Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0009864

Physical Location & Seq

Genomic Coordinates Reverse (-)

191728 .. 197213

Gene Structure 5486 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g00350.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1533 bp

ATGGCCGGCGTTACAGTCCAGATCACTACTAGTACCGGATGCGCAGACGTCTACTCATCATTTGACGACTCTCTGATGGCGACTATCTCATCAGCTCCTCCTCCTCGTCTTCCCTCTCTGTCTTCCTCTTCGCGCCTCTGCTTTGCCTCCTCCGAAACTCTCGCGTTTCCTCCTTCAGCTTCGTCATCAGGTTCGCGATCCATAAGTCACACCCACCTCTTCAACAAGCTCATCGTCTCCGCTTCTTCTAGAAGCATCCACCGAACCGTCCCCGATAACAACGGCTCTCTCCCTCCTTCAACCCCCAAGGGAGTGAGGAGGCATGCAATCTCAGTGTTCGTCGGGGACGAAAGCGGAATGATCAATCGGATTTCAGGGGTGTTTGCAAGGAGGGGATACAACATCGAGTCACTTGCTGTTGGCTTGAACAAGGACAAAGCCCTCTTCACCATTGTCGTTTCTGGCACCGATACCGTCTTACGCCAAGTCATTGAGCAGCTTCACAAGCTCGTCAATGTTCTCAAGGTTGAGGATCTCTCTAGTGAACCTCAAGTAGAACGTGAATTAATGCTTATCAAAGTTACTGCACATCCAGACTACCTTGCTGAGATCAGGTGGATAGTGAATATCTTTAGAGCTAAAATCGTGGATATCTCAGACCACTCAGTAACCATTGAGGTGACAGGAGATCCTGGGAAGATGGTTGCTCTTCAAAGAAATTTAAGCAAGTTTGGGATCAGGGAAATTGCCAGAACCGGAAAGATAGCTTTGAGGAGAGAAAAGTTGGGTGCTTCTGCTCCATTTTGGCGCTATTCAGCAGCTTCATATCCAGATCTTGAGAGAAAAGTGACTGTTGATGCTCTTGCAGGGGCTGAGAATGGCGTTGTCAATCCCGAGTCTGATACATCTTCTGGGGGTGATGTTTATCCTGTGGAGCCTTCTGGTACCGCTAGTGTCAATCAAATCCTTAATGCTAACTGGGGTGTTCTCAATGATGAAGATACAAGTGGACTTCGATCACACACTTTATCCATGCTTGTAAATGATGTTCCTGGGGTTCTTTACATTATTACGGGCGTTTTTGCCAGAAGGGGTTATAACATTCAGAGTTTGGCTGTCGGCCGTGCTGAAGTTGAGGGGCAATCTCGCATTACTACTGTTGTTCCTGCTACAGATGAATCAATTAGCAAGTTGGTGCAACAGCTTTATAAGCTTGTTGATGTACATGATGTTCGAGATCTTACCAACTTGCCATTTGCTGAGCGGGAATTGATGTTGATAAAAATAGCTGCCAATGCTGTTGCTCGGAGAGACGTACTTGACATTGGAAACATCTTTAGGGCCAAGGCGGTTGACGTTTCTGACCACACTATAACCCTTGAGCTCACTGGAGACCTGAACAAGATGGTTGCACTGCAAAGATTGTTAGAACCCTATGGGATTTGTGAGGTTGCGCGTACTGGAAGAATTGCTCTGGTGCGTGAGTCAGGGGTCGATTCTAGATATCTCCGTGGATATTCTCTTCCTCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0005948 GO:0006082 GO:0006520 GO:0006549 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009097 GO:0009099 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0032991 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043621 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0050790 GO:0065007 GO:0065009 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

ACT ACT_5 ALS_ss_C

Protein Analysis

511

Amino Acids

55.33

Weight (kDa)

7.17

Isoelectric Point (pI)

