AT2G32120
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009614
Physical Location & Seq
Reverse (-)
13651442 .. 13653945
2504 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32120.2

Sequence Viewer

Length: 1692 bp
ATGGCAGAAGCAGCATACACAGTAGCATCAGACAGTGAAAACACTGGAGAGGAGAAATCATCATCATCTCCTTCATTACCCGAAATCGCCCTTGGTATCGACATTGGTACTTCTCAATGCAGTATAGCTGTTTGGAACGGTTCTCAAGTTCACATCTTGAGGAACACAAGAAACCAGAAGCTCATCAAATCATTTGTCACTTTCAAAGATGAAGTTCCTGCTGGTGGTGTTAGCAACCAGCTCGCACATGAGCAGGAAATGCTAACCGGAGCCGCTATCTTCAACATGAAGCGGCTCGTTGGTCGTGTAGACACTGATCCTGTGGTTCACGCTAGCAAGAACCTTCCTTTCTTGGTTCAAACTCTTGATATTGGAGTTAGACCGTTTATTGCAGCTTTGGTGAACAATGCTTGGAGATCAACAACACCAGAGGAAGTTTTAGCTATATTTCTGGTGGAGTTACGTCTGATGGCTGAAGCTCAGTTGAAACGTCCTGTGAGAAATGTAGTGCTTACGGTTCCGGTTTCGTTCTCTAGGTTCCAGCTCACACGGTTCGAAAGAGCTTGCGCTATGGCTGGACTTCATGTTCTTCGTTTGATGCCGGAACCAACTGCTATTGCGTTGCTTTATGCGCAACAGCAGCAGATGACTACCCATGATAACATGGGAAGCGGAAGCGAGAGGCTTGCGGTTATATTCAATATGGGAGCTGGTTACTGCGATGTTGCGGTTACTGCTACTGCTGGTGGTGTTTCACAGATAAAAGCTTTAGCTGGTAGCCCCATTGGGGGTGAAGACATTTTGCAGAACACAATTCGCCATATCGCTCCACCTAATGAAGAAGCTTCGGGGTTGCTTCGTGTAGCGGCACAGGACGCGATTCACAGGCTAACGGATCAAGAAAATGTCCAAATTGAAGTGGATTTGGGAAATGGTAACAAGATATCCAAGGTTCTTGATAGGTTAGAGTTTGAGGAAGTGAACCAGAAGGTATTTGAGGAATGTGAGAGACTTGTTGTGCAGTGCCTGCGAGATGCGAGAGTCAATGGTGGTGATATCGATGATTTGATAATGGTTGGAGGGTGTTCGTACATCCCGAAAGTAAGAACTATTATCAAGAACGTATGCAAGAAGGATGAGATATACAAAGGCGTGAATCCTTTAGAAGCTGCGGTTAGAGGAGCTGCTTTGGAAGGTGCGGTGACTTCAGGGATTCATGATCCTTTTGGGAGCTTAGATCTGTTAACCATACAAGCCACACCTCTTGCAGTTGGAGTAAGAGCTAACGGAAACAAATTCATACCCGTGATTCCGCGTAACACAATGGTTCCAGCGCGGAAAGACCTCTTCTTCACAACGGTTCAAGACAACCAGAAGGAAGCTCTGATCATTATATACGAAGGAGAAGGAGAGACTGTTGAAGAGAATCATCTTCTTGGTTATTTCAAGCTCGTTGGGATTCCGCCAGCACCGAAAGGTGTTCCAGAGATCAATGTGTGTATGGACATTGATGCATCAAATGCTTTACGGGTTTTCGCAGCTGTGTTGATGCCGGGATCTTCGAGTCCAGTGGTTCCTGTGATTGAGGTGAGGATGCCTACGGTTGATGATGGACATGGTTGGTGTGCTCAAGCTTTGAATGTGAAATATGGAGCTACTCTTGATTTGATTACTCTTCAGAGAAAGATGTAA

Protein Analysis

563

Amino Acids

60.99

Weight (kDa)

