Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009864

Physical Location & Seq

Genomic Coordinates Forward (+)

13524048 .. 13528712

Gene Structure 4665 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G31810.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1476 bp

ATGGCGGCCATTTCTGTAAGTTCTTCACCATCTATTCGCTGCTTGAGATCGGCATGTTCCGATTCTTCTCCTGCTCTTGTATCCTCGACGCGTGTATCGTTCCCGGCGAAGATTTCATATCTCTCCGGTATATCTTCGCACCGTGGCGATGAAATGGGTAAGAGAATGGAAGGATTCGTTAGAAGCGTCGATGGGAAGATCTCTGATGCGTCTTTCTCCGAAGCTTCATCTGCGACTCCAAAATCGAAGGTGAGGAAGCACACAATTTCAGTATTTGTTGGAGACGAAAGCGGAATGATTAATAGGATTGCAGGAGTGTTTGCAAGGAGAGGATACAATATTGAGAGTCTTGCTGTTGGTCTGAACAGAGACAAGGCTCTATTCACCATAGTTGTCTGTGGAACTGAAAGGGTACTTCAGCAGGTCATCGAGCAACTCCAGAAGCTCGTTAATGTTCTAAAGGTTGAAGATATCTCAAGTGAGCCGCAAGTGGAGCGTGAGCTGATGCTTGTAAAAGTGAATGCACATCCAGAATCCAGGGCAGAGATCATGTGGCTAGTTGACACATTCAGAGCAAGAGTTGTAGATATAGCGGAACATGCATTGACTATCGAGGTAACTGGAGATCCTGGAAAAATGATTGCTGTAGAAAGAAATTTGAAAAAGTTTCAGATCAGAGAGATTGTAAGGACAGGAAAGATAGCACTGAGAAGGGAAAAGATGGGTGCAACTGCTCCATTTTGGCGATTTTCAGCAGCATCCTATCCAGATCTCAAGGAGCAAGCGCCTGTTAGTGTTCTTCGAAGTAGCAAAAAAGGAGCCATTGTCCCTCAAAAGGAAACATCAGCAGGGGGAGATGTTTATCCCGTTGAGCCATTTTTTGACCCCAAGGTACATCGTATTCTCGACGCTCACTGGGGACTTCTCACTGACGAAGATACGAGTGGACTACGGTCGCATACTCTATCATTGCTTGTAAATGATATTCCAGGAGTTCTTAATATTGTGACTGGTGTTTTCGCTCGAAGGGGATACAATATCCAGAGCTTGGCCGTAGGACATGCTGAAACCAAGGGCATTTCACGCATTACAACAGTTATACCTGCAACAGATGAATCGGTCAGCAAATTGGTGCAGCAACTTTACAAACTCGTAGATGTGCATGAGGTCCATGATCTTACTCATTTGCCATTTTCTGAAAGAGAACTGATGCTGATTAAGATTGCCGTGAACGCTGCTGCTAGAAGAGATGTCCTGGACATTGCTAGTATTTTCAGGGCTAAAGCTGTTGACGTATCTGATCACACAATTACTTTGCAGCTTACTGGGGATCTAGACAAGATGGTTGCACTGCAAAGGTTATTGGAGCCCTATGGTATATGTGAGGTTGCAAGAACCGGTCGTGTGGCATTGGCTCGTGAATCGGGAGTGGACTCCAAGTACCTTCGTGGATACTCCTTTCCTTTAACAGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0005948 GO:0006082 GO:0006520 GO:0006549 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009097 GO:0009099 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0032991 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043621 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0050790 GO:0065007 GO:0065009 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

ACT ACT_5 ALS_ss_C

Protein Analysis

491

Amino Acids

53.87

Weight (kDa)

9.07

Isoelectric Point (pI)

