FvH4_4g15410
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009614
Physical Location & Seq
Forward (+)
19083943 .. 19087499
3557 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g15410.t1

Sequence Viewer

Length: 1716 bp
ATGGCCGAGGCAGCATACACAGTGGCATCTGATAGTGAAACCAGTCGGGAGGAAAAATCCTCATTCACCTTTCCTGAAACAACAATTGGAATTGATATTGGTACTTCACAATGCAGGGTCGCAATATGGAACGGCTCCCAAGTTGAGATACTGAAAAACAGTAGGAATGTGAAGATGATGAGTTCGTATGTTACATTCAAGGATGGAACCCCTTCAGGGGAAATCAGCAATCCACTCTCTACGGATCATGAAATGTTATCTGGAGCTGCAATCTTCAACATGAAACGCTTAATTGGCCGAGTTGACACGGATCCAGTTGTACACTCTAGCAAAAGCCTTCCATTTCTAGTACAAACATTGGATATTGGTGTCCGCCCATTTATTGCAGCCTTGGTTGGCAATTTCTGGAGATCCACCACTCCTGAAGAAGTTATGGCAATTTATTTGGTGGAACTGAGGGAAATGGTAGAAATCCAATTAAGAAGGCCCATAAAAAATGTTGTTCTAACCGTGCCAGTTTCATTCAGCCGATTCCAGCTTACTCGAATCGAAAGAGCTTGTGCCGTGGCTGGCCTTCATATCCTGAGACTGATGCCAGAACCAACTGCAGTGGCATTGTTATATACCTTGTATGCAAAGCAAAGTGGATTTCAGTCAACAGATAGGAATGTTCTAATCTTCAATATGGGTGCTGGATACTGTGATGTAGCTCTAAGTGTTACAGCTGAAGATGCACCTCGAGGAATTTCTGAAATAATTGCCTTAACAGGAAGTGCCATTGGAGGAGAAGACCTGCTTCAGAATATGATGCGTCATGTGTTGCCAAATGCAGAGAATCTTTTGACAAGTCATGGAGTTGAGGAGATCAAATCAAAGGGGTTACTTCGAGTTGCGACCCAGAATGCAATCCATAGTCTCTCCTCCCAAAGTTCTGTTCAAATTGATGTTGACTTGGGAAATGGGATGAAAATCTGCAAGGTTGTGGACCGGGAAGAATTTGAGGAAGTTAATTGTGCAGTATTTGATAAGTGTGCTAGCCTCATTATCCAGTGTTTGCATGATGCAAAGGTAAAACCCAAGGATGTGAACGATGTGATACTTGTTGGTGGATGTTCATATATTCCAAAAATAAAATATCTTCTCATGAGTATATGTAAAAAAGAACAGCTTTATGAAGGGATAAATCCGCTGGAAGCTGCTGTCTCTGGCGCAGCACTAGAGGGATACATAGCTTCAGGAGTTCATGATCCTTTTGAATCTGTGGATGTGCTATCTATTCAGGTCACACCTCTGTCAATTGGAGTTCGAGCAGATGGAAACAACTTTGTGCCGATCATACCTAGAAACACCAGAATACCAACCCAAAGAGAGATGCTATTCACAACCGCTCATGATAATCAGGTGCAAGCACTTACAGTTGTTTATGAAGGTGATGGCAACAAGCTTGAAGAAAATCATTTGCTGGGATACTTCAAGATAGAGGGAATTCCTCCAGCCCCCAAAGGAGTTCCACAAATACGAGTAATGATGGACATTGACACTTCAAATGTGCTGAGAGTTTTGGCTGCTGTGTTAATGCCTGGTTCTCATCTTCCAATAAATCCTGTCCTGGACGTGAGAATGCCTACAGTTGACGATGGTCATGCCTGGTGCGCGGAAGCCCTGAACAGAACTTATGGTGCTACTCTAGACTTGGTATCAGTGAAGAGGATCTAG

Protein Analysis

572

Amino Acids

62.44

Weight (kDa)

5.49

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 29 - 525 9.4e-93 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 738
