Rroxscaffold_5G00360230
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009614
Physical Location & Seq
Reverse (-)
40587048 .. 40590304
3257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00360230.1

Sequence Viewer

Length: 1716 bp
ATGGCTGAGGCAGCATACACAGTGGCATCTGATAGTGAAACCAGTCGGGAGGAGAAATCCTCATGTACCTTTCCTGAAACAACAATTGGAATTGATATTGGCACTTCACAATGCAGGGTTGCGATCTGGAATGGCTCCCAGGTTGAGATCCTTAAAAACAGTAGGAACCAGAAGATGATGAGTTCATATGTTACATTCAAGGATGGAACTCCTTCTGGGGAACTAAGCAATCAACTCTCTGCAGATCATGATATGTTATCCGGAGCTGCAATCTTCAACATGAAACGCTTAATTGGCCGAGTTGACACGGATCCAGTTGTCCACTCTAGCAAAAGCCTTCCATTTCTAGTACAAACATTGGACATTGGTGTCCGCCCATTTATTGCAGCCTTGGTTGGAAATTTCTGGAGATCCACCACTCCTGAAGAAGTTCTGGCAATATTTTTGGTGGAACTTAGAGAAATGGCAGAAATACAGTTAAAGCGGCCCATAAAAAATGTTGTTCTGACTCTGCCAGTTTCATTCAGCCGATTCCAGCTGACTCGGATTGAGAGAGCATGTGCCATGGCTGGCCTTCATATCCTGAAACTGATGCCAGAGCCAACTGCAGTGGCATTGTTATATACCTTATATGCAAAACAAAGTGGATTTCAGTCAACAGATAGGAATGTTCTAATCTTCAATATGGGTGCTGGATACTGTGATGTCGCCGTAAGTGTTACAGCTGAAGAGGCACCTCGAGGAATTTCCGAAATAATTGCCTTAACAGGAAGTGCCATTGGAGGAGAAGACTTACTTCAGAATATGATGCGTCATCTGTTGCCAAATGCAGAGAATCTTTTGACAAGTCATGGAGTTGATGAAATCAAATCAAAGGGGTTACTACGAGTTGCGACCCAGAATGCAATCCACAGTCTCTCCTCCCAAAATTCTGTTCAAATTGATGTTGACTTGGGAAATGGGATGAAAATCTGCAAGGTTGTGGACCGAGAAGAATTTGAGGAAGTTAATCGGGCCATATTTGATAAGTGTGCTAGCCTCATAATCCAGTGTTTGCATGATGCAAAGGTAAAACCTGAGGATGTGAACGATGTAGTACTTGTAGGTGGATGTTCATACATTCCAAAGATAAAAAATCTTGTCATGAGCGTATGCAAGAAAGAGGCGCTTTACGAAGGGATGAATCCATTGGAAGCTGCTGTCTCTGGAGCAGCACTACAAGGATACGTAGCTTCAGGTATTCATGATCCTTTTGAATCCGTGGATGTGCTATCTATTCAGGTCACACCTCTCTCAATTGGAATTCGAGCTGACGGAAACAACTTTGTGCCTATCATACCTCGAAACACCACAATACCAGCCCAAAGAGAGATGCTTTTCACAACATCCCATGATAATCAAGCCGAAGCACTTGTGATTGTTTATGAAGGTGATGGCGAGAAGCTGGAAGAAAATCATTTGCTGGGATACTTCAAAATTGAGGGAATTCCTCCAGCACCCAAAGGAATTCCACAAGTACGAGTAATGATGGACATTGATGCTTCAAACGTGCTGAGAGTTTTGGCTGCTGTATTAATGCCAGGTTCTCATGTTCCCATAAATCCTGTCCTGGACGTGAGGATGCCGACGGTTGACGATGGTCATGGCTGGTGCACGGAAGCGTTGCACAGAGCTTATGGTGCTACTCAAGACTTAGTATCAGTGAAGAGGATCTAG

Protein Analysis

571

Amino Acids

62.23

Weight (kDa)

5.36

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 29 - 528 1.6e-96 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
AbsI CCTCGAGG 1 cut(s) 738
AccB1I GGYRCC 1 cut(s) 733
AccIII TCCGGA 1 cut(s) 260
AciI CCGC 2 cut(s) 373, 484