5.46

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 31 - 517 1.4e-89 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 633
AccI GTMKAC 1 cut(s) 309
AccII CGCG 3 cut(s) 878, 1315, 1336
AclWI GGATC 4 cut(s) 311, 903, 1214, 1564
AcsI RAATTY 1 cut(s) 1295
AcuI CTGAAG 3 cut(s) 495, 1191, 1661
AfaI GTAC 2 cut(s) 109, 1091
AfiI CCNNNNNNNGG 3 cut(s) 224, 787, 788
AjuI GAANNNNNNNTTGG 2 cut(s) 75, 107
AloI GAACNNNNNNTCC 2 cut(s) 570, 602
Alw21I GWGCWC 1 cut(s) 1630
Alw26I GTCTC 2 cut(s) 1003, 1406
AlwI GGATC 4 cut(s) 311, 903, 1214, 1564
AlwNI CAGNNNCTG 1 cut(s) 1027
ApeKI GCWGC 6 cut(s) 11, 392, 640, 1169, 1184, 1538
ApoI RAATTY 1 cut(s) 1295
ArsI GACNNNNNNTTYG 2 cut(s) 180, 212
AspLEI GCGC 3 cut(s) 569, 634, 1336
AsuC2I CCSGG 1 cut(s) 1554
AsuHPI GGTGA 5 cut(s) 412, 803, 1064, 1213, 1600
AsuII TTCGAA 1 cut(s) 555
AsuNHI GCTAGC 1 cut(s) 332
BbsI GAAGAC 1 cut(s) 801
Bbv12I GWGCWC 1 cut(s) 1630
BbvI GCAGC 6 cut(s) 23, 404, 652, 1156, 1171, 1550
BccI CCATC 2 cut(s) 463, 1604
BcgI CGANNNNNNTGC 4 cut(s) 223, 257, 668, 702
BclI TGATCA 1 cut(s) 1386
BcnI CCSGG 1 cut(s) 1554
BcoDI GTCTC 2 cut(s) 1003, 1406
BfaI CTAG 2 cut(s) 333, 534
BglII AGATCT 1 cut(s) 1237
BisI GCNGC 9 cut(s) 12, 273, 293, 393, 641, 867, 1170, 1185, 1539
BlsI GCNGC 9 cut(s) 13, 274, 294, 394, 642, 868, 1171, 1186, 1540
Bme1390I CCNGG 1 cut(s) 1554
BmiI GGNNCC 6 cut(s) 271, 519, 539, 606, 1329, 1575
BmrFI CCNGG 1 cut(s) 1554
BmsI GCATC 7 cut(s) 35, 588, 1024, 1501, 1523, 1539, 1584
BmtI GCTAGC 1 cut(s) 336
BpiI GAAGAC 1 cut(s) 801
BpmI CTGGAG 1 cut(s) 66
Bpu14I TTCGAA 1 cut(s) 555
BpuEI CTTGAG 3 cut(s) 129, 178, 1614
BpuMI CCSGG 1 cut(s) 1554
Bsa29I ATCGAT 1 cut(s) 1059
BsaJI CCNNGG 2 cut(s) 91, 948
BsaWI WCCGGW 2 cut(s) 266, 520
BsaXI ACNNNNNCTCC 2 cut(s) 1401, 1431
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 787, 788
Bse1I ACTGG 2 cut(s) 49, 1568
BseCI ATCGAT 1 cut(s) 1059
BseDI CCNNGG 2 cut(s) 91, 948
BseGI GGATG 3 cut(s) 1092, 1141, 1599
BseLI CCNNNNNNNGG 3 cut(s) 224, 787, 788
BseMII CTCAG 1 cut(s) 494
BseNI ACTGG 2 cut(s) 49, 1568
BseRI GAGGAG 2 cut(s) 65, 1194
BseXI GCAGC 6 cut(s) 23, 404, 652, 1156, 1171, 1550
BsgI GTGCAG 1 cut(s) 1040
Bsh1236I CGCG 3 cut(s) 878, 1315, 1336
BshVI ATCGAT 1 cut(s) 1059
BsiHKAI GWGCWC 1 cut(s) 1630
BsiSI CCGG 4 cut(s) 267, 521, 602, 1553
BslI CCNNNNNNNGG 3 cut(s) 224, 787, 788
BsmAI GTCTC 2 cut(s) 1003, 1406
Bsp119I TTCGAA 1 cut(s) 555
