MD16G1192600.v1.1
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009614
Physical Location & Seq
Forward (+)
17025352 .. 17028024
2673 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1192600.v1.1.491

Sequence Viewer

Length: 1749 bp
ATGTCTGCATGCTACGAAAGCCGAAGGGTATCAGCAGGAATGGCTGAGCCAGCATACACAGTCGCATCTGATAGTGAAACCGCTGGGGAGGAAAAATCGTCGTCTCCTTTTCCTGAAACCGCTATTGGGATTGACATCGGCACTTCACATTGCAGTGTTGCTGTCTGGAATGGCTCCCAGGTTGAGCTCTTGAGGAACATTAGAAACCAGAAAATGATGCGGTCGTATGTCACATTCAAGGATGAAAACCCTTCAGCGGGAGTAAGCGATCAACTCTCCAGTGAGCACGACATGTTATCTGGAGCTTCAATCTTCAACATGAAACGATTAATTGGCAGGGTTGACACCGATCCAGTTGTTCAGTCAAGCAAAAGCCTCCCCTTTTTGGTTCAAACATTGGGCATTGGGGTGCGCCCACTGATCGCAGCCTTGGTGAACAATGTATGGAGATCCACCACTCCTGAAGAAGTTCTGGCAATATTTTTGGTGGAACTCAAGGCAATGGCAGAAATCCAGCTAAAGCGGCCCATAAAGAATGTTGTTCTCACCATTCCAGCTTCATTCAGCCGATTCCAGCTGACTCGTATTGAAAGAGCTTGTGCCATGGCTAGCCTTCATGTACTCAGACTTATGCCTGAACCAACTGCTGTCGCGCTGTTATACGCGCAACAGATTTCAAATCAGAACGTAAACACCGGATCTGAGAAGATTGCTCTAATCTTCAACATGGGTGCTGGATACTGTGATGTAGCTATTACTGCTACAGCTGGAGGAGTTTCCCAAATAAAAGCCTTAGCAGGATCTGCCATTGGAGGGGAAGACTTGCTTCTGAGTATGATGCGTCATCTGTTGCCAGATTCCGAGACTCTTTTAACTAGCCACGGACTTGATGACATAAAGTCAGTTGGCATACTTCGAGTTGCAACACAGGAGGCAATCCACACTCTCTCCTCCCAAACTAGCGTGCAGATTGATGTTGACTTGGGAAATGGGACAAAAATATGTAAGGTTGTGGATCGGGAGGAATTTGAGGAAGTTAACCTAGCAGTGTTTGATAAGTGTGCGAGCCTCATAAGCCAGTGCTTGCATGATGCAAAGGTTGATACCGAGGATGTGAGCGATGTAATAGTTGTTGGCGGATGCTCATATATTCCAAAGATAAAAAGTCTTGTCATGAGCATTTGTAAAAAGGAGGAGCTGTACAAAGGGATGAAACCGTTGGAAGCTGCTGTCATTGGAGCGGCACTAGAAGGAGCGGTGGCATCAGGTCTTAGCGATCCCTTTGGGAGTTTGGACTTGCTTACCATTCAAGCCACCCCTCTTGCCCTTGGGATCCGAGCTGATGGAAACAACTTTGTGCCTATCATACCTAGAAACACAGCAATGCCAGCACAGAAAGATCTGATCTTCACAACTGCCCATGATAATCAAACTGAAGCACTAATAATCGTCTACGAAGGCGAGGGGAAGAAGGTGGAAGAGAACCATTTGCTGGGGTATTTCAAGATCACTGGAATTCCACCGTCCCCGAAAGGAGTTCCGGAAATAAGAGTAATTCTGGACATCGATGCATCAAGCGTGCTGAGAGTTTTGGCCGGGGTTTTAATGCCGGGGTCTCATCAGCCAATAAATCCAGTCATGGGAGTAAGGATGCCAACAGTTGATGATGGTCACGGTTGGTGCGCTGAAGCCCTACACAGAGCTTATGGCTCGTCTCTAGACTTGGTTACAGTGCAGAAGAAGATATAG

Protein Analysis

583

Amino Acids

62.46

Weight (kDa)

5.42

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 41 - 541 1.7e-96 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1492
AccBSI CCGCTC 2 cut(s) 1241, 1256
AccI GTMKAC 1 cut(s) 1452
AccII CGCG 2 cut(s) 653, 665
AccIII TCCGGA 1 cut(s) 1540
