Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008354

Physical Location & Seq

Genomic Coordinates Forward (+)

10871178 .. 10875219

Gene Structure 4042 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1146100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1437 bp

ATGGAGAACATCGCGGCGCAGCTGAAGCGGGGGATATCACGGCAGTTCTCGACGGGGTCGCTGCGCCGGAACCTCAGTCGCCAGTTCTCCCGCCAATCCTCGCTCGACCCCAGGAGGAACAACTTGCGCTTCAGCTTCGGCAGGCAGTCCTCCCTCGACCCCATCCGCCGCAGCCCTTCCCACGACGATGACGACGACGTTGATCTCTCCGTCCCCGAGAATCTCGACTCCACCATGAACCTCCTTTTCCTCGCCTGCCGCGGCGACGTCCACGGCGTCGAGGACCTCCTCAATCAAGGCGTCGACGTCAACAGCATCGATTTGGACGGCCGTACGGCGCTCCATATCGCTGCCTGCGAGGGCCACGTCGAGGTTGCTAGGCTGTTGCTCTCCCGCAAGGCCAATATCGATGCTCGGGATCGCTGGGGCAGCACGGCAGCTGTAGATGCTAAGTATTACGGGAACACGGAAGTTTACAATATTCTGAAATCCCGTGGAGCCAAAGTTCCGAAAATCAGGAAGACACCAATGACTGTTGCGAATCCTCGAGAAGTTCCAGAGTATGAGCTCAATCCGTTAGAGCTTCACGTTCGAAAGAGTGATGGTATCACAAAGGGATCGTATCAAGTGGCTAAATGGAATGGTACGAAGGTTTCTGTAAAGATACTTGACAAGGATTGCTATACAGACCCTGAAAGCATTAATGCTTTCAAACATGAGCTGGAGTTGTTAGAGAAGGTTCGGCATCCTAATGTCGTTCAATTTGTTGGTGCCGTTACCCAAAATATACCGATGATGATTGTTTCAGAGTATCATTCGAAAGGTGACTTGGGGAGCTATCTTCAAAAGAAAGGGCGCTTATCTCCATCAAAAGCCCTAAGATTTGCTCTTGACATTGCAAGGGGCGTGAATTATCTTCATGAATGTAAACCAGACCCAGTAATCCACTGTGATTTAAAGCCAAAAAATATTTTGCTCGATAATGGAGGTCAACTGAAAGTAGCTGGATTTGGCTTGTTAAGATTGTCAAAAATCTCACCTGACAAAGCAAAACTAGCACAGCCTTGGGCTGACACTGACCTTTCAAGTTTATATGTGGCACCTGAGATCTACAAAGATGAAATTTTCACCAAAAGTGTGGATGTGTACTCTTTCGGTCTTATATTATATGAGATGGTTGAGGGATTACAACCATTCCATCCCAAGCCTATGGAAGAGGCTGCGAAACTGATGTGTTTGGAAGGAAAAAGACCGCAGCTGAGGACCAAATCAAAGGGTTATCCCCCTGTAGTAAAAGAGTTGATTGAGGAATGTTGGAATCCAGAACCTGTTATGAGGCCCATCTTCTCGGAGATTATTATACGGTTGGACAAGATAGTTTCGAATTGCTCAAAGCAGGGCTGGTGGAAGGACACGTTTAAGCTTCCTTGGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009628 GO:0009898 GO:0009987 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0045087 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0098552 GO:0098562 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Pkinase_Tyr

Protein Analysis

479

Amino Acids

54.02

Weight (kDa)

8.94

Isoelectric Point (pI)

