RLG00000012228
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009614
Physical Location & Seq
Reverse (-)
16428602 .. 16432326
3725 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012228

Sequence Viewer

Length: 1716 bp
ATGGCTGAGGTAGCATACACTGTGGCATCTGATAGTGAAACCAGTCTGGAGGAGAAATCCTCATGCACCTTTCCTGAAACAACAATTGGAATTGATATTGGTACCTCACAATGCAGGGTTGCGATCTGGAATGGCTCCCAGGTTGAGATCCTTAAAAACAGTAGGAACCAGAGGATGATGAGTTCATATGTTACATTCAAGGATGGAACTCCTTCTGGGGGACTAAGCAATCAACTCTCTGCAGATCATGATATATTATCTGGAGCTGCAATATTCAACATGAAACGCTTAATTGGCCGAGTTGACACGGATCCAGTTGTCCACTCTAGCAAAAGCCTTCCATTTCTAGTACAAACATTGGACATCGGTGTCCGTCCATTTATTGCAGCCTTGGTTGGAAATTTCTGGAGATCCACCACTCCTGAAGAAGTTCTGGCAATATTTTTGGTGGAACTTAGGGAAATGGCAGAAATTCAGTTAAAGCGGCCCATAAAAAATGTTGTTATGACTGTGCCAGTTTCATTCAGCCGATTCCAGCTGACTCGGATTGAGAGAGCATGTGCCATGGCTGGCCTTCATATCCTGAAACTGATGCCAGAACCAACTGCAGTGGCGTTGTTATATACCTTACATACAAAACAAAGGGGATTTCAGTCGACAGATAGGAATGTTCTAATCTTCAATATGGGTGCAGGATACTGTGATGTTGCCATAAGTGTGACAGCTGAAGAGGCACCACGAGGAATTTCCGAAATAATTGCCTTAACAGGAAGCGCCATTGGAGGAGAAGACTTGCTTCAGAATATGATGCGTCATCTGTTGCCAAATGCAGAGAATCTTTTGACAAGTCATGGAGTTGACGAAATTAAATCAAAGGGGTTACTTCGAGTTGCAACGCAGAATGCAATCCAGAGTCTCTCCTCCCAAAATTCTGTTCAAATTGATGTTGACTTGGGAAATGGGATGAAAATCTGCAAGGTTGTGGACCGAGAAGAATTTGAGGAAGTTAATATTGCAGTGTTTGATAAGTGTGCTAGCCTCATAATCCAGTGTTTGTATGATGCAAAGGTAAAACCTGAGGATGTGAACGATGTAGTACTTGTAGGTGGATGTTCATACATTCCAAAGATAAAAAATCTTGTCATGAGCGTATGCAAGAAAGAGGAGCTTTACGAAGGGATGAACCCATTGGAAGCTGCTGTCTCTGGAGCAGCACTACAAGGATATGTAGCTTCAGGTGTTCATGATCCATTTGAATCCGTGGATGTGCTATCTATTCAGGTCACACCTCTTTCGATTGGAATTCGAGCTGATGGAAACAACTTTGTGCCGATCATACCTAGAAACACCACAATACCAGCCCAAAGAGAGATGCTTTTCACAACATCCCATGATAATCAAGCTGAAGCACTTATAATTGTTTATGAAGGTGATGGCAAGAAGCTGGAAGAAAATCATTTGCTGGGATACTTCAAAATTGAGGGAATTCCTCCAGCAACCAAGGGAGTTCCACAAATGCGAGTAATGATGGACATTGATGCTTCAAACGTGCTGAGAGTTTTGGCTGCTGTGTTGATGCCAGGTTCTCATCTTCCAATAAATCCTGTCCTGGACGTGAGGATGCCAACGGTTGACGATGGTCATGCCTGGTGCGCGGAAGCCTTGCACAGAATTTATGGTGCTACTCAAGACTTGGTATCAGTGAAGAGGATCTAG

Protein Analysis

572

Amino Acids

62.32

Weight (kDa)

5.36

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 29 - 525 3.8e-97 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1415
AasI GACNNNNNNGTC 1 cut(s) 368
Acc65I GGTACC 1 cut(s) 101
AccB1I GGYRCC 2 cut(s) 101, 733
