Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0008354

Physical Location & Seq

Genomic Coordinates Forward (+)

227794 .. 232710

Gene Structure 4917 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1002900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1437 bp

ATGGAGAACATCGCGGCGCAGCTGAAGCGGGGGATATCGCGGCAGTTCTCCACGGGGTCGCTGAAGCGGACTCTCAGCCGCCAGTTCACCCGCCAATCCTCGCTCGACCCCAAGAGGAACAACTTGCGCTTCAGCTTCGGCAGGCAGTCCTCCCTCGACCCCATCCGCCGCAGCCCTTCCCACGACGATGACGACGAAGTCGATCTCTCCGTCCCCGAGAATCTCGACTCCACCATGAACCTCCTCTTCCTCGCCTGCCGCGGCGACGTCCACGGCGTCGAGGACGTCCTCAATCAAGGCATCGACGTCAACAGCATCGATTTGGACGGCCGTACGGCGCTCCATATCTCTGCCTGCGAGGGCCACGTCGAGGTCGTTAGGCTGTTGCTCTCCCGCAAGGCCAATATCGATGCTCGTGATCGCTGGGGCAGCACGGCAGCTGCAGATGCCAAGTATTACGGGAACACGGAAGTTTACAACATTCTGAAGGCACGTGGAGCCAAAGTTCCGAAAACCAGGAAGACACCAATGACTGTTGCGAATCCTCGAGAAGTTCCAGAGTATGAGCTCAATCCGTTAGAGCTTCAAGTTCGAAAGAGCGATGGTATCACAAAGGGATCGTATCAAGTGGCTAAATGGAATGGTACAAAGGTGTCTGTAAAGATACTTGACAAGGATTGCTACACGGACCCTGAAAGCATTAATGCCTTCAAACATGAGCTGGAGTTGTTAGAGAAGGTTCGGCATCCTAATGTCGTTCAATTTGTTGGTGCTGTCACCCAAAATATACCGATGATGATTGTGTCAGAGTATCATTCAAAAGGTGACTTGGGAAGCTATCTTCAAAAGAAAGGGCGCTTATCTCCATCAAAAGCCCTAAGATTTGCTCTTGACATTGCAAGGGGCATGAATTATCTTCATGAATGTAAACCAGACCCAATAATCCACTGTGATTTAAAGCCAAAAAATATTTTGCTCGATAATGGAGGTCAACTGAAAGTAGCTGGATTTGGCTTGATACGGTTGTCAAAAATCTCACCTAACAAAGCAAAACTAGCACAGCCTTGGGCTGACACTGACCTTTCAAATTTATATGTGGCACCTGAGATCTACAAAGATGAAATTTTCGACAGAAGTGTGGATGCATACTCTTTCGGTCTTATATTATATGAGATGATTGAGGGAATACAACCATTCCATCCCAAGCCTATTGAAGAATCTGTGAAGCTGATGTGTTTGGAAGGAAAAAGACCGCAGTTGAGGACCAAATCAAAAAGTTATCCCCCTGTACTGAAAGAGTTGATTGAGGAATGCTGGGATCCAGAACCTGTTATGAGACCCATTTTCTCTGAGATTATTGTACGCTTGGACAGGATAGTTGCGAATTGCTCAAAGCAGGGATGGTGGAAAGACACGTTTAAGCTTCCTTGGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009628 GO:0009898 GO:0009987 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0045087 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0098552 GO:0098562 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Pkinase_Tyr

Protein Analysis

479

Amino Acids

54.13

Weight (kDa)

8.87

Isoelectric Point (pI)

