Rorug06G0211600
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009614
Physical Location & Seq
Forward (+)
33616419 .. 33617942
1524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0211600.1

Sequence Viewer

Length: 1524 bp
ATGACGAACCTTCTTCGTTCAAATCCATGGCGCGGCAATGCCATTTCTAGGGTTTTCGGCGCATTATTCTACTCCACCAATGCCCTAGCTTCCCCACCCTCCTCACCACCCCCACTTCAGTCTCTGCACCGCAGGATTTCCTGGCTCCAAGACCAAGATCTCAAGGCTTCTATTTTCCCAATTCTCAATCACTGGGCTTCGGAAGCTAGAGACGTCAATCAACTGGAGGTCCAATCTTTCATCGTGAAGCTCCGTAAATCCAATCACTTCAACCACGCCCTCCAGGTCTCGACGTGGATGAGCGATGAATTGAAGCTCAATCTGACTCCAGGAGACGTTGCTGCTCGGATAGATTTGATCTCGAGAGTTCACGGCCCCGAAGAAGCCGAGAAGTATTTTGATGGCGTTTCGGAGCAATTGAAAGGTTTGCAAGCTTATTGTGCTCTCTTACATTGCTATGCTCAGCATAGATTGTTGGATAAGGCTGAGAGTGTTTTTGGAAAAATGAAGGAGTTAGGTTTTGAGATAAGAATTAGGTCTTATAATTCAATGTTATGGCTATGTTTTTGGACTGGTAATCATGGAAGGATAGATGTTTTAGTGAAAGAGATGGAAGAAAAAGGCATTGATTATAACCAAGGCACTTTGATGGTTAGGTTGTATTCGTATGCAACCATTTCGGAGATAGGCCGAATGGAGGAGGTTTTGCTGAAGATGGAAGCCCATCCTCTGGTCACTATGAGCTGGCGTAGCTATGTTATTGCAGCCAATGCTTTCTTGAAGGTTGGAGAATTGGAGAAGGTATTGACATTGCTGAAAAGCTCAGAGAAACTCATTAGCAATAGCACAAGGAAAATTGCTTACGAACACCTGCTTTCTTTATATGCTGCTATTGGGAAGAGAGACAAGGTTGATAGAATCTCGAATTTGTATAAAAATTTGGGAAAGTCCTATAGTTATCGTCATATGTTAGTTGCATTGCTGAAAATGGATGACATTGATGGTGCTGAGAAGCTTGTGGAGGAATGTGAATCTGAATCCAAGAAGTTTGACATTCAGATAGGCAACTTGATGGTCAACGCTTACTGCAAGAAAGGTTATTTGGAGAGGGCCAAATCCTTTGTAGAGAAGCTTGCAGAGAGTGGAAAGGAGAATGTAAGCACATGGTGTCTTTTGAGCAAGGGATATTATAGGAATGGGTGTGTGGCGGAGGCATTGGAAGCAATTAAAAAGGCAGCAAACTTGCCATGTCAACCAGGATGGAGGTTCAATCATTCAACTTTGGCTGCATGTCTTGTGTACTTGAAAGAGAACAGAGATGTGGAAACAGCTCATGAATTGCTGAGTTTACTGACAGGAAAGGGTCATATAACGACAATTTTTTGTGAGACCTTAAAAAGTTGTGTTAATGGTGAAACCCAGGTTCTGAGGTCTATAGGGGGTGAACAAGAAAATCATTTGAAGGATAAAATTCAGGACGGAGAGTTGGAGACTATGGATAGTGGAGCCCAACAATGTGGGTAA

Protein Analysis

507

Amino Acids

57.36

Weight (kDa)

7.89

Isoelectric Point (pI)