Acc36I ACCTGC 1 cut(s) 801
AccBSI CCGCTC 1 cut(s) 1388
AccII CGCG 1 cut(s) 1655
AciI CCGC 4 cut(s) 373, 1187, 1386, 1655
AclWI GGATC 5 cut(s) 252, 305, 318, 405, 1241
AcoI YGGCCR 2 cut(s) 3, 295
AcsI RAATTY 3 cut(s) 744, 995, 1485
AcuI CTGAAG 5 cut(s) 198, 444, 747, 782, 1218
AfaI GTAC 3 cut(s) 103, 321, 351
AfiI CCNNNNNNNGG 2 cut(s) 216, 217
AgsI TTSAA 8 cut(s) 199, 277, 682, 938, 1256, 1448, 1474, 1545
AjiI CACGTC 1 cut(s) 1615
AjnI CCWGG 3 cut(s) 1579, 1608, 1646
AjuI GAANNNNNNNTTGG 4 cut(s) 69, 101, 918, 950
AluBI AGCT 9 cut(s) 266, 538, 557, 710, 725, 1168, 1196, 1232, 1444
AluI AGCT 9 cut(s) 266, 538, 557, 710, 725, 1168, 1196, 1232, 1444
Alw26I GTCTC 3 cut(s) 580, 920, 1207
AlwI GGATC 5 cut(s) 252, 305, 318, 405, 1241
Ama87I CYCGRG 1 cut(s) 738
AoxI GGCC 4 cut(s) 3, 295, 485, 571
ApeKI GCWGC 6 cut(s) 11, 266, 386, 1196, 1211, 1565
ApoI RAATTY 3 cut(s) 744, 995, 1485
Asp700I GAANNNNTTC 1 cut(s) 211
AspLEI GCGC 2 cut(s) 1211, 1655
AspS9I GGNCC 2 cut(s) 486, 985
AsuC2I CCSGG 1 cut(s) 989
AsuHPI GGTGA 2 cut(s) 58, 1442
AsuNHI GCTAGC 1 cut(s) 1034
AvaI CYCGRG 1 cut(s) 738
AvaII GGWCC 1 cut(s) 985
BamHI GGATCC 1 cut(s) 310
BbsI GAAGAC 1 cut(s) 795
BbvI GCAGC 6 cut(s) 23, 253, 398, 1183, 1223, 1552
BccI CCATC 5 cut(s) 197, 1307, 1427, 1522, 1631
BceAI ACGGC 2 cut(s) 148, 548
BcgI CGANNNNNNTGC 2 cut(s) 1625, 1659
BciT130I CCWGG 3 cut(s) 1581, 1610, 1648
BciVI GTATCC 3 cut(s) 689, 1217, 1460
BcnI CCSGG 1 cut(s) 989
BcoDI GTCTC 3 cut(s) 580, 920, 1207
BfaI CTAG 7 cut(s) 327, 347, 1035, 1217, 1341, 1688, 1714
BfmI CTRYAG 2 cut(s) 606, 1626
BfuAI ACCTGC 1 cut(s) 801
BfuI GTATCC 3 cut(s) 689, 1217, 1460
BisI GCNGC 6 cut(s) 12, 267, 387, 1197, 1212, 1566
BlsI GCNGC 6 cut(s) 13, 268, 388, 1198, 1213, 1567
Bme1390I CCNGG 4 cut(s) 989, 1581, 1610, 1648
Bme18I GGWCC 1 cut(s) 985
BmeT110I CYCGRG 1 cut(s) 738
BmgBI CACGTC 1 cut(s) 1615
BmgT120I GGNCC 2 cut(s) 486, 985
BmiI GGNNCC 3 cut(s) 136, 208, 312
BmrFI CCNGG 4 cut(s) 989, 1581, 1610, 1648
BmsI GCATC 6 cut(s) 35, 582, 721, 798, 1051, 1362
BmtI GCTAGC 1 cut(s) 1038
BoxI GACNNNNGTC 1 cut(s) 1638
