Rw6G027800
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009614
Physical Location & Seq
Forward (+)
51784358 .. 51786073
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G027800.1

Sequence Viewer

Length: 1623 bp
ATGGCTGAGGCAGCATACACAGTGGCATCTGATAGTGAAACCAGTGTGGAGGAGAAATCCTCATGCACCTTTCCTGAAACAACAGTTGGAATTGATATTGGTACCTCACAATGCAGGTTTGCAATCTGGAATGGCTCCCAGGTTGAGATCCTTAAAAACAGTAGGAACCAGAAGATGATGAGTTCATATGTTACGTTCAAGAATGGAACTCCTTCTGGGGGACTAAGCAATCAACTCTCTGCAGATCATGATATATTATCCGGAGCTGCAATATTCAACATGAAACGCTTAATTGGCCGAGTTGACACGGATCCAGTTGTCCACTCTAGCAAAAGCCTTCCATTTCTAGTACAAACATTGGACATTGGTGTCCGTCCATTTATTGCAGCCTTGGTTGGAAATTTCTGGAGATCCACCACTCCTGAAGAAGTTCTGGCAATATTTTTGGTGGAACTTAGGGAAATGGCAGAAATTCAGTTAAGGCGGCCCATAAAAAATGTTTTGACTCGGATTGAGAGAGCATGTGCCATGGCTGGCCTTCATATCCTGAAACTGATGCCAGAACCAACTGCAGTGGCATTGTTATATACCTTATATACAAAACAAAGGGGATTTCAGTCAACAGATAGGAATGTTCTAATCTTCAATATGGGTGCAGGATACTGTGATGTTGCCATAAGTGTGACAGCTGAAGAGGCACCACTAGGAATTTCTGAAATAATTGCCTTAACAGGAAGTGCCATTGGAGGAGAAGACTTGCTTCAGAATATGATGCGTCATCTGTTGCCAAATGCAGAGAATCTTTTGACAAGTCATGGAGTTGACGAAATCAAATCAAAGGGGTTACTTCGAGTTGCAACACAGAATGCAATCCAGAGTCTCTCGTCCCAAAATTCTGTTCAAATTGATGTTGACTTGGGAAATGGGATGAAAATCTGCAAGGTAGTGGACCGAGAAGAATTTGAGGAAGTTAATCGTGCGGTATTTAATAAGTGTGTTAGCCTCATAATCCAGTGTTTGCATGATGCAAAGGTAAAACCTGAGGATGTGAACGATGTAGTACTTAAAGAGGAGCTTTACGAAGGGATGAACCCATTGGAAGCTGCTGTCTCTGGAGCAGCACTACAAGGATACGTAGCTTCAGGTGTTCATGATCCTTTTGAATCCATGGATGTGCTATCTATTCAGATCACCCCTCTCTCGATTGGAATTCGAGCTGATAGAAACAACTTTGTGCCGATCATACCTAGAAACACCACAATACCAGCCCAAAGAGAGATGCTTTTCACAACATCCCATGATAATCAAGCCGAAGCACTTATAATTGTTTATGAAGGTGATGGCAAGAAGCTGGAAGAAAATCATTTGCTGGGATACTGCAAAATTGAGGGAATTCCTCCAGCAACCAAGGGAGTTCCACAAATACGAGTAATGATGGACATTGATGCTTCAAACGTGCTGAGAGTTTTGGCTGCTGTGTTGATGCCAGGTTCTCATCTTCCAATAAATCCTGTCCTGGACGTGAGGATGCCAACGGTTGACGATGGTCATACCTGGTGCGCGGAAGCCTTGCACAGAACTTACGGTACTACTCAAGACTTGGTATCAGTGAAGAGGATCTAG

Protein Analysis

540

Amino Acids

59.07

Weight (kDa)

5.29

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 29 - 494 1.4e-69 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1322
AasI GACNNNNNNGTC 1 cut(s) 368
Acc36I ACCTGC 1 cut(s) 105
Acc65I GGTACC 1 cut(s) 101
AccB1I GGYRCC 2 cut(s) 101, 697
AccII CGCG 1 cut(s) 1562
AccIII TCCGGA 1 cut(s) 260