44.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	83 - 169	1.3e-13	Ankyrin repeats (3 copies)
Ank_4	PF13637	87 - 130	1.4e-07	Ankyrin repeats (many copies)
Ank_5	PF13857	96 - 146	1.6e-07	Ankyrin repeats (many copies)
Ank	PF00023	109 - 141	1.6e-10	Ankyrin repeat
PK_Tyr_Ser-Thr	PF07714	203 - 455	3.1e-49	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	211 - 452	5e-43	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008354)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31800 AT2G43850 AT2G43850 AT2G43850 AT3G59830
fragaria_vesca	FvH4_1g00360 FvH4_1g00360
malus_domestica	MD02G1002900.v1.1 MD05G1054300.v1.1 MD15G1146100.v1.1
prunus_persica	Prupe.7G268300_v2.0.a1
pyrus_communis	pycom02g00150 pycom15g13120
rosa_chinensis	RchiOBHm_Chr2g0084791
rosa_laevigata	RLG00000015639
rosa_multiflora	Rmu_sc0002352.1_g000001 Rmu_ssc0000436.1_g000001
rosa_roxburghii	Rroxscaffold_2G00155810
rosa_rugosa	Rorug01G0456300
rosa_samantha	Rh2BG005000 Rh2CG005400 Rh2DG004800
rosa_wichuraiana	Rw2G000390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	270, 309
AccB1I	GGYRCC	2 cut(s)	770, 1099
AccI	GTMKAC	1 cut(s)	303
AccII	CGCG	2 cut(s)	14, 261
AciI	CCGC	9 cut(s)	14, 28, 91, 166, 169, 259, 261, 394, 1253
AclWI	GGATC	2 cut(s)	426, 625
AcoI	YGGCCR	1 cut(s)	328
AcsI	RAATTY	1 cut(s)	1122
AcuI	CTGAAG	2 cut(s)	44, 115
AcyI	GRCGYC	4 cut(s)	267, 276, 300, 306
AfaI	GTAC	3 cut(s)	334, 646, 1148
AfiI	CCNNNNNNNGG	1 cut(s)	370
AflIII	ACRYGT	1 cut(s)	1413
AgsI	TTSAA	4 cut(s)	712, 761, 845, 1086
AjiI	CACGTC	1 cut(s)	367
AjnI	CCWGG	1 cut(s)	110
AjuI	GAANNNNNNNTTGG	2 cut(s)	812, 844
AloI	GAACNNNNNNTCC	2 cut(s)	489, 521
Alw21I	GWGCWC	1 cut(s)	570
AlwI	GGATC	2 cut(s)	426, 625
AlwNI	CAGNNNCTG	2 cut(s)	692, 1328
Ama87I	CYCGRG	3 cut(s)	215, 414, 546
AoxI	GGCC	4 cut(s)	328, 361, 399, 1337
ApeKI	GCWGC	8 cut(s)	19, 61, 171, 350, 429, 437, 1220, 1255
ApoI	RAATTY	1 cut(s)	1122
AseI	ATTAAT	1 cut(s)	702
AspLEI	GCGC	5 cut(s)	19, 66, 129, 340, 858
AspS9I	GGNCC	4 cut(s)	283, 361, 1263, 1338
AsuHPI	GGTGA	3 cut(s)	836, 1029, 1120
AsuII	TTCGAA	3 cut(s)	592, 818, 1382
