Rw5G045610

hydroxyacylglutathione hydrolase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011321
Physical Location & Seq
Forward (+)
79612777 .. 79617855
5079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045610.1

Sequence Viewer

Length: 993 bp
ATGCAGACGACGATCTCTAAGGCCTCTTCCGCCATGGTCTCCCTCCCTTGCTCTAGGGGGAGGAGTGGGCTGTGTGTTTGGCCTGGGATGAGGCAGCTTTGCTTCAGGAAAGGCCTCTTGTATGGATTCATGCAACTTTTGTCTGTGCCGTTGAAATCCTTGCGTGGAGCGGCGAGTCGATCGCTTGAAGTTTCTCAGTTCTGCAGTGTTTCCAAAATCTCGGCCTCGTTGCAAATCGAATTGGTACCGTGTCTTAGAGATAACTACGCATATCTTCTGCACGATGTGGATACTGGCACAGTTGGTGTTGTAGATCCTTCTGAAGCTGTACCTGTTATAGATGCTTTGAGTAAGAAAAACCGAAATCTGACATATATACTGAATACTCATCATCATCATGATCACACTGGGGGAAATGCAGAGTTAAAAGCAAGGTACGGTGCAAAGGTGATTGGTTCAGGAATAGACCGAGACAGAATTCCTGGCATTGACATTGTTTTGAATGACGGAGACAAATGGATGTTTGCAGGCCATGAGGTCCATGTTATGGGGACGCCTGGTCATACCAAAGGTCACATCAGCTTCTACTTTCCTGGGTCAGGGTCAATATTTACAGGAGACACTTTGTTCAGCCTATCATGTGGCAAGTTGTTTGAAGGAACACCTGACCAGATGCAGTCTTCCCTTACGAAGATCATGTCTTTGCCAGATGATACAGAAGTATACTTTGGTCATGAATATACATTGAGTAACTCTAAGTTTGCCTTATCTATTGAACCTGAGAATGAAGCACTTCAGTCCTACGCAGCCCGTGTCGCACATCTTCGCAGCAAAGGGGTGCCTACGGTTCCTACAACATTGTTACTGGAGAAGGCTTGCAATCCTTTTCTTCGCACATCCAGTACAGAGATCCGGCAGTCGTTAAATATATCAGCCTCAGCAAGTGAGGCGGAAGCATTGGGTGTCATACGCCAAGCCAAGGATACTTTCTGA

Protein Analysis

330

Amino Acids

35.88

Weight (kDa)