43.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACT	PF01842	108 - 171	1.2e-09	ACT domain
ACT_AHAS_ss	PF22629	110 - 175	1.8e-16	AHAS small subunit-like ACT domain
ACT_5	PF13710	118 - 176	2.4e-07	ACT domain
ALS_ss_C	PF10369	187 - 260	2.3e-27	Small subunit of acetolactate synthase
ACT	PF01842	341 - 405	4.5e-09	ACT domain
ACT_AHAS_ss	PF22629	343 - 409	3.9e-21	AHAS small subunit-like ACT domain
ACT_5	PF13710	350 - 411	3.4e-10	ACT domain
ALS_ss_C	PF10369	422 - 494	2.8e-26	Small subunit of acetolactate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009864)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31810 AT2G31810 AT2G31810
fragaria_vesca	FvH4_1g00350 FvH4_1g00350
malus_domestica	MD02G1002700.v1.1 MD15G1145900.v1.1
prunus_persica	Prupe.7G268500_v2.0.a1
pyrus_communis	pycom15g13110
rosa_chinensis	RchiOBHm_Chr2g0084781
rosa_laevigata	RLG00000015638
rosa_multiflora	Rmu_sc0002352.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155820
rosa_rugosa	Rorug01G0456000 Rorug01G0456100 Rorug01G0456200
rosa_samantha	Rh2AG003300 Rh2BG004900 Rh2CG005300 Rh2DG004700
rosa_wichuraiana	Rw2G000380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1098, 1209
AatII	GACGTC	1 cut(s)	51
Acc16I	TGCGCA	1 cut(s)	43
Acc65I	GGTACC	1 cut(s)	944
AccB1I	GGYRCC	2 cut(s)	464, 944
AccBSI	CCGCTC	1 cut(s)	1264
AccI	GTMKAC	1 cut(s)	51
AccII	CGCG	4 cut(s)	133, 164, 196, 1456
AciI	CCGC	5 cut(s)	240, 354, 948, 1264, 1349
AclWI	GGATC	4 cut(s)	192, 540, 683, 743
AcoI	YGGCCR	2 cut(s)	3, 1120
AcsI	RAATTY	1 cut(s)	718
AcuI	CTGAAG	2 cut(s)	159, 1149
AcyI	GRCGYC	1 cut(s)	48
AfaI	GTAC	5 cut(s)	34, 946, 1224, 1317, 1459
AgsI	TTSAA	4 cut(s)	223, 300, 427, 713
AhlI	ACTAGT	1 cut(s)	29
AjnI	CCWGG	2 cut(s)	691, 1051
Alw21I	GWGCWC	1 cut(s)	1386
Alw26I	GTCTC	3 cut(s)	241, 1305, 1386
AlwI	GGATC	4 cut(s)	192, 540, 683, 743
AlwNI	CAGNNNCTG	1 cut(s)	872
Ama87I	CYCGRG	1 cut(s)	893
AoxI	GGCC	3 cut(s)	3, 1120, 1341
ApeKI	GCWGC	3 cut(s)	496, 818, 1289
ApoI	RAATTY	1 cut(s)	718
AseI	ATTAAT	1 cut(s)	566
Asp718I	GGTACC	1 cut(s)	944