45.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	83 - 170	5.2e-12	Ankyrin repeats (3 copies)
Ank_5	PF13857	97 - 146	1.8e-06	Ankyrin repeats (many copies)
Ank	PF00023	109 - 141	7.4e-10	Ankyrin repeat
PK_Tyr_Ser-Thr	PF07714	203 - 455	3.1e-48	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	211 - 452	3.6e-42	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008354)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31800 AT2G43850 AT2G43850 AT2G43850 AT3G59830
fragaria_vesca	FvH4_1g00360 FvH4_1g00360
malus_domestica	MD02G1002900.v1.1 MD05G1054300.v1.1 MD15G1146100.v1.1
prunus_persica	Prupe.7G268300_v2.0.a1
pyrus_communis	pycom02g00150 pycom15g13120
rosa_chinensis	RchiOBHm_Chr2g0084791
rosa_laevigata	RLG00000015639
rosa_multiflora	Rmu_sc0002352.1_g000001 Rmu_ssc0000436.1_g000001
rosa_roxburghii	Rroxscaffold_2G00155810
rosa_rugosa	Rorug01G0456300
rosa_samantha	Rh2BG005000 Rh2CG005400 Rh2DG004800
rosa_wichuraiana	Rw2G000390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	197
AatII	GACGTC	3 cut(s)	270, 288, 309
AccB1I	GGYRCC	1 cut(s)	1099
AccII	CGCG	3 cut(s)	14, 40, 261
AclWI	GGATC	3 cut(s)	625, 1313, 1326
AcoI	YGGCCR	1 cut(s)	328
AcsI	RAATTY	2 cut(s)	1087, 1122
AcuI	CTGAAG	4 cut(s)	44, 83, 115, 506
AcvI	CACGTG	1 cut(s)	494
AcyI	GRCGYC	4 cut(s)	267, 276, 285, 306
AfaI	GTAC	4 cut(s)	334, 646, 1290, 1362
AfiI	CCNNNNNNNGG	1 cut(s)	370
AflIII	ACRYGT	1 cut(s)	1413
AgsI	TTSAA	7 cut(s)	587, 712, 761, 819, 845, 1086, 1214
AjiI	CACGTC	1 cut(s)	367
AjnI	CCWGG	1 cut(s)	515
AloI	GAACNNNNNNTCC	2 cut(s)	489, 521
Alw21I	GWGCWC	1 cut(s)	570
Alw26I	GTCTC	1 cut(s)	1330
AlwI	GGATC	3 cut(s)	625, 1313, 1326
AlwNI	CAGNNNCTG	1 cut(s)	1328
Ama87I	CYCGRG	2 cut(s)	215, 546
AoxI	GGCC	3 cut(s)	328, 361, 399
ApeKI	GCWGC	5 cut(s)	19, 171, 429, 437, 440
ApoI	RAATTY	2 cut(s)	1087, 1122
AseI	ATTAAT	1 cut(s)	702
AspLEI	GCGC	4 cut(s)	19, 129, 340, 858
AspS9I	GGNCC	3 cut(s)	361, 688, 1263
AsuHPI	GGTGA	4 cut(s)	79, 769, 836, 1029
AsuII	TTCGAA	1 cut(s)	592
AvaI	CYCGRG	2 cut(s)	215, 546
AvaII	GGWCC	2 cut(s)	688, 1263
BaeI	ACNNNNGTAYC	4 cut(s)	636, 669, 1010, 1043
