Rmu_sc0012777.1_g000001

hydroxyacylglutathione hydrolase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0012777.1
OG0011321
Physical Location & Seq
Forward (+)
1485 .. 5730
4246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0012777.1_g000001.1.cds

Sequence Viewer

Length: 981 bp
atgcagacgacgatctctaaggcctcttccgccatggtctccctcccttgctctagggggaggagtgggctgtgtgtttggcctgggatgaggcagctttgctttaggaaaggcctcttgtatggattcatgcaacttttgtctgtgccgttgaaatccttgcgtggagcggcgagtcgatcgcttgaagtttctcagttctgcagtgtttccaaaatctcggcctcgttgcaaatcgaattggtaccgtgtcttagagataactacgcatatcttctgcacgatgtggatactggcacagttggtgttgtagatccttctgaagctgtacctgttatagatgctttgagtaagaaaaaccgaaatctgacatatatactgaatactcatcatcatcatgatcacactgggggaaatgcagagttaaaagcaaggtacggtgcaaaggtgattggttcaggaatagaccgagacagaattcctggcattgacatagttttgaatgacggagacaaatggatgtttgcaggccatgaggtccatgttatggggacgcctggtcacatcagcttctactttcctgggtcagggtcaatatttacaggagacactttgttcagcctatcatgtggcaagttgtttgaaggaacacctgaccagatgcagtcttcccttacaaagatcatgtctttgccagatgatacagaagtatactttggtcatgaatatacattgagtaactctaagtttgccttatctattgaacctgagaatgaagcacttcagtcctacgcagcccgtgtcgcacatcttcgcagcaaaggggtgcctacggttcctacaacattgttactggagaaggcttgcaatccttttcttcgcacatccagtacagagatccggcagtcgttaaatatttcagcctcagcaagtgaggcagaagcattgggtgtcatacgccaagcaaaggatactttctga

Protein Analysis

326

Amino Acids

35.46

Weight (kDa)

