Prupe.1G196500_v2.0.a1
pyrus_communis pycom04g03720 pycom13g28110
rosa_chinensis RchiOBHm_Chr4g0406531
rosa_laevigata RLG00000008767
rosa_multiflora Rmu_ssc0000186.1_g000035
rosa_roxburghii Rroxscaffold_5G00351420
rosa_rugosa Rorug04G0072100
rosa_samantha Rh4AG139100 Rh4BG136200 Rh4CG146400 Rh4DG133500
rosa_wichuraiana Rw4G011350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 265
AclI AACGTT 1 cut(s) 505
AclWI GGATC 2 cut(s) 224, 531
AcoI YGGCCR 1 cut(s) 652
AcsI RAATTY 1 cut(s) 154
AfaI GTAC 3 cut(s) 175, 549, 824
AgsI TTSAA 8 cut(s) 114, 215, 271, 277, 398, 517, 839, 881
AjiI CACGTC 1 cut(s) 968
AjnI CCWGG 1 cut(s) 77
AloI GAACNNNNNNTCC 2 cut(s) 459, 491
AluBI AGCT 5 cut(s) 35, 311, 394, 679, 1048
AluI AGCT 5 cut(s) 35, 311, 394, 679, 1048
Alw21I GWGCWC 1 cut(s) 1050
Alw26I GTCTC 3 cut(s) 427, 780, 841
AlwI GGATC 2 cut(s) 224, 531
Ama87I CYCGRG 1 cut(s) 325
AoxI GGCC 1 cut(s) 652
ApeKI GCWGC 2 cut(s) 308, 760
ApoI RAATTY 1 cut(s) 154
ArsI GACNNNNNNTTYG 2 cut(s) 459, 491
Asp700I GAANNNNTTC 1 cut(s) 34
AspS9I GGNCC 1 cut(s) 467
AsuC2I CCSGG 2 cut(s) 496, 651
AsuHPI GGTGA 1 cut(s) 190
AvaI CYCGRG 1 cut(s) 325
AvaII GGWCC 1 cut(s) 467
AxyI CCTNAGG 1 cut(s) 471
BanII GRGCYC 1 cut(s) 1050
BauI CACGAG 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 1050
BbvI GCAGC 2 cut(s) 320, 772
BccI CCATC 1 cut(s) 964
BciT130I CCWGG 1 cut(s) 79
BciVI GTATCC 1 cut(s) 1000
BcnI CCSGG 2 cut(s) 496, 651
BcoDI GTCTC 3 cut(s) 427, 780, 841
BfaI CTAG 3 cut(s) 185, 219, 1017
BfuI GTATCC 1 cut(s) 1000
BisI GCNGC 2 cut(s) 309, 761
BlsI GCNGC 2 cut(s) 310, 762
Bme1390I CCNGG 3 cut(s) 79, 496, 651
Bme18I GGWCC 1 cut(s) 467
BmeT110I CYCGRG 1 cut(s) 325
BmgBI CACGTC 1 cut(s) 968
BmgT120I GGNCC 1 cut(s) 467
BmrFI CCNGG 3 cut(s) 79, 496, 651
BmsI GCATC 1 cut(s) 604
BpuMI CCSGG 2 cut(s) 496, 651
Bsa29I ATCGAT 1 cut(s) 783
BsaJI CCNNGG 2 cut(s) 326, 495
Bse21I CCTNAGG 1 cut(s) 471
Bse3DI GCAATG 1 cut(s) 419
BseBI CCWGG 1 cut(s) 79
BseCI ATCGAT 1 cut(s) 783
BseDI CCNNGG 2 cut(s) 326, 495
BseGI GGATG 1 cut(s) 952
BseMI GCAATG 1 cut(s) 419
BseMII CTCAG 3 cut(s) 462, 680, 986
BseRI GAGGAG 2 cut(s) 579, 689