43.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACT	PF01842	87 - 150	1.2e-10	ACT domain
ACT_AHAS_ss	PF22629	89 - 154	6.7e-16	AHAS small subunit-like ACT domain
ACT_5	PF13710	97 - 155	3.1e-08	ACT domain
ALS_ss_C	PF10369	166 - 239	1.9e-27	Small subunit of acetolactate synthase
ACT	PF01842	320 - 384	3e-09	ACT domain
ACT_AHAS_ss	PF22629	322 - 388	2.4e-22	AHAS small subunit-like ACT domain
ACT_5	PF13710	329 - 390	3.1e-09	ACT domain
ALS_ss_C	PF10369	401 - 441	8.9e-11	Small subunit of acetolactate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0009864)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31810 AT2G31810 AT2G31810
fragaria_vesca	FvH4_1g00350 FvH4_1g00350
malus_domestica	MD02G1002700.v1.1 MD15G1145900.v1.1
prunus_persica	Prupe.7G268500_v2.0.a1
pyrus_communis	pycom15g13110
rosa_chinensis	RchiOBHm_Chr2g0084781
rosa_laevigata	RLG00000015638
rosa_multiflora	Rmu_sc0002352.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155820
rosa_rugosa	Rorug01G0456000 Rorug01G0456100 Rorug01G0456200
rosa_samantha	Rh2AG003300 Rh2BG004900 Rh2CG005300 Rh2DG004700
rosa_wichuraiana	Rw2G000380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	412, 1111
AccB7I	CCANNNNNTGG	1 cut(s)	1048
AccII	CGCG	1 cut(s)	91
AciI	CCGC	4 cut(s)	5, 291, 485, 593
AclWI	GGATC	2 cut(s)	620, 1338
AcoI	YGGCCR	2 cut(s)	6, 1050
AcsI	RAATTY	1 cut(s)	655
AcuI	CTGAAG	1 cut(s)	401
AfaI	GTAC	3 cut(s)	414, 894, 1442
AfiI	CCNNNNNNNGG	3 cut(s)	835, 1048, 1469
AflIII	ACRYGT	1 cut(s)	89
AgeI	ACCGGT	1 cut(s)	1397
AgsI	TTSAA	2 cut(s)	467, 661
AjnI	CCWGG	4 cut(s)	536, 628, 988, 1254
AluBI	AGCT	6 cut(s)	224, 445, 502, 1047, 1286, 1321
AluI	AGCT	6 cut(s)	224, 445, 502, 1047, 1286, 1321
Alw26I	GTCTC	2 cut(s)	276, 363
AlwI	GGATC	2 cut(s)	620, 1338
AoxI	GGCC	2 cut(s)	6, 1050
ApeKI	GCWGC	6 cut(s)	39, 755, 1135, 1235, 1238, 1318
ApoI	RAATTY	1 cut(s)	655
AseI	ATTAAT	1 cut(s)	300
AsiGI	ACCGGT	1 cut(s)	1397
AspLEI	GCGC	1 cut(s)	787
AspS9I	GGNCC	1 cut(s)	1168
AsuC2I	CCSGG	1 cut(s)	104
AsuHPI	GGTGA	3 cut(s)	18, 262, 376
AsuII	TTCGAA	1 cut(s)	802