7.16

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011321)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06130 AT1G06130 AT2G31350 AT2G43430 AT2G43430
fragaria_vesca FvH4_3g41770 FvH4_3g41770
malus_domestica MD04G1045200.v1.1
prunus_persica Prupe.1G198300_v2.0.a1 Prupe.1G198300_v2.0.a1
pyrus_communis pycom04g03890
rosa_chinensis RchiOBHm_Chr5g0074861
rosa_laevigata RLG00000036511
rosa_multiflora Rmu_sc0003945.1_g000008 Rmu_sc0012777.1_g000001
rosa_roxburghii Rroxscaffold_1G00006720
rosa_rugosa Rorug05G0435000
rosa_samantha Rh5CG537400 Rh5DG525800
rosa_wichuraiana Rw5G045610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 244
AccB1I GGYRCC 2 cut(s) 244, 826
AccB7I CCANNNNNTGG 1 cut(s) 547
AccBSI CCGCTC 1 cut(s) 170
AccI GTMKAC 1 cut(s) 711
AciI CCGC 2 cut(s) 30, 170
AclWI GGATC 2 cut(s) 308, 892
AcsI RAATTY 1 cut(s) 477
AcuI CTGAAG 2 cut(s) 342, 767
AcyI GRCGYC 1 cut(s) 554
AdeI CACNNNGTG 1 cut(s) 286
AfaI GTAC 4 cut(s) 246, 330, 437, 892
AfiI CCNNNNNNNGG 3 cut(s) 547, 587, 967
AgsI TTSAA 5 cut(s) 154, 188, 502, 644, 764
AhdI GACNNNNNGTC 1 cut(s) 558
AjnI CCWGG 4 cut(s) 82, 481, 556, 580
AjuI GAANNNNNNNTTGG 2 cut(s) 699, 731
AluBI AGCT 3 cut(s) 97, 326, 570
AluI AGCT 3 cut(s) 97, 326, 570
Alw26I GTCTC 4 cut(s) 43, 465, 504, 600
AlwI GGATC 2 cut(s) 308, 892
AoxI GGCC 5 cut(s) 21, 80, 112, 222, 529
ApeKI GCWGC 3 cut(s) 94, 794, 816
ApoI RAATTY 1 cut(s) 477
ArsI GACNNNNNNTTYG 2 cut(s) 671, 703
Asp700I GAANNNNTTC 1 cut(s) 780
Asp718I GGTACC 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 538
AsuHPI GGTGA 1 cut(s) 460
AvaII GGWCC 1 cut(s) 538
BaeI ACNNNNGTAYC 2 cut(s) 693, 726
BanI GGYRCC 2 cut(s) 244, 826
BbsI GAAGAC 1 cut(s) 660
BbvCI CCTCAGC 1 cut(s) 925
BbvI GCAGC 3 cut(s) 106, 806, 828
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 4 cut(s) 84, 483, 558, 582
BciVI GTATCC 2 cut(s) 283, 964
BclI TGATCA 1 cut(s) 400
BcoDI GTCTC 4 cut(s) 43, 465, 504, 600
BfaI CTAG 1 cut(s) 54
BfmI CTRYAG 1 cut(s) 202
BfuI GTATCC 2 cut(s) 283, 964
BisI GCNGC 4 cut(s) 95, 171, 795, 817
BlsI GCNGC 4 cut(s) 96, 172, 796, 818
Bme1390I CCNGG 4 cut(s) 84, 483, 558, 582
Bme18I GGWCC 1 cut(s) 538
BmeRI GACNNNNNGTC 1 cut(s) 558
BmgT120I GGNCC 1 cut(s) 538
BmiI GGNNCC 3 cut(s) 246, 828, 837
BmrFI CCNGG 4 cut(s) 84, 483, 558, 582
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 2 cut(s) 331, 651
BmuI ACTGGG 1 cut(s) 417
BpiI GAAGAC 1 cut(s) 660
BpmI CTGGAG 1 cut(s) 875
Bpu10I CCTNAGC 1 cut(s) 925
BsaHI GRCGYC 1 cut(s) 554
BsaI GGTCTC 1 cut(s) 43
BsaJI CCNNGG 3 cut(s) 33, 83, 581
Bsc4I CCNNNNNNNGG 3 cut(s) 547, 587, 967
Bse1I ACTGG 4 cut(s) 298, 412, 858, 888
BseBI CCWGG 4 cut(s) 84, 483, 558, 582
BseDI CCNNGG 3 cut(s) 33, 83, 581
BseGI GGATG 3 cut(s) 93, 525, 884
BseLI CCNNNNNNNGG 3 cut(s) 547, 587, 967
BseMII CTCAG 3 cut(s) 209, 759, 939
BseNI ACTGG 4 cut(s) 298, 412, 858, 888
BseRI GAGGAG 1 cut(s) 76
BseXI GCAGC 3 cut(s) 106, 806, 828
BsgI GTGCAG 1 cut(s) 263
Bsh1285I CGRYCG 1 cut(s) 182
BshFI GGCC 5 cut(s) 23, 82, 114, 224, 531
BshNI GGYRCC 2 cut(s) 244, 826
BsiEI CGRYCG 1 cut(s) 182
BsiSI CCGG 1 cut(s) 901
BslFI GGGAC 1 cut(s) 565
BslI CCNNNNNNNGG 3 cut(s) 547, 587, 967
BsmAI GTCTC 4 cut(s) 43, 465, 504, 600
BsmFI GGGAC 1 cut(s) 565
BsnI GGCC 5 cut(s) 23, 82, 114, 224, 531
Bso31I GGTCTC 1 cut(s) 43
