MD13G1191900.v1.1
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009614
Physical Location & Seq
Forward (+)
16502036 .. 16505010
2975 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1191900.v1.1.491

Sequence Viewer

Length: 1707 bp
ATGGCTGAGCCAGCATACACAGTGGCATCTGATAGTGAAACCACTGGAGAGGAAAAATCGCCTTCTCCTTTTCCTGAAACCGCTATTGGGATTGACATTGGCACTTCACATTGCAGTGTTGCGGTCTGGAACGGCTCCCAGGTTGAGCTCCTTAGGAACAGTAGAAACCAGAAAATGATGCGGTCGTATGTCACATTCAAGAATGAAAACCCTTCAGGAGGAGTAAGCTATCAACTCTCCAGTGAGCACGAGATGTTATCTGGAGCTTCAATTTTCAACATGAAACGATTAATTGGCAGGGTTGACACTGATCCAGTTGTCCAGTCAAGCAAAAGCCTCCCCTTTTTAGTACAAACATTGGGCATTGGGGTGCGCCCACTGATCGCAGCCTTGGTGAACAATGTATGGAGATCCACCACTCCTGAAGAAGTTCTGGCAATATTCTTGGTGGAACTCAAGGCAATGGCAGAAATCGAGCTAAATCGACCCATAAAAAATGTTGTTCTGACTATTCCAGCTTCATTCAGCCGATTCCAGCTTACTCGTATTGAAAGAGCTTGTGCCATGGCTAGCCTTCATGTACTCAGACTGATGCCTGAACCAACTGCCGTCGCACTGTTATATGCACAAAAGATTTCAAATCAGAACGTAAACACCGGATCTGAGAAGATTGCTCTAATCTTCAACATGGGTGCTGGATACTGCGATGTAGCTGTTACTGCTACAGCTGGAGGAGTTTCCCAAATAAAAGCCTTAGCAGGGTCTGCCATTGGAGGGGAAGACTTGCTTCAGAATATGATGCGTCATCTGTTGCCAGATTCCGAGACTCTTATAACTAGCCACGGACTTGATGACATAAAATCAGCAGGCATTCTTCGAGTTGCAACACAGGAGGCAATCCACAATCTCTCCTCCCAAACAAGTGTTCAGATTGATGCTGACTTGGGAAATGGAACAAATATATGTAAGGTTGTGGATCGTGAGGAATTTGAGGAAGTTAACCGAGCAGTGTTTGATAAGTGCGCGAGCCTCATAAGCCAGTGCTTGCACGACGCAAAGGTTGATACCGAGGATGTGAGCGATGTAATAGTTGTTGGTGGATGCTCATATATTCCAAAGATAAGAAGTCTTGTCATGAGCATTTGTAAAAAGGAGGAGCTGTACAAAGGGATGAACCAGTTGGAAGCTGCTGTCACTGGAGCGGCACTAGAAGGAGCGGTGGCATCAGGTCTTAGCGATCCCTTTGGTAGTTTGGACTTGCTTACCATTCAAGCCACCCCTCTCGCCATTGGGATTCAAGCTGATGGAAACAACTTTGTGCCTATCATACCTAGAAACACAGCAATGCCCGCACAAAAAGATATGATTTTCACAACTGCCCATGATAATCAAACTGAAGCACTAATAATCGTCTACGAAGGCGAGGGGAAGAAGGTGGAAGAGAACCATTTGCTGGGGTATTTCAAGATCACTGGAATTCCACCATCACCGAAAGGAGTTCCGGAAATAAGAATAATTCTGGACATCGATGCATCGAGCGTGCTGAGAGTTTTATCGGGGGTTTTAATGCCGGGGTCTCATCAGCCGGTAAATCCAGTCATGGGAGTGAGGATGCCAACAGTTGATGATGGTCATGGCTGGTGTGCCGAAGCCCTACACAGAACTTATGGTTCTACTCTCGACTTGGTTACAGTGCAGAAGATATAG

Protein Analysis

569

Amino Acids

61.08

Weight (kDa)

5.23

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 28 - 520 1.9e-95 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
AccB7I CCANNNNNTGG 1 cut(s) 1453
AccBSI CCGCTC 2 cut(s) 1202, 1217