AciI CCGC 3 cut(s) 484, 980, 1562
AclWI GGATC 5 cut(s) 142, 305, 318, 405, 1148
AcoI YGGCCR 1 cut(s) 295
AcsI RAATTY 7 cut(s) 400, 471, 708, 892, 959, 1209, 1392
AcuI CTGAAG 4 cut(s) 444, 711, 746, 1125
AfaI GTAC 4 cut(s) 103, 351, 1062, 1588
AfiI CCNNNNNNNGG 1 cut(s) 218
AgsI TTSAA 6 cut(s) 199, 277, 646, 902, 1163, 1452
AjiI CACGTC 1 cut(s) 1522
AjnI CCWGG 4 cut(s) 138, 1486, 1515, 1553
AjuI GAANNNNNNNTTGG 4 cut(s) 69, 101, 882, 914
AluBI AGCT 7 cut(s) 266, 689, 1075, 1103, 1139, 1217, 1351
AluI AGCT 7 cut(s) 266, 689, 1075, 1103, 1139, 1217, 1351
Alw26I GTCTC 2 cut(s) 884, 1114
AlwI GGATC 5 cut(s) 142, 305, 318, 405, 1148
Aor13HI TCCGGA 1 cut(s) 260
AoxI GGCC 3 cut(s) 295, 485, 535
ApeKI GCWGC 6 cut(s) 11, 266, 386, 1103, 1118, 1472
ApoI RAATTY 7 cut(s) 400, 471, 708, 892, 959, 1209, 1392
Asp700I GAANNNNTTC 2 cut(s) 211, 429
Asp718I GGTACC 1 cut(s) 101
AspLEI GCGC 1 cut(s) 1562
AspS9I GGNCC 2 cut(s) 486, 949
AsuHPI GGTGA 2 cut(s) 1183, 1349
AvaII GGWCC 1 cut(s) 949
AxyI CCTNAGG 1 cut(s) 1041
BamHI GGATCC 1 cut(s) 310
BanI GGYRCC 2 cut(s) 101, 697
BbsI GAAGAC 1 cut(s) 759
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 6 cut(s) 23, 253, 398, 1090, 1130, 1459
BccI CCATC 3 cut(s) 1334, 1429, 1538
BciT130I CCWGG 4 cut(s) 140, 1488, 1517, 1555
BciVI GTATCC 3 cut(s) 653, 1124, 1367
BcoDI GTCTC 2 cut(s) 884, 1114
BfaI CTAG 5 cut(s) 327, 347, 704, 1248, 1621
BfmI CTRYAG 2 cut(s) 240, 570
BfuAI ACCTGC 1 cut(s) 105
BfuI GTATCC 3 cut(s) 653, 1124, 1367
BisI GCNGC 7 cut(s) 12, 267, 387, 485, 1104, 1119, 1473
BlsI GCNGC 7 cut(s) 13, 268, 388, 486, 1105, 1120, 1474
BmcAI AGTACT 1 cut(s) 1062
Bme1390I CCNGG 4 cut(s) 140, 1488, 1517, 1555
Bme18I GGWCC 1 cut(s) 949
BmgBI CACGTC 1 cut(s) 1522
BmgT120I GGNCC 2 cut(s) 486, 949
BmiI GGNNCC 5 cut(s) 103, 136, 167, 312, 699
BmrFI CCNGG 4 cut(s) 140, 1488, 1517, 1555
BmsI GCATC 8 cut(s) 35, 546, 762, 1015, 1269, 1435, 1473, 1518
BoxI GACNNNNGTC 1 cut(s) 1545
BpiI GAAGAC 1 cut(s) 759
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpmI CTGGAG 3 cut(s) 427, 1134, 1383
Bpu10I CCTNAGC 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 1578
BsaAI YACGTR 1 cut(s) 1135
BsaBI GATNNNNATC 1 cut(s) 932
BsaJI CCNNGG 5 cut(s) 138, 390, 528, 1167, 1407
BsaWI WCCGGW 1 cut(s) 260
