AT2G43430

Hydroxyacylglutathione hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011321
Physical Location & Seq
Reverse (-)
18035349 .. 18038334
2986 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G43430.2

Sequence Viewer

Length: 996 bp
ATGCCTGTGATCTCCAAAGCTTCATCTACCACCACCAATTCATCGATTCCTTCGTGTTCTAGGATTGGAGGTCAGCTTTGTGTGTGGCCTGGTTTGAGACAACTTTGCCTCAGGAAAAGCTTATTATACGGAGTAATGTGGTTATTATCCATGCCACTGAAAACATTGCGTGGAGCTAGAAAAACTCTTAAGATTACTCACTTTTGTAGCATTTCCAACATGCCATCCTCATTAAAAATCGAACTGGTGCCCTGCAGTAAGGACAACTATGCTTATCTGTTGCACGATGAAGACACCGGAACAGTTGGAGTCGTTGATCCTTCTGAGGCTGCACCTGTTATAGAAGCATTGAGCAGGAAGAATTGGAATTTAACTTACATTTTGAATACTCATCATCATGATGATCACATTGGGGGGAATGCTGAATTGAAAGAAAGGTATGGCGCAAAGGTGATTGGATCAGCTGTAGATAAAGATCGGATTCCTGGAATTGACATACTTCTGAAGGATAGTGATAAGTGGATGTTTGCTGGCCATGAGGTTCGGATACTTGACACTCCTGGCCATACACAAGGCCATATTAGCTTCTACTTTCCCGGGTCAGCGACAATATTCACAGGAGACCTGATATATAGCTTATCCTGTGGTACCCTTTCAGAAGGTACCCCTGAGCAGATGCTTTCATCACTCCAAAAGATCGTGTCTCTACCAGACGATACAAATATATACTGCGGCCGTGAAAACACAGCAGGCAATCTCAAGTTTGCTCTATCCGTAGAACCAAAGAATGAAACTCTTCAGTCCTATGCAACTCGAGTCGCCCATCTTCGCAGCCAGGGGCTCCCATCGATTCCAACGACTGTCAAGGTTGAGAAAGCGTGTAACCCATTCCTCAGAATATCGAGCAAAGATATCCGAAAATCTTTAAGCATTCCAGACTCAGCAACCGAAGCTGAAGCATTGCGTCGTATACAGAGAGCCAGAGATCGTTTCTGA

Protein Analysis

331

Amino Acids

36.5

Weight (kDa)

