AT2G31350

hydroxyacylglutathione hydrolase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011321
Physical Location & Seq
Forward (+)
13368070 .. 13371097
3028 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G31350.2

Sequence Viewer

Length: 975 bp
ATGCAAACCATCTCGAAAGCTTCTTCTGCCACCTCCTTTTTTCGATGTTCTAGGAAGCTATCAAGCCAGCCATGTGTGAGACAGCTTAACATAAGAAAGAGTCTTGTGTGCAGAGTGATGAAGCTGGTCTCTTCACCTCTTAGGACTTTGCGTGGTGCTGGTAAATCTATCCGAGTTTCAAAGTTCTGCAGTGTCTCCAATGTCTCCTCATTGCAAATTGAACTGGTGCCTTGCCTTAAGGATAACTATGCTTATATCTTGCATGACGAGGATACTGGTACAGTTGGGGTGGTTGACCCTTCTGAAGCTGAACCTATTATCGATTCGTTGAAGAGGAGTGGTCGTAATCTAACATATATATTAAATACACATCATCATTATGATCACACTGGTGGGAATTTGGAATTAAAAGACAGGTACGGGGCAAAGGTGATTGGCTCAGCTATGGATAAAGACCGGATTCCTGGAATTGATATGGCCTTGAAGGATGGTGACAAATGGATGTTTGCTGGCCATGAAGTCCATGTTATGGATACTCCTGGCCACACAAAAGGCCATATCAGCTTATACTTTCCAGGATCACGAGCGATCTTCACCGGGGACACGATGTTTAGCTTATCATGTGGTAAACTCTTTGAAGGCACTCCTAAGCAGATGCTTGCTTCTCTCCAAAAGATCACATCTTTACCAGATGACACAAGCATATACTGTGGTCATGAATATACACTGAGTAATTCCAAGTTTGCGTTGTCTCTAGAGCCAAACAACGAAGTACTTCAGTCCTATGCAGCTCATGTTGCAGAGCTCCGAAGCAAGAAATTACCAACGATTCCGACAACAGTGAAGATGGAGAAAGCTTGTAACCCCTTCCTCCGCAGCTCAAATACAGATATCCGTCGGGCTTTACGCATTCCAGAGGCCGCAGATGAAGCAGAAGCTTTAGGGATTATCCGGAAAGCAAAGGATGATTTCTAA

Protein Analysis

324

Amino Acids

35.84

Weight (kDa)