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse1I ACTGG 3 cut(s) 42, 314, 1012
Bse21I CCTNAGG 1 cut(s) 1041
Bse8I GATNNNNATC 1 cut(s) 932
BseAI TCCGGA 1 cut(s) 260
BseBI CCWGG 4 cut(s) 140, 1488, 1517, 1555
BseDI CCNNGG 5 cut(s) 138, 390, 528, 1167, 1407
BseGI GGATG 6 cut(s) 933, 1051, 1092, 1177, 1292, 1533
BseJI GATNNNNATC 1 cut(s) 932
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMII CTCAG 2 cut(s) 1032, 1451
BseNI ACTGG 3 cut(s) 42, 314, 1012
BseRI GAGGAG 3 cut(s) 65, 762, 1085
BseXI GCAGC 6 cut(s) 23, 253, 398, 1090, 1130, 1459
BseYI CCCAGC 1 cut(s) 1369
BsgI GTGCAG 1 cut(s) 675
Bsh1236I CGCG 1 cut(s) 1562
BshFI GGCC 3 cut(s) 297, 487, 537
BshNI GGYRCC 2 cut(s) 101, 697
BsiSI CCGG 1 cut(s) 261
BslFI GGGAC 2 cut(s) 234, 871
BslI CCNNNNNNNGG 1 cut(s) 218
BsmAI GTCTC 2 cut(s) 884, 1114
BsmFI GGGAC 2 cut(s) 234, 871
BsmI GAATGC 1 cut(s) 871
BsnI GGCC 3 cut(s) 297, 487, 537
Bsp13I TCCGGA 1 cut(s) 260
Bsp143I GATC 8 cut(s) 147, 244, 310, 410, 1153, 1188, 1239, 1617
Bsp19I CCATGG 2 cut(s) 528, 1167
BspACI CCGC 3 cut(s) 484, 980, 1562
BspANI GGCC 3 cut(s) 297, 487, 537
BspCNI CTCAG 2 cut(s) 1033, 1452
BspEI TCCGGA 1 cut(s) 260
BspFNI CGCG 1 cut(s) 1562
BspHI TCATGA 2 cut(s) 247, 1150
BspLI GGNNCC 5 cut(s) 103, 136, 167, 312, 699
BspMAI CTGCAG 2 cut(s) 244, 574
BspMI ACCTGC 1 cut(s) 105
BspPI GGATC 5 cut(s) 142, 305, 318, 405, 1148
BspT107I GGYRCC 2 cut(s) 101, 697
BsrI ACTGG 3 cut(s) 42, 314, 1012
BssECI CCNNGG 5 cut(s) 138, 390, 528, 1167, 1407
BssMI GATC 8 cut(s) 147, 244, 310, 410, 1153, 1188, 1239, 1617
BssT1I CCWWGG 4 cut(s) 390, 528, 1167, 1407
Bst2UI CCWGG 4 cut(s) 140, 1488, 1517, 1555
Bst4CI ACNGT 6 cut(s) 22, 85, 161, 665, 1537, 1586
Bst6I CTCTTC 2 cut(s) 687, 1607
BstBAI YACGTR 1 cut(s) 1135
BstC8I GCNNGC 1 cut(s) 535
BstDEI CTNAG 5 cut(s) 6, 224, 455, 1041, 1460
BstDSI CCRYGG 2 cut(s) 528, 1167
BstF5I GGATG 6 cut(s) 933, 1051, 1092, 1177, 1292, 1533
BstFNI CGCG 1 cut(s) 1562
BstHHI GCGC 1 cut(s) 1562
BstKTI GATC 8 cut(s) 150, 247, 313, 413, 1156, 1191, 1242, 1620
BstMAI GTCTC 2 cut(s) 884, 1114
BstMBI GATC 8 cut(s) 147, 244, 310, 410, 1153, 1188, 1239, 1617
BstMWI GCNNNNNNNGC 3 cut(s) 11, 294, 695
BstNI CCWGG 4 cut(s) 140, 1488, 1517, 1555