AclWI GGATC 5 cut(s) 142, 305, 318, 405, 1241
AcoI YGGCCR 1 cut(s) 295
AcsI RAATTY 7 cut(s) 400, 744, 928, 995, 1302, 1485, 1506
AcuI CTGAAG 4 cut(s) 444, 747, 782, 1218
AfaI GTAC 4 cut(s) 67, 351, 1098, 1518
AgsI TTSAA 7 cut(s) 199, 277, 682, 938, 1256, 1474, 1545
AjiI CACGTC 1 cut(s) 1615
AjnI CCWGG 3 cut(s) 138, 1579, 1608
AjuI GAANNNNNNNTTGG 4 cut(s) 69, 101, 918, 950
AluBI AGCT 8 cut(s) 266, 538, 725, 1196, 1232, 1310, 1444, 1673
AluI AGCT 8 cut(s) 266, 538, 725, 1196, 1232, 1310, 1444, 1673
Alw21I GWGCWC 1 cut(s) 1655
Alw26I GTCTC 2 cut(s) 920, 1207
Alw44I GTGCAC 1 cut(s) 1651
AlwI GGATC 5 cut(s) 142, 305, 318, 405, 1241
Ama87I CYCGRG 1 cut(s) 738
Aor13HI TCCGGA 1 cut(s) 260
AoxI GGCC 4 cut(s) 295, 485, 571, 1014
ApaLI GTGCAC 1 cut(s) 1651
ApeKI GCWGC 6 cut(s) 11, 266, 386, 1196, 1211, 1565
ApoI RAATTY 7 cut(s) 400, 744, 928, 995, 1302, 1485, 1506
AseI ATTAAT 1 cut(s) 1574
Asp700I GAANNNNTTC 2 cut(s) 211, 429
AspLEI GCGC 1 cut(s) 1168
AspS9I GGNCC 3 cut(s) 486, 985, 1014
AsuHPI GGTGA 1 cut(s) 1442
AsuNHI GCTAGC 1 cut(s) 1034
AvaI CYCGRG 1 cut(s) 738
AvaII GGWCC 1 cut(s) 985
AxyI CCTNAGG 1 cut(s) 1077
BaeGI GKGCMC 1 cut(s) 1655
BamHI GGATCC 1 cut(s) 310
BanI GGYRCC 1 cut(s) 733
BbsI GAAGAC 1 cut(s) 795
Bbv12I GWGCWC 1 cut(s) 1655
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 6 cut(s) 23, 253, 398, 1183, 1223, 1552
BccI CCATC 4 cut(s) 197, 1427, 1522, 1631
BceAI ACGGC 1 cut(s) 695
BcgI CGANNNNNNTGC 2 cut(s) 740, 774
BciT130I CCWGG 3 cut(s) 140, 1581, 1610
BciVI GTATCC 3 cut(s) 689, 1217, 1460
BcoDI GTCTC 2 cut(s) 920, 1207
BfaI CTAG 4 cut(s) 327, 347, 1035, 1714
BfmI CTRYAG 2 cut(s) 240, 606
BfoI RGCGCY 1 cut(s) 1169
BfuI GTATCC 3 cut(s) 689, 1217, 1460
BisI GCNGC 7 cut(s) 12, 267, 387, 485, 1197, 1212, 1566
BlsI GCNGC 7 cut(s) 13, 268, 388, 486, 1198, 1213, 1567
BmcAI AGTACT 1 cut(s) 1098
Bme1390I CCNGG 3 cut(s) 140, 1581, 1610
Bme18I GGWCC 1 cut(s) 985
BmeT110I CYCGRG 1 cut(s) 738
BmgBI CACGTC 1 cut(s) 1615
BmgT120I GGNCC 3 cut(s) 486, 985, 1014
BmiI GGNNCC 4 cut(s) 136, 167, 312, 735
BmrFI CCNGG 3 cut(s) 140, 1581, 1610
BmsI GCATC 7 cut(s) 35, 582, 798, 1051, 1362, 1528, 1611
BmtI GCTAGC 1 cut(s) 1038
BoxI GACNNNNGTC 1 cut(s) 1638
BpiI GAAGAC 1 cut(s) 795
