Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0009864

Physical Location & Seq

Genomic Coordinates Reverse (-)

217806 .. 222449

Gene Structure 4644 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1002700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1470 bp

ATGGCGACCATTTCTTCAGCTTCAGTTCGCACTCCGAACTTCTCTTGGTCCTTCTCCTCGGAGCACCTCCAATCCCTCGGGTTCTCCGAAACTCTATTGTTTCCTCTGACGAGTTCAGCTTCGAGATCAATAATCCATAGCCATGACCGCCACAAGCAACTCGCAGTCTCCGCTAGAAGCTTCGACCAGAATATCGCCGACAGCGCCTTCTCTTCCAACGGCTCTCCTCCTTCGACCACCCGCTCCAAGGTAAGAAGGCACACGATTTCGGTGTTCGTTGGGGACGAAAGTGGAATGATAAATCGGATAGCAGGGGTGTTTGCTAGGAGGGGATACAACATCGAGTCCCTGGCTGTTGGTCTGAACAAGGACAAAGCTCTCTTCACCATCGTCGTCTCCGGAACCGAAACGGTCTTACGCCTCGTGATCGAGCAGCTTAACAAGCTCGTCAATGTTATGAAGGTTGAAGATCTCTCAATTGAGCCTCAGGTGGAGCGTGAACTAATGCTTATAAAAGTAAATGCCGATCCTAGCTATCATGCCGAGATCAAGTGGTTAGTGAATATCTTTAGAGCAAAGATTGTGGATATCTCGGAACACTCAGTAACCATTGAGGTGACAGGAGATCCTGGGAAGATGGTTGCTCTGCAAAGAAATTTAAGCAAGTTTGGGATCAGAGAAATTGCCAGAACCGGAAAGATTGCCTTGAGGAGGGAAAAGTTGGGTGCATCCGCTCCATTTTGGCGATATTCAGCAGCTTCATATCCTGACCTTGAGAGAACAGTTCCTGTTGATGCTCTTGTGGGGGTTGAAAACGGACTAGTTAATGCTGTATCTGATGTGTCTTCTGGGGGTGATGTTTATCCTGTGGAGCCGTCTGATACCTTTACTGTCAATCAAGTTCTTGATGCTCACTGGGGTGTTCTCGATGACGAAGATACTAATGGACTTCGATCACACACTTTATCCATGCTCGTGAATGACATTCCTGGGGTTCTCTACATTATTACTGGCGTTTTTGCCCGAAGGGGTTATAACATTCAGAGCTTAGCTGTTGGCCGTGCAGAAGCTGAGGGGCTATCTCGCATTACTACTGTTGTTCCTGGTACAGATGAATCCATTAGCAAGTTGGTGCAGCAGCTTTATAAGCTTGTCGATGTTCATGATGTTCAAGATCTTACCAATTTGCCATTTGCCGAGCGGGAATTGATGTTGATAAAAATTGCTGCGAATGCTGCTGCTCGAAGAGATGTGCTTGATATTGGCAACATTTTTAGGGCCAAGGCTGTTGACGTTTCTGATCACACAATAACCCTTGAGCTAACAGGAGACCTGGACAAGATGGTTGCATTGCAGAGGTTGTTAGAGCCCTATGGAATTTGTGAGGTGGCGCGTACTGGAAGAATTGCACTGGTGCGTGAGTCGGGTGTGGATTCGAAATACCTCCGTGGATATTCTTTTCCTCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0005948 GO:0006082 GO:0006520 GO:0006549 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009097 GO:0009099 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0032991 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043621 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0050790 GO:0065007 GO:0065009 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

ACT ACT_5 ALS_ss_C

Protein Analysis

490

Amino Acids

53.69

Weight (kDa)

6.16

Isoelectric Point (pI)

