pycom16g16230
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009614
Physical Location & Seq
Forward (+)
12172743 .. 12174278
1536 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16230.1

Sequence Viewer

Length: 1536 bp
ATGATGCGGTCGTATGTCACATTCAAGGATGAAAACCCTTCAGCGGGAGTAAGCGATCAACTCTCCAGTGAGCACGACATGTTATCTGGAGCTTCAATCTTCAACATGAAACGATTAATTGGCAGGGTTGACACCGATCCAGTTGTTCAGTCAAGCAAAAGCCTCCCCTTTTTGGTTCAAACATTGGGCATTGGGGTGCGTCCACTGATCGCAGCCTTGGTGAACAATGTATGGAGATCCACCACTCCTGAAGAAGTTCTGGCAATATTTTTTGTGGAACTCAAGGCAATGGCAGAAATCCAGCTAAAGCGGCCCATAAAGAATGTTGTTCTCACCATTCCAGCTTCATTCAGCCGATTCCAGCTGACTCGTATTGAAAGAGCTTGTGCCATGGCTAGCCTTCATGTACTCAGACTGATGCCTGAACCAACTGCTATCGCGCTGTTATACGCGCAACAGATTTCAAATCAGAACGTAAACACCGGATCTGAGAAGATTGCTCTAATCTTCAACATGGGTGCTGGATACTGTGATGTAGCTATTACTGCTACAGCTGGAGGAGTTTCCCAAATAAAAGCCTTAGCAGGATCTGCCATTGGAGGGGAAGACTTGCTTCAGAGTATGATGCGCCATCTGTTGCCAGATTCCGAGACTCTTTTAACTAGCCACGGACTTGATGACATAAAGTCAGTTGGCATACTTCGAGTTGCAACACAGGAGGCAATCCACAATCTCTCCTCCCAAACTAGTGTGCAGATTGATGTTGACTTGGGAAATGGGACAAAAATATGTAAGGTTGTGGATCGGGAGGAATTTGAGGAAGTTAACCGAGCAGTGTTTGATAAGTGTGCGAGCCTCATAAGCCAGTGCTTGCATGATGCAAAGGTTGATACCGAGGATGTGAGCGATGTAATAGTTGTTGGCGGATGCTCATATATTCCAAAGATAAAAAGTCTTGTCATGAGCATTTGTAAAAAGGAGGAGCTGTACAAGGGGATGAAACCATTGGAAGCTGCTGTCATTGGAGCGGCACTAGAAGGAGCGGTGGCATCAGGTGTTAGCGATCCCTTTGGGAGTTTGGACTTGCTTACCATTCAAGCCACTCCTCTCGCCCTTGGGATCCGAGCTGATGGAAATAACTTTGTGCCTATCGTACCTAGAAACACAGCAATGCCAGCACAAAAAGATCTGATCTTCACAACTGCCAATGACAATCAAACTGAAGCACTAATAATCGTCTACGAAGGCGAGAGGAAGAAGGTGGAAGAGAACCATTTGCTGGGGTATTTCAAGATCACTGGAATTCCACCATCCCCGAAAGGAGTTCCGGAAATAAGAGTAATTCTGGACATCGATGCATCAAGCGTGCTGAGAGTTTTGGCCGGGGTATTAATGCCAGGGTCTCATCAGCCAGTAAATCCAGTCATGGGGGTAAGGATGCCAACAGTCGATGATGGTCACGGCTGGTGCGCTGAAGCCCTACACAGAGCTTATGGCTGTTCTCTAGACTTGGTTACGGTGCCGAAGAAGATGTAG

Protein Analysis

512

Amino Acids

55.05

Weight (kDa)

5.66

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 4 - 465 1.3e-86 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1519
AccB7I CCANNNNNTGG 1 cut(s) 1279
AccBSI CCGCTC 2 cut(s) 1028, 1043
AccI GTMKAC 1 cut(s) 1239
AccII CGCG 2 cut(s) 440, 452
AccIII TCCGGA 1 cut(s) 1327
AciI CCGC 6 cut(s) 7, 44, 310, 924, 1028, 1043