AvaII	GGWCC	1 cut(s)	1168
BaeI	ACNNNNGTAYC	2 cut(s)	78, 111
BanII	GRGCYC	1 cut(s)	1371
BauI	CACGAG	1 cut(s)	1416
BbvI	GCAGC	6 cut(s)	26, 767, 1147, 1222, 1225, 1330
BccI	CCATC	4 cut(s)	37, 185, 715, 1336
BceAI	ACGGC	2 cut(s)	1037, 1211
BciT130I	CCWGG	4 cut(s)	538, 630, 990, 1256
BciVI	GTATCC	4 cut(s)	91, 326, 1025, 1445
BclI	TGATCA	1 cut(s)	1300
BcnI	CCSGG	1 cut(s)	104
BcoDI	GTCTC	2 cut(s)	276, 363
BfaI	CTAG	4 cut(s)	557, 1242, 1266, 1334
BfmI	CTRYAG	1 cut(s)	645
BfoI	RGCGCY	1 cut(s)	788
BfuAI	ACCTGC	2 cut(s)	412, 1111
BfuI	GTATCC	4 cut(s)	91, 326, 1025, 1445
BglII	AGATCT	2 cut(s)	198, 769
BisI	GCNGC	8 cut(s)	6, 40, 485, 756, 1136, 1236, 1239, 1319
BlsI	GCNGC	8 cut(s)	7, 41, 486, 757, 1137, 1237, 1240, 1320
Bme1390I	CCNGG	5 cut(s)	104, 538, 630, 990, 1256
Bme18I	GGWCC	1 cut(s)	1168
BmgT120I	GGNCC	1 cut(s)	1168
BmiI	GGNNCC	2 cut(s)	820, 1368
BmrFI	CCNGG	5 cut(s)	104, 538, 630, 990, 1256
BmrI	ACTGGG	2 cut(s)	925, 1335
BmsI	GCATC	4 cut(s)	196, 495, 767, 1200
BmuI	ACTGGG	2 cut(s)	925, 1335
BoxI	GACNNNNGTC	1 cut(s)	952
BpmI	CTGGAG	2 cut(s)	422, 642
Bpu14I	TTCGAA	1 cut(s)	802
BpuEI	CTTGAG	3 cut(s)	64, 460, 758
BpuMI	CCSGG	1 cut(s)	104
BsaBI	GATNNNNATC	1 cut(s)	861
BsaJI	CCNNGG	4 cut(s)	142, 537, 888, 1071
BsaWI	WCCGGW	2 cut(s)	125, 1397
BsaXI	ACNNNNNCTCC	2 cut(s)	810, 840
Bsc4I	CCNNNNNNNGG	3 cut(s)	835, 1048, 1469
Bse118I	RCCGGY	1 cut(s)	1397
Bse1I	ACTGG	4 cut(s)	625, 920, 1015, 1330
Bse3DI	GCAATG	2 cut(s)	968, 1260
Bse8I	GATNNNNATC	1 cut(s)	861
BseBI	CCWGG	4 cut(s)	538, 630, 990, 1256
BseDI	CCNNGG	4 cut(s)	142, 537, 888, 1071
BseGI	GGATG	2 cut(s)	526, 758
BseJI	GATNNNNATC	1 cut(s)	861
BseLI	CCNNNNNNNGG	3 cut(s)	835, 1048, 1469
BseMI	GCAATG	2 cut(s)	968, 1260
BseMII	CTCAG	1 cut(s)	698
BseNI	ACTGG	4 cut(s)	625, 920, 1015, 1330
BseXI	GCAGC	6 cut(s)	26, 767, 1147, 1222, 1225, 1330
BsgI	GTGCAG	1 cut(s)	1154
Bsh1236I	CGCG	1 cut(s)	91
Bsh1285I	CGRYCG	2 cut(s)	956, 1402
BshFI	GGCC	2 cut(s)	8, 1052
BshTI	ACCGGT	1 cut(s)	1397
BsiEI	CGRYCG	2 cut(s)	956, 1402
BsiSI	CCGG	3 cut(s)	104, 126, 1398
BslFI	GGGAC	2 cut(s)	812, 933
