Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009864

Physical Location & Seq

Genomic Coordinates Reverse (-)

10858593 .. 10862933

Gene Structure 4341 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1145900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1479 bp

ATGGCGACTATTTCTTCTGCTCCGATTCGCACTCCGAACTTCTCTTCGTCCTCCTCCTCTTCCTCCTCGGAGCACCTCCAATTCCTCAGCTTCTCTGAAACGCTATCGTTTCCTCTGACGAGTTCAGCTTCGAGATCAATAAGCCACAGCCATGGCCGCCCCAAGCAACTCGCCGTCTCCGCTAGAAGCGTCGACGAGAATATCGCCGATAACGCCTTCTCTTCCAACGGCTCTCCTCCTTCCACCACCCGCTCCAAGGTGAGAAGGCACACGATTTCGGTGTTCGTTGGGGATGAAAGTGGAATGATAAATCGGATTGCGGGAGTGTTCGCTCGGAGGGGATACAACATCGAGTCCCTGGCTGTTGGTCTGAACAGGGACAAAGCTCTCTTCACTATCGTCGTCTCCGGGACCGAAAGGGTCTTACGCCAAGTAATCGAGCAGCTTAACAAGCTCGTCAATGTTATGAAGGTTGAAGATATCTCAAATGAGCCGCAGGTAGAGCGCGAACTAGTGCTTATAAAAGTAAATTCCGATCCAAGATATCATGCCGAGATCAAGTGGTTAGTGAGTATCTTTAGAGCGAAGATTGTGGATATCTCGGAACACTCAGTAACCATTGAGGTGACAGGAGATCCTGGGAAGATGGTTGCTCTGCAAGGAAATTTAAGCAAGTTTGGGATCAGAGAAGTTGCCAGAACCGGAAAGATTGCCTTGAGGAGGGAAAAGTTGGGAGCATCTGCTCCATTTTGGCGATATTCAGCAGCTTCATATCCTGATCTTGAGAGAACAGTTCCTGTTGATGCTCTTGTAGGGGTTGAAAACGGATTAGTTAATGCTGAATCTGATGTGTCTTCTGAGGGTGATGTTTATCCTGTGGAGCCATCTGATACCTTTACTGTCAAGCAAGTTCTTGATGCTCACTGGGGTGTTCTCAATGATGAAGATACTAATGGCCTTCGATCGCACACTTTATCCATGCTTGTAAATGATGTTCCTGGGGTTCTCTACATTCTTACTGGCGTTTTTGCTCGAAGGGGTTATAACATTCAGAGTTTAGCTGTTGGCCATGCGGAAGTTGAGGGGCAATCTCGCATTACTACTGTTGTTCCTGGTACAGATGAATCAATTAGCAAGTTGGTGCAGCAGCTTTATAAGCTTGTCGATATTCATAATGTTCAAGATCTTACCAATTTGCCATTTGCTGAGCGGGAACTGATGTTGATTAAAATTGCTGTGGATGCTGTCGCTCGAAGAGATGTACTTGATATTGGCAAGATTTTTAGGGCCAAGGCTGTTGACGTTTCTGACCACACAATAACCCTTGAGCTTACGGGAGACTTAAACAAGATGGTTGCATTGCAGAGATTGTTAGAGCCTTATGGAATTTGTGAGGTGGCGCGTACTGGACGAATTGCACTGGTGCGTGAGTCGGGTGTGGATTCGAAACACCTCCGTGGATATTCTTTGCCTCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0005948 GO:0006082 GO:0006520 GO:0006549 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009097 GO:0009099 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0032991 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043621 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0050790 GO:0065007 GO:0065009 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

ACT ACT_5 ALS_ss_C

Protein Analysis

493

Amino Acids

53.9

Weight (kDa)

6.48

Isoelectric Point (pI)