BamHI	GGATCC	1 cut(s)	1318
BanI	GGYRCC	1 cut(s)	1099
BanII	GRGCYC	1 cut(s)	570
BauI	CACGAG	1 cut(s)	414
BbrPI	CACGTG	1 cut(s)	494
BbsI	GAAGAC	1 cut(s)	527
Bbv12I	GWGCWC	1 cut(s)	570
BbvI	GCAGC	5 cut(s)	31, 183, 427, 441, 449
BccI	CCATC	5 cut(s)	170, 596, 874, 1206, 1395
BceAI	ACGGC	5 cut(s)	289, 315, 343, 351, 450
BciT130I	CCWGG	1 cut(s)	517
BcoDI	GTCTC	1 cut(s)	1330
BfaI	CTAG	1 cut(s)	1055
BfmI	CTRYAG	1 cut(s)	441
BfoI	RGCGCY	2 cut(s)	341, 859
BglII	AGATCT	1 cut(s)	1107
Bme1390I	CCNGG	1 cut(s)	517
Bme18I	GGWCC	2 cut(s)	688, 1263
BmeT110I	CYCGRG	2 cut(s)	215, 546
BmgBI	CACGTC	1 cut(s)	367
BmgT120I	GGNCC	3 cut(s)	361, 688, 1263
BmiI	GGNNCC	4 cut(s)	499, 690, 1101, 1320
BmrFI	CCNGG	1 cut(s)	517
BmsI	GCATC	6 cut(s)	309, 324, 400, 436, 754, 1132
BpiI	GAAGAC	1 cut(s)	527
BpmI	CTGGAG	1 cut(s)	743
Bpu14I	TTCGAA	1 cut(s)	592
Bsa29I	ATCGAT	2 cut(s)	318, 408
BsaAI	YACGTR	1 cut(s)	494
BsaHI	GRCGYC	4 cut(s)	267, 276, 285, 306
BsaI	GGTCTC	1 cut(s)	1330
BsaJI	CCNNGG	5 cut(s)	51, 259, 271, 1064, 1427
BsaXI	ACNNNNNCTCC	2 cut(s)	489, 519
Bsc4I	CCNNNNNNNGG	1 cut(s)	370
Bse1I	ACTGG	1 cut(s)	82
Bse3DI	GCAATG	1 cut(s)	894
BseBI	CCWGG	1 cut(s)	517
BseCI	ATCGAT	2 cut(s)	318, 408
BseDI	CCNNGG	5 cut(s)	51, 259, 271, 1064, 1427
BseGI	GGATG	5 cut(s)	162, 745, 1147, 1198, 1406
BseLI	CCNNNNNNNGG	1 cut(s)	370
BseMI	GCAATG	1 cut(s)	894
BseMII	CTCAG	3 cut(s)	88, 1095, 1341
BseNI	ACTGG	1 cut(s)	82
BseRI	GAGGAG	1 cut(s)	233
BseX3I	CGGCCG	1 cut(s)	328
BseXI	GCAGC	5 cut(s)	31, 183, 427, 441, 449
BseYI	CCCAGC	2 cut(s)	423, 1314
Bsh1236I	CGCG	3 cut(s)	14, 40, 261
Bsh1285I	CGRYCG	1 cut(s)	331
BshFI	GGCC	3 cut(s)	330, 363, 401
BshNI	GGYRCC	1 cut(s)	1099
BshVI	ATCGAT	2 cut(s)	318, 408
BsiEI	CGRYCG	1 cut(s)	331
BsiHKAI	GWGCWC	1 cut(s)	570
BsiHKCI	CYCGRG	2 cut(s)	215, 546
BsiWI	CGTACG	1 cut(s)	332
BslFI	GGGAC	1 cut(s)	197
BslI	CCNNNNNNNGG	1 cut(s)	370
BsmAI	GTCTC	1 cut(s)	1330
BsmFI	GGGAC	1 cut(s)	197
BsmI	GAATGC	1 cut(s)	1316
BsnI	GGCC	3 cut(s)	330, 363, 401
Bso31I	GGTCTC	1 cut(s)	1330
BsoBI	CYCGRG	2 cut(s)	215, 546
Bsp119I	TTCGAA	1 cut(s)	592