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpmI CTGGAG 3 cut(s) 427, 1227, 1476
Bpu10I CCTNAGC 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 1671
BsaAI YACGTR 1 cut(s) 1228
BsaBI GATNNNNATC 1 cut(s) 968
BsaJI CCNNGG 4 cut(s) 138, 390, 564, 1260
BsaWI WCCGGW 1 cut(s) 260
BsaXI ACNNNNNCTCC 2 cut(s) 902, 932
Bse1I ACTGG 4 cut(s) 42, 314, 515, 1048
Bse21I CCTNAGG 1 cut(s) 1077
Bse8I GATNNNNATC 1 cut(s) 968
BseAI TCCGGA 1 cut(s) 260
BseBI CCWGG 3 cut(s) 140, 1581, 1610
BseDI CCNNGG 4 cut(s) 138, 390, 564, 1260
BseGI GGATG 8 cut(s) 208, 969, 1087, 1115, 1185, 1270, 1385, 1626
BseJI GATNNNNATC 1 cut(s) 968
BseMII CTCAG 2 cut(s) 1068, 1544
BseNI ACTGG 4 cut(s) 42, 314, 515, 1048
BseRI GAGGAG 3 cut(s) 65, 798, 910
BseSI GKGCMC 1 cut(s) 1655
BseXI GCAGC 6 cut(s) 23, 253, 398, 1183, 1223, 1552
BseYI CCCAGC 1 cut(s) 1462
BshFI GGCC 4 cut(s) 297, 487, 573, 1016
BshNI GGYRCC 1 cut(s) 733
BsiHKAI GWGCWC 1 cut(s) 1655
BsiHKCI CYCGRG 1 cut(s) 738
BsiSI CCGG 1 cut(s) 261
BsmAI GTCTC 2 cut(s) 920, 1207
BsmI GAATGC 1 cut(s) 907
BsnI GGCC 4 cut(s) 297, 487, 573, 1016
BsoBI CYCGRG 1 cut(s) 738
Bsp1286I GDGCHC 1 cut(s) 1655
Bsp13I TCCGGA 1 cut(s) 260
Bsp143I GATC 7 cut(s) 123, 147, 244, 310, 410, 1246, 1710
Bsp19I CCATGG 1 cut(s) 564
BspACI CCGC 2 cut(s) 373, 484
BspANI GGCC 4 cut(s) 297, 487, 573, 1016
BspCNI CTCAG 2 cut(s) 1069, 1545
BspEI TCCGGA 1 cut(s) 260
BspHI TCATGA 3 cut(s) 247, 1143, 1243
BspLI GGNNCC 4 cut(s) 136, 167, 312, 735
BspMAI CTGCAG 2 cut(s) 244, 610
BspOI GCTAGC 1 cut(s) 1038
BspPI GGATC 5 cut(s) 142, 305, 318, 405, 1241
BspT107I GGYRCC 1 cut(s) 733
BsrI ACTGG 4 cut(s) 42, 314, 515, 1048
BssECI CCNNGG 4 cut(s) 138, 390, 564, 1260
BssMI GATC 7 cut(s) 123, 147, 244, 310, 410, 1246, 1710
BssT1I CCWWGG 2 cut(s) 390, 564
Bst2UI CCWGG 3 cut(s) 140, 1581, 1610
Bst4CI ACNGT 6 cut(s) 22, 161, 477, 701, 914, 1630
Bst6I CTCTTC 2 cut(s) 723, 1700
BstBAI YACGTR 1 cut(s) 1228
BstC8I GCNNGC 2 cut(s) 571, 1036
BstDEI CTNAG 6 cut(s) 6, 224, 455, 1077, 1553, 1693
BstDSI CCRYGG 2 cut(s) 564, 1260
BstF5I GGATG 8 cut(s) 208, 969, 1087, 1115, 1185, 1270, 1385, 1626
BstH2I RGCGCY 1 cut(s) 1169
BstHHI GCGC 1 cut(s) 1168
BstKTI GATC 7 cut(s) 126, 150, 247, 313, 413, 1249, 1713
BstMAI GTCTC 2 cut(s) 920, 1207
BstMBI GATC 7 cut(s) 123, 147, 244, 310, 410, 1246, 1710