42.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACT	PF01842	90 - 153	1.2e-10	ACT domain
ACT_AHAS_ss	PF22629	92 - 157	3.1e-16	AHAS small subunit-like ACT domain
ACT_5	PF13710	100 - 158	1.9e-08	ACT domain
ALS_ss_C	PF10369	169 - 242	3.1e-26	Small subunit of acetolactate synthase
ACT	PF01842	323 - 387	5.2e-08	ACT domain
ACT_AHAS_ss	PF22629	325 - 391	1.8e-20	AHAS small subunit-like ACT domain
ACT_5	PF13710	332 - 393	7.2e-10	ACT domain
ALS_ss_C	PF10369	404 - 476	9.2e-26	Small subunit of acetolactate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009864)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31810 AT2G31810 AT2G31810
fragaria_vesca	FvH4_1g00350 FvH4_1g00350
malus_domestica	MD02G1002700.v1.1 MD15G1145900.v1.1
prunus_persica	Prupe.7G268500_v2.0.a1
pyrus_communis	pycom15g13110
rosa_chinensis	RchiOBHm_Chr2g0084781
rosa_laevigata	RLG00000015638
rosa_multiflora	Rmu_sc0002352.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155820
rosa_rugosa	Rorug01G0456000 Rorug01G0456100 Rorug01G0456200
rosa_samantha	Rh2AG003300 Rh2BG004900 Rh2CG005300 Rh2DG004700
rosa_wichuraiana	Rw2G000380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	521, 1044, 1155
Acc36I	ACCTGC	1 cut(s)	487
AccBSI	CCGCTC	2 cut(s)	252, 1210
AccI	GTMKAC	1 cut(s)	192
AccII	CGCG	2 cut(s)	507, 1402
AciI	CCGC	7 cut(s)	157, 180, 250, 320, 494, 1073, 1210
AclWI	GGATC	3 cut(s)	530, 629, 689
AcoI	YGGCCR	2 cut(s)	154, 1066
AcsI	RAATTY	3 cut(s)	529, 664, 1386
AfaI	GTAC	3 cut(s)	1117, 1263, 1405
AfiI	CCNNNNNNNGG	2 cut(s)	256, 720
AgsI	TTSAA	3 cut(s)	476, 821, 1181
AhlI	ACTAGT	1 cut(s)	511
AjnI	CCWGG	4 cut(s)	357, 637, 997, 1111
AleI	CACNNNNGTG	2 cut(s)	927, 1455
Alw21I	GWGCWC	1 cut(s)	75
Alw26I	GTCTC	3 cut(s)	181, 409, 1332
AlwI	GGATC	3 cut(s)	530, 629, 689
AlwNI	CAGNNNCTG	1 cut(s)	797
AoxI	GGCC	4 cut(s)	154, 955, 1066, 1287
ApeKI	GCWGC	4 cut(s)	442, 764, 1144, 1147
ApoI	RAATTY	3 cut(s)	529, 664, 1386
AspLEI	GCGC	2 cut(s)	507, 1402
AspS9I	GGNCC	2 cut(s)	411, 1287
AsuC2I	CCSGG	1 cut(s)	409
AsuHPI	GGTGA	3 cut(s)	271, 637, 875
AsuII	TTCGAA	1 cut(s)	1445
AvaII	GGWCC	1 cut(s)	411
BalI	TGGCCA	1 cut(s)	1068
BbsI	GAAGAC	1 cut(s)	846
Bbv12I	GWGCWC	1 cut(s)	75
BbvCI	CCTCAGC	1 cut(s)	86
BbvI	GCAGC	4 cut(s)	454, 776, 1156, 1159
BccI	CCATC	3 cut(s)	640, 892, 1345
BceAI	ACGGC	2 cut(s)	158, 244
BciT130I	CCWGG	4 cut(s)	359, 639, 999, 1113
BciVI	GTATCC	1 cut(s)	335
BcnI	CCSGG	1 cut(s)	409