AciI CCGC 8 cut(s) 81, 120, 220, 257, 523, 1137, 1241, 1256
AclWI GGATC 8 cut(s) 344, 444, 706, 808, 1023, 1271, 1327, 1340
AcoI YGGCCR 1 cut(s) 1593
AcsI RAATTY 2 cut(s) 1025, 1515
AcuI CTGAAG 4 cut(s) 237, 483, 1455, 1707
AfaI GTAC 2 cut(s) 621, 1202
AfiI CCNNNNNNNGG 7 cut(s) 126, 256, 257, 385, 813, 1492, 1640
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 9 cut(s) 238, 309, 316, 392, 590, 678, 724, 1310, 1504
AhdI GACNNNNNGTC 1 cut(s) 898
AjnI CCWGG 1 cut(s) 177
AjuI GAANNNNNNNTTGG 2 cut(s) 108, 140
Alw21I GWGCWC 2 cut(s) 189, 288
Alw26I GTCTC 4 cut(s) 108, 857, 1620, 1719
AlwI GGATC 8 cut(s) 344, 444, 706, 808, 1023, 1271, 1327, 1340
AlwNI CAGNNNCTG 1 cut(s) 803
Aor13HI TCCGGA 1 cut(s) 1540
AoxI GGCC 2 cut(s) 524, 1593
ApeKI GCWGC 2 cut(s) 425, 1226
ApoI RAATTY 2 cut(s) 1025, 1515
AseI ATTAAT 1 cut(s) 329
Asp700I GAANNNNTTC 1 cut(s) 468
AspLEI GCGC 4 cut(s) 414, 655, 667, 1685
AspS9I GGNCC 1 cut(s) 525
AsuC2I CCSGG 2 cut(s) 1597, 1611
AsuHPI GGTGA 2 cut(s) 445, 538
AsuNHI GCTAGC 1 cut(s) 608
BamHI GGATCC 1 cut(s) 1332
BanII GRGCYC 1 cut(s) 189
BbsI GAAGAC 1 cut(s) 825
Bbv12I GWGCWC 2 cut(s) 189, 288
BbvI GCAGC 2 cut(s) 437, 1213
BccI CCATC 2 cut(s) 1337, 1661
BciT130I CCWGG 1 cut(s) 179
BciVI GTATCC 1 cut(s) 731
BcnI CCSGG 2 cut(s) 1597, 1611
BcoDI GTCTC 4 cut(s) 108, 857, 1620, 1719
BfaI CTAG 7 cut(s) 609, 876, 960, 1043, 1247, 1371, 1718
BfmI CTRYAG 1 cut(s) 762
BfuI GTATCC 1 cut(s) 731
BglII AGATCT 1 cut(s) 1399
BisI GCNGC 4 cut(s) 426, 524, 1227, 1242
BlpI GCTNAGC 1 cut(s) 45
BlsI GCNGC 4 cut(s) 427, 525, 1228, 1243
Bme1390I CCNGG 3 cut(s) 179, 1597, 1611
BmeRI GACNNNNNGTC 1 cut(s) 898
BmgT120I GGNCC 1 cut(s) 525
BmiI GGNNCC 2 cut(s) 175, 1334
BmrFI CCNGG 3 cut(s) 179, 1597, 1611
BmsI GCATC 9 cut(s) 74, 207, 828, 1081, 1130, 1271, 1558, 1580, 1641
BmtI GCTAGC 1 cut(s) 612
BpiI GAAGAC 1 cut(s) 825
BpmI CTGGAG 3 cut(s) 262, 321, 789
Bpu10I CCTNAGC 1 cut(s) 793
Bpu1102I GCTNAGC 1 cut(s) 45
BpuEI CTTGAG 2 cut(s) 211, 479
BpuMI CCSGG 2 cut(s) 1597, 1611
Bsa29I ATCGAT 1 cut(s) 1566
BsaBI GATNNNNATC 1 cut(s) 134
BsaI GGTCTC 1 cut(s) 1620
BsaJI CCNNGG 8 cut(s) 177, 429, 603, 880, 1107, 1327, 1596, 1610
BsaWI WCCGGW 2 cut(s) 695, 1540
BsaXI ACNNNNNCTCC 4 cut(s) 932, 962, 1184, 1214
Bsc4I CCNNNNNNNGG 7 cut(s) 126, 256, 257, 385, 813, 1492, 1640
Bse1I ACTGG 5 cut(s) 279, 353, 1078, 1516, 1634