8.82

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 73 - 225 1.1e-20 Metallo-beta-lactamase superfamily
HAGH_C PF16123 229 - 313 1.3e-21 Hydroxyacylglutathione hydrolase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011321)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06130 AT1G06130 AT2G31350 AT2G43430 AT2G43430
fragaria_vesca FvH4_3g41770 FvH4_3g41770
malus_domestica MD04G1045200.v1.1
prunus_persica Prupe.1G198300_v2.0.a1 Prupe.1G198300_v2.0.a1
pyrus_communis pycom04g03890
rosa_chinensis RchiOBHm_Chr5g0074861
rosa_laevigata RLG00000036511
rosa_multiflora Rmu_sc0003945.1_g000008 Rmu_sc0012777.1_g000001
rosa_roxburghii Rroxscaffold_1G00006720
rosa_rugosa Rorug05G0435000
rosa_samantha Rh5CG537400 Rh5DG525800
rosa_wichuraiana Rw5G045610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 647, 662
AccB1I GGYRCC 3 cut(s) 247, 647, 662
AccI GTMKAC 1 cut(s) 970
AciI CCGC 1 cut(s) 732
AclWI GGATC 2 cut(s) 311, 466
AcoI YGGCCR 3 cut(s) 532, 562, 733
AcsI RAATTY 1 cut(s) 367
AcuI CTGAAG 3 cut(s) 524, 782, 975
AfaI GTAC 2 cut(s) 649, 664
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 2 cut(s) 385, 430
AjnI CCWGG 4 cut(s) 88, 484, 559, 834
AluBI AGCT 8 cut(s) 20, 76, 120, 176, 464, 585, 636, 953
AluI AGCT 8 cut(s) 20, 76, 120, 176, 464, 585, 636, 953
Alw26I GTCTC 3 cut(s) 91, 615, 708
AlwI GGATC 2 cut(s) 311, 466
Ama87I CYCGRG 2 cut(s) 596, 813
AoxI GGCC 5 cut(s) 86, 532, 562, 574, 733
ApeKI GCWGC 2 cut(s) 329, 831
ApoI RAATTY 1 cut(s) 367
Asp700I GAANNNNTTC 1 cut(s) 795
Asp718I GGTACC 2 cut(s) 647, 662
AspLEI GCGC 1 cut(s) 446
AsuC2I CCSGG 2 cut(s) 597, 598
AsuHPI GGTGA 1 cut(s) 463
AvaI CYCGRG 2 cut(s) 596, 813
AxyI CCTNAGG 1 cut(s) 110
BaeGI GKGCMC 1 cut(s) 252
BalI TGGCCA 2 cut(s) 534, 564
BanI GGYRCC 3 cut(s) 247, 647, 662
BanII GRGCYC 1 cut(s) 843
BbsI GAAGAC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 316, 843
BccI CCATC 3 cut(s) 232, 831, 853
BceAI ACGGC 1 cut(s) 720
BciT130I CCWGG 4 cut(s) 90, 486, 561, 836
BciVI GTATCC 1 cut(s) 540
BclI TGATCA 1 cut(s) 403
BcnI CCSGG 2 cut(s) 597, 598
BcoDI GTCTC 3 cut(s) 91, 615, 708
BfaI CTAG 2 cut(s) 60, 177
BfmI CTRYAG 2 cut(s) 253, 465
BfrI CTTAAG 1 cut(s) 188
BfuI GTATCC 1 cut(s) 540
BisI GCNGC 3 cut(s) 330, 733, 832
BlsI GCNGC 3 cut(s) 331, 734, 833
Bme1390I CCNGG 6 cut(s) 90, 486, 561, 597, 598, 836
BmeT110I CYCGRG 2 cut(s) 596, 813
BmiI GGNNCC 4 cut(s) 249, 649, 664, 842
BmrFI CCNGG 6 cut(s) 90, 486, 561, 597, 598, 836
BmsI GCATC 1 cut(s) 666
BpiI GAAGAC 1 cut(s) 297
Bpu10I CCTNAGC 1 cut(s) 669
BpuEI CTTGAG 1 cut(s) 743
BpuMI CCSGG 2 cut(s) 597, 598
Bsa29I ATCGAT 2 cut(s) 44, 848
BsaBI GATNNNNATC 1 cut(s) 474