BpiI GAAGAC 1 cut(s) 795
BpmI CTGGAG 3 cut(s) 282, 427, 1476
BpuMI CCSGG 1 cut(s) 989
BsaBI GATNNNNATC 1 cut(s) 968
BsaJI CCNNGG 4 cut(s) 6, 390, 564, 1077
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 217
Bse1I ACTGG 4 cut(s) 42, 314, 515, 1048
Bse8I GATNNNNATC 1 cut(s) 968
BseBI CCWGG 3 cut(s) 1581, 1610, 1648
BseDI CCNNGG 4 cut(s) 6, 390, 564, 1077
BseGI GGATG 5 cut(s) 208, 969, 1087, 1115, 1270
BseJI GATNNNNATC 1 cut(s) 968
BseLI CCNNNNNNNGG 2 cut(s) 216, 217
BseMII CTCAG 3 cut(s) 446, 575, 1544
BseNI ACTGG 4 cut(s) 42, 314, 515, 1048
BseRI GAGGAG 3 cut(s) 798, 875, 910
BseXI GCAGC 6 cut(s) 23, 253, 398, 1183, 1223, 1552
BseYI CCCAGC 1 cut(s) 1462
BsgI GTGCAG 1 cut(s) 1035
Bsh1236I CGCG 1 cut(s) 1655
BshFI GGCC 4 cut(s) 5, 297, 487, 573
BsiHKCI CYCGRG 1 cut(s) 738
BsiSI CCGG 1 cut(s) 988
BslI CCNNNNNNNGG 2 cut(s) 216, 217
BsmAI GTCTC 3 cut(s) 580, 920, 1207
BsmI GAATGC 2 cut(s) 907, 1626
BsnI GGCC 4 cut(s) 5, 297, 487, 573
BsoBI CYCGRG 1 cut(s) 738
Bsp1407I TGTACA 1 cut(s) 319
Bsp143I GATC 7 cut(s) 244, 310, 410, 864, 1246, 1332, 1710
BspACI CCGC 4 cut(s) 373, 1187, 1386, 1655
BspANI GGCC 4 cut(s) 5, 297, 487, 573
BspCNI CTCAG 3 cut(s) 447, 576, 1545
BspFNI CGCG 1 cut(s) 1655
BspHI TCATGA 4 cut(s) 247, 1143, 1243, 1390
BspLI GGNNCC 3 cut(s) 136, 208, 312
BspMAI CTGCAG 1 cut(s) 610
BspMI ACCTGC 1 cut(s) 801
BspOI GCTAGC 1 cut(s) 1038
BspPI GGATC 5 cut(s) 252, 305, 318, 405, 1241
BsrBI CCGCTC 1 cut(s) 1388
BsrGI TGTACA 1 cut(s) 319
BsrI ACTGG 4 cut(s) 42, 314, 515, 1048
BssECI CCNNGG 4 cut(s) 6, 390, 564, 1077
BssMI GATC 7 cut(s) 244, 310, 410, 864, 1246, 1332, 1710
BssT1I CCWWGG 2 cut(s) 390, 1077
Bst2UI CCWGG 3 cut(s) 1581, 1610, 1648
Bst4CI ACNGT 6 cut(s) 22, 161, 511, 701, 1417, 1630
Bst6I CTCTTC 1 cut(s) 1700
BstAUI TGTACA 1 cut(s) 319
BstC8I GCNNGC 3 cut(s) 571, 1036, 1407
BstDEI CTNAG 4 cut(s) 455, 584, 713, 1553
BstDSI CCRYGG 1 cut(s) 564
BstF5I GGATG 5 cut(s) 208, 969, 1087, 1115, 1270
BstFNI CGCG 1 cut(s) 1655
BstHHI GCGC 2 cut(s) 1211, 1655
BstKTI GATC 7 cut(s) 247, 313, 413, 867, 1249, 1335, 1713
BstMAI GTCTC 3 cut(s) 580, 920, 1207