34.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 145 - 174 2e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 543, 633
AarI CACCTGC 1 cut(s) 879
AatII GACGTC 1 cut(s) 216
Acc36I ACCTGC 1 cut(s) 879
AccB7I CCANNNNNTGG 1 cut(s) 730
AccII CGCG 1 cut(s) 33
AciI CCGC 3 cut(s) 33, 130, 1208
AcsI RAATTY 3 cut(s) 925, 937, 1470
AcuI CTGAAG 2 cut(s) 101, 731
AcyI GRCGYC 1 cut(s) 213
AdeI CACNNNGTG 1 cut(s) 1167
AfaI GTAC 1 cut(s) 1301
AfiI CCNNNNNNNGG 4 cut(s) 32, 48, 697, 730
AjiI CACGTC 1 cut(s) 294
AjnI CCWGG 5 cut(s) 140, 282, 328, 1255, 1419
AjuI GAANNNNNNNTTGG 2 cut(s) 1085, 1117
Alw21I GWGCWC 1 cut(s) 445
Alw26I GTCTC 7 cut(s) 126, 204, 292, 327, 897, 1382, 1484
AlwNI CAGNNNCTG 2 cut(s) 124, 1426
Ama87I CYCGRG 1 cut(s) 361
AoxI GGCC 3 cut(s) 373, 688, 1110
ApeKI GCWGC 5 cut(s) 341, 764, 887, 1235, 1286
ApoI RAATTY 3 cut(s) 925, 937, 1470
AspLEI GCGC 2 cut(s) 33, 62
AspS9I GGNCC 3 cut(s) 229, 374, 1110
AsuHPI GGTGA 3 cut(s) 96, 1424, 1454
AvaI CYCGRG 1 cut(s) 361
AvaII GGWCC 1 cut(s) 229
BanII GRGCYC 1 cut(s) 1510
Bbv12I GWGCWC 1 cut(s) 445
BbvI GCAGC 5 cut(s) 328, 776, 874, 1247, 1273
BccI CCATC 8 cut(s) 395, 604, 643, 709, 732, 995, 1066, 1254
BceAI ACGGC 1 cut(s) 388
BcgI CGANNNNNNTGC 2 cut(s) 660, 694
BciT130I CCWGG 5 cut(s) 142, 284, 330, 1257, 1421
BcoDI GTCTC 7 cut(s) 126, 204, 292, 327, 897, 1382, 1484
BfaI CTAG 3 cut(s) 48, 86, 207
BfmI CTRYAG 2 cut(s) 952, 1434
BfuAI ACCTGC 1 cut(s) 879
BglII AGATCT 1 cut(s) 157
BisI GCNGC 6 cut(s) 34, 342, 765, 888, 1236, 1287
BlpI GCTNAGC 1 cut(s) 462
BlsI GCNGC 6 cut(s) 35, 343, 766, 889, 1237, 1288
Bme1390I CCNGG 5 cut(s) 142, 284, 330, 1257, 1421
Bme18I GGWCC 1 cut(s) 229
BmeT110I CYCGRG 1 cut(s) 361
BmgBI CACGTC 1 cut(s) 294
BmgT120I GGNCC 3 cut(s) 229, 374, 1110
BmiI GGNNCC 3 cut(s) 146, 376, 1507
BmrFI CCNGG 5 cut(s) 142, 284, 330, 1257, 1421
BmrI ACTGGG 1 cut(s) 202
BmuI ACTGGG 1 cut(s) 202
BpmI CTGGAG 3 cut(s) 245, 266, 312
Bpu1102I GCTNAGC 1 cut(s) 462
BpuEI CTTGAG 1 cut(s) 146
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 2 cut(s) 292, 1382
BsaJI CCNNGG 3 cut(s) 26, 637, 1419
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 48, 697, 730
Bse1I ACTGG 3 cut(s) 197, 228, 577
Bse3DI GCAATG 4 cut(s) 43, 451, 809, 977
BseBI CCWGG 5 cut(s) 142, 284, 330, 1257, 1421
BseDI CCNNGG 3 cut(s) 26, 637, 1419
BseGI GGATG 4 cut(s) 303, 724, 997, 1265
BseLI CCNNNNNNNGG 4 cut(s) 32, 48, 697, 730
BseMI GCAATG 4 cut(s) 43, 451, 809, 977
BseMII CTCAG 6 cut(s) 476, 477, 837, 999, 1334, 1418