Bsp1286I	GDGCHC	1 cut(s)	570
Bsp143I	GATC	5 cut(s)	202, 418, 617, 1107, 1318
BspANI	GGCC	3 cut(s)	330, 363, 401
BspCNI	CTCAG	3 cut(s)	87, 1096, 1342
BspDI	ATCGAT	2 cut(s)	318, 408
BspFNI	CGCG	3 cut(s)	14, 40, 261
BspHI	TCATGA	1 cut(s)	919
BspLI	GGNNCC	4 cut(s)	499, 690, 1101, 1320
BspMAI	CTGCAG	1 cut(s)	445
BspPI	GGATC	3 cut(s)	625, 1313, 1326
BspT104I	TTCGAA	1 cut(s)	592
BspT107I	GGYRCC	1 cut(s)	1099
BspTNI	GGTCTC	1 cut(s)	1330
BsrDI	GCAATG	1 cut(s)	894
BsrI	ACTGG	1 cut(s)	82
BssECI	CCNNGG	5 cut(s)	51, 259, 271, 1064, 1427
BssMI	GATC	5 cut(s)	202, 418, 617, 1107, 1318
BssNI	GRCGYC	4 cut(s)	267, 276, 285, 306
BssSI	CACGAG	1 cut(s)	414
BssT1I	CCWWGG	2 cut(s)	1064, 1427
Bst2BI	CACGAG	1 cut(s)	414
Bst2UI	CCWGG	1 cut(s)	517
Bst4CI	ACNGT	3 cut(s)	535, 950, 1023
Bst6I	CTCTTC	1 cut(s)	251
BstACI	GRCGYC	4 cut(s)	267, 276, 285, 306
BstBAI	YACGTR	1 cut(s)	494
BstBI	TTCGAA	1 cut(s)	592
BstC8I	GCNNGC	3 cut(s)	143, 256, 355
BstDEI	CTNAG	4 cut(s)	74, 878, 1104, 1350
BstDSI	CCRYGG	3 cut(s)	51, 259, 271
BstF5I	GGATG	5 cut(s)	162, 745, 1147, 1198, 1406
BstFNI	CGCG	3 cut(s)	14, 40, 261
BstH2I	RGCGCY	2 cut(s)	341, 859
BstHHI	GCGC	4 cut(s)	19, 129, 340, 858
BstKTI	GATC	5 cut(s)	205, 421, 620, 1110, 1321
BstMAI	GTCTC	1 cut(s)	1330
BstMBI	GATC	5 cut(s)	202, 418, 617, 1107, 1318
BstMCI	CGRYCG	1 cut(s)	331
BstMWI	GCNNNNNNNGC	5 cut(s)	25, 429, 446, 497, 1055
BstNI	CCWGG	1 cut(s)	517
BstSCI	CCNGG	1 cut(s)	515
BstSFI	CTRYAG	1 cut(s)	441
BstUI	CGCG	3 cut(s)	14, 40, 261
BstV1I	GCAGC	5 cut(s)	31, 183, 427, 441, 449
BstV2I	GAAGAC	1 cut(s)	527
BstX2I	RGATCY	2 cut(s)	1107, 1318
BstYI	RGATCY	2 cut(s)	1107, 1318
BstZI	CGGCCG	1 cut(s)	328
Bsu15I	ATCGAT	2 cut(s)	318, 408
BsuRI	GGCC	3 cut(s)	330, 363, 401
BsuTUI	ATCGAT	2 cut(s)	318, 408
BtgI	CCRYGG	3 cut(s)	51, 259, 271
BtgZI	GCGATG	1 cut(s)	615
BtrI	CACGTC	1 cut(s)	367
BtsCI	GGATG	5 cut(s)	162, 745, 1147, 1198, 1406
BtsIMutI	CAGTG	2 cut(s)	946, 1074
Cac8I	GCNNGC	3 cut(s)	143, 256, 355
CaiI	CAGNNNCTG	1 cut(s)	1328
CciI	TCATGA	1 cut(s)	919
CfoI	GCGC	4 cut(s)	19, 129, 340, 858
Cfr13I	GGNCC	3 cut(s)	361, 688, 1263