AccI GTMKAC 1 cut(s) 656
AccII CGCG 1 cut(s) 1655
AciI CCGC 2 cut(s) 484, 1655
AclWI GGATC 5 cut(s) 142, 305, 318, 405, 1241
AcoI YGGCCR 1 cut(s) 295
AcsI RAATTY 8 cut(s) 400, 471, 744, 928, 995, 1302, 1485, 1671
AcuI CTGAAG 5 cut(s) 444, 747, 782, 1218, 1425
AfaI GTAC 3 cut(s) 103, 351, 1098
AfiI CCNNNNNNNGG 1 cut(s) 218
AgsI TTSAA 7 cut(s) 199, 277, 682, 938, 1256, 1474, 1545
AjiI CACGTC 1 cut(s) 1615
AjnI CCWGG 4 cut(s) 138, 1579, 1608, 1646
AjuI GAANNNNNNNTTGG 4 cut(s) 69, 101, 918, 950
AloI GAACNNNNNNTCC 2 cut(s) 166, 198
AluBI AGCT 9 cut(s) 266, 538, 725, 1168, 1196, 1232, 1310, 1403, 1444
AluI AGCT 9 cut(s) 266, 538, 725, 1168, 1196, 1232, 1310, 1403, 1444
Alw26I GTCTC 2 cut(s) 920, 1207
AlwI GGATC 5 cut(s) 142, 305, 318, 405, 1241
AoxI GGCC 3 cut(s) 295, 485, 571
ApeKI GCWGC 5 cut(s) 266, 386, 1196, 1211, 1565
ApoI RAATTY 8 cut(s) 400, 471, 744, 928, 995, 1302, 1485, 1671
Asp700I GAANNNNTTC 2 cut(s) 211, 429
Asp718I GGTACC 1 cut(s) 101
AspLEI GCGC 2 cut(s) 776, 1655
AspS9I GGNCC 2 cut(s) 486, 985
AsuHPI GGTGA 1 cut(s) 1442
AsuNHI GCTAGC 1 cut(s) 1034
AvaII GGWCC 1 cut(s) 985
AxyI CCTNAGG 1 cut(s) 1077
BamHI GGATCC 1 cut(s) 310
BanI GGYRCC 2 cut(s) 101, 733
BauI CACGAG 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 795
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 5 cut(s) 253, 398, 1183, 1223, 1552
BccI CCATC 5 cut(s) 197, 1307, 1427, 1522, 1631
BcgI CGANNNNNNTGC 4 cut(s) 740, 774, 1625, 1659
BciT130I CCWGG 4 cut(s) 140, 1581, 1610, 1648
BciVI GTATCC 2 cut(s) 689, 1460
BcoDI GTCTC 2 cut(s) 920, 1207
BfaI CTAG 5 cut(s) 327, 347, 1035, 1341, 1714
BfmI CTRYAG 2 cut(s) 240, 606
BfoI RGCGCY 1 cut(s) 777
BfuI GTATCC 2 cut(s) 689, 1460
BisI GCNGC 6 cut(s) 267, 387, 485, 1197, 1212, 1566
BlsI GCNGC 6 cut(s) 268, 388, 486, 1198, 1213, 1567
BmcAI AGTACT 1 cut(s) 1098
Bme1390I CCNGG 4 cut(s) 140, 1581, 1610, 1648
Bme18I GGWCC 1 cut(s) 985
BmgBI CACGTC 1 cut(s) 1615
BmgT120I GGNCC 2 cut(s) 486, 985
BmiI GGNNCC 5 cut(s) 103, 136, 167, 312, 735
BmrFI CCNGG 4 cut(s) 140, 1581, 1610, 1648
BmsI GCATC 8 cut(s) 35, 582, 798, 1051, 1362, 1528, 1566, 1611
BmtI GCTAGC 1 cut(s) 1038
BoxI GACNNNNGTC 1 cut(s) 1638
BpiI GAAGAC 1 cut(s) 795