AclWI GGATC 8 cut(s) 131, 231, 493, 595, 810, 1058, 1114, 1127
AcoI YGGCCR 1 cut(s) 1380
AcsI RAATTY 2 cut(s) 812, 1302
AcuI CTGAAG 5 cut(s) 24, 270, 599, 1242, 1494
AfaI GTAC 3 cut(s) 408, 989, 1155
AfiI CCNNNNNNNGG 6 cut(s) 43, 44, 172, 600, 1279, 1427
AflIII ACRYGT 1 cut(s) 78
AgsI TTSAA 9 cut(s) 25, 96, 103, 179, 377, 465, 511, 1097, 1291
AhdI GACNNNNNGTC 1 cut(s) 685
AhlI ACTAGT 1 cut(s) 746
AjnI CCWGG 1 cut(s) 1396
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 2 cut(s) 644, 1407
AlwI GGATC 8 cut(s) 131, 231, 493, 595, 810, 1058, 1114, 1127
AlwNI CAGNNNCTG 1 cut(s) 590
Aor13HI TCCGGA 1 cut(s) 1327
AoxI GGCC 2 cut(s) 311, 1380
ApeKI GCWGC 2 cut(s) 212, 1013
ApoI RAATTY 2 cut(s) 812, 1302
AseI ATTAAT 2 cut(s) 116, 1391
Asp700I GAANNNNTTC 1 cut(s) 255
AspLEI GCGC 4 cut(s) 442, 454, 630, 1472
AspS9I GGNCC 1 cut(s) 312
AsuC2I CCSGG 1 cut(s) 1384
AsuHPI GGTGA 2 cut(s) 232, 325
AsuNHI GCTAGC 1 cut(s) 395
BamHI GGATCC 1 cut(s) 1119
BanI GGYRCC 1 cut(s) 1519
BbsI GAAGAC 1 cut(s) 612
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 2 cut(s) 224, 1000
BccI CCATC 4 cut(s) 639, 1124, 1318, 1448
BceAI ACGGC 1 cut(s) 1477
BciT130I CCWGG 1 cut(s) 1398
BciVI GTATCC 1 cut(s) 518
BcnI CCSGG 1 cut(s) 1384
BcoDI GTCTC 2 cut(s) 644, 1407
BcuI ACTAGT 1 cut(s) 746
BfaI CTAG 6 cut(s) 396, 663, 747, 1034, 1158, 1505
BfmI CTRYAG 1 cut(s) 549
BfuI GTATCC 1 cut(s) 518
BglII AGATCT 1 cut(s) 1186
BisI GCNGC 4 cut(s) 213, 311, 1014, 1029
BlsI GCNGC 4 cut(s) 214, 312, 1015, 1030
Bme1390I CCNGG 2 cut(s) 1384, 1398
BmeRI GACNNNNNGTC 1 cut(s) 685
BmgT120I GGNCC 1 cut(s) 312
BmiI GGNNCC 2 cut(s) 1121, 1521
BmrFI CCNGG 2 cut(s) 1384, 1398
BmsI GCATC 8 cut(s) 408, 615, 868, 917, 1058, 1345, 1367, 1428
BmtI GCTAGC 1 cut(s) 399
BpiI GAAGAC 1 cut(s) 612
BpmI CTGGAG 3 cut(s) 49, 108, 576
Bpu10I CCTNAGC 1 cut(s) 580
BpuEI CTTGAG 1 cut(s) 266
BpuMI CCSGG 1 cut(s) 1384
Bsa29I ATCGAT 1 cut(s) 1353
BsaI GGTCTC 1 cut(s) 1407
BsaJI CCNNGG 7 cut(s) 216, 390, 667, 894, 1114, 1383, 1397
BsaWI WCCGGW 2 cut(s) 482, 1327
BsaXI ACNNNNNCTCC 4 cut(s) 719, 749, 971, 1001
Bsc4I CCNNNNNNNGG 6 cut(s) 43, 44, 172, 600, 1279, 1427
Bse1I ACTGG 6 cut(s) 66, 140, 865, 1303, 1412, 1421
Bse3DI GCAATG 2 cut(s) 294, 1176