AspLEI	GCGC	4 cut(s)	44, 135, 810, 1456
AspS9I	GGNCC	1 cut(s)	1341
AsuHPI	GGTGA	3 cut(s)	439, 691, 929
AvaI	CYCGRG	1 cut(s)	893
BanI	GGYRCC	2 cut(s)	464, 944
BanII	GRGCYC	1 cut(s)	1386
BbsI	GAAGAC	2 cut(s)	101, 114
Bbv12I	GWGCWC	1 cut(s)	1386
BbvI	GCAGC	3 cut(s)	508, 830, 1276
BccI	CCATC	3 cut(s)	70, 694, 1399
BceAI	ACGGC	2 cut(s)	298, 1107
BcgI	CGANNNNNNTGC	2 cut(s)	395, 429
BciT130I	CCWGG	2 cut(s)	693, 1053
BciVI	GTATCC	1 cut(s)	389
BclI	TGATCA	1 cut(s)	360
BcoDI	GTCTC	3 cut(s)	241, 1305, 1386
BcuI	ACTAGT	1 cut(s)	29
BfaI	CTAG	5 cut(s)	30, 249, 540, 951, 1500
BfmI	CTRYAG	1 cut(s)	1170
BfoI	RGCGCY	1 cut(s)	811
BfuI	GTATCC	1 cut(s)	389
BglII	AGATCT	2 cut(s)	832, 1237
BisI	GCNGC	3 cut(s)	497, 819, 1290
BlpI	GCTNAGC	1 cut(s)	1260
BlsI	GCNGC	3 cut(s)	498, 820, 1291
Bme1390I	CCNGG	2 cut(s)	693, 1053
BmeT110I	CYCGRG	1 cut(s)	893
BmgT120I	GGNCC	1 cut(s)	1341
BmiI	GGNNCC	3 cut(s)	466, 936, 946
BmrFI	CCNGG	2 cut(s)	693, 1053
BmrI	ACTGGG	1 cut(s)	988
BmsI	GCATC	3 cut(s)	29, 264, 847
BmuI	ACTGGG	1 cut(s)	988
BpiI	GAAGAC	2 cut(s)	101, 114
BplI	GAGNNNNNCTC	2 cut(s)	521, 553
BpmI	CTGGAG	1 cut(s)	1410
Bpu1102I	GCTNAGC	1 cut(s)	1260
BpuEI	CTTGAG	4 cut(s)	506, 534, 857, 1400
BsaBI	GATNNNNATC	1 cut(s)	924
BsaHI	GRCGYC	1 cut(s)	48
BsaI	GGTCTC	1 cut(s)	1386
BsaJI	CCNNGG	5 cut(s)	306, 692, 1052, 1344, 1510
BsaWI	WCCGGW	2 cut(s)	35, 755
Bse118I	RCCGGY	1 cut(s)	5
Bse1I	ACTGG	3 cut(s)	983, 1393, 1465
Bse8I	GATNNNNATC	1 cut(s)	924
BseBI	CCWGG	2 cut(s)	693, 1053
BseDI	CCNNGG	5 cut(s)	306, 692, 1052, 1344, 1510
BseGI	GGATG	3 cut(s)	44, 255, 589
BseJI	GATNNNNATC	1 cut(s)	924
BseMII	CTCAG	6 cut(s)	345, 597, 669, 678, 864, 1251
BseNI	ACTGG	3 cut(s)	983, 1393, 1465
BseRI	GAGGAG	6 cut(s)	87, 90, 93, 139, 331, 787
BseX3I	CGGCCG	1 cut(s)	1120
BseXI	GCAGC	3 cut(s)	508, 830, 1276
BsgI	GTGCAG	1 cut(s)	570
Bsh1236I	CGCG	4 cut(s)	133, 164, 196, 1456
Bsh1285I	CGRYCG	1 cut(s)	1123
BshFI	GGCC	3 cut(s)	5, 1122, 1343
BshNI	GGYRCC	2 cut(s)	464, 944
BsiEI	CGRYCG	1 cut(s)	1123
BsiHKAI	GWGCWC	1 cut(s)	1386