42.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACT	PF01842	87 - 150	9.6e-10	ACT domain
ACT_AHAS_ss	PF22629	89 - 154	4.6e-15	AHAS small subunit-like ACT domain
ACT_5	PF13710	97 - 155	3.2e-08	ACT domain
ALS_ss_C	PF10369	166 - 239	2.2e-28	Small subunit of acetolactate synthase
ACT	PF01842	320 - 385	2.8e-09	ACT domain
ACT_AHAS_ss	PF22629	322 - 388	1.2e-21	AHAS small subunit-like ACT domain
ACT_5	PF13710	330 - 390	8.1e-11	ACT domain
ALS_ss_C	PF10369	401 - 473	6.7e-27	Small subunit of acetolactate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009864)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31810 AT2G31810 AT2G31810
fragaria_vesca	FvH4_1g00350 FvH4_1g00350
malus_domestica	MD02G1002700.v1.1 MD15G1145900.v1.1
prunus_persica	Prupe.7G268500_v2.0.a1
pyrus_communis	pycom15g13110
rosa_chinensis	RchiOBHm_Chr2g0084781
rosa_laevigata	RLG00000015638
rosa_multiflora	Rmu_sc0002352.1_g000002
rosa_roxburghii	Rroxscaffold_2G00155820
rosa_rugosa	Rorug01G0456000 Rorug01G0456100 Rorug01G0456200
rosa_samantha	Rh2AG003300 Rh2BG004900 Rh2CG005300 Rh2DG004700
rosa_wichuraiana	Rw2G000380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	512, 1035, 1146
AccBSI	CCGCTC	3 cut(s)	243, 734, 1201
AccII	CGCG	1 cut(s)	1393
AccIII	TCCGGA	1 cut(s)	398
AciI	CCGC	5 cut(s)	148, 171, 241, 732, 1201
AclWI	GGATC	3 cut(s)	521, 620, 680
AcoI	YGGCCR	1 cut(s)	1057
AcsI	RAATTY	2 cut(s)	655, 1377
AcuI	CTGAAG	1 cut(s)	6
AfaI	GTAC	2 cut(s)	1108, 1396
AfiI	CCNNNNNNNGG	2 cut(s)	247, 711
AgsI	TTSAA	3 cut(s)	467, 812, 1172
AhlI	ACTAGT	1 cut(s)	820
AjnI	CCWGG	5 cut(s)	348, 628, 988, 1102, 1332
AleI	CACNNNNGTG	1 cut(s)	918
Alw21I	GWGCWC	1 cut(s)	66
Alw26I	GTCTC	3 cut(s)	172, 400, 1323
AlwI	GGATC	3 cut(s)	521, 620, 680
AlwNI	CAGNNNCTG	2 cut(s)	788, 1070
Ama87I	CYCGRG	1 cut(s)	77
Aor13HI	TCCGGA	1 cut(s)	398
AoxI	GGCC	2 cut(s)	1057, 1278
ApeKI	GCWGC	7 cut(s)	433, 755, 1135, 1138, 1226, 1235, 1238
ApoI	RAATTY	2 cut(s)	655, 1377
AspLEI	GCGC	2 cut(s)	206, 1393
AspS9I	GGNCC	2 cut(s)	48, 1278
AsuHPI	GGTGA	3 cut(s)	376, 628, 866
AsuII	TTCGAA	1 cut(s)	1436
AvaI	CYCGRG	1 cut(s)	77
AvaII	GGWCC	1 cut(s)	48
AxyI	CCTNAGG	1 cut(s)	486
BanII	GRGCYC	1 cut(s)	1371
BauI	CACGAG	2 cut(s)	422, 974
BbsI	GAAGAC	1 cut(s)	837
Bbv12I	GWGCWC	1 cut(s)	66
BbvCI	CCTCAGC	1 cut(s)	1071
BbvI	GCAGC	7 cut(s)	445, 767, 1147, 1150, 1213, 1222, 1225