BstNSI RCATGY 1 cut(s) 525
BstPAI GACNNNNGTC 1 cut(s) 1545
BstSCI CCNGG 4 cut(s) 138, 1486, 1515, 1553
BstSFI CTRYAG 2 cut(s) 240, 570
BstSNI TACGTA 1 cut(s) 1135
BstUI CGCG 1 cut(s) 1562
BstV1I GCAGC 6 cut(s) 23, 253, 398, 1090, 1130, 1459
BstV2I GAAGAC 1 cut(s) 759
BstX2I RGATCY 4 cut(s) 147, 310, 410, 1617
BstYI RGATCY 4 cut(s) 147, 310, 410, 1617
Bsu36I CCTNAGG 1 cut(s) 1041
BsuI GTATCC 3 cut(s) 653, 1124, 1367
BsuRI GGCC 3 cut(s) 297, 487, 537
BtgI CCRYGG 2 cut(s) 528, 1167
BtrI CACGTC 1 cut(s) 1522
BtsCI GGATG 6 cut(s) 933, 1051, 1092, 1177, 1292, 1533
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 5 cut(s) 27, 49, 579, 1019, 1614
BveI ACCTGC 1 cut(s) 105
Cac8I GCNNGC 1 cut(s) 535
CciI TCATGA 2 cut(s) 247, 1150
CfoI GCGC 1 cut(s) 1562
Cfr13I GGNCC 2 cut(s) 486, 949
CseI GACGC 1 cut(s) 764
CsiI ACCWGGT 1 cut(s) 1553
Csp6I GTAC 4 cut(s) 102, 350, 1061, 1587
CspCI CAANNNNNGTGG 2 cut(s) 555, 590
CviQI GTAC 4 cut(s) 102, 350, 1061, 1587
DdeI CTNAG 5 cut(s) 6, 224, 455, 1041, 1460
DpnI GATC 8 cut(s) 149, 246, 312, 412, 1155, 1190, 1241, 1619
DpnII GATC 8 cut(s) 147, 244, 310, 410, 1153, 1188, 1239, 1617
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 1 cut(s) 295
Eam1104I CTCTTC 2 cut(s) 687, 1607
EarI CTCTTC 2 cut(s) 687, 1607
Eco105I TACGTA 1 cut(s) 1135
Eco130I CCWWGG 4 cut(s) 390, 528, 1167, 1407
Eco47I GGWCC 1 cut(s) 949
Eco57I CTGAAG 4 cut(s) 444, 711, 746, 1125
Eco81I CCTNAGG 1 cut(s) 1041
EcoRI GAATTC 2 cut(s) 1209, 1392
EcoRII CCWGG 4 cut(s) 138, 1486, 1515, 1553
EcoT14I CCWWGG 4 cut(s) 390, 528, 1167, 1407
ErhI CCWWGG 4 cut(s) 390, 528, 1167, 1407
FalI AAGNNNNNCTT 4 cut(s) 744, 776, 1059, 1091
FaqI GGGAC 2 cut(s) 234, 871
FauNDI CATATG 1 cut(s) 187
Fnu4HI GCNGC 7 cut(s) 12, 267, 387, 485, 1104, 1119, 1473
FokI GGATG 6 cut(s) 940, 1058, 1099, 1184, 1279, 1540
Fsp4HI GCNGC 7 cut(s) 12, 267, 387, 485, 1104, 1119, 1473
FspBI CTAG 5 cut(s) 327, 347, 704, 1248, 1621
GlaI GCGC 1 cut(s) 1561
GluI GCNGC 7 cut(s) 12, 267, 387, 485, 1104, 1119, 1473
GsaI CCCAGC 1 cut(s) 1373
GsuI CTGGAG 3 cut(s) 427, 1134, 1383
HaeIII GGCC 3 cut(s) 297, 487, 537
HapII CCGG 1 cut(s) 261
HgaI GACGC 1 cut(s) 764
HhaI GCGC 1 cut(s) 1562
Hin6I GCGC 1 cut(s) 1560
HinP1I GCGC 1 cut(s) 1560
