AT2G31800

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008354
Physical Location & Seq
Reverse (-)
13520325 .. 13523918
3594 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G31800.1

Sequence Viewer

Length: 1431 bp
ATGGCGAATGTAGTGGGACAGCTAAAACGAGGAATCTCGAGACAATTCTCAACTGGATCGCTTCGTCGTACGCTTAGTCGTCAATTCACGCGTCAAGCTTCACATGATCCTCGCCGGAACAATATGCGATTTAGTTTTGGTCGACAATCGTCTTTAGATCCGATTCGTCGGAGTCCTGATGGTTCTAACGGTCCGCAGCTTGCTGTACCGGATAATCTCGACGCTACTATGCAGCTTCTATTTGTGGCTTGTAGAGGTGATGTTGAAGGTGTTCAGGATCTGCTTGATGAAGGTATTGATGTTAATAGTATCGATCTTGATGGTCGTACGGCTCTTCATATCGCTGCTTGTGAAGGTCATGTCGATGTTGTCAAGCTTCTTCTTACTAGGAAGGCTAATATTGATGCTCGTGATCGTTGGGGAAGCACGGCAGCTGCTGATGCTAAGTATTATGGTAACATGGATGTTTTCAACATTTTGAAAGCCCGTGGAGCTAAAGTTCCGAAAACCAAAAGGACACCCATGGTTGTGGCGAATCCTCGTGAAGTTCCTGAGTACGAGTTAAATCCGCAGGAACTTCAAGTTCGGAAAGCTGATGGTATCTCAAAGGGAATATATCAAGTGGCTAAATGGAATGGGACTAAGGTTTCTGTAAAGATACTTGATAAGGATCTCTACAAGGATTCTGACACTATAAATGCCTTCAAACACGAACTTACTTTATTCGAGAAGGTACGTCATCCTAATGTTGTGCAATTTGTTGGAGCTGTTACTCAAAATGTCCCCATGATGATTGTGTCCGAGTATCATCCTAAAGGGGACCTTGGGAGCTATCTTCAAAAAAAAGGTCGTCTTTCTCCAGCCAAAGTTCTAAGATTTGCCCTCGATATAGCCAGGGGAATGAATTATCTTCACGAGTGTAAACCAGAACCAGTAATCCACTGTGATCTAAAACCCAAAAATATTATGCTCGATAGTGGAGGACATCTGAAGGTGGCGGGATTTGGTTTGATAAGTTTTGCAAAGTTATCATCTGATAAATCAAAAATCCTTAATCACGGGGCCCATATAGATCCTTCAAATTACTGTATGGCACCTGAGGTTTACAAAGATGAAATATTTGACAGGAGTGTGGATTCTTACTCTTTTGGTGTCGTATTATATGAGATGATTGAAGGAGTACAACCTTTCCATCCTAAACCCCCAGAAGAGGCAGTGAAGCTAATGTGTTTAGAAGGAAGAAGACCTTCGTTTAAAGCCAAGTCCAAAAGTTGTCCCCAAGAGATGAGAGAATTGATTGAGGAATGCTGGGATACGGAAACTTTTGTTAGACCAACATTTTCTGAGATCATAGTTCGATTGGACAAGATCTTTGTACACTGCTCAAAACAGGGATGGTGGAAAGATACATTCAAGTTCCCTTGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Pkinase_Tyr

Protein Analysis

476

Amino Acids

53.74

Weight (kDa)