Cfr42I	CCGCGG	1 cut(s)	262
ClaI	ATCGAT	2 cut(s)	318, 408
CseI	GACGC	1 cut(s)	265
Csp6I	GTAC	4 cut(s)	333, 645, 1289, 1361
CviAII	CATG	4 cut(s)	235, 716, 907, 920
CviQI	GTAC	4 cut(s)	333, 645, 1289, 1361
DdeI	CTNAG	4 cut(s)	74, 878, 1104, 1350
DpnI	GATC	5 cut(s)	204, 420, 619, 1109, 1320
DpnII	GATC	5 cut(s)	202, 418, 617, 1107, 1318
DraI	TTTAAA	1 cut(s)	957
DrdI	GACNNNNNNGTC	1 cut(s)	197
DseDI	GACNNNNNNGTC	1 cut(s)	197
EaeI	YGGCCR	1 cut(s)	328
EagI	CGGCCG	1 cut(s)	328
Eam1104I	CTCTTC	1 cut(s)	251
EarI	CTCTTC	1 cut(s)	251
EciI	GGCGGA	1 cut(s)	155
Ecl136II	GAGCTC	1 cut(s)	568
EclXI	CGGCCG	1 cut(s)	328
Eco130I	CCWWGG	2 cut(s)	1064, 1427
Eco24I	GRGCYC	1 cut(s)	570
Eco31I	GGTCTC	1 cut(s)	1330
Eco32I	GATATC	1 cut(s)	36
Eco47I	GGWCC	2 cut(s)	688, 1263
Eco52I	CGGCCG	1 cut(s)	328
Eco53kI	GAGCTC	1 cut(s)	568
Eco57I	CTGAAG	4 cut(s)	44, 83, 115, 506
Eco72I	CACGTG	1 cut(s)	494
Eco88I	CYCGRG	2 cut(s)	215, 546
EcoICRI	GAGCTC	1 cut(s)	568
EcoRII	CCWGG	1 cut(s)	515
EcoRV	GATATC	1 cut(s)	36
EcoT14I	CCWWGG	2 cut(s)	1064, 1427
EcoT22I	ATGCAT	1 cut(s)	1147
EcoT38I	GRGCYC	1 cut(s)	570
ErhI	CCWWGG	2 cut(s)	1064, 1427
FaeI	CATG	4 cut(s)	238, 719, 910, 923
FaqI	GGGAC	1 cut(s)	197
FatI	CATG	4 cut(s)	234, 715, 906, 919
FauI	CCCGC	3 cut(s)	21, 98, 401
FokI	GGATG	5 cut(s)	149, 732, 1154, 1185, 1413
FriOI	GRGCYC	1 cut(s)	570
FspBI	CTAG	1 cut(s)	1055
GlaI	GCGC	4 cut(s)	18, 128, 339, 857
GsaI	CCCAGC	2 cut(s)	427, 1318
GsuI	CTGGAG	1 cut(s)	743
HaeII	RGCGCY	2 cut(s)	341, 859
HaeIII	GGCC	3 cut(s)	330, 363, 401
HgaI	GACGC	1 cut(s)	265
HhaI	GCGC	4 cut(s)	19, 129, 340, 858
Hin1I	GRCGYC	4 cut(s)	267, 276, 285, 306
Hin1II	CATG	4 cut(s)	238, 719, 910, 923
Hin6I	GCGC	4 cut(s)	17, 127, 338, 856
HinP1I	GCGC	4 cut(s)	17, 127, 338, 856
HincII	GTYRAC	2 cut(s)	310, 992
HindII	GTYRAC	2 cut(s)	310, 992
HindIII	AAGCTT	1 cut(s)	1421
HinfI	GANTC	5 cut(s)	70, 220, 227, 541, 1217
HphI	GGTGA	4 cut(s)	79, 769, 836, 1029
Hpy166II	GTNNAC	6 cut(s)	87, 271, 310, 475, 929, 992
Hpy188I	TCNGA	4 cut(s)	486, 510, 808, 1351
Hpy188III	TCNNGA	7 cut(s)	224, 416, 548, 557, 890, 920, 1322