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpmI CTGGAG 5 cut(s) 68, 282, 427, 1227, 1476
Bpu10I CCTNAGC 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 1671
BsaBI GATNNNNATC 1 cut(s) 968
BsaJI CCNNGG 5 cut(s) 138, 390, 564, 1260, 1500
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse1I ACTGG 4 cut(s) 42, 314, 515, 1048
Bse21I CCTNAGG 1 cut(s) 1077
Bse8I GATNNNNATC 1 cut(s) 968
BseBI CCWGG 4 cut(s) 140, 1581, 1610, 1648
BseDI CCNNGG 5 cut(s) 138, 390, 564, 1260, 1500
BseGI GGATG 9 cut(s) 180, 208, 969, 1087, 1115, 1185, 1270, 1385, 1626
BseJI GATNNNNATC 1 cut(s) 968
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMII CTCAG 2 cut(s) 1068, 1544
BseNI ACTGG 4 cut(s) 42, 314, 515, 1048
BseRI GAGGAG 4 cut(s) 65, 798, 910, 1178
BseXI GCAGC 5 cut(s) 253, 398, 1183, 1223, 1552
BseYI CCCAGC 1 cut(s) 1462
BsgI GTGCAG 1 cut(s) 711
Bsh1236I CGCG 1 cut(s) 1655
BshFI GGCC 3 cut(s) 297, 487, 573
BshNI GGYRCC 2 cut(s) 101, 733
BslFI GGGAC 1 cut(s) 234
BslI CCNNNNNNNGG 1 cut(s) 218
BsmAI GTCTC 2 cut(s) 920, 1207
BsmFI GGGAC 1 cut(s) 234
BsmI GAATGC 1 cut(s) 907
BsnI GGCC 3 cut(s) 297, 487, 573
Bsp143I GATC 8 cut(s) 123, 147, 244, 310, 410, 1246, 1332, 1710
Bsp19I CCATGG 1 cut(s) 564
BspACI CCGC 2 cut(s) 484, 1655
BspANI GGCC 3 cut(s) 297, 487, 573
BspCNI CTCAG 2 cut(s) 1069, 1545
BspFNI CGCG 1 cut(s) 1655
BspHI TCATGA 3 cut(s) 247, 1143, 1243
BspLI GGNNCC 5 cut(s) 103, 136, 167, 312, 735
BspMAI CTGCAG 2 cut(s) 244, 610
BspOI GCTAGC 1 cut(s) 1038
BspPI GGATC 5 cut(s) 142, 305, 318, 405, 1241
BspT107I GGYRCC 2 cut(s) 101, 733
BsrI ACTGG 4 cut(s) 42, 314, 515, 1048
BssECI CCNNGG 5 cut(s) 138, 390, 564, 1260, 1500
BssMI GATC 8 cut(s) 123, 147, 244, 310, 410, 1246, 1332, 1710
BssSI CACGAG 1 cut(s) 738
BssT1I CCWWGG 3 cut(s) 390, 564, 1500
Bst2BI CACGAG 1 cut(s) 738
Bst2UI CCWGG 4 cut(s) 140, 1581, 1610, 1648
Bst4CI ACNGT 5 cut(s) 22, 161, 511, 701, 1630
Bst6I CTCTTC 2 cut(s) 723, 1700
BstC8I GCNNGC 2 cut(s) 571, 1036
BstDEI CTNAG 5 cut(s) 6, 224, 455, 1077, 1553
BstDSI CCRYGG 2 cut(s) 564, 1260
BstF5I GGATG 9 cut(s) 180, 208, 969, 1087, 1115, 1185, 1270, 1385, 1626
BstFNI CGCG 1 cut(s) 1655
BstH2I RGCGCY 1 cut(s) 777
BstHHI GCGC 2 cut(s) 776, 1655
BstKTI GATC 8 cut(s) 126, 150, 247, 313, 413, 1249, 1335, 1713