BccI	CCATC	3 cut(s)	395, 631, 1336
BceAI	ACGGC	3 cut(s)	235, 859, 1044
BciT130I	CCWGG	5 cut(s)	350, 630, 990, 1104, 1334
BciVI	GTATCC	1 cut(s)	326
BclI	TGATCA	1 cut(s)	1300
BcoDI	GTCTC	3 cut(s)	172, 400, 1323
BcuI	ACTAGT	1 cut(s)	820
BfaI	CTAG	4 cut(s)	174, 324, 531, 821
BfmI	CTRYAG	1 cut(s)	1466
BfoI	RGCGCY	1 cut(s)	207
BfuI	GTATCC	1 cut(s)	326
BglII	AGATCT	2 cut(s)	469, 1174
BisI	GCNGC	7 cut(s)	434, 756, 1136, 1139, 1227, 1236, 1239
BlpI	GCTNAGC	1 cut(s)	1048
BlsI	GCNGC	7 cut(s)	435, 757, 1137, 1140, 1228, 1237, 1240
Bme1390I	CCNGG	5 cut(s)	350, 630, 990, 1104, 1334
Bme18I	GGWCC	1 cut(s)	48
BmeT110I	CYCGRG	1 cut(s)	77
BmgT120I	GGNCC	2 cut(s)	48, 1278
BmiI	GGNNCC	2 cut(s)	403, 873
BmrFI	CCNGG	5 cut(s)	350, 630, 990, 1104, 1334
BmrI	ACTGGG	1 cut(s)	925
BmsI	GCATC	3 cut(s)	737, 784, 898
BmuI	ACTGGG	1 cut(s)	925
BpiI	GAAGAC	1 cut(s)	837
Bpu10I	CCTNAGC	1 cut(s)	1071
Bpu1102I	GCTNAGC	1 cut(s)	1048
Bpu14I	TTCGAA	1 cut(s)	1436
BpuEI	CTTGAG	3 cut(s)	727, 794, 1337
BsaBI	GATNNNNATC	1 cut(s)	861
BsaI	GGTCTC	1 cut(s)	1323
BsaJI	CCNNGG	8 cut(s)	57, 76, 246, 348, 629, 989, 1281, 1447
BsaWI	WCCGGW	2 cut(s)	398, 692
Bsc4I	CCNNNNNNNGG	2 cut(s)	247, 711
Bse1I	ACTGG	4 cut(s)	920, 1015, 1402, 1416
Bse21I	CCTNAGG	1 cut(s)	486
Bse3DI	GCAATG	1 cut(s)	1349
Bse8I	GATNNNNATC	1 cut(s)	861
BseAI	TCCGGA	1 cut(s)	398
BseBI	CCWGG	5 cut(s)	350, 630, 990, 1104, 1334
BseDI	CCNNGG	8 cut(s)	57, 76, 246, 348, 629, 989, 1281, 1447
BseGI	GGATG	1 cut(s)	728
BseJI	GATNNNNATC	1 cut(s)	861
BseLI	CCNNNNNNNGG	2 cut(s)	247, 711
BseMI	GCAATG	1 cut(s)	1349
BseMII	CTCAG	3 cut(s)	500, 615, 1062
BseNI	ACTGG	4 cut(s)	920, 1015, 1402, 1416
BseRI	GAGGAG	3 cut(s)	46, 216, 724
BseXI	GCAGC	7 cut(s)	445, 767, 1147, 1150, 1213, 1222, 1225
BsgI	GTGCAG	2 cut(s)	1083, 1154
Bsh1236I	CGCG	1 cut(s)	1393
BshFI	GGCC	2 cut(s)	1059, 1280
BsiHKAI	GWGCWC	1 cut(s)	66
BsiHKCI	CYCGRG	1 cut(s)	77
BsiSI	CCGG	2 cut(s)	399, 693
BslFI	GGGAC	2 cut(s)	296, 331
BslI	CCNNNNNNNGG	2 cut(s)	247, 711
BsmAI	GTCTC	3 cut(s)	172, 400, 1323
BsmBI	CGTCTC	1 cut(s)	400
BsmFI	GGGAC	2 cut(s)	296, 331
BsmI	GAATGC	1 cut(s)	1237
BsnI	GGCC	2 cut(s)	1059, 1280
Bso31I	GGTCTC	1 cut(s)	1323
BsoBI	CYCGRG	1 cut(s)	77
Bsp119I	TTCGAA	1 cut(s)	1436
Bsp1286I	GDGCHC	2 cut(s)	66, 1371
Bsp13I	TCCGGA	1 cut(s)	398
Bsp1720I	GCTNAGC	1 cut(s)	1048
BspACI	CCGC	5 cut(s)	148, 171, 241, 732, 1201