BstMBI GATC 7 cut(s) 244, 310, 410, 864, 1246, 1332, 1710
BstMWI GCNNNNNNNGC 4 cut(s) 11, 294, 731, 1652
BstNI CCWGG 3 cut(s) 1581, 1610, 1648
BstPAI GACNNNNGTC 1 cut(s) 1638
BstSCI CCNGG 4 cut(s) 987, 1579, 1608, 1646
BstSFI CTRYAG 2 cut(s) 606, 1626
BstUI CGCG 1 cut(s) 1655
BstV1I GCAGC 6 cut(s) 23, 253, 398, 1183, 1223, 1552
BstV2I GAAGAC 1 cut(s) 795
BstX2I RGATCY 3 cut(s) 310, 410, 1710
BstYI RGATCY 3 cut(s) 310, 410, 1710
BsuI GTATCC 3 cut(s) 689, 1217, 1460
BsuRI GGCC 4 cut(s) 5, 297, 487, 573
BtgI CCRYGG 1 cut(s) 564
BtrI CACGTC 1 cut(s) 1615
BtsCI GGATG 5 cut(s) 208, 969, 1087, 1115, 1270
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 4 cut(s) 27, 615, 1055, 1707
BveI ACCTGC 1 cut(s) 801
Cac8I GCNNGC 3 cut(s) 571, 1036, 1407
CciI TCATGA 4 cut(s) 247, 1143, 1243, 1390
CfoI GCGC 2 cut(s) 1211, 1655
Cfr13I GGNCC 2 cut(s) 486, 985
CseI GACGC 1 cut(s) 800
Csp6I GTAC 3 cut(s) 102, 320, 350
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviAII CATG 9 cut(s) 248, 280, 815, 851, 1058, 1144, 1244, 1391, 1643
CviQI GTAC 3 cut(s) 102, 320, 350
DdeI CTNAG 4 cut(s) 455, 584, 713, 1553
DpnI GATC 7 cut(s) 246, 312, 412, 866, 1248, 1334, 1712
DpnII GATC 7 cut(s) 244, 310, 410, 864, 1246, 1332, 1710
EaeI YGGCCR 2 cut(s) 3, 295
Eam1104I CTCTTC 1 cut(s) 1700
EarI CTCTTC 1 cut(s) 1700
EciI GGCGGA 1 cut(s) 362
Eco130I CCWWGG 2 cut(s) 390, 1077
Eco47I GGWCC 1 cut(s) 985
Eco57I CTGAAG 5 cut(s) 198, 444, 747, 782, 1218
Eco88I CYCGRG 1 cut(s) 738
EcoRI GAATTC 1 cut(s) 1485
EcoRII CCWGG 3 cut(s) 1579, 1608, 1646
EcoT14I CCWWGG 2 cut(s) 390, 1077
ErhI CCWWGG 2 cut(s) 390, 1077
FaeI CATG 9 cut(s) 251, 283, 818, 854, 1061, 1147, 1247, 1394, 1646
FalI AAGNNNNNCTT 4 cut(s) 780, 812, 1152, 1184
FatI CATG 9 cut(s) 247, 279, 814, 850, 1057, 1143, 1243, 1390, 1642
Fnu4HI GCNGC 6 cut(s) 12, 267, 387, 1197, 1212, 1566
FokI GGATG 5 cut(s) 215, 976, 1094, 1122, 1277
Fsp4HI GCNGC 6 cut(s) 12, 267, 387, 1197, 1212, 1566
FspBI CTAG 7 cut(s) 327, 347, 1035, 1217, 1341, 1688, 1714
GlaI GCGC 2 cut(s) 1210, 1654
GluI GCNGC 6 cut(s) 12, 267, 387, 1197, 1212, 1566
GsaI CCCAGC 1 cut(s) 1466