7.67

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 89 - 245 7.7e-21 Metallo-beta-lactamase superfamily
HAGH_C PF16123 246 - 330 7.5e-26 Hydroxyacylglutathione hydrolase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011321)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06130 AT1G06130 AT2G31350 AT2G43430 AT2G43430
fragaria_vesca FvH4_3g41770 FvH4_3g41770
malus_domestica MD04G1045200.v1.1
prunus_persica Prupe.1G198300_v2.0.a1 Prupe.1G198300_v2.0.a1
pyrus_communis pycom04g03890
rosa_chinensis RchiOBHm_Chr5g0074861
rosa_laevigata RLG00000036511
rosa_multiflora Rmu_sc0003945.1_g000008 Rmu_sc0012777.1_g000001
rosa_roxburghii Rroxscaffold_1G00006720
rosa_rugosa Rorug05G0435000
rosa_samantha Rh5CG537400 Rh5DG525800
rosa_wichuraiana Rw5G045610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 244
AccB1I GGYRCC 2 cut(s) 244, 838
AccB7I CCANNNNNTGG 1 cut(s) 547
AccBSI CCGCTC 1 cut(s) 170
AccI GTMKAC 1 cut(s) 723
AciI CCGC 3 cut(s) 30, 170, 950
AclWI GGATC 2 cut(s) 308, 904
AcsI RAATTY 1 cut(s) 477
AcuI CTGAAG 3 cut(s) 88, 342, 779
AcyI GRCGYC 1 cut(s) 554
AdeI CACNNNGTG 1 cut(s) 286
AfaI GTAC 4 cut(s) 246, 330, 437, 904
AfiI CCNNNNNNNGG 3 cut(s) 547, 599, 979
AgsI TTSAA 5 cut(s) 154, 188, 502, 656, 776
AhdI GACNNNNNGTC 1 cut(s) 558
AjnI CCWGG 4 cut(s) 82, 481, 556, 592
AjuI GAANNNNNNNTTGG 3 cut(s) 711, 743, 971
AluBI AGCT 3 cut(s) 97, 326, 582
AluI AGCT 3 cut(s) 97, 326, 582
Alw26I GTCTC 4 cut(s) 43, 465, 504, 612
AlwI GGATC 2 cut(s) 308, 904
AoxI GGCC 5 cut(s) 21, 80, 112, 222, 529
ApeKI GCWGC 3 cut(s) 94, 806, 828
ApoI RAATTY 1 cut(s) 477
ArsI GACNNNNNNTTYG 2 cut(s) 683, 715
Asp700I GAANNNNTTC 1 cut(s) 792
Asp718I GGTACC 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 538
AsuHPI GGTGA 1 cut(s) 460
AvaII GGWCC 1 cut(s) 538
BaeI ACNNNNGTAYC 2 cut(s) 705, 738
BanI GGYRCC 2 cut(s) 244, 838
BbsI GAAGAC 1 cut(s) 672
BbvCI CCTCAGC 1 cut(s) 937
BbvI GCAGC 3 cut(s) 106, 818, 840
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 4 cut(s) 84, 483, 558, 594
BciVI GTATCC 2 cut(s) 283, 976
BclI TGATCA 1 cut(s) 400
BcoDI GTCTC 4 cut(s) 43, 465, 504, 612
BfaI CTAG 1 cut(s) 54
BfmI CTRYAG 1 cut(s) 202
BfuI GTATCC 2 cut(s) 283, 976
BisI GCNGC 4 cut(s) 95, 171, 807, 829
BlsI GCNGC 4 cut(s) 96, 172, 808, 830
Bme1390I CCNGG 4 cut(s) 84, 483, 558, 594
Bme18I GGWCC 1 cut(s) 538
BmeRI GACNNNNNGTC 1 cut(s) 558
BmgT120I GGNCC 1 cut(s) 538
BmiI GGNNCC 3 cut(s) 246, 840, 849
BmrFI CCNGG 4 cut(s) 84, 483, 558, 594
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 2 cut(s) 331, 663