BstMWI GCNNNNNNNGC 4 cut(s) 11, 294, 731, 1679
BstNI CCWGG 3 cut(s) 140, 1581, 1610
BstNSI RCATGY 1 cut(s) 561
BstPAI GACNNNNGTC 1 cut(s) 1638
BstSCI CCNGG 3 cut(s) 138, 1579, 1608
BstSFI CTRYAG 2 cut(s) 240, 606
BstSLI GKGCMC 1 cut(s) 1655
BstSNI TACGTA 1 cut(s) 1228
BstV1I GCAGC 6 cut(s) 23, 253, 398, 1183, 1223, 1552
BstV2I GAAGAC 1 cut(s) 795
BstX2I RGATCY 4 cut(s) 147, 310, 410, 1710
BstYI RGATCY 4 cut(s) 147, 310, 410, 1710
Bsu36I CCTNAGG 1 cut(s) 1077
BsuI GTATCC 3 cut(s) 689, 1217, 1460
BsuRI GGCC 4 cut(s) 297, 487, 573, 1016
BtgI CCRYGG 2 cut(s) 564, 1260
BtrI CACGTC 1 cut(s) 1615
BtsCI GGATG 8 cut(s) 208, 969, 1087, 1115, 1185, 1270, 1385, 1626
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 4 cut(s) 27, 615, 1055, 1707
Cac8I GCNNGC 2 cut(s) 571, 1036
CciI TCATGA 3 cut(s) 247, 1143, 1243
CfoI GCGC 1 cut(s) 1168
Cfr13I GGNCC 3 cut(s) 486, 985, 1014
CseI GACGC 1 cut(s) 800
Csp6I GTAC 4 cut(s) 66, 350, 1097, 1517
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviQI GTAC 4 cut(s) 66, 350, 1097, 1517
DdeI CTNAG 6 cut(s) 6, 224, 455, 1077, 1553, 1693
DpnI GATC 7 cut(s) 125, 149, 246, 312, 412, 1248, 1712
DpnII GATC 7 cut(s) 123, 147, 244, 310, 410, 1246, 1710
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 1 cut(s) 295
Eam1104I CTCTTC 2 cut(s) 723, 1700
EarI CTCTTC 2 cut(s) 723, 1700
EciI GGCGGA 1 cut(s) 362
Eco105I TACGTA 1 cut(s) 1228
Eco130I CCWWGG 2 cut(s) 390, 564
Eco47I GGWCC 1 cut(s) 985
Eco57I CTGAAG 4 cut(s) 444, 747, 782, 1218
Eco81I CCTNAGG 1 cut(s) 1077
Eco88I CYCGRG 1 cut(s) 738
EcoRI GAATTC 3 cut(s) 1302, 1485, 1506
EcoRII CCWGG 3 cut(s) 138, 1579, 1608
EcoT14I CCWWGG 2 cut(s) 390, 564
ErhI CCWWGG 2 cut(s) 390, 564
FalI AAGNNNNNCTT 4 cut(s) 780, 812, 1152, 1184
FauNDI CATATG 1 cut(s) 187
Fnu4HI GCNGC 7 cut(s) 12, 267, 387, 485, 1197, 1212, 1566
FokI GGATG 8 cut(s) 215, 976, 1094, 1122, 1192, 1277, 1372, 1633
Fsp4HI GCNGC 7 cut(s) 12, 267, 387, 485, 1197, 1212, 1566
FspBI CTAG 4 cut(s) 327, 347, 1035, 1714
GlaI GCGC 1 cut(s) 1167
GluI GCNGC 7 cut(s) 12, 267, 387, 485, 1197, 1212, 1566
GsaI CCCAGC 1 cut(s) 1466
GsuI CTGGAG 3 cut(s) 427, 1227, 1476
HaeII RGCGCY 1 cut(s) 1169
HaeIII GGCC 4 cut(s) 297, 487, 573, 1016
HapII CCGG 1 cut(s) 261
HgaI GACGC 1 cut(s) 800