GsuI CTGGAG 3 cut(s) 282, 427, 1476
HaeIII GGCC 4 cut(s) 5, 297, 487, 573
HapII CCGG 1 cut(s) 988
HgaI GACGC 1 cut(s) 800
HhaI GCGC 2 cut(s) 1211, 1655
Hin1II CATG 9 cut(s) 251, 283, 818, 854, 1061, 1147, 1247, 1394, 1646
Hin6I GCGC 2 cut(s) 1209, 1653
HinP1I GCGC 2 cut(s) 1209, 1653
HincII GTYRAC 4 cut(s) 304, 657, 949, 1633
HindII GTYRAC 4 cut(s) 304, 657, 949, 1633
HindIII AAGCTT 1 cut(s) 1442
HinfI GANTC 4 cut(s) 531, 546, 835, 1256
HpaII CCGG 1 cut(s) 988
HphI GGTGA 2 cut(s) 58, 1442
Hpy166II GTNNAC 7 cut(s) 304, 322, 657, 949, 985, 1087, 1633
Hpy188I TCNGA 3 cut(s) 31, 751, 801
Hpy8I GTNNAC 7 cut(s) 304, 322, 657, 949, 985, 1087, 1633
HpyAV CCTTC 6 cut(s) 222, 347, 477, 584, 1169, 1421
HpyCH4III ACNGT 6 cut(s) 22, 161, 511, 701, 1417, 1630
HpyCH4IV ACGT 1 cut(s) 1614
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 294, 731, 1652
HpyF3I CTNAG 4 cut(s) 455, 584, 713, 1553
HpySE526I ACGT 1 cut(s) 1614
Hsp92II CATG 9 cut(s) 251, 283, 818, 854, 1061, 1147, 1247, 1394, 1646
HspAI GCGC 2 cut(s) 1209, 1653
Kzo9I GATC 7 cut(s) 244, 310, 410, 864, 1246, 1332, 1710
LmnI GCTCC 2 cut(s) 140, 263
Lsp1109I GCAGC 6 cut(s) 23, 253, 398, 1183, 1223, 1552
LweI GCATC 6 cut(s) 35, 582, 721, 798, 1051, 1362
MaeI CTAG 7 cut(s) 327, 347, 1035, 1217, 1341, 1688, 1714
MaeII ACGT 1 cut(s) 1614
MaeIII GTNAC 4 cut(s) 190, 718, 879, 1282
MalI GATC 7 cut(s) 246, 312, 412, 866, 1248, 1334, 1712
MbiI CCGCTC 1 cut(s) 1388
MboI GATC 7 cut(s) 244, 310, 410, 864, 1246, 1332, 1710
MfeI CAATTG 2 cut(s) 84, 1296
MflI RGATCY 3 cut(s) 310, 410, 1710
MroXI GAANNNNTTC 1 cut(s) 211
MseI TTAA 5 cut(s) 290, 479, 764, 1008, 1574
MspA1I CMGCKG 2 cut(s) 725, 1189
MspI CCGG 1 cut(s) 988
MspR9I CCNGG 4 cut(s) 989, 1581, 1610, 1648
MunI CAATTG 2 cut(s) 84, 1296
Mva1269I GAATGC 2 cut(s) 907, 1626
MvaI CCWGG 3 cut(s) 1581, 1610, 1648
MvnI CGCG 1 cut(s) 1655
MwoI GCNNNNNNNGC 4 cut(s) 11, 294, 731, 1652
NciI CCSGG 1 cut(s) 989
NdeII GATC 7 cut(s) 244, 310, 410, 864, 1246, 1332, 1710
NheI GCTAGC 1 cut(s) 1034
NlaIII CATG 9 cut(s) 251, 283, 818, 854, 1061, 1147, 1247, 1394, 1646
NlaIV GGNNCC 3 cut(s) 136, 208, 312
NmeAIII GCCGAG 2 cut(s) 31, 323