BslI	CCNNNNNNNGG	3 cut(s)	835, 1048, 1469
BsmAI	GTCTC	2 cut(s)	276, 363
BsmBI	CGTCTC	1 cut(s)	276
BsmFI	GGGAC	2 cut(s)	812, 933
BsmI	GAATGC	1 cut(s)	526
BsnI	GGCC	2 cut(s)	8, 1052
Bsp119I	TTCGAA	1 cut(s)	802
Bsp1286I	GDGCHC	1 cut(s)	1371
Bsp143I	GATC	9 cut(s)	47, 198, 546, 625, 672, 769, 1174, 1300, 1330
BspACI	CCGC	4 cut(s)	5, 291, 485, 593
BspANI	GGCC	2 cut(s)	8, 1052
BspCNI	CTCAG	1 cut(s)	699
BspFNI	CGCG	1 cut(s)	91
BspLI	GGNNCC	2 cut(s)	820, 1368
BspMI	ACCTGC	2 cut(s)	412, 1111
BspPI	GGATC	2 cut(s)	620, 1338
BspT104I	TTCGAA	1 cut(s)	802
BsrDI	GCAATG	2 cut(s)	968, 1260
BsrFI	RCCGGY	1 cut(s)	1397
BsrI	ACTGG	4 cut(s)	625, 920, 1015, 1330
BssAI	RCCGGY	1 cut(s)	1397
BssECI	CCNNGG	4 cut(s)	142, 537, 888, 1071
BssMI	GATC	9 cut(s)	47, 198, 546, 625, 672, 769, 1174, 1300, 1330
BssSI	CACGAG	1 cut(s)	1416
BssT1I	CCWWGG	2 cut(s)	888, 1071
Bst2BI	CACGAG	1 cut(s)	1416
Bst2UI	CCWGG	4 cut(s)	538, 630, 990, 1256
Bst4CI	ACNGT	3 cut(s)	143, 954, 1096
Bst6I	CTCTTC	1 cut(s)	1240
BstBI	TTCGAA	1 cut(s)	802
BstC8I	GCNNGC	1 cut(s)	783
BstDEI	CTNAG	1 cut(s)	707
BstDSI	CCRYGG	1 cut(s)	142
BstENI	CCTNNNNNAGG	1 cut(s)	1467
BstF5I	GGATG	2 cut(s)	526, 758
BstFNI	CGCG	1 cut(s)	91
BstH2I	RGCGCY	1 cut(s)	788
BstHHI	GCGC	1 cut(s)	787
BstKTI	GATC	9 cut(s)	50, 201, 549, 628, 675, 772, 1177, 1303, 1333
BstMAI	GTCTC	2 cut(s)	276, 363
BstMBI	GATC	9 cut(s)	47, 198, 546, 625, 672, 769, 1174, 1300, 1330
BstMCI	CGRYCG	2 cut(s)	956, 1402
BstMWI	GCNNNNNNNGC	5 cut(s)	230, 493, 599, 1083, 1232
BstNI	CCWGG	4 cut(s)	538, 630, 990, 1256
BstNSI	RCATGY	3 cut(s)	57, 602, 1064
BstPAI	GACNNNNGTC	1 cut(s)	952
BstSCI	CCNGG	5 cut(s)	102, 536, 628, 988, 1254
BstSFI	CTRYAG	1 cut(s)	645
BstUI	CGCG	1 cut(s)	91
BstV1I	GCAGC	6 cut(s)	26, 767, 1147, 1222, 1225, 1330
BstX2I	RGATCY	4 cut(s)	198, 625, 769, 1330
BstYI	RGATCY	4 cut(s)	198, 625, 769, 1330
BsuI	GTATCC	4 cut(s)	91, 326, 1025, 1445
BsuRI	GGCC	2 cut(s)	8, 1052
BtgI	CCRYGG	1 cut(s)	142
BtgZI	GCGATG	1 cut(s)	162
BtsCI	GGATG	2 cut(s)	526, 758
BtsI	GCAGTG	1 cut(s)	1349
BtsIMutI	CAGTG	4 cut(s)	704, 913, 927, 1349