BseXI GCAGC 2 cut(s) 320, 772
BsgI GTGCAG 1 cut(s) 779
Bsh1285I CGRYCG 1 cut(s) 783
BshFI GGCC 1 cut(s) 654
BshVI ATCGAT 1 cut(s) 783
BsiEI CGRYCG 1 cut(s) 783
BsiHKAI GWGCWC 1 cut(s) 1050
BsiHKCI CYCGRG 1 cut(s) 325
BsiSI CCGG 2 cut(s) 495, 651
BsmAI GTCTC 3 cut(s) 427, 780, 841
BsmI GAATGC 1 cut(s) 310
BsnI GGCC 1 cut(s) 654
BsoBI CYCGRG 1 cut(s) 325
Bsp1286I GDGCHC 1 cut(s) 1050
Bsp143I GATC 6 cut(s) 50, 229, 408, 536, 729, 780
BspACI CCGC 1 cut(s) 265
BspANI GGCC 1 cut(s) 654
BspCNI CTCAG 3 cut(s) 463, 681, 987
BspDI ATCGAT 1 cut(s) 783
BspHI TCATGA 1 cut(s) 484
BspPI GGATC 2 cut(s) 224, 531
BspQI GCTCTTC 1 cut(s) 1038
BsrDI GCAATG 1 cut(s) 419
BssECI CCNNGG 2 cut(s) 326, 495
BssMI GATC 6 cut(s) 50, 229, 408, 536, 729, 780
BssSI CACGAG 1 cut(s) 18
Bst2BI CACGAG 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 79
Bst4CI ACNGT 4 cut(s) 178, 340, 444, 622
Bst6I CTCTTC 4 cut(s) 272, 556, 1038, 1089
BstC8I GCNNGC 1 cut(s) 681
BstDEI CTNAG 4 cut(s) 405, 471, 689, 995
BstF5I GGATG 1 cut(s) 952
BstKTI GATC 6 cut(s) 53, 232, 411, 539, 732, 783
BstMAI GTCTC 3 cut(s) 427, 780, 841
BstMBI GATC 6 cut(s) 50, 229, 408, 536, 729, 780
BstMCI CGRYCG 1 cut(s) 783
BstNI CCWGG 1 cut(s) 79
BstNSI RCATGY 2 cut(s) 595, 899
BstSCI CCNGG 3 cut(s) 77, 494, 649
BstV1I GCAGC 2 cut(s) 320, 772
BstX2I RGATCY 1 cut(s) 536
BstYI RGATCY 1 cut(s) 536
Bsu15I ATCGAT 1 cut(s) 783
Bsu36I CCTNAGG 1 cut(s) 471
BsuI GTATCC 1 cut(s) 1000
BsuRI GGCC 1 cut(s) 654
BsuTUI ATCGAT 1 cut(s) 783
BtgZI GCGATG 1 cut(s) 735
BtrI CACGTC 1 cut(s) 968
BtsCI GGATG 1 cut(s) 952
Cac8I GCNNGC 1 cut(s) 681
CciI TCATGA 1 cut(s) 484
Cfr13I GGNCC 1 cut(s) 467
ClaI ATCGAT 1 cut(s) 783
Csp6I GTAC 3 cut(s) 174, 548, 823
CviAII CATG 6 cut(s) 164, 485, 592, 614, 896, 977
CviQI GTAC 3 cut(s) 174, 548, 823
DdeI CTNAG 4 cut(s) 405, 471, 689, 995
DpnI GATC 6 cut(s) 52, 231, 410, 538, 731, 782
DpnII GATC 6 cut(s) 50, 229, 408, 536, 729, 780
EaeI YGGCCR 1 cut(s) 652
Eam1104I CTCTTC 4 cut(s) 272, 556, 1038, 1089
EarI CTCTTC 4 cut(s) 272, 556, 1038, 1089
Ecl136II GAGCTC 1 cut(s) 1048
Eco24I GRGCYC 1 cut(s) 1050