BmuI ACTGGG 1 cut(s) 417
BpiI GAAGAC 1 cut(s) 672
BpmI CTGGAG 1 cut(s) 887
Bpu10I CCTNAGC 1 cut(s) 937
BsaHI GRCGYC 1 cut(s) 554
BsaI GGTCTC 1 cut(s) 43
BsaJI CCNNGG 4 cut(s) 33, 83, 593, 978
Bsc4I CCNNNNNNNGG 3 cut(s) 547, 599, 979
Bse1I ACTGG 4 cut(s) 298, 412, 870, 900
BseBI CCWGG 4 cut(s) 84, 483, 558, 594
BseDI CCNNGG 4 cut(s) 33, 83, 593, 978
BseGI GGATG 3 cut(s) 93, 525, 896
BseLI CCNNNNNNNGG 3 cut(s) 547, 599, 979
BseMII CTCAG 3 cut(s) 209, 771, 951
BseNI ACTGG 4 cut(s) 298, 412, 870, 900
BseRI GAGGAG 1 cut(s) 76
BseXI GCAGC 3 cut(s) 106, 818, 840
BsgI GTGCAG 1 cut(s) 263
Bsh1285I CGRYCG 1 cut(s) 182
BshFI GGCC 5 cut(s) 23, 82, 114, 224, 531
BshNI GGYRCC 2 cut(s) 244, 838
BsiEI CGRYCG 1 cut(s) 182
BsiSI CCGG 1 cut(s) 913
BslFI GGGAC 1 cut(s) 565
BslI CCNNNNNNNGG 3 cut(s) 547, 599, 979
BsmAI GTCTC 4 cut(s) 43, 465, 504, 612
BsmFI GGGAC 1 cut(s) 565
BsnI GGCC 5 cut(s) 23, 82, 114, 224, 531
Bso31I GGTCTC 1 cut(s) 43
Bsp143I GATC 6 cut(s) 12, 179, 313, 400, 693, 909
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 3 cut(s) 30, 170, 950
BspANI GGCC 5 cut(s) 23, 82, 114, 224, 531
BspCNI CTCAG 3 cut(s) 208, 772, 950
BspHI TCATGA 2 cut(s) 397, 733
BspLI GGNNCC 3 cut(s) 246, 840, 849
BspMAI CTGCAG 1 cut(s) 206
BspPI GGATC 2 cut(s) 308, 904
BspT107I GGYRCC 2 cut(s) 244, 838
BspTNI GGTCTC 1 cut(s) 43
BsrBI CCGCTC 1 cut(s) 170
BsrI ACTGG 4 cut(s) 298, 412, 870, 900
BssECI CCNNGG 4 cut(s) 33, 83, 593, 978
BssMI GATC 6 cut(s) 12, 179, 313, 400, 693, 909
BssNAI GTATAC 1 cut(s) 724
BssNI GRCGYC 1 cut(s) 554
BssT1I CCWWGG 2 cut(s) 33, 978
Bst1107I GTATAC 1 cut(s) 724
Bst2UI CCWGG 4 cut(s) 84, 483, 558, 594
Bst4CI ACNGT 4 cut(s) 249, 301, 440, 847
Bst6I CTCTTC 1 cut(s) 31
BstACI GRCGYC 1 cut(s) 554
BstC8I GCNNGC 2 cut(s) 529, 877
BstDEI CTNAG 6 cut(s) 18, 195, 254, 756, 780, 937
BstDSI CCRYGG 1 cut(s) 33
BstENI CCTNNNNNAGG 1 cut(s) 597
BstF5I GGATG 3 cut(s) 93, 525, 896
BstKTI GATC 6 cut(s) 15, 182, 316, 403, 696, 912
BstMAI GTCTC 4 cut(s) 43, 465, 504, 612
BstMBI GATC 6 cut(s) 12, 179, 313, 400, 693, 909
BstMCI CGRYCG 1 cut(s) 182
BstMWI GCNNNNNNNGC 3 cut(s) 29, 815, 947
BstNI CCWGG 4 cut(s) 84, 483, 558, 594
BstSCI CCNGG 4 cut(s) 82, 481, 556, 592
BstSFI CTRYAG 1 cut(s) 202
BstV1I GCAGC 3 cut(s) 106, 818, 840
BstV2I GAAGAC 1 cut(s) 672
BstX2I RGATCY 2 cut(s) 313, 909
BstYI RGATCY 2 cut(s) 313, 909
BstZ17I GTATAC 1 cut(s) 724
BsuI GTATCC 2 cut(s) 283, 976
BsuRI GGCC 5 cut(s) 23, 82, 114, 224, 531
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 3 cut(s) 93, 525, 896