BcoDI	GTCTC	3 cut(s)	181, 409, 1332
BcuI	ACTAGT	1 cut(s)	511
BfaI	CTAG	2 cut(s)	183, 512
BfmI	CTRYAG	1 cut(s)	1475
BfuAI	ACCTGC	1 cut(s)	487
BfuI	GTATCC	1 cut(s)	335
BglII	AGATCT	1 cut(s)	1183
BisI	GCNGC	6 cut(s)	157, 443, 494, 765, 1145, 1148
BlpI	GCTNAGC	1 cut(s)	1206
BlsI	GCNGC	6 cut(s)	158, 444, 495, 766, 1146, 1149
Bme1390I	CCNGG	5 cut(s)	359, 409, 639, 999, 1113
Bme18I	GGWCC	1 cut(s)	411
BmgT120I	GGNCC	2 cut(s)	411, 1287
BmiI	GGNNCC	2 cut(s)	412, 882
BmrFI	CCNGG	5 cut(s)	359, 409, 639, 999, 1113
BmrI	ACTGGG	1 cut(s)	934
BmsI	GCATC	4 cut(s)	746, 793, 907, 1231
BmuI	ACTGGG	1 cut(s)	934
BpiI	GAAGAC	1 cut(s)	846
Bpu10I	CCTNAGC	1 cut(s)	86
Bpu1102I	GCTNAGC	1 cut(s)	1206
Bpu14I	TTCGAA	1 cut(s)	1445
BpuEI	CTTGAG	3 cut(s)	736, 803, 1346
BpuMI	CCSGG	1 cut(s)	409
BsaBI	GATNNNNATC	1 cut(s)	870
BsaJI	CCNNGG	8 cut(s)	66, 151, 255, 357, 638, 998, 1290, 1456
BsaWI	WCCGGW	1 cut(s)	701
Bsc4I	CCNNNNNNNGG	2 cut(s)	256, 720
Bse1I	ACTGG	4 cut(s)	929, 1024, 1411, 1425
Bse3DI	GCAATG	1 cut(s)	1358
Bse8I	GATNNNNATC	1 cut(s)	870
BseBI	CCWGG	4 cut(s)	359, 639, 999, 1113
BseDI	CCNNGG	8 cut(s)	66, 151, 255, 357, 638, 998, 1290, 1456
BseGI	GGATG	2 cut(s)	298, 1246
BseJI	GATNNNNATC	1 cut(s)	870
BseLI	CCNNNNNNNGG	2 cut(s)	256, 720
BseMI	GCAATG	1 cut(s)	1358
BseMII	CTCAG	4 cut(s)	100, 624, 849, 1197
BseNI	ACTGG	4 cut(s)	929, 1024, 1411, 1425
BseRI	GAGGAG	5 cut(s)	43, 46, 55, 225, 733
BseXI	GCAGC	4 cut(s)	454, 776, 1156, 1159
BsgI	GTGCAG	1 cut(s)	1163
Bsh1236I	CGCG	2 cut(s)	507, 1402
Bsh1285I	CGRYCG	1 cut(s)	965
BshFI	GGCC	4 cut(s)	156, 957, 1068, 1289
BsiEI	CGRYCG	1 cut(s)	965
BsiHKAI	GWGCWC	1 cut(s)	75
BsiSI	CCGG	2 cut(s)	408, 702
BslFI	GGGAC	3 cut(s)	340, 392, 424
BslI	CCNNNNNNNGG	2 cut(s)	256, 720
BsmAI	GTCTC	3 cut(s)	181, 409, 1332
BsmBI	CGTCTC	2 cut(s)	181, 409
BsmFI	GGGAC	3 cut(s)	340, 392, 424
BsnI	GGCC	4 cut(s)	156, 957, 1068, 1289
Bsp119I	TTCGAA	1 cut(s)	1445
Bsp1286I	GDGCHC	1 cut(s)	75
Bsp143I	GATC	8 cut(s)	134, 535, 555, 634, 681, 778, 962, 1183
Bsp1720I	GCTNAGC	1 cut(s)	1206
Bsp19I	CCATGG	1 cut(s)	151
BspACI	CCGC	7 cut(s)	157, 180, 250, 320, 494, 1073, 1210
BspANI	GGCC	4 cut(s)	156, 957, 1068, 1289
BspCNI	CTCAG	4 cut(s)	99, 623, 850, 1198
BspFNI	CGCG	2 cut(s)	507, 1402
BspLI	GGNNCC	2 cut(s)	412, 882
BspMI	ACCTGC	1 cut(s)	487
BspPI	GGATC	3 cut(s)	530, 629, 689
BspT104I	TTCGAA	1 cut(s)	1445
BsrBI	CCGCTC	2 cut(s)	252, 1210
BsrDI	GCAATG	1 cut(s)	1358