AvaI	CYCGRG	3 cut(s)	215, 414, 546
AvaII	GGWCC	2 cut(s)	283, 1263
BaeI	ACNNNNGTAYC	2 cut(s)	636, 669
BanI	GGYRCC	2 cut(s)	770, 1099
BanII	GRGCYC	1 cut(s)	570
BbsI	GAAGAC	1 cut(s)	527
Bbv12I	GWGCWC	1 cut(s)	570
BbvCI	CCTCAGC	1 cut(s)	1259
BbvI	GCAGC	8 cut(s)	31, 48, 183, 337, 441, 449, 1207, 1267
BccI	CCATC	6 cut(s)	170, 596, 874, 1168, 1206, 1349
BceAI	ACGGC	7 cut(s)	56, 289, 315, 343, 351, 450, 758
BciT130I	CCWGG	1 cut(s)	112
BfaI	CTAG	2 cut(s)	378, 1055
BfmI	CTRYAG	2 cut(s)	441, 1287
BfoI	RGCGCY	2 cut(s)	341, 859
BglII	AGATCT	1 cut(s)	1107
Bme1390I	CCNGG	1 cut(s)	112
Bme18I	GGWCC	2 cut(s)	283, 1263
BmeT110I	CYCGRG	3 cut(s)	215, 414, 546
BmgBI	CACGTC	1 cut(s)	367
BmgT120I	GGNCC	4 cut(s)	283, 361, 1263, 1338
BmiI	GGNNCC	4 cut(s)	71, 499, 772, 1101
BmrFI	CCNGG	1 cut(s)	112
BmrI	ACTGGG	1 cut(s)	932
BmsI	GCATC	4 cut(s)	324, 400, 436, 754
BmuI	ACTGGG	1 cut(s)	932
BpiI	GAAGAC	1 cut(s)	527
BpmI	CTGGAG	1 cut(s)	743
Bpu10I	CCTNAGC	1 cut(s)	1259
Bpu14I	TTCGAA	3 cut(s)	592, 818, 1382
Bsa29I	ATCGAT	2 cut(s)	318, 408
BsaHI	GRCGYC	4 cut(s)	267, 276, 300, 306
BsaJI	CCNNGG	6 cut(s)	110, 259, 271, 493, 1064, 1427
BsaXI	ACNNNNNCTCC	2 cut(s)	489, 519
Bsc4I	CCNNNNNNNGG	1 cut(s)	370
Bse1I	ACTGG	2 cut(s)	82, 938
Bse3DI	GCAATG	1 cut(s)	894
BseBI	CCWGG	1 cut(s)	112
BseCI	ATCGAT	2 cut(s)	318, 408
BseDI	CCNNGG	6 cut(s)	110, 259, 271, 493, 1064, 1427
BseGI	GGATG	4 cut(s)	162, 745, 1147, 1198
BseLI	CCNNNNNNNGG	1 cut(s)	370
BseMI	GCAATG	1 cut(s)	894
BseMII	CTCAG	3 cut(s)	88, 1095, 1250
BseNI	ACTGG	2 cut(s)	82, 938
BseRI	GAGGAG	1 cut(s)	278
BseX3I	CGGCCG	1 cut(s)	328
BseXI	GCAGC	8 cut(s)	31, 48, 183, 337, 441, 449, 1207, 1267
BseYI	CCCAGC	1 cut(s)	423
Bsh1236I	CGCG	2 cut(s)	14, 261
Bsh1285I	CGRYCG	1 cut(s)	331
BshFI	GGCC	4 cut(s)	330, 363, 401, 1339
BshNI	GGYRCC	2 cut(s)	770, 1099
BshVI	ATCGAT	2 cut(s)	318, 408
BsiEI	CGRYCG	1 cut(s)	331
BsiHKAI	GWGCWC	1 cut(s)	570
BsiHKCI	CYCGRG	3 cut(s)	215, 414, 546
BsiSI	CCGG	1 cut(s)	67
BsiWI	CGTACG	1 cut(s)	332
BslFI	GGGAC	1 cut(s)	197
BslI	CCNNNNNNNGG	1 cut(s)	370
BsmFI	GGGAC	1 cut(s)	197