BseAI TCCGGA 1 cut(s) 1327
BseBI CCWGG 1 cut(s) 1398
BseCI ATCGAT 1 cut(s) 1353
BseDI CCNNGG 7 cut(s) 216, 390, 667, 894, 1114, 1383, 1397
BseGI GGATG 6 cut(s) 34, 904, 932, 1002, 1310, 1443
BseLI CCNNNNNNNGG 6 cut(s) 43, 44, 172, 600, 1279, 1427
BseMI GCAATG 2 cut(s) 294, 1176
BseMII CTCAG 3 cut(s) 424, 480, 1361
BseNI ACTGG 6 cut(s) 66, 140, 865, 1303, 1412, 1421
BseRI GAGGAG 4 cut(s) 573, 727, 995, 1095
BseXI GCAGC 2 cut(s) 224, 1000
BseYI CCCAGC 1 cut(s) 1279
BsgI GTGCAG 1 cut(s) 773
Bsh1236I CGCG 2 cut(s) 440, 452
Bsh1285I CGRYCG 1 cut(s) 11
BshFI GGCC 2 cut(s) 313, 1382
BshNI GGYRCC 1 cut(s) 1519
BshVI ATCGAT 1 cut(s) 1353
BsiEI CGRYCG 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 75
BsiSI CCGG 3 cut(s) 483, 1328, 1383
BslFI GGGAC 1 cut(s) 793
BslI CCNNNNNNNGG 6 cut(s) 43, 44, 172, 600, 1279, 1427
BsmAI GTCTC 2 cut(s) 644, 1407
BsmFI GGGAC 1 cut(s) 793
BsnI GGCC 2 cut(s) 313, 1382
Bso31I GGTCTC 1 cut(s) 1407
Bsp1286I GDGCHC 1 cut(s) 75
Bsp13I TCCGGA 1 cut(s) 1327
Bsp1407I TGTACA 1 cut(s) 987
Bsp19I CCATGG 1 cut(s) 390
BspACI CCGC 6 cut(s) 7, 44, 310, 924, 1028, 1043
BspANI GGCC 2 cut(s) 313, 1382
BspCNI CTCAG 3 cut(s) 423, 481, 1362
BspDI ATCGAT 1 cut(s) 1353
BspEI TCCGGA 1 cut(s) 1327
BspFNI CGCG 2 cut(s) 440, 452
BspHI TCATGA 1 cut(s) 960
BspLI GGNNCC 2 cut(s) 1121, 1521
BspOI GCTAGC 1 cut(s) 399
BspPI GGATC 8 cut(s) 131, 231, 493, 595, 810, 1058, 1114, 1127
BspT107I GGYRCC 1 cut(s) 1519
BspTNI GGTCTC 1 cut(s) 1407
BsrBI CCGCTC 2 cut(s) 1028, 1043
BsrDI GCAATG 2 cut(s) 294, 1176
BsrGI TGTACA 1 cut(s) 987
BsrI ACTGG 6 cut(s) 66, 140, 865, 1303, 1412, 1421
BssECI CCNNGG 7 cut(s) 216, 390, 667, 894, 1114, 1383, 1397
BssT1I CCWWGG 3 cut(s) 216, 390, 1114
Bst2UI CCWGG 1 cut(s) 1398
Bst4CI ACNGT 3 cut(s) 530, 1447, 1519
Bst6I CTCTTC 1 cut(s) 1260
BstAPI GCANNNNNTGC 1 cut(s) 590
BstAUI TGTACA 1 cut(s) 987
BstC8I GCNNGC 5 cut(s) 397, 853, 872, 1176, 1367
BstDEI CTNAG 4 cut(s) 410, 489, 580, 1370
BstDSI CCRYGG 2 cut(s) 390, 667
BstF5I GGATG 6 cut(s) 34, 904, 932, 1002, 1310, 1443
BstFNI CGCG 2 cut(s) 440, 452
BstHHI GCGC 4 cut(s) 442, 454, 630, 1472
BstMAI GTCTC 2 cut(s) 644, 1407
BstMCI CGRYCG 1 cut(s) 11