HhaI GCGC 1 cut(s) 1168
Hin6I GCGC 1 cut(s) 1166
HinP1I GCGC 1 cut(s) 1166
HincII GTYRAC 4 cut(s) 304, 657, 949, 1633
HindII GTYRAC 4 cut(s) 304, 657, 949, 1633
HinfI GANTC 6 cut(s) 508, 531, 541, 835, 1183, 1256
HpaII CCGG 1 cut(s) 261
HphI GGTGA 1 cut(s) 1442
Hpy166II GTNNAC 8 cut(s) 304, 322, 657, 949, 985, 1087, 1633, 1653
Hpy188I TCNGA 5 cut(s) 31, 507, 546, 751, 801
Hpy8I GTNNAC 8 cut(s) 304, 322, 657, 949, 985, 1087, 1633, 1653
Hpy99I CGWCG 1 cut(s) 1630
HpyAV CCTTC 5 cut(s) 222, 347, 584, 1169, 1421
HpyCH4III ACNGT 6 cut(s) 22, 161, 477, 701, 914, 1630
HpyCH4IV ACGT 3 cut(s) 1227, 1548, 1614
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 294, 731, 1679
HpyF3I CTNAG 6 cut(s) 6, 224, 455, 1077, 1553, 1693
HpySE526I ACGT 3 cut(s) 1227, 1548, 1614
HspAI GCGC 1 cut(s) 1166
Kpn2I TCCGGA 1 cut(s) 260
Kzo9I GATC 7 cut(s) 123, 147, 244, 310, 410, 1246, 1710
LmnI GCTCC 3 cut(s) 140, 263, 1208
Lsp1109I GCAGC 6 cut(s) 23, 253, 398, 1183, 1223, 1552
LweI GCATC 7 cut(s) 35, 582, 798, 1051, 1362, 1528, 1611
MaeI CTAG 4 cut(s) 327, 347, 1035, 1714
MaeII ACGT 3 cut(s) 1227, 1548, 1614
MaeIII GTNAC 4 cut(s) 190, 718, 879, 1282
MalI GATC 7 cut(s) 125, 149, 246, 312, 412, 1248, 1712
MboI GATC 7 cut(s) 123, 147, 244, 310, 410, 1246, 1710
MboII GAAGA 8 cut(s) 184, 265, 437, 670, 740, 800, 1004, 1460
MfeI CAATTG 2 cut(s) 84, 1296
MflI RGATCY 4 cut(s) 147, 310, 410, 1710
MhlI GDGCHC 1 cut(s) 1655
MlyI GAGTC 2 cut(s) 502, 535
MmeI TCCRAC 1 cut(s) 376
MroI TCCGGA 1 cut(s) 260
MroXI GAANNNNTTC 2 cut(s) 211, 429
MseI TTAA 6 cut(s) 153, 290, 479, 764, 1008, 1574
MspA1I CMGCKG 2 cut(s) 538, 725
MspI CCGG 1 cut(s) 261
MspR9I CCNGG 3 cut(s) 140, 1581, 1610
MunI CAATTG 2 cut(s) 84, 1296
Mva1269I GAATGC 1 cut(s) 907
MvaI CCWGG 3 cut(s) 140, 1581, 1610
MwoI GCNNNNNNNGC 4 cut(s) 11, 294, 731, 1679
NcoI CCATGG 1 cut(s) 564
NdeI CATATG 1 cut(s) 187
NdeII GATC 7 cut(s) 123, 147, 244, 310, 410, 1246, 1710
NheI GCTAGC 1 cut(s) 1034
NlaIV GGNNCC 4 cut(s) 136, 167, 312, 735
NmeAIII GCCGAG 1 cut(s) 323
NmuCI GTSAC 1 cut(s) 1282
NspI RCATGY 1 cut(s) 561
PaeR7I CTCGAG 1 cut(s) 738
PagI TCATGA 3 cut(s) 247, 1143, 1243
PctI GAATGC 1 cut(s) 907
PdmI GAANNNNTTC 2 cut(s) 211, 429
PfeI GAWTC 4 cut(s) 531, 835, 1183, 1256
PfoI TCCNGGA 1 cut(s) 1608