Eco32I GATATC 1 cut(s) 192
Eco47I GGWCC 1 cut(s) 467
Eco53kI GAGCTC 1 cut(s) 1048
Eco81I CCTNAGG 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 325
EcoICRI GAGCTC 1 cut(s) 1048
EcoO109I RGGNCCY 1 cut(s) 467
EcoRII CCWGG 1 cut(s) 77
EcoRV GATATC 1 cut(s) 192
EcoT22I ATGCAT 1 cut(s) 597
EcoT38I GRGCYC 1 cut(s) 1050
FaeI CATG 6 cut(s) 167, 488, 595, 617, 899, 980
FatI CATG 6 cut(s) 163, 484, 591, 613, 895, 976
FauNDI CATATG 1 cut(s) 808
Fnu4HI GCNGC 2 cut(s) 309, 761
FokI GGATG 1 cut(s) 959
FriOI GRGCYC 1 cut(s) 1050
Fsp4HI GCNGC 2 cut(s) 309, 761
FspBI CTAG 3 cut(s) 185, 219, 1017
GluI GCNGC 2 cut(s) 309, 761
HaeIII GGCC 1 cut(s) 654
HapII CCGG 2 cut(s) 495, 651
Hin1II CATG 6 cut(s) 167, 488, 595, 617, 899, 980
HincII GTYRAC 3 cut(s) 255, 709, 971
HindII GTYRAC 3 cut(s) 255, 709, 971
HindIII AAGCTT 2 cut(s) 33, 392
HinfI GANTC 7 cut(s) 110, 181, 381, 839, 950, 962, 1013
HpaI GTTAAC 1 cut(s) 709
HpaII CCGG 2 cut(s) 495, 651
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 3 cut(s) 255, 709, 971
Hpy188I TCNGA 9 cut(s) 196, 531, 588, 780, 961, 996, 1006, 1024, 1105
Hpy188III TCNNGA 2 cut(s) 485, 836
Hpy8I GTNNAC 3 cut(s) 255, 709, 971
HpyAV CCTTC 3 cut(s) 191, 742, 865
HpyCH4III ACNGT 4 cut(s) 178, 340, 444, 622
HpyCH4IV ACGT 2 cut(s) 505, 967
HpyF3I CTNAG 4 cut(s) 405, 471, 689, 995
HpySE526I ACGT 2 cut(s) 505, 967
Hsp92II CATG 6 cut(s) 167, 488, 595, 617, 899, 980
KspAI GTTAAC 1 cut(s) 709
Kzo9I GATC 6 cut(s) 50, 229, 408, 536, 729, 780
LguI GCTCTTC 1 cut(s) 1038
LmnI GCTCC 1 cut(s) 676
LpnPI CCDG 7 cut(s) 64, 91, 483, 508, 664, 717, 877
Lsp1109I GCAGC 2 cut(s) 320, 772
LweI GCATC 1 cut(s) 604
MaeI CTAG 3 cut(s) 185, 219, 1017
MaeII ACGT 2 cut(s) 505, 967
MalI GATC 6 cut(s) 52, 231, 410, 538, 731, 782
MboI GATC 6 cut(s) 50, 229, 408, 536, 729, 780
MfeI CAATTG 2 cut(s) 6, 861
MflI RGATCY 1 cut(s) 536
MhlI GDGCHC 1 cut(s) 1050
MluCI AATT 6 cut(s) 6, 154, 541, 714, 861, 1055
MlyI GAGTC 1 cut(s) 1007
Mph1103I ATGCAT 1 cut(s) 597
MroXI GAANNNNTTC 1 cut(s) 34
MseI TTAA 5 cut(s) 342, 366, 438, 624, 708
MslI CAYNNNNRTG 2 cut(s) 168, 981
MspI CCGG 2 cut(s) 495, 651