BstMWI GCNNNNNNNGC 5 cut(s) 310, 545, 590, 861, 1175
BstNI CCWGG 1 cut(s) 1398
BstNSI RCATGY 1 cut(s) 82
BstSCI CCNGG 2 cut(s) 1382, 1396
BstSFI CTRYAG 1 cut(s) 549
BstUI CGCG 2 cut(s) 440, 452
BstV1I GCAGC 2 cut(s) 224, 1000
BstV2I GAAGAC 1 cut(s) 612
BstX2I RGATCY 5 cut(s) 236, 485, 587, 1119, 1186
BstYI RGATCY 5 cut(s) 236, 485, 587, 1119, 1186
Bsu15I ATCGAT 1 cut(s) 1353
BsuI GTATCC 1 cut(s) 518
BsuRI GGCC 2 cut(s) 313, 1382
BsuTUI ATCGAT 1 cut(s) 1353
BtgI CCRYGG 2 cut(s) 390, 667
BtgZI GCGATG 1 cut(s) 921
BtsCI GGATG 6 cut(s) 34, 904, 932, 1002, 1310, 1443
BtsI GCAGTG 1 cut(s) 840
BtsIMutI CAGTG 5 cut(s) 73, 203, 840, 872, 1296
Cac8I GCNNGC 5 cut(s) 397, 853, 872, 1176, 1367
CaiI CAGNNNCTG 1 cut(s) 590
CciI TCATGA 1 cut(s) 960
CfoI GCGC 4 cut(s) 442, 454, 630, 1472
Cfr13I GGNCC 1 cut(s) 312
ClaI ATCGAT 1 cut(s) 1353
CseI GACGC 1 cut(s) 188
Csp6I GTAC 3 cut(s) 407, 988, 1154
CviAII CATG 8 cut(s) 79, 106, 391, 404, 514, 875, 961, 1426
CviQI GTAC 3 cut(s) 407, 988, 1154
DdeI CTNAG 4 cut(s) 410, 489, 580, 1370
DriI GACNNNNNGTC 1 cut(s) 685
EaeI YGGCCR 1 cut(s) 1380
Eam1104I CTCTTC 1 cut(s) 1260
Eam1105I GACNNNNNGTC 1 cut(s) 685
EarI CTCTTC 1 cut(s) 1260
EciI GGCGGA 1 cut(s) 939
Eco130I CCWWGG 3 cut(s) 216, 390, 1114
Eco31I GGTCTC 1 cut(s) 1407
Eco57I CTGAAG 5 cut(s) 24, 270, 599, 1242, 1494
EcoRI GAATTC 1 cut(s) 1302
EcoRII CCWGG 1 cut(s) 1396
EcoT14I CCWWGG 3 cut(s) 216, 390, 1114
EcoT22I ATGCAT 1 cut(s) 1360
ErhI CCWWGG 3 cut(s) 216, 390, 1114
FaeI CATG 8 cut(s) 82, 109, 394, 407, 517, 878, 964, 1429
FalI AAGNNNNNCTT 2 cut(s) 597, 629
FaqI GGGAC 1 cut(s) 793
FatI CATG 8 cut(s) 78, 105, 390, 403, 513, 874, 960, 1425
FauI CCCGC 1 cut(s) 37
FblI GTMKAC 1 cut(s) 1239
Fnu4HI GCNGC 4 cut(s) 213, 311, 1014, 1029
FokI GGATG 6 cut(s) 41, 911, 939, 1009, 1297, 1450
Fsp4HI GCNGC 4 cut(s) 213, 311, 1014, 1029
FspBI CTAG 6 cut(s) 396, 663, 747, 1034, 1158, 1505
GlaI GCGC 4 cut(s) 441, 453, 629, 1471
GluI GCNGC 4 cut(s) 213, 311, 1014, 1029
GsaI CCCAGC 1 cut(s) 1283
GsuI CTGGAG 3 cut(s) 49, 108, 576
HaeIII GGCC 2 cut(s) 313, 1382
HapII CCGG 3 cut(s) 483, 1328, 1383
HgaI GACGC 1 cut(s) 188
HhaI GCGC 4 cut(s) 442, 454, 630, 1472
Hin1II CATG 8 cut(s) 82, 109, 394, 407, 517, 878, 964, 1429