9.12

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 80 - 168 2.5e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 83 - 128 1.3e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 94 - 144 1.3e-08 Ankyrin repeats (many copies)
Ank PF00023 107 - 139 9e-11 Ankyrin repeat
PK_Tyr_Ser-Thr PF07714 201 - 454 5.2e-51 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 206 - 451 4.7e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1093
AccI GTMKAC 1 cut(s) 142
AccII CGCG 1 cut(s) 91
AciI CCGC 3 cut(s) 194, 569, 998
AclWI GGATC 6 cut(s) 64, 101, 152, 285, 678, 1067
AcuI CTGAAG 1 cut(s) 1010
AfaI GTAC 7 cut(s) 70, 207, 328, 557, 735, 1182, 1377
AfiI CCNNNNNNNGG 1 cut(s) 1210
AflIII ACRYGT 1 cut(s) 89
AgsI TTSAA 9 cut(s) 266, 472, 481, 581, 706, 839, 1080, 1175, 1414
AjnI CCWGG 1 cut(s) 893
AloI GAACNNNNNNTCC 4 cut(s) 483, 515, 921, 953
Alw26I GTCTC 1 cut(s) 34
AlwI GGATC 6 cut(s) 64, 101, 152, 285, 678, 1067
AlwNI CAGNNNCTG 2 cut(s) 280, 437
Ama87I CYCGRG 1 cut(s) 37
AoxI GGCC 1 cut(s) 1062
ApaI GGGCCC 1 cut(s) 1066
ApeKI GCWGC 5 cut(s) 196, 232, 344, 431, 434
ArsI GACNNNNNNTTYG 4 cut(s) 832, 864, 1355, 1387
Asp700I GAANNNNTTC 2 cut(s) 270, 1246
AspS9I GGNCC 4 cut(s) 191, 820, 1062, 1063
AsuHPI GGTGA 1 cut(s) 269
AvaI CYCGRG 1 cut(s) 37
AvaII GGWCC 2 cut(s) 191, 820
AxyI CCTNAGG 1 cut(s) 1098
BaeGI GKGCMC 1 cut(s) 1066
BanI GGYRCC 1 cut(s) 1093
BanII GRGCYC 1 cut(s) 1066
BauI CACGAG 3 cut(s) 408, 540, 914
BbsI GAAGAC 1 cut(s) 1249
BbvI GCAGC 5 cut(s) 208, 244, 331, 421, 443
BccI CCATC 5 cut(s) 173, 314, 590, 1200, 1389
BceAI ACGGC 2 cut(s) 345, 444
BciT130I CCWGG 1 cut(s) 895
BciVI GTATCC 1 cut(s) 1306
BcoDI GTCTC 1 cut(s) 34
BfaI CTAG 1 cut(s) 387
BfuI GTATCC 1 cut(s) 1306
BglII AGATCT 1 cut(s) 1368
BisI GCNGC 5 cut(s) 197, 233, 345, 432, 435
BlsI GCNGC 5 cut(s) 198, 234, 346, 433, 436
Bme1390I CCNGG 1 cut(s) 895
Bme18I GGWCC 2 cut(s) 191, 820
BmeT110I CYCGRG 1 cut(s) 37
BmgT120I GGNCC 4 cut(s) 191, 820, 1062, 1063
BmiI GGNNCC 4 cut(s) 821, 1063, 1064, 1095
BmrFI CCNGG 1 cut(s) 895
BmsI GCATC 2 cut(s) 394, 430
BoxI GACNNNNGTC 1 cut(s) 148
BpiI GAAGAC 1 cut(s) 1249
BpmI CTGGAG 1 cut(s) 843
Bsa29I ATCGAT 1 cut(s) 312
BsaBI GATNNNNATC 1 cut(s) 669
BsaJI CCNNGG 5 cut(s) 487, 522, 823, 894, 1421
BsaWI WCCGGW 1 cut(s) 208
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 1 cut(s) 1210
Bse1I ACTGG 2 cut(s) 58, 932
Bse21I CCTNAGG 1 cut(s) 1098
Bse8I GATNNNNATC 1 cut(s) 669
BseBI CCWGG 1 cut(s) 895
BseCI ATCGAT 1 cut(s) 312
BseDI CCNNGG 5 cut(s) 487, 522, 823, 894, 1421
BseGI GGATG 5 cut(s) 469, 739, 808, 1192, 1400