BseNI ACTGG 3 cut(s) 197, 228, 577
BseRI GAGGAG 2 cut(s) 91, 713
BseXI GCAGC 5 cut(s) 328, 776, 874, 1247, 1273
BsgI GTGCAG 1 cut(s) 110
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 3 cut(s) 375, 690, 1112
BsiHKAI GWGCWC 1 cut(s) 445
BsiHKCI CYCGRG 1 cut(s) 361
BslI CCNNNNNNNGG 4 cut(s) 32, 48, 697, 730
BsmAI GTCTC 7 cut(s) 126, 204, 292, 327, 897, 1382, 1484
BsmBI CGTCTC 2 cut(s) 204, 327
BsnI GGCC 3 cut(s) 375, 690, 1112
Bso31I GGTCTC 2 cut(s) 292, 1382
BsoBI CYCGRG 1 cut(s) 361
Bsp1286I GDGCHC 2 cut(s) 445, 1510
Bsp143I GATC 2 cut(s) 157, 357
Bsp1720I GCTNAGC 1 cut(s) 462
Bsp19I CCATGG 1 cut(s) 26
BspACI CCGC 3 cut(s) 33, 130, 1208
BspANI GGCC 3 cut(s) 375, 690, 1112
BspCNI CTCAG 6 cut(s) 475, 478, 836, 1000, 1335, 1419
BspFNI CGCG 1 cut(s) 33
BspHI TCATGA 1 cut(s) 1333
BspLI GGNNCC 3 cut(s) 146, 376, 1507
BspMI ACCTGC 1 cut(s) 879
BspTNI GGTCTC 2 cut(s) 292, 1382
BsrDI GCAATG 4 cut(s) 43, 451, 809, 977
BsrI ACTGG 3 cut(s) 197, 228, 577
BssECI CCNNGG 3 cut(s) 26, 637, 1419
BssMI GATC 2 cut(s) 157, 357
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 2 cut(s) 26, 637
Bst2UI CCWGG 5 cut(s) 142, 284, 330, 1257, 1421
Bst6I CTCTTC 1 cut(s) 893
BstACI GRCGYC 1 cut(s) 213
BstAPI GCANNNNNTGC 1 cut(s) 770
BstC8I GCNNGC 3 cut(s) 432, 746, 1134
BstDEI CTNAG 6 cut(s) 462, 486, 823, 1008, 1343, 1427
BstDSI CCRYGG 1 cut(s) 26
BstF5I GGATG 4 cut(s) 303, 724, 997, 1265
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 2 cut(s) 33, 62
BstKTI GATC 2 cut(s) 160, 360
BstMAI GTCTC 7 cut(s) 126, 204, 292, 327, 897, 1382, 1484
BstMBI GATC 2 cut(s) 157, 357
BstMWI GCNNNNNNNGC 5 cut(s) 203, 440, 750, 770, 1220
BstNI CCWGG 5 cut(s) 142, 284, 330, 1257, 1421
BstNSI RCATGY 1 cut(s) 1293
BstSCI CCNGG 5 cut(s) 140, 282, 328, 1255, 1419
BstSFI CTRYAG 2 cut(s) 952, 1434
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 5 cut(s) 328, 776, 874, 1247, 1273
BstX2I RGATCY 1 cut(s) 157
BstXI CCANNNNNNTGG 1 cut(s) 1517
BstYI RGATCY 1 cut(s) 157
BsuRI GGCC 3 cut(s) 375, 690, 1112
BtgI CCRYGG 1 cut(s) 26
BtgZI GCGATG 1 cut(s) 318
BtrI CACGTC 1 cut(s) 294
BtsCI GGATG 4 cut(s) 303, 724, 997, 1265
BtsIMutI CAGTG 1 cut(s) 190
BveI ACCTGC 1 cut(s) 879
Cac8I GCNNGC 3 cut(s) 432, 746, 1134
CaiI CAGNNNCTG 2 cut(s) 124, 1426
CciI TCATGA 1 cut(s) 1333
CfoI GCGC 2 cut(s) 33, 62
Cfr13I GGNCC 3 cut(s) 229, 374, 1110
Csp6I GTAC 1 cut(s) 1300
CviAII CATG 6 cut(s) 27, 581, 1164, 1248, 1290, 1334
CviQI GTAC 1 cut(s) 1300
DdeI CTNAG 6 cut(s) 462, 486, 823, 1008, 1343, 1427