Bsp143I GATC 6 cut(s) 12, 179, 313, 400, 681, 897
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 2 cut(s) 30, 170
BspANI GGCC 5 cut(s) 23, 82, 114, 224, 531
BspCNI CTCAG 3 cut(s) 208, 760, 938
BspHI TCATGA 2 cut(s) 397, 721
BspLI GGNNCC 3 cut(s) 246, 828, 837
BspMAI CTGCAG 1 cut(s) 206
BspPI GGATC 2 cut(s) 308, 892
BspT107I GGYRCC 2 cut(s) 244, 826
BspTNI GGTCTC 1 cut(s) 43
BsrBI CCGCTC 1 cut(s) 170
BsrI ACTGG 4 cut(s) 298, 412, 858, 888
BssECI CCNNGG 3 cut(s) 33, 83, 581
BssMI GATC 6 cut(s) 12, 179, 313, 400, 681, 897
BssNAI GTATAC 1 cut(s) 712
BssNI GRCGYC 1 cut(s) 554
BssT1I CCWWGG 1 cut(s) 33
Bst1107I GTATAC 1 cut(s) 712
Bst2UI CCWGG 4 cut(s) 84, 483, 558, 582
Bst4CI ACNGT 4 cut(s) 249, 301, 440, 835
Bst6I CTCTTC 1 cut(s) 31
BstACI GRCGYC 1 cut(s) 554
BstC8I GCNNGC 2 cut(s) 529, 865
BstDEI CTNAG 6 cut(s) 18, 195, 254, 744, 768, 925
BstDSI CCRYGG 1 cut(s) 33
BstENI CCTNNNNNAGG 1 cut(s) 585
BstF5I GGATG 3 cut(s) 93, 525, 884
BstKTI GATC 6 cut(s) 15, 182, 316, 403, 684, 900
BstMAI GTCTC 4 cut(s) 43, 465, 504, 600
BstMBI GATC 6 cut(s) 12, 179, 313, 400, 681, 897
BstMCI CGRYCG 1 cut(s) 182
BstMWI GCNNNNNNNGC 3 cut(s) 29, 803, 935
BstNI CCWGG 4 cut(s) 84, 483, 558, 582
BstSCI CCNGG 4 cut(s) 82, 481, 556, 580
BstSFI CTRYAG 1 cut(s) 202
BstV1I GCAGC 3 cut(s) 106, 806, 828
BstV2I GAAGAC 1 cut(s) 660
BstX2I RGATCY 2 cut(s) 313, 897
BstYI RGATCY 2 cut(s) 313, 897
BstZ17I GTATAC 1 cut(s) 712
BsuI GTATCC 2 cut(s) 283, 964
BsuRI GGCC 5 cut(s) 23, 82, 114, 224, 531
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 3 cut(s) 93, 525, 884
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 2 cut(s) 211, 405
Cac8I GCNNGC 2 cut(s) 529, 865
CciI TCATGA 2 cut(s) 397, 721
Cfr13I GGNCC 1 cut(s) 538
CseI GACGC 1 cut(s) 562
Csp6I GTAC 4 cut(s) 245, 329, 436, 891
CviAII CATG 8 cut(s) 34, 130, 398, 533, 542, 627, 685, 722
CviQI GTAC 4 cut(s) 245, 329, 436, 891
DdeI CTNAG 6 cut(s) 18, 195, 254, 744, 768, 925
DpnI GATC 6 cut(s) 14, 181, 315, 402, 683, 899
DpnII GATC 6 cut(s) 12, 179, 313, 400, 681, 897
DraIII CACNNNGTG 1 cut(s) 286
DriI GACNNNNNGTC 1 cut(s) 558
Eam1104I CTCTTC 1 cut(s) 31
Eam1105I GACNNNNNGTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 31
EciI GGCGGA 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 33
Eco147I AGGCCT 2 cut(s) 23, 114
Eco31I GGTCTC 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 538
Eco57I CTGAAG 2 cut(s) 342, 767
EcoNI CCTNNNNNAGG 1 cut(s) 585
EcoRI GAATTC 1 cut(s) 477
EcoRII CCWGG 4 cut(s) 82, 481, 556, 580
EcoT14I CCWWGG 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 8 cut(s) 37, 133, 401, 536, 545, 630, 688, 725
FalI AAGNNNNNCTT 2 cut(s) 737, 769
FaqI GGGAC 1 cut(s) 565
FatI CATG 8 cut(s) 33, 129, 397, 532, 541, 626, 684, 721
FbaI TGATCA 1 cut(s) 400
FblI GTMKAC 1 cut(s) 711
Fnu4HI GCNGC 4 cut(s) 95, 171, 795, 817
FokI GGATG 3 cut(s) 100, 532, 871
Fsp4HI GCNGC 4 cut(s) 95, 171, 795, 817
FspBI CTAG 1 cut(s) 54
GluI GCNGC 4 cut(s) 95, 171, 795, 817
GsuI CTGGAG 1 cut(s) 875
HaeIII GGCC 5 cut(s) 23, 82, 114, 224, 531
HapII CCGG 1 cut(s) 901
HgaI GACGC 1 cut(s) 562
Hin1I GRCGYC 1 cut(s) 554
Hin1II CATG 8 cut(s) 37, 133, 401, 536, 545, 630, 688, 725
HinfI GANTC 2 cut(s) 126, 175
HpaII CCGG 1 cut(s) 901
HphI GGTGA 1 cut(s) 460
Hpy166II GTNNAC 1 cut(s) 712
Hpy188I TCNGA 3 cut(s) 322, 369, 980