BsiHKCI	CYCGRG	1 cut(s)	893
BsiSI	CCGG	3 cut(s)	6, 36, 756
BslFI	GGGAC	2 cut(s)	254, 359
BsmAI	GTCTC	3 cut(s)	241, 1305, 1386
BsmBI	CGTCTC	2 cut(s)	241, 1305
BsmFI	GGGAC	2 cut(s)	254, 359
BsnI	GGCC	3 cut(s)	5, 1122, 1343
Bso31I	GGTCTC	1 cut(s)	1386
BsoBI	CYCGRG	1 cut(s)	893
Bsp1286I	GDGCHC	1 cut(s)	1386
Bsp1407I	TGTACA	1 cut(s)	1222
Bsp1720I	GCTNAGC	1 cut(s)	1260
Bsp68I	TCGCGA	1 cut(s)	196
BspACI	CCGC	5 cut(s)	240, 354, 948, 1264, 1349
BspANI	GGCC	3 cut(s)	5, 1122, 1343
BspCNI	CTCAG	6 cut(s)	344, 598, 668, 677, 865, 1252
BspFNI	CGCG	4 cut(s)	133, 164, 196, 1456
BspLI	GGNNCC	3 cut(s)	466, 936, 946
BspPI	GGATC	4 cut(s)	192, 540, 683, 743
BspQI	GCTCTTC	1 cut(s)	714
BspT107I	GGYRCC	2 cut(s)	464, 944
BspTNI	GGTCTC	1 cut(s)	1386
BsrBI	CCGCTC	1 cut(s)	1264
BsrFI	RCCGGY	1 cut(s)	5
BsrGI	TGTACA	1 cut(s)	1222
BsrI	ACTGG	3 cut(s)	983, 1393, 1465
BssAI	RCCGGY	1 cut(s)	5
BssECI	CCNNGG	5 cut(s)	306, 692, 1052, 1344, 1510
BssNI	GRCGYC	1 cut(s)	48
BssT1I	CCWWGG	2 cut(s)	306, 1344
Bst2UI	CCWGG	2 cut(s)	693, 1053
Bst4CI	ACNGT	5 cut(s)	16, 268, 475, 853, 1159
Bst6I	CTCTTC	5 cut(s)	133, 224, 449, 714, 1527
BstACI	GRCGYC	1 cut(s)	48
BstAUI	TGTACA	1 cut(s)	1222
BstC8I	GCNNGC	2 cut(s)	7, 324
BstDEI	CTNAG	6 cut(s)	331, 606, 655, 664, 873, 1260
BstDSI	CCRYGG	1 cut(s)	1510
BstF5I	GGATG	3 cut(s)	44, 255, 589
BstFNI	CGCG	4 cut(s)	133, 164, 196, 1456
BstH2I	RGCGCY	1 cut(s)	811
BstHHI	GCGC	4 cut(s)	44, 135, 810, 1456
BstMAI	GTCTC	3 cut(s)	241, 1305, 1386
BstMCI	CGRYCG	1 cut(s)	1123
BstMWI	GCNNNNNNNGC	3 cut(s)	505, 1210, 1295
BstNI	CCWGG	2 cut(s)	693, 1053
BstNSI	RCATGY	1 cut(s)	326
BstSCI	CCNGG	2 cut(s)	691, 1051
BstSFI	CTRYAG	1 cut(s)	1170
BstUI	CGCG	4 cut(s)	133, 164, 196, 1456
BstV1I	GCAGC	3 cut(s)	508, 830, 1276
BstV2I	GAAGAC	2 cut(s)	101, 114
BstX2I	RGATCY	4 cut(s)	532, 688, 832, 1237
BstYI	RGATCY	4 cut(s)	532, 688, 832, 1237
BstZI	CGGCCG	1 cut(s)	1120
BsuI	GTATCC	1 cut(s)	389
BsuRI	GGCC	3 cut(s)	5, 1122, 1343
BtgI	CCRYGG	1 cut(s)	1510
BtsCI	GGATG	3 cut(s)	44, 255, 589
BtsI	GCAGTG	1 cut(s)	1412