BspANI	GGCC	2 cut(s)	1059, 1280
BspCNI	CTCAG	3 cut(s)	499, 614, 1063
BspEI	TCCGGA	1 cut(s)	398
BspFNI	CGCG	1 cut(s)	1393
BspHI	TCATGA	1 cut(s)	1162
BspLI	GGNNCC	2 cut(s)	403, 873
BspPI	GGATC	3 cut(s)	521, 620, 680
BspT104I	TTCGAA	1 cut(s)	1436
BspTNI	GGTCTC	1 cut(s)	1323
BsrBI	CCGCTC	3 cut(s)	243, 734, 1201
BsrDI	GCAATG	1 cut(s)	1349
BsrI	ACTGG	4 cut(s)	920, 1015, 1402, 1416
BssECI	CCNNGG	8 cut(s)	57, 76, 246, 348, 629, 989, 1281, 1447
BssSI	CACGAG	2 cut(s)	422, 974
BssT1I	CCWWGG	2 cut(s)	246, 1281
Bst2BI	CACGAG	2 cut(s)	422, 974
Bst2UI	CCWGG	5 cut(s)	350, 630, 990, 1104, 1334
Bst4CI	ACNGT	4 cut(s)	412, 784, 892, 1096
Bst6I	CTCTTC	3 cut(s)	217, 386, 1240
BstBI	TTCGAA	1 cut(s)	1436
BstDEI	CTNAG	4 cut(s)	486, 601, 1048, 1071
BstDSI	CCRYGG	1 cut(s)	1447
BstENI	CCTNNNNNAGG	1 cut(s)	709
BstF5I	GGATG	1 cut(s)	728
BstFNI	CGCG	1 cut(s)	1393
BstH2I	RGCGCY	1 cut(s)	207
BstHHI	GCGC	2 cut(s)	206, 1393
BstMAI	GTCTC	3 cut(s)	172, 400, 1323
BstMWI	GCNNNNNNNGC	7 cut(s)	147, 170, 203, 442, 1147, 1232, 1235
BstNI	CCWGG	5 cut(s)	350, 630, 990, 1104, 1334
BstSCI	CCNGG	5 cut(s)	348, 628, 988, 1102, 1332
BstSFI	CTRYAG	1 cut(s)	1466
BstUI	CGCG	1 cut(s)	1393
BstV1I	GCAGC	7 cut(s)	445, 767, 1147, 1150, 1213, 1222, 1225
BstV2I	GAAGAC	1 cut(s)	837
BstX2I	RGATCY	3 cut(s)	469, 625, 1174
BstYI	RGATCY	3 cut(s)	469, 625, 1174
Bsu36I	CCTNAGG	1 cut(s)	486
BsuI	GTATCC	1 cut(s)	326
BsuRI	GGCC	2 cut(s)	1059, 1280
BtgI	CCRYGG	1 cut(s)	1447
BtsCI	GGATG	1 cut(s)	728
BtsIMutI	CAGTG	2 cut(s)	913, 1409
CaiI	CAGNNNCTG	2 cut(s)	788, 1070
CciI	TCATGA	1 cut(s)	1162
CfoI	GCGC	2 cut(s)	206, 1393
Cfr13I	GGNCC	2 cut(s)	48, 1278
Csp6I	GTAC	2 cut(s)	1107, 1395
CspCI	CAANNNNNGTGG	2 cut(s)	564, 599
CviAII	CATG	4 cut(s)	143, 539, 970, 1163
CviQI	GTAC	2 cut(s)	1107, 1395
DdeI	CTNAG	4 cut(s)	486, 601, 1048, 1071
EaeI	YGGCCR	1 cut(s)	1057
Eam1104I	CTCTTC	3 cut(s)	217, 386, 1240
EarI	CTCTTC	3 cut(s)	217, 386, 1240
Eco130I	CCWWGG	2 cut(s)	246, 1281
Eco24I	GRGCYC	1 cut(s)	1371
Eco31I	GGTCTC	1 cut(s)	1323
Eco32I	GATATC	1 cut(s)	589
Eco47I	GGWCC	1 cut(s)	48
Eco57I	CTGAAG	1 cut(s)	6
Eco81I	CCTNAGG	1 cut(s)	486
Eco88I	CYCGRG	1 cut(s)	77
EcoNI	CCTNNNNNAGG	1 cut(s)	709
EcoRII	CCWGG	5 cut(s)	348, 628, 988, 1102, 1332
EcoRV	GATATC	1 cut(s)	589
EcoT14I	CCWWGG	2 cut(s)	246, 1281
EcoT38I	GRGCYC	1 cut(s)	1371
ErhI	CCWWGG	2 cut(s)	246, 1281
Esp3I	CGTCTC	1 cut(s)	400