9.0

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 90 - 238 4.4e-22 Metallo-beta-lactamase superfamily
HAGH_C PF16123 239 - 323 3.1e-24 Hydroxyacylglutathione hydrolase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011321)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06130 AT1G06130 AT2G31350 AT2G43430 AT2G43430
fragaria_vesca FvH4_3g41770 FvH4_3g41770
malus_domestica MD04G1045200.v1.1
prunus_persica Prupe.1G198300_v2.0.a1 Prupe.1G198300_v2.0.a1
pyrus_communis pycom04g03890
rosa_chinensis RchiOBHm_Chr5g0074861
rosa_laevigata RLG00000036511
rosa_multiflora Rmu_sc0003945.1_g000008 Rmu_sc0012777.1_g000001
rosa_roxburghii Rroxscaffold_1G00006720
rosa_rugosa Rorug05G0435000
rosa_samantha Rh5CG537400 Rh5DG525800
rosa_wichuraiana Rw5G045610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 226
AccB7I CCANNNNNTGG 1 cut(s) 529
AccIII TCCGGA 1 cut(s) 951
AciI CCGC 2 cut(s) 874, 921
AclWI GGATC 1 cut(s) 586
AcoI YGGCCR 2 cut(s) 511, 541
AcsI RAATTY 1 cut(s) 397
AcuI CTGAAG 2 cut(s) 324, 761
AfaI GTAC 3 cut(s) 280, 419, 774
AfiI CCNNNNNNNGG 1 cut(s) 529
AflII CTTAAG 1 cut(s) 236
AgsI TTSAA 5 cut(s) 180, 221, 331, 484, 638
AjnI CCWGG 3 cut(s) 463, 538, 574
AleI CACNNNNGTG 1 cut(s) 390
Alw21I GWGCWC 1 cut(s) 807
Alw26I GTCTC 5 cut(s) 73, 133, 199, 208, 756
AlwI GGATC 1 cut(s) 586
Aor13HI TCCGGA 1 cut(s) 951
AoxI GGCC 5 cut(s) 477, 511, 541, 553, 918
ApeKI GCWGC 2 cut(s) 788, 876
ApoI RAATTY 1 cut(s) 397
Asp700I GAANNNNTTC 1 cut(s) 774
AsuC2I CCSGG 1 cut(s) 598
AsuHPI GGTGA 4 cut(s) 126, 442, 503, 586
BalI TGGCCA 2 cut(s) 513, 543
BanI GGYRCC 1 cut(s) 226
BanII GRGCYC 1 cut(s) 807
BauI CACGAG 1 cut(s) 582
Bbv12I GWGCWC 1 cut(s) 807
BbvI GCAGC 2 cut(s) 800, 888
BccI CCATC 3 cut(s) 17, 482, 841
BciT130I CCWGG 3 cut(s) 465, 540, 576
BciVI GTATCC 2 cut(s) 265, 526
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 1 cut(s) 598
BcoDI GTCTC 5 cut(s) 73, 133, 199, 208, 756
BfaI CTAG 2 cut(s) 51, 755
BfmI CTRYAG 1 cut(s) 187
BfrI CTTAAG 1 cut(s) 236
BfuI GTATCC 2 cut(s) 265, 526
BisI GCNGC 3 cut(s) 789, 877, 921
BlpI GCTNAGC 1 cut(s) 439
BlsI GCNGC 3 cut(s) 790, 878, 922
BmcAI AGTACT 1 cut(s) 774
Bme1390I CCNGG 4 cut(s) 465, 540, 576, 598
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 4 cut(s) 465, 540, 576, 598
BmsI GCATC 1 cut(s) 645
Bpu10I CCTNAGC 1 cut(s) 648
Bpu1102I GCTNAGC 1 cut(s) 439
BpuMI CCSGG 1 cut(s) 598
Bsa29I ATCGAT 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 133
BsaJI CCNNGG 1 cut(s) 597
BsaWI WCCGGW 2 cut(s) 456, 951
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bsc4I CCNNNNNNNGG 1 cut(s) 529
Bse1I ACTGG 3 cut(s) 228, 280, 394
Bse3DI GCAATG 1 cut(s) 209
BseAI TCCGGA 1 cut(s) 951
BseBI CCWGG 3 cut(s) 465, 540, 576
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 1 cut(s) 597
BseGI GGATG 3 cut(s) 493, 507, 970
BseLI CCNNNNNNNGG 1 cut(s) 529
BseMI GCAATG 1 cut(s) 209
BseMII CTCAG 2 cut(s) 453, 719
BseNI ACTGG 3 cut(s) 228, 280, 394
BseRI GAGGAG 2 cut(s) 196, 349
BseXI GCAGC 2 cut(s) 800, 888
BsgI GTGCAG 1 cut(s) 130
BshFI GGCC 5 cut(s) 479, 513, 543, 555, 920
BshNI GGYRCC 1 cut(s) 226
BshVI ATCGAT 1 cut(s) 321
BsiHKAI GWGCWC 1 cut(s) 807
BsiSI CCGG 3 cut(s) 457, 597, 952
BslFI GGGAC 1 cut(s) 614
BslI CCNNNNNNNGG 1 cut(s) 529
BsmAI GTCTC 5 cut(s) 73, 133, 199, 208, 756