BsaI GGTCTC 1 cut(s) 615
BsaJI CCNNGG 2 cut(s) 596, 835
BsaWI WCCGGW 1 cut(s) 296
Bse1I ACTGG 1 cut(s) 249
Bse21I CCTNAGG 1 cut(s) 110
Bse3DI GCAATG 2 cut(s) 164, 959
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 4 cut(s) 90, 486, 561, 836
BseCI ATCGAT 2 cut(s) 44, 848
BseDI CCNNGG 2 cut(s) 596, 835
BseGI GGATG 2 cut(s) 224, 528
BseJI GATNNNNATC 1 cut(s) 474
BseMI GCAATG 2 cut(s) 164, 959
BseMII CTCAG 5 cut(s) 124, 315, 660, 907, 954
BseNI ACTGG 1 cut(s) 249
BseSI GKGCMC 1 cut(s) 252
BseX3I CGGCCG 1 cut(s) 733
BseXI GCAGC 2 cut(s) 316, 843
BsgI GTGCAG 1 cut(s) 315
Bsh1285I CGRYCG 1 cut(s) 736
BshFI GGCC 5 cut(s) 88, 534, 564, 576, 735
BshNI GGYRCC 3 cut(s) 247, 647, 662
BshVI ATCGAT 2 cut(s) 44, 848
BsiEI CGRYCG 1 cut(s) 736
BsiHKCI CYCGRG 2 cut(s) 596, 813
BsiSI CCGG 2 cut(s) 297, 597
BsmAI GTCTC 3 cut(s) 91, 615, 708
BsmI GAATGC 2 cut(s) 424, 930
BsnI GGCC 5 cut(s) 88, 534, 564, 576, 735
Bso31I GGTCTC 1 cut(s) 615
BsoBI CYCGRG 2 cut(s) 596, 813
Bsp1286I GDGCHC 2 cut(s) 252, 843
Bsp143I GATC 7 cut(s) 9, 316, 403, 458, 475, 696, 985
BspACI CCGC 1 cut(s) 732
BspANI GGCC 5 cut(s) 88, 534, 564, 576, 735
BspCNI CTCAG 5 cut(s) 123, 316, 661, 906, 953
BspDI ATCGAT 2 cut(s) 44, 848
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 4 cut(s) 249, 649, 664, 842
BspMAI CTGCAG 1 cut(s) 257
BspPI GGATC 2 cut(s) 311, 466
BspT107I GGYRCC 3 cut(s) 247, 647, 662
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 615
BsrDI GCAATG 2 cut(s) 164, 959
BsrI ACTGG 1 cut(s) 249
BssECI CCNNGG 2 cut(s) 596, 835
BssMI GATC 7 cut(s) 9, 316, 403, 458, 475, 696, 985
BssNAI GTATAC 1 cut(s) 971
Bst1107I GTATAC 1 cut(s) 971
Bst2UI CCWGG 4 cut(s) 90, 486, 561, 836
Bst4CI ACNGT 2 cut(s) 304, 862
Bst6I CTCTTC 1 cut(s) 801
BstAFI CTTAAG 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 532, 751
BstDEI CTNAG 5 cut(s) 110, 324, 669, 893, 940
BstF5I GGATG 2 cut(s) 224, 528
BstHHI GCGC 1 cut(s) 446
BstKTI GATC 7 cut(s) 12, 319, 406, 461, 478, 699, 988
BstMAI GTCTC 3 cut(s) 91, 615, 708
BstMBI GATC 7 cut(s) 9, 316, 403, 458, 475, 696, 985
BstMCI CGRYCG 1 cut(s) 736
BstMWI GCNNNNNNNGC 2 cut(s) 582, 950
BstNI CCWGG 4 cut(s) 90, 486, 561, 836
BstNSI RCATGY 1 cut(s) 223
BstSCI CCNGG 6 cut(s) 88, 484, 559, 595, 596, 834
BstSFI CTRYAG 2 cut(s) 253, 465
BstSLI GKGCMC 1 cut(s) 252
BstV1I GCAGC 2 cut(s) 316, 843
BstV2I GAAGAC 1 cut(s) 297
BstZ17I GTATAC 1 cut(s) 971
BstZI CGGCCG 1 cut(s) 733
Bsu15I ATCGAT 2 cut(s) 44, 848
Bsu36I CCTNAGG 1 cut(s) 110
BsuI GTATCC 1 cut(s) 540
BsuRI GGCC 5 cut(s) 88, 534, 564, 576, 735
BsuTUI ATCGAT 2 cut(s) 44, 848
BtsCI GGATG 2 cut(s) 224, 528
BtsIMutI CAGTG 1 cut(s) 155