AccI GTMKAC 1 cut(s) 1413
AccII CGCG 1 cut(s) 1025
AccIII TCCGGA 1 cut(s) 1501
AciI CCGC 6 cut(s) 81, 122, 181, 1202, 1217, 1350
AclWI GGATC 5 cut(s) 305, 405, 667, 984, 1232
AcsI RAATTY 2 cut(s) 986, 1476
AcuI CTGAAG 4 cut(s) 198, 444, 773, 1416
AfaI GTAC 3 cut(s) 351, 582, 1163
AfiI CCNNNNNNNGG 6 cut(s) 87, 218, 759, 774, 1453, 1601
AgsI TTSAA 9 cut(s) 199, 270, 277, 551, 639, 685, 1271, 1298, 1465
AjnI CCWGG 1 cut(s) 138
AjuI GAANNNNNNNTTGG 4 cut(s) 69, 101, 909, 941
Alw21I GWGCWC 2 cut(s) 150, 249
Alw26I GTCTC 2 cut(s) 818, 1581
AlwI GGATC 5 cut(s) 305, 405, 667, 984, 1232
AlwNI CAGNNNCTG 1 cut(s) 764
Aor13HI TCCGGA 1 cut(s) 1501
ApeKI GCWGC 2 cut(s) 386, 1187
ApoI RAATTY 2 cut(s) 986, 1476
AseI ATTAAT 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 429
AspLEI GCGC 2 cut(s) 375, 1025
AsuC2I CCSGG 1 cut(s) 1572
AsuHPI GGTGA 2 cut(s) 406, 1479
AsuNHI GCTAGC 1 cut(s) 569
AxyI CCTNAGG 1 cut(s) 152
BanII GRGCYC 1 cut(s) 150
BauI CACGAG 1 cut(s) 248
BbsI GAAGAC 1 cut(s) 786
Bbv12I GWGCWC 2 cut(s) 150, 249
BbvI GCAGC 2 cut(s) 398, 1174
BccI CCATC 3 cut(s) 1298, 1492, 1622
BceAI ACGGC 2 cut(s) 148, 593
BciT130I CCWGG 1 cut(s) 140
BciVI GTATCC 1 cut(s) 692
BcnI CCSGG 1 cut(s) 1572
BcoDI GTCTC 2 cut(s) 818, 1581
BfaI CTAG 4 cut(s) 570, 837, 1208, 1332
BfmI CTRYAG 1 cut(s) 723
BfuI GTATCC 1 cut(s) 692
BisI GCNGC 3 cut(s) 387, 1188, 1203
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 3 cut(s) 388, 1189, 1204
Bme1390I CCNGG 2 cut(s) 140, 1572
BmiI GGNNCC 1 cut(s) 136
BmrFI CCNGG 2 cut(s) 140, 1572
BmtI GCTAGC 1 cut(s) 573
BpiI GAAGAC 1 cut(s) 786
BpmI CTGGAG 5 cut(s) 66, 223, 282, 750, 1218
Bpu10I CCTNAGC 1 cut(s) 754
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 440
BpuMI CCSGG 1 cut(s) 1572
Bsa29I ATCGAT 1 cut(s) 1527
BsaI GGTCTC 1 cut(s) 1581
BsaJI CCNNGG 6 cut(s) 138, 390, 564, 841, 1068, 1571
BsaWI WCCGGW 2 cut(s) 656, 1501
BsaXI ACNNNNNCTCC 4 cut(s) 893, 923, 1145, 1175
Bsc4I CCNNNNNNNGG 6 cut(s) 87, 218, 759, 774, 1453, 1601
Bse118I RCCGGY 1 cut(s) 1585
Bse1I ACTGG 9 cut(s) 49, 240, 314, 322, 1039, 1177, 1201, 1477, 1595
Bse21I CCTNAGG 1 cut(s) 152
Bse3DI GCAATG 3 cut(s) 109, 468, 1350
BseAI TCCGGA 1 cut(s) 1501
BseBI CCWGG 1 cut(s) 140
BseCI ATCGAT 1 cut(s) 1527