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 2 cut(s) 211, 405
Cac8I GCNNGC 2 cut(s) 529, 877
CciI TCATGA 2 cut(s) 397, 733
Cfr13I GGNCC 1 cut(s) 538
CseI GACGC 1 cut(s) 562
Csp6I GTAC 4 cut(s) 245, 329, 436, 903
CviAII CATG 8 cut(s) 34, 130, 398, 533, 542, 639, 697, 734
CviQI GTAC 4 cut(s) 245, 329, 436, 903
DdeI CTNAG 6 cut(s) 18, 195, 254, 756, 780, 937
DpnI GATC 6 cut(s) 14, 181, 315, 402, 695, 911
DpnII GATC 6 cut(s) 12, 179, 313, 400, 693, 909
DraIII CACNNNGTG 1 cut(s) 286
DriI GACNNNNNGTC 1 cut(s) 558
Eam1104I CTCTTC 1 cut(s) 31
Eam1105I GACNNNNNGTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 31
EciI GGCGGA 2 cut(s) 19, 965
Eco130I CCWWGG 2 cut(s) 33, 978
Eco147I AGGCCT 2 cut(s) 23, 114
Eco31I GGTCTC 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 538
Eco57I CTGAAG 3 cut(s) 88, 342, 779
EcoNI CCTNNNNNAGG 1 cut(s) 597
EcoRI GAATTC 1 cut(s) 477
EcoRII CCWGG 4 cut(s) 82, 481, 556, 592
EcoT14I CCWWGG 2 cut(s) 33, 978
ErhI CCWWGG 2 cut(s) 33, 978
FaeI CATG 8 cut(s) 37, 133, 401, 536, 545, 642, 700, 737
FalI AAGNNNNNCTT 2 cut(s) 749, 781
FaqI GGGAC 1 cut(s) 565
FatI CATG 8 cut(s) 33, 129, 397, 532, 541, 638, 696, 733
FbaI TGATCA 1 cut(s) 400
FblI GTMKAC 1 cut(s) 723
Fnu4HI GCNGC 4 cut(s) 95, 171, 807, 829
FokI GGATG 3 cut(s) 100, 532, 883
Fsp4HI GCNGC 4 cut(s) 95, 171, 807, 829
FspBI CTAG 1 cut(s) 54
GluI GCNGC 4 cut(s) 95, 171, 807, 829
GsuI CTGGAG 1 cut(s) 887
HaeIII GGCC 5 cut(s) 23, 82, 114, 224, 531
HapII CCGG 1 cut(s) 913
HgaI GACGC 1 cut(s) 562
Hin1I GRCGYC 1 cut(s) 554
Hin1II CATG 8 cut(s) 37, 133, 401, 536, 545, 642, 700, 737
HinfI GANTC 2 cut(s) 126, 175
HpaII CCGG 1 cut(s) 913
HphI GGTGA 1 cut(s) 460
Hpy166II GTNNAC 1 cut(s) 724
Hpy188I TCNGA 3 cut(s) 322, 369, 992
Hpy188III TCNNGA 4 cut(s) 106, 398, 459, 734
Hpy8I GTNNAC 1 cut(s) 724
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 3 cut(s) 327, 650, 865
HpyCH4III ACNGT 4 cut(s) 249, 301, 440, 847
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 815, 947
HpyF3I CTNAG 6 cut(s) 18, 195, 254, 756, 780, 937
Hsp92I GRCGYC 1 cut(s) 554
Hsp92II CATG 8 cut(s) 37, 133, 401, 536, 545, 642, 700, 737
KpnI GGTACC 1 cut(s) 248
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 6 cut(s) 12, 179, 313, 400, 693, 909
LmnI GCTCC 1 cut(s) 167
Lsp1109I GCAGC 3 cut(s) 106, 818, 840
LweI GCATC 2 cut(s) 331, 663
MaeI CTAG 1 cut(s) 54
MaeIII GTNAC 3 cut(s) 572, 749, 861
MalI GATC 6 cut(s) 14, 181, 315, 402, 695, 911
MbiI CCGCTC 1 cut(s) 170