BsnI	GGCC	4 cut(s)	330, 363, 401, 1339
BsoBI	CYCGRG	3 cut(s)	215, 414, 546
Bsp119I	TTCGAA	3 cut(s)	592, 818, 1382
Bsp1286I	GDGCHC	1 cut(s)	570
Bsp143I	GATC	4 cut(s)	202, 418, 617, 1107
BspACI	CCGC	9 cut(s)	14, 28, 91, 166, 169, 259, 261, 394, 1253
BspANI	GGCC	4 cut(s)	330, 363, 401, 1339
BspCNI	CTCAG	3 cut(s)	87, 1096, 1251
BspDI	ATCGAT	2 cut(s)	318, 408
BspFNI	CGCG	2 cut(s)	14, 261
BspHI	TCATGA	1 cut(s)	919
BspLI	GGNNCC	4 cut(s)	71, 499, 772, 1101
BspPI	GGATC	2 cut(s)	426, 625
BspT104I	TTCGAA	3 cut(s)	592, 818, 1382
BspT107I	GGYRCC	2 cut(s)	770, 1099
BsrDI	GCAATG	1 cut(s)	894
BsrI	ACTGG	2 cut(s)	82, 938
BssECI	CCNNGG	6 cut(s)	110, 259, 271, 493, 1064, 1427
BssMI	GATC	4 cut(s)	202, 418, 617, 1107
BssNI	GRCGYC	4 cut(s)	267, 276, 300, 306
BssT1I	CCWWGG	2 cut(s)	1064, 1427
Bst2UI	CCWGG	1 cut(s)	112
Bst4CI	ACNGT	3 cut(s)	535, 950, 1365
Bst6I	CTCTTC	1 cut(s)	1209
BstACI	GRCGYC	4 cut(s)	267, 276, 300, 306
BstBI	TTCGAA	3 cut(s)	592, 818, 1382
BstC8I	GCNNGC	3 cut(s)	143, 256, 355
BstDEI	CTNAG	5 cut(s)	74, 450, 878, 1104, 1259
BstDSI	CCRYGG	3 cut(s)	259, 271, 493
BstF5I	GGATG	4 cut(s)	162, 745, 1147, 1198
BstFNI	CGCG	2 cut(s)	14, 261
BstH2I	RGCGCY	2 cut(s)	341, 859
BstHHI	GCGC	5 cut(s)	19, 66, 129, 340, 858
BstKTI	GATC	4 cut(s)	205, 421, 620, 1110
BstMBI	GATC	4 cut(s)	202, 418, 617, 1107
BstMCI	CGRYCG	1 cut(s)	331
BstMWI	GCNNNNNNNGC	4 cut(s)	25, 429, 446, 1055
BstNI	CCWGG	1 cut(s)	112
BstSCI	CCNGG	1 cut(s)	110
BstSFI	CTRYAG	2 cut(s)	441, 1287
BstUI	CGCG	2 cut(s)	14, 261
BstV1I	GCAGC	8 cut(s)	31, 48, 183, 337, 441, 449, 1207, 1267
BstV2I	GAAGAC	1 cut(s)	527
BstX2I	RGATCY	1 cut(s)	1107
BstXI	CCANNNNNNTGG	2 cut(s)	1138, 1210
BstYI	RGATCY	1 cut(s)	1107
BstZI	CGGCCG	1 cut(s)	328
Bsu15I	ATCGAT	2 cut(s)	318, 408
BsuRI	GGCC	4 cut(s)	330, 363, 401, 1339
BsuTUI	ATCGAT	2 cut(s)	318, 408
BtgI	CCRYGG	3 cut(s)	259, 271, 493
BtrI	CACGTC	1 cut(s)	367
BtsCI	GGATG	4 cut(s)	162, 745, 1147, 1198
BtsIMutI	CAGTG	2 cut(s)	946, 1074
Cac8I	GCNNGC	3 cut(s)	143, 256, 355
CaiI	CAGNNNCTG	2 cut(s)	692, 1328
CciI	TCATGA	1 cut(s)	919
CfoI	GCGC	5 cut(s)	19, 66, 129, 340, 858