BtsIMutI	CAGTG	3 cut(s)	339, 1386, 1412
BtuMI	TCGCGA	1 cut(s)	196
Cac8I	GCNNGC	2 cut(s)	7, 324
CaiI	CAGNNNCTG	1 cut(s)	872
CfoI	GCGC	4 cut(s)	44, 135, 810, 1456
Cfr10I	RCCGGY	1 cut(s)	5
Cfr13I	GGNCC	1 cut(s)	1341
Csp6I	GTAC	5 cut(s)	33, 945, 1223, 1316, 1458
CviAII	CATG	3 cut(s)	323, 1033, 1226
CviQI	GTAC	5 cut(s)	33, 945, 1223, 1316, 1458
DdeI	CTNAG	6 cut(s)	331, 606, 655, 664, 873, 1260
EaeI	YGGCCR	2 cut(s)	3, 1120
EagI	CGGCCG	1 cut(s)	1120
Eam1104I	CTCTTC	5 cut(s)	133, 224, 449, 714, 1527
EarI	CTCTTC	5 cut(s)	133, 224, 449, 714, 1527
Ecl136II	GAGCTC	1 cut(s)	1384
EclXI	CGGCCG	1 cut(s)	1120
Eco130I	CCWWGG	2 cut(s)	306, 1344
Eco24I	GRGCYC	1 cut(s)	1386
Eco31I	GGTCTC	1 cut(s)	1386
Eco32I	GATATC	2 cut(s)	652, 1505
Eco52I	CGGCCG	1 cut(s)	1120
Eco53kI	GAGCTC	1 cut(s)	1384
Eco57I	CTGAAG	2 cut(s)	159, 1149
Eco88I	CYCGRG	1 cut(s)	893
EcoICRI	GAGCTC	1 cut(s)	1384
EcoRII	CCWGG	2 cut(s)	691, 1051
EcoRV	GATATC	2 cut(s)	652, 1505
EcoT14I	CCWWGG	2 cut(s)	306, 1344
EcoT38I	GRGCYC	1 cut(s)	1386
ErhI	CCWWGG	2 cut(s)	306, 1344
Esp3I	CGTCTC	2 cut(s)	241, 1305
FaeI	CATG	3 cut(s)	326, 1036, 1229
FaiI	YATR	9 cut(s)	203, 324, 826, 1034, 1098, 1209, 1227, 1373, 1437
FaqI	GGGAC	2 cut(s)	254, 359
FatI	CATG	3 cut(s)	322, 1032, 1225
FauI	CCCGC	1 cut(s)	1257
FbaI	TGATCA	1 cut(s)	360
FblI	GTMKAC	1 cut(s)	51
Fnu4HI	GCNGC	3 cut(s)	497, 819, 1290
FokI	GGATG	3 cut(s)	51, 242, 576
FriOI	GRGCYC	1 cut(s)	1386
Fsp4HI	GCNGC	3 cut(s)	497, 819, 1290
FspBI	CTAG	5 cut(s)	30, 249, 540, 951, 1500
FspI	TGCGCA	1 cut(s)	43
GlaI	GCGC	4 cut(s)	43, 134, 809, 1455
GluI	GCNGC	3 cut(s)	497, 819, 1290
GsuI	CTGGAG	1 cut(s)	1410
HaeII	RGCGCY	1 cut(s)	811
HaeIII	GGCC	3 cut(s)	5, 1122, 1343
HapII	CCGG	3 cut(s)	6, 36, 756
HhaI	GCGC	4 cut(s)	44, 135, 810, 1456
Hin1I	GRCGYC	1 cut(s)	48
Hin1II	CATG	3 cut(s)	326, 1036, 1229
Hin6I	GCGC	4 cut(s)	42, 133, 808, 1454
HinP1I	GCGC	4 cut(s)	42, 133, 808, 1454
HincII	GTYRAC	1 cut(s)	1354
HindII	GTYRAC	1 cut(s)	1354
HindIII	AAGCTT	1 cut(s)	1211
HinfI	GANTC	6 cut(s)	68, 407, 896, 1178, 1484, 1496