DpnI GATC 2 cut(s) 159, 359
DpnII GATC 2 cut(s) 157, 357
DraIII CACNNNGTG 1 cut(s) 1167
Eam1104I CTCTTC 1 cut(s) 893
EarI CTCTTC 1 cut(s) 893
EciI GGCGGA 1 cut(s) 1223
Eco130I CCWWGG 2 cut(s) 26, 637
Eco24I GRGCYC 1 cut(s) 1510
Eco31I GGTCTC 2 cut(s) 292, 1382
Eco47I GGWCC 1 cut(s) 229
Eco57I CTGAAG 2 cut(s) 101, 731
Eco88I CYCGRG 1 cut(s) 361
EcoRII CCWGG 5 cut(s) 140, 282, 328, 1255, 1419
EcoT14I CCWWGG 2 cut(s) 26, 637
EcoT38I GRGCYC 1 cut(s) 1510
ErhI CCWWGG 2 cut(s) 26, 637
Esp3I CGTCTC 2 cut(s) 204, 327
FaeI CATG 6 cut(s) 30, 584, 1167, 1251, 1293, 1337
FatI CATG 6 cut(s) 26, 580, 1163, 1247, 1289, 1333
FauNDI CATATG 1 cut(s) 966
Fnu4HI GCNGC 6 cut(s) 34, 342, 765, 888, 1236, 1287
FokI GGATG 4 cut(s) 310, 711, 1004, 1272
FriOI GRGCYC 1 cut(s) 1510
Fsp4HI GCNGC 6 cut(s) 34, 342, 765, 888, 1236, 1287
FspBI CTAG 3 cut(s) 48, 86, 207
GlaI GCGC 2 cut(s) 32, 61
GluI GCNGC 6 cut(s) 34, 342, 765, 888, 1236, 1287
GsuI CTGGAG 3 cut(s) 245, 266, 312
HaeIII GGCC 3 cut(s) 375, 690, 1112
HhaI GCGC 2 cut(s) 33, 62
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 6 cut(s) 30, 584, 1167, 1251, 1293, 1337
Hin6I GCGC 2 cut(s) 31, 60
HinP1I GCGC 2 cut(s) 31, 60
HincII GTYRAC 2 cut(s) 1078, 1253
HindII GTYRAC 2 cut(s) 1078, 1253
HindIII AAGCTT 3 cut(s) 432, 1013, 1130
HinfI GANTC 4 cut(s) 325, 918, 1031, 1037
HphI GGTGA 3 cut(s) 96, 1424, 1454
Hpy166II GTNNAC 6 cut(s) 370, 1078, 1253, 1300, 1349, 1445
Hpy188I TCNGA 9 cut(s) 202, 324, 348, 412, 682, 826, 1036, 1059, 1428
Hpy188III TCNNGA 8 cut(s) 244, 289, 361, 363, 778, 922, 1334, 1475
Hpy8I GTNNAC 6 cut(s) 370, 1078, 1253, 1300, 1349, 1445
Hpy99I CGWCG 1 cut(s) 295
HpyAV CCTTC 6 cut(s) 20, 502, 579, 775, 793, 1456
HpyCH4IV ACGT 3 cut(s) 213, 293, 336
HpyCH4V TGCA 8 cut(s) 127, 430, 671, 764, 977, 1089, 1136, 1289
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 440, 750, 770, 1220
HpyF3I CTNAG 6 cut(s) 462, 486, 823, 1008, 1343, 1427
HpySE526I ACGT 3 cut(s) 213, 293, 336
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 6 cut(s) 30, 584, 1167, 1251, 1293, 1337
HspAI GCGC 2 cut(s) 31, 60
Kzo9I GATC 2 cut(s) 157, 357
LmnI GCTCC 4 cut(s) 150, 255, 412, 1505
Lsp1109I GCAGC 5 cut(s) 328, 776, 874, 1247, 1273
MaeI CTAG 3 cut(s) 48, 86, 207
MaeII ACGT 3 cut(s) 213, 293, 336
MaeIII GTNAC 1 cut(s) 733
MalI GATC 2 cut(s) 159, 359
MboI GATC 2 cut(s) 157, 357
MboII GAAGA 5 cut(s) 5, 392, 626, 724, 910
MfeI CAATTG 1 cut(s) 416
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 2 cut(s) 445, 1510
MlyI GAGTC 1 cut(s) 319