FaeI	CATG	4 cut(s)	146, 542, 973, 1166
FalI	AAGNNNNNCTT	2 cut(s)	689, 721
FaqI	GGGAC	2 cut(s)	296, 331
FatI	CATG	4 cut(s)	142, 538, 969, 1162
FauI	CCCGC	2 cut(s)	248, 1194
FbaI	TGATCA	1 cut(s)	1300
Fnu4HI	GCNGC	7 cut(s)	434, 756, 1136, 1139, 1227, 1236, 1239
FokI	GGATG	1 cut(s)	715
FriOI	GRGCYC	1 cut(s)	1371
Fsp4HI	GCNGC	7 cut(s)	434, 756, 1136, 1139, 1227, 1236, 1239
FspBI	CTAG	4 cut(s)	174, 324, 531, 821
GlaI	GCGC	2 cut(s)	205, 1392
GluI	GCNGC	7 cut(s)	434, 756, 1136, 1139, 1227, 1236, 1239
HaeII	RGCGCY	1 cut(s)	207
HaeIII	GGCC	2 cut(s)	1059, 1280
HapII	CCGG	2 cut(s)	399, 693
HhaI	GCGC	2 cut(s)	206, 1393
Hin1II	CATG	4 cut(s)	146, 542, 973, 1166
Hin6I	GCGC	2 cut(s)	204, 1391
HinP1I	GCGC	2 cut(s)	204, 1391
HincII	GTYRAC	1 cut(s)	1291
HindII	GTYRAC	1 cut(s)	1291
HindIII	AAGCTT	2 cut(s)	178, 1148
HinfI	GANTC	4 cut(s)	344, 1115, 1421, 1433
HpaII	CCGG	2 cut(s)	399, 693
HphI	GGTGA	3 cut(s)	376, 628, 866
Hpy166II	GTNNAC	2 cut(s)	500, 1291
Hpy188III	TCNNGA	9 cut(s)	123, 399, 424, 767, 905, 926, 976, 1163, 1172
Hpy8I	GTNNAC	2 cut(s)	500, 1291
Hpy99I	CGWCG	1 cut(s)	395
HpyAV	CCTTC	6 cut(s)	61, 217, 240, 249, 454, 1020
HpyCH4III	ACNGT	4 cut(s)	412, 784, 892, 1096
HpyCH4IV	ACGT	1 cut(s)	1293
HpyCH4V	TGCA	7 cut(s)	649, 728, 1064, 1135, 1349, 1354, 1409
HpyF10VI	GCNNNNNNNGC	7 cut(s)	147, 170, 203, 442, 1147, 1232, 1235
HpyF3I	CTNAG	4 cut(s)	486, 601, 1048, 1071
HpySE526I	ACGT	1 cut(s)	1293
Hsp92II	CATG	4 cut(s)	146, 542, 973, 1166
HspAI	GCGC	2 cut(s)	204, 1391
Kpn2I	TCCGGA	1 cut(s)	398
Ksp22I	TGATCA	1 cut(s)	1300
LmnI	GCTCC	5 cut(s)	61, 248, 493, 739, 871
Lsp1109I	GCAGC	7 cut(s)	445, 767, 1147, 1150, 1213, 1222, 1225
LweI	GCATC	3 cut(s)	737, 784, 898
MaeI	CTAG	4 cut(s)	174, 324, 531, 821
MaeII	ACGT	1 cut(s)	1293
MaeIII	GTNAC	2 cut(s)	604, 616
MbiI	CCGCTC	3 cut(s)	243, 734, 1201
MboII	GAAGA	9 cut(s)	6, 204, 373, 479, 646, 837, 947, 1257, 1413
MfeI	CAATTG	1 cut(s)	477
MflI	RGATCY	3 cut(s)	469, 625, 1174
MhlI	GDGCHC	2 cut(s)	66, 1371
MluCI	AATT	8 cut(s)	477, 655, 681, 1183, 1205, 1221, 1377, 1404
MlyI	GAGTC	2 cut(s)	353, 1430
MmeI	TCCRAC	1 cut(s)	240
MroI	TCCGGA	1 cut(s)	398
MseI	TTAA	3 cut(s)	438, 659, 825
MslI	CAYNNNNRTG	4 cut(s)	141, 614, 918, 974
MspI	CCGG	2 cut(s)	399, 693
MspR9I	CCNGG	5 cut(s)	350, 630, 990, 1104, 1334
MunI	CAATTG	1 cut(s)	477
Mva1269I	GAATGC	1 cut(s)	1237