HincII GTYRAC 5 cut(s) 304, 621, 823, 913, 1540
HindII GTYRAC 5 cut(s) 304, 621, 823, 913, 1540
HinfI GANTC 4 cut(s) 505, 799, 877, 1163
HpaII CCGG 1 cut(s) 261
HphI GGTGA 2 cut(s) 1183, 1349
Hpy166II GTNNAC 8 cut(s) 304, 322, 621, 823, 913, 949, 1051, 1540
Hpy188I TCNGA 5 cut(s) 31, 510, 715, 765, 1188
Hpy8I GTNNAC 8 cut(s) 304, 322, 621, 823, 913, 949, 1051, 1540
HpyAV CCTTC 5 cut(s) 222, 347, 548, 1076, 1328
HpyCH4III ACNGT 6 cut(s) 22, 85, 161, 665, 1537, 1586
HpyCH4IV ACGT 4 cut(s) 194, 1134, 1455, 1521
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 294, 695
HpyF3I CTNAG 5 cut(s) 6, 224, 455, 1041, 1460
HpySE526I ACGT 4 cut(s) 194, 1134, 1455, 1521
HspAI GCGC 1 cut(s) 1560
Kpn2I TCCGGA 1 cut(s) 260
KpnI GGTACC 1 cut(s) 105
Kzo9I GATC 8 cut(s) 147, 244, 310, 410, 1153, 1188, 1239, 1617
LmnI GCTCC 4 cut(s) 140, 263, 1072, 1115
Lsp1109I GCAGC 6 cut(s) 23, 253, 398, 1090, 1130, 1459
LweI GCATC 8 cut(s) 35, 546, 762, 1015, 1269, 1435, 1473, 1518
MabI ACCWGGT 1 cut(s) 1553
MaeI CTAG 5 cut(s) 327, 347, 704, 1248, 1621
MaeII ACGT 4 cut(s) 194, 1134, 1455, 1521
MaeIII GTNAC 3 cut(s) 190, 682, 843
MalI GATC 8 cut(s) 149, 246, 312, 412, 1155, 1190, 1241, 1619
MboI GATC 8 cut(s) 147, 244, 310, 410, 1153, 1188, 1239, 1617
MboII GAAGA 8 cut(s) 184, 437, 634, 704, 764, 968, 1367, 1490
MflI RGATCY 4 cut(s) 147, 310, 410, 1617
MlyI GAGTC 2 cut(s) 499, 886
MmeI TCCRAC 2 cut(s) 67, 376
MroI TCCGGA 1 cut(s) 260
MroXI GAANNNNTTC 2 cut(s) 211, 429
MseI TTAA 7 cut(s) 153, 290, 479, 728, 972, 987, 1065
MslI CAYNNNNRTG 2 cut(s) 680, 1172
MspA1I CMGCKG 1 cut(s) 689
MspI CCGG 1 cut(s) 261
MspR9I CCNGG 4 cut(s) 140, 1488, 1517, 1555
Mva1269I GAATGC 1 cut(s) 871
MvaI CCWGG 4 cut(s) 140, 1488, 1517, 1555
MvnI CGCG 1 cut(s) 1562
MwoI GCNNNNNNNGC 3 cut(s) 11, 294, 695
NcoI CCATGG 2 cut(s) 528, 1167
NdeI CATATG 1 cut(s) 187
NdeII GATC 8 cut(s) 147, 244, 310, 410, 1153, 1188, 1239, 1617
NlaIV GGNNCC 5 cut(s) 103, 136, 167, 312, 699
NmeAIII GCCGAG 1 cut(s) 323
NmuCI GTSAC 1 cut(s) 682
NspI RCATGY 1 cut(s) 525
PagI TCATGA 2 cut(s) 247, 1150
PctI GAATGC 1 cut(s) 871
PdmI GAANNNNTTC 2 cut(s) 211, 429
PfeI GAWTC 2 cut(s) 799, 1163
PfoI TCCNGGA 1 cut(s) 1515
PkrI GCNGC 7 cut(s) 13, 268, 388, 486, 1105, 1120, 1474
PleI GAGTC 2 cut(s) 499, 885