BseDI CCNNGG 6 cut(s) 138, 390, 564, 841, 1068, 1571
BseGI GGATG 4 cut(s) 1078, 1106, 1176, 1617
BseLI CCNNNNNNNGG 6 cut(s) 87, 218, 759, 774, 1453, 1601
BseMI GCAATG 3 cut(s) 109, 468, 1350
BseMII CTCAG 3 cut(s) 598, 654, 1535
BseNI ACTGG 9 cut(s) 49, 240, 314, 322, 1039, 1177, 1201, 1477, 1595
BseRI GAGGAG 4 cut(s) 234, 747, 901, 1169
BseXI GCAGC 2 cut(s) 398, 1174
BseYI CCCAGC 1 cut(s) 1453
Bsh1236I CGCG 1 cut(s) 1025
Bsh1285I CGRYCG 1 cut(s) 185
BshVI ATCGAT 1 cut(s) 1527
BsiEI CGRYCG 1 cut(s) 185
BsiHKAI GWGCWC 2 cut(s) 150, 249
BsiSI CCGG 4 cut(s) 657, 1502, 1571, 1586
BslI CCNNNNNNNGG 6 cut(s) 87, 218, 759, 774, 1453, 1601
BsmAI GTCTC 2 cut(s) 818, 1581
BsmI GAATGC 1 cut(s) 870
Bso31I GGTCTC 1 cut(s) 1581
Bsp1286I GDGCHC 2 cut(s) 150, 249
Bsp13I TCCGGA 1 cut(s) 1501
Bsp1407I TGTACA 1 cut(s) 1161
Bsp143I GATC 7 cut(s) 310, 381, 410, 659, 976, 1237, 1467
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 564
BspACI CCGC 6 cut(s) 81, 122, 181, 1202, 1217, 1350
BspCNI CTCAG 3 cut(s) 597, 655, 1536
BspDI ATCGAT 1 cut(s) 1527
BspEI TCCGGA 1 cut(s) 1501
BspFNI CGCG 1 cut(s) 1025
BspHI TCATGA 1 cut(s) 1134
BspLI GGNNCC 1 cut(s) 136
BspOI GCTAGC 1 cut(s) 573
BspPI GGATC 5 cut(s) 305, 405, 667, 984, 1232
BspTNI GGTCTC 1 cut(s) 1581
BsrBI CCGCTC 2 cut(s) 1202, 1217
BsrDI GCAATG 3 cut(s) 109, 468, 1350
BsrFI RCCGGY 1 cut(s) 1585
BsrGI TGTACA 1 cut(s) 1161
BsrI ACTGG 9 cut(s) 49, 240, 314, 322, 1039, 1177, 1201, 1477, 1595
BssAI RCCGGY 1 cut(s) 1585
BssECI CCNNGG 6 cut(s) 138, 390, 564, 841, 1068, 1571
BssMI GATC 7 cut(s) 310, 381, 410, 659, 976, 1237, 1467
BssSI CACGAG 1 cut(s) 248
BssT1I CCWWGG 2 cut(s) 390, 564
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 5 cut(s) 22, 161, 618, 1621, 1693
Bst6I CTCTTC 1 cut(s) 1434
BstAPI GCANNNNNTGC 1 cut(s) 764
BstAUI TGTACA 1 cut(s) 1161
BstC8I GCNNGC 7 cut(s) 12, 571, 868, 1027, 1046, 1350, 1541
BstDEI CTNAG 7 cut(s) 6, 152, 584, 663, 754, 1232, 1544
BstDSI CCRYGG 2 cut(s) 564, 841
BstENI CCTNNNNNAGG 1 cut(s) 216
BstF5I GGATG 4 cut(s) 1078, 1106, 1176, 1617
BstFNI CGCG 1 cut(s) 1025
BstHHI GCGC 2 cut(s) 375, 1025
BstKTI GATC 7 cut(s) 313, 384, 413, 662, 979, 1240, 1470
BstMAI GTCTC 2 cut(s) 818, 1581
BstMBI GATC 7 cut(s) 310, 381, 410, 659, 976, 1237, 1467