Cfr13I	GGNCC	4 cut(s)	283, 361, 1263, 1338
Cfr42I	CCGCGG	1 cut(s)	262
ClaI	ATCGAT	2 cut(s)	318, 408
CseI	GACGC	2 cut(s)	265, 289
Csp6I	GTAC	3 cut(s)	333, 645, 1147
CviAII	CATG	3 cut(s)	235, 716, 920
CviQI	GTAC	3 cut(s)	333, 645, 1147
DdeI	CTNAG	5 cut(s)	74, 450, 878, 1104, 1259
DpnI	GATC	4 cut(s)	204, 420, 619, 1109
DpnII	GATC	4 cut(s)	202, 418, 617, 1107
DraI	TTTAAA	1 cut(s)	957
EaeI	YGGCCR	1 cut(s)	328
EagI	CGGCCG	1 cut(s)	328
Eam1104I	CTCTTC	1 cut(s)	1209
EarI	CTCTTC	1 cut(s)	1209
EciI	GGCGGA	1 cut(s)	155
Ecl136II	GAGCTC	1 cut(s)	568
EclXI	CGGCCG	1 cut(s)	328
Eco130I	CCWWGG	2 cut(s)	1064, 1427
Eco24I	GRGCYC	1 cut(s)	570
Eco32I	GATATC	1 cut(s)	36
Eco47I	GGWCC	2 cut(s)	283, 1263
Eco52I	CGGCCG	1 cut(s)	328
Eco53kI	GAGCTC	1 cut(s)	568
Eco57I	CTGAAG	2 cut(s)	44, 115
Eco88I	CYCGRG	3 cut(s)	215, 414, 546
EcoICRI	GAGCTC	1 cut(s)	568
EcoO109I	RGGNCCY	1 cut(s)	283
EcoRII	CCWGG	1 cut(s)	110
EcoRV	GATATC	1 cut(s)	36
EcoT14I	CCWWGG	2 cut(s)	1064, 1427
EcoT38I	GRGCYC	1 cut(s)	570
ErhI	CCWWGG	2 cut(s)	1064, 1427
FaeI	CATG	3 cut(s)	238, 719, 923
FaqI	GGGAC	1 cut(s)	197
FatI	CATG	3 cut(s)	234, 715, 919
FauI	CCCGC	3 cut(s)	21, 98, 401
FblI	GTMKAC	1 cut(s)	303
FokI	GGATG	4 cut(s)	149, 732, 1154, 1185
FriOI	GRGCYC	1 cut(s)	570
FspBI	CTAG	2 cut(s)	378, 1055
GlaI	GCGC	5 cut(s)	18, 65, 128, 339, 857
GsaI	CCCAGC	1 cut(s)	427
GsuI	CTGGAG	1 cut(s)	743
HaeII	RGCGCY	2 cut(s)	341, 859
HaeIII	GGCC	4 cut(s)	330, 363, 401, 1339
HapII	CCGG	1 cut(s)	67
HgaI	GACGC	2 cut(s)	265, 289
HhaI	GCGC	5 cut(s)	19, 66, 129, 340, 858
Hin1I	GRCGYC	4 cut(s)	267, 276, 300, 306
Hin1II	CATG	3 cut(s)	238, 719, 923
Hin6I	GCGC	5 cut(s)	17, 64, 127, 338, 856
HinP1I	GCGC	5 cut(s)	17, 64, 127, 338, 856
HincII	GTYRAC	3 cut(s)	304, 310, 992
HindII	GTYRAC	3 cut(s)	304, 310, 992
HindIII	AAGCTT	1 cut(s)	1421
HinfI	GANTC	4 cut(s)	220, 227, 541, 1318
HpaII	CCGG	1 cut(s)	67
HphI	GGTGA	3 cut(s)	836, 1029, 1120
Hpy166II	GTNNAC	7 cut(s)	271, 304, 310, 475, 929, 992, 1147
Hpy188I	TCNGA	4 cut(s)	486, 510, 808, 1351
Hpy188III	TCNNGA	9 cut(s)	49, 224, 416, 517, 548, 557, 890, 920, 1322