BveI	ACCTGC	2 cut(s)	412, 1111
Cac8I	GCNNGC	1 cut(s)	783
CfoI	GCGC	1 cut(s)	787
Cfr10I	RCCGGY	1 cut(s)	1397
Cfr13I	GGNCC	1 cut(s)	1168
CseI	GACGC	4 cut(s)	97, 175, 198, 917
Csp6I	GTAC	3 cut(s)	413, 893, 1441
CspAI	ACCGGT	1 cut(s)	1397
CviAII	CATG	6 cut(s)	54, 550, 599, 1061, 1163, 1172
CviQI	GTAC	3 cut(s)	413, 893, 1441
DdeI	CTNAG	1 cut(s)	707
DpnI	GATC	9 cut(s)	49, 200, 548, 627, 674, 771, 1176, 1302, 1332
DpnII	GATC	9 cut(s)	47, 198, 546, 625, 672, 769, 1174, 1300, 1330
EaeI	YGGCCR	2 cut(s)	6, 1050
Eam1104I	CTCTTC	1 cut(s)	1240
EarI	CTCTTC	1 cut(s)	1240
Eco130I	CCWWGG	2 cut(s)	888, 1071
Eco24I	GRGCYC	1 cut(s)	1371
Eco32I	GATATC	1 cut(s)	472
Eco47I	GGWCC	1 cut(s)	1168
Eco57I	CTGAAG	1 cut(s)	401
EcoNI	CCTNNNNNAGG	1 cut(s)	1467
EcoRII	CCWGG	4 cut(s)	536, 628, 988, 1254
EcoRV	GATATC	1 cut(s)	472
EcoT14I	CCWWGG	2 cut(s)	888, 1071
EcoT22I	ATGCAT	1 cut(s)	604
EcoT38I	GRGCYC	1 cut(s)	1371
ErhI	CCWWGG	2 cut(s)	888, 1071
Esp3I	CGTCTC	1 cut(s)	276
FaeI	CATG	6 cut(s)	57, 553, 602, 1064, 1166, 1175
FaqI	GGGAC	2 cut(s)	812, 933
FatI	CATG	6 cut(s)	53, 549, 598, 1060, 1162, 1171
FbaI	TGATCA	1 cut(s)	1300
Fnu4HI	GCNGC	8 cut(s)	6, 40, 485, 756, 1136, 1236, 1239, 1319
FokI	GGATG	2 cut(s)	513, 745
FriOI	GRGCYC	1 cut(s)	1371
Fsp4HI	GCNGC	8 cut(s)	6, 40, 485, 756, 1136, 1236, 1239, 1319
FspBI	CTAG	4 cut(s)	557, 1242, 1266, 1334
GlaI	GCGC	1 cut(s)	786
GluI	GCNGC	8 cut(s)	6, 40, 485, 756, 1136, 1236, 1239, 1319
GsuI	CTGGAG	2 cut(s)	422, 642
HaeII	RGCGCY	1 cut(s)	788
HaeIII	GGCC	2 cut(s)	8, 1052
HapII	CCGG	3 cut(s)	104, 126, 1398
HgaI	GACGC	4 cut(s)	97, 175, 198, 917
HhaI	GCGC	1 cut(s)	787
Hin1II	CATG	6 cut(s)	57, 553, 602, 1064, 1166, 1175
Hin6I	GCGC	1 cut(s)	785
HinP1I	GCGC	1 cut(s)	785
HincII	GTYRAC	2 cut(s)	562, 1291
HindII	GTYRAC	2 cut(s)	562, 1291
HindIII	AAGCTT	1 cut(s)	222
HinfI	GANTC	8 cut(s)	62, 174, 235, 346, 533, 1115, 1421, 1433
HpaII	CCGG	3 cut(s)	104, 126, 1398
HphI	GGTGA	3 cut(s)	18, 262, 376
Hpy166II	GTNNAC	5 cut(s)	562, 947, 1231, 1291, 1432
Hpy188I	TCNGA	9 cut(s)	61, 205, 220, 363, 572, 672, 677, 1198, 1300