BstMCI CGRYCG 1 cut(s) 185
BstMWI GCNNNNNNNGC 5 cut(s) 11, 719, 764, 1035, 1349
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 138, 1570
BstSFI CTRYAG 1 cut(s) 723
BstUI CGCG 1 cut(s) 1025
BstV1I GCAGC 2 cut(s) 398, 1174
BstV2I GAAGAC 1 cut(s) 786
BstX2I RGATCY 2 cut(s) 410, 659
BstYI RGATCY 2 cut(s) 410, 659
Bsu15I ATCGAT 1 cut(s) 1527
Bsu36I CCTNAGG 1 cut(s) 152
BsuI GTATCC 1 cut(s) 692
BsuTUI ATCGAT 1 cut(s) 1527
BtgI CCRYGG 2 cut(s) 564, 841
BtgZI GCGATG 2 cut(s) 720, 1095
BtsCI GGATG 4 cut(s) 1078, 1106, 1176, 1617
BtsI GCAGTG 2 cut(s) 121, 1014
Cac8I GCNNGC 7 cut(s) 12, 571, 868, 1027, 1046, 1350, 1541
CaiI CAGNNNCTG 1 cut(s) 764
CciI TCATGA 1 cut(s) 1134
CfoI GCGC 2 cut(s) 375, 1025
Cfr10I RCCGGY 1 cut(s) 1585
ClaI ATCGAT 1 cut(s) 1527
CseI GACGC 2 cut(s) 791, 1061
Csp6I GTAC 3 cut(s) 350, 581, 1162
CviAII CATG 8 cut(s) 280, 565, 578, 688, 1135, 1382, 1600, 1634
CviQI GTAC 3 cut(s) 350, 581, 1162
DdeI CTNAG 7 cut(s) 6, 152, 584, 663, 754, 1232, 1544
DpnI GATC 7 cut(s) 312, 383, 412, 661, 978, 1239, 1469
DpnII GATC 7 cut(s) 310, 381, 410, 659, 976, 1237, 1467
Eam1104I CTCTTC 1 cut(s) 1434
EarI CTCTTC 1 cut(s) 1434
Ecl136II GAGCTC 1 cut(s) 148
Eco130I CCWWGG 2 cut(s) 390, 564
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 1581
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 4 cut(s) 198, 444, 773, 1416
Eco81I CCTNAGG 1 cut(s) 152
EcoICRI GAGCTC 1 cut(s) 148
EcoNI CCTNNNNNAGG 1 cut(s) 216
EcoRI GAATTC 1 cut(s) 1476
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 2 cut(s) 390, 564
EcoT22I ATGCAT 1 cut(s) 1534
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 2 cut(s) 390, 564
FaeI CATG 8 cut(s) 283, 568, 581, 691, 1138, 1385, 1603, 1637
FalI AAGNNNNNCTT 2 cut(s) 771, 803
FatI CATG 8 cut(s) 279, 564, 577, 687, 1134, 1381, 1599, 1633
FauI CCCGC 1 cut(s) 1357
FblI GTMKAC 1 cut(s) 1413
Fnu4HI GCNGC 3 cut(s) 387, 1188, 1203
FokI GGATG 4 cut(s) 1085, 1113, 1183, 1624
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 3 cut(s) 387, 1188, 1203
FspBI CTAG 4 cut(s) 570, 837, 1208, 1332
GlaI GCGC 2 cut(s) 374, 1024
GluI GCNGC 3 cut(s) 387, 1188, 1203
GsaI CCCAGC 1 cut(s) 1457
GsuI CTGGAG 5 cut(s) 66, 223, 282, 750, 1218
HapII CCGG 4 cut(s) 657, 1502, 1571, 1586