Bse3DI GCAATG 3 cut(s) 148, 507, 1389
Bse8I GATNNNNATC 1 cut(s) 134
BseAI TCCGGA 1 cut(s) 1540
BseBI CCWGG 1 cut(s) 179
BseCI ATCGAT 1 cut(s) 1566
BseDI CCNNGG 8 cut(s) 177, 429, 603, 880, 1107, 1327, 1596, 1610
BseGI GGATG 5 cut(s) 247, 1117, 1145, 1215, 1656
BseJI GATNNNNATC 1 cut(s) 134
BseLI CCNNNNNNNGG 7 cut(s) 126, 256, 257, 385, 813, 1492, 1640
BseMI GCAATG 3 cut(s) 148, 507, 1389
BseMII CTCAG 5 cut(s) 36, 637, 693, 821, 1574
BseNI ACTGG 5 cut(s) 279, 353, 1078, 1516, 1634
BseRI GAGGAG 3 cut(s) 786, 940, 1208
BseXI GCAGC 2 cut(s) 437, 1213
BseYI CCCAGC 2 cut(s) 83, 1492
BsgI GTGCAG 1 cut(s) 986
Bsh1236I CGCG 2 cut(s) 653, 665
Bsh1285I CGRYCG 1 cut(s) 224
BshFI GGCC 2 cut(s) 526, 1595
BshVI ATCGAT 1 cut(s) 1566
BsiEI CGRYCG 1 cut(s) 224
BsiHKAI GWGCWC 2 cut(s) 189, 288
BsiSI CCGG 4 cut(s) 696, 1541, 1596, 1610
BslFI GGGAC 2 cut(s) 1006, 1510
BslI CCNNNNNNNGG 7 cut(s) 126, 256, 257, 385, 813, 1492, 1640
BsmAI GTCTC 4 cut(s) 108, 857, 1620, 1719
BsmBI CGTCTC 2 cut(s) 108, 1719
BsmFI GGGAC 2 cut(s) 1006, 1510
BsnI GGCC 2 cut(s) 526, 1595
Bso31I GGTCTC 1 cut(s) 1620
Bsp1286I GDGCHC 2 cut(s) 189, 288
Bsp13I TCCGGA 1 cut(s) 1540
Bsp1407I TGTACA 1 cut(s) 1200
Bsp1720I GCTNAGC 1 cut(s) 45
Bsp19I CCATGG 1 cut(s) 603
BspACI CCGC 8 cut(s) 81, 120, 220, 257, 523, 1137, 1241, 1256
BspANI GGCC 2 cut(s) 526, 1595
BspCNI CTCAG 5 cut(s) 37, 636, 694, 822, 1575
BspDI ATCGAT 1 cut(s) 1566
BspEI TCCGGA 1 cut(s) 1540
BspFNI CGCG 2 cut(s) 653, 665
BspHI TCATGA 1 cut(s) 1173
BspLI GGNNCC 2 cut(s) 175, 1334
BspOI GCTAGC 1 cut(s) 612
BspPI GGATC 8 cut(s) 344, 444, 706, 808, 1023, 1271, 1327, 1340
BspTNI GGTCTC 1 cut(s) 1620
BsrBI CCGCTC 2 cut(s) 1241, 1256
BsrDI GCAATG 3 cut(s) 148, 507, 1389
BsrGI TGTACA 1 cut(s) 1200
BsrI ACTGG 5 cut(s) 279, 353, 1078, 1516, 1634
BssECI CCNNGG 8 cut(s) 177, 429, 603, 880, 1107, 1327, 1596, 1610
BssT1I CCWWGG 3 cut(s) 429, 603, 1327
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 7 cut(s) 61, 743, 1218, 1524, 1660, 1676, 1732
Bst6I CTCTTC 1 cut(s) 1473
BstAPI GCANNNNNTGC 1 cut(s) 803
BstAUI TGTACA 1 cut(s) 1200
BstC8I GCNNGC 8 cut(s) 10, 51, 610, 965, 1066, 1085, 1389, 1580
BstDEI CTNAG 7 cut(s) 45, 623, 702, 793, 830, 1271, 1583
BstDSI CCRYGG 2 cut(s) 603, 880
BstF5I GGATG 5 cut(s) 247, 1117, 1145, 1215, 1656
BstFNI CGCG 2 cut(s) 653, 665
BstHHI GCGC 4 cut(s) 414, 655, 667, 1685