Cac8I GCNNGC 2 cut(s) 532, 751
CciI TCATGA 1 cut(s) 397
CfoI GCGC 1 cut(s) 446
Cfr9I CCCGGG 1 cut(s) 596
ClaI ATCGAT 2 cut(s) 44, 848
CseI GACGC 1 cut(s) 953
Csp6I GTAC 2 cut(s) 648, 663
CviAII CATG 4 cut(s) 151, 220, 398, 536
CviQI GTAC 2 cut(s) 648, 663
DdeI CTNAG 5 cut(s) 110, 324, 669, 893, 940
DpnI GATC 7 cut(s) 11, 318, 405, 460, 477, 698, 987
DpnII GATC 7 cut(s) 9, 316, 403, 458, 475, 696, 985
EaeI YGGCCR 3 cut(s) 532, 562, 733
EagI CGGCCG 1 cut(s) 733
Eam1104I CTCTTC 1 cut(s) 801
EarI CTCTTC 1 cut(s) 801
EclXI CGGCCG 1 cut(s) 733
Eco24I GRGCYC 1 cut(s) 843
Eco31I GGTCTC 1 cut(s) 615
Eco32I GATATC 1 cut(s) 913
Eco52I CGGCCG 1 cut(s) 733
Eco57I CTGAAG 3 cut(s) 524, 782, 975
Eco81I CCTNAGG 1 cut(s) 110
Eco88I CYCGRG 2 cut(s) 596, 813
EcoRII CCWGG 4 cut(s) 88, 484, 559, 834
EcoRV GATATC 1 cut(s) 913
EcoT38I GRGCYC 1 cut(s) 843
FaeI CATG 4 cut(s) 154, 223, 401, 539
FatI CATG 4 cut(s) 150, 219, 397, 535
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 1 cut(s) 970
Fnu4HI GCNGC 3 cut(s) 330, 733, 832
FokI GGATG 2 cut(s) 211, 535
FriOI GRGCYC 1 cut(s) 843
Fsp4HI GCNGC 3 cut(s) 330, 733, 832
FspBI CTAG 2 cut(s) 60, 177
GlaI GCGC 1 cut(s) 445
GluI GCNGC 3 cut(s) 330, 733, 832
HaeIII GGCC 5 cut(s) 88, 534, 564, 576, 735
HapII CCGG 2 cut(s) 297, 597
HgaI GACGC 1 cut(s) 953
HhaI GCGC 1 cut(s) 446
Hin1II CATG 4 cut(s) 154, 223, 401, 539
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HindIII AAGCTT 2 cut(s) 18, 118
HinfI GANTC 6 cut(s) 46, 309, 481, 816, 850, 938
HpaII CCGG 2 cut(s) 297, 597
HphI GGTGA 1 cut(s) 463
Hpy166II GTNNAC 1 cut(s) 971
Hpy188I TCNGA 8 cut(s) 325, 480, 504, 546, 658, 896, 917, 995
Hpy188III TCNNGA 3 cut(s) 112, 398, 935
Hpy8I GTNNAC 1 cut(s) 971
Hpy99I CGWCG 1 cut(s) 969
HpyAV CCTTC 4 cut(s) 60, 330, 499, 653
HpyCH4III ACNGT 2 cut(s) 304, 862
HpyCH4V TGCA 4 cut(s) 255, 283, 332, 809
HpyF10VI GCNNNNNNNGC 2 cut(s) 582, 950
HpyF3I CTNAG 5 cut(s) 110, 324, 669, 893, 940
Hsp92II CATG 4 cut(s) 154, 223, 401, 539
HspAI GCGC 1 cut(s) 444
KpnI GGTACC 2 cut(s) 651, 666
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 7 cut(s) 9, 316, 403, 458, 475, 696, 985
LmnI GCTCC 2 cut(s) 173, 846
Lsp1109I GCAGC 2 cut(s) 316, 843
LweI GCATC 1 cut(s) 666
MaeI CTAG 2 cut(s) 60, 177
MaeIII GTNAC 1 cut(s) 881
MalI GATC 7 cut(s) 11, 318, 405, 460, 477, 698, 987
MboI GATC 7 cut(s) 9, 316, 403, 458, 475, 696, 985
MboII GAAGA 4 cut(s) 302, 370, 788, 818
MhlI GDGCHC 2 cut(s) 252, 843
MlsI TGGCCA 2 cut(s) 534, 564
MluCI AATT 5 cut(s) 37, 361, 367, 425, 489
MluNI TGGCCA 2 cut(s) 534, 564
MlyI GAGTC 3 cut(s) 318, 825, 932
MmeI TCCRAC 3 cut(s) 240, 286, 878
MnlI CCTC 6 cut(s) 62, 119, 238, 319, 532, 902