Hpy8I	GTNNAC	7 cut(s)	271, 304, 310, 475, 929, 992, 1147
Hpy99I	CGWCG	9 cut(s)	55, 188, 197, 200, 269, 281, 305, 308, 371
HpyAV	CCTTC	5 cut(s)	186, 643, 730, 1235, 1402
HpyCH4III	ACNGT	3 cut(s)	535, 950, 1365
HpyCH4IV	ACGT	6 cut(s)	198, 267, 306, 366, 588, 1415
HpyCH4V	TGCA	1 cut(s)	899
HpyF10VI	GCNNNNNNNGC	4 cut(s)	25, 429, 446, 1055
HpyF3I	CTNAG	5 cut(s)	74, 450, 878, 1104, 1259
HpySE526I	ACGT	6 cut(s)	198, 267, 306, 366, 588, 1415
Hsp92I	GRCGYC	4 cut(s)	267, 276, 300, 306
Hsp92II	CATG	3 cut(s)	238, 719, 923
HspAI	GCGC	5 cut(s)	17, 64, 127, 338, 856
KspI	CCGCGG	1 cut(s)	262
Kzo9I	GATC	4 cut(s)	202, 418, 617, 1107
LmnI	GCTCC	3 cut(s)	345, 497, 834
Lsp1109I	GCAGC	8 cut(s)	31, 48, 183, 337, 441, 449, 1207, 1267
LweI	GCATC	4 cut(s)	324, 400, 436, 754
MaeI	CTAG	2 cut(s)	378, 1055
MaeII	ACGT	6 cut(s)	198, 267, 306, 366, 588, 1415
MaeIII	GTNAC	2 cut(s)	775, 824
MalI	GATC	4 cut(s)	204, 420, 619, 1109
MboI	GATC	4 cut(s)	202, 418, 617, 1107
MboII	GAAGA	5 cut(s)	532, 833, 908, 1226, 1336
MflI	RGATCY	1 cut(s)	1107
MhlI	GDGCHC	1 cut(s)	570
MluCI	AATT	4 cut(s)	761, 910, 1122, 1384
MlyI	GAGTC	1 cut(s)	221
MmeI	TCCRAC	2 cut(s)	1295, 1347
MseI	TTAA	4 cut(s)	702, 956, 1019, 1419
MslI	CAYNNNNRTG	2 cut(s)	186, 750
MspA1I	CMGCKG	4 cut(s)	22, 261, 440, 1258
MspI	CCGG	1 cut(s)	67
MspR9I	CCNGG	1 cut(s)	112
MvaI	CCWGG	1 cut(s)	112
MvnI	CGCG	2 cut(s)	14, 261
MwoI	GCNNNNNNNGC	4 cut(s)	25, 429, 446, 1055
NdeII	GATC	4 cut(s)	202, 418, 617, 1107
NlaIII	CATG	3 cut(s)	238, 719, 923
NlaIV	GGNNCC	4 cut(s)	71, 499, 772, 1101
NmuCI	GTSAC	1 cut(s)	824
NspV	TTCGAA	3 cut(s)	592, 818, 1382
PaeR7I	CTCGAG	1 cut(s)	546
PagI	TCATGA	1 cut(s)	919
PcsI	WCGNNNNNNNCGW	4 cut(s)	189, 192, 273, 354
PfeI	GAWTC	3 cut(s)	220, 541, 1318
Pfl23II	CGTACG	1 cut(s)	332
PflFI	GACNNNGTC	1 cut(s)	55
PleI	GAGTC	1 cut(s)	221
PpsI	GAGTC	1 cut(s)	221
PpuMI	RGGWCCY	1 cut(s)	283
PshBI	ATTAAT	1 cut(s)	702
Psp124BI	GAGCTC	1 cut(s)	570
Psp5II	RGGWCCY	1 cut(s)	283
Psp6I	CCWGG	1 cut(s)	110
PspFI	CCCAGC	1 cut(s)	423
PspGI	CCWGG	1 cut(s)	110
PspLI	CGTACG	1 cut(s)	332
PspN4I	GGNNCC	4 cut(s)	71, 499, 772, 1101