PpsI GAGTC 2 cut(s) 499, 885
Ppu21I YACGTR 1 cut(s) 1135
PshAI GACNNNNGTC 1 cut(s) 1545
PsiI TTATAA 1 cut(s) 1322
Psp6I CCWGG 4 cut(s) 138, 1486, 1515, 1553
PspFI CCCAGC 1 cut(s) 1369
PspGI CCWGG 4 cut(s) 138, 1486, 1515, 1553
PspN4I GGNNCC 5 cut(s) 103, 136, 167, 312, 699
PspPI GGNCC 2 cut(s) 486, 949
PsrI GAACNNNNNNTAC 2 cut(s) 1570, 1602
PstI CTGCAG 2 cut(s) 244, 574
PsuI RGATCY 4 cut(s) 147, 310, 410, 1617
PvuII CAGCTG 1 cut(s) 689
RsaI GTAC 4 cut(s) 103, 351, 1062, 1588
RsaNI GTAC 4 cut(s) 102, 350, 1061, 1587
RseI CAYNNNNRTG 2 cut(s) 680, 1172
SaqAI TTAA 7 cut(s) 153, 290, 479, 728, 972, 987, 1065
SatI GCNGC 7 cut(s) 12, 267, 387, 485, 1104, 1119, 1473
Sau3AI GATC 8 cut(s) 147, 244, 310, 410, 1153, 1188, 1239, 1617
Sau96I GGNCC 2 cut(s) 486, 949
ScaI AGTACT 1 cut(s) 1062
SchI GAGTC 2 cut(s) 499, 886
ScrFI CCNGG 4 cut(s) 140, 1488, 1517, 1555
SexAI ACCWGGT 1 cut(s) 1553
SfaNI GCATC 8 cut(s) 35, 546, 762, 1015, 1269, 1435, 1473, 1518
SfcI CTRYAG 2 cut(s) 240, 570
SinI GGWCC 1 cut(s) 949
SmiMI CAYNNNNRTG 2 cut(s) 680, 1172
SmlI CTYRAG 1 cut(s) 1593
SmoI CTYRAG 1 cut(s) 1593
SnaBI TACGTA 1 cut(s) 1135
SsiI CCGC 3 cut(s) 484, 980, 1562
SspI AATATT 2 cut(s) 273, 441
SspMI CTAG 5 cut(s) 327, 347, 704, 1248, 1621
StyD4I CCNGG 4 cut(s) 138, 1486, 1515, 1553
StyI CCWWGG 4 cut(s) 390, 528, 1167, 1407
TaaI ACNGT 6 cut(s) 22, 85, 161, 665, 1537, 1586
TaiI ACGT 4 cut(s) 197, 1137, 1458, 1524
TaqI TCGA 3 cut(s) 850, 1202, 1213
TaqII GACCGA 1 cut(s) 966
TatI WGTACW 2 cut(s) 349, 1060
TauI GCSGC 1 cut(s) 487
TfiI GAWTC 2 cut(s) 799, 1163
Tru1I TTAA 7 cut(s) 153, 290, 479, 728, 972, 987, 1065
Tru9I TTAA 7 cut(s) 153, 290, 479, 728, 972, 987, 1065
TscAI CASTG 5 cut(s) 27, 49, 579, 1019, 1614
TseFI GTSAC 1 cut(s) 682
TseI GCWGC 6 cut(s) 11, 266, 386, 1103, 1118, 1472
Tsp45I GTSAC 1 cut(s) 682
TspDTI ATGAA 7 cut(s) 174, 296, 530, 944, 1103, 1139, 1347
TspGWI ACGGA 2 cut(s) 323, 362
TspRI CASTG 5 cut(s) 27, 49, 579, 1019, 1614
VpaK11BI GGWCC 1 cut(s) 949
XapI RAATTY 7 cut(s) 400, 471, 708, 892, 959, 1209, 1392
XceI RCATGY 1 cut(s) 525
XmnI GAANNNNTTC 2 cut(s) 211, 429
XspI CTAG 5 cut(s) 327, 347, 704, 1248, 1621
ZrmI AGTACT 1 cut(s) 1062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.