Hpy8I	GTNNAC	6 cut(s)	87, 271, 310, 475, 929, 992
Hpy99I	CGWCG	6 cut(s)	188, 197, 269, 281, 308, 371
HpyAV	CCTTC	5 cut(s)	186, 481, 718, 730, 1235
HpyCH4III	ACNGT	3 cut(s)	535, 950, 1023
HpyCH4IV	ACGT	6 cut(s)	267, 285, 306, 366, 493, 1415
HpyCH4V	TGCA	3 cut(s)	443, 899, 1145
HpyF10VI	GCNNNNNNNGC	5 cut(s)	25, 429, 446, 497, 1055
HpyF3I	CTNAG	4 cut(s)	74, 878, 1104, 1350
HpySE526I	ACGT	6 cut(s)	267, 285, 306, 366, 493, 1415
Hsp92I	GRCGYC	4 cut(s)	267, 276, 285, 306
Hsp92II	CATG	4 cut(s)	238, 719, 910, 923
HspAI	GCGC	4 cut(s)	17, 127, 338, 856
KspI	CCGCGG	1 cut(s)	262
Kzo9I	GATC	5 cut(s)	202, 418, 617, 1107, 1318
LmnI	GCTCC	2 cut(s)	345, 497
Lsp1109I	GCAGC	5 cut(s)	31, 183, 427, 441, 449
LweI	GCATC	6 cut(s)	309, 324, 400, 436, 754, 1132
MaeI	CTAG	1 cut(s)	1055
MaeII	ACGT	6 cut(s)	267, 285, 306, 366, 493, 1415
MaeIII	GTNAC	2 cut(s)	775, 824
MalI	GATC	5 cut(s)	204, 420, 619, 1109, 1320
MboI	GATC	5 cut(s)	202, 418, 617, 1107, 1318
MboII	GAAGA	5 cut(s)	238, 532, 833, 908, 1226
MflI	RGATCY	2 cut(s)	1107, 1318
MhlI	GDGCHC	1 cut(s)	570
MluCI	AATT	5 cut(s)	761, 910, 1087, 1122, 1384
MlyI	GAGTC	2 cut(s)	64, 221
Mph1103I	ATGCAT	1 cut(s)	1147
MseI	TTAA	3 cut(s)	702, 956, 1419
MslI	CAYNNNNRTG	2 cut(s)	186, 750
MspA1I	CMGCKG	3 cut(s)	22, 261, 440
MspR9I	CCNGG	1 cut(s)	517
Mva1269I	GAATGC	1 cut(s)	1316
MvaI	CCWGG	1 cut(s)	517
MvnI	CGCG	3 cut(s)	14, 40, 261
MwoI	GCNNNNNNNGC	5 cut(s)	25, 429, 446, 497, 1055
NdeII	GATC	5 cut(s)	202, 418, 617, 1107, 1318
NlaIII	CATG	4 cut(s)	238, 719, 910, 923
NlaIV	GGNNCC	4 cut(s)	499, 690, 1101, 1320
NmuCI	GTSAC	2 cut(s)	775, 824
NsiI	ATGCAT	1 cut(s)	1147
NspV	TTCGAA	1 cut(s)	592
PaeR7I	CTCGAG	1 cut(s)	546
PagI	TCATGA	1 cut(s)	919
PcsI	WCGNNNNNNNCGW	6 cut(s)	189, 192, 198, 207, 273, 372
PctI	GAATGC	1 cut(s)	1316
PfeI	GAWTC	3 cut(s)	220, 541, 1217
Pfl23II	CGTACG	1 cut(s)	332
PflFI	GACNNNGTC	1 cut(s)	197
PleI	GAGTC	2 cut(s)	64, 221
PmaCI	CACGTG	1 cut(s)	494
PmlI	CACGTG	1 cut(s)	494
PpsI	GAGTC	2 cut(s)	64, 221
Ppu21I	YACGTR	1 cut(s)	494
PshBI	ATTAAT	1 cut(s)	702
Psp124BI	GAGCTC	1 cut(s)	570
Psp6I	CCWGG	1 cut(s)	515
PspCI	CACGTG	1 cut(s)	494