BstMAI GTCTC 4 cut(s) 108, 857, 1620, 1719
BstMCI CGRYCG 1 cut(s) 224
BstMWI GCNNNNNNNGC 8 cut(s) 18, 41, 50, 523, 758, 803, 1074, 1388
BstNI CCWGG 1 cut(s) 179
BstNSI RCATGY 2 cut(s) 12, 295
BstSCI CCNGG 3 cut(s) 177, 1595, 1609
BstSFI CTRYAG 1 cut(s) 762
BstUI CGCG 2 cut(s) 653, 665
BstV1I GCAGC 2 cut(s) 437, 1213
BstV2I GAAGAC 1 cut(s) 825
BstX2I RGATCY 5 cut(s) 449, 698, 800, 1332, 1399
BstYI RGATCY 5 cut(s) 449, 698, 800, 1332, 1399
Bsu15I ATCGAT 1 cut(s) 1566
BsuI GTATCC 1 cut(s) 731
BsuRI GGCC 2 cut(s) 526, 1595
BsuTUI ATCGAT 1 cut(s) 1566
BtgI CCRYGG 2 cut(s) 603, 880
BtgZI GCGATG 1 cut(s) 1134
BtsCI GGATG 5 cut(s) 247, 1117, 1145, 1215, 1656
BtsI GCAGTG 2 cut(s) 160, 1053
BtsIMutI CAGTG 7 cut(s) 160, 286, 416, 1053, 1085, 1509, 1737
Cac8I GCNNGC 8 cut(s) 10, 51, 610, 965, 1066, 1085, 1389, 1580
CaiI CAGNNNCTG 1 cut(s) 803
CciI TCATGA 1 cut(s) 1173
CfoI GCGC 4 cut(s) 414, 655, 667, 1685
Cfr13I GGNCC 1 cut(s) 525
ClaI ATCGAT 1 cut(s) 1566
CseI GACGC 1 cut(s) 830
Csp6I GTAC 2 cut(s) 620, 1201
CspCI CAANNNNNGTGG 2 cut(s) 1303, 1338
CviQI GTAC 2 cut(s) 620, 1201
DdeI CTNAG 7 cut(s) 45, 623, 702, 793, 830, 1271, 1583
DriI GACNNNNNGTC 1 cut(s) 898
EaeI YGGCCR 1 cut(s) 1593
Eam1104I CTCTTC 1 cut(s) 1473
Eam1105I GACNNNNNGTC 1 cut(s) 898
EarI CTCTTC 1 cut(s) 1473
EciI GGCGGA 1 cut(s) 1152
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 3 cut(s) 429, 603, 1327
Eco24I GRGCYC 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 1620
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 4 cut(s) 237, 483, 1455, 1707
EcoICRI GAGCTC 1 cut(s) 187
EcoRI GAATTC 1 cut(s) 1515
EcoRII CCWGG 1 cut(s) 177
EcoT14I CCWWGG 3 cut(s) 429, 603, 1327
EcoT22I ATGCAT 1 cut(s) 1573
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 3 cut(s) 429, 603, 1327
Esp3I CGTCTC 2 cut(s) 108, 1719
FalI AAGNNNNNCTT 2 cut(s) 810, 842
FaqI GGGAC 2 cut(s) 1006, 1510
FauI CCCGC 1 cut(s) 250
FblI GTMKAC 1 cut(s) 1452
Fnu4HI GCNGC 4 cut(s) 426, 524, 1227, 1242
FokI GGATG 5 cut(s) 254, 1124, 1152, 1222, 1663
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 4 cut(s) 426, 524, 1227, 1242
FspBI CTAG 7 cut(s) 609, 876, 960, 1043, 1247, 1371, 1718
GlaI GCGC 4 cut(s) 413, 654, 666, 1684
GluI GCNGC 4 cut(s) 426, 524, 1227, 1242
GsaI CCCAGC 2 cut(s) 87, 1496
GsuI CTGGAG 3 cut(s) 262, 321, 789