Hin6I GCGC 4 cut(s) 440, 452, 628, 1470
HinP1I GCGC 4 cut(s) 440, 452, 628, 1470
HincII GTYRAC 3 cut(s) 130, 766, 826
HindII GTYRAC 3 cut(s) 130, 766, 826
HinfI GANTC 4 cut(s) 357, 367, 644, 652
HpaI GTTAAC 1 cut(s) 826
HpaII CCGG 3 cut(s) 483, 1328, 1383
HphI GGTGA 2 cut(s) 232, 325
Hpy166II GTNNAC 7 cut(s) 130, 203, 223, 478, 766, 826, 1240
Hpy188I TCNGA 7 cut(s) 413, 471, 490, 618, 649, 1124, 1191
Hpy188III TCNNGA 8 cut(s) 87, 248, 806, 961, 1291, 1328, 1346, 1505
Hpy8I GTNNAC 7 cut(s) 130, 203, 223, 478, 766, 826, 1240
HpyAV CCTTC 5 cut(s) 48, 410, 1031, 1238, 1252
HpyCH4III ACNGT 3 cut(s) 530, 1447, 1519
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 5 cut(s) 710, 754, 874, 881, 1358
HpyF10VI GCNNNNNNNGC 5 cut(s) 310, 545, 590, 861, 1175
HpyF3I CTNAG 4 cut(s) 410, 489, 580, 1370
HpySE526I ACGT 1 cut(s) 474
Hsp92II CATG 8 cut(s) 82, 109, 394, 407, 517, 878, 964, 1429
HspAI GCGC 4 cut(s) 440, 452, 628, 1470
Kpn2I TCCGGA 1 cut(s) 1327
KspAI GTTAAC 1 cut(s) 826
LmnI GCTCC 4 cut(s) 89, 982, 1025, 1040
Lsp1109I GCAGC 2 cut(s) 224, 1000
LweI GCATC 8 cut(s) 408, 615, 868, 917, 1058, 1345, 1367, 1428
MaeI CTAG 6 cut(s) 396, 663, 747, 1034, 1158, 1505
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 3 cut(s) 16, 1457, 1513
MbiI CCGCTC 2 cut(s) 1028, 1043
MboII GAAGA 8 cut(s) 91, 263, 499, 505, 617, 1186, 1267, 1277
MflI RGATCY 5 cut(s) 236, 485, 587, 1119, 1186
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 4 cut(s) 117, 812, 1302, 1341
MlyI GAGTC 2 cut(s) 361, 646
Mph1103I ATGCAT 1 cut(s) 1360
MroI TCCGGA 1 cut(s) 1327
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 4 cut(s) 116, 659, 825, 1391
MslI CAYNNNNRTG 2 cut(s) 194, 1169
MspA1I CMGCKG 3 cut(s) 44, 364, 554
MspI CCGG 3 cut(s) 483, 1328, 1383
MspR9I CCNGG 2 cut(s) 1384, 1398
MvaI CCWGG 1 cut(s) 1398
MvnI CGCG 2 cut(s) 440, 452
MwoI GCNNNNNNNGC 5 cut(s) 310, 545, 590, 861, 1175
NciI CCSGG 1 cut(s) 1384
NcoI CCATGG 1 cut(s) 390
NheI GCTAGC 1 cut(s) 395
NlaIII CATG 8 cut(s) 82, 109, 394, 407, 517, 878, 964, 1429
NlaIV GGNNCC 2 cut(s) 1121, 1521
NmuCI GTSAC 2 cut(s) 16, 1457
NsiI ATGCAT 1 cut(s) 1360
NspI RCATGY 1 cut(s) 82
PagI TCATGA 1 cut(s) 960