BsrI	ACTGG	4 cut(s)	929, 1024, 1411, 1425
BssECI	CCNNGG	8 cut(s)	66, 151, 255, 357, 638, 998, 1290, 1456
BssMI	GATC	8 cut(s)	134, 535, 555, 634, 681, 778, 962, 1183
BssT1I	CCWWGG	3 cut(s)	151, 255, 1290
Bst2UI	CCWGG	4 cut(s)	359, 639, 999, 1113
Bst4CI	ACNGT	3 cut(s)	793, 901, 1105
Bst6I	CTCTTC	5 cut(s)	49, 64, 226, 395, 1249
BstBI	TTCGAA	1 cut(s)	1445
BstDEI	CTNAG	4 cut(s)	86, 610, 858, 1206
BstDSI	CCRYGG	2 cut(s)	151, 1456
BstENI	CCTNNNNNAGG	1 cut(s)	718
BstF5I	GGATG	2 cut(s)	298, 1246
BstFNI	CGCG	2 cut(s)	507, 1402
BstHHI	GCGC	2 cut(s)	507, 1402
BstKTI	GATC	8 cut(s)	137, 538, 558, 637, 684, 781, 965, 1186
BstMAI	GTCTC	3 cut(s)	181, 409, 1332
BstMBI	GATC	8 cut(s)	134, 535, 555, 634, 681, 778, 962, 1183
BstMCI	CGRYCG	1 cut(s)	965
BstMWI	GCNNNNNNNGC	7 cut(s)	156, 179, 212, 451, 502, 1156, 1241
BstNI	CCWGG	4 cut(s)	359, 639, 999, 1113
BstSCI	CCNGG	5 cut(s)	357, 407, 637, 997, 1111
BstSFI	CTRYAG	1 cut(s)	1475
BstUI	CGCG	2 cut(s)	507, 1402
BstV1I	GCAGC	4 cut(s)	454, 776, 1156, 1159
BstV2I	GAAGAC	1 cut(s)	846
BstX2I	RGATCY	2 cut(s)	634, 1183
BstXI	CCANNNNNNTGG	1 cut(s)	152
BstYI	RGATCY	2 cut(s)	634, 1183
BsuI	GTATCC	1 cut(s)	335
BsuRI	GGCC	4 cut(s)	156, 957, 1068, 1289
BtgI	CCRYGG	2 cut(s)	151, 1456
BtsCI	GGATG	2 cut(s)	298, 1246
BtsIMutI	CAGTG	2 cut(s)	922, 1418
BveI	ACCTGC	1 cut(s)	487
CaiI	CAGNNNCTG	1 cut(s)	797
CfoI	GCGC	2 cut(s)	507, 1402
Cfr13I	GGNCC	2 cut(s)	411, 1287
CseI	GACGC	1 cut(s)	178
Csp6I	GTAC	3 cut(s)	1116, 1262, 1404
CviAII	CATG	4 cut(s)	152, 548, 979, 1070
CviQI	GTAC	3 cut(s)	1116, 1262, 1404
DdeI	CTNAG	4 cut(s)	86, 610, 858, 1206
DpnI	GATC	8 cut(s)	136, 537, 557, 636, 683, 780, 964, 1185
DpnII	GATC	8 cut(s)	134, 535, 555, 634, 681, 778, 962, 1183
EaeI	YGGCCR	2 cut(s)	154, 1066
Eam1104I	CTCTTC	5 cut(s)	49, 64, 226, 395, 1249
EarI	CTCTTC	5 cut(s)	49, 64, 226, 395, 1249
Eco130I	CCWWGG	3 cut(s)	151, 255, 1290
Eco32I	GATATC	3 cut(s)	481, 545, 598
Eco47I	GGWCC	1 cut(s)	411
EcoNI	CCTNNNNNAGG	1 cut(s)	718
EcoRII	CCWGG	4 cut(s)	357, 637, 997, 1111
EcoRV	GATATC	3 cut(s)	481, 545, 598
EcoT14I	CCWWGG	3 cut(s)	151, 255, 1290
ErhI	CCWWGG	3 cut(s)	151, 255, 1290
Esp3I	CGTCTC	2 cut(s)	181, 409
FaeI	CATG	4 cut(s)	155, 551, 982, 1073
FalI	AAGNNNNNCTT	2 cut(s)	698, 730
FaqI	GGGAC	3 cut(s)	340, 392, 424
FatI	CATG	4 cut(s)	151, 547, 978, 1069
FauI	CCCGC	3 cut(s)	257, 313, 1203
FblI	GTMKAC	1 cut(s)	192
Fnu4HI	GCNGC	6 cut(s)	157, 443, 494, 765, 1145, 1148