BseJI GATNNNNATC 1 cut(s) 669
BseLI CCNNNNNNNGG 1 cut(s) 1210
BseMII CTCAG 3 cut(s) 543, 1089, 1335
BseNI ACTGG 2 cut(s) 58, 932
BseSI GKGCMC 1 cut(s) 1066
BseXI GCAGC 5 cut(s) 208, 244, 331, 421, 443
BseYI CCCAGC 1 cut(s) 1308
Bsh1236I CGCG 1 cut(s) 91
BshFI GGCC 1 cut(s) 1064
BshNI GGYRCC 1 cut(s) 1093
BshVI ATCGAT 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 37
BsiSI CCGG 2 cut(s) 115, 209
BsiWI CGTACG 2 cut(s) 68, 326
BslFI GGGAC 5 cut(s) 30, 652, 767, 833, 1260
BslI CCNNNNNNNGG 1 cut(s) 1210
BsmAI GTCTC 1 cut(s) 34
BsmFI GGGAC 5 cut(s) 30, 652, 767, 833, 1260
BsmI GAATGC 1 cut(s) 1310
BsnI GGCC 1 cut(s) 1064
BsoBI CYCGRG 1 cut(s) 37
Bsp120I GGGCCC 1 cut(s) 1062
Bsp1286I GDGCHC 1 cut(s) 1066
Bsp1407I TGTACA 1 cut(s) 1375
Bsp19I CCATGG 1 cut(s) 522
BspACI CCGC 3 cut(s) 194, 569, 998
BspANI GGCC 1 cut(s) 1064
BspCNI CTCAG 3 cut(s) 544, 1090, 1336
BspDI ATCGAT 1 cut(s) 312
BspFNI CGCG 1 cut(s) 91
BspLI GGNNCC 4 cut(s) 821, 1063, 1064, 1095
BspPI GGATC 6 cut(s) 64, 101, 152, 285, 678, 1067
BspQI GCTCTTC 1 cut(s) 339
BspT107I GGYRCC 1 cut(s) 1093
BsrGI TGTACA 1 cut(s) 1375
BsrI ACTGG 2 cut(s) 58, 932
BssECI CCNNGG 5 cut(s) 487, 522, 823, 894, 1421
BssSI CACGAG 3 cut(s) 408, 540, 914
BssT1I CCWWGG 3 cut(s) 522, 823, 1421
Bst2BI CACGAG 3 cut(s) 408, 540, 914
Bst2UI CCWGG 1 cut(s) 895
Bst4CI ACNGT 3 cut(s) 191, 944, 1088
Bst6I CTCTTC 2 cut(s) 339, 1203
BstAUI TGTACA 1 cut(s) 1375
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 7 cut(s) 74, 444, 552, 642, 872, 1098, 1344
BstDSI CCRYGG 2 cut(s) 487, 522
BstF5I GGATG 5 cut(s) 469, 739, 808, 1192, 1400
BstFNI CGCG 1 cut(s) 91
BstMAI GTCTC 1 cut(s) 34
BstMWI GCNNNNNNNGC 2 cut(s) 440, 491
BstNI CCWGG 1 cut(s) 895
BstPAI GACNNNNGTC 1 cut(s) 148
BstSCI CCNGG 1 cut(s) 893
BstSLI GKGCMC 1 cut(s) 1066
BstUI CGCG 1 cut(s) 91
BstV1I GCAGC 5 cut(s) 208, 244, 331, 421, 443
BstV2I GAAGAC 1 cut(s) 1249
BstX2I RGATCY 5 cut(s) 157, 277, 670, 1072, 1368
BstXI CCANNNNNNTGG 1 cut(s) 529
BstYI RGATCY 5 cut(s) 157, 277, 670, 1072, 1368
Bsu15I ATCGAT 1 cut(s) 312
Bsu36I CCTNAGG 1 cut(s) 1098
BsuI GTATCC 1 cut(s) 1306
BsuRI GGCC 1 cut(s) 1064
BsuTUI ATCGAT 1 cut(s) 312
BtgI CCRYGG 2 cut(s) 487, 522
BtsCI GGATG 5 cut(s) 469, 739, 808, 1192, 1400
BtsI GCAGTG 2 cut(s) 1221, 1378
BtsIMutI CAGTG 3 cut(s) 940, 1221, 1378
Cac8I GCNNGC 1 cut(s) 201
CaiI CAGNNNCTG 2 cut(s) 280, 437
Cfr13I GGNCC 4 cut(s) 191, 820, 1062, 1063
ClaI ATCGAT 1 cut(s) 312
CpoI CGGWCCG 1 cut(s) 191
CseI GACGC 2 cut(s) 80, 230
Csp6I GTAC 7 cut(s) 69, 206, 327, 556, 734, 1181, 1376
CspI CGGWCCG 1 cut(s) 191
CviAII CATG 5 cut(s) 104, 359, 460, 523, 787