BsmFI GGGAC 1 cut(s) 614
BsmI GAATGC 1 cut(s) 909
BsnI GGCC 5 cut(s) 479, 513, 543, 555, 920
Bso31I GGTCTC 1 cut(s) 133
Bsp1286I GDGCHC 1 cut(s) 807
Bsp13I TCCGGA 1 cut(s) 951
Bsp143I GATC 4 cut(s) 382, 578, 588, 675
Bsp1720I GCTNAGC 1 cut(s) 439
BspACI CCGC 2 cut(s) 874, 921
BspANI GGCC 5 cut(s) 479, 513, 543, 555, 920
BspCNI CTCAG 2 cut(s) 452, 720
BspDI ATCGAT 1 cut(s) 321
BspEI TCCGGA 1 cut(s) 951
BspHI TCATGA 1 cut(s) 715
BspLI GGNNCC 1 cut(s) 228
BspMAI CTGCAG 1 cut(s) 191
BspPI GGATC 1 cut(s) 586
BspT107I GGYRCC 1 cut(s) 226
BspTI CTTAAG 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 133
BsrDI GCAATG 1 cut(s) 209
BsrI ACTGG 3 cut(s) 228, 280, 394
BssECI CCNNGG 1 cut(s) 597
BssMI GATC 4 cut(s) 382, 578, 588, 675
BssSI CACGAG 1 cut(s) 582
Bst2BI CACGAG 1 cut(s) 582
Bst2UI CCWGG 3 cut(s) 465, 540, 576
Bst4CI ACNGT 3 cut(s) 283, 710, 841
Bst6I CTCTTC 2 cut(s) 136, 326
BstAFI CTTAAG 1 cut(s) 236
BstC8I GCNNGC 3 cut(s) 68, 511, 660
BstDEI CTNAG 4 cut(s) 140, 439, 648, 728
BstF5I GGATG 3 cut(s) 493, 507, 970
BstKTI GATC 4 cut(s) 385, 581, 591, 678
BstMAI GTCTC 5 cut(s) 73, 133, 199, 208, 756
BstMBI GATC 4 cut(s) 382, 578, 588, 675
BstMWI GCNNNNNNNGC 4 cut(s) 26, 561, 797, 929
BstNI CCWGG 3 cut(s) 465, 540, 576
BstSCI CCNGG 4 cut(s) 463, 538, 574, 596
BstSFI CTRYAG 1 cut(s) 187
BstV1I GCAGC 2 cut(s) 800, 888
Bsu15I ATCGAT 1 cut(s) 321
BsuI GTATCC 2 cut(s) 265, 526
BsuRI GGCC 5 cut(s) 479, 513, 543, 555, 920
BsuTUI ATCGAT 1 cut(s) 321
BtsCI GGATG 3 cut(s) 493, 507, 970
BtsI GCAGTG 1 cut(s) 196
BtsIMutI CAGTG 4 cut(s) 196, 387, 725, 846
Cac8I GCNNGC 3 cut(s) 68, 511, 660
CciI TCATGA 1 cut(s) 715
ClaI ATCGAT 1 cut(s) 321
Csp6I GTAC 3 cut(s) 279, 418, 773
CviAII CATG 7 cut(s) 72, 263, 515, 524, 621, 716, 794
CviQI GTAC 3 cut(s) 279, 418, 773
DdeI CTNAG 4 cut(s) 140, 439, 648, 728
DpnI GATC 4 cut(s) 384, 580, 590, 677
DpnII GATC 4 cut(s) 382, 578, 588, 675
EaeI YGGCCR 2 cut(s) 511, 541
Eam1104I CTCTTC 2 cut(s) 136, 326
EarI CTCTTC 2 cut(s) 136, 326
Ecl136II GAGCTC 1 cut(s) 805
Eco24I GRGCYC 1 cut(s) 807
Eco31I GGTCTC 1 cut(s) 133
Eco32I GATATC 1 cut(s) 892
Eco53kI GAGCTC 1 cut(s) 805
Eco57I CTGAAG 2 cut(s) 324, 761
EcoICRI GAGCTC 1 cut(s) 805
EcoRII CCWGG 3 cut(s) 463, 538, 574
EcoRV GATATC 1 cut(s) 892
EcoT38I GRGCYC 1 cut(s) 807
FaeI CATG 7 cut(s) 75, 266, 518, 527, 624, 719, 797
FaqI GGGAC 1 cut(s) 614
FatI CATG 7 cut(s) 71, 262, 514, 523, 620, 715, 793
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 3 cut(s) 789, 877, 921
FokI GGATG 2 cut(s) 500, 514
FriOI GRGCYC 1 cut(s) 807
Fsp4HI GCNGC 3 cut(s) 789, 877, 921
FspBI CTAG 2 cut(s) 51, 755
GluI GCNGC 3 cut(s) 789, 877, 921
HaeIII GGCC 5 cut(s) 479, 513, 543, 555, 920
HapII CCGG 3 cut(s) 457, 597, 952
Hin1II CATG 7 cut(s) 75, 266, 518, 527, 624, 719, 797
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 3 cut(s) 18, 855, 936
HinfI GANTC 4 cut(s) 100, 323, 460, 829
HpaII CCGG 3 cut(s) 457, 597, 952
HphI GGTGA 4 cut(s) 126, 442, 503, 586
Hpy166II GTNNAC 2 cut(s) 295, 629
Hpy188I TCNGA 4 cut(s) 173, 304, 809, 834
Hpy188III TCNNGA 6 cut(s) 13, 582, 716, 755, 914, 952
Hpy8I GTNNAC 2 cut(s) 295, 629
Hpy99I CGWCG 1 cut(s) 900
HpyAV CCTTC 4 cut(s) 309, 478, 632, 877
HpyCH4III ACNGT 3 cut(s) 283, 710, 841
HpyCH4V TGCA 7 cut(s) 4, 111, 189, 214, 262, 788, 800