NmuCI GTSAC 1 cut(s) 1282
PaeR7I CTCGAG 1 cut(s) 738
PagI TCATGA 4 cut(s) 247, 1143, 1243, 1390
PctI GAATGC 2 cut(s) 907, 1626
PdmI GAANNNNTTC 1 cut(s) 211
PfeI GAWTC 4 cut(s) 531, 546, 835, 1256
PfoI TCCNGGA 1 cut(s) 1608
PkrI GCNGC 6 cut(s) 13, 268, 388, 1198, 1213, 1567
PshAI GACNNNNGTC 1 cut(s) 1638
Psp6I CCWGG 3 cut(s) 1579, 1608, 1646
PspFI CCCAGC 1 cut(s) 1462
PspGI CCWGG 3 cut(s) 1579, 1608, 1646
PspN4I GGNNCC 3 cut(s) 136, 208, 312
PspPI GGNCC 2 cut(s) 486, 985
PspXI VCTCGAGB 1 cut(s) 738
PstI CTGCAG 1 cut(s) 610
PsuI RGATCY 3 cut(s) 310, 410, 1710
PvuII CAGCTG 1 cut(s) 725
RsaI GTAC 3 cut(s) 103, 321, 351
RsaNI GTAC 3 cut(s) 102, 320, 350
SaqAI TTAA 5 cut(s) 290, 479, 764, 1008, 1574
SatI GCNGC 6 cut(s) 12, 267, 387, 1197, 1212, 1566
Sau3AI GATC 7 cut(s) 244, 310, 410, 864, 1246, 1332, 1710
Sau96I GGNCC 2 cut(s) 486, 985
ScrFI CCNGG 4 cut(s) 989, 1581, 1610, 1648
SfaNI GCATC 6 cut(s) 35, 582, 721, 798, 1051, 1362
SfcI CTRYAG 2 cut(s) 606, 1626
Sfr274I CTCGAG 1 cut(s) 738
SinI GGWCC 1 cut(s) 985
SlaI CTCGAG 1 cut(s) 738
SmlI CTYRAG 1 cut(s) 738
SmoI CTYRAG 1 cut(s) 738
SsiI CCGC 4 cut(s) 373, 1187, 1386, 1655
SspMI CTAG 7 cut(s) 327, 347, 1035, 1217, 1341, 1688, 1714
StyD4I CCNGG 4 cut(s) 987, 1579, 1608, 1646
StyI CCWWGG 2 cut(s) 390, 1077
TaaI ACNGT 6 cut(s) 22, 161, 511, 701, 1417, 1630
TaiI ACGT 1 cut(s) 1617
TaqI TCGA 5 cut(s) 544, 549, 739, 886, 1306
TatI WGTACW 2 cut(s) 319, 349
TfiI GAWTC 4 cut(s) 531, 546, 835, 1256
Tru1I TTAA 5 cut(s) 290, 479, 764, 1008, 1574
Tru9I TTAA 5 cut(s) 290, 479, 764, 1008, 1574
TscAI CASTG 4 cut(s) 27, 615, 1055, 1707
TseFI GTSAC 1 cut(s) 1282
TseI GCWGC 6 cut(s) 11, 266, 386, 1196, 1211, 1565
Tsp45I GTSAC 1 cut(s) 1282
TspDTI ATGAA 9 cut(s) 264, 296, 510, 566, 980, 1104, 1188, 1232, 1440
TspGWI ACGGA 2 cut(s) 257, 323
TspRI CASTG 4 cut(s) 27, 615, 1055, 1707
VpaK11BI GGWCC 1 cut(s) 985
XapI RAATTY 3 cut(s) 744, 995, 1485
XbaI TCTAGA 1 cut(s) 1687
XhoI CTCGAG 1 cut(s) 738
XmnI GAANNNNTTC 1 cut(s) 211
XspI CTAG 7 cut(s) 327, 347, 1035, 1217, 1341, 1688, 1714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.