CviQI GTAC 7 cut(s) 69, 206, 327, 556, 734, 1181, 1376
DdeI CTNAG 7 cut(s) 74, 444, 552, 642, 872, 1098, 1344
DraI TTTAAA 1 cut(s) 1255
Eam1104I CTCTTC 2 cut(s) 339, 1203
EarI CTCTTC 2 cut(s) 339, 1203
Eco130I CCWWGG 3 cut(s) 522, 823, 1421
Eco24I GRGCYC 1 cut(s) 1066
Eco47I GGWCC 2 cut(s) 191, 820
Eco57I CTGAAG 1 cut(s) 1010
Eco81I CCTNAGG 1 cut(s) 1098
Eco88I CYCGRG 1 cut(s) 37
EcoO109I RGGNCCY 2 cut(s) 820, 1062
EcoRII CCWGG 1 cut(s) 893
EcoT14I CCWWGG 3 cut(s) 522, 823, 1421
EcoT38I GRGCYC 1 cut(s) 1066
ErhI CCWWGG 3 cut(s) 522, 823, 1421
FaeI CATG 5 cut(s) 107, 362, 463, 526, 790
FalI AAGNNNNNCTT 6 cut(s) 807, 839, 837, 869, 1231, 1263
FaqI GGGAC 5 cut(s) 30, 652, 767, 833, 1260
FatI CATG 5 cut(s) 103, 358, 459, 522, 786
FauI CCCGC 1 cut(s) 991
FblI GTMKAC 1 cut(s) 142
Fnu4HI GCNGC 5 cut(s) 197, 233, 345, 432, 435
FokI GGATG 5 cut(s) 476, 726, 795, 1179, 1407
FriOI GRGCYC 1 cut(s) 1066
Fsp4HI GCNGC 5 cut(s) 197, 233, 345, 432, 435
FspBI CTAG 1 cut(s) 387
GluI GCNGC 5 cut(s) 197, 233, 345, 432, 435
GsaI CCCAGC 1 cut(s) 1312
GsuI CTGGAG 1 cut(s) 843
HaeIII GGCC 1 cut(s) 1064
HapII CCGG 2 cut(s) 115, 209
HgaI GACGC 2 cut(s) 80, 230
Hin1II CATG 5 cut(s) 107, 362, 463, 526, 790
HincII GTYRAC 1 cut(s) 143
HindII GTYRAC 1 cut(s) 143
HindIII AAGCTT 2 cut(s) 96, 374
HinfI GANTC 6 cut(s) 33, 163, 172, 535, 683, 1136
HpaII CCGG 2 cut(s) 115, 209
HphI GGTGA 1 cut(s) 269
Hpy166II GTNNAC 4 cut(s) 143, 923, 1105, 1378
Hpy188I TCNGA 9 cut(s) 162, 171, 504, 588, 688, 802, 990, 1036, 1345
Hpy8I GTNNAC 4 cut(s) 143, 923, 1105, 1378
Hpy99I CGWCG 3 cut(s) 69, 171, 224
HpyCH4III ACNGT 3 cut(s) 191, 944, 1088
HpyCH4IV ACGT 1 cut(s) 736
HpyCH4V TGCA 3 cut(s) 232, 754, 1022
HpyF10VI GCNNNNNNNGC 2 cut(s) 440, 491
HpyF3I CTNAG 7 cut(s) 74, 444, 552, 642, 872, 1098, 1344
HpySE526I ACGT 1 cut(s) 736
Hsp92II CATG 5 cut(s) 107, 362, 463, 526, 790
LguI GCTCTTC 1 cut(s) 339
LmnI GCTCC 3 cut(s) 491, 764, 828
Lsp1109I GCAGC 5 cut(s) 208, 244, 331, 421, 443
LweI GCATC 2 cut(s) 394, 430
MaeI CTAG 1 cut(s) 387
MaeII ACGT 1 cut(s) 736
MaeIII GTNAC 2 cut(s) 455, 769
MboII GAAGA 7 cut(s) 326, 371, 827, 902, 1220, 1251, 1254
MflI RGATCY 5 cut(s) 157, 277, 670, 1072, 1368
MhlI GDGCHC 1 cut(s) 1066
MluCI AATT 6 cut(s) 44, 83, 755, 904, 1081, 1292
MluI ACGCGT 1 cut(s) 89
MlyI GAGTC 1 cut(s) 181
MmeI TCCRAC 2 cut(s) 149, 742
MnlI CCTC 9 cut(s) 23, 120, 248, 549, 893, 974, 1093, 1204, 1294
MroXI GAANNNNTTC 2 cut(s) 270, 1246
MseI TTAA 4 cut(s) 303, 563, 1053, 1254
MslI CAYNNNNRTG 3 cut(s) 363, 527, 744
MspA1I CMGCKG 1 cut(s) 434
MspI CCGG 2 cut(s) 115, 209