Bsp1286I GDGCHC 1 cut(s) 1630
Bsp143I GATC 8 cut(s) 316, 416, 895, 1219, 1237, 1386, 1488, 1556
BspCNI CTCAG 1 cut(s) 493
BspDI ATCGAT 1 cut(s) 1059
BspFNI CGCG 3 cut(s) 878, 1315, 1336
BspHI TCATGA 1 cut(s) 1216
BspLI GGNNCC 6 cut(s) 271, 519, 539, 606, 1329, 1575
BspOI GCTAGC 1 cut(s) 336
BspPI GGATC 4 cut(s) 311, 903, 1214, 1564
BspT104I TTCGAA 1 cut(s) 555
BsrI ACTGG 2 cut(s) 49, 1568
BssECI CCNNGG 2 cut(s) 91, 948
BssMI GATC 8 cut(s) 316, 416, 895, 1219, 1237, 1386, 1488, 1556
BssT1I CCWWGG 2 cut(s) 91, 948
Bst4CI ACNGT 9 cut(s) 22, 35, 140, 384, 517, 552, 1360, 1417, 1603
Bst6I CTCTTC 3 cut(s) 1352, 1416, 1680
BstAPI GCANNNNNTGC 3 cut(s) 259, 1027, 1520
BstBI TTCGAA 1 cut(s) 555
BstC8I GCNNGC 6 cut(s) 243, 334, 565, 687, 1028, 1467
BstDEI CTNAG 2 cut(s) 480, 1234
BstF5I GGATG 3 cut(s) 1092, 1141, 1599
BstFNI CGCG 3 cut(s) 878, 1315, 1336
BstHHI GCGC 3 cut(s) 569, 634, 1336
BstKTI GATC 8 cut(s) 319, 419, 898, 1222, 1240, 1389, 1491, 1559
BstMAI GTCTC 2 cut(s) 1003, 1406
BstMBI GATC 8 cut(s) 316, 416, 895, 1219, 1237, 1386, 1488, 1556
BstMWI GCNNNNNNNGC 8 cut(s) 11, 259, 631, 640, 734, 875, 1027, 1520
BstSCI CCNGG 1 cut(s) 1552
BstUI CGCG 3 cut(s) 878, 1315, 1336
BstV1I GCAGC 6 cut(s) 23, 404, 652, 1156, 1171, 1550
BstV2I GAAGAC 1 cut(s) 801
BstX2I RGATCY 2 cut(s) 1237, 1556
BstYI RGATCY 2 cut(s) 1237, 1556
Bsu15I ATCGAT 1 cut(s) 1059
BsuTUI ATCGAT 1 cut(s) 1059
BtgZI GCGATG 1 cut(s) 735
BtsCI GGATG 3 cut(s) 1092, 1141, 1599
BtsI GCAGTG 1 cut(s) 1028
BtsIMutI CAGTG 5 cut(s) 40, 42, 312, 1028, 1575
Cac8I GCNNGC 6 cut(s) 243, 334, 565, 687, 1028, 1467
CaiI CAGNNNCTG 1 cut(s) 1027
CciI TCATGA 1 cut(s) 1216
CfoI GCGC 3 cut(s) 569, 634, 1336
ClaI ATCGAT 1 cut(s) 1059
CseI GACGC 1 cut(s) 884
Csp6I GTAC 2 cut(s) 108, 1090
CspCI CAANNNNNGTGG 2 cut(s) 1246, 1281
CviAII CATG 7 cut(s) 248, 286, 584, 656, 664, 1217, 1616
CviQI GTAC 2 cut(s) 108, 1090
DdeI CTNAG 2 cut(s) 480, 1234
DpnI GATC 8 cut(s) 318, 418, 897, 1221, 1239, 1388, 1490, 1558
DpnII GATC 8 cut(s) 316, 416, 895, 1219, 1237, 1386, 1488, 1556
Eam1104I CTCTTC 3 cut(s) 1352, 1416, 1680
EarI CTCTTC 3 cut(s) 1352, 1416, 1680
EciI GGCGGA 1 cut(s) 1452
Eco130I CCWWGG 2 cut(s) 91, 948
Eco32I GATATC 2 cut(s) 945, 1057
Eco57I CTGAAG 3 cut(s) 495, 1191, 1661
EcoRV GATATC 2 cut(s) 945, 1057
EcoT14I CCWWGG 2 cut(s) 91, 948
EcoT22I ATGCAT 1 cut(s) 1516
ErhI CCWWGG 2 cut(s) 91, 948