MboI GATC 6 cut(s) 12, 179, 313, 400, 693, 909
MboII GAAGA 6 cut(s) 18, 266, 672, 703, 815, 881
MflI RGATCY 2 cut(s) 313, 909
MluCI AATT 2 cut(s) 239, 477
MlyI GAGTC 1 cut(s) 184
MnlI CCTC 9 cut(s) 34, 53, 54, 84, 125, 235, 529, 940, 946
MroXI GAANNNNTTC 1 cut(s) 792
MseI TTAA 2 cut(s) 425, 923
MslI CAYNNNNRTG 1 cut(s) 396
MspI CCGG 1 cut(s) 913
MspR9I CCNGG 4 cut(s) 84, 483, 558, 594
MvaI CCWGG 4 cut(s) 84, 483, 558, 594
MwoI GCNNNNNNNGC 3 cut(s) 29, 815, 947
NcoI CCATGG 1 cut(s) 33
NdeII GATC 6 cut(s) 12, 179, 313, 400, 693, 909
NlaIII CATG 8 cut(s) 37, 133, 401, 536, 545, 642, 700, 737
NlaIV GGNNCC 3 cut(s) 246, 840, 849
NmeAIII GCCGAG 1 cut(s) 200
NmuCI GTSAC 1 cut(s) 572
PagI TCATGA 2 cut(s) 397, 733
PceI AGGCCT 2 cut(s) 23, 114
PdmI GAANNNNTTC 1 cut(s) 792
PfeI GAWTC 1 cut(s) 126
PflMI CCANNNNNTGG 1 cut(s) 547
PkrI GCNGC 4 cut(s) 96, 172, 808, 830
Ple19I CGATCG 1 cut(s) 182
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
Psp6I CCWGG 4 cut(s) 82, 481, 556, 592
PspGI CCWGG 4 cut(s) 82, 481, 556, 592
PspN4I GGNNCC 3 cut(s) 246, 840, 849
PspPI GGNCC 1 cut(s) 538
PstI CTGCAG 1 cut(s) 206
PsuI RGATCY 2 cut(s) 313, 909
PvuI CGATCG 1 cut(s) 182
RsaI GTAC 4 cut(s) 246, 330, 437, 904
RsaNI GTAC 4 cut(s) 245, 329, 436, 903
RseI CAYNNNNRTG 1 cut(s) 396
SaqAI TTAA 2 cut(s) 425, 923
SatI GCNGC 4 cut(s) 95, 171, 807, 829
Sau3AI GATC 6 cut(s) 12, 179, 313, 400, 693, 909
Sau96I GGNCC 1 cut(s) 538
SchI GAGTC 1 cut(s) 184
ScrFI CCNGG 4 cut(s) 84, 483, 558, 594
SfaNI GCATC 2 cut(s) 331, 663
SfcI CTRYAG 1 cut(s) 202
SinI GGWCC 1 cut(s) 538
SmiMI CAYNNNNRTG 1 cut(s) 396
Sse9I AATT 2 cut(s) 239, 477
SseBI AGGCCT 2 cut(s) 23, 114
SsiI CCGC 3 cut(s) 30, 170, 950
SspI AATATT 1 cut(s) 609
SspMI CTAG 1 cut(s) 54
StuI AGGCCT 2 cut(s) 23, 114
StyD4I CCNGG 4 cut(s) 82, 481, 556, 592
StyI CCWWGG 2 cut(s) 33, 978
TaaI ACNGT 4 cut(s) 249, 301, 440, 847
TaqI TCGA 2 cut(s) 178, 237
TaqII GACCGA 1 cut(s) 483
TasI AATT 2 cut(s) 239, 477
TatI WGTACW 1 cut(s) 902
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 2 cut(s) 425, 923
Tru9I TTAA 2 cut(s) 425, 923
TscAI CASTG 2 cut(s) 211, 412
TseFI GTSAC 1 cut(s) 572
TseI GCWGC 3 cut(s) 94, 806, 828
Tsp45I GTSAC 1 cut(s) 572
TspDTI ATGAA 3 cut(s) 118, 750, 801
TspGWI ACGGA 1 cut(s) 522
TspRI CASTG 2 cut(s) 211, 412
Van91I CCANNNNNTGG 1 cut(s) 547
VpaK11BI GGWCC 1 cut(s) 538
XagI CCTNNNNNAGG 1 cut(s) 597
XapI RAATTY 1 cut(s) 477
XmiI GTMKAC 1 cut(s) 723
XmnI GAANNNNTTC 1 cut(s) 792
XspI CTAG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.