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 561, 797, 929
HpyF3I CTNAG 4 cut(s) 140, 439, 648, 728
Hsp92II CATG 7 cut(s) 75, 266, 518, 527, 624, 719, 797
Kpn2I TCCGGA 1 cut(s) 951
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 4 cut(s) 382, 578, 588, 675
LmnI GCTCC 1 cut(s) 810
Lsp1109I GCAGC 2 cut(s) 800, 888
LweI GCATC 1 cut(s) 645
MaeI CTAG 2 cut(s) 51, 755
MaeIII GTNAC 2 cut(s) 491, 860
MalI GATC 4 cut(s) 384, 580, 590, 677
MboI GATC 4 cut(s) 382, 578, 588, 675
MboII GAAGA 5 cut(s) 15, 123, 343, 583, 856
MhlI GDGCHC 1 cut(s) 807
MlsI TGGCCA 2 cut(s) 513, 543
MluCI AATT 6 cut(s) 216, 397, 404, 468, 733, 818
MluNI TGGCCA 2 cut(s) 513, 543
MlyI GAGTC 1 cut(s) 109
MmeI TCCRAC 1 cut(s) 857
MnlI CCTC 7 cut(s) 43, 147, 217, 262, 327, 881, 910
Mox20I TGGCCA 2 cut(s) 513, 543
MroI TCCGGA 1 cut(s) 951
MroXI GAANNNNTTC 1 cut(s) 774
MscI TGGCCA 2 cut(s) 513, 543
MseI TTAA 4 cut(s) 87, 237, 362, 407
MslI CAYNNNNRTG 2 cut(s) 378, 390
Msp20I TGGCCA 2 cut(s) 513, 543
MspCI CTTAAG 1 cut(s) 236
MspI CCGG 3 cut(s) 457, 597, 952
MspR9I CCNGG 4 cut(s) 465, 540, 576, 598
Mva1269I GAATGC 1 cut(s) 909
MvaI CCWGG 3 cut(s) 465, 540, 576
MwoI GCNNNNNNNGC 4 cut(s) 26, 561, 797, 929
NciI CCSGG 1 cut(s) 598
NdeII GATC 4 cut(s) 382, 578, 588, 675
NlaIII CATG 7 cut(s) 75, 266, 518, 527, 624, 719, 797
NlaIV GGNNCC 1 cut(s) 228
NmuCI GTSAC 1 cut(s) 491
OliI CACNNNNGTG 1 cut(s) 390
PagI TCATGA 1 cut(s) 715
PctI GAATGC 1 cut(s) 909
PdmI GAANNNNTTC 1 cut(s) 774
PfeI GAWTC 3 cut(s) 323, 460, 829
PflMI CCANNNNNTGG 1 cut(s) 529
PfoI TCCNGGA 2 cut(s) 463, 574
PkrI GCNGC 3 cut(s) 790, 878, 922
PleI GAGTC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 108
Psp124BI GAGCTC 1 cut(s) 807
Psp6I CCWGG 3 cut(s) 463, 538, 574
PspGI CCWGG 3 cut(s) 463, 538, 574
PspN4I GGNNCC 1 cut(s) 228
PstI CTGCAG 1 cut(s) 191
RsaI GTAC 3 cut(s) 280, 419, 774
RsaNI GTAC 3 cut(s) 279, 418, 773
RseI CAYNNNNRTG 2 cut(s) 378, 390
SacI GAGCTC 1 cut(s) 807
SaqAI TTAA 4 cut(s) 87, 237, 362, 407
SatI GCNGC 3 cut(s) 789, 877, 921
Sau3AI GATC 4 cut(s) 382, 578, 588, 675
ScaI AGTACT 1 cut(s) 774
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 4 cut(s) 465, 540, 576, 598
SduI GDGCHC 1 cut(s) 807
SfaNI GCATC 1 cut(s) 645
SfcI CTRYAG 1 cut(s) 187
SmiMI CAYNNNNRTG 2 cut(s) 378, 390
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
Sse9I AATT 6 cut(s) 216, 397, 404, 468, 733, 818
SsiI CCGC 2 cut(s) 874, 921
SspMI CTAG 2 cut(s) 51, 755
SstI GAGCTC 1 cut(s) 807
StyD4I CCNGG 4 cut(s) 463, 538, 574, 596
TaaI ACNGT 3 cut(s) 283, 710, 841
TaqI TCGA 3 cut(s) 14, 43, 321
TasI AATT 6 cut(s) 216, 397, 404, 468, 733, 818
TatI WGTACW 1 cut(s) 772
TauI GCSGC 1 cut(s) 923
TfiI GAWTC 3 cut(s) 323, 460, 829
Tru1I TTAA 4 cut(s) 87, 237, 362, 407
Tru9I TTAA 4 cut(s) 87, 237, 362, 407
TscAI CASTG 4 cut(s) 196, 394, 732, 846
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 2 cut(s) 788, 876
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 4 cut(s) 134, 531, 732, 942
TspGWI ACGGA 1 cut(s) 884
TspRI CASTG 4 cut(s) 196, 394, 732, 846
Van91I CCANNNNNTGG 1 cut(s) 529
Vha464I CTTAAG 1 cut(s) 236
XapI RAATTY 1 cut(s) 397
XbaI TCTAGA 1 cut(s) 754
XmnI GAANNNNTTC 1 cut(s) 774
XspI CTAG 2 cut(s) 51, 755
ZrmI AGTACT 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.