FaeI CATG 7 cut(s) 251, 289, 587, 659, 667, 1220, 1619
FatI CATG 7 cut(s) 247, 285, 583, 655, 663, 1216, 1615
FbaI TGATCA 1 cut(s) 1386
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 9 cut(s) 12, 273, 293, 393, 641, 867, 1170, 1185, 1539
FokI GGATG 3 cut(s) 1079, 1148, 1606
Fsp4HI GCNGC 9 cut(s) 12, 273, 293, 393, 641, 867, 1170, 1185, 1539
FspBI CTAG 2 cut(s) 333, 534
FspI TGCGCA 1 cut(s) 633
GlaI GCGC 3 cut(s) 568, 633, 1335
GluI GCNGC 9 cut(s) 12, 273, 293, 393, 641, 867, 1170, 1185, 1539
GsuI CTGGAG 1 cut(s) 66
HapII CCGG 4 cut(s) 267, 521, 602, 1553
HgaI GACGC 1 cut(s) 884
HhaI GCGC 3 cut(s) 569, 634, 1336
Hin1II CATG 7 cut(s) 251, 289, 587, 659, 667, 1220, 1619
Hin6I GCGC 3 cut(s) 567, 632, 1334
HinP1I GCGC 3 cut(s) 567, 632, 1334
HincII GTYRAC 1 cut(s) 1245
HindII GTYRAC 1 cut(s) 1245
HindIII AAGCTT 3 cut(s) 765, 843, 1632
HinfI GANTC 8 cut(s) 880, 1041, 1156, 1213, 1309, 1426, 1459, 1564
HpaI GTTAAC 1 cut(s) 1245
HpaII CCGG 4 cut(s) 267, 521, 602, 1553
HphI GGTGA 5 cut(s) 412, 803, 1064, 1213, 1600
Hpy166II GTNNAC 6 cut(s) 151, 310, 328, 403, 982, 1245
Hpy188I TCNGA 4 cut(s) 31, 468, 1386, 1680
Hpy8I GTNNAC 6 cut(s) 151, 310, 328, 403, 982, 1245
HpyAV CCTTC 8 cut(s) 81, 353, 982, 1126, 1187, 1369, 1394, 1400
HpyCH4III ACNGT 9 cut(s) 22, 35, 140, 384, 517, 552, 1360, 1417, 1603
HpyCH4IV ACGT 3 cut(s) 463, 490, 1122
HpyCH4V TGCA 7 cut(s) 120, 392, 805, 1021, 1128, 1268, 1514
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 259, 631, 640, 734, 875, 1027, 1520
HpyF3I CTNAG 2 cut(s) 480, 1234
HpySE526I ACGT 3 cut(s) 463, 490, 1122
Hsp92II CATG 7 cut(s) 251, 289, 587, 659, 667, 1220, 1619
HspAI GCGC 3 cut(s) 567, 632, 1334
Ksp22I TGATCA 1 cut(s) 1386
KspAI GTTAAC 1 cut(s) 1245
Kzo9I GATC 8 cut(s) 316, 416, 895, 1219, 1237, 1386, 1488, 1556
LmnI GCTCC 6 cut(s) 269, 707, 832, 1181, 1230, 1652
Lsp1109I GCAGC 6 cut(s) 23, 404, 652, 1156, 1171, 1550
LweI GCATC 7 cut(s) 35, 588, 1024, 1501, 1523, 1539, 1584
MaeI CTAG 2 cut(s) 333, 534
MaeII ACGT 3 cut(s) 463, 490, 1122
MaeIII GTNAC 7 cut(s) 196, 459, 713, 730, 935, 1201, 1316
MalI GATC 8 cut(s) 318, 418, 897, 1221, 1239, 1388, 1490, 1558
MboI GATC 8 cut(s) 316, 416, 895, 1219, 1237, 1386, 1488, 1556
MflI RGATCY 2 cut(s) 1237, 1556
MhlI GDGCHC 1 cut(s) 1630
MluCI AATT 3 cut(s) 813, 912, 1295
MlyI GAGTC 2 cut(s) 1050, 1573
MmeI TCCRAC 2 cut(s) 1057, 1252
Mph1103I ATGCAT 1 cut(s) 1516