HpaII	CCGG	3 cut(s)	6, 36, 756
HphI	GGTGA	3 cut(s)	439, 691, 929
Hpy166II	GTNNAC	4 cut(s)	52, 545, 1010, 1354
Hpy188I	TCNGA	8 cut(s)	75, 154, 369, 658, 901, 1107, 1308, 1363
Hpy188III	TCNNGA	9 cut(s)	19, 195, 249, 593, 830, 836, 893, 1235, 1500
Hpy8I	GTNNAC	4 cut(s)	52, 545, 1010, 1354
Hpy99I	CGWCG	1 cut(s)	344
HpyAV	CCTTC	4 cut(s)	183, 306, 948, 1083
HpyCH4III	ACNGT	5 cut(s)	16, 268, 475, 853, 1159
HpyCH4IV	ACGT	4 cut(s)	48, 559, 1314, 1356
HpyCH4V	TGCA	7 cut(s)	326, 386, 587, 866, 1198, 1412, 1417
HpyF10VI	GCNNNNNNNGC	3 cut(s)	505, 1210, 1295
HpyF3I	CTNAG	6 cut(s)	331, 606, 655, 664, 873, 1260
HpySE526I	ACGT	4 cut(s)	48, 559, 1314, 1356
Hsp92I	GRCGYC	1 cut(s)	48
Hsp92II	CATG	3 cut(s)	326, 1036, 1229
HspAI	GCGC	4 cut(s)	42, 133, 808, 1454
KpnI	GGTACC	1 cut(s)	948
KroI	GCCGGC	1 cut(s)	5
KroNI	GCCGGC	1 cut(s)	7
Ksp22I	TGATCA	1 cut(s)	360
LguI	GCTCTTC	1 cut(s)	714
LmnI	GCTCC	3 cut(s)	100, 802, 934
Lsp1109I	GCAGC	3 cut(s)	508, 830, 1276
LweI	GCATC	3 cut(s)	29, 264, 847
MaeI	CTAG	5 cut(s)	30, 249, 540, 951, 1500
MaeII	ACGT	4 cut(s)	48, 559, 1314, 1356
MaeIII	GTNAC	7 cut(s)	10, 206, 408, 580, 667, 679, 847
MbiI	CCGCTC	1 cut(s)	1264
MflI	RGATCY	4 cut(s)	532, 688, 832, 1237
MhlI	GDGCHC	1 cut(s)	1386
MluCI	AATT	6 cut(s)	563, 718, 744, 1182, 1268, 1467
MlyI	GAGTC	4 cut(s)	62, 416, 905, 1493
MroNI	GCCGGC	1 cut(s)	5
MseI	TTAA	3 cut(s)	566, 722, 969
MslI	CAYNNNNRTG	1 cut(s)	677
MspI	CCGG	3 cut(s)	6, 36, 756
MspR9I	CCNGG	2 cut(s)	693, 1053
MvaI	CCWGG	2 cut(s)	693, 1053
MvnI	CGCG	4 cut(s)	133, 164, 196, 1456
MwoI	GCNNNNNNNGC	3 cut(s)	505, 1210, 1295
NaeI	GCCGGC	1 cut(s)	7
NgoMIV	GCCGGC	1 cut(s)	5
NlaIII	CATG	3 cut(s)	326, 1036, 1229
NlaIV	GGNNCC	3 cut(s)	466, 936, 946
NmuCI	GTSAC	4 cut(s)	206, 408, 679, 847
NruI	TCGCGA	1 cut(s)	196
NsbI	TGCGCA	1 cut(s)	43
NspI	RCATGY	1 cut(s)	326
PaeI	GCATGC	1 cut(s)	326
PciSI	GCTCTTC	1 cut(s)	714
PcsI	WCGNNNNNNNCGW	1 cut(s)	345
PdiI	GCCGGC	1 cut(s)	7
PfeI	GAWTC	2 cut(s)	1178, 1496
PkrI	GCNGC	3 cut(s)	498, 820, 1291
PleI	GAGTC	4 cut(s)	62, 415, 904, 1492