Hpy188III TCNNGA 3 cut(s) 398, 459, 722
Hpy8I GTNNAC 1 cut(s) 712
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 3 cut(s) 327, 638, 853
HpyCH4III ACNGT 4 cut(s) 249, 301, 440, 835
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 803, 935
HpyF3I CTNAG 6 cut(s) 18, 195, 254, 744, 768, 925
Hsp92I GRCGYC 1 cut(s) 554
Hsp92II CATG 8 cut(s) 37, 133, 401, 536, 545, 630, 688, 725
KpnI GGTACC 1 cut(s) 248
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 6 cut(s) 12, 179, 313, 400, 681, 897
LmnI GCTCC 1 cut(s) 167
Lsp1109I GCAGC 3 cut(s) 106, 806, 828
LweI GCATC 2 cut(s) 331, 651
MaeI CTAG 1 cut(s) 54
MaeIII GTNAC 3 cut(s) 560, 737, 849
MalI GATC 6 cut(s) 14, 181, 315, 402, 683, 899
MbiI CCGCTC 1 cut(s) 170
MboI GATC 6 cut(s) 12, 179, 313, 400, 681, 897
MboII GAAGA 5 cut(s) 18, 266, 660, 803, 869
MflI RGATCY 2 cut(s) 313, 897
MluCI AATT 2 cut(s) 239, 477
MlyI GAGTC 1 cut(s) 184
MnlI CCTC 9 cut(s) 34, 53, 54, 84, 125, 235, 529, 928, 934
MroXI GAANNNNTTC 1 cut(s) 780
MseI TTAA 2 cut(s) 425, 911
MslI CAYNNNNRTG 1 cut(s) 396
MspI CCGG 1 cut(s) 901
MspR9I CCNGG 4 cut(s) 84, 483, 558, 582
MvaI CCWGG 4 cut(s) 84, 483, 558, 582
MwoI GCNNNNNNNGC 3 cut(s) 29, 803, 935
NcoI CCATGG 1 cut(s) 33
NdeII GATC 6 cut(s) 12, 179, 313, 400, 681, 897
NlaIII CATG 8 cut(s) 37, 133, 401, 536, 545, 630, 688, 725
NlaIV GGNNCC 3 cut(s) 246, 828, 837
NmeAIII GCCGAG 1 cut(s) 200
NmuCI GTSAC 1 cut(s) 560
PagI TCATGA 2 cut(s) 397, 721
PceI AGGCCT 2 cut(s) 23, 114
PdmI GAANNNNTTC 1 cut(s) 780
PfeI GAWTC 1 cut(s) 126
PflMI CCANNNNNTGG 1 cut(s) 547
PkrI GCNGC 4 cut(s) 96, 172, 796, 818
Ple19I CGATCG 1 cut(s) 182
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
Psp6I CCWGG 4 cut(s) 82, 481, 556, 580
PspGI CCWGG 4 cut(s) 82, 481, 556, 580
PspN4I GGNNCC 3 cut(s) 246, 828, 837
PspPI GGNCC 1 cut(s) 538
PstI CTGCAG 1 cut(s) 206
PsuI RGATCY 2 cut(s) 313, 897
PvuI CGATCG 1 cut(s) 182
RsaI GTAC 4 cut(s) 246, 330, 437, 892
RsaNI GTAC 4 cut(s) 245, 329, 436, 891
RseI CAYNNNNRTG 1 cut(s) 396
SaqAI TTAA 2 cut(s) 425, 911
SatI GCNGC 4 cut(s) 95, 171, 795, 817
Sau3AI GATC 6 cut(s) 12, 179, 313, 400, 681, 897
Sau96I GGNCC 1 cut(s) 538
SchI GAGTC 1 cut(s) 184
ScrFI CCNGG 4 cut(s) 84, 483, 558, 582
SetI ASST 9 cut(s) 99, 328, 334, 437, 450, 540, 572, 655, 769
SfaNI GCATC 2 cut(s) 331, 651
SfcI CTRYAG 1 cut(s) 202
SinI GGWCC 1 cut(s) 538
SmiMI CAYNNNNRTG 1 cut(s) 396
Sse9I AATT 2 cut(s) 239, 477
SseBI AGGCCT 2 cut(s) 23, 114
SsiI CCGC 2 cut(s) 30, 170
SspI AATATT 2 cut(s) 597, 916
SspMI CTAG 1 cut(s) 54
StuI AGGCCT 2 cut(s) 23, 114
StyD4I CCNGG 4 cut(s) 82, 481, 556, 580
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 4 cut(s) 249, 301, 440, 835
TaqI TCGA 2 cut(s) 178, 237
TaqII GACCGA 1 cut(s) 483
TasI AATT 2 cut(s) 239, 477
TatI WGTACW 1 cut(s) 890
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 2 cut(s) 425, 911
Tru9I TTAA 2 cut(s) 425, 911
TscAI CASTG 2 cut(s) 211, 412
TseFI GTSAC 1 cut(s) 560
TseI GCWGC 3 cut(s) 94, 794, 816
Tsp45I GTSAC 1 cut(s) 560
TspDTI ATGAA 3 cut(s) 118, 738, 789
TspGWI ACGGA 1 cut(s) 522
TspRI CASTG 2 cut(s) 211, 412
Van91I CCANNNNNTGG 1 cut(s) 547
VpaK11BI GGWCC 1 cut(s) 538
XagI CCTNNNNNAGG 1 cut(s) 585
XapI RAATTY 1 cut(s) 477
XmiI GTMKAC 1 cut(s) 711
XmnI GAANNNNTTC 1 cut(s) 780
XspI CTAG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.