PspFI	CCCAGC	2 cut(s)	423, 1314
PspGI	CCWGG	1 cut(s)	515
PspLI	CGTACG	1 cut(s)	332
PspN4I	GGNNCC	4 cut(s)	499, 690, 1101, 1320
PspPI	GGNCC	3 cut(s)	361, 688, 1263
PstI	CTGCAG	1 cut(s)	445
PstNI	CAGNNNCTG	1 cut(s)	1328
PsuI	RGATCY	2 cut(s)	1107, 1318
PsyI	GACNNNGTC	1 cut(s)	197
PvuII	CAGCTG	2 cut(s)	22, 440
RsaI	GTAC	4 cut(s)	334, 646, 1290, 1362
RsaNI	GTAC	4 cut(s)	333, 645, 1289, 1361
RseI	CAYNNNNRTG	2 cut(s)	186, 750
SacI	GAGCTC	1 cut(s)	570
SacII	CCGCGG	1 cut(s)	262
SaqAI	TTAA	3 cut(s)	702, 956, 1419
Sau3AI	GATC	5 cut(s)	202, 418, 617, 1107, 1318
Sau96I	GGNCC	3 cut(s)	361, 688, 1263
SchI	GAGTC	2 cut(s)	64, 221
ScrFI	CCNGG	1 cut(s)	517
SduI	GDGCHC	1 cut(s)	570
SfaNI	GCATC	6 cut(s)	309, 324, 400, 436, 754, 1132
SfcI	CTRYAG	1 cut(s)	441
Sfr274I	CTCGAG	1 cut(s)	546
Sfr303I	CCGCGG	1 cut(s)	262
SfuI	TTCGAA	1 cut(s)	592
SgrBI	CCGCGG	1 cut(s)	262
SinI	GGWCC	2 cut(s)	688, 1263
SlaI	CTCGAG	1 cut(s)	546
SmiMI	CAYNNNNRTG	2 cut(s)	186, 750
SmlI	CTYRAG	1 cut(s)	546
SmoI	CTYRAG	1 cut(s)	546
Sse9I	AATT	5 cut(s)	761, 910, 1087, 1122, 1384
SspI	AATATT	1 cut(s)	970
SspMI	CTAG	1 cut(s)	1055
SstI	GAGCTC	1 cut(s)	570
StyD4I	CCNGG	1 cut(s)	515
StyI	CCWWGG	2 cut(s)	1064, 1427
TaaI	ACNGT	3 cut(s)	535, 950, 1023
TaiI	ACGT	6 cut(s)	270, 288, 309, 369, 496, 1418
TaqII	GACCGA	1 cut(s)	1145
TasI	AATT	5 cut(s)	761, 910, 1087, 1122, 1384
TatI	WGTACW	1 cut(s)	1288
TauI	GCSGC	6 cut(s)	17, 43, 81, 171, 261, 264
TfiI	GAWTC	3 cut(s)	220, 541, 1217
Tru1I	TTAA	3 cut(s)	702, 956, 1419
Tru9I	TTAA	3 cut(s)	702, 956, 1419
TscAI	CASTG	2 cut(s)	953, 1081
TseFI	GTSAC	2 cut(s)	775, 824
TseI	GCWGC	5 cut(s)	19, 171, 429, 437, 440
Tsp45I	GTSAC	2 cut(s)	775, 824
TspDTI	ATGAA	5 cut(s)	251, 908, 923, 936, 1134
TspGWI	ACGGA	4 cut(s)	199, 482, 564, 701
TspRI	CASTG	2 cut(s)	953, 1081
Tth111I	GACNNNGTC	1 cut(s)	197
VpaK11BI	GGWCC	2 cut(s)	688, 1263
VspI	ATTAAT	1 cut(s)	702
XapI	RAATTY	2 cut(s)	1087, 1122
XhoI	CTCGAG	1 cut(s)	546
XspI	CTAG	1 cut(s)	1055
ZraI	GACGTC	3 cut(s)	268, 286, 307
Zsp2I	ATGCAT	1 cut(s)	1147

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.