MseI TTAA 1 cut(s) 1244
MslI CAYNNNNRTG 1 cut(s) 1304
MspA1I CMGCKG 1 cut(s) 1541
MspI CCGG 4 cut(s) 267, 521, 602, 1553
MspR9I CCNGG 1 cut(s) 1554
MvnI CGCG 3 cut(s) 878, 1315, 1336
MwoI GCNNNNNNNGC 8 cut(s) 11, 259, 631, 640, 734, 875, 1027, 1520
NciI CCSGG 1 cut(s) 1554
NdeII GATC 8 cut(s) 316, 416, 895, 1219, 1237, 1386, 1488, 1556
NheI GCTAGC 1 cut(s) 332
NlaIII CATG 7 cut(s) 251, 289, 587, 659, 667, 1220, 1619
NlaIV GGNNCC 6 cut(s) 271, 519, 539, 606, 1329, 1575
NmuCI GTSAC 2 cut(s) 196, 1201
NsbI TGCGCA 1 cut(s) 633
NsiI ATGCAT 1 cut(s) 1516
NspV TTCGAA 1 cut(s) 555
PagI TCATGA 1 cut(s) 1216
PcsI WCGNNNNNNNCGW 1 cut(s) 1094
PfeI GAWTC 6 cut(s) 880, 1156, 1213, 1309, 1426, 1459
PkrI GCNGC 9 cut(s) 13, 274, 294, 394, 642, 868, 1171, 1186, 1540
PleI GAGTC 2 cut(s) 1049, 1572
PpsI GAGTC 2 cut(s) 1049, 1572
PspN4I GGNNCC 6 cut(s) 271, 519, 539, 606, 1329, 1575
PstNI CAGNNNCTG 1 cut(s) 1027
PsuI RGATCY 2 cut(s) 1237, 1556
PvuII CAGCTG 1 cut(s) 1541
RsaI GTAC 2 cut(s) 109, 1091
RsaNI GTAC 2 cut(s) 108, 1090
RseI CAYNNNNRTG 1 cut(s) 1304
SaqAI TTAA 1 cut(s) 1244
SatI GCNGC 9 cut(s) 12, 273, 293, 393, 641, 867, 1170, 1185, 1539
Sau3AI GATC 8 cut(s) 316, 416, 895, 1219, 1237, 1386, 1488, 1556
SchI GAGTC 2 cut(s) 1050, 1573
ScrFI CCNGG 1 cut(s) 1554
SduI GDGCHC 1 cut(s) 1630
SfaNI GCATC 7 cut(s) 35, 588, 1024, 1501, 1523, 1539, 1584
SfuI TTCGAA 1 cut(s) 555
SmiMI CAYNNNNRTG 1 cut(s) 1304
SmlI CTYRAG 3 cut(s) 144, 157, 1629
SmoI CTYRAG 3 cut(s) 144, 157, 1629
Sse9I AATT 3 cut(s) 813, 912, 1295
SspMI CTAG 2 cut(s) 333, 534
StyD4I CCNGG 1 cut(s) 1552
StyI CCWWGG 2 cut(s) 91, 948
TaaI ACNGT 9 cut(s) 22, 35, 140, 384, 517, 552, 1360, 1417, 1603
TaiI ACGT 3 cut(s) 466, 493, 1125
TaqI TCGA 4 cut(s) 99, 555, 1059, 1562
TasI AATT 3 cut(s) 813, 912, 1295
TauI GCSGC 3 cut(s) 275, 295, 869
TfiI GAWTC 6 cut(s) 880, 1156, 1213, 1309, 1426, 1459
Tru1I TTAA 1 cut(s) 1244
Tru9I TTAA 1 cut(s) 1244
TscAI CASTG 5 cut(s) 40, 49, 319, 1028, 1575
TseFI GTSAC 2 cut(s) 196, 1201
TseI GCWGC 6 cut(s) 11, 392, 640, 1169, 1184, 1538
Tsp45I GTSAC 2 cut(s) 196, 1201
TspDTI ATGAA 7 cut(s) 63, 225, 302, 572, 852, 1205, 1288
TspGWI ACGGA 2 cut(s) 908, 1302
TspRI CASTG 5 cut(s) 40, 49, 319, 1028, 1575
XapI RAATTY 1 cut(s) 1295
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 2 cut(s) 333, 534
Zsp2I ATGCAT 1 cut(s) 1516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.