Hpy188III	TCNNGA	8 cut(s)	439, 530, 767, 905, 1042, 1334, 1418, 1425
Hpy8I	GTNNAC	5 cut(s)	562, 947, 1231, 1291, 1432
Hpy99I	CGWCG	3 cut(s)	91, 191, 911
HpyAV	CCTTC	5 cut(s)	164, 241, 705, 1020, 1454
HpyCH4III	ACNGT	3 cut(s)	143, 954, 1096
HpyCH4IV	ACGT	1 cut(s)	1293
HpyF10VI	GCNNNNNNNGC	5 cut(s)	230, 493, 599, 1083, 1232
HpyF3I	CTNAG	1 cut(s)	707
HpySE526I	ACGT	1 cut(s)	1293
Hsp92II	CATG	6 cut(s)	57, 553, 602, 1064, 1166, 1175
HspAI	GCGC	1 cut(s)	785
Ksp22I	TGATCA	1 cut(s)	1300
Kzo9I	GATC	9 cut(s)	47, 198, 546, 625, 672, 769, 1174, 1300, 1330
LmnI	GCTCC	5 cut(s)	493, 739, 778, 818, 1366
Lsp1109I	GCAGC	6 cut(s)	26, 767, 1147, 1222, 1225, 1330
LweI	GCATC	4 cut(s)	196, 495, 767, 1200
MaeI	CTAG	4 cut(s)	557, 1242, 1266, 1334
MaeII	ACGT	1 cut(s)	1293
MaeIII	GTNAC	2 cut(s)	616, 1006
MalI	GATC	9 cut(s)	49, 200, 548, 627, 674, 771, 1176, 1302, 1332
MboI	GATC	9 cut(s)	47, 198, 546, 625, 672, 769, 1174, 1300, 1330
MboII	GAAGA	9 cut(s)	15, 57, 121, 126, 208, 479, 791, 947, 1257
MflI	RGATCY	4 cut(s)	198, 625, 769, 1330
MhlI	GDGCHC	1 cut(s)	1371
MluCI	AATT	4 cut(s)	264, 655, 1127, 1308
MluI	ACGCGT	1 cut(s)	89
MlyI	GAGTC	3 cut(s)	229, 355, 1427
MmeI	TCCRAC	1 cut(s)	259
MnlI	CCTC	7 cut(s)	94, 246, 323, 607, 840, 1159, 1378
Mph1103I	ATGCAT	1 cut(s)	604
MseI	TTAA	5 cut(s)	300, 450, 999, 1218, 1466
MspI	CCGG	3 cut(s)	104, 126, 1398
MspR9I	CCNGG	5 cut(s)	104, 538, 630, 990, 1256
Mva1269I	GAATGC	1 cut(s)	526
MvaI	CCWGG	4 cut(s)	538, 630, 990, 1256
MvnI	CGCG	1 cut(s)	91
MwoI	GCNNNNNNNGC	5 cut(s)	230, 493, 599, 1083, 1232
NciI	CCSGG	1 cut(s)	104
NdeII	GATC	9 cut(s)	47, 198, 546, 625, 672, 769, 1174, 1300, 1330
NlaIII	CATG	6 cut(s)	57, 553, 602, 1064, 1166, 1175
NlaIV	GGNNCC	2 cut(s)	820, 1368
NmuCI	GTSAC	1 cut(s)	1006
NsiI	ATGCAT	1 cut(s)	604
NspI	RCATGY	3 cut(s)	57, 602, 1064
NspV	TTCGAA	1 cut(s)	802
PcsI	WCGNNNNNNNCGW	3 cut(s)	95, 104, 183
PctI	GAATGC	1 cut(s)	526
PfeI	GAWTC	5 cut(s)	62, 174, 533, 1115, 1421
PflMI	CCANNNNNTGG	1 cut(s)	1048
PfoI	TCCNGGA	3 cut(s)	628, 988, 1254
PinAI	ACCGGT	1 cut(s)	1397