MmeI TCCRAC 3 cut(s) 456, 766, 1467
MseI TTAA 3 cut(s) 1227, 1394, 1407
MslI CAYNNNNRTG 2 cut(s) 456, 647
MspR9I CCNGG 5 cut(s) 142, 284, 330, 1257, 1421
MunI CAATTG 1 cut(s) 416
MvaI CCWGG 5 cut(s) 142, 284, 330, 1257, 1421
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 5 cut(s) 203, 440, 750, 770, 1220
NcoI CCATGG 1 cut(s) 26
NdeI CATATG 1 cut(s) 966
NdeII GATC 2 cut(s) 157, 357
NlaIII CATG 6 cut(s) 30, 584, 1167, 1251, 1293, 1337
NlaIV GGNNCC 3 cut(s) 146, 376, 1507
NmeAIII GCCGAG 1 cut(s) 412
NmuCI GTSAC 1 cut(s) 733
NspI RCATGY 1 cut(s) 1293
PaeR7I CTCGAG 1 cut(s) 361
PagI TCATGA 1 cut(s) 1333
PaqCI CACCTGC 1 cut(s) 879
PfeI GAWTC 3 cut(s) 918, 1031, 1037
PflMI CCANNNNNTGG 1 cut(s) 730
PfoI TCCNGGA 1 cut(s) 328
PkrI GCNGC 6 cut(s) 35, 343, 766, 889, 1237, 1288
PleI GAGTC 1 cut(s) 319
PpsI GAGTC 1 cut(s) 319
PsiI TTATAA 2 cut(s) 543, 633
Psp6I CCWGG 5 cut(s) 140, 282, 328, 1255, 1419
PspGI CCWGG 5 cut(s) 140, 282, 328, 1255, 1419
PspN4I GGNNCC 3 cut(s) 146, 376, 1507
PspPI GGNCC 3 cut(s) 229, 374, 1110
PstNI CAGNNNCTG 2 cut(s) 124, 1426
PsuI RGATCY 1 cut(s) 157
RsaI GTAC 1 cut(s) 1301
RsaNI GTAC 1 cut(s) 1300
RseI CAYNNNNRTG 2 cut(s) 456, 647
SaqAI TTAA 3 cut(s) 1227, 1394, 1407
SatI GCNGC 6 cut(s) 34, 342, 765, 888, 1236, 1287
Sau3AI GATC 2 cut(s) 157, 357
Sau96I GGNCC 3 cut(s) 229, 374, 1110
SchI GAGTC 1 cut(s) 319
ScrFI CCNGG 5 cut(s) 142, 284, 330, 1257, 1421
SduI GDGCHC 2 cut(s) 445, 1510
SfcI CTRYAG 2 cut(s) 952, 1434
Sfr274I CTCGAG 1 cut(s) 361
SinI GGWCC 1 cut(s) 229
SlaI CTCGAG 1 cut(s) 361
SmiMI CAYNNNNRTG 2 cut(s) 456, 647
SmlI CTYRAG 2 cut(s) 161, 361
SmoI CTYRAG 2 cut(s) 161, 361
SsiI CCGC 3 cut(s) 33, 130, 1208
SspMI CTAG 3 cut(s) 48, 86, 207
StyD4I CCNGG 5 cut(s) 140, 282, 328, 1255, 1419
StyI CCWWGG 2 cut(s) 26, 637
TaiI ACGT 3 cut(s) 216, 296, 339
TaqI TCGA 3 cut(s) 290, 362, 923
TatI WGTACW 1 cut(s) 1299
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 3 cut(s) 918, 1031, 1037
Tru1I TTAA 3 cut(s) 1227, 1394, 1407
Tru9I TTAA 3 cut(s) 1227, 1394, 1407
TscAI CASTG 1 cut(s) 197
TseFI GTSAC 1 cut(s) 733
TseI GCWGC 5 cut(s) 341, 764, 887, 1235, 1286
Tsp45I GTSAC 1 cut(s) 733
TspDTI ATGAA 4 cut(s) 229, 321, 521, 1350
TspGWI ACGGA 2 cut(s) 242, 1494
TspRI CASTG 1 cut(s) 197
Van91I CCANNNNNTGG 1 cut(s) 730
VpaK11BI GGWCC 1 cut(s) 229
XapI RAATTY 3 cut(s) 925, 937, 1470
XceI RCATGY 1 cut(s) 1293
XhoI CTCGAG 1 cut(s) 361
XspI CTAG 3 cut(s) 48, 86, 207
ZraI GACGTC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.