PkrI GCNGC 7 cut(s) 13, 268, 388, 486, 1198, 1213, 1567
PleI GAGTC 2 cut(s) 502, 535
PpsI GAGTC 2 cut(s) 502, 535
Ppu21I YACGTR 1 cut(s) 1228
PshAI GACNNNNGTC 1 cut(s) 1638
PshBI ATTAAT 1 cut(s) 1574
Psp6I CCWGG 3 cut(s) 138, 1579, 1608
PspFI CCCAGC 1 cut(s) 1462
PspGI CCWGG 3 cut(s) 138, 1579, 1608
PspN4I GGNNCC 4 cut(s) 136, 167, 312, 735
PspPI GGNCC 3 cut(s) 486, 985, 1014
PspXI VCTCGAGB 1 cut(s) 738
PstI CTGCAG 2 cut(s) 244, 610
PsuI RGATCY 4 cut(s) 147, 310, 410, 1710
PvuII CAGCTG 2 cut(s) 538, 725
RsaI GTAC 4 cut(s) 67, 351, 1098, 1518
RsaNI GTAC 4 cut(s) 66, 350, 1097, 1517
SaqAI TTAA 6 cut(s) 153, 290, 479, 764, 1008, 1574
SatI GCNGC 7 cut(s) 12, 267, 387, 485, 1197, 1212, 1566
Sau3AI GATC 7 cut(s) 123, 147, 244, 310, 410, 1246, 1710
Sau96I GGNCC 3 cut(s) 486, 985, 1014
ScaI AGTACT 1 cut(s) 1098
SchI GAGTC 2 cut(s) 502, 535
ScrFI CCNGG 3 cut(s) 140, 1581, 1610
SduI GDGCHC 1 cut(s) 1655
SfaNI GCATC 7 cut(s) 35, 582, 798, 1051, 1362, 1528, 1611
SfcI CTRYAG 2 cut(s) 240, 606
Sfr274I CTCGAG 1 cut(s) 738
SinI GGWCC 1 cut(s) 985
SlaI CTCGAG 1 cut(s) 738
SmlI CTYRAG 2 cut(s) 738, 1686
SmoI CTYRAG 2 cut(s) 738, 1686
SnaBI TACGTA 1 cut(s) 1228
SsiI CCGC 2 cut(s) 373, 484
SspI AATATT 1 cut(s) 441
SspMI CTAG 4 cut(s) 327, 347, 1035, 1714
StyD4I CCNGG 3 cut(s) 138, 1579, 1608
StyI CCWWGG 2 cut(s) 390, 564
TaaI ACNGT 6 cut(s) 22, 161, 477, 701, 914, 1630
TaiI ACGT 3 cut(s) 1230, 1551, 1617
TaqI TCGA 3 cut(s) 739, 1306, 1342
TaqII GACCGA 1 cut(s) 1002
TatI WGTACW 2 cut(s) 349, 1096
TauI GCSGC 1 cut(s) 487
TfiI GAWTC 4 cut(s) 531, 835, 1183, 1256
Tru1I TTAA 6 cut(s) 153, 290, 479, 764, 1008, 1574
Tru9I TTAA 6 cut(s) 153, 290, 479, 764, 1008, 1574
TscAI CASTG 4 cut(s) 27, 615, 1055, 1707
TseFI GTSAC 1 cut(s) 1282
TseI GCWGC 6 cut(s) 11, 266, 386, 1196, 1211, 1565
Tsp45I GTSAC 1 cut(s) 1282
TspGWI ACGGA 4 cut(s) 323, 1249, 1329, 1670
TspRI CASTG 4 cut(s) 27, 615, 1055, 1707
VneI GTGCAC 1 cut(s) 1651
VpaK11BI GGWCC 1 cut(s) 985
VspI ATTAAT 1 cut(s) 1574
XapI RAATTY 7 cut(s) 400, 744, 928, 995, 1302, 1485, 1506
XceI RCATGY 1 cut(s) 561
XhoI CTCGAG 1 cut(s) 738
XmnI GAANNNNTTC 2 cut(s) 211, 429
XspI CTAG 4 cut(s) 327, 347, 1035, 1714
ZrmI AGTACT 1 cut(s) 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.