PkrI	GCNGC	8 cut(s)	7, 41, 486, 757, 1137, 1237, 1240, 1320
PleI	GAGTC	3 cut(s)	229, 354, 1427
PpsI	GAGTC	3 cut(s)	229, 354, 1427
PshAI	GACNNNNGTC	1 cut(s)	952
PshBI	ATTAAT	1 cut(s)	300
Psp6I	CCWGG	4 cut(s)	536, 628, 988, 1254
PspGI	CCWGG	4 cut(s)	536, 628, 988, 1254
PspN4I	GGNNCC	2 cut(s)	820, 1368
PspPI	GGNCC	1 cut(s)	1168
PsuI	RGATCY	4 cut(s)	198, 625, 769, 1330
RsaI	GTAC	3 cut(s)	414, 894, 1442
RsaNI	GTAC	3 cut(s)	413, 893, 1441
SaqAI	TTAA	5 cut(s)	300, 450, 999, 1218, 1466
SatI	GCNGC	8 cut(s)	6, 40, 485, 756, 1136, 1236, 1239, 1319
Sau3AI	GATC	9 cut(s)	47, 198, 546, 625, 672, 769, 1174, 1300, 1330
Sau96I	GGNCC	1 cut(s)	1168
SchI	GAGTC	3 cut(s)	229, 355, 1427
ScrFI	CCNGG	5 cut(s)	104, 538, 630, 990, 1256
SduI	GDGCHC	1 cut(s)	1371
SfaNI	GCATC	4 cut(s)	196, 495, 767, 1200
SfcI	CTRYAG	1 cut(s)	645
SfuI	TTCGAA	1 cut(s)	802
SinI	GGWCC	1 cut(s)	1168
SmlI	CTYRAG	3 cut(s)	43, 475, 773
SmoI	CTYRAG	3 cut(s)	43, 475, 773
Sse9I	AATT	4 cut(s)	264, 655, 1127, 1308
SsiI	CCGC	4 cut(s)	5, 291, 485, 593
SspI	AATATT	2 cut(s)	340, 1003
SspMI	CTAG	4 cut(s)	557, 1242, 1266, 1334
StyD4I	CCNGG	5 cut(s)	102, 536, 628, 988, 1254
StyI	CCWWGG	2 cut(s)	888, 1071
TaaI	ACNGT	3 cut(s)	143, 954, 1096
TaiI	ACGT	1 cut(s)	1296
TaqI	TCGA	8 cut(s)	86, 189, 245, 429, 612, 802, 906, 1024
TaqII	GACCGA	1 cut(s)	1108
TasI	AATT	4 cut(s)	264, 655, 1127, 1308
TauI	GCSGC	2 cut(s)	8, 487
TfiI	GAWTC	5 cut(s)	62, 174, 533, 1115, 1421
Tru1I	TTAA	5 cut(s)	300, 450, 999, 1218, 1466
Tru9I	TTAA	5 cut(s)	300, 450, 999, 1218, 1466
TscAI	CASTG	4 cut(s)	711, 920, 934, 1356
TseFI	GTSAC	1 cut(s)	1006
TseI	GCWGC	6 cut(s)	39, 755, 1135, 1235, 1238, 1318
Tsp45I	GTSAC	1 cut(s)	1006
TspDTI	ATGAA	4 cut(s)	105, 165, 216, 1128
TspRI	CASTG	4 cut(s)	711, 920, 934, 1356
Van91I	CCANNNNNTGG	1 cut(s)	1048
VpaK11BI	GGWCC	1 cut(s)	1168
VspI	ATTAAT	1 cut(s)	300
XagI	CCTNNNNNAGG	1 cut(s)	1467
XapI	RAATTY	1 cut(s)	655
XbaI	TCTAGA	1 cut(s)	1333
XceI	RCATGY	3 cut(s)	57, 602, 1064
XspI	CTAG	4 cut(s)	557, 1242, 1266, 1334
Zsp2I	ATGCAT	1 cut(s)	604

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.