Mox20I TGGCCA 2 cut(s) 534, 564
MroXI GAANNNNTTC 1 cut(s) 795
MscI TGGCCA 2 cut(s) 534, 564
MseI TTAA 4 cut(s) 189, 233, 371, 926
MslI CAYNNNNRTG 2 cut(s) 396, 399
Msp20I TGGCCA 2 cut(s) 534, 564
MspA1I CMGCKG 1 cut(s) 464
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 2 cut(s) 297, 597
MspR9I CCNGG 6 cut(s) 90, 486, 561, 597, 598, 836
Mva1269I GAATGC 2 cut(s) 424, 930
MvaI CCWGG 4 cut(s) 90, 486, 561, 836
MwoI GCNNNNNNNGC 2 cut(s) 582, 950
NciI CCSGG 2 cut(s) 597, 598
NdeII GATC 7 cut(s) 9, 316, 403, 458, 475, 696, 985
NlaIII CATG 4 cut(s) 154, 223, 401, 539
NlaIV GGNNCC 4 cut(s) 249, 649, 664, 842
NspI RCATGY 1 cut(s) 223
PaeR7I CTCGAG 1 cut(s) 813
PagI TCATGA 1 cut(s) 397
PcsI WCGNNNNNNNCGW 2 cut(s) 50, 854
PctI GAATGC 2 cut(s) 424, 930
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 3 cut(s) 46, 481, 850
PfoI TCCNGGA 1 cut(s) 484
PkrI GCNGC 3 cut(s) 331, 734, 833
PleI GAGTC 3 cut(s) 317, 824, 932
PpsI GAGTC 3 cut(s) 317, 824, 932
Psp6I CCWGG 4 cut(s) 88, 484, 559, 834
PspGI CCWGG 4 cut(s) 88, 484, 559, 834
PspN4I GGNNCC 4 cut(s) 249, 649, 664, 842
PspXI VCTCGAGB 1 cut(s) 813
PstI CTGCAG 1 cut(s) 257
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 2 cut(s) 649, 664
RsaNI GTAC 2 cut(s) 648, 663
RseI CAYNNNNRTG 2 cut(s) 396, 399
SaqAI TTAA 4 cut(s) 189, 233, 371, 926
SatI GCNGC 3 cut(s) 330, 733, 832
Sau3AI GATC 7 cut(s) 9, 316, 403, 458, 475, 696, 985
SchI GAGTC 3 cut(s) 318, 825, 932
ScrFI CCNGG 6 cut(s) 90, 486, 561, 597, 598, 836
SduI GDGCHC 2 cut(s) 252, 843
SfaNI GCATC 1 cut(s) 666
SfcI CTRYAG 2 cut(s) 253, 465
Sfr274I CTCGAG 1 cut(s) 813
SlaI CTCGAG 1 cut(s) 813
SmaI CCCGGG 1 cut(s) 598
SmiMI CAYNNNNRTG 2 cut(s) 396, 399
SmlI CTYRAG 3 cut(s) 188, 758, 813
SmoI CTYRAG 3 cut(s) 188, 758, 813
Sse9I AATT 5 cut(s) 37, 361, 367, 425, 489
SsiI CCGC 1 cut(s) 732
SspI AATATT 1 cut(s) 612
SspMI CTAG 2 cut(s) 60, 177
StyD4I CCNGG 6 cut(s) 88, 484, 559, 595, 596, 834
TaaI ACNGT 2 cut(s) 304, 862
TaqI TCGA 5 cut(s) 44, 240, 814, 848, 902
TasI AATT 5 cut(s) 37, 361, 367, 425, 489
TauI GCSGC 1 cut(s) 735
TfiI GAWTC 3 cut(s) 46, 481, 850
Tru1I TTAA 4 cut(s) 189, 233, 371, 926
Tru9I TTAA 4 cut(s) 189, 233, 371, 926
TscAI CASTG 1 cut(s) 162
TseI GCWGC 2 cut(s) 329, 831
TspDTI ATGAA 5 cut(s) 12, 30, 303, 672, 804
TspGWI ACGGA 2 cut(s) 144, 763
TspMI CCCGGG 1 cut(s) 596
TspRI CASTG 1 cut(s) 162
Vha464I CTTAAG 1 cut(s) 188
XapI RAATTY 1 cut(s) 367
XceI RCATGY 1 cut(s) 223
XhoI CTCGAG 1 cut(s) 813
XmaI CCCGGG 1 cut(s) 596
XmiI GTMKAC 1 cut(s) 970
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 2 cut(s) 60, 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.