BstMAI GTCTC 2 cut(s) 920, 1207
BstMBI GATC 8 cut(s) 123, 147, 244, 310, 410, 1246, 1332, 1710
BstMWI GCNNNNNNNGC 4 cut(s) 11, 294, 731, 1652
BstNI CCWGG 4 cut(s) 140, 1581, 1610, 1648
BstNSI RCATGY 1 cut(s) 561
BstPAI GACNNNNGTC 1 cut(s) 1638
BstSCI CCNGG 4 cut(s) 138, 1579, 1608, 1646
BstSFI CTRYAG 2 cut(s) 240, 606
BstUI CGCG 1 cut(s) 1655
BstV1I GCAGC 5 cut(s) 253, 398, 1183, 1223, 1552
BstV2I GAAGAC 1 cut(s) 795
BstX2I RGATCY 4 cut(s) 147, 310, 410, 1710
BstYI RGATCY 4 cut(s) 147, 310, 410, 1710
Bsu36I CCTNAGG 1 cut(s) 1077
BsuI GTATCC 2 cut(s) 689, 1460
BsuRI GGCC 3 cut(s) 297, 487, 573
BtgI CCRYGG 2 cut(s) 564, 1260
BtrI CACGTC 1 cut(s) 1615
BtsCI GGATG 9 cut(s) 180, 208, 969, 1087, 1115, 1185, 1270, 1385, 1626
BtsI GCAGTG 2 cut(s) 615, 1023
BtsIMutI CAGTG 5 cut(s) 18, 615, 1023, 1055, 1707
Cac8I GCNNGC 2 cut(s) 571, 1036
CciI TCATGA 3 cut(s) 247, 1143, 1243
CfoI GCGC 2 cut(s) 776, 1655
Cfr13I GGNCC 2 cut(s) 486, 985
CseI GACGC 1 cut(s) 800
Csp6I GTAC 3 cut(s) 102, 350, 1097
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviQI GTAC 3 cut(s) 102, 350, 1097
DdeI CTNAG 5 cut(s) 6, 224, 455, 1077, 1553
DpnI GATC 8 cut(s) 125, 149, 246, 312, 412, 1248, 1334, 1712
DpnII GATC 8 cut(s) 123, 147, 244, 310, 410, 1246, 1332, 1710
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 1 cut(s) 295
Eam1104I CTCTTC 2 cut(s) 723, 1700
EarI CTCTTC 2 cut(s) 723, 1700
Eco130I CCWWGG 3 cut(s) 390, 564, 1500
Eco47I GGWCC 1 cut(s) 985
Eco57I CTGAAG 5 cut(s) 444, 747, 782, 1218, 1425
Eco81I CCTNAGG 1 cut(s) 1077
EcoRI GAATTC 2 cut(s) 1302, 1485
EcoRII CCWGG 4 cut(s) 138, 1579, 1608, 1646
EcoT14I CCWWGG 3 cut(s) 390, 564, 1500
ErhI CCWWGG 3 cut(s) 390, 564, 1500
FalI AAGNNNNNCTT 4 cut(s) 780, 812, 1152, 1184
FaqI GGGAC 1 cut(s) 234
FauNDI CATATG 1 cut(s) 187
FblI GTMKAC 1 cut(s) 656
Fnu4HI GCNGC 6 cut(s) 267, 387, 485, 1197, 1212, 1566
FokI GGATG 9 cut(s) 187, 215, 976, 1094, 1122, 1192, 1277, 1372, 1633
Fsp4HI GCNGC 6 cut(s) 267, 387, 485, 1197, 1212, 1566
FspBI CTAG 5 cut(s) 327, 347, 1035, 1341, 1714
GlaI GCGC 2 cut(s) 775, 1654
GluI GCNGC 6 cut(s) 267, 387, 485, 1197, 1212, 1566
GsaI CCCAGC 1 cut(s) 1466
GsuI CTGGAG 5 cut(s) 68, 282, 427, 1227, 1476
HaeII RGCGCY 1 cut(s) 777