MspR9I CCNGG 3 cut(s) 79, 496, 651
MunI CAATTG 2 cut(s) 6, 861
Mva1269I GAATGC 1 cut(s) 310
MvaI CCWGG 1 cut(s) 79
NciI CCSGG 2 cut(s) 496, 651
NdeI CATATG 1 cut(s) 808
NdeII GATC 6 cut(s) 50, 229, 408, 536, 729, 780
NlaIII CATG 6 cut(s) 167, 488, 595, 617, 899, 980
NsiI ATGCAT 1 cut(s) 597
NspI RCATGY 2 cut(s) 595, 899
PagI TCATGA 1 cut(s) 484
PciSI GCTCTTC 1 cut(s) 1038
PcsI WCGNNNNNNNCGW 1 cut(s) 25
PctI GAATGC 1 cut(s) 310
PdmI GAANNNNTTC 1 cut(s) 34
PfeI GAWTC 6 cut(s) 110, 181, 381, 839, 950, 962
PkrI GCNGC 2 cut(s) 310, 762
Ple19I CGATCG 1 cut(s) 783
PleI GAGTC 1 cut(s) 1007
PpsI GAGTC 1 cut(s) 1007
PpuMI RGGWCCY 1 cut(s) 467
Psp124BI GAGCTC 1 cut(s) 1050
Psp1406I AACGTT 1 cut(s) 505
Psp5II RGGWCCY 1 cut(s) 467
Psp6I CCWGG 1 cut(s) 77
PspGI CCWGG 1 cut(s) 77
PspPI GGNCC 1 cut(s) 467
PspPPI RGGWCCY 1 cut(s) 467
PsuI RGATCY 1 cut(s) 536
PvuI CGATCG 1 cut(s) 783
RsaI GTAC 3 cut(s) 175, 549, 824
RsaNI GTAC 3 cut(s) 174, 548, 823
RseI CAYNNNNRTG 2 cut(s) 168, 981
SacI GAGCTC 1 cut(s) 1050
SapI GCTCTTC 1 cut(s) 1038
SaqAI TTAA 5 cut(s) 342, 366, 438, 624, 708
SatI GCNGC 2 cut(s) 309, 761
Sau3AI GATC 6 cut(s) 50, 229, 408, 536, 729, 780
Sau96I GGNCC 1 cut(s) 467
SchI GAGTC 1 cut(s) 1007
ScrFI CCNGG 3 cut(s) 79, 496, 651
SduI GDGCHC 1 cut(s) 1050
SfaNI GCATC 1 cut(s) 604
SinI GGWCC 1 cut(s) 467
SmiMI CAYNNNNRTG 2 cut(s) 168, 981
Sse9I AATT 6 cut(s) 6, 154, 541, 714, 861, 1055
SsiI CCGC 1 cut(s) 265
SspMI CTAG 3 cut(s) 185, 219, 1017
SstI GAGCTC 1 cut(s) 1050
StyD4I CCNGG 3 cut(s) 77, 494, 649
TaaI ACNGT 4 cut(s) 178, 340, 444, 622
TaiI ACGT 2 cut(s) 508, 970
TaqI TCGA 6 cut(s) 49, 478, 783, 794, 818, 938
TasI AATT 6 cut(s) 6, 154, 541, 714, 861, 1055
TfiI GAWTC 6 cut(s) 110, 181, 381, 839, 950, 962
Tru1I TTAA 5 cut(s) 342, 366, 438, 624, 708
Tru9I TTAA 5 cut(s) 342, 366, 438, 624, 708
TseI GCWGC 2 cut(s) 308, 760
TspDTI ATGAA 8 cut(s) 27, 45, 462, 630, 825, 1044, 1050, 1056
VpaK11BI GGWCC 1 cut(s) 467
XapI RAATTY 1 cut(s) 154
XceI RCATGY 2 cut(s) 595, 899
XmnI GAANNNNTTC 1 cut(s) 34
XspI CTAG 3 cut(s) 185, 219, 1017
Zsp2I ATGCAT 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.