FokI	GGATG	2 cut(s)	305, 1253
Fsp4HI	GCNGC	6 cut(s)	157, 443, 494, 765, 1145, 1148
FspBI	CTAG	2 cut(s)	183, 512
GlaI	GCGC	2 cut(s)	506, 1401
GluI	GCNGC	6 cut(s)	157, 443, 494, 765, 1145, 1148
HaeIII	GGCC	4 cut(s)	156, 957, 1068, 1289
HapII	CCGG	2 cut(s)	408, 702
HgaI	GACGC	1 cut(s)	178
HhaI	GCGC	2 cut(s)	507, 1402
Hin1II	CATG	4 cut(s)	155, 551, 982, 1073
Hin6I	GCGC	2 cut(s)	505, 1400
HinP1I	GCGC	2 cut(s)	505, 1400
HincII	GTYRAC	2 cut(s)	193, 1300
HindII	GTYRAC	2 cut(s)	193, 1300
HindIII	AAGCTT	1 cut(s)	1157
HinfI	GANTC	6 cut(s)	25, 353, 842, 1124, 1430, 1442
HpaII	CCGG	2 cut(s)	408, 702
HphI	GGTGA	3 cut(s)	271, 637, 875
Hpy166II	GTNNAC	2 cut(s)	193, 1300
Hpy188III	TCNNGA	5 cut(s)	132, 776, 782, 914, 1181
Hpy8I	GTNNAC	2 cut(s)	193, 1300
Hpy99I	CGWCG	3 cut(s)	194, 197, 404
HpyAV	CCTTC	6 cut(s)	226, 249, 258, 463, 968, 1029
HpyCH4III	ACNGT	3 cut(s)	793, 901, 1105
HpyCH4IV	ACGT	1 cut(s)	1302
HpyCH4V	TGCA	5 cut(s)	658, 1144, 1358, 1363, 1418
HpyF10VI	GCNNNNNNNGC	7 cut(s)	156, 179, 212, 451, 502, 1156, 1241
HpyF3I	CTNAG	4 cut(s)	86, 610, 858, 1206
HpySE526I	ACGT	1 cut(s)	1302
Hsp92II	CATG	4 cut(s)	155, 551, 982, 1073
HspAI	GCGC	2 cut(s)	505, 1400
Kzo9I	GATC	8 cut(s)	134, 535, 555, 634, 681, 778, 962, 1183
LmnI	GCTCC	6 cut(s)	25, 70, 257, 734, 748, 880
Lsp1109I	GCAGC	4 cut(s)	454, 776, 1156, 1159
LweI	GCATC	4 cut(s)	746, 793, 907, 1231
MaeI	CTAG	2 cut(s)	183, 512
MaeII	ACGT	1 cut(s)	1302
MaeIII	GTNAC	2 cut(s)	613, 625
MalI	GATC	8 cut(s)	136, 537, 557, 636, 683, 780, 964, 1185
MbiI	CCGCTC	2 cut(s)	252, 1210
MboI	GATC	8 cut(s)	134, 535, 555, 634, 681, 778, 962, 1183
MflI	RGATCY	2 cut(s)	634, 1183
MhlI	GDGCHC	1 cut(s)	75
MlsI	TGGCCA	1 cut(s)	1068
MluCI	AATT	8 cut(s)	80, 529, 664, 1128, 1192, 1230, 1386, 1413
MluNI	TGGCCA	1 cut(s)	1068
MlyI	GAGTC	2 cut(s)	362, 1439
MmeI	TCCRAC	1 cut(s)	249
Mox20I	TGGCCA	1 cut(s)	1068
MscI	TGGCCA	1 cut(s)	1068
MseI	TTAA	5 cut(s)	447, 668, 834, 1227, 1343
MslI	CAYNNNNRTG	4 cut(s)	150, 623, 927, 1455
Msp20I	TGGCCA	1 cut(s)	1068
MspI	CCGG	2 cut(s)	408, 702
MspR9I	CCNGG	5 cut(s)	359, 409, 639, 999, 1113
MvaI	CCWGG	4 cut(s)	359, 639, 999, 1113
MvnI	CGCG	2 cut(s)	507, 1402
MwoI	GCNNNNNNNGC	7 cut(s)	156, 179, 212, 451, 502, 1156, 1241
NciI	CCSGG	1 cut(s)	409
NcoI	CCATGG	1 cut(s)	151
NdeII	GATC	8 cut(s)	134, 535, 555, 634, 681, 778, 962, 1183
NlaIII	CATG	4 cut(s)	155, 551, 982, 1073
NlaIV	GGNNCC	2 cut(s)	412, 882
NmeAIII	GCCGAG	1 cut(s)	577