PciI ACATGT 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 2 cut(s) 357, 644
PflMI CCANNNNNTGG 1 cut(s) 1279
PkrI GCNGC 4 cut(s) 214, 312, 1015, 1030
PleI GAGTC 2 cut(s) 361, 646
PpsI GAGTC 2 cut(s) 361, 646
PscI ACATGT 1 cut(s) 78
PshBI ATTAAT 2 cut(s) 116, 1391
Psp6I CCWGG 1 cut(s) 1396
PspFI CCCAGC 1 cut(s) 1279
PspGI CCWGG 1 cut(s) 1396
PspN4I GGNNCC 2 cut(s) 1121, 1521
PspPI GGNCC 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 590
PsuI RGATCY 5 cut(s) 236, 485, 587, 1119, 1186
PvuII CAGCTG 2 cut(s) 364, 554
RsaI GTAC 3 cut(s) 408, 989, 1155
RsaNI GTAC 3 cut(s) 407, 988, 1154
RseI CAYNNNNRTG 2 cut(s) 194, 1169
SaqAI TTAA 4 cut(s) 116, 659, 825, 1391
SatI GCNGC 4 cut(s) 213, 311, 1014, 1029
Sau96I GGNCC 1 cut(s) 312
SchI GAGTC 2 cut(s) 361, 646
ScrFI CCNGG 2 cut(s) 1384, 1398
SduI GDGCHC 1 cut(s) 75
SfaNI GCATC 8 cut(s) 408, 615, 868, 917, 1058, 1345, 1367, 1428
SfcI CTRYAG 1 cut(s) 549
SmiMI CAYNNNNRTG 2 cut(s) 194, 1169
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
SpeI ACTAGT 1 cut(s) 746
Sse9I AATT 4 cut(s) 117, 812, 1302, 1341
SsiI CCGC 6 cut(s) 7, 44, 310, 924, 1028, 1043
SspI AATATT 1 cut(s) 267
SspMI CTAG 6 cut(s) 396, 663, 747, 1034, 1158, 1505
StyD4I CCNGG 2 cut(s) 1382, 1396
StyI CCWWGG 3 cut(s) 216, 390, 1114
TaaI ACNGT 3 cut(s) 530, 1447, 1519
TaiI ACGT 1 cut(s) 477
TaqI TCGA 3 cut(s) 703, 1353, 1449
TasI AATT 4 cut(s) 117, 812, 1302, 1341
TatI WGTACW 2 cut(s) 406, 987
TauI GCSGC 2 cut(s) 313, 1031
TfiI GAWTC 2 cut(s) 357, 644
Tru1I TTAA 4 cut(s) 116, 659, 825, 1391
Tru9I TTAA 4 cut(s) 116, 659, 825, 1391
TscAI CASTG 5 cut(s) 73, 210, 840, 872, 1303
TseFI GTSAC 2 cut(s) 16, 1457
TseI GCWGC 2 cut(s) 212, 1013
Tsp45I GTSAC 2 cut(s) 16, 1457
TspDTI ATGAA 5 cut(s) 45, 122, 336, 392, 1013
TspGWI ACGGA 1 cut(s) 684
TspRI CASTG 5 cut(s) 73, 210, 840, 872, 1303
Van91I CCANNNNNTGG 1 cut(s) 1279
VspI ATTAAT 2 cut(s) 116, 1391
XapI RAATTY 2 cut(s) 812, 1302
XbaI TCTAGA 1 cut(s) 1504
XceI RCATGY 1 cut(s) 82
XmiI GTMKAC 1 cut(s) 1239
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 6 cut(s) 396, 663, 747, 1034, 1158, 1505
Zsp2I ATGCAT 1 cut(s) 1360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.