MspR9I CCNGG 1 cut(s) 895
Mva1269I GAATGC 1 cut(s) 1310
MvaI CCWGG 1 cut(s) 895
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 2 cut(s) 440, 491
NcoI CCATGG 1 cut(s) 522
NlaIII CATG 5 cut(s) 107, 362, 463, 526, 790
NlaIV GGNNCC 4 cut(s) 821, 1063, 1064, 1095
PaeR7I CTCGAG 1 cut(s) 37
PciSI GCTCTTC 1 cut(s) 339
PctI GAATGC 1 cut(s) 1310
PdmI GAANNNNTTC 2 cut(s) 270, 1246
PfeI GAWTC 5 cut(s) 33, 163, 535, 683, 1136
Pfl23II CGTACG 2 cut(s) 68, 326
PkrI GCNGC 5 cut(s) 198, 234, 346, 433, 436
PleI GAGTC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 180
PpuMI RGGWCCY 1 cut(s) 820
PshAI GACNNNNGTC 1 cut(s) 148
Psp5II RGGWCCY 1 cut(s) 820
Psp6I CCWGG 1 cut(s) 893
PspFI CCCAGC 1 cut(s) 1308
PspGI CCWGG 1 cut(s) 893
PspLI CGTACG 2 cut(s) 68, 326
PspN4I GGNNCC 4 cut(s) 821, 1063, 1064, 1095
PspOMI GGGCCC 1 cut(s) 1062
PspPI GGNCC 4 cut(s) 191, 820, 1062, 1063
PspPPI RGGWCCY 1 cut(s) 820
PstNI CAGNNNCTG 2 cut(s) 280, 437
PsuI RGATCY 5 cut(s) 157, 277, 670, 1072, 1368
PvuII CAGCTG 1 cut(s) 434
RsaI GTAC 7 cut(s) 70, 207, 328, 557, 735, 1182, 1377
RsaNI GTAC 7 cut(s) 69, 206, 327, 556, 734, 1181, 1376
RseI CAYNNNNRTG 3 cut(s) 363, 527, 744
Rsr2I CGGWCCG 1 cut(s) 191
RsrII CGGWCCG 1 cut(s) 191
SalI GTCGAC 1 cut(s) 141
SapI GCTCTTC 1 cut(s) 339
SaqAI TTAA 4 cut(s) 303, 563, 1053, 1254
SatI GCNGC 5 cut(s) 197, 233, 345, 432, 435
Sau96I GGNCC 4 cut(s) 191, 820, 1062, 1063
SchI GAGTC 1 cut(s) 181
ScrFI CCNGG 1 cut(s) 895
SduI GDGCHC 1 cut(s) 1066
SfaNI GCATC 2 cut(s) 394, 430
Sfr274I CTCGAG 1 cut(s) 37
SinI GGWCC 2 cut(s) 191, 820
SlaI CTCGAG 1 cut(s) 37
SmiMI CAYNNNNRTG 3 cut(s) 363, 527, 744
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
Sse9I AATT 6 cut(s) 44, 83, 755, 904, 1081, 1292
SsiI CCGC 3 cut(s) 194, 569, 998
SspI AATATT 3 cut(s) 400, 964, 1119
SspMI CTAG 1 cut(s) 387
StyD4I CCNGG 1 cut(s) 893
StyI CCWWGG 3 cut(s) 522, 823, 1421
TaaI ACNGT 3 cut(s) 191, 944, 1088
TaiI ACGT 1 cut(s) 739
TaqI TCGA 9 cut(s) 38, 142, 219, 312, 363, 726, 885, 972, 1357
TasI AATT 6 cut(s) 44, 83, 755, 904, 1081, 1292
TatI WGTACW 2 cut(s) 1180, 1375
TfiI GAWTC 5 cut(s) 33, 163, 535, 683, 1136
Tru1I TTAA 4 cut(s) 303, 563, 1053, 1254
Tru9I TTAA 4 cut(s) 303, 563, 1053, 1254
TscAI CASTG 3 cut(s) 947, 1221, 1385
TseI GCWGC 5 cut(s) 196, 232, 344, 431, 434
TspDTI ATGAA 4 cut(s) 303, 326, 917, 1128
TspGWI ACGGA 1 cut(s) 1331
TspRI CASTG 3 cut(s) 947, 1221, 1385
VpaK11BI GGWCC 2 cut(s) 191, 820
XhoI CTCGAG 1 cut(s) 37
XmiI GTMKAC 1 cut(s) 142
XmnI GAANNNNTTC 2 cut(s) 270, 1246
XspI CTAG 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.