HaeIII GGCC 3 cut(s) 297, 487, 573
HgaI GACGC 1 cut(s) 800
HhaI GCGC 2 cut(s) 776, 1655
Hin6I GCGC 2 cut(s) 774, 1653
HinP1I GCGC 2 cut(s) 774, 1653
HincII GTYRAC 5 cut(s) 304, 657, 859, 949, 1633
HindII GTYRAC 5 cut(s) 304, 657, 859, 949, 1633
HinfI GANTC 5 cut(s) 531, 541, 835, 913, 1256
HphI GGTGA 1 cut(s) 1442
Hpy166II GTNNAC 8 cut(s) 304, 322, 657, 859, 949, 985, 1087, 1633
Hpy188I TCNGA 4 cut(s) 31, 546, 751, 801
Hpy8I GTNNAC 8 cut(s) 304, 322, 657, 859, 949, 985, 1087, 1633
HpyAV CCTTC 5 cut(s) 222, 347, 584, 1169, 1421
HpyCH4III ACNGT 5 cut(s) 22, 161, 511, 701, 1630
HpyCH4IV ACGT 2 cut(s) 1548, 1614
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 294, 731, 1652
HpyF3I CTNAG 5 cut(s) 6, 224, 455, 1077, 1553
HpySE526I ACGT 2 cut(s) 1548, 1614
HspAI GCGC 2 cut(s) 774, 1653
KpnI GGTACC 1 cut(s) 105
Kzo9I GATC 8 cut(s) 123, 147, 244, 310, 410, 1246, 1332, 1710
LmnI GCTCC 4 cut(s) 140, 263, 1165, 1208
Lsp1109I GCAGC 5 cut(s) 253, 398, 1183, 1223, 1552
LweI GCATC 8 cut(s) 35, 582, 798, 1051, 1362, 1528, 1566, 1611
MaeI CTAG 5 cut(s) 327, 347, 1035, 1341, 1714
MaeII ACGT 2 cut(s) 1548, 1614
MaeIII GTNAC 4 cut(s) 190, 718, 879, 1282
MalI GATC 8 cut(s) 125, 149, 246, 312, 412, 1248, 1334, 1712
MboI GATC 8 cut(s) 123, 147, 244, 310, 410, 1246, 1332, 1710
MboII GAAGA 7 cut(s) 437, 670, 740, 800, 1004, 1460, 1583
MfeI CAATTG 1 cut(s) 84
MflI RGATCY 4 cut(s) 147, 310, 410, 1710
MlyI GAGTC 2 cut(s) 535, 922
MmeI TCCRAC 1 cut(s) 376
MroXI GAANNNNTTC 2 cut(s) 211, 429
MseI TTAA 6 cut(s) 153, 290, 479, 764, 867, 1008
MslI CAYNNNNRTG 1 cut(s) 716
MspA1I CMGCKG 2 cut(s) 538, 725
MspR9I CCNGG 4 cut(s) 140, 1581, 1610, 1648
MunI CAATTG 1 cut(s) 84
Mva1269I GAATGC 1 cut(s) 907
MvaI CCWGG 4 cut(s) 140, 1581, 1610, 1648
MvnI CGCG 1 cut(s) 1655
MwoI GCNNNNNNNGC 4 cut(s) 11, 294, 731, 1652
NcoI CCATGG 1 cut(s) 564
NdeI CATATG 1 cut(s) 187
NdeII GATC 8 cut(s) 123, 147, 244, 310, 410, 1246, 1332, 1710
NheI GCTAGC 1 cut(s) 1034
NlaIV GGNNCC 5 cut(s) 103, 136, 167, 312, 735
NmeAIII GCCGAG 1 cut(s) 323
NmuCI GTSAC 2 cut(s) 718, 1282
NspI RCATGY 1 cut(s) 561
PagI TCATGA 3 cut(s) 247, 1143, 1243
PctI GAATGC 1 cut(s) 907
PdmI GAANNNNTTC 2 cut(s) 211, 429
PfeI GAWTC 3 cut(s) 531, 835, 1256
PfoI TCCNGGA 1 cut(s) 1608