HaeIII GGCC 2 cut(s) 526, 1595
HapII CCGG 4 cut(s) 696, 1541, 1596, 1610
HgaI GACGC 1 cut(s) 830
HhaI GCGC 4 cut(s) 414, 655, 667, 1685
Hin6I GCGC 4 cut(s) 412, 653, 665, 1683
HinP1I GCGC 4 cut(s) 412, 653, 665, 1683
HincII GTYRAC 3 cut(s) 343, 979, 1039
HindII GTYRAC 3 cut(s) 343, 979, 1039
HinfI GANTC 4 cut(s) 570, 580, 857, 865
HpaI GTTAAC 1 cut(s) 1039
HpaII CCGG 4 cut(s) 696, 1541, 1596, 1610
HphI GGTGA 2 cut(s) 445, 538
Hpy166II GTNNAC 6 cut(s) 343, 436, 691, 979, 1039, 1453
Hpy188I TCNGA 8 cut(s) 70, 626, 684, 703, 831, 862, 1337, 1404
Hpy8I GTNNAC 6 cut(s) 343, 436, 691, 979, 1039, 1453
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 6 cut(s) 18, 261, 623, 1244, 1451, 1465
HpyCH4III ACNGT 7 cut(s) 61, 743, 1218, 1524, 1660, 1676, 1732
HpyCH4IV ACGT 1 cut(s) 687
HpyCH4V TGCA 8 cut(s) 8, 153, 923, 967, 1087, 1094, 1571, 1735
HpyF10VI GCNNNNNNNGC 8 cut(s) 18, 41, 50, 523, 758, 803, 1074, 1388
HpyF3I CTNAG 7 cut(s) 45, 623, 702, 793, 830, 1271, 1583
HpySE526I ACGT 1 cut(s) 687
HspAI GCGC 4 cut(s) 412, 653, 665, 1683
Kpn2I TCCGGA 1 cut(s) 1540
KspAI GTTAAC 1 cut(s) 1039
LmnI GCTCC 5 cut(s) 179, 302, 1195, 1238, 1253
Lsp1109I GCAGC 2 cut(s) 437, 1213
LweI GCATC 9 cut(s) 74, 207, 828, 1081, 1130, 1271, 1558, 1580, 1641
MaeI CTAG 7 cut(s) 609, 876, 960, 1043, 1247, 1371, 1718
MaeII ACGT 1 cut(s) 687
MaeIII GTNAC 3 cut(s) 229, 1670, 1726
MbiI CCGCTC 2 cut(s) 1241, 1256
MboII GAAGA 8 cut(s) 304, 476, 712, 718, 830, 1399, 1480, 1490
MflI RGATCY 5 cut(s) 449, 698, 800, 1332, 1399
MhlI GDGCHC 2 cut(s) 189, 288
MluCI AATT 4 cut(s) 330, 1025, 1515, 1554
MlyI GAGTC 2 cut(s) 574, 859
MmeI TCCRAC 1 cut(s) 1200
Mph1103I ATGCAT 1 cut(s) 1573
MroI TCCGGA 1 cut(s) 1540
MroXI GAANNNNTTC 1 cut(s) 468
MseI TTAA 4 cut(s) 329, 872, 1038, 1604
MslI CAYNNNNRTG 3 cut(s) 153, 407, 1382
MspA1I CMGCKG 4 cut(s) 83, 257, 577, 767
MspI CCGG 4 cut(s) 696, 1541, 1596, 1610
MspR9I CCNGG 3 cut(s) 179, 1597, 1611
MvaI CCWGG 1 cut(s) 179
MvnI CGCG 2 cut(s) 653, 665
MwoI GCNNNNNNNGC 8 cut(s) 18, 41, 50, 523, 758, 803, 1074, 1388
NciI CCSGG 2 cut(s) 1597, 1611
NcoI CCATGG 1 cut(s) 603
NheI GCTAGC 1 cut(s) 608
NlaIV GGNNCC 2 cut(s) 175, 1334
NmuCI GTSAC 2 cut(s) 229, 1670
NsiI ATGCAT 1 cut(s) 1573
NspI RCATGY 2 cut(s) 12, 295
PaeI GCATGC 1 cut(s) 12
PagI TCATGA 1 cut(s) 1173
PciI ACATGT 1 cut(s) 291
PdmI GAANNNNTTC 1 cut(s) 468