PpsI	GAGTC	4 cut(s)	62, 415, 904, 1492
PshBI	ATTAAT	1 cut(s)	566
PsiI	TTATAA	2 cut(s)	1098, 1209
Psp124BI	GAGCTC	1 cut(s)	1386
Psp6I	CCWGG	2 cut(s)	691, 1051
PspGI	CCWGG	2 cut(s)	691, 1051
PspN4I	GGNNCC	3 cut(s)	466, 936, 946
PspPI	GGNCC	1 cut(s)	1341
PsrI	GAACNNNNNNTAC	4 cut(s)	1032, 1064, 1215, 1247
PstNI	CAGNNNCTG	1 cut(s)	872
PsuI	RGATCY	4 cut(s)	532, 688, 832, 1237
RruI	TCGCGA	1 cut(s)	196
RsaI	GTAC	5 cut(s)	34, 946, 1224, 1317, 1459
RsaNI	GTAC	5 cut(s)	33, 945, 1223, 1316, 1458
RseI	CAYNNNNRTG	1 cut(s)	677
SacI	GAGCTC	1 cut(s)	1386
SapI	GCTCTTC	1 cut(s)	714
SaqAI	TTAA	3 cut(s)	566, 722, 969
SatI	GCNGC	3 cut(s)	497, 819, 1290
Sau96I	GGNCC	1 cut(s)	1341
SchI	GAGTC	4 cut(s)	62, 416, 905, 1493
ScrFI	CCNGG	2 cut(s)	693, 1053
SduI	GDGCHC	1 cut(s)	1386
SfaNI	GCATC	3 cut(s)	29, 264, 847
SfcI	CTRYAG	1 cut(s)	1170
SmiMI	CAYNNNNRTG	1 cut(s)	677
SmlI	CTYRAG	4 cut(s)	521, 549, 836, 1379
SmoI	CTYRAG	4 cut(s)	521, 549, 836, 1379
SpeI	ACTAGT	1 cut(s)	29
SphI	GCATGC	1 cut(s)	326
Sse9I	AATT	6 cut(s)	563, 718, 744, 1182, 1268, 1467
SsiI	CCGC	5 cut(s)	240, 354, 948, 1264, 1349
SspMI	CTAG	5 cut(s)	30, 249, 540, 951, 1500
SstI	GAGCTC	1 cut(s)	1386
StyD4I	CCNGG	2 cut(s)	691, 1051
StyI	CCWWGG	2 cut(s)	306, 1344
TaaI	ACNGT	5 cut(s)	16, 268, 475, 853, 1159
TaiI	ACGT	4 cut(s)	51, 562, 1317, 1359
TaqI	TCGA	4 cut(s)	405, 1015, 1234, 1494
TasI	AATT	6 cut(s)	563, 718, 744, 1182, 1268, 1467
TatI	WGTACW	1 cut(s)	1222
TfiI	GAWTC	2 cut(s)	1178, 1496
Tru1I	TTAA	3 cut(s)	566, 722, 969
Tru9I	TTAA	3 cut(s)	566, 722, 969
TscAI	CASTG	3 cut(s)	339, 1393, 1419
TseFI	GTSAC	4 cut(s)	206, 408, 679, 847
TseI	GCWGC	3 cut(s)	496, 818, 1289
Tsp45I	GTSAC	4 cut(s)	206, 408, 679, 847
TspDTI	ATGAA	3 cut(s)	813, 1011, 1191
TspGWI	ACGGA	1 cut(s)	1499
TspRI	CASTG	3 cut(s)	339, 1393, 1419
VspI	ATTAAT	1 cut(s)	566
XapI	RAATTY	1 cut(s)	718
XbaI	TCTAGA	2 cut(s)	248, 1499
XceI	RCATGY	1 cut(s)	326
XmiI	GTMKAC	1 cut(s)	51
XspI	CTAG	5 cut(s)	30, 249, 540, 951, 1500
ZraI	GACGTC	1 cut(s)	49

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.