HgaI GACGC 2 cut(s) 791, 1061
HhaI GCGC 2 cut(s) 375, 1025
Hin1II CATG 8 cut(s) 283, 568, 581, 691, 1138, 1385, 1603, 1637
Hin6I GCGC 2 cut(s) 373, 1023
HinP1I GCGC 2 cut(s) 373, 1023
HincII GTYRAC 2 cut(s) 304, 1000
HindII GTYRAC 2 cut(s) 304, 1000
HinfI GANTC 4 cut(s) 531, 818, 826, 1294
HpaI GTTAAC 1 cut(s) 1000
HpaII CCGG 4 cut(s) 657, 1502, 1571, 1586
HphI GGTGA 2 cut(s) 406, 1479
Hpy166II GTNNAC 5 cut(s) 304, 397, 652, 1000, 1414
Hpy188I TCNGA 8 cut(s) 31, 507, 587, 645, 664, 792, 823, 930
Hpy8I GTNNAC 5 cut(s) 304, 397, 652, 1000, 1414
Hpy99I CGWCG 2 cut(s) 614, 1055
HpyAV CCTTC 6 cut(s) 72, 222, 584, 1205, 1412, 1426
HpyCH4III ACNGT 5 cut(s) 22, 161, 618, 1621, 1693
HpyCH4IV ACGT 1 cut(s) 648
HpyCH4V TGCA 6 cut(s) 114, 626, 884, 1048, 1532, 1696
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 719, 764, 1035, 1349
HpyF3I CTNAG 7 cut(s) 6, 152, 584, 663, 754, 1232, 1544
HpySE526I ACGT 1 cut(s) 648
Hsp92II CATG 8 cut(s) 283, 568, 581, 691, 1138, 1385, 1603, 1637
HspAI GCGC 2 cut(s) 373, 1023
Kpn2I TCCGGA 1 cut(s) 1501
KspAI GTTAAC 1 cut(s) 1000
Kzo9I GATC 7 cut(s) 310, 381, 410, 659, 976, 1237, 1467
LmnI GCTCC 6 cut(s) 140, 153, 263, 1156, 1199, 1214
Lsp1109I GCAGC 2 cut(s) 398, 1174
MaeI CTAG 4 cut(s) 570, 837, 1208, 1332
MaeII ACGT 1 cut(s) 648
MaeIII GTNAC 4 cut(s) 190, 715, 1192, 1687
MalI GATC 7 cut(s) 312, 383, 412, 661, 978, 1239, 1469
MbiI CCGCTC 2 cut(s) 1202, 1217
MboI GATC 7 cut(s) 310, 381, 410, 659, 976, 1237, 1467
MboII GAAGA 7 cut(s) 437, 673, 679, 791, 866, 1441, 1451
MflI RGATCY 2 cut(s) 410, 659
MhlI GDGCHC 2 cut(s) 150, 249
MluCI AATT 5 cut(s) 270, 291, 986, 1476, 1515
MlyI GAGTC 1 cut(s) 820
MmeI TCCRAC 1 cut(s) 1161
Mph1103I ATGCAT 1 cut(s) 1534
MroI TCCGGA 1 cut(s) 1501
MroXI GAANNNNTTC 1 cut(s) 429
MseI TTAA 3 cut(s) 290, 999, 1565
MslI CAYNNNNRTG 4 cut(s) 114, 368, 1343, 1604
MspA1I CMGCKG 1 cut(s) 728
MspI CCGG 4 cut(s) 657, 1502, 1571, 1586
MspR9I CCNGG 2 cut(s) 140, 1572
Mva1269I GAATGC 1 cut(s) 870
MvaI CCWGG 1 cut(s) 140
MvnI CGCG 1 cut(s) 1025
MwoI GCNNNNNNNGC 5 cut(s) 11, 719, 764, 1035, 1349
NciI CCSGG 1 cut(s) 1572
NcoI CCATGG 1 cut(s) 564
NdeII GATC 7 cut(s) 310, 381, 410, 659, 976, 1237, 1467
NheI GCTAGC 1 cut(s) 569