NmuCI	GTSAC	1 cut(s)	625
NspV	TTCGAA	1 cut(s)	1445
OliI	CACNNNNGTG	2 cut(s)	927, 1455
PfeI	GAWTC	4 cut(s)	25, 842, 1124, 1442
PfoI	TCCNGGA	1 cut(s)	407
PkrI	GCNGC	6 cut(s)	158, 444, 495, 766, 1146, 1149
Ple19I	CGATCG	1 cut(s)	965
PleI	GAGTC	2 cut(s)	361, 1438
PpsI	GAGTC	2 cut(s)	361, 1438
PsiI	TTATAA	3 cut(s)	521, 1044, 1155
Psp6I	CCWGG	4 cut(s)	357, 637, 997, 1111
PspGI	CCWGG	4 cut(s)	357, 637, 997, 1111
PspN4I	GGNNCC	2 cut(s)	412, 882
PspPI	GGNCC	2 cut(s)	411, 1287
PsrI	GAACNNNNNNTAC	2 cut(s)	978, 1010
PstNI	CAGNNNCTG	1 cut(s)	797
PsuI	RGATCY	2 cut(s)	634, 1183
PvuI	CGATCG	1 cut(s)	965
RsaI	GTAC	3 cut(s)	1117, 1263, 1405
RsaNI	GTAC	3 cut(s)	1116, 1262, 1404
RseI	CAYNNNNRTG	4 cut(s)	150, 623, 927, 1455
SalI	GTCGAC	1 cut(s)	191
SaqAI	TTAA	5 cut(s)	447, 668, 834, 1227, 1343
SatI	GCNGC	6 cut(s)	157, 443, 494, 765, 1145, 1148
Sau3AI	GATC	8 cut(s)	134, 535, 555, 634, 681, 778, 962, 1183
Sau96I	GGNCC	2 cut(s)	411, 1287
SchI	GAGTC	2 cut(s)	362, 1439
ScrFI	CCNGG	5 cut(s)	359, 409, 639, 999, 1113
SduI	GDGCHC	1 cut(s)	75
SfaNI	GCATC	4 cut(s)	746, 793, 907, 1231
SfcI	CTRYAG	1 cut(s)	1475
SfuI	TTCGAA	1 cut(s)	1445
SgrDI	CGTCGACG	1 cut(s)	191
SinI	GGWCC	1 cut(s)	411
SmiMI	CAYNNNNRTG	4 cut(s)	150, 623, 927, 1455
SmlI	CTYRAG	3 cut(s)	715, 782, 1325
SmoI	CTYRAG	3 cut(s)	715, 782, 1325
SpeI	ACTAGT	1 cut(s)	511
Sse9I	AATT	8 cut(s)	80, 529, 664, 1128, 1192, 1230, 1386, 1413
SsiI	CCGC	7 cut(s)	157, 180, 250, 320, 494, 1073, 1210
SspMI	CTAG	2 cut(s)	183, 512
StyD4I	CCNGG	5 cut(s)	357, 407, 637, 997, 1111
StyI	CCWWGG	3 cut(s)	151, 255, 1290
TaaI	ACNGT	3 cut(s)	793, 901, 1105
TaiI	ACGT	1 cut(s)	1305
TaqI	TCGA	9 cut(s)	131, 192, 351, 438, 961, 1033, 1164, 1252, 1445
TaqII	GACCGA	1 cut(s)	428
TasI	AATT	8 cut(s)	80, 529, 664, 1128, 1192, 1230, 1386, 1413
TatI	WGTACW	1 cut(s)	1261
TauI	GCSGC	2 cut(s)	159, 496
TfiI	GAWTC	4 cut(s)	25, 842, 1124, 1442
Tru1I	TTAA	5 cut(s)	447, 668, 834, 1227, 1343
Tru9I	TTAA	5 cut(s)	447, 668, 834, 1227, 1343
TscAI	CASTG	2 cut(s)	929, 1425
TseFI	GTSAC	1 cut(s)	625
TseI	GCWGC	4 cut(s)	442, 764, 1144, 1147
Tsp45I	GTSAC	1 cut(s)	625
TspDTI	ATGAA	6 cut(s)	309, 482, 759, 957, 1137, 1160
TspGWI	ACGGA	2 cut(s)	840, 1445
TspRI	CASTG	2 cut(s)	929, 1425
VpaK11BI	GGWCC	1 cut(s)	411
XagI	CCTNNNNNAGG	1 cut(s)	718
XapI	RAATTY	3 cut(s)	529, 664, 1386
XmiI	GTMKAC	1 cut(s)	192
XspI	CTAG	2 cut(s)	183, 512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.