PfeI GAWTC 2 cut(s) 570, 857
PflMI CCANNNNNTGG 1 cut(s) 1492
PkrI GCNGC 4 cut(s) 427, 525, 1228, 1243
PleI GAGTC 2 cut(s) 574, 859
PpsI GAGTC 2 cut(s) 574, 859
PscI ACATGT 1 cut(s) 291
PshBI ATTAAT 1 cut(s) 329
Psp124BI GAGCTC 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 177
PspFI CCCAGC 2 cut(s) 83, 1492
PspGI CCWGG 1 cut(s) 177
PspN4I GGNNCC 2 cut(s) 175, 1334
PspPI GGNCC 1 cut(s) 525
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 5 cut(s) 449, 698, 800, 1332, 1399
PvuII CAGCTG 2 cut(s) 577, 767
RsaI GTAC 2 cut(s) 621, 1202
RsaNI GTAC 2 cut(s) 620, 1201
RseI CAYNNNNRTG 3 cut(s) 153, 407, 1382
SacI GAGCTC 1 cut(s) 189
SaqAI TTAA 4 cut(s) 329, 872, 1038, 1604
SatI GCNGC 4 cut(s) 426, 524, 1227, 1242
Sau96I GGNCC 1 cut(s) 525
SchI GAGTC 2 cut(s) 574, 859
ScrFI CCNGG 3 cut(s) 179, 1597, 1611
SduI GDGCHC 2 cut(s) 189, 288
SfaNI GCATC 9 cut(s) 74, 207, 828, 1081, 1130, 1271, 1558, 1580, 1641
SfcI CTRYAG 1 cut(s) 762
SmiMI CAYNNNNRTG 3 cut(s) 153, 407, 1382
SmlI CTYRAG 2 cut(s) 190, 494
SmoI CTYRAG 2 cut(s) 190, 494
SphI GCATGC 1 cut(s) 12
Sse9I AATT 4 cut(s) 330, 1025, 1515, 1554
SsiI CCGC 8 cut(s) 81, 120, 220, 257, 523, 1137, 1241, 1256
SspI AATATT 1 cut(s) 480
SspMI CTAG 7 cut(s) 609, 876, 960, 1043, 1247, 1371, 1718
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 3 cut(s) 177, 1595, 1609
StyI CCWWGG 3 cut(s) 429, 603, 1327
TaaI ACNGT 7 cut(s) 61, 743, 1218, 1524, 1660, 1676, 1732
TaiI ACGT 1 cut(s) 690
TaqI TCGA 2 cut(s) 916, 1566
TasI AATT 4 cut(s) 330, 1025, 1515, 1554
TatI WGTACW 2 cut(s) 619, 1200
TauI GCSGC 2 cut(s) 526, 1244
TfiI GAWTC 2 cut(s) 570, 857
Tru1I TTAA 4 cut(s) 329, 872, 1038, 1604
Tru9I TTAA 4 cut(s) 329, 872, 1038, 1604
TscAI CASTG 7 cut(s) 160, 286, 423, 1053, 1085, 1516, 1737
TseFI GTSAC 2 cut(s) 229, 1670
TseI GCWGC 2 cut(s) 425, 1226
Tsp45I GTSAC 2 cut(s) 229, 1670
TspDTI ATGAA 5 cut(s) 258, 335, 549, 605, 1226
TspGWI ACGGA 1 cut(s) 897
TspRI CASTG 7 cut(s) 160, 286, 423, 1053, 1085, 1516, 1737
Van91I CCANNNNNTGG 1 cut(s) 1492
VspI ATTAAT 1 cut(s) 329
XapI RAATTY 2 cut(s) 1025, 1515
XbaI TCTAGA 1 cut(s) 1717
XceI RCATGY 2 cut(s) 12, 295
XmiI GTMKAC 1 cut(s) 1452
XmnI GAANNNNTTC 1 cut(s) 468
XspI CTAG 7 cut(s) 609, 876, 960, 1043, 1247, 1371, 1718
Zsp2I ATGCAT 1 cut(s) 1573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.