NlaIII CATG 8 cut(s) 283, 568, 581, 691, 1138, 1385, 1603, 1637
NlaIV GGNNCC 1 cut(s) 136
NmuCI GTSAC 2 cut(s) 190, 1192
NsiI ATGCAT 1 cut(s) 1534
PagI TCATGA 1 cut(s) 1134
PctI GAATGC 1 cut(s) 870
PdmI GAANNNNTTC 1 cut(s) 429
PfeI GAWTC 3 cut(s) 531, 818, 1294
PflMI CCANNNNNTGG 1 cut(s) 1453
PkrI GCNGC 3 cut(s) 388, 1189, 1204
PleI GAGTC 1 cut(s) 820
PpsI GAGTC 1 cut(s) 820
PshBI ATTAAT 1 cut(s) 290
PsiI TTATAA 1 cut(s) 833
Psp124BI GAGCTC 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 138
PspFI CCCAGC 1 cut(s) 1453
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 1 cut(s) 136
PstNI CAGNNNCTG 1 cut(s) 764
PsuI RGATCY 2 cut(s) 410, 659
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 3 cut(s) 351, 582, 1163
RsaNI GTAC 3 cut(s) 350, 581, 1162
RseI CAYNNNNRTG 4 cut(s) 114, 368, 1343, 1604
SacI GAGCTC 1 cut(s) 150
SaqAI TTAA 3 cut(s) 290, 999, 1565
SatI GCNGC 3 cut(s) 387, 1188, 1203
Sau3AI GATC 7 cut(s) 310, 381, 410, 659, 976, 1237, 1467
SchI GAGTC 1 cut(s) 820
ScrFI CCNGG 2 cut(s) 140, 1572
SduI GDGCHC 2 cut(s) 150, 249
SfcI CTRYAG 1 cut(s) 723
SmiMI CAYNNNNRTG 4 cut(s) 114, 368, 1343, 1604
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 5 cut(s) 270, 291, 986, 1476, 1515
SsiI CCGC 6 cut(s) 81, 122, 181, 1202, 1217, 1350
SspI AATATT 1 cut(s) 441
SspMI CTAG 4 cut(s) 570, 837, 1208, 1332
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 2 cut(s) 138, 1570
StyI CCWWGG 2 cut(s) 390, 564
TaaI ACNGT 5 cut(s) 22, 161, 618, 1621, 1693
TaiI ACGT 1 cut(s) 651
TaqI TCGA 6 cut(s) 474, 484, 877, 1527, 1535, 1680
TasI AATT 5 cut(s) 270, 291, 986, 1476, 1515
TatI WGTACW 3 cut(s) 349, 580, 1161
TauI GCSGC 1 cut(s) 1205
TfiI GAWTC 3 cut(s) 531, 818, 1294
Tru1I TTAA 3 cut(s) 290, 999, 1565
Tru9I TTAA 3 cut(s) 290, 999, 1565
TseFI GTSAC 2 cut(s) 190, 1192
TseI GCWGC 2 cut(s) 386, 1187
Tsp45I GTSAC 2 cut(s) 190, 1192
TspDTI ATGAA 5 cut(s) 219, 296, 510, 566, 1187
TspGWI ACGGA 1 cut(s) 858
Van91I CCANNNNNTGG 1 cut(s) 1453
VspI ATTAAT 1 cut(s) 290
XagI CCTNNNNNAGG 1 cut(s) 216
XapI RAATTY 2 cut(s) 986, 1476
XmiI GTMKAC 1 cut(s) 1413
XmnI GAANNNNTTC 1 cut(s) 429
XspI CTAG 4 cut(s) 570, 837, 1208, 1332
Zsp2I ATGCAT 1 cut(s) 1534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.