MvaI	CCWGG	5 cut(s)	350, 630, 990, 1104, 1334
MvnI	CGCG	1 cut(s)	1393
MwoI	GCNNNNNNNGC	7 cut(s)	147, 170, 203, 442, 1147, 1232, 1235
NlaIII	CATG	4 cut(s)	146, 542, 973, 1166
NlaIV	GGNNCC	2 cut(s)	403, 873
NmeAIII	GCCGAG	2 cut(s)	568, 1222
NmuCI	GTSAC	1 cut(s)	616
NspV	TTCGAA	1 cut(s)	1436
OliI	CACNNNNGTG	1 cut(s)	918
PagI	TCATGA	1 cut(s)	1162
PcsI	WCGNNNNNNNCGW	2 cut(s)	84, 282
PctI	GAATGC	1 cut(s)	1237
PfeI	GAWTC	2 cut(s)	1115, 1433
PkrI	GCNGC	7 cut(s)	435, 757, 1137, 1140, 1228, 1237, 1240
PleI	GAGTC	2 cut(s)	352, 1429
PpsI	GAGTC	2 cut(s)	352, 1429
PsiI	TTATAA	3 cut(s)	512, 1035, 1146
Psp6I	CCWGG	5 cut(s)	348, 628, 988, 1102, 1332
PspGI	CCWGG	5 cut(s)	348, 628, 988, 1102, 1332
PspN4I	GGNNCC	2 cut(s)	403, 873
PspPI	GGNCC	2 cut(s)	48, 1278
PstNI	CAGNNNCTG	2 cut(s)	788, 1070
PsuI	RGATCY	3 cut(s)	469, 625, 1174
RsaI	GTAC	2 cut(s)	1108, 1396
RsaNI	GTAC	2 cut(s)	1107, 1395
RseI	CAYNNNNRTG	4 cut(s)	141, 614, 918, 974
SaqAI	TTAA	3 cut(s)	438, 659, 825
SatI	GCNGC	7 cut(s)	434, 756, 1136, 1139, 1227, 1236, 1239
Sau96I	GGNCC	2 cut(s)	48, 1278
SchI	GAGTC	2 cut(s)	353, 1430
ScrFI	CCNGG	5 cut(s)	350, 630, 990, 1104, 1334
SduI	GDGCHC	2 cut(s)	66, 1371
SfaNI	GCATC	3 cut(s)	737, 784, 898
SfcI	CTRYAG	1 cut(s)	1466
SfuI	TTCGAA	1 cut(s)	1436
SinI	GGWCC	1 cut(s)	48
SmiMI	CAYNNNNRTG	4 cut(s)	141, 614, 918, 974
SmlI	CTYRAG	3 cut(s)	706, 773, 1316
SmoI	CTYRAG	3 cut(s)	706, 773, 1316
SpeI	ACTAGT	1 cut(s)	820
Sse9I	AATT	8 cut(s)	477, 655, 681, 1183, 1205, 1221, 1377, 1404
SsiI	CCGC	5 cut(s)	148, 171, 241, 732, 1201
SspMI	CTAG	4 cut(s)	174, 324, 531, 821
StyD4I	CCNGG	5 cut(s)	348, 628, 988, 1102, 1332
StyI	CCWWGG	2 cut(s)	246, 1281
TaaI	ACNGT	4 cut(s)	412, 784, 892, 1096
TaiI	ACGT	1 cut(s)	1296
TasI	AATT	8 cut(s)	477, 655, 681, 1183, 1205, 1221, 1377, 1404
TfiI	GAWTC	2 cut(s)	1115, 1433
Tru1I	TTAA	3 cut(s)	438, 659, 825
Tru9I	TTAA	3 cut(s)	438, 659, 825
TscAI	CASTG	2 cut(s)	920, 1416
TseFI	GTSAC	1 cut(s)	616
TseI	GCWGC	7 cut(s)	433, 755, 1135, 1138, 1226, 1235, 1238
Tsp45I	GTSAC	1 cut(s)	616
TspDTI	ATGAA	4 cut(s)	473, 750, 1128, 1151
TspGWI	ACGGA	2 cut(s)	831, 1436
TspRI	CASTG	2 cut(s)	920, 1416
VpaK11BI	GGWCC	1 cut(s)	48
XagI	CCTNNNNNAGG	1 cut(s)	709
XapI	RAATTY	2 cut(s)	655, 1377
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1126
XspI	CTAG	4 cut(s)	174, 324, 531, 821

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.