PkrI GCNGC 6 cut(s) 268, 388, 486, 1198, 1213, 1567
PleI GAGTC 2 cut(s) 535, 921
PpsI GAGTC 2 cut(s) 535, 921
PshAI GACNNNNGTC 1 cut(s) 1638
PsiI TTATAA 1 cut(s) 1415
Psp6I CCWGG 4 cut(s) 138, 1579, 1608, 1646
PspFI CCCAGC 1 cut(s) 1462
PspGI CCWGG 4 cut(s) 138, 1579, 1608, 1646
PspN4I GGNNCC 5 cut(s) 103, 136, 167, 312, 735
PspPI GGNCC 2 cut(s) 486, 985
PstI CTGCAG 2 cut(s) 244, 610
PsuI RGATCY 4 cut(s) 147, 310, 410, 1710
PvuII CAGCTG 2 cut(s) 538, 725
RsaI GTAC 3 cut(s) 103, 351, 1098
RsaNI GTAC 3 cut(s) 102, 350, 1097
RseI CAYNNNNRTG 1 cut(s) 716
SalI GTCGAC 1 cut(s) 655
SaqAI TTAA 6 cut(s) 153, 290, 479, 764, 867, 1008
SatI GCNGC 6 cut(s) 267, 387, 485, 1197, 1212, 1566
Sau3AI GATC 8 cut(s) 123, 147, 244, 310, 410, 1246, 1332, 1710
Sau96I GGNCC 2 cut(s) 486, 985
ScaI AGTACT 1 cut(s) 1098
SchI GAGTC 2 cut(s) 535, 922
ScrFI CCNGG 4 cut(s) 140, 1581, 1610, 1648
SfaNI GCATC 8 cut(s) 35, 582, 798, 1051, 1362, 1528, 1566, 1611
SfcI CTRYAG 2 cut(s) 240, 606
SinI GGWCC 1 cut(s) 985
SmiMI CAYNNNNRTG 1 cut(s) 716
SmlI CTYRAG 1 cut(s) 1686
SmoI CTYRAG 1 cut(s) 1686
SsiI CCGC 2 cut(s) 484, 1655
SspI AATATT 3 cut(s) 273, 441, 1012
SspMI CTAG 5 cut(s) 327, 347, 1035, 1341, 1714
StyD4I CCNGG 4 cut(s) 138, 1579, 1608, 1646
StyI CCWWGG 3 cut(s) 390, 564, 1500
TaaI ACNGT 5 cut(s) 22, 161, 511, 701, 1630
TaiI ACGT 2 cut(s) 1551, 1617
TaqI TCGA 4 cut(s) 656, 886, 1295, 1306
TaqII GACCGA 1 cut(s) 1002
TatI WGTACW 2 cut(s) 349, 1096
TauI GCSGC 1 cut(s) 487
TfiI GAWTC 3 cut(s) 531, 835, 1256
Tru1I TTAA 6 cut(s) 153, 290, 479, 764, 867, 1008
Tru9I TTAA 6 cut(s) 153, 290, 479, 764, 867, 1008
TscAI CASTG 5 cut(s) 25, 615, 1023, 1055, 1707
TseFI GTSAC 2 cut(s) 718, 1282
TseI GCWGC 5 cut(s) 266, 386, 1196, 1211, 1565
Tsp45I GTSAC 2 cut(s) 718, 1282
TspDTI ATGAA 9 cut(s) 174, 296, 510, 566, 980, 1104, 1196, 1232, 1440
TspGWI ACGGA 3 cut(s) 323, 362, 1249
TspRI CASTG 5 cut(s) 25, 615, 1023, 1055, 1707
VpaK11BI GGWCC 1 cut(s) 985
XapI RAATTY 8 cut(s) 400, 471, 744, 928, 995, 1302, 1485, 1671
XceI RCATGY 1 cut(s) 561
XmiI GTMKAC 1 cut(s) 656
XmnI GAANNNNTTC 2 cut(s) 211, 429
XspI CTAG 5 cut(s) 327, 347, 1035, 1341, 1714
ZrmI AGTACT 1 cut(s) 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.