PspPI	GGNCC	4 cut(s)	283, 361, 1263, 1338
PspPPI	RGGWCCY	1 cut(s)	283
PstNI	CAGNNNCTG	2 cut(s)	692, 1328
PsuI	RGATCY	1 cut(s)	1107
PsyI	GACNNNGTC	1 cut(s)	55
PvuII	CAGCTG	3 cut(s)	22, 440, 1258
RsaI	GTAC	3 cut(s)	334, 646, 1148
RsaNI	GTAC	3 cut(s)	333, 645, 1147
RseI	CAYNNNNRTG	2 cut(s)	186, 750
SacI	GAGCTC	1 cut(s)	570
SacII	CCGCGG	1 cut(s)	262
SalI	GTCGAC	1 cut(s)	302
SaqAI	TTAA	4 cut(s)	702, 956, 1019, 1419
Sau3AI	GATC	4 cut(s)	202, 418, 617, 1107
Sau96I	GGNCC	4 cut(s)	283, 361, 1263, 1338
SchI	GAGTC	1 cut(s)	221
ScrFI	CCNGG	1 cut(s)	112
SduI	GDGCHC	1 cut(s)	570
SfaNI	GCATC	4 cut(s)	324, 400, 436, 754
SfcI	CTRYAG	2 cut(s)	441, 1287
Sfr274I	CTCGAG	1 cut(s)	546
Sfr303I	CCGCGG	1 cut(s)	262
SfuI	TTCGAA	3 cut(s)	592, 818, 1382
SgrBI	CCGCGG	1 cut(s)	262
SgrDI	CGTCGACG	1 cut(s)	302
SinI	GGWCC	2 cut(s)	283, 1263
SlaI	CTCGAG	1 cut(s)	546
SmiMI	CAYNNNNRTG	2 cut(s)	186, 750
SmlI	CTYRAG	1 cut(s)	546
SmoI	CTYRAG	1 cut(s)	546
Sse9I	AATT	4 cut(s)	761, 910, 1122, 1384
SsiI	CCGC	9 cut(s)	14, 28, 91, 166, 169, 259, 261, 394, 1253
SspI	AATATT	2 cut(s)	481, 970
SspMI	CTAG	2 cut(s)	378, 1055
SstI	GAGCTC	1 cut(s)	570
StyD4I	CCNGG	1 cut(s)	110
StyI	CCWWGG	2 cut(s)	1064, 1427
TaaI	ACNGT	3 cut(s)	535, 950, 1365
TaiI	ACGT	6 cut(s)	201, 270, 309, 369, 591, 1418
TaqII	GACCGA	1 cut(s)	1145
TasI	AATT	4 cut(s)	761, 910, 1122, 1384
TatI	WGTACW	1 cut(s)	1146
TauI	GCSGC	4 cut(s)	17, 171, 261, 264
TfiI	GAWTC	3 cut(s)	220, 541, 1318
Tru1I	TTAA	4 cut(s)	702, 956, 1019, 1419
Tru9I	TTAA	4 cut(s)	702, 956, 1019, 1419
TscAI	CASTG	2 cut(s)	953, 1081
TseFI	GTSAC	1 cut(s)	824
TseI	GCWGC	8 cut(s)	19, 61, 171, 350, 429, 437, 1220, 1255
Tsp45I	GTSAC	1 cut(s)	824
TspDTI	ATGAA	4 cut(s)	251, 908, 936, 1134
TspGWI	ACGGA	3 cut(s)	199, 482, 564
TspRI	CASTG	2 cut(s)	953, 1081
Tth111I	GACNNNGTC	1 cut(s)	55
VpaK11BI	GGWCC	2 cut(s)	283, 1263
VspI	ATTAAT	1 cut(s)	702
XapI	RAATTY	1 cut(s)	1122
XhoI	CTCGAG	1 cut(s)	546
XmiI	GTMKAC	1 cut(s)	303
XspI	CTAG	2 cut(s)	378, 1055
ZraI	GACGTC	2 cut(s)	268, 307

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.